WO1992007061A1 - Recipient de milieux de culture et son utilisation - Google Patents

Recipient de milieux de culture et son utilisation Download PDF

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Publication number
WO1992007061A1
WO1992007061A1 PCT/EP1991/001953 EP9101953W WO9207061A1 WO 1992007061 A1 WO1992007061 A1 WO 1992007061A1 EP 9101953 W EP9101953 W EP 9101953W WO 9207061 A1 WO9207061 A1 WO 9207061A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
fresnel lens
lid
container according
designed
Prior art date
Application number
PCT/EP1991/001953
Other languages
German (de)
English (en)
Inventor
Fritz Reulecke
Thomas Hackel
Original Assignee
Fritz Reulecke
Thomas Hackel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fritz Reulecke, Thomas Hackel filed Critical Fritz Reulecke
Publication of WO1992007061A1 publication Critical patent/WO1992007061A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters

Definitions

  • the invention relates to a container for culture media with a transparent container section for visual examination of the container contents.
  • a container for culture media When growing cell cultures and microorganisms, crystal-clear, thin-walled plastic containers are used that are sterilized and used as single-use items.
  • Culture media in the form of liquids for cell cultures are often stored in cell culture bottles, as are known, for example, from DE-OS 36 28 930. The growth of the cultures in the culture broth is monitored by the transparent walls.
  • Solid nutrient media in the form of agar or nutrient pad discs are usually stored in relatively flat dishes or in nutrient media holders.
  • Such containers for culture media are either designed as circular petri dishes or have the form of a culture medium holder with a handle and a case enclosing the culture media against contamination, through which the growth of the microorganisms can be observed.
  • the containers for culture media listed above have also included filter elements with the aid of which liquids can be filtered and the microorganisms contained therein can be retained.
  • plano-convex magnifying glass Apart from the fact that the material costs increase due to the greater plastic requirement, the material thickening to form a plano-convex magnifying glass also has the disadvantage that the partial thickening of the thin plastic walls leads to difficulties in the material flow of the hot plastic melt in the injection mold and this thickening of material can also lead to material tension during sterilization.
  • Another disadvantage of such plano-convex thickenings is that the stackability of such containers deteriorates or additional shaping measures are necessary to ensure stackability despite this material thickening. The opening angle of such magnifying glasses is relatively small.
  • the invention is therefore based on the object of integrating a magnifying element into such containers for culture media using simple structural means, which allows the user to view the culture media over a large area, without having to pay for this through wall reinforcements and the disadvantages resulting therefrom becomes.
  • the transparent container section is at least partially formed by a Fresnel lens designed as a plastic part.
  • This Fresnel lens made up of a plurality of concentrically arranged grooves and ribs which are prismatic in cross-section are part of the usual wall thickness, the two planes of the Fresnel lens being able to merge plane-parallel into the adjacent wall surfaces of the container.
  • these Fresnel lenses can form circular lenses, circular ring lenses or circular segments, circular sectors or circular surface sections.
  • Injection molding, compression molding, injection compression molding or injection molding die for the actual container or parts of the container is simple to manufacture and can be used for most of the container forms on the market for culture media. Due to the flat geometry of these Fresnel lenses, the geometries provided for these containers can also be maintained with regard to stackability.
  • FIG. 1 a top view of a petri dish with a central inlet connection and with four nutrient media reservoirs arranged in the bottom
  • FIG. 2 a cross section through the petri dish according to section line 2-2 according to FIG. 1
  • FIG. 3 a top view of simple petri dish
  • FIG. 4 shows a cross section along the line 4-4 in FIG. 3
  • FIG. 5 shows a plan view of a petri dish of another
  • Embodiment and Fig. 6 is a plan view of a nutrient media holder with case.
  • the petri dish consists of a lower part 1, which is formed by four ribs 2 in four sectors for receiving four different nutrient cardboard discs 3, which are covered by a microporous membrane filter 4 with a printed grid, which micro - Retains organisms on the surface.
  • the lid 5 of the Petri dish is either clamped to the lower part 1 via a conical clamping seat or is loosely fitted with play.
  • the lid 5 has a central, closable inlet 6 for the introduction of the sample to be examined.
  • the outward-facing surface is formed as a flat Fresnel lens 7 around this central inlet 6, which consists of a plurality of concentrically arranged grooves and ribs prismatic in cross section, so that a sawtooth-like cross section in the upper part 5 arises.
  • the grid on the membrane filter 4 and the microorganisms growing on it are optically enlarged by the Fresnel lens 7.
  • the degree of magnification can be coordinated by the fineness of the sawtooth-shaped grooves and ribs of the Fresnel lens and by their distance from the surface of the cultures to be examined. Due to the large opening angle of the Fresnel lens 7, practically the entire area of the lower part 1 can be seen.
  • the lid 5 of which is rotatable relative to the lower part 1 it is sufficient to form only a part of the upper side as a Fresnel lens 7 and to leave the remaining part smooth in the usual way, so that each area of the culture is separated both by the Fresnel -Lens 7 can also be viewed without enlargement by rotating the cover 5 relative to the lower part 1.
  • a Such an embodiment is indicated schematically in FIG. 5, in which the Fresnel lens is designed as a circular lens 1 'and the other Fresnel lens as a sector 1 ' .
  • the two Fresnel lenses 7 ', 7 • • can also be designed with different magnification factors.
  • the petri dish consists of a lower part 1 'with food agar 8 and a lid 5', the upper side of which is designed overall as a Fresnel lens 7 '.
  • the embodiment according to FIG. 6 relates to a nutrient medium holder for immersion in liquid with various nutrient media which are arranged in the holder 9 and which has a handle 11.
  • Culture media can be inserted into a transparent case 10 which protects the culture media against external contamination.
  • the top of the case 10 is also equipped with a Fresnel lens 7 1 '.
  • the Fresnel lens 7 ′′ advantageously increases the growth of the various microorganisms on the various nutrient media optically.
  • the plastic Fresnel lenses are manufactured either by spraying, pressing, transfer molding or transfer molding, depending on the material and the degree of fineness of the prismatic grooves.

