WO1986001410A1 - Antibody-metal ion complexes - Google Patents

Antibody-metal ion complexes

Info

Publication number
WO1986001410A1
WO1986001410A1 PCT/US1985/001556 US8501556W WO8601410A1 WO 1986001410 A1 WO1986001410 A1 WO 1986001410A1 US 8501556 W US8501556 W US 8501556W WO 8601410 A1 WO8601410 A1 WO 8601410A1
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
metal ion
chelator
acid
fragment
Prior art date
Application number
PCT/US1985/001556
Other languages
English (en)
French (fr)
Inventor
Richard Charles Siegel
Chyi Lee
Vernon Leon Alvarez
John Dennis Rodwell
Thomas Joseph Mckearn
John Walter Frank Goers
Original Assignee
Cytogen Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/646,328 external-priority patent/US4741900A/en
Application filed by Cytogen Corporation filed Critical Cytogen Corporation
Priority to JP60503820A priority Critical patent/JPH0651720B2/ja
Publication of WO1986001410A1 publication Critical patent/WO1986001410A1/en
Priority to DK195186A priority patent/DK195186D0/da

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/08—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier
    • A61K49/10—Organic compounds
    • A61K49/14—Peptides, e.g. proteins
    • A61K49/16—Antibodies; Immunoglobulins; Fragments thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
    • A61K49/08—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier
    • A61K49/085—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier conjugated systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A61K51/1093—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody conjugates with carriers being antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/534—Production of labelled immunochemicals with radioactive label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2121/00—Preparations for use in therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2123/00—Preparations for testing in vivo

Definitions

  • the present invention relates to the general area of antibody systems capable of delivering metal ions to target sites _in vivo or _in v i tro .
  • the metal ions are radio isotopes or another detectible ion
  • the Q antibody systems are capable of being identified and/or detected owing to the radioisotope or other detectible ion attached to the antibody. More particularly, the invention
  • SUBSTITUTE SHEET is directed to water-soluble antibody-metal ion complexes comprising metal ions attached by coordinate bonding via a compatible chelator to an antibody molecule which substantially retains the immuno specificity and - immunore activity of the original antibody.
  • the invention also relates to several methods for preparing such antibody- metal ion complexes as well as antibody-chelator ' conjugates which are useful in preparing such complexes.
  • the antibody-metal ion complexes of the invention 0 may be used in a variety of ij ⁇ _ vitro or j_ vivo applications involving detection or delivery of a labeled antibody or a metal ion, including, but not limited to, immunological assays, imaging of specific tissues and delivery of metal ions and/or radioisotopes to specific tissue sites. 5
  • A- number of agents have been utilized as carrier molecules with limited success in imaging systems.
  • the carrier should be non- toxic and target site specific.
  • Radiopharmaceutical techniques currently used in non- invasive _in vivo imaging methods are based upon the ability of the target organ to remove the radiopharma ⁇ ceutical label from the circulation. These techniques utilize various substances to deliver radioactive compounds 0 to desired target; such substances include substrates, substrate analogs, ligands, hormones, ra ionuclides , bifunctional chelates (linker groups containing a chelator at one end which is able to bind a heavy metal or radioisotope 5
  • Monoclonal antibodies produced by the hybridoma 5 technique of Kohler and Milstein (1975, Nature 256: 495-497; 1976, Eur. J. Immunol. 6_: 511-519) or related techniques provide distinct advantages for use as carriers for imaging systems.
  • monoclonal antibodies bind to only one molecular site (i .e ⁇ , an epitope) with specific binding 0 constants.
  • Second, such antibodies are homogeneous and thus are purified with relative ease.
  • monoclonal antibodies can be made in large quantities by particular hybridoma cell lines .
  • SUBSTITUTE SHEET including: oncofetal antigens, placental antigens, oncogenic or tumor virus-associated antigens, tissue-associated antigens, organ-associated antigens, ecotopic hormones, normal antigens and variants thereof.
  • Goldenberg , et al have demonstrated clinical detection of tumors in human patients using 1311-radiolabeled antibody specific for care inoembr yon ic antigen (1978, New Eng. J. Med . 298 : 1384-1388; see also disclosures in United States Patent No. 4,331,647 issued to Goldenberg, United States Patent No. 3,927,193 to Hansen, et al.) .
  • the methods utilized by Goldenberg, et al . required either repeated injection of other radioactive materials or single injection of mixtures of radioactive antibody in order to attain sufficient resolution to distinguish tumors from non-target areas.
  • Iodine, Indium, and Copper have been attached to monoclonal or polyclonal antibodies for use in detection of tumors in vivo in animal models.
  • Vincour (1984, Diagnostic Imaging, Feb. 1984, pp. 56-61) reviews the problems experienced with such radiolabeled antibodies, noting especially the need for development of ra iolabels which
  • a common feature of prior art in vivo images generated either with radioactive metal chelates or 131 Iodine 5 attached to antibodies is non-targeted localization in organs such as liver or spleen (or thyroid for I) .
  • Such non ⁇ specific localization results in images which do not accurately delineate the position, orientation or size of the intended target. .Frequently, "background subtraction" of 0 non-specif ically localized radioactivity does not adequately compensate .
  • radio immuno imaging The primary distinctions between the materials used 5 in radio immuno imaging and radioimmunotherapy are the amount and type the radiation employed.
  • radiation is employed to kill target cells while causing minimal toxic ity to non- targeted cells.
  • the radiation ideally has high energy and a short travel range in tissue. 0
  • SHEET which provide limited success with _in vivo imaging may not be suitable at all for therapeutic applications.
  • a metal ion is. attached to an antibody or antibody fragment directed against a target antigen. Attachment is accomplished using chelator s (i .e . , compounds that are able to donate electrons and combine by coordinate bonding with a metal ion to form structures termed chelates or chelation complexes) between the metal ion and the antibody. After formation of antibody-metal ion complexes, non-spec if ically attached metal ions should be removed. According to the preferred .embodiment of the present invention then, removal of non-specif ically attached metal ions from the complexes is accomplished using a high performance liquid molecular sieve chroma tography system.
