US8226941B2 - Methods of purifying chondroitinase and stable formulations thereof - Google Patents
Methods of purifying chondroitinase and stable formulations thereof Download PDFInfo
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- US8226941B2 US8226941B2 US11/568,831 US56883105A US8226941B2 US 8226941 B2 US8226941 B2 US 8226941B2 US 56883105 A US56883105 A US 56883105A US 8226941 B2 US8226941 B2 US 8226941B2
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- chondroitinase
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
- C12Y402/02004—Chondroitin ABC lyase (4.2.2.4), i.e. chondroitinase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Definitions
- FIG. 10 Dependence of cABCI thermal stability on ionic strength of in the presence of 50 mM Na Phosphate buffer.
- chondroitinase gene can be produced by ligating a nucleic acid encoding a chondroitinase protein, or a portion thereof, into a vector suitable for expression in either prokaryotic cells, eukaryotic cells, or both. Procedures for ligation are well known to those of ordinary skill in the art.
- Expression vectors for production of recombinant forms of the subject chondroitinase polypeptides include plasmids and other vectors.
- Gel filtration chromatography is a separation based on size. It is also called molecular exclusion or gel permeation chromatography.
- the stationary phase consists of porous beads with a well-defined range of pore sizes.
- the stationary phase for gel filtration is said to have a fractionation range, meaning that molecules within that molecular weight range can be separated.
- the final enzyme yield can be up to about 50 mg chondroitinase from 1 L cultured cells. In a further embodiment the final enzyme yield can be in the range of about 75 to 85 mg/l L of cells.
- a purified formulation of a chondroitinase a recombinant ABCI was purified and characterized using the methods of the present invention using the following parameters: temperature stability, enzyme characteristics, susceptibility to various stress conditions, degradation products; effects of different excipients on enzyme stability.
- the cells expressing chondroitinase AC were extracted using a square tip sonicator at a maximum speed of about 9. Sonication was performed for about 30 seconds. This was immediately followed by about 10 seconds with no sonication. These on/off steps were performed for a total of about 10 cycles. Each pellet was sonicated separately and then pooled. Extractions were rocked overnight at 4° C.
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Biotechnology (AREA)
- Public Health (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/568,831 US8226941B2 (en) | 2004-05-18 | 2005-05-18 | Methods of purifying chondroitinase and stable formulations thereof |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US57203004P | 2004-05-18 | 2004-05-18 | |
| US62188204P | 2004-10-25 | 2004-10-25 | |
| PCT/US2005/017464 WO2005112986A2 (en) | 2004-05-18 | 2005-05-18 | Purifying chondroitinase and stable formulations thereof |
| US11/568,831 US8226941B2 (en) | 2004-05-18 | 2005-05-18 | Methods of purifying chondroitinase and stable formulations thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| US20080311642A1 US20080311642A1 (en) | 2008-12-18 |
| US8226941B2 true US8226941B2 (en) | 2012-07-24 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US11/568,831 Active 2027-12-25 US8226941B2 (en) | 2004-05-18 | 2005-05-18 | Methods of purifying chondroitinase and stable formulations thereof |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US8226941B2 (es) |
| EP (1) | EP1750757B1 (es) |
| JP (1) | JP4926962B2 (es) |
| AU (1) | AU2005244933B2 (es) |
| CA (1) | CA2566731C (es) |
| ES (1) | ES2411504T3 (es) |
| MX (2) | MXPA06013345A (es) |
| WO (1) | WO2005112986A2 (es) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9796970B1 (en) | 2017-04-24 | 2017-10-24 | Advantek Serum Laboratories Ltd. | Production of high purity chondroitinase ABC |
| WO2018200290A1 (en) * | 2017-04-24 | 2018-11-01 | Advantek Serum Laboratories Ltd. | Production of high purity chondroitinase abc |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60336341D1 (de) | 2002-05-04 | 2011-04-21 | Acorda Therapeutics Inc | Zusammensetzungen und verfahren zur förderung des neuronalen wachstums |
| AU2003243396A1 (en) | 2002-06-03 | 2003-12-19 | Massachusetts Institute Of Technology | Rationally designed polysaccharide lyases derived from chondroitinase b |
| US7959914B2 (en) | 2003-05-16 | 2011-06-14 | Acorda Therapeutics, Inc. | Methods of reducing extravasation of inflammatory cells |
| MX348062B (es) | 2003-05-16 | 2017-05-26 | Acorda Therapeutics Inc | Mutantes que degradan proteoglicanos para tratamiento del snc. |
| EP1928490B8 (en) | 2005-09-26 | 2012-10-03 | Acorda Therapeutics, Inc. | Compositions and methods of using chondroitinase abci mutants |
| ES2473610T3 (es) | 2006-10-10 | 2014-07-07 | Acorda Therapeutics, Inc. | Composiciones y métodos de uso de mutantes de condroitinasa ABCI |
| JP2011136911A (ja) * | 2009-12-25 | 2011-07-14 | Tosoh Corp | 甲状腺刺激ホルモンレセプターの安定化方法 |
| AU2013323679A1 (en) * | 2012-09-25 | 2015-05-14 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Treatment of Central Nervous System (CNS) injury |
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2005
- 2005-05-18 EP EP05749189A patent/EP1750757B1/en not_active Not-in-force
- 2005-05-18 US US11/568,831 patent/US8226941B2/en active Active
- 2005-05-18 WO PCT/US2005/017464 patent/WO2005112986A2/en active Application Filing
- 2005-05-18 AU AU2005244933A patent/AU2005244933B2/en not_active Ceased
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- 2005-05-18 ES ES05749189T patent/ES2411504T3/es active Active
- 2005-05-18 MX MXPA06013345A patent/MXPA06013345A/es active IP Right Grant
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Also Published As
| Publication number | Publication date |
|---|---|
| CA2566731A1 (en) | 2005-12-01 |
| EP1750757A2 (en) | 2007-02-14 |
| WO2005112986A2 (en) | 2005-12-01 |
| EP1750757B1 (en) | 2013-03-13 |
| EP1750757A4 (en) | 2010-01-13 |
| AU2005244933B2 (en) | 2011-08-04 |
| ES2411504T3 (es) | 2013-07-05 |
| AU2005244933A1 (en) | 2005-12-01 |
| JP2008505661A (ja) | 2008-02-28 |
| US20080311642A1 (en) | 2008-12-18 |
| WO2005112986A3 (en) | 2009-04-16 |
| MXPA06013345A (es) | 2008-10-31 |
| JP4926962B2 (ja) | 2012-05-09 |
| CA2566731C (en) | 2012-07-24 |
| MX341243B (es) | 2016-08-12 |
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