WO1991002798A3 - Gk1l mutante d'activateur tissulaire du plasminogene - Google Patents

Gk1l mutante d'activateur tissulaire du plasminogene Download PDF

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Publication number
WO1991002798A3
WO1991002798A3 PCT/EP1990/001401 EP9001401W WO9102798A3 WO 1991002798 A3 WO1991002798 A3 WO 1991002798A3 EP 9001401 W EP9001401 W EP 9001401W WO 9102798 A3 WO9102798 A3 WO 9102798A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant dna
domains
protein
vectors
gene
Prior art date
Application number
PCT/EP1990/001401
Other languages
German (de)
English (en)
Other versions
WO1991002798A2 (fr
Inventor
Ulrich Weidle
Anne Stern
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Priority to DE59008764T priority Critical patent/DE59008764D1/de
Priority to EP90912217A priority patent/EP0440763B1/fr
Publication of WO1991002798A2 publication Critical patent/WO1991002798A2/fr
Publication of WO1991002798A3 publication Critical patent/WO1991002798A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Oscillators With Electromechanical Resonators (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Curing Cements, Concrete, And Artificial Stone (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

ADN de recombinaison codant pour une protéine comportant les domaines G, K1 et L de t-PA (activateur tissulaire du plasminogène), dans laquelle les séquences codant pour les domaines K2 et F du gène de t-PA de type sauvage ou les séquences dérivées de celui-ci dans le contexte du code génétique, sont complètement supprimées en fonction des limites d'exons/introns exactes se trouvant dans le gène de t-PA. Procédés de préparation dudit ADN de recombinaison. Vecteurs contenant ledit ADN de recombinaison, et cellules transformées à l'aide desdits vecteurs ou dudit ADN de recombinaison. Protéine présentant des propriétés fibrinolytiques par expression d'une séquence d'ADN de l'invention dans des cellules hôtes appropriées, laquelle est composée des séquences d'acides aminés des domaines G, K1 et L de t-PA se trouvant dans cette séquence, et peut être glycosylée. Procédé de préparation de ladite protéine. Agent fibrinolytique contenant une protéine obtenue selon l'invention.
PCT/EP1990/001401 1989-08-23 1990-08-22 Gk1l mutante d'activateur tissulaire du plasminogene WO1991002798A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE59008764T DE59008764D1 (de) 1989-08-23 1990-08-22 t-PA-MUTANTE GK1L.
EP90912217A EP0440763B1 (fr) 1989-08-23 1990-08-22 Gk1l mutante d'activateur tissulaire du plasminogene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3927865A DE3927865A1 (de) 1989-08-23 1989-08-23 T-pa-mutante gk1l
DEP3927865.4 1989-08-23

Publications (2)

Publication Number Publication Date
WO1991002798A2 WO1991002798A2 (fr) 1991-03-07
WO1991002798A3 true WO1991002798A3 (fr) 1991-04-04

Family

ID=6387718

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1990/001401 WO1991002798A2 (fr) 1989-08-23 1990-08-22 Gk1l mutante d'activateur tissulaire du plasminogene

Country Status (6)

Country Link
US (1) US5223422A (fr)
EP (1) EP0440763B1 (fr)
JP (1) JPH04500010A (fr)
AT (1) ATE120236T1 (fr)
DE (2) DE3927865A1 (fr)
WO (1) WO1991002798A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2000268170A1 (en) * 2000-09-04 2002-03-22 Hunan Royal Biotech A cell line expressing mutant human tissue-type plasminogen activator, constructing strategy and method of expressed protein preparation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987003906A1 (fr) * 1985-12-20 1987-07-02 The Upjohn Company Analogues d'un activateur de plasminogene tissulaire (tpa)

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987003906A1 (fr) * 1985-12-20 1987-07-02 The Upjohn Company Analogues d'un activateur de plasminogene tissulaire (tpa)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Febs Letters, Band 189, Nr. 1, September 1985, Published by Elsevier Science Publishers B.V. (Biomedical Division), Federation of European Biochemical Societies, (Amsterdam, NL), H. KAGITANI et al.: "Expression in E. Coli of Finger-Domain Lacking Tissue-Type Plasminogen Activator with High Fibrin Affinity" seiten 145-149, siehe den ganzen artikel *
Gene, Band 79, Nr. 2, 1989, Elsevier Science Publishers B.V. (Biomedical Division), (Amsterdam, NL), A. STERN et al.: "Functional Topology of Human Tissue-Type Plasminogen Activator: Characterization of two Deletion Derivatives and of a Duplication Derivative", seiten 233-344, siehe den ganze artikel *
Gene, Band 87, Nr. 2, 15. Marz 1990, Elsevier Science Publishers B.V. (Biomedical Division), (Amsterdam, NL), A. STERN et al.: "Kringle 1 Domain of Human Tissue-Type Plasminogen Activator is a Functional Module Mediating Fibrinogen-Stimulated Plasminogenolytic Activity", seiten 305-308, siehe den ganze artikel *

Also Published As

Publication number Publication date
DE3927865A1 (de) 1991-02-28
JPH04500010A (ja) 1992-01-09
ATE120236T1 (de) 1995-04-15
DE59008764D1 (de) 1995-04-27
EP0440763A1 (fr) 1991-08-14
WO1991002798A2 (fr) 1991-03-07
EP0440763B1 (fr) 1995-03-22
US5223422A (en) 1993-06-29
JPH0571228B2 (fr) 1993-10-06

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