WO1983004309A1 - Procede de preparation d'une serie d'echantillons pour l'analyse de melanges et installation pour l'execution automatique du procede - Google Patents

Procede de preparation d'une serie d'echantillons pour l'analyse de melanges et installation pour l'execution automatique du procede Download PDF

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Publication number
WO1983004309A1
WO1983004309A1 PCT/CH1983/000038 CH8300038W WO8304309A1 WO 1983004309 A1 WO1983004309 A1 WO 1983004309A1 CH 8300038 W CH8300038 W CH 8300038W WO 8304309 A1 WO8304309 A1 WO 8304309A1
Authority
WO
WIPO (PCT)
Prior art keywords
filter
sample
filter strip
series
strip
Prior art date
Application number
PCT/CH1983/000038
Other languages
German (de)
English (en)
French (fr)
Inventor
Fritz Ackermann
Anton Bill
Original Assignee
Ismatec Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ismatec Sa filed Critical Ismatec Sa
Priority to DE8383900896T priority Critical patent/DE3371119D1/de
Publication of WO1983004309A1 publication Critical patent/WO1983004309A1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4077Concentrating samples by other techniques involving separation of suspended solids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873Cutting or cleaving
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873Cutting or cleaving
    • G01N2001/288Filter punches
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/11Automated chemical analysis
    • Y10T436/110833Utilizing a moving indicator strip or tape
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/25Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/25Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575Volumetric liquid transfer

