WO1988000695A1 - Method of detecting a substance using enzymatically-induced decomposition of dioxetanes - Google Patents

Method of detecting a substance using enzymatically-induced decomposition of dioxetanes Download PDF

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Publication number
WO1988000695A1
WO1988000695A1 PCT/US1987/000554 US8700554W WO8800695A1 WO 1988000695 A1 WO1988000695 A1 WO 1988000695A1 US 8700554 W US8700554 W US 8700554W WO 8800695 A1 WO8800695 A1 WO 8800695A1
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WO
WIPO (PCT)
Prior art keywords
enzyme
group
dioxetane
cleavable
cleavable group
Prior art date
Application number
PCT/US1987/000554
Other languages
English (en)
French (fr)
Inventor
Irena Y. Bronstein
Original Assignee
Quest Systems, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=33565664&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1988000695(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Quest Systems, Inc. filed Critical Quest Systems, Inc.
Priority to AT87902259T priority Critical patent/ATE97488T1/de
Priority to DE87902259T priority patent/DE3788203T2/de
Priority to BR8707399A priority patent/BR8707399A/pt
Publication of WO1988000695A1 publication Critical patent/WO1988000695A1/en
Priority claimed from CA000604507A external-priority patent/CA1340590C/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/203—Monocyclic carbocyclic rings other than cyclohexane rings; Bicyclic carbocyclic ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D321/00—Heterocyclic compounds containing rings having two oxygen atoms as the only ring hetero atoms, not provided for by groups C07D317/00 - C07D319/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • C07F9/12—Esters of phosphoric acids with hydroxyaryl compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
    • C07F9/6551—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a four-membered ring
    • C07F9/65512—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a four-membered ring condensed with carbocyclic rings or carbocyclic ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6564—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having phosphorus atoms, with or without nitrogen, oxygen, sulfur, selenium or tellurium atoms, as ring hetero atoms
    • C07F9/6571—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having phosphorus atoms, with or without nitrogen, oxygen, sulfur, selenium or tellurium atoms, as ring hetero atoms having phosphorus and oxygen atoms as the only ring hetero atoms
    • C07F9/6574—Esters of oxyacids of phosphorus
    • C07F9/65742—Esters of oxyacids of phosphorus non-condensed with carbocyclic rings or heterocyclic rings or ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/26—Acyclic or carbocyclic radicals, substituted by hetero rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
PCT/US1987/000554 1986-07-24 1987-03-11 Method of detecting a substance using enzymatically-induced decomposition of dioxetanes WO1988000695A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AT87902259T ATE97488T1 (de) 1986-07-24 1987-03-11 Verfahren zum nachweis einer verbindung unter verwendung des enzymatisch induzierten zerfalls von dioxethanen.
DE87902259T DE3788203T2 (de) 1986-07-24 1987-03-11 Verfahren zum nachweis einer verbindung unter verwendung des enzymatisch induzierten zerfalls von dioxethanen.
BR8707399A BR8707399A (pt) 1986-07-24 1987-03-11 Metodo de detectar uma substancia usando decomposicao enzimaticamente induzida de dioxetanos

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US88982386A 1986-07-24 1986-07-24
US889,823 1986-07-24
CA000604507A CA1340590C (en) 1986-07-24 1989-06-30 Chemiluminescence enhancement

Publications (1)

Publication Number Publication Date
WO1988000695A1 true WO1988000695A1 (en) 1988-01-28

Family

ID=33565664

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1987/000554 WO1988000695A1 (en) 1986-07-24 1987-03-11 Method of detecting a substance using enzymatically-induced decomposition of dioxetanes

Country Status (6)

Country Link
US (6) US5866389A (US06913897-20050705-C00021.png)
EP (1) EP0275260B1 (US06913897-20050705-C00021.png)
JP (1) JP2506396B2 (US06913897-20050705-C00021.png)
BR (1) BR8707399A (US06913897-20050705-C00021.png)
DE (1) DE3788203T2 (US06913897-20050705-C00021.png)
WO (1) WO1988000695A1 (US06913897-20050705-C00021.png)