  • the invention contemplates site selective attachment of compatible chelators to those areas of antibodies or antibody fragments which are not a part of nor directly involved with the antigenic site of the molecule.
  • the antibody conjugate formed has substantially the same immunoreactivity
  • b _ compatible chelators containing an amine group selected from the group consisting of primary amine, hydrazine , hydrazide, hydroxylamine , phenylhydrazine , semicarbazide and thiosemicarbazide groups are attached directly to the oxidized carbohydrate moieties of the antibodies or antibody fragments according to methods of attachment described 0 herein .
  • compatible chelators having certain reactive groups capable of reaction with a sulfhydryl group may be attached to 5 reduced antibodies or reduced (Fab') 7 fragments.
  • FIG. 1 schematically represents the elution pattern of Indium-CYT-015-p-aminoaniline- ie thyl enetri ine pen taace tic acid ( In-CYT-015-ADTPA) on 5 high pressure liquid gel permeation chromatography (see Section 6.2.2) .
  • FIG. 2 represents autoradiographic images of Brown Norway (BN) tumor-bearing nude mice injected with Indium- 0 labeled anti-Brown Norway (BN) major histocompatibility complex (MHC) antibody-metal ion complex, In-CYT-015-
  • BN Brown Norway
  • MHC major histocompatibility complex
  • ADTPA Images A, B, C and D were taken 24, 24, 48 and 72 hours after injection, respectively (see Section 8.1) .
  • JTS SHEET FIG. 3 presents autoradiographic images of control
  • FIG. 4 represents an autoradiographic image of control non- tumor- bearing nude mice injected with llllndium- labeled anti-BN MHC antibody-metal ion complex,- In-CYT-
  • FIG. 5 represents an autora iographic image of a
  • the instant invention concerns antibody-metal ion complexes prepared by attaching a metal ion to an antibody or antibody fragment directed against a target antigen.
  • Compatible chelators capable of coordinate bonding with a metal ion are utilized to attach the metal ions to the antibody or antibody fragment. Such chelators are selectively attached to those areas of antibodies or antibody fragments which are not a part of nor directly involved with the antigenic site of the molecule.
  • the invention concerns methods for preparing antibody-metal ion complexes, comprising: (a) reacting an antibody or antibody fragment with an oxidizing agent to form an aldehyde group in the carbohydrate moiety of the antibody or antibody fragment;
  • the first part would produce an antibody-chelator conjugate, which may be considered an intermediate in the production of the final antibody-metal ion complex.
  • Such antibody-chelator conjugates may be stored for later combination with the particular metal ion -of interest.
  • the first part of the two part method would involve steps (a) and (b) above to form the intermediate antibody-chelator conjugate.
  • the second part possibly at a later point in time, would involve complexing the antibody-chelator conjugate with a metal ion to produce the final antibody-metal ion complex.
  • Such antibody-metal ion complexes can also be made by alternate methods, as, for example, by first coordinately bonding the compatible chelator to the metal ion, and then reacting the antibody or antibody fragment with the chelator-metal ion complex to form the antibody-metal ion complex.
  • the invention further includes a method for preparing an antibody-metal ion complex, comprising:
  • chelator-metal ion complex comprising a compatible chelator containing an amine group selected from the group consisting of primary amine, hydrazine, hydrazide, hydroxylamine , phenylhydrazine , semicarbazide and thiosemicarbazide groups coordinately bound to a metal ion, to form an antibody-metal ion complex having substantially the same immunoreactivity and immunospecificity as the unconjugated antibody or antibody fragment.
  • the compatible chelator is coordinately bound to the metal ion prior, to covalent attachment of the chelator to the antibody or antibody fragment .
  • the invention is also directed to intermediates and final products of the above-described methods. More specifically, this invention is directed to antibody-chelator conjugates comprising a compatible chelator attached through a covalent bond to a carbohydrate moiety of an oxidized antibody or antibody fragment, said antibody-chelator
  • the invention concerns antibody- metal ion complexes comprising an antibody-chelator conjugate comprising a compatible chelator attached through a covalent bond to a carbohydrate moiety of an oxidized antibody or antibody fragment, said antibody-chelator conjugate coordinately bound through said compatible chelator to a metal ion to form an antibody-metal ion complex having substantially the same immunoreactivity and immunospecificity as the unconjugated antibody or antibody fragment.
  • antibody- chelator conjugates and antibody-metal ion complexes in which the compatible chelator is attached to a sulfur atom of a reduced antibody or Fab 1 fragment.
  • a method for preparing an antibody-metal ion complex comprising:
  • the same antibody-metal ion complexes can be made by another method, comprising:
  • the product antibody-metal ion complex comprises an antibody-chelator conjugate comprising a compatible chelator attached through a covalent bond to a sulfur atom of a reduced antibody or Fab' fragment, said antibody-chelator conjugate coordinately bound through said compatible chelator to a metal ion to form an antibody-metal ion complex having substantially the same immunoreactivity and immunospecificity as the unconjugated antibody or ( Fab ' ) _ fragment.
  • the invention contemplates the intermediate antibody-chelator conjugate which comprises a compatible chelator attached through a covalent bond to a sulfur atom of a reduced antibody or Fab' fragment, said
  • the purified complexes of the invention contain metal ion chelated to the chelator, but are substantially free of metal ion adventitiously bound to antibody, that is, metal ion associated with the antibody protein or carbohydrate and not complexed to the. chelator.
  • the purification can be accomplished by any suitable purification method, including but not limited to high performance gel permeation liquid chroma tography .
  • This purification step has the additional benefit of removing undesirable aggregates of antibodies prior to administration. Any such antibody aggregates or denatured antibodies would be taken up by the reticuloendothelial system for removal, and this transport away from the target site or specific tissue to be imaged would diminish the degree of localization or the quality of the image.