Definitions

  • the invention relates to a method for producing a series of samples for the analysis of batches, in which the batch in question is removed from a vessel with a liquid sample batch and placed in places on a step-by-step filter strip, and the filtrate that is no longer required is suctioned off from the rear of the filter strip and the filter residue is prepared for the analysis.
  • Such methods are e.g. used for the preparation of samples for immunological analyzes or for the analysis of suspended substances in liquids or gases.
  • a filter strip is used to produce the samples, which is attached to a carrier tape made of stable material and provided with windows in places.
  • the samples can be protected against contamination by covering on both sides and stored by rolling up the carrier tape.
  • Such a filter strip with carrier tape is suitable, but is only suitable for dry samples and, moreover, is very complex in terms of material and manufacture.
  • the batch is pipetted onto the filter strip for filtering. Without the use of special protective measures, there is a certain risk of contamination.
  • an additional feed pump is required to supply the batch to the pipette.
  • the feed path is lengthened and, as a result, and because of the use of slow-working peristaltic pumps, the duration of the batch transport is increased, which in certain cases can lead to incorrect changes in the sample batch.
  • a longer feed path also requires an increased effort
  • the object of the invention is to provide a method of the type mentioned at the outset, which makes it possible to produce samples for batch analyzes while avoiding the disadvantages mentioned and with simpler and less complex means and thereby achieve shorter throughput times and a reduction in the risk of contamination .
  • the feed pump is dispensed with in the feed path. This can be kept shorter, which enables a faster throughput and facilitates cleaning.
  • the complete sealing at the filter site offers protection against any material exchange with the environment. Parts of the filter containing
  • Filter strips are punched out each time the batch is deposited and transferred to sample vessels.
  • the strength of the filter strip is therefore less stringent than when the entire filter strip is stored with the associated mechanical stresses, which is why a carrier tape can be dispensed with.
  • a method which works with unreinforced filter strips, but the filter strips provided with coated filter points are cut by hand into parts with one filter point each and introduced into the sample vessels. Contamination and errors can hardly be avoided with this method, and handling is cumbersome and time-consuming.
  • the invention also relates to a device for the automatic implementation of the method, which is characterized by the features specified in claim 2.
  • An exemplary embodiment of the device according to the invention is shown schematically in the drawing. It can be a separately constructed filter station that can be used in an automatic analysis system for series analysis.
  • the prepared and optionally pretreated sample batches are located in open or closed vessels, which e.g. progressively moved in a chain and successively fed to the filter station shown. According to the drawing, such a sample preparation vessel 1 is located at the point intended for sampling.
  • a sampling device with a raised and lowered cannula 2 is used, which is connected to a lifting motor 3.
  • a pipette 4 is mechanically verbun ⁇ to, via which any additions and the "Rein Trentsflüs ⁇ supplied fluid.
  • the filter strip 5 for example made of glass fiber paper, is removed from a roller 6 and moved along a guide track 9 step by step by means of feed rollers 7, 8.
  • the filtering device 10 consists of a suction head 12 arranged below the guideway and a vertically movable bell 13 arranged above the guideway opposite the suction head.
  • the suction head 12 is at the level of the Guide track 9 is closed by a filter carrier 14, for example in the form of a sintered glass plate, and connected to a vacuum pump (not shown) via a controlled valve 15.
  • the bell 13 is connected to a lifting motor 16, which can press the bell against the suction head 12 on the filter strip lying in between, so that the filter point is sealed on both sides.
  • a sealing ring 17 can be provided at the lower edge of the bell.
  • a line 18 with a preferably height-adjustable mouth is inserted into the bell 13 and is directly connected to the cannula 2 of the removal device via a hose 19.
  • the punching device 11 has a punch 20 and a lifting motor 21 for its actuation. In this way, a disk of the filter strip is punched out at the punched opening 22 in the guideway 9.
  • a darning needle 23 is guided longitudinally in the punch 20 and connected to a lifting motor 24.
  • the stuffing needle 23 conveys the punched-out disk into a sample receptacle 25 underneath, which e.g. again in a chain of receptacles.
  • the reagent liquid is fed to the stuffing needle 23 via a hose 26.
  • the filtering station works in time with the feed devices (not shown) for the transport of the preparation vessels 1 and the receiving vessels 25.
  • a program control device can be provided which controls the activity of the lifting motors 3, 16, 21, 24, of the The drive of the feed rollers 7, 8 and the valve 15 controls and, for example, starts or stops the vessel of a chain of sample preparation vessels from the first and last to the removal device. This gives e.g. the following working method of the filter station:
  • the cannula 2 is lowered into the preparation vessel 1 and the bell 13 onto the stationary filter strip 5. Then the necessary addition is made, e.g. in the form of a buffer solution, via the pipette 4. Before the end of this addition, the valve 15 is opened, whereby the suction power of the vacuum pump comes into effect and the sample batch from the batch vessel 1 via the cannula 2 and the hose 19 to the filter point within the bell 13 transported. As a result of the pumpless connection between cannula 2 and bell 13, this transmission takes place within a few seconds, so that the duration of the influence of any addition on the sample can be kept within desired limits.
  • the vessel chains and the filter strip 5 are advanced by one step, so that the next batch vessel 1 comes into the removal position, the first sample collection vessel 25 into the acquisition position and the filter point covered with the filter residue comes under the punch 20 .
  • the reagent liquid for example a scintillation liquid in the case of radioimmunological samples, is fed through the hose 26 through the latter.
  • the end of the stuffing needle 23 is preferably expanded in a trumpet shape so that the filter disc is grasped as close as possible to the edge and cannot come into contact with the outside of the stuffing needle when it is inserted into the receptacle 25.
  • Reagent liquid also serves to clean the darning needle.
  • the supply of the various liquids via the pipette 4 and the hose 26 is preferably carried out by selectively connectable dispensers.
  • the lifting motors 3 and 16 can be replaced by a single lifting motor with a suitable arrangement of the removal device and the bell 13.
  • An overpressure spring is preferably provided for pressing the bell 13 onto the filter strip 5.
  • a filter disc can be punched out and the next sample can be filtered at the same time.

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
PCT/CH1983/000038 1982-06-05 1983-03-24 Procede de preparation d'une serie d'echantillons pour l'analyse de melanges et installation pour l'execution automatique du procede WO1983004309A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE8383900896T DE3371119D1 (en) 1982-06-05 1983-03-24 Device for the automatic preparation of a series of samples to analyze mixtures

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH348582A CH655392B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1982-06-05 1982-06-05
CH3485/82-7 1982-06-05

Publications (1)

Publication Number Publication Date
WO1983004309A1 true WO1983004309A1 (fr) 1983-12-08

Family

ID=4257002

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CH1983/000038 WO1983004309A1 (fr) 1982-06-05 1983-03-24 Procede de preparation d'une serie d'echantillons pour l'analyse de melanges et installation pour l'execution automatique du procede

Country Status (5)

