WO1991007080A1 - Photobioreacteur - Google Patents

Photobioreacteur Download PDF

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Publication number
WO1991007080A1
WO1991007080A1 PCT/US1990/006722 US9006722W WO9107080A1 WO 1991007080 A1 WO1991007080 A1 WO 1991007080A1 US 9006722 W US9006722 W US 9006722W WO 9107080 A1 WO9107080 A1 WO 9107080A1
Authority
WO
WIPO (PCT)
Prior art keywords
light
photobioreactor
baffles
tank
set forth
Prior art date
Application number
PCT/US1990/006722
Other languages
English (en)
Inventor
Scot Douglas Hoeksema
Original Assignee
Martek Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Martek Corporation filed Critical Martek Corporation
Priority to AU69192/91A priority Critical patent/AU654659B2/en
Publication of WO1991007080A1 publication Critical patent/WO1991007080A1/fr

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/30Wastewater or sewage treatment systems using renewable energies
    • Y02W10/37Wastewater or sewage treatment systems using renewable energies using solar energy

Definitions

  • the nutrient requirements of algae are very inexpensive, carbon dioxide being the principal source of carbon.
  • the photosynthetic process requires thai, the algae be exposed to a relatively constant and uniform source of light.
  • ⁇ primary design factor for modern photobioreactors involves providing a means for uniformly exposing the cells in the algal culture to the optimum amount of visible light.
  • algae are quite sensitive to the amount and kind of light. Excessive light intensity can damage and kill algal cells. Too little light results in low levels of photosynthesis and consequently reduces growth.
  • a number of design factors are affected by the means selected for supplying light to the cells.
  • light sources including natural sunlight, often emit substantial amounts of heat.
  • ⁇ lgal cultures are sensitive to heat and many of them grow most efficiently at temperatures of 20-35°C.
  • means must often be provided for cooling the algal culture and dissipating heat generated by the light source.
  • Two design factors closely related to the requirement for a uniform and constant supply of light are the cell density and the light path length. Like conventional fermentation processes, it is usually desirable to use as high a cell density as possible.
  • An alternative embodiment of a bioreactor employing a fluorescent tube involves a cylindrical culture chamber having glass walls which surround a single fluorescent tube.
  • the culture chamber may a so be surrounded by a concentric cylindrical water jacket for controlling the temperature of the culture.
  • Such n photobioreactor is described by Radmer, R., Behrens, P., and Arnett, L., in a paper titled "An Analysis of the Productivity of a Continuous ⁇ lgal Culture System” , published in Biotechnology and Bioenqineerinq, 29 (1987), pp. 488-492.
  • This design has also proven very valuable for laboratory-scale algal culturing operations, but, for many of the reasons described above, has not proven particularly useful for large- scale operations.
  • the present invention in accordance with one embodiment thereof, comprises a novel photobioreactor in which at least one a d preferably a plurality of light transmitting baffles are mounted side by side in a tank containing a liquid microbial culture.
  • Each baffle is formed with a hollow cavity and is mounted so that the cavity is accessible from outside the. tank for the insertion of a light source.
  • the sides of the baffles are constructed of optically transparent material to transmit the light from the light source to the liquid which is in contact with the outside surfaces of the baffles.
  • Each light source is made up of a plurality of light tubes, preferably fluorescent lamps, supported by braces or similar supporting structures and mounted in the baffles. Electrical leads are extended from the tubes to allow connection with an external power source.
  • a single high intensity light source is mounted in a light compartment having walls made of internally reflective prismatic sheet material to provide uniform liglit of a suitable intensity to the microbial liquid culture.
  • the invention thus provides for greatly simplified electrical circuitry and connections, and reduces maintenance costs. Enabling access to the light sources from outside the tank and shielding the liglit sources from actual contact with the microbial culture makes it easier to identify and replace burned bulbs and it reduces the risks of short circuits as well. Furthermore, as the light transmitting baffles are surrounded on its major light emitting surfaces by the liquid microbial culture, the spacing between adjacent tubes as well as between adjacent baffles is such as to optimize absorption of the emitted light by the algae and to assure virtually complete absorption of the emitted light.
  • the light transmitting baffles also perform a structural function in that their external surfaces serve as walls or draft spaces to define circulation paths through which the algae is moved by means such as air lift agitation.
  • the baffles also form basic building blocks or modules which can be used in combination in any desired number for large scale photobioreactor systems of any selected capacity.
  • Figure 1 is a perspective view of the photobioreactor illustrating an embodiment of the present invention
  • Figure 2 is perspective view of a baffle and fluorescent tube arrangement forming part of the photobioreactor of Figure 1;
  • Figures 3-5 are top, side, and bottom views respectively of the photobioreactor illustrating the present invention.
  • Figure 6 is a perspective view of an alternate light source for the photobioreactor illustrating another embodiment of the present invention
