WO2015126002A1 - Bioréacteur trapézoïdal à fluorescence polarisée - Google Patents

Bioréacteur trapézoïdal à fluorescence polarisée Download PDF

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Publication number
WO2015126002A1
WO2015126002A1 PCT/KR2014/003349 KR2014003349W WO2015126002A1 WO 2015126002 A1 WO2015126002 A1 WO 2015126002A1 KR 2014003349 W KR2014003349 W KR 2014003349W WO 2015126002 A1 WO2015126002 A1 WO 2015126002A1
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WIPO (PCT)
Prior art keywords
trapezoidal
photobioreactor
housing
gas
microalgae
Prior art date
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PCT/KR2014/003349
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English (en)
Korean (ko)
Inventor
양지원
권종희
김선민
김교찬
이한솔
남기복
정주영
김웅
Original Assignee
재단법인 탄소순환형 차세대 바이오매스 생산전환 기술연구단
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Publication of WO2015126002A1 publication Critical patent/WO2015126002A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/02—Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus

Definitions

  • the present invention relates to a planar photobioreactor (PBR) having a trapezoidal shape that inclines and tilts the wall structure of the photobioreactor.
  • the surface of the inclined reactor is attached to the incubator surface by microalgae.
  • a tacky biofilm is formed and severe biofouling occurs on the wall, reducing light transmittance and thus preventing cell growth. Therefore, in the present invention, the bubble flowing from the lowest portion of the inside of the reactor for aeration inside the PBR is efficiently circulated ascending and rising up the surface of the inclined reactor.
  • the bubble prevents microorganisms from tangling or coagulating by inhibiting biofilm or biofouling on the wall, thereby providing a flat panel bioreactor capable of increasing cell growth rate and growth rate.
  • Photobioreactor is a closed culture system, such as tube type, flat type, spiral, stirred tank and hybrid type photobioreactor.
  • the performance of PBR is greatly influenced by culture strain, light, culture density, CO2 concentration, O2 accumulation, pH, and temperature.
  • the cultivation of algae using PBR is too expensive to manufacture and energy balance as well as production cost is an important factor.
  • the problem of culturing algae in open lakes is that open cultivation is difficult to adopt because lakes can be easily contaminated and eroded by invasive species. Therefore, most algal cultures use photobioreactors that choose how to grow algae while controlling light, carbon dioxide and water in closed spaces.
  • Korean Patent Registration No. 10-0439971 bubble tower photobioreactor and a method of culturing photosynthetic microorganisms using the same
  • a transparent outer column having a vertical cylindrical shape to form a chamber for containing microbial culture liquid
  • a light emitter installed at the center of the outer column to irradiate light energy to the front surface of the culture medium without disturbing the circulation of the culture medium
  • a vertical cylindrical transparent jacket separating the light emitter from the culture medium and allowing heat exchange
  • a baffle plate having a transparent plate shape installed on an outer surface of the transparent jacket to form a circulation path of the culture solution
  • an aeration device for causing upward flow of the culture liquid by supplying gas upward from a lower end of one portion divided by the baffle plate, and a method for culturing the photosynthetic microorganism using the same.
  • Patent Publication No. 10-2013-0006409 (Photobioreactor and microalgae cultivation method and harvesting method using the same) supplies a gas source containing photosynthetic carbon source directly to the culture tank to evenly distribute the microalgae by simply aeration without a separate stirring means. By dispersing to facilitate the use of light can produce microalgae of high concentration culture.
  • Korean Patent Publication No. 10-2013-0006409 (Photobioreactor and microalgae cultivation method and harvesting method using the same) supplies a gas source containing photosynthetic carbon source directly to the culture tank to evenly distribute the microalgae by simply aeration without a separate stirring means. By dispersing to facilitate the use of light can produce microalgae of high concentration culture.
  • 10-2011-0024732 (Photo-bioreactor having a wavelength conversion layer) is a wavelength conversion layer for converting the wavelength of light and the reaction vessel provided with an inlet and outlet for the introduction and discharge of microalgae and culture medium (wave) -length conversion layer) and provides a microalgae culture photobioreactor comprising a wavelength conversion unit attached to the reaction vessel. Increasing the photosynthetic efficiency of the microalgae, and may also increase the culture of the microalgae.
  • 10-0283026 (photobioreactor) is an outer jacket for forming a chamber that can contain the microbial culture fluid;
  • a vertical cylindrical transparent inner conduit installed in the outer jacket and having an upper and lower ends open to form a circulation path of the culture solution;
  • An aeration device which communicates with the lower end of the inner conduit and upwardly supplies air or other gas to cause upward flow of the culture solution in the inner conduit;