  • the antibody-metal ion complexes of the invention are ideally suited for high-resolution tissue imaging in vivo .
  • imaging of specific tissue involves administering to a human an effective amount of an antibody-
  • EET etal ion complex wherein said antibody-metal ion complex is immunoreactive with and immunospecific for an antigenic determinant of said specific tissue and substantially non- immunoreactive with and non- immuno specific for non-specific tissue and said antigenic determinant is not found in substantial amount in non-specific tissue.
  • this method can be used to localize specific tissue with a minimum of "background" dispersal of metal ion.
  • Such methods comprise administering a therapeutically effective amount of an antibody-metal ion complex of the invention, said antibody-metal ion complex being immunoreactive with and immunospecific for a target site associated with said cellular disorder and substantially non-immunoreactive with and non-immunospecif ic for tissue not associated with said cellular disorder, and wherein the metal ion emits cytotoxic beta particles or alpha particles.
  • the complexes of the invention may also be used in methods for testing for antigen, which involve, for example, (a) mixing an antibody-metal ion complex with a sample suspected of containing a particular antigen, the antibody-metal ion complex being immunoreactive with and immunospecific for said antigen, and (b) detecting any interaction of said antibody-metal ion complex with any antigen in the sample.
  • antibodies directed against any antigen or hapten may be used.
  • monoclonal 5 antibodies offer several advantages. Each monoclonal antibody is specific for one antigenic determinant. Additionally, large amounts of each monoclonal antibody can be produced. 0
  • Antibodies used in the present invention may be directed against any target, e.g., tumor, bacterial, f ngal, viral, parasitic, mycoplasmal , histocompatability , differentiation and other cell membrane antigens, pathogen surface antigens, toxins, enzymes, allergens, drugs and any 5 biologically active molecules.
  • target e.g., tumor, bacterial, f ngal, viral, parasitic, mycoplasmal , histocompatability , differentiation and other cell membrane antigens, pathogen surface antigens, toxins, enzymes, allergens, drugs and any 5 biologically active molecules.
  • Drugs of particular interest are opio.ids, amphetamines, barbiturates, steroids, catecholamines , dilantin, theophylline , histamine, cannabinoids , and the 0 like.
  • drugs of particular interest are opio.ids, amphetamines, barbiturates, steroids, catecholamines , dilantin, theophylline , histamine, cannabinoids , and the 0 like.
  • Immunoglobulins which may be used as carriers include: certain classes of antibodies such as IgA, IgD, IgE, IgM; certain classes of 30
  • immunoglobulins e.g., half antibody molecules (a single heavy :light chain pair) , or Fab,
  • the Fab' fragments of IgG immunoglobulins are obtained by cleaving the antibody molecule with pepsin (resulting in a bivalent fragment, (Fab') 2 ) or with papain (resulting in 2 univalent fragments, 2 Fab) . Parham, 1983, J. Immunol. 131 : 2895-2902;
  • the bivalent (Fab') 2 fragment can be split by mild reduction of one or a few disulfide bonds to yield univalent Fab 1 0 fragments.
  • the " Fab and (Fab') 2 fragments are smaller than a whole antibody molecule and, therefore, permeate the target site or tissue more easily. This may offer an advantage for in vivo imaging since conjugates will more readily penetrate
  • ._ in vivo sites e.g., tumor masses, infection sites, etc.
  • an _in vivo site (such as a tumor) may be imaged in a pregnant female without exposing
  • compatible chelators which have multiple sites for chelation of metal ions.
  • a single covalent attachment to an antibody or antibody fragment would result in a conjugate capable of binding metal ion at a number of sites.
  • Radioactive complexes of such conjugates would have high specific radioactivity, that is, high radioactivity per antibody molecule.
  • higher specific radioactivity can be achieved by attachment of a single site compatible chelator at a plurality of sites of the antibody or antibody fragment.
  • This plurality of sites may be introduced into the antibody or antibody fragment by either of two methods.
  • the functional sites of the branched linker may be aldehyde or sulfhydryl groups, or may be any chemical site to which compatible chelators may be attached. Still higher specific radioactivities may be obtained by combining these two approaches, that is, attaching multiple site compatible chelators at several sites on the antibody or antibody fragment .
  • Compatible chelators include, but are
  • UTE SHEET not limited to, derivatives of iethylenetriamine-pentaacetic acid, ethylenediaminetetraacetic acid (EDTA) , dimercaptosuccinic acid, 2,3-dimercaptopropanesulfonic acid, etallothioein and cryptates, such as those recently described by Gansow, et al. (1981, J. Heterocyclic Chem. 18 : 297) .
  • EDTA ethylenediaminetetraacetic acid
  • dimercaptosuccinic acid dimercaptosuccinic acid
  • 2,3-dimercaptopropanesulfonic acid 2,3-dimercaptopropanesulfonic acid
  • etallothioein and cryptates such as those recently described by Gansow, et al. (1981, J. Heterocyclic Chem. 18 : 297) .
  • compatible chelators are utilized to attach metal ions to antibody molecules.
  • suitable compatible chelators for reaction with oxidized antibodies or oxidized antibody fragments include those containing an amine selected from the group consisting of primary amine, hydrazine, hydrazide, hydroxyl amine , phenylhydrazine , semicarbazide and thiosemicarbazide groups .
  • Such reactive functional groups may. exist as part of the structure of the chelator, or may be introduced by suitable chemistry on chelators not containing such " groups .
  • diethylenetriaminepentaacetic acid lacks the appropriate amine group for facile attachment to oxidized carbohydrate.