  • Figure 7 is a perspective view of the photobioreactor illustrating the present invention utilizing the light source of Figure 6.
  • Figure 1 is a perspective view of a photobioreactor 10 embodying the present invention to be used in the culturing of dispersed cells or cell aggregates or multicellular organisms having a ligh requirement. ⁇ s an example, this photobioreactor may be used to grow unicellular algae which carry on photosynthesis.
  • the exterior of the photobioreactor 10 is in the form of a tank 11 capable of containing a liquid culture medium as illustrated by numeral 12.
  • the liquid culture medium is sometimes referred to as an "algal" culture, but it will be appreciated that the photobioreactor 10 may be employed for the cultivation of any type of photosynthetic microorganism.
  • the basic unit of the photobioreactor is a rectangular tank 11 as shown in Figure 1 with numerous internal baffles 14 which extend from one end of the tank to the other, and whose ends are sealed to the tank's inside walls.
  • Each baffle 14 is formed with a hollow cavity which is accessible through openings in the wall of the tank.
  • Tank end walls 13 can be cut out or molded in any conventional manner to enable access to the baffle cavities from outside the tank's end wall surface.
  • a plurality of light tubes 15 are inserted into the baffle cavity from outside the tank's surface and housed therein.
  • Baffles 14 serve to protect light tubes 15 from direct contact with the liquid culture medium 12. Light is therefore emitted substantially uniformally from tubes 15 and is absorbed by the algal culture.
  • FIG. 1 shows a rectangularly shaped tank 11 , it is recognized that any convenient shape may be used.
  • the tank and baffle structure of the present invention is an improvement over the photobioreactor disclosed in pending in application S.N. 07/163,841, incorporated herein by reference, which discloses a compartment for protecting a light bank against fluid communication with a liquid culture medium. Permitting insertion of a light source into the baffle cavities through the tank's outer surfaces, as in the present invention, greatly facilitates electrical connectors to the light source and maintenance as well.
  • Located outside the tank 11 is means to control the temperature of the contents .
  • a preferred means or controlling the temperature include water jackets or internal heat transfer coils which can be connected to refrigeration units (not shown) or heating units (not shown). Also, the dissolved oxygen and pH levels of the contents are continuously monitored and controlled by any conventional, well known means.
  • FIG 2 is a perspective view of a baffle 14 and the housing of light tubes 15 therein.
  • Side panels 17 are substantially planar walls forming the baffle cavity therebetween. Planar walls 17 are major surfaces on the opposite sides of the cavity for the emission of light into said cavity.
  • Side panels 17, top panel 18 and bottom panel 19 are made of a chemically inert and optically transparent material such as glass or acrylic.
  • Internal braces 16 are mounted to facilitate placement and to support light tubes 15 or a bank of light tubes.
  • Open baffle ends 20 permit the simple insertion of tubes through either end even while the photobioreactor is in an operational state as shown in Figure 1.
  • light tubes 15 are fluorescent tube lamps essentially in their "off the shelf” condition without any modification or customization.
  • the advantages of such lamps in this embodiment are that light is emitted from them substantially uniformly along the length of the tubes and in all directions perpendicular to the tubes.
  • end sections 20 of baffle 14 are open, electrical connections can easily and safely be made to light tubes 15 at their opposite ends in any conventional, well known way.
  • individual connections are made to each fluorescent tube 15 by members 22 whereby leads are brought out through wires 23 and 24 which terminate in an electrical plug connectable to a source of electric power (not shown).
  • Suitable ballasts are provided as well.
  • These connections could also be made by providing a single adapter on each end section of the baffle or by grouping the tubes in any number.
  • only one end of the baffle cavity is opened to the tank's outside surface. This structure would require running the lead wires connecting the fluorescent tubes at the closed end through the baffle cavity to the open end.
  • baffles 14 are constructed to be 2 inches wide to enable the insertion of a fluorescent tube.
  • the overall height of the baffles is determined by the number of fluorescent tubes to be inserted. In this embodiment, the height is approximately 24 inches which, as shown in Figure 2, allows for 12 fluorescent tubes and three braces 16.
  • the outer surfaces of adjacent baffles are separated by a distance of 1 inch, and one-half inch separates a baffle's outer surface and the tank wall.
  • Baffles 14 will generally extend along the entire 48 inch tank length as illustrated in Figure 5.
  • the overall height and width of the tank in this embodiment are 31 inches and 12 inches as shown in Figures 4 and 3 respectively. Using high, output, cool white fluorescent tubes, this spacing provides a near optimal light source for micro algae.
  • planar walls 17 form a major portion of the light emitting surfaces of the baffle.
  • approximately 90% of the light emitted by the fluorescent tubes 15 is transmitted through planar walls 17.
  • the particular tank and baffle dimensions shown in Figures 3-5 are for illustrative purposes only. It is to be recognized that the baffle widths and spacing between adjacent baffles will depend upon the photon flux of the light source, the optical properties of the baffle walls and the cell density of the culture being used. Furthermore, the photobioreactor can easily be constructed in any volume due to the tank's internal symmetry of the light-transmitting baffles.
