Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
  • G01N30/02—Column chromatography
  • G01N30/26—Conditioning of the fluid carrier; Flow patterns
  • G01N30/28—Control of physical parameters of the fluid carrier
  • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
  • G01N30/02—Column chromatography
  • G01N30/62—Detectors specially adapted therefor
  • G01N2030/621—Detectors specially adapted therefor signal-to-noise ratio
  • G01N2030/625—Detectors specially adapted therefor signal-to-noise ratio by measuring reference material, e.g. carrier without sample

Definitions

• the mobile phase carries the rest of the previous - 2 - ⁇ b traces ⁇ as ⁇ v ⁇ enn ⁇ g ⁇ s ⁇ ben ⁇ a and d ⁇ ugie ⁇ imesi, ⁇ - ⁇ edelyayuschie for chuvs ⁇ vi ⁇ elnB ⁇ de ⁇ e ⁇ v ⁇ a ⁇ a ⁇ e ⁇ measured neny and ⁇ n ⁇ v ⁇ g ⁇ signal u ⁇ ven, ⁇ y m ⁇ zhe ⁇ by ⁇ ⁇ e- ⁇ emennym and znachi ⁇ eln ⁇ ⁇ licha ⁇ sya ⁇ sv ⁇ emu u ⁇ vnyu ⁇ 5 ⁇ n ⁇ v ⁇ g ⁇ signal s ⁇ avni ⁇ eln ⁇ y ⁇ yuve ⁇ y. Otherwise, a well-known method of processing is performed due to the high rate of analysis due to the increased quantity of the eluent, which is not necessary for analyzing the presence of pressure.
• the volume of the eluent needed for one analysis is 1-2 times the volume of the diluent tank. Finally, there is little effect on the composition of the liquid in the diluent capacity of the eluent coming out of the column, nevertheless
• the eluent 30 notably distinguished from the eluent, who did not simply let go through the column.
• the eluent may contain substances that are lost as a result of the dissolution of the benthic or of waste from impurities that are washed away from the previous analysis. Otherwise, the eluent itself can change its
• the high sensitivity signals of the measured signal line may be at different levels of the registers of the registers. If the compensation of changes in the system and properties of the eluent is not sufficient,
• 35 of the analyzes compares and for the results of the comparisons is based on the composition of the analyzed material, according to the invention, as a result of which is commercially available - 5 - ent, which immediately downloads through a quick-to-use microcircuit without introducing it into the analyzer that is being analyzed
• ⁇ ⁇ a- 30 ches ⁇ ve ⁇ n ⁇ ln ⁇ g ⁇ ⁇ as ⁇ v ⁇ a is ⁇ lzuyu ⁇ elyuen ⁇ , ⁇ y ⁇ anivayu ⁇ che ⁇ ez.
• Proteins were separated from a mixture of myoglobin, egg albumin, serum albumin and feridine. - 9 - The eluent was acetic acid with added water of 5% by volume ”
• Example II For separation, we used a 3-dimensional microchip, the same size as in Example I, which was stuffed with a large stream of 5 pieces.
• the average size of grain is 5–6 ⁇ m ⁇
• the minimum available quantity of material is 2 ng 25 4.
• the lower range of equipment is 0.5 ng.

Landscapes

• Physics & Mathematics (AREA)
• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Analytical Chemistry (AREA)
• Biochemistry (AREA)
• General Health & Medical Sciences (AREA)
• General Physics & Mathematics (AREA)
• Immunology (AREA)
• Pathology (AREA)
• Treatment Of Liquids With Adsorbents In General (AREA)
• Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Priority Applications (7)

Applications Claiming Priority (1)

Publications (1)

Family

ID=21617116

Family Applications (1)

Country Status (7)

Citations (3)

• 1987
  • 1987-06-16 JP JP62505405A patent/JPH01503805A/ja active Pending
  • 1987-06-16 DE DE873790973T patent/DE3790973T1/de not_active Withdrawn
  • 1987-06-16 FI FI890677A patent/FI890677A0/fi not_active IP Right Cessation
  • 1987-06-16 WO PCT/SU1987/000069 patent/WO1988010426A1/ru not_active Ceased
  • 1987-06-16 CH CH582/89A patent/CH675304A5/de not_active IP Right Cessation
• 1989
  • 1989-02-10 GB GB8902975A patent/GB2217449B/en not_active Expired - Fee Related
  • 1989-02-10 SE SE8900468A patent/SE462664B/sv not_active Application Discontinuation

Patent Citations (3)

Non-Patent Citations (1)

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