Landscapes

  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Un récipient, notamment une boîte de Petri utile pour effectuer des analyses qualitatives et quantitatives de cultures de cellules et de micro-organismes, comprend un couvercle transparent (5) pourvu d'une lentille en échelon de Fresnel (7) en matière plastique, à grande surface, qui permet d'observer le contenu du récipient avec un grossissement.
PCT/EP1991/001953 1990-10-17 1991-10-15 Recipient de milieux de culture et son utilisation WO1992007061A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP4032905.4 1990-10-17
DE4032905 1990-10-17

Publications (1)

Publication Number Publication Date
WO1992007061A1 true WO1992007061A1 (fr) 1992-04-30

Family

ID=6416435

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1991/001953 WO1992007061A1 (fr) 1990-10-17 1991-10-15 Recipient de milieux de culture et son utilisation

Country Status (2)

Country Link
EP (1) EP0553182A1 (fr)
WO (1) WO1992007061A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2298188A (en) * 1995-02-27 1996-08-28 David Gifford Burder Lenticular transparent box
EP1041012A1 (fr) * 1999-04-02 2000-10-04 Soremartec S.A. Emballage pourvu d'organe de grossissement en particulier pour aliments
WO2007149525A2 (fr) * 2006-06-22 2007-12-27 Manhattan Diagnostics Corp. Boîte de pétri filtrée
US20210079328A1 (en) * 2019-09-17 2021-03-18 Axogen Corporation Tissue-processing container for automated processing of tissue, methods of use thereof, and systems comprising the same

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0181075A1 (fr) * 1984-10-09 1986-05-14 Kobayashi Pharmaceutical Co. Ltd. Boîte de Pétri pour la culture de bactéries et méthode d'examen de sensibilité aux produits pharmaceutiques
EP0239058A2 (fr) * 1984-01-31 1987-09-30 Millipore Corporation Réceptacle destiné à recevoir un milieu de culture pour micro-organismes
EP0380768A2 (fr) * 1988-12-22 1990-08-08 Becton, Dickinson and Company Appareils pour subculture comprenant plusieurs milieux nutritifs solides
US4979331A (en) * 1986-11-07 1990-12-25 Sanwa Shutter Corporation Hothouse for cultivation
US4979332A (en) * 1988-07-15 1990-12-25 Kirin Beer Kabushiki Kaisha Culture vessel

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0239058A2 (fr) * 1984-01-31 1987-09-30 Millipore Corporation Réceptacle destiné à recevoir un milieu de culture pour micro-organismes
EP0181075A1 (fr) * 1984-10-09 1986-05-14 Kobayashi Pharmaceutical Co. Ltd. Boîte de Pétri pour la culture de bactéries et méthode d'examen de sensibilité aux produits pharmaceutiques
US4979331A (en) * 1986-11-07 1990-12-25 Sanwa Shutter Corporation Hothouse for cultivation
US4979332A (en) * 1988-07-15 1990-12-25 Kirin Beer Kabushiki Kaisha Culture vessel
EP0380768A2 (fr) * 1988-12-22 1990-08-08 Becton, Dickinson and Company Appareils pour subculture comprenant plusieurs milieux nutritifs solides

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2298188A (en) * 1995-02-27 1996-08-28 David Gifford Burder Lenticular transparent box
GB2298188B (en) * 1995-02-27 1998-12-02 David Gifford Burder Lenticulated transparent box
EP1041012A1 (fr) * 1999-04-02 2000-10-04 Soremartec S.A. Emballage pourvu d'organe de grossissement en particulier pour aliments
WO2007149525A2 (fr) * 2006-06-22 2007-12-27 Manhattan Diagnostics Corp. Boîte de pétri filtrée
WO2007149525A3 (fr) * 2006-06-22 2008-02-07 Manhattan Diagnostics Corp Boîte de pétri filtrée
US20210079328A1 (en) * 2019-09-17 2021-03-18 Axogen Corporation Tissue-processing container for automated processing of tissue, methods of use thereof, and systems comprising the same
US11932837B2 (en) * 2019-09-17 2024-03-19 Axogen Corporation Tissue-processing container for automated processing of tissue, methods of use thereof, and systems comprising the same

Also Published As

Publication number Publication date
EP0553182A1 (fr) 1993-08-04

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