  • the photobioreactor in Figure 1 has a working volume of 110-130 liters. This volume can easily be increased by widening the tank, increasing the height and increasing the number of baffles. Such a change in the photobioreactor design does not affect the operation or performance of the photobioreactor in any way.
  • the light transmitting baffles also perform a structural function in that their external surfaces serve as walls or draft spaces to define circulation paths for the algal culture.
  • baffles 14 are mounted in the tank to form passages 21 therebetween to enable the culture circulation and to enhance the growth process.
  • a series of hollow tubes or cylinders 20, preferably formed of a metal or ceramic material or inert material, are placed, in the preferred embodiment, between alternate pairs of baffles as illustrated in Figure 1.
  • the cylinders 20 can also be positioned between any adjacent baffles at any vertical, position or between the baffle and the tank wall.
  • cylinders 20 can be placed just below the baffles such that substantially all the gas flows there-between as illustrated in Figure 7.
  • the cylinders 20 contain small perforations or apertures extending through the walls thereof forming gas sparging tubes through which a pressurized gas (e.g. carbon dioxide) is supplied for the photosynthesis requirements of the algal culture.
  • a pressurized gas e.g. carbon dioxide
  • the gas may be air or nitrogen or another inert gas either singly or enriched with carbon dioxide-.
  • Other means for supplying nutrient source gases and for circulating the culture may also be used.
  • baffles extend through the entire length of the tank and the hollow cavities formed therein are accessible from the outer surface of the tank walls on either side, supplying electrical connections to the fluorescent tubes housed therein is greatly simplified. Providing maintenance for the tubes is simplified as well. Electrical leads connected to the ends of the tubes are made in the simple conventional manner and are completely shielded from the liquid culture 12. Recognition and replacement of burned light tubes is greatly facilitated by this novel structure. Furthermore, by selecting the proper light path lengths as described above, virtually 100% of the emitted light is absorbed by the culture and the light absorption is relatively uniform and optimized throughout the culture as well.
  • the light source is a single concentrated high-intensity light 31 mounted in a light compartment 30 to provide uniform high intensity light to the microbial liquid culture.
  • Light compartment 30 contains means for substantially uniformally distributing liglit from source 31 with reflector 33 across the interior surface of transparent, walls 34 and 35.
  • Such means maybe in the form of a light guide constructed of internally reflective prismatic sheet material (not shown).
  • the internally reflective prismatic sheet material is formed from highly transparent flexible sheet material, such as polyacrylate, on the surface of which is inscribed with minute 90° corrugations. As a result of these corrugations, light striking the sheet with an angle of incidence of about 27° or less will be reflected with nearly 100% efficiency. Minor imperfections in the prismatic sheets as well as light infringing the sheets at angles greater than about 27° result in transmission of light through the prismatic sheet material.
  • a mirror 32 is located at the bottom of light compartment 30.
  • the internal reflectance of the prismatic sheet material, reflectance of mirror 32 and reflector 33 light from the light source 31 is, to a large extent, reflected back into the compartment.
  • the net result of this internal reflectance is that the light from the light source is distributed substantially uniformly across the inner surface of wall 33 and thus provides a highly controlled distribution of light throughout the light paths in the walls 34 and 35. Light from the light source is therefore emitted and distributed substantially uniformally from the exterior surfaces of the baffle's planar walls.
  • a reflective cover (not shown) is placed between the compartment wall and the internally reflective prismatic sheet so that the light is redirected back into the compartment.
  • Mirrors may be used as end surfaces 36 and 37 as well.
  • light source 31 could be placed near end surfaces 36 and 37 in vertical arrangement to effect the uniform emittance. Constructing a light compartment utilizing a prismatic sheet material and the light guides formed therefrom is described in U.S. Patent Application S.N. 07/338,532, incorporated herein by reference.
  • the electrical power can be supplied to the high intensity light source 31 in any conventional manner. As illustrated in this embodiment, leads connected to source 31 are brought out through wire 38 and terminate in an electrical plug connectable to a source of electric power (not shown).
  • FIG. 7 shows a perspective view of the photobioreactor utilizing the light source 30.
  • Photobioreactor 40 operates in an identical way as described with reference to photobioreactor 10.
  • Baffles 41 are formed with hollow cavities extending the entire tank length and are constructed to enable the insertion of the light source from the either or both ends of the tank, through open ends 42. Electrical connections to source 30 are very simply made in any well known, conventional way as described above.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Photoreceptors In Electrophotography (AREA)
  • Glass Compositions (AREA)

