WO1989009824A3 - Synthese de genes - Google Patents

Synthese de genes Download PDF

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Publication number
WO1989009824A3
WO1989009824A3 PCT/GB1989/000384 GB8900384W WO8909824A3 WO 1989009824 A3 WO1989009824 A3 WO 1989009824A3 GB 8900384 W GB8900384 W GB 8900384W WO 8909824 A3 WO8909824 A3 WO 8909824A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
double stranded
prepared
restriction sites
hybrid
Prior art date
Application number
PCT/GB1989/000384
Other languages
English (en)
Other versions
WO1989009824A2 (fr
Inventor
Richard Mark Edwards
Sally Elizabeth Adams
Original Assignee
British Bio Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by British Bio Technology filed Critical British Bio Technology
Publication of WO1989009824A2 publication Critical patent/WO1989009824A2/fr
Priority to BR909004512A priority Critical patent/BR9004512A/pt
Publication of WO1989009824A3 publication Critical patent/WO1989009824A3/fr
Priority to DK247890A priority patent/DK247890D0/da
Priority to NO904434A priority patent/NO904434D0/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

On peut préparer de l'ADN à double brin (ADN-ds) en préparant de l'ADN hybride contenant une partie monobrin et une partie à double brin, et en procédant à la réparation de l'écart in vivo dans l'ADN hybride. On prépare l'ADN hybride par synthèse d'un monobrin d'ADN puis par introduction dudit monobrin dans l'ADN à double brin. On peut utiliser ce procédé de synthèse pour fabriquer des gènes synthétiques, comprenant de l'ADN synthétique codant pour la protéine TAT du VIH-1, lequel ADN possède la séquence (I) et comporte des sites de restriction utiles. Sont également compris des sites de restriction de flanc permettant de simplifier l'incorporation du gène dans nimporte quel système d'expression voulu.
PCT/GB1989/000384 1988-04-15 1989-04-14 Synthese de genes WO1989009824A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BR909004512A BR9004512A (pt) 1989-04-14 1990-01-09 Processo para a preparacao de um acido ditiofosforico
DK247890A DK247890D0 (da) 1988-04-15 1990-10-12 Gensyntese
NO904434A NO904434D0 (no) 1988-04-15 1990-10-12 Gensyntese.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8808892.7 1988-04-15
GB888808892A GB8808892D0 (en) 1988-04-15 1988-04-15 Gene synthesis

Publications (2)

Publication Number Publication Date
WO1989009824A2 WO1989009824A2 (fr) 1989-10-19
WO1989009824A3 true WO1989009824A3 (fr) 1990-01-11

Family

ID=10635228

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1989/000384 WO1989009824A2 (fr) 1988-04-15 1989-04-14 Synthese de genes

Country Status (7)

Country Link
US (1) US5158877A (fr)
EP (2) EP0593132A1 (fr)
JP (1) JPH03504797A (fr)
AU (2) AU623404B2 (fr)
DK (1) DK247890D0 (fr)
GB (1) GB8808892D0 (fr)
WO (1) WO1989009824A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU649066B2 (en) * 1990-07-25 1994-05-12 Syngene, Inc. Circular extension for generating multiple nucleic acid complements
US6110668A (en) * 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
US5891994A (en) * 1997-07-11 1999-04-06 Thymon L.L.C. Methods and compositions for impairing multiplication of HIV-1
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
US6399067B1 (en) 2000-04-28 2002-06-04 Thymon L.L.C. Methods and compositions for impairing multiplication of HIV-1
EP1851247A2 (fr) 2005-02-15 2007-11-07 Thymon L.L.C. Methodes et compositions permettant d'alterer la multiplication de vih-1
EP1762627A1 (fr) 2005-09-09 2007-03-14 Qiagen GmbH Procédé pour l'activation d'acides nucléiques pour effectuer une réaction d'une polymérase
EP3102676A1 (fr) * 2013-12-09 2016-12-14 Integrated DNA Technologies Inc. Séquences d'acides nucléiques longs contenant des régions variables

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0253193A2 (fr) * 1986-07-08 1988-01-20 Abbott Laboratories Méthode de mutagénèse par réparation dirigée à l'aide d'oligonucléotides d'une rupture de brin

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE49998T1 (de) * 1983-05-11 1990-02-15 Labofina Sa Klonierungsvektoren, die eine restriktionsstellenbank enthalten und deren herstellung.
GB8525321D0 (en) * 1985-10-15 1985-11-20 Glaxo Group Ltd Chemical compounds
IL81568A0 (en) * 1986-02-14 1987-09-16 Us Commerce Plasmids which inhibit human t-cell lymphotropic virus type iii replication
IE66597B1 (en) * 1989-05-10 1996-01-24 Akzo Nv Method for the synthesis of ribonucleic acid (RNA)

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0253193A2 (fr) * 1986-07-08 1988-01-20 Abbott Laboratories Méthode de mutagénèse par réparation dirigée à l'aide d'oligonucléotides d'une rupture de brin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification

Also Published As

Publication number Publication date
EP0593132A1 (fr) 1994-04-20
DK247890A (da) 1990-10-12
DK247890D0 (da) 1990-10-12
AU1052792A (en) 1992-04-16
JPH03504797A (ja) 1991-10-24
GB8808892D0 (en) 1988-05-18
AU3449589A (en) 1989-11-03
EP0400093A1 (fr) 1990-12-05
AU623404B2 (en) 1992-05-14
US5158877A (en) 1992-10-27
AU634926B2 (en) 1993-03-04
WO1989009824A2 (fr) 1989-10-19

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