WO1988005993A3 - Aminopeptidase servant a extraire de la methionine a terminaison n a partir de proteines, et proteines preparees a partir de cette aminopeptidase - Google Patents

Aminopeptidase servant a extraire de la methionine a terminaison n a partir de proteines, et proteines preparees a partir de cette aminopeptidase Download PDF

Info

Publication number
WO1988005993A3
WO1988005993A3 PCT/EP1988/000096 EP8800096W WO8805993A3 WO 1988005993 A3 WO1988005993 A3 WO 1988005993A3 EP 8800096 W EP8800096 W EP 8800096W WO 8805993 A3 WO8805993 A3 WO 8805993A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
aminopeptidase
peptidase
strains
terminal methionine
Prior art date
Application number
PCT/EP1988/000096
Other languages
English (en)
Other versions
WO1988005993A2 (fr
Inventor
Charles G Miller
Rao Movva
Paul Wingfield
Gonzola J Mazzei
Original Assignee
Biogen Nv
Charles G Miller
Rao Movva
Paul Wingfield
Gonzola J Mazzei
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Nv, Charles G Miller, Rao Movva, Paul Wingfield, Gonzola J Mazzei filed Critical Biogen Nv
Priority to JP63504077A priority Critical patent/JPH03502400A/ja
Publication of WO1988005993A2 publication Critical patent/WO1988005993A2/fr
Publication of WO1988005993A3 publication Critical patent/WO1988005993A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Abstract

Une aminopeptidase, appelée ici peptidase M qui extrait uniquement de la méthionine à terminaison N, des souches de microorganismes qui produisent cette aminopeptidase, et des polypeptides traités avec cette aminopeptidase sont décrits. La peptidase M peut être utilisée pour le clivage de protéines de polypeptides in vitro, ou l'ADN des souches qui produisent la peptidase M peut être utilisé pour transformer un organisme hôte recombinant de façon à préparer des protéines matures in vivo. Les souches qui produisent la peptidase M peuvent elles-mêmes être utilisées comme organismes hôtes recombinants. Des procédés servant à isoler la peptidase M et des procédés permettant d'isoler des souches qui produisent en excès de la peptidase M sont également décrits.
PCT/EP1988/000096 1987-02-10 1988-02-09 Aminopeptidase servant a extraire de la methionine a terminaison n a partir de proteines, et proteines preparees a partir de cette aminopeptidase WO1988005993A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP63504077A JPH03502400A (ja) 1988-02-09 1988-02-09 蛋白質からｎ‐末端メチオニンを除去するアミノペプチダーゼおよびこれにより調製される蛋白質

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB878703015A GB8703015D0 (en) 1987-02-10 1987-02-10 Aminopeptidase
GB8703015 1987-02-10

Publications (2)

Publication Number Publication Date
WO1988005993A2 WO1988005993A2 (fr) 1988-08-25
WO1988005993A3 true WO1988005993A3 (fr) 1988-11-17

Family

ID=10612035

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1988/000096 WO1988005993A2 (fr) 1987-02-10 1988-02-09 Aminopeptidase servant a extraire de la methionine a terminaison n a partir de proteines, et proteines preparees a partir de cette aminopeptidase

Country Status (4)

Country Link
EP (1) EP0366662A1 (fr)
AU (1) AU1719988A (fr)
GB (1) GB8703015D0 (fr)
WO (1) WO1988005993A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5013662A (en) * 1985-09-20 1991-05-07 Cetus Corporation Bacterial methionine n-terminal peptidase
AU4057889A (en) * 1988-08-26 1990-03-23 General Hospital Corporation, The Isolation, purification, and characterization of the methionine-specific aminopeptidases: mas i and mas x
KR900702014A (ko) * 1988-09-13 1990-12-05 원본미기재 아미노펩티다아제 ap1 및 ap122의 단리, 정제 및 특성확인
AU4188189A (en) * 1988-09-13 1990-04-02 General Hospital Corporation, The Isolation, purification, and characterization of the aminopeptidases: mas ii and mas iii
WO1990002814A1 (fr) * 1988-09-13 1990-03-22 The General Hospital Corporation ISOLATION, PURIFICATION ET CARACTERISATION DES AMINOPEPTIDASES AP2, API et APX
KR970010135B1 (ko) * 1993-06-17 1997-06-21 주식회사 엘지화학 신규 아미노펩티다제

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986001229A1 (fr) * 1984-08-16 1986-02-27 Bio-Technology General Corp. Procede d'elimination des acides amines residuels a terminal n d'analogues polypeptides eucaryotes et polypeptides obtenus par ce procede
EP0204527A1 (fr) * 1985-06-04 1986-12-10 Takeda Chemical Industries, Ltd. Elimination de la méthionine N-terminal de protéines méthionyliques
EP0219237A1 (fr) * 1985-09-20 1987-04-22 Cetus Corporation Peptidase bactérienne à N-terminal méthionine, sa préparation et son application

