WO1990008828A3 - Procedes moleculaires de production de semences hybrides - Google Patents

Procedes moleculaires de production de semences hybrides Download PDF

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Publication number
WO1990008828A3
WO1990008828A3 PCT/CA1990/000037 CA9000037W WO9008828A3 WO 1990008828 A3 WO1990008828 A3 WO 1990008828A3 CA 9000037 W CA9000037 W CA 9000037W WO 9008828 A3 WO9008828 A3 WO 9008828A3
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WO
WIPO (PCT)
Prior art keywords
hybrid seed
plants
male
fertile
sterile
Prior art date
Application number
PCT/CA1990/000037
Other languages
English (en)
Other versions
WO1990008828A2 (fr
Inventor
Steven F Fabijanski
Diego Albani
Laurian Sylvio Robert
Paul G Arnison
Original Assignee
Paladin Hybrids Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27232625&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1990008828(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from NZ227835A external-priority patent/NZ227835A/xx
Application filed by Paladin Hybrids Inc filed Critical Paladin Hybrids Inc
Priority to AU50372/90A priority Critical patent/AU655574C/en
Priority to AT90902754T priority patent/ATE294872T1/de
Priority to EP90902754A priority patent/EP0456706B1/fr
Priority to DE69034190T priority patent/DE69034190T2/de
Priority to CA002158333A priority patent/CA2158333A1/fr
Publication of WO1990008828A2 publication Critical patent/WO1990008828A2/fr
Publication of WO1990008828A3 publication Critical patent/WO1990008828A3/fr
Priority to US08/484,838 priority patent/US6737560B1/en
Priority to US08/477,265 priority patent/US6198023B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01031—Beta-glucuronidase (3.2.1.31)

Abstract

Le procédé décrit, qui sert à produire des semences hybrides fertiles ou des semences hybrides composées de semences fertiles et de semences stériles, utilise des plantes stériles mâles créées par des techniques moléculaires permettant de manipuler un gène antisensoriel et d'autres gènes qui sont capables de réguler la production de pollen fertile dans les plantes. Des techniques de transformation sont utilisées pour introduire dans les plantes des structures contenant des ADN antisensoriels et d'autres gènes. Ces plantes sont fonctionnellement stériles mâles et sont utiles pour la production de semences hybrides par croisement de ces plantes stériles mâles avec du pollen provenant de plantes fertiles mâles. Ainsi, la production de semences hybrides est simplifiée et améliorée grâce à la présente invention et son application peut être étendue à des espèces de cultures végétales pour lesquelles des procédés de production de semences hybrides commercialement acceptables ne sont pas couramment disponibles.
PCT/CA1990/000037 1988-02-03 1990-02-02 Procedes moleculaires de production de semences hybrides WO1990008828A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU50372/90A AU655574C (en) 1988-02-03 1990-02-02 Molecular methods of hybrid seed production
AT90902754T ATE294872T1 (de) 1989-02-02 1990-02-02 Molekulare verfahren zur vermehrung von hybriden saaten
EP90902754A EP0456706B1 (fr) 1989-02-02 1990-02-02 Procedes moleculaires de production de semences hybrides
DE69034190T DE69034190T2 (de) 1989-02-02 1990-02-02 Molekulare verfahren zur vermehrung von hybriden saaten
CA002158333A CA2158333A1 (fr) 1990-02-02 1990-07-20 Methodes moleculaires pour la production de semences hybrides
US08/484,838 US6737560B1 (en) 1988-02-03 1995-06-07 Molecular methods of hybrid seed production
US08/477,265 US6198023B1 (en) 1988-02-03 1995-06-07 Molecular methods of hybrid seed production

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
NZ227835 1989-02-02
NZ227835A NZ227835A (en) 1988-02-03 1989-02-02 Antisense gene systems of pollination control for hybrid seed production
US30643889A 1989-02-03 1989-02-03
US306,438 1989-02-03
EP89301053.8 1989-02-03
EP89301053A EP0329308B1 (fr) 1988-02-03 1989-02-03 Systèmes de contrôle de la pollinisation utilisant des gènes antisens pour la production de semences hybrides

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US30643889A Continuation-In-Part 1988-02-03 1989-02-03

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US55691790A Continuation-In-Part 1988-02-03 1990-07-20
US27651094A Continuation 1988-02-03 1994-07-14

Publications (2)

Publication Number Publication Date
WO1990008828A2 WO1990008828A2 (fr) 1990-08-09
WO1990008828A3 true WO1990008828A3 (fr) 1990-10-18

Family

ID=27232625

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1990/000037 WO1990008828A2 (fr) 1988-02-03 1990-02-02 Procedes moleculaires de production de semences hybrides

Country Status (6)

Country Link
EP (1) EP0456706B1 (fr)
JP (2) JPH04504355A (fr)
AT (1) ATE294872T1 (fr)
AU (1) AU1628695A (fr)
DE (1) DE69034190T2 (fr)
WO (1) WO1990008828A2 (fr)

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CHEMICAL ABSTRACTS, Volume 106, 1987, (Columbus, Ohio, US), J.R. STINSON et al.: "Genes Expressed in the Male Gametophyte of Flowering Plants and Their Isolation", see page 175, Abstract 150569p, & Plant Physiol. 1987, 83(2), 442-7 *
Journal of Cellular Biochemistry, Supplement 12c, 1988, UCLA Symposia on Molecular & Cellular Biology, The Molecular Basis of Plant Development, 26 March - 2 April 1988, Alan R. Liss, Inc., (New York, US), C.S. GASSER et al.: "Analysis of Floral Specific Genes", page 137, Abstract L 021 *
Journal of Cellular Biochemistry, Supplement 12C, 1988, UCLA Symposia on Molecular & Cellular Biology, The Molecular Basis of Plant Development, 26 March - 2 April 1988, Alan R. Liss, Inc., (New York, US), J.I. MEDFORD et al.: "Manipulation of Auxin, Cytokinin and GUS Levels using the Maize HSP70 Promoter", page 212, Abstract L 616 *
Proc. Natl. Acad. Sci. USA, Volume 84, December 1987, S.J. ROTHSTEIN et al.: "Stable and Heritable Inhibition of the Expression of Nopaline Synthase in Tobacco Expressing Antisense RNA", pages 8439-8443 *
Proc. Natl. Acad. Sci. USA, Volume 85, June 1988, A.J. DELAUNEY et al.: "A Stable Bifunctional Antisense Transcript Inhibiting Gene Expression in Transgenic Plants", pages 4300-4304 *
The Plant Cell, Volume 1, January 1989, American Society of Plant Physiologists, C.S. GASSER et al.: "Isolation of Tissue-Specific cDNAs From Tomato Pistils", pages 15-24 *
UCLA Symp. Mol. Cell. Biol., New Ser., Volume 92, The Molecular Basis of Plant Development, Proceedings of an E.I. du Pont de Nemours-UCLA Symposium, Steamboat Springs, Colorado, 26 March - 2 April 1988, Alan R. Liss, Inc., (New York, US), J.P. MASCARENHAS: "Characterization of Genes that Are Expressed in Pollen", pages 99-105 *

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AU5037290A (en) 1990-08-24
EP0456706B1 (fr) 2005-05-04
DE69034190D1 (de) 2005-06-09
JPH09182589A (ja) 1997-07-15
AU655574B2 (en) 1995-01-05
ATE294872T1 (de) 2005-05-15
AU1628695A (en) 1995-08-03
EP0456706A1 (fr) 1991-11-21
JPH04504355A (ja) 1992-08-06
DE69034190T2 (de) 2006-02-23
WO1990008828A2 (fr) 1990-08-09

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