WO1988005993A3 - Aminopeptidase for removing n-terminal methionine from proteins and proteins prepared therefrom - Google Patents

Aminopeptidase for removing n-terminal methionine from proteins and proteins prepared therefrom Download PDF

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Publication number
WO1988005993A3
WO1988005993A3 PCT/EP1988/000096 EP8800096W WO8805993A3 WO 1988005993 A3 WO1988005993 A3 WO 1988005993A3 EP 8800096 W EP8800096 W EP 8800096W WO 8805993 A3 WO8805993 A3 WO 8805993A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
aminopeptidase
peptidase
strains
terminal methionine
Prior art date
Application number
PCT/EP1988/000096
Other languages
French (fr)
Other versions
WO1988005993A2 (en
Inventor
Charles G Miller
Rao Movva
Paul Wingfield
Gonzola J Mazzei
Original Assignee
Biogen Nv
Charles G Miller
Rao Movva
Paul Wingfield
Gonzola J Mazzei
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Nv, Charles G Miller, Rao Movva, Paul Wingfield, Gonzola J Mazzei filed Critical Biogen Nv
Priority to JP63504077A priority Critical patent/JPH03502400A/en
Publication of WO1988005993A2 publication Critical patent/WO1988005993A2/en
Publication of WO1988005993A3 publication Critical patent/WO1988005993A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Abstract

An aminopeptidase, peptidase M, that removes only N-terminal methionine, strains of microorganisms that produce the aminopeptidase, and polypeptides treated with the aminopeptidase. Peptidase M may be used to cleave polypeptides proteins in vitro, or the DNA of the strains that produce Peptidase M may be used to transform a recombinant host to prepare mature proteins in vivo. The strains that produce Peptidase M may themselves be used as recombinant hosts. Methods for isolating peptidase M and methods for isolating strains that overproduce peptidase M are also disclosed.
PCT/EP1988/000096 1987-02-10 1988-02-09 Aminopeptidase for removing n-terminal methionine from proteins and proteins prepared therefrom WO1988005993A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP63504077A JPH03502400A (en) 1988-02-09 1988-02-09 Aminopeptidases that remove N-terminal methionine from proteins and proteins prepared thereby

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8703015 1987-02-10
GB878703015A GB8703015D0 (en) 1987-02-10 1987-02-10 Aminopeptidase

Publications (2)

Publication Number Publication Date
WO1988005993A2 WO1988005993A2 (en) 1988-08-25
WO1988005993A3 true WO1988005993A3 (en) 1988-11-17

Family

ID=10612035

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1988/000096 WO1988005993A2 (en) 1987-02-10 1988-02-09 Aminopeptidase for removing n-terminal methionine from proteins and proteins prepared therefrom

Country Status (4)

Country Link
EP (1) EP0366662A1 (en)
AU (1) AU1719988A (en)
GB (1) GB8703015D0 (en)
WO (1) WO1988005993A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5013662A (en) * 1985-09-20 1991-05-07 Cetus Corporation Bacterial methionine n-terminal peptidase
AU4057889A (en) * 1988-08-26 1990-03-23 General Hospital Corporation, The Isolation, purification, and characterization of the methionine-specific aminopeptidases: mas i and mas x
AU4188189A (en) * 1988-09-13 1990-04-02 General Hospital Corporation, The Isolation, purification, and characterization of the aminopeptidases: mas ii and mas iii
AU4221089A (en) * 1988-09-13 1990-04-02 General Hospital Corporation, The Isolation, purification, and characterization of the aminopeptidases: ap2, ap1, and apx
WO1990002813A1 (en) * 1988-09-13 1990-03-22 The General Hospital Corporation Isolation, purification, and characterization of the aminopeptidases: ap1 and ap122
KR970010135B1 (en) * 1993-06-17 1997-06-21 주식회사 엘지화학 Novel aminopeptidase isolated from streptococcus thermonitrificans

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986001229A1 (en) * 1984-08-16 1986-02-27 Bio-Technology General Corp. Method of removing n-terminal amino acid residues from eucaryotic polypetide analogs and polypeptides produced thereby
EP0204527A1 (en) * 1985-06-04 1986-12-10 Takeda Chemical Industries, Ltd. Removal of the N-terminal methionine from methionylproteins
EP0219237A1 (en) * 1985-09-20 1987-04-22 Cetus Corporation A bacterial methionine N-terminal peptidase, its preparation and application

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986001229A1 (en) * 1984-08-16 1986-02-27 Bio-Technology General Corp. Method of removing n-terminal amino acid residues from eucaryotic polypetide analogs and polypeptides produced thereby
EP0204527A1 (en) * 1985-06-04 1986-12-10 Takeda Chemical Industries, Ltd. Removal of the N-terminal methionine from methionylproteins
EP0219237A1 (en) * 1985-09-20 1987-04-22 Cetus Corporation A bacterial methionine N-terminal peptidase, its preparation and application

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Biological Abstracts, RRM/34117064, (Philadelphia, US), A. Ben-Bassat et al.: "N-terminal processing of proteins by methionine aminopeptidase in Escherichia coli" & Abstracts of the Annual Meeting of the American Society for microbiology, vol. 86, 1986 (US), see page 208, abstract no. K-89 *
Biotechnology, vol. 5, no. 8, August 1987, (New York, New York, US), S. Nakagawa et al.: "Enzymatic cleavage of amino terminal methionine from recombinant human interleukin 2 and growth hormone by aminopeptidase M", pages 824-827 *
Journal of Bacteriology, vol. 169, no. 2, February 1987, American Society for Microbiology (US), A. Ben-Bassat et al.: "processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure", pages 751-757 *
Proc. Natl. Acad Sci. USA, vol. 84, May 1987 (Washington, US), C.G. Miller et al.: "N-terminal methionine-specific peptidase in Salmonella typhimurium", pages 2718-2722 *

Also Published As

Publication number Publication date
GB8703015D0 (en) 1987-03-18
AU1719988A (en) 1988-09-14
EP0366662A1 (en) 1990-05-09
WO1988005993A2 (en) 1988-08-25

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