  • one or more light emitters installed on the inner surface or the outer surface of the inner conduit so as not to interfere with the circulation of the culture solution and irradiating light energy to the front surface of the culture solution.
  • 10-2011-0098622 photobioreactor having a spiral light source
  • a reaction vessel for culturing microalgae an axis installed perpendicular to the center of the reaction vessel, and a light source installed on the axis
  • microalgae are cultivated, microalgae are effectively provided with light necessary for photosynthesis, microalgae in the reaction vessel are uniformly provided, and the rotation axis is rotated so that the carbon dioxide and the culture medium are mixed more efficiently. can do.
  • 10-2013-0101692 (vinyl sheet-type photobioreactor and its manufacturing method) is a vertical arrangement of a large number of dogs can be cultivated photosynthetic microorganisms and can increase the light transmittance of the vinyl sheet-type photobioreactor and its production
  • a culture space for accommodating photosynthetic microorganisms and culture medium is formed therein, and the front and rear surfaces are bonded by heat bonding to form a plurality of permeation portions of the vinyl material and carbon dioxide inside the reaction sheet
  • 10-1290256 (microalgae automatic feed aquarium using a flat fluorescent bioreactor) is a water tank with a space for accommodating aquatic life, and an oxygen supply unit for supplying oxygen to the space
  • the culture space is provided inside so that the microalgae can be cultured therein, the plate-shaped reaction vessels are installed spaced apart from each other at predetermined intervals, and the optical reaction vessel It is provided with a culture unit provided with a light irradiation unit for irradiating, and a food supply unit for supplying the microalgae cultured from the culture unit to the habitat space.
  • Photo-bioreactor for mass culture of microalgae is a plate-shaped light source that is applied to one side and the other side of a plate-shaped substrate by applying LED, OLED, and flexible LED lamp, and applied to the photo-bioreactor. It features a photobioreactor for mass production of microalgae that not only requires efficient transmission of light, but also requires no additional equipment and investment for scale up and down.
  • the present invention is a trapezoidal PBR that is configured differently from conventional PBR such as cylindrical, conical, spiral, etc., and its technical configuration is different from those of the prior art.
  • microalgae form biofilm on the surface of the incubator, preventing the efficient transmission of light, and entangle each other to reduce the accessibility of nutrients in the culture medium. Interfering with this was a major problem for the culture.
  • the formation of biofilms formed by microorganisms by using a physical method through direct contact
  • the air bubbles are swept up the wall by spraying air bubbles at regular intervals on both planar corners of the bottom of the bottom of the reactor.
  • the rising force of the air bubbles rise up the wall and fall off the central part, creating an effective circulation, allowing microalgae to receive light, nutrients, and CO2 evenly.
  • the incubator can be used to cultivate outdoors using the incubator, so that it can receive sunlight more effectively than the incubator having a vertical wall so that the light energy can be used more efficiently.
  • the PBR of the present invention reduces light transmission due to the sticky biofilm attached to the surface of the reactor inclined by the microalgae and biofouling generated on the wall to hinder cell growth. By suppressing biofilm or biofouling on the wall, microorganisms can be prevented from tangling and coagulation, thereby providing a flat fluorescent bioreactor capable of increasing cell growth rate and growth rate.
  • 1 is a trapezoidal plate type photobioreactor of the present invention.
  • Figure 2 is a view showing the flow of air in the trapezoidal plate-type photobioreactor of the present invention (sectional view).
  • Trapezoidal plate-type photobioreactor of the present invention is 1; Photobioreactor, 11; Sparger, 12: gas outlet, 21; Inlet, 22; Gas inlet, 23; It consists of a medium outlet. Gas is introduced into the medium inside the incubator through the sparger, which is the gas supply port 11 at the bottom, by using a physical method through direct contact with the wall, and bubbles are generated at both flat corners. By spraying the air bubbles at regular intervals, the air bubbles swept up the wall, essentially suppressing the formation of the biofilm formed by the microorganisms.
  • the incubator of the present invention was inoculated into the medium using a microalga Chlamydomonas reinhardtii , and gas was supplied to obtain a microalgae.
  • microalgae was carried out as in Example 1 except that Chlorella protothecoides were used.
  • the microalgae was carried out as in Example 1 except that Chlorella vulgaris was used.
  • Microalgae were cultured using the same strain and medium as in Example 1 using a general photobioreactor.
  • the gas introduced through the gas inlet 22 is supplied to the medium inside the incubator through a sparger, which is a gas supply port 11 at the bottom, and bubbles are sprayed at regular intervals from both flat corners, thereby allowing air bubbles to wall.
  • a sparger which is a gas supply port 11 at the bottom