  • chemical modification can produce a variety of suitable derivatives, such as amine-containing derivatives of mixed anhydrides of DTPA including, but not limited to p-aminoanili.ne-DTPA, hydrazide-DTPA, phenylhydrazide-DTPA, hydroxylamine-DTPA, sem ic ar b azide-DTPA, thio semjLc ar b a z i d e- DTPA , polyethylene imine-DTPA, p-phenylenediamine- DTPA, DTPA mono [ (4-aminophenyl) methyl] amide and amino acid-containing derivatives of DTPA, including, but not limited to ⁇ -N-DTPA-
  • suitable compatible chelators for attachment to reduced antibody or antibody fragments include chelators having certain reactive groups capable of reaction with a sulfhydryl group of a reduced antibody or Fab' fragment.
  • reactive groups include, but are not limited to, reactive haloalkyl groups (including, for example, haloacetyl groups), p- ercur ibenzoate groups and groups capable of Michael-type
  • haloalkyl any alkyl group of one to three carbon atoms substituted with bromine, iodine or chlorine.
  • IodoalkyI groups may be introduced into the structure of known chelators, including, but not limited to, diethylenetr ia inepentaacetic acid, ethylenediamine- tetraacetic acid, dimercaptosuccinic acid, 2,3- dimercaptopropanesulfonic acid, metallothioein and cryptates.
  • a compatible chelator (or compatible chelator-metal ion complex) is attached to an antibody directed against a target antigen, by (a) attachment to the carbohydrate moieties of the antibody or antibody fragment, or (b) attachment to sulfhydryl groups of the antibody molecule.
  • SUBSTITUTE SHEET * is used, the attachment must not significantly change the essential characteristics of the antibody or antibody fragment, such as immunospecificity and immunoreactivity. Additional considerations include simplicity of reaction and stability of the antibody conjugate produced.
  • Glycoproteins are biologically important 0 macromolecules which share structural characteristics including carbohydrate residues covalently attached to a polypeptide backbone. Since antibodies are glycoproteins, compounds may be attached to the carbohydrate moiety of the molecule. Some of the carbohydrate moieties are located on
  • the Fc region of the immunoglobulin 15 the Fc region of the immunoglobulin and are required in order for Cl binding to occur.
  • the carbohydrate moiety of the Fc region of, an immunoglobulin may be utilized in the scheme described herein.
  • the Fab or Fab' fragments of any immunoglobulins which contain carbohydrate moieties may be utilized in the scheme described herein.
  • the carbohydrate side 25 chains of antibodies or Fab or Fab 1 fragments may be selectively oxidized to generate aldehydes.
  • the resulting aldehydes may then be reacted with amine groups (e.g., ammonia derivatives such as a primary amine, hydroxylamine , hydrazine, hydrazide, phenylhydrazine , semicarbazide or 30 thiosemicarbazide) to form a Schiff base or reduced Schiff base (e.g., imine, enamine, oxime, hydra zone, phenylhydrazone , semicarbazone or thiosemicarbazone , or reduced forms thereof) .
  • amine groups e.g., ammonia derivatives such as a primary amine, hydroxylamine , hydrazine, hydrazide, phenylhydrazine , semicarbazide or 30 thiosemicarbazide
  • the carbohydrate moiety of the antibody may be modified by enzymatic techniques so as to enable attachment to or reaction with other chemical groups.
  • an enzyme is galactose oxidase which oxidizes galactose in the presence of oxygen to form an aldehyde.
  • Oxidation of the carbohydrate portion or moiety of antibody molecules leads to formation of aldehyde groups.
  • a variety of oxidizing agents can be used, such as periodic acid, paraperiodic acid, sodium me taper iodate and potassum metaperiodate .
  • oxygen acids and salts thereof are preferred since secondary or undesirable side reactions are less frequent.
  • Oxidation of antibodies with these oxidizing agents can be carried out by known methods.
  • the antibody is used generally in the form of an aqueous solution, the concentration being generally less than 100 mg/ml , preferably 1 to 20 mg/ml .
  • an oxygen acid or a salt thereof is used as the oxidizing agent, it is used generally in the form of an aqueous solution, and the concentration is generally 0.001 to lOmM and preferably 1.0 to lOmM.
  • the amount of the oxygen acid or salt thereof depends on the kind of antibody, but generally it is used in excess, for example, twice to ten times as much as the amount of the oxidizable carbohydrate. The optimal amount, however, can be determined by routine experimentation.
  • the optional ranges include a pH of from about 4 to 8 , a temperature of from 0 to 37°C, and a reaction period of from about 15 minutes to 12 hours.
  • Oxidation of the carbohydrate portion of antibody molecules may also be done with the enzyme, galactose oxidase (Cooper, et al . , 1959, J. Biol. Che . 234: 445-448) .
  • the antibody is used in aqueous solution, the concentration being generally 0.5 to 20 mg/ml.
  • the enzyme generally is used at about 5 to 100 units per ml of solution, at a pH ranging from about 5.5 to about 8.0. The influence of pH, substrate concentration, buffers and buffer concentrations on enzyme reaction are reported in Cooper, et al., supra .
  • the antibody-chelator conjugates (or antibody-metal ion complexes when pre-chelated metal ion is attached to the chelator prior to reaction of the chelator with antibody) of the invention may be produced by reacting the oxidized antibody with a compatible chelator having an available amine group selected from the group consisting of primary amine, hydrazine, hydrazide, hydroxyl amine , phenylhydrazine , semicarbazide and thiosemicarbazide groups.
  • the immediately resulting products contain a carbon-nitrogen
  • a solution of the oxidized antibody at a concentration of from about 0.5 to 20 mg/ml is mixed with the compatible chelator (molar ratios of reactive chelator group to antibody aldehyde ranging from about 1 to about 10,000) and the solution incubated for from about 1 to 18 hours. Suitable temperatures are from 0 to 37 °C and pH may be from about 6 to 8.
