WO1984002777A1 - Method for the volumetric differential of leukocytes - Google Patents

Method for the volumetric differential of leukocytes Download PDF

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Publication number
WO1984002777A1
WO1984002777A1 PCT/US1983/001976 US8301976W WO8402777A1 WO 1984002777 A1 WO1984002777 A1 WO 1984002777A1 US 8301976 W US8301976 W US 8301976W WO 8402777 A1 WO8402777 A1 WO 8402777A1
Authority
WO
WIPO (PCT)
Prior art keywords
lysing reagent
populations
lysing
leukocytes
diluent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1983/001976
Other languages
English (en)
French (fr)
Inventor
Stephen L Ledis
Ted Sena
James H Ii Carter
Harold R Crews
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Electronics Inc
Original Assignee
Coulter Electronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23806640&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1984002777(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Coulter Electronics Inc filed Critical Coulter Electronics Inc
Priority to EP84900362A priority Critical patent/EP0131020B2/en
Priority to DE19833390432 priority patent/DE3390432T1/de
Priority to JP84500436A priority patent/JPS60500097A/ja
Priority to AU24134/84A priority patent/AU570943B2/en
Publication of WO1984002777A1 publication Critical patent/WO1984002777A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
    • G01N15/131—Details
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]

Definitions

  • the resulting suspension is passed through sensing apertures in a leukocyte counting bath, wherein the leukocytes or white blood cells are counted and sized electronically.
  • a leukocyte counting bath wherein the leukocytes or white blood cells are counted and sized electronically.
  • the erythrocytes must be reduced rapidly to very small fragments, to avoid interference with leukocyte counting.
  • the leukocytes must not be destroyed, even though they will shrink in size to a greater or lesser degree.
  • a quaternary ammonium salt advantageously is employed as a stromatolysing agent, with the virtually instantaneous destruction of erythrocytes to a level avoiding interference with leukocyte sizing and counting.
  • lymphocytes and monocytes are more sensitive than the granulocytes to the lytic agents usually employed.
  • the granulocytes were less "shocked", i.e. subject to a lower or low gradient of lytic shock, and thereby not reduced in size to the extent caused by prior reagent systems and methods. This is accomplished by treating the diluted blood sample with the lyse reagent less rapidly, and when the lyse reagent is in lower concentration than is routinely the case.
  • germicidal means said diluent having a predetermined pH and osmolality
  • a lysing agent which is an aqueous solution of quaternary ammonium salts having surface active properties.
  • a suitable chromagen for hemoglobin determination there also can be provided an alkali metal cyanide.
  • alkali metal cyanide there also can be provided.
  • Other chromagen-forming agents also can be employed.
  • R 1 is a long chain alkyl radical having 10 to 18 carbon atoms
  • R 2 , R 3 , R 4 are short chain alkyl radicals having 1 to 6 carbon atoms
  • X- is a salt forming radical or anion, such as C1-, Br- , PO 4 -3 and CH 3 SO 4 -.
  • the long chain alkyl has 12 to 16 carbon atoms
  • the short chains are methyl or ethyl
  • X- is chloride or bromide.
  • the preferred lysing agent employs a combination of dodecyltrimethylammonium chloride, with tetradecyltrimethylammonium bromide and potassium cyanide.
  • the ranges of pH and osmolality of the diluent used for this invention can be broader than shown in the Prior Art Example, for example: pH 7.0 ⁇ 0.4; osmolality 320 ⁇ 50 mOs/Kg.
  • the new result of producing a three-population histogram instead of the known two-population histogram is achieved by diluting the commercial lysing agent, sold under the trademark Lyse S ® plus to about one-half its concentration and then using about twice the volume of the lysing agent.
  • concentrations of active ingredients in the lysing agent formulations can be altered if the volume of lysing reagent to be used is modified correspondingly, as long as the ratios of the lysing components are maintained within certain limits.
  • the reagent system and method of this invention are designed to produce a triphasic size distribution of leukocytes so the pulse height analyzer in the Coulter Counter ® Model S Plus system can identify and count lymphocytes, mononuclear (monocyte) cells and granulocytes.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Ecology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • External Artificial Organs (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
PCT/US1983/001976 1983-01-03 1983-12-15 Method for the volumetric differential of leukocytes Ceased WO1984002777A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP84900362A EP0131020B2 (en) 1983-01-03 1983-12-15 Method for the volumetric differentiation of leukocytes
DE19833390432 DE3390432T1 (de) 1983-01-03 1983-12-15 Verfahren zur volumetrischen Differenzierung von Leukozyten
JP84500436A JPS60500097A (ja) 1983-01-03 1983-12-15 白血球の母集団の容量を区別する方法
AU24134/84A AU570943B2 (en) 1983-01-03 1983-12-15 Method for the volumetric differential of leukocytes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/454,926 US4485175A (en) 1983-01-03 1983-01-03 Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes

Publications (1)

Publication Number Publication Date
WO1984002777A1 true WO1984002777A1 (en) 1984-07-19

Family

ID=23806640

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1983/001976 Ceased WO1984002777A1 (en) 1983-01-03 1983-12-15 Method for the volumetric differential of leukocytes

Country Status (7)

Country Link
US (1) US4485175A (https=)
EP (1) EP0131020B2 (https=)
JP (1) JPS60500097A (https=)
AU (1) AU570943B2 (https=)
DE (1) DE3390432T1 (https=)
ES (1) ES8407590A1 (https=)
WO (1) WO1984002777A1 (https=)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0144339A4 (en) * 1983-03-25 1988-07-14 Coulter Electronics METHOD AND STROMATOLIZING AGENT FOR THE VOLUMETRIC DETERMINATION OF LEUKOCYTE POPULATIONS.
EP0161770A3 (en) * 1984-05-14 1988-11-02 Becton, Dickinson And Company Lysing agent for analysis of peripheral blood cells
EP0177137A3 (en) * 1984-09-24 1988-11-02 Technicon Instruments Corporation White blood cell differentiation composition and method
FR2621695A1 (fr) * 1987-10-12 1989-04-14 Abx Sa Reactif de lyse pour la determination des populations de leucocytes par differenciation volumetrique
EP0375201A1 (en) * 1988-12-07 1990-06-27 BioChem Immunosystems (U.S.), Inc. A method of separating the three major types of blood cells from a white blood cell histogram
EP0381669A4 (en) * 1987-03-13 1991-01-02 Coulter Electronics Inc. Automated analyzer and method for screening cells or formed bodies for enumeration of populations expressing selected characteristics
EP0349591A4 (en) * 1987-03-13 1991-01-16 Coulter Electronics Inc. Method and apparatus for rapid mixing of small volumes for enhancing biological reactions
EP0424871A1 (en) * 1989-10-23 1991-05-02 Toa Medical Electronics Co., Ltd. Leukocytes and hemiglobin in blood
EP0444241A1 (en) * 1990-03-01 1991-09-04 Toa Medical Electronics Co., Ltd. Reagent for measurement of leukocytes and hemoglobin in blood
WO1995017252A1 (en) * 1993-12-23 1995-06-29 Abbott Laboratories Method of using disposable reagent pack
US5501982A (en) * 1993-12-20 1996-03-26 Abbott Laboratories Method of using a disposable reagent pack
WO2002014861A1 (en) * 2000-08-17 2002-02-21 Mallinckrodt Baker B.V. Method and reagent for the analysis of blood samples, in particular for veterinary applications

