WO1989010761A1 - Procede et dispositif pour inactiver un virus dans un milieu porteur - Google Patents

Procede et dispositif pour inactiver un virus dans un milieu porteur Download PDF

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Publication number
WO1989010761A1
WO1989010761A1 PCT/AT1989/000033 AT8900033W WO8910761A1 WO 1989010761 A1 WO1989010761 A1 WO 1989010761A1 AT 8900033 W AT8900033 W AT 8900033W WO 8910761 A1 WO8910761 A1 WO 8910761A1
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WO
WIPO (PCT)
Prior art keywords
viruses
carrier medium
sec
membrane
sound waves
Prior art date
Application number
PCT/AT1989/000033
Other languages
German (de)
English (en)
Inventor
Bertwin Langenecker
Original Assignee
Bertwin Langenecker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bertwin Langenecker filed Critical Bertwin Langenecker
Publication of WO1989010761A1 publication Critical patent/WO1989010761A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
    • A61K41/10—Inactivation or decontamination of a medicinal preparation prior to administration to an animal or a person
    • A61K41/13—Inactivation or decontamination of a medicinal preparation prior to administration to an animal or a person by ultrasonic waves
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits

Definitions

  • the invention relates to a method for inactivating viruses contained in a carrier medium, in particular blood, and a device for carrying out the method.
  • the invention relates to a method and a device with which the blood of living people or animals with infectious diseases such as AIDS (acquired immuno deficiency syndrome) as a result of infection by LAV / HTLV III (Lymphadenopathy-Associated Virus / Human-T- Lymphotropic Virus), recently also called HIV (Human Immunodeficiency Virus), or, for example, infectious horse anemia due to the morphologically and genetically related EIAV (Equine Infectious Anemia Virus), which is also a lentivirus, for inactivating the viruses contained therein can be treated extracorporeally.
  • infectious diseases such as AIDS (acquired immuno deficiency syndrome) as a result of infection by LAV / HTLV III (Lymphadenopathy-Associated Virus / Human-T- Lymphotropic Virus), recently also
  • the process according to the invention is essentially characterized in that the carrier medium is exposed to high-frequency sound waves together with the viruses. It has been shown in laboratory tests that the viruses are broken up in a high-frequency sound field concentrated to a high energy density and thereby inactivated, so that lasting success can be achieved in a surprising manner.
  • the method according to the invention can be used not only for inactivating viruses contained in human blood, but also for viruses contained in any liquid carrier medium, for example thus also for carrying out experiments in which the carrier medium does not run out
  • Activation of processes such as fermentation processes or the like. apply and thereby shorten the activation time and / or increase the yield of the extraction of, for example, anabolic, breathing-demanding, low-molecular materials for physical, therapeutic and cell and tissue culture purposes (CH-PS 651063).
  • a vibration generator is located in a medium, for example in a carrier medium, the vibrations are transmitted to the medium. With each oscillation movement the medium is pushed and this impacting process propagates in the form of successive compression and thinning points. This effect is linked to the presence of matter.
  • the present invention is based on the established effect that only occurs when high-frequency sound waves are applied to a carrier medium, since the viruses in the carrier medium are torn apart as a result of the dilation and compression.
  • Hz can be used.
  • the use of this frequency range means that the wavelength of such hypersonic waves etv / a is of the same order of magnitude as that of the viruses to be inactivated, namely around 100 nm, so that resonance effects can occur in which the amplitudes significantly exceed the tensile strength of the viruses.
  • Such an acoustic pressure amplitude is matched to the strength properties of the virus, thus ensuring that the virus is completely broken open.
  • the viruses inactivated by treatment with high-frequency sound waves can, according to the invention, be removed from the carrier medium by dialysis.
  • Extracorporeal dialysis treatment of the blood is known in human medicine for kidney diseases (artificial kidney).
  • the inactivated viruses on semipermeable membranes are physically removed from the carrier medium by dialysis.
  • the device according to the invention is essentially characterized in that an ultrasound transmitter is arranged in a housing which is provided with a feed opening and a discharge opening for the carrier medium containing the viruses, with whose high-frequency sound field the viruses contained in the carrier medium are broken up.
  • a membrane which preferably consists of plastic or sintered material or cermet. Cermet is a material compressed from ceramic substances and metals.