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986001229A1 (fr) * 1984-08-16 1986-02-27 Bio-Technology General Corp. Procede d'elimination des acides amines residuels a terminal n d'analogues polypeptides eucaryotes et polypeptides obtenus par ce procede
EP0204527A1 (fr) * 1985-06-04 1986-12-10 Takeda Chemical Industries, Ltd. Elimination de la méthionine N-terminal de protéines méthionyliques
EP0219237A1 (fr) * 1985-09-20 1987-04-22 Cetus Corporation Peptidase bactérienne à N-terminal méthionine, sa préparation et son application

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Biological Abstracts, RRM/34117064, (Philadelphia, US), A. Ben-Bassat et al.: "N-terminal processing of proteins by methionine aminopeptidase in Escherichia coli" & Abstracts of the Annual Meeting of the American Society for microbiology, vol. 86, 1986 (US), see page 208, abstract no. K-89 *
Biotechnology, vol. 5, no. 8, August 1987, (New York, New York, US), S. Nakagawa et al.: "Enzymatic cleavage of amino terminal methionine from recombinant human interleukin 2 and growth hormone by aminopeptidase M", pages 824-827 *
Journal of Bacteriology, vol. 169, no. 2, February 1987, American Society for Microbiology (US), A. Ben-Bassat et al.: "processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure", pages 751-757 *
Proc. Natl. Acad Sci. USA, vol. 84, May 1987 (Washington, US), C.G. Miller et al.: "N-terminal methionine-specific peptidase in Salmonella typhimurium", pages 2718-2722 *

Also Published As

Publication number Publication date
GB8703015D0 (en) 1987-03-18
EP0366662A1 (fr) 1990-05-09
AU1719988A (en) 1988-09-14
WO1988005993A2 (fr) 1988-08-25

Similar Documents

Publication Publication Date Title
AU587989B2 (en) DMA fragments, expression vectors, proteins, hosts, and process for production of the proteins
AU661696B2 (en) DNA sequences to target proteins to the mammary gland for efficient secretion
EP0347435A4 (en) Dna sequences, recombinant dna molecules and processes for producing soluble t4 proteins
BR8301918A (pt) Proteina,sequencia de dna,veiculo de expressao replicavel,microorganismo ou cultura celular,plasmideo,composicao,cultura de celulas,utilizacao de tal proteina,processo de expressao,processo para producao de um veiculo de expressao e processo para preparacao de uma proteina uroquinase
IE882314L (en) Kluyveromyces as a host strain
ES8500324A1 (es) Procedimiento para la obtencion de proteina heterologa.
DE3884356D1 (de) Verfahren zur Herstellung von Fremdprotein in Hefe, Rekombinant DNS, Transformant.
AU1463097A (en) Helicobacter pylori bacterioferritin
AU4128589A (en) Compression device for the anastomosis of hollow organs
AU3802595A (en) Tripeptidyl aminopeptidase
HUT43106A (en) Dns sequences koding proteins of flesh-i type inhibitor activity and process for producing these proteins
WO1988005993A3 (fr) Aminopeptidase servant a extraire de la methionine a terminaison n a partir de proteines, et proteines preparees a partir de cette aminopeptidase
AU2157888A (en) Novel hiv proteins and peptides useful in the diagnosis, prophylaxis or therapy of aids
PT97383A (pt) Processo para a producao de uma cadeia de globina, de um seu fragmento de ligacao e de hemoglobina, e de composicoes de hemoglobina
WO1995015391A3 (fr) SYSTEME D'EXPRESSION A L'$i(ASPERGILLUS)
AU7440087A (en) Process for isolating and purifying p. falciparum cs protein vaccine expressed in recombinant e. coli
EP0297834A3 (fr) Inhibiteur de l'alpha-amylase
AUPM772494A0 (en) Improvements in production of proteins in host cells
AU617330B2 (en) Human papillomavirus type 41, its DNA and the proteins encoded therefrom
EP0422217A4 (en) Human recombinant placental ribonuclease inhibitor and method of production
FI891020A (fi) Menetelmä proteaasi-inhibiittoriaktiivisuutta omaavien egliinimutanttien valmistamiseksi
ES8608319A1 (es) Procedimiento para la produccion de un integrador alimenti- cio proteicovitaminico a partir de alga espirulina
WO1998023762A3 (fr) Procedes de production d'une proteine de recombinaison
DE3583107D1 (de) Plasmide zur erhoehten produktion von penicillin g-amidase.
CA2159083A1 (fr) Enzyme recombinante de type .alpha.-n-acetylgalactosaminidase et adn-c codant pour cet enzyme

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU DK FI HU JP NO US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE FR GB IT LU NL SE

AK Designated states

Kind code of ref document: A3

Designated state(s): AU DK FI HU JP NO US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE FR GB IT LU NL SE

WWE Wipo information: entry into national phase

Ref document number: 1988904209

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1988904209

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1988904209

Country of ref document: EP