WO1991013160A1 - Vecteurs d'expressions de cellules de mammiferes - Google Patents

Vecteurs d'expressions de cellules de mammiferes Download PDF

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Publication number
WO1991013160A1
WO1991013160A1 PCT/US1991/001078 US9101078W WO9113160A1 WO 1991013160 A1 WO1991013160 A1 WO 1991013160A1 US 9101078 W US9101078 W US 9101078W WO 9113160 A1 WO9113160 A1 WO 9113160A1
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WIPO (PCT)
Prior art keywords
plasmid
recombinant plasmid
cells
delimited
hindlll
Prior art date
Application number
PCT/US1991/001078
Other languages
English (en)
Inventor
Kevin W. Moore
Paul J. Zavodny
Original Assignee
Schering Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Schering Corporation filed Critical Schering Corporation
Publication of WO1991013160A1 publication Critical patent/WO1991013160A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5409—IL-5
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention se rapporte à de nouveaux plasmides recombinants qui peuvent être utlisés pour effectuer le clonage de gènes hétérologues dans des cellules d'E. coli et pour effectuer le clonage et l'expression de tels gènes dans des cellules de mammifères. Ces plasmides contiennent un certain nombre de sites de restriction uniques qui peuvent être utilisés pour faciliter l'élimination ou le remplacement d'éléments génétiques et l'introduction de gènes hétérologues dans les plasmides. La nature et la position de ces sites uniques sont telles que la régénération des sites après l'opération de clivage par endonucléase peut facilement être réalisée avec un minimum de manipulations. Des cellules hôtes contenant ces plasmides recombinants sont également décrites dans la présente invention.
PCT/US1991/001078 1990-02-28 1991-02-26 Vecteurs d'expressions de cellules de mammiferes WO1991013160A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48657190A 1990-02-28 1990-02-28
US486,571 1990-02-28

Publications (1)

Publication Number Publication Date
WO1991013160A1 true WO1991013160A1 (fr) 1991-09-05

Family

ID=23932407

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/001078 WO1991013160A1 (fr) 1990-02-28 1991-02-26 Vecteurs d'expressions de cellules de mammiferes

Country Status (5)

Country Link
EP (1) EP0517768A1 (fr)
JP (1) JPH05503015A (fr)
AU (1) AU7444191A (fr)
CA (1) CA2076975A1 (fr)
WO (1) WO1991013160A1 (fr)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008792A2 (fr) * 1990-11-16 1992-05-29 Schering Corporation PROCEDE D'AUGMENTATION ET D'ACTIVATION DES MONOCYTES ET DES NEUTROPHILES ET D'INDUCTION DE LA MATURATION DE CELLULES MYELOïDES A L'AIDES DE L'INTERLEUKINE-5
EP0732404A1 (fr) * 1993-12-03 1996-09-18 Asahi Kasei Kogyo Kabushiki Kaisha Nouveau vecteur de detection d'expression
EP0982398A1 (fr) * 1995-03-20 2000-03-01 The Regents Of The University Of California Acides nucléiques et leurs utilisation pour la détermination de beta-lactamase
EP1143006A2 (fr) * 1996-08-19 2001-10-10 MorphoSys AG Vecteurs/DNA-séquences provenant de banques d'anticorps humaines
WO2004070030A1 (fr) * 2003-02-03 2004-08-19 Immuno Japan Inc. Vecteur de surexpression pour une cellule animale
WO2005017148A1 (fr) 2003-07-26 2005-02-24 Trubion Pharmaceuticals, Inc. Constructions de liaison et procedes d'utilisation correspondants
US7326567B2 (en) 2003-11-12 2008-02-05 Schering Corporation Plasmid system for multigene expression
WO2010062697A2 (fr) 2008-10-30 2010-06-03 Peixuan Guo Biocapteur à connecteur de protéine motrice d'encapsulation d'adn viral à intégration membranaire pour séquençage d'adn et autres utilisations
EP2316951A1 (fr) 2001-01-17 2011-05-04 Trubion Pharmaceuticals, Inc. Proteines de fusion d'immunoglobuline de domaine de liaison
US8017735B2 (en) 2003-11-21 2011-09-13 Schering Corporation Anti-IGFR1 antibody therapeutic combinations
US8137933B2 (en) 2008-11-12 2012-03-20 Schering Corporation Mammalian expression vector pUHAB
US9017679B2 (en) 2005-08-30 2015-04-28 University Of Miami Immunomodulating tumor necrosis factor receptor 25 (TNFR25) agonists, antagonists, and immunotoxins
EP3061462A1 (fr) 2007-07-02 2016-08-31 Etubics Corporation Procédés et compositions permettant de produire un vecteur d'adénovirus destiné à être utilisé avec des vaccinations multiples
US9499627B2 (en) 2009-08-03 2016-11-22 University Of Miami Method for in vivo expansion of T regulatory cells
US9603925B2 (en) 2013-01-09 2017-03-28 University Of Miami Compositions comprising TL1A-Ig fusion protein for the regulation of T regulatory cells, and methods for their use
US9605276B2 (en) 2012-08-24 2017-03-28 Etubics Corporation Replication defective adenovirus vector in vaccination