  • the antibody-chelator conjugates (or antibody-metal ion complexes) have been formed between the antibody and a compatible chelator as described in Section 5.3.1.3, they can optionally be stabilized with a suitable reducing agent, such as sodium cyanoborohydride or sodium borohydride :
  • Antibody-CH N-R
  • Antibody-CH -NH-R agent Reducing agent is generally added to a molar excess of from about 10 to 100 fold molar excess over available aldehyde
  • Free sulfhydryl groups can be generated from the disulfide bonds of the immunoglobulin molecule. This is accomplished by mild reduction of the antibody molecule.
  • the disulfide bonds of IgG which are generally most susceptible to reduction are those that link the two heavy chains.
  • the disulfide bonds located near the antigen binding region of the antibody molecule remain relatively unaffected. Such reduction results in the loss of ability to fix complement but does not interfere with antibody-antigen binding ability (Karush, et al . , 1979, Biochem. 1_8_: 2226-2232).
  • -.reactive groups include, but are not limited to, reactive haloalkyl groups (including, for example, haloacetyl groups)., p- mercuribenzoate groups and groups capable of Michael-type addition reactions (including, for example, maleimides and groups of the type described in Mitra and Lawton, 1979, J. A er , Chem. Soc . 101 : 3097-3110).
  • haloalkyl is meant any alkyl group of one to three carbon atoms substituted with bromine, iodine or chlorine.
  • Antibody-chelator conjugates (or antibody-metal ion complexes when pre-chelated metal ion is attached to the chelator prior to reaction of the chelator with antibody) which are produced by attaching a compatible chelator to a free sulfhydryl group of a reduced immunoglobulin or reduced antibody fragment do not activate complement.
  • complexes of the conjugates may advantageously be used in in vivo imaging systems where release of the metal ion is not desirable.
  • Chelator-metal ion complexes may be prepared by attaching the metal ion directly to the chelator. Conventional methods of attaching metal ions to chelators may be utilized to accomplish binding to chelator. For example,
  • Indium ( In) may be attached to a water-soluble chelator compound by incubation of In-C,., in sodium acetate with the chelator, e.g., for 1 hour at 37 °C.
  • the metal ion may be attached to the chelator either before or after the chelator is attached to the antibody molecule. Whatever method of attachment is utilized, it may be significant for many applications that any metal ion not specifically attached to the chelator compound be removed before the complexes are used, particularly JLr vivo. According to the preferred embodiment, the non-specif ically attached metal ⁇ on is removed using a high performance liquid molecular sieve chroma tog raphy system.
  • the antibody molecule or antibody fragment may be attached to a compatible chelator according to methods described herein, and the antibody- chelator conjugate intermediate may be purified, if necessary, and stored frozen at -20°C until needed. The metal ion 'may then be attached to the antibody-chelator conjugate
  • Indium may be attached by incubation of 111 " .InC, ⁇ with the antibody-chelator conjugate in acetate buffer, pH 6.0, at 37°C for 1 hour.
  • acetate buffer, pH 6.0 pH 6.0
  • Such method of attachment advantageously allows purification of the antibody-chelator intermediate without exposing the preparer to excessive handling of radioactive materials, and without necessity of lengthy storage of radioisotopes in the work areas .
  • the antibody-metal ion complexes of the invention are useful in a variety of therapeutic and _in vitro and in vivo diagnostic applications.
  • cellular disorder is meant to include all neoplasms, including cancers, adenomas, and hyperplasias; certain immunological disorders, including autoimmune diseases, graf t-versus-host diseases (e.g., after bone marrow transplantation) , immune suppressive diseases, e.g., after kidney or bone marrow transplantation.
  • Treatment of such cellular disorders involving, for example, bone marrow transplantation may include purging (by killing) undesired cells, e.g., malignant cells or mature T lymphocytes.
  • Therapeutic applications center generally on treatment of various cellular disorders, including those c broadly described above, by administering an effective amount of the antibody-metal ion complexes of the invention.
  • the properties of the antibody in being immunospecific for and immunoreactive with a particular antigen render it ideally suited for delivering metal ions to ' specific cells, tissues, organs or any other site having that particular antigen.
  • the antibody or antibody fragment of the antibody-metal ion complex functions to deliver the complex to the target site, at which site the metal ion, which is chosen for its cell 5 killing properties, is able to destroy nearby cells.
  • Suitable beta emitting ions for therapeutic uses include: 46 Scandium,
  • Suitable alpha emitting metal ions include those with a relatively short half-life of about four days or less, including 0
  • In vivo administration may involve use of pharmaceutical compositions of antibody-metal ion complex in 5 any suitable carrier, including serum or physiological saline, with or without another protein, such as human serum albumin. Dosage of the complexes may readily be determined by one of ordinary skill, and may differ depending upon the nature of the cellular disorder and the metal ion used. 30
  • Route of administration may be parenteral , with intravenous administration generally preferred.
  • In vivo diagnostic applications involve imaging of specific tissues or cellular disorders by administration of a
  • metal ions suitable for i ⁇ vivo tissue imaging have been tested and utilized clinically.
  • imaging with radioisotopes the following characteristics are generally recognized as desirable and/or necessary: (a) low radiation dose to the patient; (b) high photon yield which 5 permits a nuclear medicine procedure to be performed in a short time period; (c) ability to be produced in sufficient quanitites; (d) acceptable cost; (e) simple preparation for administration; and ( f ) no requirement for subsequent isolation of the patient.
  • positron emission tomography An alternative method for imaging with radioisotopes involves positron emission tomography. Suitable positron emitting isotopes include 43 Scandium, 44 Scandium, 52 Iron,
  • SUBSTITUTE SHEET Tissue imaging may also utilize nonradioactive paramagnetic metal ions such as 54 Iron, 56 Iron, 57 Iron, cp 157 55
  • Tissues which one may image include any solid neoplasm, certain organs such as lymph nodes, parathyroids, spleen and kidney, sites of inflammation or infection (e.g., macrophages at such sites) , myocardial infarction or thromboses ( neoantigenic determinants on fibrin or platelets) , etc .
  • immunoradiometric assays involve labeled antibodies to detect unlabeled antigens.