Abstract

Un photobioréacteur de culture de microorganismes photosynthétiques comprend une pluralité de chicanes montées dans la cuve du photobioréacteur de façon à former des cavités creuses, ce qui permet d'insérer des sources de lumière à travers des ouvertures ménagées dans la paroi de la cuve. Les chicanes protègent les sources de lumière qui y sont logées de la culture photosynthétique liquide contenue dans la cuve, ce qui permet d'établir plus facilement des connexions électriques avec les sources de lumière et de les entretenir.
PCT/US1990/006722 1989-11-22 1990-11-16 Photobioreacteur WO1991007080A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU69192/91A AU654659B2 (en) 1989-11-22 1990-11-16 Photobioreactor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44008489A 1989-11-22 1989-11-22
US440,084 1989-11-22

Publications (1)

Publication Number Publication Date
WO1991007080A1 true WO1991007080A1 (fr) 1991-05-30

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ID=23747369

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/006722 WO1991007080A1 (fr) 1989-11-22 1990-11-16 Photobioreacteur

Country Status (6)

Country Link
EP (1) EP0502118A4 (fr)
JP (1) JPH05501649A (fr)
AU (1) AU654659B2 (fr)
CA (1) CA2069414A1 (fr)
IL (1) IL96437A (fr)
WO (1) WO1991007080A1 (fr)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2678946A1 (fr) * 1991-07-12 1993-01-15 Ovi Photoreacteur pour la culture en masse de microorganismes en conditions photocontrolees.
FR2698350A1 (fr) * 1992-11-23 1994-05-27 Commissariat Energie Atomique Dispositif d'épuration d'un effluent liquide chargé en polluants et procédé d'épuration de cet effluent.
WO2005059087A1 (fr) * 2003-12-16 2005-06-30 Inha-Industry Partnership Institute Photobioreacteur multicouches et procede de culture de micro-organismes photosynthetiques faisant intervenir ce photobioreacteur
NL1027743C2 (nl) * 2004-12-14 2006-06-16 Jan Marinus Van Eijk Bio-algenfilter.
EP2228432A1 (fr) * 2009-03-08 2010-09-15 SSC Strategic Science Consult GmbH Bioréacteur et procédé destiné au fonctionnement d'un bioréacteur
ES2377619A1 (es) * 2007-11-28 2012-03-29 Inha-Industry Partnership Institute Fotobioreactor para el cultivo a gran escala de microalgas.
CN101643699B (zh) * 2009-06-22 2012-07-04 新奥科技发展有限公司 一种利用废水养殖微藻的系统
WO2012098031A1 (fr) * 2011-01-17 2012-07-26 Wacker Chemie Ag Photobioréacteur éclairé au moyen de pièces moulées luminescentes
WO2013132117A1 (fr) * 2012-03-06 2013-09-12 Idesa, Ingenieria Y Diseño Europeo S.A. Photobioréacteur à système rotatif d'homogénéisation et d'éclairage intérieur incorporé
WO2014176852A1 (fr) * 2013-04-28 2014-11-06 Zhong Qi Dispositif de culture de micro-organismes
CN106675992A (zh) * 2017-01-17 2017-05-17 杭州元点生物科技有限公司 一种控制微藻培养循环的搅拌系统

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102019007167A1 (de) * 2019-10-15 2021-04-15 Hochschule Kaiserslautern Emerser Bioreaktor