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
EP0276730A2 (fr) * 1987-01-24 1988-08-03 BEHRINGWERKE Aktiengesellschaft Système de vecteur en forme de cassette pour l'expression d'une structure à lecture ouverte dans des cellules eucariotes

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8629153D0 (en) * 1986-12-05 1987-01-14 Ciba Geigy Ag Hybrid proteins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
EP0276730A2 (fr) * 1987-01-24 1988-08-03 BEHRINGWERKE Aktiengesellschaft Système de vecteur en forme de cassette pour l'expression d'une structure à lecture ouverte dans des cellules eucariotes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Gene, volume 71, no. 1, 15 November 1988, Elsevier Science Publishers B.V., (Amsterdam, NL), H. Ikeda et al.: "Mulcos: a vector for amplification and simultaneous expression of two foreign genes in mammalian cells ", 19-27 *
Journal of Molecular and Applied Genetics, volume 2, no. 1, 1983, Raven, (New York, US), C.V. Hall et al.: "Expression and regulation of escherichia coli lac z gene fusions in mammalian cells", pages 101-109 *
Molecular and Cellular Biology, volume 2, no. 9, 1982, American Society for Microbiology, (US), C.M. Corman et al.: "Recombinant genomes which express chloraphenicol acetyltransferase in mammalian cells" pages 1044-1051 *
Molecular and Cellular Biology, volume 8, no. 1, 1988, (US), Y. Takebe et al.: "SRx promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat", pages 466-472 *

