WO1990012318A1 - Assays using albumin-tetrazolium interaction - Google Patents

Assays using albumin-tetrazolium interaction Download PDF

Info

Publication number
WO1990012318A1
WO1990012318A1 PCT/GB1990/000552 GB9000552W WO9012318A1 WO 1990012318 A1 WO1990012318 A1 WO 1990012318A1 GB 9000552 W GB9000552 W GB 9000552W WO 9012318 A1 WO9012318 A1 WO 9012318A1
Authority
WO
WIPO (PCT)
Prior art keywords
albumin
reducing agent
tetrazolium
compound
sample
Prior art date
Application number
PCT/GB1990/000552
Other languages
English (en)
French (fr)
Inventor
Anthony Atkinson
Peter Hammond
Roger James Hinton
Julie Miller
Christopher Phillip Price
Original Assignee
Public Health Laboratory Service Board
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Public Health Laboratory Service Board filed Critical Public Health Laboratory Service Board
Publication of WO1990012318A1 publication Critical patent/WO1990012318A1/en
Priority to NO91913965A priority Critical patent/NO913965L/no
Priority to KR1019910701311A priority patent/KR920701821A/ko
Priority to FI914812A priority patent/FI914812A0/fi

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green

Definitions

  • bromocresol purple does not have interspecies albumin specificity; many calibrators contain either bovine or equine albumin standards, which give an absorbance too far below that of human albumin to be of value. Further, heparin is reported to interfere (Perry, B.W. & Douman, B.T., Clin.Chem.
  • the method is therefore most suited to the assay of aqueous samples believed to contain albumin, especially biological samples such as bodily fluids, and in particular blood serum, which may be used directly without any pretreatment, or urine etc.
  • albumin especially biological samples such as bodily fluids, and in particular blood serum, which may be used directly without any pretreatment, or urine etc.
  • the quantity of tetrazolium salt and reducing agent present in the reaction mixture should be in excess of that expected to interact with the albumin in the sample so that the quantity of albumin present is the limiting factor.
  • Fig 1 which shows the hyperchromic shift on reduction of a
  • Fig 8 which shows absorbance versus paracetamol concentration.
  • Fig 9 which shows absorbance versus chloramphenicol

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
PCT/GB1990/000552 1989-04-12 1990-04-11 Assays using albumin-tetrazolium interaction WO1990012318A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
NO91913965A NO913965L (no) 1989-04-12 1991-10-10 Fremgangsmaate for reduksjon av en tetrazoliumforbindelse og anvendelse av fremgangsmaaten ved analyse av albumin og andre reduserende midler
KR1019910701311A KR920701821A (ko) 1989-04-12 1991-10-11 알부민-테트라졸륨 상호작용을 이용한 분석방법
FI914812A FI914812A0 (fi) 1989-04-12 1991-10-11 Analyser med anvaendning av verkan mellan albumin och tetrazolium.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8908182.2 1989-04-12
GB898908182A GB8908182D0 (en) 1989-04-12 1989-04-12 Method for assay of albumin

Publications (1)

Publication Number Publication Date
WO1990012318A1 true WO1990012318A1 (en) 1990-10-18

Family

ID=10654837

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1990/000552 WO1990012318A1 (en) 1989-04-12 1990-04-11 Assays using albumin-tetrazolium interaction

Country Status (9)

Country Link
EP (1) EP0467938A1 (ja)
JP (1) JPH04504467A (ja)
KR (1) KR920701821A (ja)
AU (1) AU5427990A (ja)
CA (1) CA2050884A1 (ja)
FI (1) FI914812A0 (ja)
GB (2) GB8908182D0 (ja)
HU (1) HU903644D0 (ja)
WO (1) WO1990012318A1 (ja)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0476457A2 (en) * 1990-09-19 1992-03-25 Bayer Corporation 2-Benzothiazolyl tetrazolium salt indicators
EP0476454A1 (en) * 1990-09-19 1992-03-25 Bayer Corporation Analytical method using tetrazolium salt indicators having a reflectance plateau
WO1993025905A1 (en) * 1992-06-05 1993-12-23 International Murex Technologies Corporation Immunoassay method
US5300637A (en) * 1990-09-19 1994-04-05 Miles Inc. 2-benzothiazolyl tetrazolium salt indicators
US6225074B1 (en) * 1997-08-18 2001-05-01 Dennis Wright Direct chloramphenicol acetyl transferase assay
WO2012052746A3 (en) * 2010-10-18 2012-06-07 University College Cardiff Consultants Limited Method and device for the detection of sulphur containing species

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017090631A1 (ja) * 2015-11-24 2017-06-01 国立大学法人 東京大学 細胞外代謝物を検出するための蛍光プローブ及び当該蛍光プローブを用いるスクリーニング方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0279988A1 (en) * 1986-12-16 1988-08-31 Enzymatics, Inc. Digital threshold colour control system
WO1988008882A1 (en) * 1987-05-08 1988-11-17 The Public Health Laboratory Service Board Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0279988A1 (en) * 1986-12-16 1988-08-31 Enzymatics, Inc. Digital threshold colour control system
WO1988008882A1 (en) * 1987-05-08 1988-11-17 The Public Health Laboratory Service Board Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Histochemical Journal, Vol. 20, 1988, (Londen, GB), C.J.F. VAN NOORDEN: "On the Role of Oxygen in Dehydrogenase Reactions using Tetrazolium Salts", pages 587-593 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0476457A2 (en) * 1990-09-19 1992-03-25 Bayer Corporation 2-Benzothiazolyl tetrazolium salt indicators
EP0476454A1 (en) * 1990-09-19 1992-03-25 Bayer Corporation Analytical method using tetrazolium salt indicators having a reflectance plateau
EP0476457A3 (en) * 1990-09-19 1992-09-02 Miles Inc. 2-benzothiazolyl tetrazolium salt indicators
US5300637A (en) * 1990-09-19 1994-04-05 Miles Inc. 2-benzothiazolyl tetrazolium salt indicators
WO1993025905A1 (en) * 1992-06-05 1993-12-23 International Murex Technologies Corporation Immunoassay method
US6225074B1 (en) * 1997-08-18 2001-05-01 Dennis Wright Direct chloramphenicol acetyl transferase assay
WO2012052746A3 (en) * 2010-10-18 2012-06-07 University College Cardiff Consultants Limited Method and device for the detection of sulphur containing species
US20130224873A1 (en) * 2010-10-18 2013-08-29 University College Cardiff Consultants Limited Method and device for the detection of sulphur containing species
US9448212B2 (en) * 2010-10-18 2016-09-20 University College Cardiff Consultants Limited Method for the detection of sulphur containing phosphorylating agents

Also Published As

Publication number Publication date
GB2250026A (en) 1992-05-27
FI914812A0 (fi) 1991-10-11
AU5427990A (en) 1990-11-05
EP0467938A1 (en) 1992-01-29
GB9121010D0 (en) 1992-01-15
GB2250026B (en) 1993-02-10
KR920701821A (ko) 1992-08-12
CA2050884A1 (en) 1990-10-13
GB8908182D0 (en) 1989-05-24
HU903644D0 (en) 1992-02-28
JPH04504467A (ja) 1992-08-06

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