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EP0377729A1 (en) * 1988-06-30 1990-07-18 Tropix Inc IMPROVED METHODS OF USING CHEMOLUMINESCENT 1,2-DIOXETANES.
EP0401001A2 (en) * 1989-05-31 1990-12-05 Chiron Corporation Chemiluminescent doubletriggered 1,2-dioxetanes
US4978614A (en) * 1988-10-26 1990-12-18 Tropix, Inc. Method of detecting a substance using enzymatically-induced decomposition of dioxetanes
DE3921609A1 (de) * 1989-06-27 1991-01-03 Tropix Inc Chemilumineszenzsteigerung
WO1991003479A1 (en) * 1989-09-06 1991-03-21 Tropix, Inc. Synthesis of stable, water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor
US5004565A (en) * 1986-07-17 1991-04-02 The Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
EP0420186A2 (en) * 1989-09-29 1991-04-03 Fujirebio Inc. Immunoassay using magnetic particle
EP0440193A2 (en) * 1990-01-31 1991-08-07 Fujirebio Inc. Immunochemical assay method with plural items
US5094939A (en) * 1988-07-19 1992-03-10 Fujirebio, Inc. Chemiluminescence assays using stabilized dioxetane derivatives
US5109124A (en) * 1988-06-01 1992-04-28 Biogen, Inc. Nucleic acid probe linked to a label having a terminal cysteine
WO1993003380A1 (en) * 1991-07-31 1993-02-18 E.I. Du Pont De Nemours And Company Process for enhancing the chemiluminescence of enzyme-triggered 1,2-dioxetanes and uses therefor
US5225584A (en) * 1989-09-06 1993-07-06 Tropix, Inc. Synthesis of stable water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor
DE4304728A1 (de) * 1993-02-13 1994-08-18 Igor Dr Popov Verfahren und Testbesteck zur Bestimmung von Ascorbinsäure in biologischen Proben
US5340716A (en) * 1991-06-20 1994-08-23 Snytex (U.S.A.) Inc. Assay method utilizing photoactivated chemiluminescent label
US5362916A (en) * 1989-09-06 1994-11-08 Tropix, Inc. Synthesis of mercaptaryl or hydroxyaryl enol ether alkali metal salts
US5393469A (en) * 1992-03-20 1995-02-28 Lumigen, Inc. Polymeric phosphonium salts providing enhanced chemiluminescence from 1,2-dioxetanes
WO1995018530A1 (en) * 1994-01-10 1995-07-13 Hemasure Inc. Inactivation of viruses present in blood components using chemically-activated compounds
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USRE36536E (en) * 1986-07-24 2000-01-25 Tropix. Inc. Method of detecting a substance using enzymatically-induced decomposition of dioxetanes
US6165800A (en) * 1997-05-30 2000-12-26 Bayer Corporation Chemiluminescent energy transfer conjugates and their uses as labels in binding assays
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US8784734B2 (en) 2010-05-20 2014-07-22 Abbott Laboratories Reusable sheaths for separating magnetic particles
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US6022964A (en) 1989-07-17 2000-02-08 Tropix, Inc. Chemiluminescent 1,2-dioxetanes
US6245928B1 (en) * 1995-07-31 2001-06-12 Lumigen, Inc. Water soluble tri-substituted 1,2-dioxetane compounds having increased storage stability, synthetic processes and intermediates
DE19536166C1 (de) 1995-09-29 1997-03-06 Siegfried Dr Krell Verfahren zur Bestimmung von Antikörpern gegen Treponema pallidum (Syphilis)
JP3182527B2 (ja) * 1996-09-03 2001-07-03 株式会社ヤトロン 化学発光の測定方法及びキット
CA2370520A1 (en) * 1999-04-16 2000-10-26 Komandoor Elayavalli Achyuthan Viral detection method using viral encoded enzymes and chemiluminescent substrates
AU3958999A (en) * 1999-06-01 2000-12-18 Kyokuto Pharmaceutical Industrial Co., Ltd. Method for assaying anti-gad antibody and kit
US7432063B2 (en) 2002-02-14 2008-10-07 Kalobios Pharmaceuticals, Inc. Methods for affinity maturation
US7390670B2 (en) 2003-02-20 2008-06-24 Lumigen, Inc. Signalling compounds and methods for detecting hydrogen peroxide
US20050019955A1 (en) * 2003-07-23 2005-01-27 Dahl Jeremy E. Luminescent heterodiamondoids as biological labels
US8518658B1 (en) 2009-04-27 2013-08-27 University Of South Florida ATP-bioluminescence immunoassay

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Cited By (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004565A (en) * 1986-07-17 1991-04-02 The Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
USRE36536E (en) * 1986-07-24 2000-01-25 Tropix. Inc. Method of detecting a substance using enzymatically-induced decomposition of dioxetanes
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BR8707399A (pt) 1988-09-13
US6913897B2 (en) 2005-07-05
JPH04500901A (ja) 1992-02-20
US20030175838A1 (en) 2003-09-18
US5869705A (en) 1999-02-09
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US5866389A (en) 1999-02-02
EP0275260A1 (en) 1988-07-27
EP0275260B1 (en) 1993-11-18
JP2506396B2 (ja) 1996-06-12
DE3788203T2 (de) 1994-03-31
US6113816A (en) 2000-09-05
US20020102624A1 (en) 2002-08-01
US6322727B1 (en) 2001-11-27
DE3788203D1 (de) 1993-12-23

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