Cited By (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008792A3 (fr) * 1990-11-16 1992-07-23 Schering Corp PROCEDE D'AUGMENTATION ET D'ACTIVATION DES MONOCYTES ET DES NEUTROPHILES ET D'INDUCTION DE LA MATURATION DE CELLULES MYELOïDES A L'AIDES DE L'INTERLEUKINE-5
WO1992008792A2 (fr) * 1990-11-16 1992-05-29 Schering Corporation PROCEDE D'AUGMENTATION ET D'ACTIVATION DES MONOCYTES ET DES NEUTROPHILES ET D'INDUCTION DE LA MATURATION DE CELLULES MYELOïDES A L'AIDES DE L'INTERLEUKINE-5
US6214613B1 (en) 1993-12-03 2001-04-10 Ashai Kasei Kogyo Kabushiki Kaisha Expression screening vector
EP0732404A1 (fr) * 1993-12-03 1996-09-18 Asahi Kasei Kogyo Kabushiki Kaisha Nouveau vecteur de detection d'expression
EP0732404A4 (fr) * 1993-12-03 1997-07-30 Asahi Chemical Ind Nouveau vecteur de detection d'expression
US6291162B1 (en) 1995-03-20 2001-09-18 The Regents Of The University Of California Cytosolic forms of beta-lactamase and uses thereof
US6472205B1 (en) 1995-03-20 2002-10-29 The Regents Of The University Of California Cytosolic forms for β-lactamase and uses thereof
EP1405922A3 (fr) * 1995-03-20 2004-09-29 The Regents of the University of California Substrats pour beta-lactamase et utilisations de ces substrats
EP0982398A1 (fr) * 1995-03-20 2000-03-01 The Regents Of The University Of California Acides nucléiques et leurs utilisation pour la détermination de beta-lactamase
US7157575B2 (en) 1995-03-20 2007-01-02 The Regents Of The University Of California Substrates for β-lactamase and uses thereof
US8071761B2 (en) 1995-03-20 2011-12-06 The Regents Of The University Of California Substrates for beta-lactamase and uses thereof
EP1143006A2 (fr) * 1996-08-19 2001-10-10 MorphoSys AG Vecteurs/DNA-séquences provenant de banques d'anticorps humaines
EP1143006A3 (fr) * 1996-08-19 2003-12-17 MorphoSys IP GmbH Vecteurs/DNA-séquences provenant de banques d'anticorps humaines
EP2316951A1 (fr) 2001-01-17 2011-05-04 Trubion Pharmaceuticals, Inc. Proteines de fusion d'immunoglobuline de domaine de liaison
EP2706116A1 (fr) 2001-01-17 2014-03-12 Emergent Product Development Seattle, LLC Proteines de fusion d'immunoglobuline de domaine de liaison
WO2004070030A1 (fr) * 2003-02-03 2004-08-19 Immuno Japan Inc. Vecteur de surexpression pour une cellule animale
WO2005017148A1 (fr) 2003-07-26 2005-02-24 Trubion Pharmaceuticals, Inc. Constructions de liaison et procedes d'utilisation correspondants
US8062886B2 (en) 2003-11-12 2011-11-22 Schering Corporation Plasmid system for multigene expression
US7326567B2 (en) 2003-11-12 2008-02-05 Schering Corporation Plasmid system for multigene expression
US8017735B2 (en) 2003-11-21 2011-09-13 Schering Corporation Anti-IGFR1 antibody therapeutic combinations
US11395846B2 (en) 2005-08-30 2022-07-26 University Of Miami Immunomodulating tumor necrosis factor receptor 25 (TNFR25) agonists, antagonists, and immunotoxins
US9017679B2 (en) 2005-08-30 2015-04-28 University Of Miami Immunomodulating tumor necrosis factor receptor 25 (TNFR25) agonists, antagonists, and immunotoxins
US9839670B2 (en) 2005-08-30 2017-12-12 University Of Miami Immunomodulating tumor necrosis factor receptor 25 (TNFR25) agonists, antagonists, and immunotoxins
EP3539567A1 (fr) 2007-07-02 2019-09-18 Etubics Corporation Procédés et compositions permettant de produire un vecteur d'adénovirus destiné à être utilisé avec des vaccinations multiples
US11376316B2 (en) 2007-07-02 2022-07-05 Etubics Corporation Breast cancer vaccines and methods of use thereof
US11826417B2 (en) 2007-07-02 2023-11-28 Etubics Corporation Methods and compositions for producing an adenovirus vector for use with multiple vaccinations
US11529408B2 (en) 2007-07-02 2022-12-20 Etubics Corporation Methods and compositions for producing an adenovirus vector for use with multiple vaccinations
US11504423B2 (en) 2007-07-02 2022-11-22 Etubics Corporation Methods and compositions for producing an adenovirus vector for use with multiple vaccinations
EP3061462A1 (fr) 2007-07-02 2016-08-31 Etubics Corporation Procédés et compositions permettant de produire un vecteur d'adénovirus destiné à être utilisé avec des vaccinations multiples
US11389521B2 (en) 2007-07-02 2022-07-19 Etubics Corporation Methods and compositions for producing an adenovirus vector for use with multiple vaccinations
US11344613B2 (en) 2007-07-02 2022-05-31 Etubics Corporation Sequential administration of a replication defective adenovirus vector in vaccination protocols
US11369673B2 (en) 2007-07-02 2022-06-28 Etubics Corporation HPV vaccines and methods of use thereof
WO2010062697A2 (fr) 2008-10-30 2010-06-03 Peixuan Guo Biocapteur à connecteur de protéine motrice d'encapsulation d'adn viral à intégration membranaire pour séquençage d'adn et autres utilisations
US9528125B2 (en) 2008-11-12 2016-12-27 Merck Sharp & Dohme Corp. Mammalian expression vector pUHAB
US8951790B2 (en) 2008-11-12 2015-02-10 Merck Sharp & Dohme Corp. Mammalian expression vector pUHAB
US8137933B2 (en) 2008-11-12 2012-03-20 Schering Corporation Mammalian expression vector pUHAB
US10934364B2 (en) 2009-08-03 2021-03-02 University Of Miami Method for in vivo expansion of T regulatory cells
US9499627B2 (en) 2009-08-03 2016-11-22 University Of Miami Method for in vivo expansion of T regulatory cells
US10563224B2 (en) 2012-08-24 2020-02-18 Etubics Corporation Replication defective adenovirus vector in vaccination
US9605276B2 (en) 2012-08-24 2017-03-28 Etubics Corporation Replication defective adenovirus vector in vaccination
USRE48599E1 (en) 2013-01-09 2021-06-22 University Of Miami Compositions comprising TLIA-Ig fusion protein for the regulation of T regulatory cells, and methods for their use
US9603925B2 (en) 2013-01-09 2017-03-28 University Of Miami Compositions comprising TL1A-Ig fusion protein for the regulation of T regulatory cells, and methods for their use

Also Published As

Publication number Publication date
CA2076975A1 (fr) 1991-08-29
EP0517768A1 (fr) 1992-12-16
AU7444191A (en) 1991-09-18
JPH05503015A (ja) 1993-05-27

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