Country Link
US (1) US4568520A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (1) EP0110890B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CH (1) CH655392B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DE (1) DE3371119D1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO1983004309A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0148290A1 (en) * 1983-12-14 1985-07-17 Försvarets Forskningsanstalt Method and device at the analysis of liquid samples
EP0152185A1 (en) * 1984-01-17 1985-08-21 Ecc International Limited Measurement of physical properties of the solid component of a slurry
DE4041433A1 (de) * 1990-12-21 1992-06-25 Siemens Ag Einrichtung zur aufbereitung von mehrphasengemischen fuer die analyse
EP0583078A3 (en) * 1992-07-14 1994-11-17 Wallac Oy Sample handling apparatus and method and sample collection system.
WO1995027195A1 (en) * 1994-03-31 1995-10-12 Millipore Corporation Membrane cutter and retriever
EP1393079A4 (en) * 2001-05-25 2007-03-14 Proteome Systems Intellectual LIQUID HANDLING AGENT FOR AN EXCISIONING DEVICE
WO2011067309A1 (en) * 2009-12-04 2011-06-09 Whatman International Ltd Methods and systems to prevent punch loss during automated sample processing
CN110005959A (zh) * 2019-04-22 2019-07-12 中国石油天然气集团有限公司 一种输气管道气质分析一体化整合检测装置
US10376692B2 (en) 2002-07-04 2019-08-13 Inovio As Electroporation device and injection apparatus

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US4849077A (en) * 1984-08-06 1989-07-18 Akademie Der Wissenschaften Der Ddr Process for solid phase-sequencing of nucleic acid fragments
US4954319A (en) * 1987-12-16 1990-09-04 Fuji Photo Film Co., Ltd. Mechanism for supplying analytical tape for biochemical analysis by predetermined length
DE4209871C2 (de) * 1991-05-28 1997-04-30 Dade Int Inc Einrichtung zur automatischen Untersuchung von Blutproben
US5210927A (en) * 1991-06-17 1993-05-18 Ways & Means, Inc. Pipette tip filter inserter and method therefore
US5508200A (en) * 1992-10-19 1996-04-16 Tiffany; Thomas Method and apparatus for conducting multiple chemical assays
US5425263A (en) * 1993-06-01 1995-06-20 Barringer Research Limited Method for inspecting an article for concealed substances
TW384398B (en) * 1995-12-19 2000-03-11 Toa Medical Electronics Sample agitating and sucking apparatus
ES2350702T3 (es) * 1998-01-12 2011-01-26 Massachusetts Institute Of Technology Sistema para analizar una pluralidad de muestras.
US6200816B1 (en) * 1998-10-21 2001-03-13 The United States Of America As Represented By The Department Of Energy Method for measuring particulate and gaseous metals in a fluid stream, device for measuring particulate and gaseous metals in a fluid stream
US6465749B1 (en) 2000-07-07 2002-10-15 Los Robles Advertising, Inc. Magnetostrictive mass sensing apparatus and method
AU2001284555A1 (en) * 2000-07-31 2002-02-13 Strategic Technology Limited Sample preparation system
US6958234B2 (en) * 2003-04-03 2005-10-25 Hospira, Inc. Automatic microbial air sampling system and method
US20050218067A1 (en) * 2003-04-10 2005-10-06 Cuno Incorporated Ablated predetermined surface geometric shaped boundary formed on porous material mounted on a substrate and methods of making same
US20070056898A1 (en) * 2004-05-13 2007-03-15 Keith Wesner Ablated predetermined surface geometric shaped boundary formed on porous material mounted on a substrate and methods of making same
GB0421352D0 (en) * 2004-09-24 2004-10-27 City Tech Sampling and analysis system and method
US9485917B2 (en) 2006-12-15 2016-11-08 Ecovative Design, LLC Method for producing grown materials and products made thereby
US20110108495A1 (en) * 2009-11-06 2011-05-12 Matthew Neil Mynott Taylor Specimen Analysis and Extraction System
CN102890015B (zh) * 2012-05-24 2015-05-27 深圳国技仪器有限公司 防尘防倒吸装置
US20140056653A1 (en) * 2012-08-22 2014-02-27 Christopher Scully Method and Machine for Filling 3D Cavities with Bulk Material
US11277979B2 (en) 2013-07-31 2022-03-22 Ecovative Design Llc Mycological biopolymers grown in void space tooling
US20150101509A1 (en) 2013-10-14 2015-04-16 Gavin R. McIntyre Method of Manufacturing a Stiff Engineered Composite
US10537070B2 (en) 2015-04-15 2020-01-21 Ecovative Design Llc Process for the production of mycelial composite surfaces in a roll-to-roll format
CN109153964B (zh) 2016-03-01 2023-06-13 芬德集团公司 丝状真菌生物垫、及其生产方法和使用方法
CA3058212A1 (en) 2017-03-31 2018-10-04 Jessie Hannah Kaplan-Bei Solution based post-processing methods for mycological biopolymer material and mycological product made thereby
US11266085B2 (en) 2017-11-14 2022-03-08 Ecovative Design Llc Increased homogeneity of mycological biopolymer grown into void space
US11920126B2 (en) 2018-03-28 2024-03-05 Ecovative Design Llc Bio-manufacturing process
US11293005B2 (en) 2018-05-07 2022-04-05 Ecovative Design Llc Process for making mineralized mycelium scaffolding and product made thereby
WO2019226823A1 (en) 2018-05-24 2019-11-28 Ecovative Design Llc Process and apparatus for producing mycelium biomaterial
EP3860370A4 (en) 2018-10-02 2022-10-12 Ecovative Design LLC A bioreactor paradigm for the production of secondary extra-particle hyphal matrices