  • Numerous variations of the immunoradiometric assay have been developed, including for example, the two-site IRA and the indirect IRA. These two methods are discussed generally below.
  • the objective of the two-site IRA is to use a
  • Sepharose Sepharose
  • a second labeled antibody is bound to another site on the antigen.
  • the second antigen can then be measured as a function of the amount of bound labeled antibody.
  • the antigen is bound to two different antibodies at two sites on the antigen.
  • SUBSTITUTE SHEET ethod purified antigen is adsorbed or coupled to a solid phase support.
  • the (first) antibody used for measuring antigen is indirectly labeled through the use of a labeled antibody to the immunoglobulin of the same species as that in which the first antibody is raised. This labeled anti- immunoglobulin antibody is then reacted with the first antibody and antigen.
  • the labeled antibody can also be used in the conventional or two site IRAs .
  • all of these IRA methods for testing for antigen have in common the following two steps: (a) mixing an antibody-metal ion complex with a sample suspected of containing the antigen, and (b) detecting the interaction, if any antigen is present, of said complex- with the antigen.
  • i _ vitro diagnostics all of the gamma and positron emitting metal ions, as well as the paramagnetic metal ions in Section 5.5.2, as well as 153 Gadolinium are suitable.
  • lanthanides may be employed, including Praseodymium, Neodymium, Samarium,
  • Thulium and Ytterbium Thulium and Ytterbium.
  • SUBSTITUTE SHEET which according to the instant inventions are useful for in vivo imaging systems, for _ n vivo therapy and for j j vitro detection systems.
  • compatible chelators are utilized to attach a metal ion, such as a radioisotope, to an antibody or antibody fragment.
  • a metal ion such as a radioisotope
  • DTPA mixed anhydride The mixed anhydride of diethylenetriaminepentaacetic acid (DTPA mixed anhydride) was prepared according to the method described by Krejcarek and Tucker, 1977, Biochem. Biophys. Res. Commun. 77: 581-585. Briefly, 2.54 mmole DTPA and 12.7 mmole tr iethylamine were dissolved in 20 ml of water and the solution dried under vacuum- to yield the DTPA salt. The salt was then dissolved in 20 ml of a solvent system consisting of acetonitrile-dimethylformamide (CH..CN-DMF) in a ratio of 65:35, and cooled to -5°C in an ice-rock salt bath.
  • CH..CN-DMF acetonitrile-dimethylformamide
  • P-Am ino an il ine-d iethylenetri amine pen taace tic acid anhydride was prepared as follows. Briefly, 25.4 mmole p-phenylenediamine in 50 ml of a solvent system containing CH 3 CN-DMF (65:35) was added to 2.0 g of fresh DTPA mixed anhydride prepared as described in Section 6.1.1. The solution was maintained with stirring at -5°C for 2 hours under nitrogen, and then at room temperature (approximately 24 °C) overnight. At the end of this time period, the initially homogeneous solution had become a heterogeneous mixture.
  • This heterogeneous reaction mixture was then evaporated to dryness under vacuum, and the resulting residue dissolved in a small aliquot 50 ml of distilled water.
  • the pH of the reaction mixture was adjusted to 11.0 using sodium hydroxide (NaOH) solution (6M) . Unreacted p-phenylenediamine was removed by extraction with methylene chloride. The aqueous phase was collected, concentrated under vacuum and lyophilized to yield a pale (white) powder of crude ADTPA.
  • the crude ADTPA was purified by elution through a packed silica gel column using ethanol- aqueous ammonia (4:1), followed by distilled water.
  • KDTPA ⁇ -N-Diethylenetriaminepentaacetic acid L-lysine
  • SUBSTITUTE SHEET (65:35) was added. The reaction mixture was maintained with stirring under nitrogen for 2 hours at 0°C, and then overnight at room temperature. The solution was evaporated to dryness under vacuum and the resulting residue dissolved in 50 ml distilled water. The unreacted lysine ester was removed by adjusting the pH to 11.0 with NaOH solution and extracting with methylene chloride. The aqueous solution was then concentrated under vacuum and the pH adjusted to neutrality with a small aliquot of hydrochloric acid (HC1) . The carbobenzoxy protecting group was then removed using palladium/ activated charcoal (5% palladium) under 30 lb-inch 2 hydrogen for 4 hours in a Parr Hydrogenator . The resulting
  • the hydrazide derivative of DTPA was prepared as follows: 6.3 mmole of anhydrous hydrazine was dissolved in 2 ml dimethylformamide (DMF) and reacted with 2.54 mmole DTPA mixed anhydride in 20 ml CH 3 CN-DMF (65:35) . The heterogeneous mixture was maintained with stirring at 4°C for 1 hour, and then at room temperature for 1 hour. The reaction .mixture was evaporated to dryness under vacuum, and the residue dissolved in ethanol-aqueous ammonia (4:1) . The product was then purified by elution through silica gel with ethanol-aqueous ammonia, followed by distilled water. Product-containing fractions were collected, and the pH adjusted to 3.0 using a small aliquot of hydrochloric acid.
  • the glycyl-tyrosyl-lysine-diethylenetria ine- pentaacetic acid anhydride (GYK-DTPA) was prepared as follows.
  • the initial peptide reactant N-FMOC-glycyl-(o- benzyl-tyrosyl-( €-N-carbobenzoxy) ) lysine was ' prepared according to standard solid phase synthetic methods described by Baranz and Merrifield, JEN The Peptides, Vol. 2, Gross and Meienhoffer (ed.) , Acad . Press, New York, pp. 3-385.
  • the derivatized peptide was cleaved from the resin and partially deblocked by bubbling hydrogen bromide (HBr) gas through a suspension of the reaction mixture in trifluoroacetic acid containing methoxybenzene (a fifty-fold molar excess over tyrosine) for 60 minutes at room temperature.
  • HBr hydrogen bromide
  • the resin was removed by filtration and the filtrate evaporated to dryness. The residue was dissolved in a minimum amount of methanol, and the product precipitated with ether and used without further purification.
