WO1988002851A3 - Chambre froide pour realiser des coupes minces a temperature reduite - Google Patents

Chambre froide pour realiser des coupes minces a temperature reduite Download PDF

Info

Publication number
WO1988002851A3
WO1988002851A3 PCT/EP1987/000596 EP8700596W WO8802851A3 WO 1988002851 A3 WO1988002851 A3 WO 1988002851A3 EP 8700596 W EP8700596 W EP 8700596W WO 8802851 A3 WO8802851 A3 WO 8802851A3
Authority
WO
WIPO (PCT)
Prior art keywords
tank
nitrogen
liquid nitrogen
filling
chamber
Prior art date
Application number
PCT/EP1987/000596
Other languages
English (en)
French (fr)
Other versions
WO1988002851A2 (fr
Inventor
Hellmuth Sitte
Helmut Haessig
Klaus Neumann
Original Assignee
Hellmuth Sitte
Helmut Haessig
Klaus Neumann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hellmuth Sitte, Helmut Haessig, Klaus Neumann filed Critical Hellmuth Sitte
Publication of WO1988002851A2 publication Critical patent/WO1988002851A2/de
Publication of WO1988002851A3 publication Critical patent/WO1988002851A3/de

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
    • F25D3/10—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation

Landscapes

  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Mechanical Engineering (AREA)
  • Thermal Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Combustion & Propulsion (AREA)
  • Sampling And Sample Adjustment (AREA)
PCT/EP1987/000596 1986-10-14 1987-10-13 Chambre froide pour realiser des coupes minces a temperature reduite WO1988002851A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ATA2731/86 1986-10-14
AT0273186A AT390512B (de) 1986-10-14 1986-10-14 Kuehlkammer zum herstellen von duennen schnitten bei reduzierter temperatur

Publications (2)

Publication Number Publication Date
WO1988002851A2 WO1988002851A2 (fr) 1988-04-21
WO1988002851A3 true WO1988002851A3 (fr) 1988-06-02

Family

ID=3539403

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1987/000596 WO1988002851A2 (fr) 1986-10-14 1987-10-13 Chambre froide pour realiser des coupes minces a temperature reduite

Country Status (5)

Country Link
US (1) US5070935A (de)
EP (1) EP0327597A1 (de)
JP (1) JPH02501005A (de)
AT (1) AT390512B (de)
WO (1) WO1988002851A2 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4012600C2 (de) * 1989-05-26 1998-05-14 Leica Ag Mikrotom
US5275007A (en) * 1992-07-14 1994-01-04 Minnesota Valley Engineering, Inc. Cryogenic dewar level sensor and flushing system
USD383548S (en) * 1994-09-23 1997-09-09 Leica Instruments Gmbh Rotational microtome
US5533342A (en) * 1995-02-08 1996-07-09 Gordon; Abe Semi-automated cryostat
CN1116572C (zh) * 1996-02-06 2003-07-30 石塚电子株式会社 结霜探测器
DE19640044A1 (de) * 1996-09-30 1998-04-02 Microm Laborgeraete Gmbh Kryostatmikrotom
EP1066488A1 (de) * 1998-02-26 2001-01-10 Lucid, Inc. Konfokales mikroskop zur erleichterung der kryochirurgie von geweben
AT406309B (de) * 1998-09-01 2000-04-25 Leica Mikrosysteme Ag Vorrichtung zur regelung der temperaturen in einer kühlkammer für ein mikrotom
DE19911163C1 (de) * 1999-03-12 2000-07-20 Leica Microsystems Mikrotom
JP2005098702A (ja) * 1999-10-07 2005-04-14 Seitai Kagaku Kenkyusho:Kk 生物組織試料作製方法および器具
DE10318026C5 (de) * 2003-04-19 2015-11-12 Leica Biosystems Nussloch Gmbh Kryostat mit Beleuchtungseinrichtung
DE10346996B3 (de) * 2003-10-07 2005-05-25 Leica Mikrosysteme Gmbh Mikrotom mit Kühlkammer
DE20318094U1 (de) * 2003-11-22 2004-02-12 Leica Microsystems Nussloch Gmbh Kryostat mit einer Wärmeplatte
US7437984B2 (en) * 2004-12-10 2008-10-21 Boeckeler Instruments Inc. Microtome and ultramicrotome with improved carrier arm
DE102005037060B4 (de) * 2005-08-05 2013-10-17 Leica Mikrosysteme Gmbh Verfahren zur Verminderung der Luftfeuchtigkeit in der Umgebung der Kühlkammer einer Einrichtung zum Schneiden von Proben
US20070095668A1 (en) * 2005-10-31 2007-05-03 Phillip Pendleton Liquid nitrogen level control system
JP6189031B2 (ja) * 2012-11-20 2017-08-30 株式会社ワイエイシイダステック 生体試料の切断装置及び切断方法並びに細胞観察方法
JP6829075B2 (ja) * 2014-05-12 2021-02-10 アメリカ合衆国 ブロック面イメージング用の小型連続セクショニングミクロトーム
AT522172B1 (de) * 2019-04-23 2020-09-15 Univ Linz Vorrichtung zur Lagerung eines Objekthalters

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1170796A (en) * 1967-02-17 1969-11-19 Shandon Scient Ind Ltd Improvements in or relating to Microtomes
DE2246853A1 (de) * 1971-11-09 1973-05-24 Reichert Optische Werke Ag Schalenbauweise fuer mikrotome und ultramikrotome
DE2328298A1 (de) * 1972-09-06 1974-03-28 Reichert Optische Werke Ag Kuehlkammer fuer ultramikrotome
US4284894A (en) * 1979-02-17 1981-08-18 C. Reichert Optische Werke, Ag Cold chamber for the working objects for microscopic and electron microscopic investigations

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3234457C2 (de) * 1982-09-17 1984-09-20 C. Reichert Optische Werke Ag, Wien Kühlbad zum raschen Abkühlen von Proben, insbesondere zur Kryofixation biologischer Objekte für eine nachfolgende licht- oder elektronenoptische Untersuchung

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1170796A (en) * 1967-02-17 1969-11-19 Shandon Scient Ind Ltd Improvements in or relating to Microtomes
DE2246853A1 (de) * 1971-11-09 1973-05-24 Reichert Optische Werke Ag Schalenbauweise fuer mikrotome und ultramikrotome
DE2328298A1 (de) * 1972-09-06 1974-03-28 Reichert Optische Werke Ag Kuehlkammer fuer ultramikrotome
US4284894A (en) * 1979-02-17 1981-08-18 C. Reichert Optische Werke, Ag Cold chamber for the working objects for microscopic and electron microscopic investigations

Also Published As

Publication number Publication date
JPH02501005A (ja) 1990-04-05
EP0327597A1 (de) 1989-08-16
US5070935A (en) 1991-12-10
AT390512B (de) 1990-05-25
WO1988002851A2 (fr) 1988-04-21
ATA273186A (de) 1989-10-15

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