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un bioréacteur trapézoïdal à fluorescence polarisée. Plus particulièrement, la formation d'un biofilm, qui est formé par un micro-organisme photosynthétique, est essentiellement inhibée par la pulvérisation de bulles d'air à certains intervalles au niveau des deux bords plats du fond de la partie plus basse du réacteur pour que les bulles d'air montent sur les surfaces de paroi. De plus, un milieu de culture monte le long des surfaces de paroi grâce à l'ascension des bulles d'air et tombe au niveau d'une partie centrale de sorte qu'une circulation efficace est obtenue, permettant à la lumière, aux nutriments, et au CO2 d'alimenter uniformément les micro-algues. En outre, grâce aux parois inclinées, lorsque la culture est effectuée en extérieur au moyen du réacteur, il est possible de recevoir la lumière solaire plus efficacement que lorsqu'on utilise un réacteur à parois verticales, ce qui permet une utilisation plus efficace de l'énergie lumineuse.
PCT/KR2014/003349 2014-02-18 2014-04-17 Bioréacteur trapézoïdal à fluorescence polarisée WO2015126002A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2014-0018631 2014-02-18
KR1020140018631A KR20150097296A (ko) 2014-02-18 2014-02-18 사다리꼴 평판형광생물반응기

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018096107A1 (fr) * 2016-11-25 2018-05-31 Centre National De La Recherche Scientifique (Cnrs) Module pour photobioreacteur et photobioreacteur associe
CN115336552A (zh) * 2022-07-14 2022-11-15 山东中林东平湖发展有限公司 一种绿色低碳的鱼塘养殖供氧系统
WO2023073454A1 (fr) * 2021-10-29 2023-05-04 Bluemater, S.A. Photobioréacteur pour la culture de macro ou de micro-organismes, l'évaporation liquide ou la fermentation liquide

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7905049B2 (en) * 2007-11-01 2011-03-15 Independence Bio-Products, Inc. Algae production
US20110159581A1 (en) * 2009-12-30 2011-06-30 Tongji University Photobioreactor for carbon dioxide mitigation in wastewater treatment
CN102746982A (zh) * 2011-04-18 2012-10-24 王淋淋 多层次可抑制微藻附壁生长的大容量箱式光生物反应器
KR20130006409A (ko) * 2012-12-21 2013-01-16 한국생명공학연구원 광생물반응기와 이를 이용한 미세조류 배양법 및 수확법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7905049B2 (en) * 2007-11-01 2011-03-15 Independence Bio-Products, Inc. Algae production
US20110159581A1 (en) * 2009-12-30 2011-06-30 Tongji University Photobioreactor for carbon dioxide mitigation in wastewater treatment
CN102746982A (zh) * 2011-04-18 2012-10-24 王淋淋 多层次可抑制微藻附壁生长的大容量箱式光生物反应器
KR20130006409A (ko) * 2012-12-21 2013-01-16 한국생명공학연구원 광생물반응기와 이를 이용한 미세조류 배양법 및 수확법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHUANG, LIN-LAN ET AL.: "A novel suspended-solid phase photobioreactor to improve biomass production and separation of microalgae", BIORESOUR. TECHNOL., vol. 153, no. 2014, 15 December 2013 (2013-12-15), pages 399 - 402, XP028669188, ISSN: 0960-8524 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018096107A1 (fr) * 2016-11-25 2018-05-31 Centre National De La Recherche Scientifique (Cnrs) Module pour photobioreacteur et photobioreacteur associe
FR3059335A1 (fr) * 2016-11-25 2018-06-01 Centre National De La Recherche Scientifique (Cnrs) Module pour photobioreacteur et photobioreacteur associe
WO2023073454A1 (fr) * 2021-10-29 2023-05-04 Bluemater, S.A. Photobioréacteur pour la culture de macro ou de micro-organismes, l'évaporation liquide ou la fermentation liquide
CN115336552A (zh) * 2022-07-14 2022-11-15 山东中林东平湖发展有限公司 一种绿色低碳的鱼塘养殖供氧系统

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