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US4962038A (en) * 1980-06-16 1990-10-09 Coulter Electronics, Inc. Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4529705A (en) * 1983-06-06 1985-07-16 Coulter Electronics, Inc. Reagent for combined diluting and lysing whole blood
US4637986A (en) * 1983-08-22 1987-01-20 Ortho Diagnostic Systems, Inc. Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part WBC count
US4614722A (en) * 1983-11-01 1986-09-30 Pasula Mark J Method and apparatus for measuring the degree of reaction between antigens and leukocyte cellular antibodies
US4575490A (en) * 1984-02-29 1986-03-11 Technicon Instruments Corporation One step method for sphering and fixing whole blood erythrocytes
US4704364A (en) * 1984-05-18 1987-11-03 Coulter Electronics, Inc. Hematology control compositions for three populations of leukocytes; and methods for their preparation and use in whole blood control systems
US4751179A (en) * 1984-05-31 1988-06-14 Coulter Electronics, Inc. Method and reagents for differential determination of four populations of leukocytes in blood
DE3521544A1 (de) * 1985-06-15 1986-12-18 Merck Patent Gmbh, 6100 Darmstadt Loesungsmittel fuer die wasserbestimmung nach karl-fischer
US4745071A (en) * 1985-09-05 1988-05-17 Sequoia-Turner Corporation Method for the volumetric differentiation of blood cells types
US5175109A (en) * 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
US5155044A (en) * 1987-03-13 1992-10-13 Coulter Electronics, Inc. Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples
IL85532A (en) * 1987-03-13 1992-03-29 Coulter Electronics Method and lytic reagent system for isolation,identification and/or analysis of leukocytes from whole blood samples
US4882284A (en) * 1987-04-13 1989-11-21 Ortho Pharmaceutical Corporation Method for quantitating and differentiating white blood cells
US5045474A (en) * 1987-05-28 1991-09-03 Sequoia-Turner Corporation (Unipath) Semi-automatic process for white cell differential count
US5389549A (en) * 1987-05-29 1995-02-14 Toa Medical Electronics Co., Ltd. Method for classifying leukocytes and a reagent used therefor
AU625334B2 (en) * 1988-07-14 1992-07-09 Board Of Regents, The University Of Texas System Intramedullary catheter
US5008202A (en) * 1988-11-29 1991-04-16 Sequoia Turner Corporation Blood diluent for red blood cell analysis
CA2016699C (en) * 1989-05-15 2003-11-18 Paul N. Marshall Lytic agents and uses thereof
US5284940A (en) * 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
US5654179A (en) * 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
US5620852A (en) * 1990-11-14 1997-04-15 Hri Research, Inc. Nucleic acid preparation methods
JP2927979B2 (ja) * 1991-02-22 1999-07-28 シスメックス株式会社 フローサイトメトリーによる赤芽球の分類方法
US5316951A (en) * 1991-06-07 1994-05-31 Carver Jr Edward L Method for the improved determination of white blood cell subpopulations
US5316725A (en) * 1991-06-07 1994-05-31 Edward Lawrence Carver, Jr. Reagent system for the improved determination of white blood cell subpopulations
US5262329A (en) * 1991-06-13 1993-11-16 Carver Jr Edward L Method for improved multiple species blood analysis
EP0628166B1 (en) * 1992-02-24 2000-05-31 Coulter Corporation Suspension media for hematological composition and method for its use
US6509192B1 (en) 1992-02-24 2003-01-21 Coulter International Corp. Quality control method
US6362003B1 (en) 1992-02-24 2002-03-26 Coulter Corporation Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof
ATE195810T1 (de) * 1992-02-24 2000-09-15 Coulter Corp Hämatologisches kontrollprodukt mit leukozytenanalogen; und methoden zu ihrer herstellung und anwendung
JPH08501208A (ja) * 1992-06-12 1996-02-13 ジェン−プローブ・インコーポレイテッド 血液から核酸の製造
DE69327775T2 (de) * 1992-11-19 2000-06-21 Sysmex Corp., Kobe Verfahren zur Vorbehandlung für Blutanalyse
JP3467310B2 (ja) * 1994-04-21 2003-11-17 シスメックス株式会社 白血球分析用試薬及び白血球の分類方法
WO1996002841A1 (en) 1994-07-14 1996-02-01 Abbott Laboratories Methods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood
US5834315A (en) * 1994-12-23 1998-11-10 Coulter Corporation Cyanide-free reagent and method for hemoglobin determination and leukocyte differentitation
US5843608A (en) * 1995-06-08 1998-12-01 Coulter International Corp. Reagent and method for differential determination of leukocytes in blood
US5882933A (en) * 1995-06-08 1999-03-16 Coulter International Corp. Method for determination of leukocytes and hemoglobin concentration in blood
US5686308A (en) * 1995-06-08 1997-11-11 Coulter Corporation Reagent and method for differential determination of leukocytes in blood
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US5763280A (en) * 1997-01-21 1998-06-09 Coulter International Corp. Cyanide-free lytic reagent composition and method for hemoglobin and cell analysis
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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0144339A4 (en) * 1983-03-25 1988-07-14 Coulter Electronics METHOD AND STROMATOLIZING AGENT FOR THE VOLUMETRIC DETERMINATION OF LEUKOCYTE POPULATIONS.
EP0161770A3 (en) * 1984-05-14 1988-11-02 Becton, Dickinson And Company Lysing agent for analysis of peripheral blood cells
EP0177137A3 (en) * 1984-09-24 1988-11-02 Technicon Instruments Corporation White blood cell differentiation composition and method
EP0381669A4 (en) * 1987-03-13 1991-01-02 Coulter Electronics Inc. Automated analyzer and method for screening cells or formed bodies for enumeration of populations expressing selected characteristics
EP0349591A4 (en) * 1987-03-13 1991-01-16 Coulter Electronics Inc. Method and apparatus for rapid mixing of small volumes for enhancing biological reactions
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ES528523A0 (es) 1984-10-01
DE3390432T1 (de) 1985-04-04
DE3390432C2 (https=) 1988-09-01
US4485175A (en) 1984-11-27
AU2413484A (en) 1984-08-02
AU570943B2 (en) 1988-03-31
EP0131020A1 (en) 1985-01-16
ES8407590A1 (es) 1984-10-01
EP0131020B2 (en) 1995-01-04
EP0131020B1 (en) 1989-08-02
EP0131020A4 (en) 1985-07-01
JPS60500097A (ja) 1985-01-24

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