  • SUBSTITUTE SHEET solvent was evaporated to dryness and the residue taken up in distilled water.
  • the crude product was purified by extraction with ethyl acetate, and the resulting aqueous solution of GYK-DTPA was lyophilized to dryness.
  • Polyethylene imine-diethylenetriaminepentaacetic acid was prepared as follows: 2.54 mmole DTPA was dissolved in 100 ml H ⁇ O, and the pH was adjusted to 5.0 using 6M NaOH solution. Then 12.7 mmole l-ethyl-3-(3- dimethyla inopropyl) carbodiimide (Sigma Chemical Co., St. Louis, MO) and 144 ⁇ mole polyethylene imine (Polysciences , Inc. Warring ton, PA) were added. The pH was readjusted to 5.0 using aqueous HC1 , and the solution was maintained with stirring at room temperature overnight. The PEI-DTPA formed was separated from the reaction mixture either by reverse phase chroma tography or by gel filtration.
  • P-Nitrobenzylamine HC1 (174 mg , 0.92 mmole) was treated with 3 ml of 0.3N NaOH to form yellow oil and the oil was extracted with CH ⁇ Cl.-. The organic layer was separated and dried _i ⁇ vacuo to obtain the p-nitrobenzylamine free base .
  • DTPA anhydride (CDTPA) was synthesized according to the procedure of Hnatowich, et al . (1982, Int. J. Appl . Radiat. Isot. 33: 327-332) .
  • DTPA anhydride (CDTPA) was synthesized according to the procedure of Hnatowich, et al . (1982, Int. J. Appl . Radiat. Isot. 3_3_: 327-332) . 0
  • the specific monoclonal antibody utilized in the following experiments for injection into experimental animals was a rat IgG antibody specific .for a Class I major histocompatability complex (MHC) antigen of Brown Norway (BN) rats.
  • MHC major histocompatability complex
  • BN rats Brown Norway
  • This antibody, designated CYT-015 was prepared by 0 fusion of spleen cells from Lewis rats previously immunized by injection with cells from BN rats, with myeloma cell line SP2/0 AG14 according to the method described by McKearn, et al . (1979, Immunol. Rev. 47_: 91-115).
  • CYT- 015-containing ascites were generated by intra eritoneal 5 injection of cells into pristane-primed nude mice.
  • IgG monoclonal antibody was purified from this ascites by specific elution on a Protein-A-Sepharose column (Pharmacia Fine Chemicals, Piscataway, NJ) .
  • a mouse monoclonal IgG antibody specific for a monomorphic determinant of a human Class I major histocompatibility complex was used as a carrier antibody for
  • Radiolabeled antibody-metal ion complexes were prepared according to one method of the instant invention by first forming antibody-chelator conjugates.
  • the carbohydrate moiety of the antibody (CYT-012 or CYT-015) was oxidized by reacting approximately 1 mg/ml glycoprotein in phosphate buffered saline (PBS) with 10 mM sodium metaperiodate (NalO.) (pH 6.0) for 1 hour on ice in the dark.
  • PBS phosphate buffered saline
  • NaalO. sodium metaperiodate
  • the sample was then passed through a Sephadex ® G-25 column (Pharmacia Fine Chemicals, Piscataway, NJ) and the protein fractions pooled.
  • the oxidized antibody was then attached to a compatible chelator and either used immediately or stored frozen at -20°C.
  • GYK-DTPA glycyl-tyrosyl-lysine-diethylene- triaminepentaacetic acid
  • oxidized antibody was coupled either to p-aminoaniline-diethylenetriaminepentaacetic acid (ADTPA) or polyethylene imine-diethyenetr iaminepentaacetic
  • Radioactive metal ion was then attached to the antibody-chelator conjugates in order to form radioactive antibody-metal ion complexes useful for imaging systems.
  • FIG. 1 illustrates an HPLC chromatogram obtained during purification and isolation of
  • Ficoll 70 was obtained from Pharmacia Fine Chemicals, Inc. (Piscataway, New Jersey). The carboxymethyl derivative of Ficoll 70 (CM-Ficoll) was prepared exactly as described by Inman (1975, J. Immunol. 114: 704). Two grams of CM-Ficoll were dissolved in 100 ml of water and 10 g of adipic dihydrazide were added slowly. The pH was adjusted to 4.7 by the dropwise addition of IN HC1 and then 1.25 g of 1- ethyl-3-(3-dimethyl-aminopropyl) cafbodiimide were added and the pH was adjusted to 4.7 with IN HC1. The reaction mixture was then stirred for 20 hours at 23-25°C.
  • CM-Ficoll carboxymethyl derivative of Ficoll 70
  • the crude reaction product was purified by gel filtration chromatography on a 4.5 x 55 cm column of Sephadex ® G-25.
  • the column was eluted with phosphate buffered saline (PBS, 0.01 M sodium phosphate, 0.15 M sodium chloride, pH 7.4) and the void volume fractions were saved.
  • PBS phosphate buffered saline
  • the hydrazide-Ficoll was then dialyzed against water and lyophilized.
  • the final product contained about 150 moles of hydra z id e/mole of Ficoll.
  • N-succinimdyl-3-(2- pyridyl-dithio)-propionate (Pharmacia Fine Chemicals, Inc., Piscataway, NJ) dissolved in 1.0 ml of tetrahydrofuran were added to the hydrazide-DTPA solution and the reaction mixture was stirred for 16 hours at 23-25 °C. The tetrahydrofuran was removed by bubbling N « through the solution and the resultant
  • mice anti-N. gonorrhoeae monoclonal IgG was diluted into 0.25 ml of PBS, pH 7.4. The IgG solution was then reduced with 5 mM dithiothreitol for 30 ° minutes at 23-25 °C. The reduced antibody was then passed over a 1x19 cm Sephadex ® G-50 column and eluted with 0.1 M tris(hydroxymethyl)-aminomethane buffer, pH 8.0, containing ImM ethylenediaminetetracetic acid (Tris/EDTA) .