  • the materials indicated, from which the membrane is preferably made, have a relatively homogeneous pore size, that is to say all the pores have essentially the same size, and moreover the preferred average pore size given below without additional measures.
  • This membrane preferably has an average pore size between 150 and 200 microns, so that it is ensured that both lentiviruses, the dimensions of which are 100 to 150 nm, as well as the larger phagocytes, which are in the blood, can enter the interior of the housing through the membrane .
  • both lentiviruses the dimensions of which are 100 to 150 nm, as well as the larger phagocytes, which are in the blood, can enter the interior of the housing through the membrane .
  • it is according to a further feature of the device according to the invention advantageous if at least one reflector is provided in the housing, which focuses the sound waves emitted by the ultrasound transmitter onto the membrane, so that a effective sonication of the carrier medium containing the viruses takes place immediately upon entry into the housing through the membrane.
  • the discharge opening is connected to the input of a dialyzer, in which the inactivated viruses are separated from the transport medium and which is provided with an outlet for the removal of the dialysate.
  • dialyzers are already known per se for blood purification in kidney diseases and are referred to in this context as "artificial kidney”.
  • the dialyzer can be provided with a device for admixing chemical treatment agents, for example chemotherapeutic agents, to the means of transport cleaned of the inactivated viruses, in particular those substances which contribute to selectively damaging the viruses to be inactivated, especially those which are caused by the treatment with high-frequency sound waves was not completely detected and permanently inactivated, so that it is easier for the phagocytes present in the blood to digest the debris of the inactivated viruses which had not been removed from the transport medium in the dialyzer.
  • chemical treatment agents for example chemotherapeutic agents
  • the device according to the invention is compact if the ultrasound transmitter and the dialyzer are arranged in a common housing.
  • a device according to the invention is schematically illustrated in the drawing using an exemplary embodiment, the method according to the invention also being described in more detail in connection with the drawing.
  • 1 shows the overall device required for the treatment of blood containing viruses.
  • 2 shows a cross section through the housing containing the ultrasound transmitter and the dialyzer.
  • the carrier medium containing the viruses to be inactivated for example ce ⁇ blood of a living patient, is connected via a supply line 2 which is connected to the patient's artery via an erceriovenous shunt. sen, the feed opening 2 '.
  • ⁇ housing 1 supplied by means of a pump 3.
  • the arterial pressure is monitored by a vacuum monitor 4. 5 denotes a device via which chemical
  • Substances e.g. Heparin, to prevent blood from clotting, be added to it during treatment.
  • the treatment of the blood containing the viruses by the ultrasound generator is described in more detail in connection with FIG. 2.
  • the blood passes through a discharge opening 2 "to a dialyzer 6 of a type known per se, in which the inactivated viruses are separated from the blood on a semi-permeable membrane 7.
  • the dialysate is transported away via exits 8.
  • the dialysate subjected to a customary medical treatment which is not essential in connection with the present invention and is therefore not described in more detail.
  • the dialyzer 6 is connected to a device 9 for adding chemical treatment agents to the dialysate.
  • this device 9 can be used, for example, to add substances which make it easier for the phagocytes to digest the droplets of the inactivated viruses which were not removed from the blood in the dialyzer.
  • the blood, from which the inactivated viruses were secreted in the dialyzer 6, is supplied to the patient's vein via at least one line 12.
  • a drop chamber 10 is arranged in the line 12 and communicates with an air detector 11.
  • the venous pressure is monitored by a pressure monitor 13 and adjusted by suitable means.
  • the treatment of the blood containing the viruses in the housing 1 by an ultrasound transmitter 15 will now be explained with reference to FIG.
  • the feed opening 2 ′ is covered by a membrane 14, through which the blood containing viruses enters the interior of the housing 1.
  • the hypersonic field emanating from the ultrasound transmitter 15, which consists of a piezo-ceramic or other type of converter, is focused on the membrane 14 with the aid of reflectors 16 and 17, so that the blood supplied via the membrane is exposed to sound waves of high energy density.
  • the membrane 14 is preferably made of plastic or sintered material or cermet and has an average width of 150 to 200 microns, so that both the viruses and the larger phagocytes contained in the blood would enter the interior of the housing 1.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • External Artificial Organs (AREA)