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU505405A1 (ru) * 1973-04-19 1976-03-05 Предприятие П/Я А-7555 Установка дл производства водорослей
US4253418A (en) * 1978-08-23 1981-03-03 Monterey Abalone Farms Abalone mariculture
US4555864A (en) * 1983-06-24 1985-12-03 Kei Mori Chlorella nurturing device
US4626065A (en) * 1981-12-03 1986-12-02 Kei Mori Light conduction apparatus utilizing intermittent illumination applied to optical fibers for photosynthetic reaction
US4676956A (en) * 1982-12-24 1987-06-30 Kei Mori Apparatus for photosynthesis
US4724214A (en) * 1982-01-16 1988-02-09 Kei Mori Apparatus for photosynthesis
US4900678A (en) * 1981-12-03 1990-02-13 Kei Mori Apparatus for photosynthesis

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU505405A1 (ru) * 1973-04-19 1976-03-05 Предприятие П/Я А-7555 Установка дл производства водорослей
US4253418A (en) * 1978-08-23 1981-03-03 Monterey Abalone Farms Abalone mariculture
US4626065A (en) * 1981-12-03 1986-12-02 Kei Mori Light conduction apparatus utilizing intermittent illumination applied to optical fibers for photosynthetic reaction
US4900678A (en) * 1981-12-03 1990-02-13 Kei Mori Apparatus for photosynthesis
US4724214A (en) * 1982-01-16 1988-02-09 Kei Mori Apparatus for photosynthesis
US4676956A (en) * 1982-12-24 1987-06-30 Kei Mori Apparatus for photosynthesis
US4555864A (en) * 1983-06-24 1985-12-03 Kei Mori Chlorella nurturing device

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2678946A1 (fr) * 1991-07-12 1993-01-15 Ovi Photoreacteur pour la culture en masse de microorganismes en conditions photocontrolees.
FR2698350A1 (fr) * 1992-11-23 1994-05-27 Commissariat Energie Atomique Dispositif d'épuration d'un effluent liquide chargé en polluants et procédé d'épuration de cet effluent.
EP0599711A1 (fr) * 1992-11-23 1994-06-01 Commissariat A L'energie Atomique Dispositif d'épuration d'un effluent liquide chargé en polluants et procédé d'épuration de cet effluent
US5447629A (en) * 1992-11-23 1995-09-05 Commissariat A L'energie Atomique Apparatus for purifying a liquid effluent containing pollutants
CN1316004C (zh) * 2003-12-16 2007-05-16 仁菏大学校产学协力团 多层光照生物反应器及用其培养光合成微生物的方法
WO2005059087A1 (fr) * 2003-12-16 2005-06-30 Inha-Industry Partnership Institute Photobioreacteur multicouches et procede de culture de micro-organismes photosynthetiques faisant intervenir ce photobioreacteur
NL1027743C2 (nl) * 2004-12-14 2006-06-16 Jan Marinus Van Eijk Bio-algenfilter.
ES2377619A1 (es) * 2007-11-28 2012-03-29 Inha-Industry Partnership Institute Fotobioreactor para el cultivo a gran escala de microalgas.
EP2228432A1 (fr) * 2009-03-08 2010-09-15 SSC Strategic Science Consult GmbH Bioréacteur et procédé destiné au fonctionnement d'un bioréacteur
CN101643699B (zh) * 2009-06-22 2012-07-04 新奥科技发展有限公司 一种利用废水养殖微藻的系统
WO2012098031A1 (fr) * 2011-01-17 2012-07-26 Wacker Chemie Ag Photobioréacteur éclairé au moyen de pièces moulées luminescentes
WO2013132117A1 (fr) * 2012-03-06 2013-09-12 Idesa, Ingenieria Y Diseño Europeo S.A. Photobioréacteur à système rotatif d'homogénéisation et d'éclairage intérieur incorporé
WO2014176852A1 (fr) * 2013-04-28 2014-11-06 Zhong Qi Dispositif de culture de micro-organismes
CN106675992A (zh) * 2017-01-17 2017-05-17 杭州元点生物科技有限公司 一种控制微藻培养循环的搅拌系统

Also Published As

Publication number Publication date
EP0502118A4 (en) 1993-01-13
JPH05501649A (ja) 1993-04-02
IL96437A (en) 1994-04-12
AU654659B2 (en) 1994-11-17
EP0502118A1 (fr) 1992-09-09
IL96437A0 (en) 1991-08-16
CA2069414A1 (fr) 1991-05-23
AU6919291A (en) 1991-06-13

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