  • Tris/EDTA ImM ethylenediaminetetracetic acid
  • IgG antibody (Cooper Biomedical, Scientific Division,
  • Sepharose 4B (Sigma Chemical Co., St. Louis, MO) .
  • the bound 0 complex was eluted with 0.1 M glycine buffer, pH 2.0 and desalted into Tris/EDTA buffer using Sephadex ® G-25.
  • the antibody was diluted with PBS, pH 7.4 to a final volume of 0.577 ml. 0.702 ml of 5
  • the (4-iodoacetyl)-aminobenzoichydrazide-Ficoll-IgG conjugate was purified by immuno ad sorption with a resin composed of sheep anti-mouse IgG (Cooper Biomedical,
  • SUBSTITUTE SHEET ml of tetrahydrofuran Six 0.5 ml aliquots of the SMCC solution were added to 20 ml of a 5 mg/ml solution of hydrazide-Ficoll (prepared as described in Section 7.1), dissolved in 0.1 M sodium phosphate buffer, pH 6.8, at 30 minute intervals. Two milliliters of tetrahydrofuran were added to the reaction mixture before the second and third additions of SMCC solution. The organic solvents were then removed by bubbling N 2 through the reaction mixture and the resultant cloudy solution was clarified by centrifugation. The clear supernatant was lyophilized and the excess SMCC was extracted with tetrahydrofuran.
  • the organic solvent was then removed by evaporation under reduced pressure.
  • the final product contained about 15 moles of maleimide/mole of Ficoll.
  • This compound can be used in the same way as the (4- iodoacetyD-aminobenzoichydrazide-Ficoll prepared by the method of Section 7.2.
  • The_ following examples illustrate methods for in vivo imaging to locate specific tissue or organ components utilizing the radiolabeled antibody-metal ion complexes prepared according to the instant invention.
  • radiolabeled antibody-metal ion complexes were utilized to specifically locate tumorous tissues in experimental animals using a f) u radioimagmg system.
  • mice were injected subcutaneously in the left g hindquarter with 1 X 10 BN rat lymphoma cells. Seven days
  • the animals received a retro-orbital sinus or intravenous injection of 10 ⁇ g Indium-labeled anti-BN major histocompatability complex (MHC) antibody-metal ion ccoommpplleexx,, li:L IInn--CCYYTT--001155--AADDTTPPAA.. ]No animal received more than a single injection with complex.
  • MHC Indium-labeled anti-BN major histocompatability complex
  • mice Two groups of animals served as controls.
  • One control group of non-tumor-bearing nude mice were injected either in the retro-orbital sinus or intravenously with 10 ⁇ g
  • Radioimaging was accomplished as follows: at 24, 48 or 72. hours post-injection with radiolabeled antibody-metal ion complex, animals were placed directly upon the inverted face of a gamma camera (General Electric Maxi Camera 37 interfaced with an ADEC Clinical Data System) . Ten thousand - 25,000 counts were routinely accumulated on exposed X-ray film without correction for background radiation.
  • FIG. 2 illustrates in vivo images of radioactivity accumulated within BN tumor-bearing nude mice injected with
  • FIG. 2 clearly demonstrates that the complexes of the invention are specifically localized at the intended target with extremely low levels of non-specific localization.
  • FIG. 3 illustrates in vivo images of radioactivity in BN tumor-bearing control mice described above. Images E and F were taken 24 and 72 hours after injection,
  • FIG. 4. presents in vivo images of non-tumor-bearing nude mice injected with In-CYT-012-ADTPA. Images G and H were taken 24 and 72 hours after injection, respectively. As shown in FIG. 4, there was also no detectable localization of radioactivity in non-tumor-bearing nude mice injected with radiolabeled antibody-metal ion complex, In-CYT-015-ADTPA, specific for BN tumor antigen.
  • radiolabeled antibody-metal ion complexes were utilized to specifically locate transplanted renal tissues by in vivo radioimaging.
  • mice bearing a transplanted functioning kidney or renal allograft were prepared as follows: the left kidney of a Lewis rat was surgically removed and a kidney from a BN x Lewis Fl rat was implanted in its place. The recipients' immune response against the foreign kidney transplant was chemically suppressed (see Stuart, et al., 1980, Immunol. Rev. 4_9_: 127-165). Thus, the recipient rats possessed two functioning kidneys, only one of which carried the BN MHC antigen specifically recognized by CYT-015 antibody.
  • the recipient rats were intravenously ' injected with 10 ⁇ g In-
  • CYT-015-ADTPA and imaged as described in Section 8.1.
  • images A and B were taken approximately 1 hour and 20 minutes after intravenous injection.
  • image A the radionuclide localized within the transplanted kidney within approximately 1 hour and 20 minutes after intravenous injection of the labeled antibody-metal ion complex. Minimal accumulation of radioactivity was detected in the animal's kidney, lungs, spleen or liver.
  • FIG. 5, image B illustrates the image obtained when the same recipient animal was injected with a
  • DTPA chelate (Mallinkrodt, St. Louis, MO). Clearly, images obtained with mTechnetium-DTPA chelate are inferior to those obtained with the antibody-metal ion complexes of the invention.

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DK195186A (da) 1986-04-29
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JPS62500119A (ja) 1987-01-16
JPH0651720B2 (ja) 1994-07-06
GR852111B (forum.php) 1986-01-02
AU588832B2 (en) 1989-09-28
CA1260827A (en) 1989-09-26
EP0173629A1 (en) 1986-03-05
JPH06234800A (ja) 1994-08-23
DE3586188T2 (de) 1992-12-03
JPH0733399B2 (ja) 1995-04-12
DE3586188D1 (de) 1992-07-16
EP0173629B1 (en) 1992-06-10
AU4770185A (en) 1986-03-24

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