Abstract

Pour inactiver un virus dans un milieu porteur, notamment dans le sang, le virus et son milieu sont soumis à un champ d'ultrasons à haute densité au moyen d'un générateur d'ultrasons (15). Les ondes ultrasonores émises par le générateur (15) sont focalisées par des réflecteurs (16, 17) sur une membrane (14) par l'intermédiaire de laquelle le sang contenant les virus pénètre dans le logement (1) renfermant le générateur d'ultrasons (15). Les virus inactivés de cette façon sont séparés du sang dans un dialyseur (6).
PCT/AT1989/000033 1988-05-03 1989-04-11 Procede et dispositif pour inactiver un virus dans un milieu porteur WO1989010761A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0113488A AT389889B (de) 1988-05-03 1988-05-03 Verfahren und vorrichtung zum inaktivieren von in einem traegermedium enthaltenen viren
ATA1134/88 1988-05-03

Publications (1)

Publication Number Publication Date
WO1989010761A1 true WO1989010761A1 (fr) 1989-11-16

Family

ID=3507336

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AT1989/000033 WO1989010761A1 (fr) 1988-05-03 1989-04-11 Procede et dispositif pour inactiver un virus dans un milieu porteur

Country Status (2)

Country Link
AT (1) AT389889B (fr)
WO (1) WO1989010761A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2285220A (en) * 1993-12-28 1995-07-05 Gabriele Tombesi Ultrasonic anti-allergen apparatus
WO2023199101A1 (fr) * 2022-04-16 2023-10-19 Garcia Rojas, Christian Javier Dispositif portable émetteur d'ondes ultrasonores
WO2023235005A1 (fr) * 2022-05-31 2023-12-07 Boundless Science Llc Procédé et appareil pour un transport amélioré

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT406778B (de) * 1996-04-09 2000-09-25 Immuno Ag Verfahren zur desintegration von nucleinsäuren und herstellung von qualitätsgesicherten biologischen produkten
US7306737B2 (en) 2002-04-10 2007-12-11 Bertwin Langenecker Inactivation of microorganisms and virus in liquids and sludge
US7770830B1 (en) 2007-04-26 2010-08-10 Bertwin Langenecker Method and apparatus for desalination of seawater
DE202012104788U1 (de) 2012-12-10 2013-01-04 HSD Industriebeläge GmbH Schalungselement für den Fußbodenbau

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE949253C (de) * 1954-11-20 1956-09-13 Schoeller & Co Elektrotech Verfahren zum Sterilisieren von Fluessigkeiten durch Behandeln mit Ultraschall
EP0261032A1 (fr) * 1986-09-16 1988-03-23 Association Regionale De Transfusion Sanguine Procédé d'inactivation par ozonolyse de micro-organismes contaminants présents dans des matériaux biologiques essentiellement protéiques, les produits obtenus et leurs applications biologiques et analytiques

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH651063A5 (de) * 1981-05-14 1985-08-30 Elkawi Ag Verfahren zur gewinnung anaboler, atmungsfoerdernder, niedermolekularer wirkstoffe fuer prophylaktische, therapeutische, zell- und gewebekulturtechnische zwecke.
US4727027A (en) * 1983-05-02 1988-02-23 Diamond Scientific Co. Photochemical decontamination treatment of whole blood or blood components
JPS6251990A (ja) * 1985-08-29 1987-03-06 Nippon Zeon Co Ltd 微生物菌体の処理方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE949253C (de) * 1954-11-20 1956-09-13 Schoeller & Co Elektrotech Verfahren zum Sterilisieren von Fluessigkeiten durch Behandeln mit Ultraschall
EP0261032A1 (fr) * 1986-09-16 1988-03-23 Association Regionale De Transfusion Sanguine Procédé d'inactivation par ozonolyse de micro-organismes contaminants présents dans des matériaux biologiques essentiellement protéiques, les produits obtenus et leurs applications biologiques et analytiques

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2285220A (en) * 1993-12-28 1995-07-05 Gabriele Tombesi Ultrasonic anti-allergen apparatus
WO2023199101A1 (fr) * 2022-04-16 2023-10-19 Garcia Rojas, Christian Javier Dispositif portable émetteur d'ondes ultrasonores
WO2023235005A1 (fr) * 2022-05-31 2023-12-07 Boundless Science Llc Procédé et appareil pour un transport amélioré

Also Published As

Publication number Publication date
ATA113488A (de) 1989-07-15
AT389889B (de) 1990-02-12

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