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US3923463A (en) * 1972-10-09 1975-12-02 Kenneth Dawson Bagshawe Apparatus for performing chemical and biological analysis
US4167875A (en) * 1976-08-05 1979-09-18 Meakin John C Filtration method and apparatus

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US3940250A (en) * 1972-01-31 1976-02-24 Vitatect Corporation Testing using luminescent reaction
DE2324056A1 (de) * 1973-05-12 1974-11-21 Faudi Feinbau Schlammtrockenanlage
US4341735A (en) * 1980-03-28 1982-07-27 American Cyanamid Company Sample carrier material handling apparatus
US4395493A (en) * 1981-05-14 1983-07-26 Coulter Electronics, Inc. Monolayer device using filter techniques

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
US3244287A (en) * 1962-12-21 1966-04-05 Coleman Instr Corp Continuous filtration
DE2241646A1 (de) * 1972-08-24 1974-03-28 Friedrich Dr Rer Nat D Erhardt Streifen mit einer reihe radioimmunologischer proben sowie verfahren und vorrichtung zur herstellung eines solchen streifens
US3923463A (en) * 1972-10-09 1975-12-02 Kenneth Dawson Bagshawe Apparatus for performing chemical and biological analysis
US4167875A (en) * 1976-08-05 1979-09-18 Meakin John C Filtration method and apparatus

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0148290A1 (en) * 1983-12-14 1985-07-17 Försvarets Forskningsanstalt Method and device at the analysis of liquid samples
EP0152185A1 (en) * 1984-01-17 1985-08-21 Ecc International Limited Measurement of physical properties of the solid component of a slurry
US4704899A (en) * 1984-01-17 1987-11-10 English Clays Lovering Pochin & Company, Limited Measurement of physical properties of the solid component of a slurry
DE4041433A1 (de) * 1990-12-21 1992-06-25 Siemens Ag Einrichtung zur aufbereitung von mehrphasengemischen fuer die analyse
EP0583078A3 (en) * 1992-07-14 1994-11-17 Wallac Oy Sample handling apparatus and method and sample collection system.
WO1995027195A1 (en) * 1994-03-31 1995-10-12 Millipore Corporation Membrane cutter and retriever
EP1393079A4 (en) * 2001-05-25 2007-03-14 Proteome Systems Intellectual LIQUID HANDLING AGENT FOR AN EXCISIONING DEVICE
US10376692B2 (en) 2002-07-04 2019-08-13 Inovio As Electroporation device and injection apparatus
US11471675B2 (en) 2002-07-04 2022-10-18 Inovio Pharmaceuticals, Inc. Electroporation device and injection apparatus
WO2011067309A1 (en) * 2009-12-04 2011-06-09 Whatman International Ltd Methods and systems to prevent punch loss during automated sample processing
CN110005959A (zh) * 2019-04-22 2019-07-12 中国石油天然气集团有限公司 一种输气管道气质分析一体化整合检测装置

Also Published As

Publication number Publication date
EP0110890B1 (de) 1987-04-22
DE3371119D1 (en) 1987-05-27
CH655392B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1986-04-15
EP0110890A1 (de) 1984-06-20
US4568520A (en) 1986-02-04

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