US4215940A - Optode arrangement - Google Patents
Optode arrangement Download PDFInfo
- Publication number
- US4215940A US4215940A US05/817,109 US81710977A US4215940A US 4215940 A US4215940 A US 4215940A US 81710977 A US81710977 A US 81710977A US 4215940 A US4215940 A US 4215940A
- Authority
- US
- United States
- Prior art keywords
- indicator
- radiation
- light
- chamber
- indicator chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Definitions
- the invention relates to apparatus for the optical measurement of the concentrations in a substance to be analyzed, for example, oxygen in blood.
- the type of apparatus in question usually includes a housing containing a monochromator and a light-measuring unit, and an indicator chamber containing an indicator whose spectral response to the light from the monochromator is dependent upon variations in the concentration of the component of interest.
- the side of the indicator chamber facing the medium to be analyzed is closed off by a membrane which is permeable for the substance of interest.
- the side of the indicator chamber facing the monochromator is closed off by a layer of material through which the radiation of interest can pass (optode).
- the substance to be analyzed may itself respond to the reaction to be measured with a spectral alteration of its own; i.e., the radiation to be measured, namely that attributable to the spectral dependence of the indicator upon the concentration of the component of interest, may have superimposed thereon so-called "background radiation” attributable to the spectral response of the substance to be analyzed itself.
- background radiation attributable to the spectral response of the substance to be analyzed itself.
- this is achieved by so designing the apparatus that the light-measuring unit which receives and measures the radiation emitted from the indicator receives that radiation along a radiation path which is oriented at an angle relative to the direction in which the radiation from the monochromator penetrates the indicator.
- the direction in which the radiation to be measured travels from the indicator to the measuring unit is perpendicular to that in which the monochromator radiation travels through the indicator, and preferably, the monochromator radiation travels through the indicator approximately parallel to the membrane plane of the optode.
- the monochromator radiation is no longer incident upon the substance to be analyzed, and therefore the latter no longer contributes to the radiation detected by the light-measuring unit of the device.
- the light from the monochromator is transmitted into the optode by means of a light-conductor element. This further reduces the effect of background radiation, and makes it possible to introduce the monochromator light into the indicator space using inexpensive means.
- an intermediate optical coupling element is utilized, between the light-conductor element and the optode per se, to properly direct the light into the indicator space. This makes for high efficiency in the use of the light from the monochromator. This efficiency is further increased, if the optode is made of circular configuration, with the intermediate optical coupling element enclosing or surrounding the optode.
- the radius of the optode when the latter is of circular configuration, corresponds to the depth to which the light from the monochromator penetrates into the indicator space. This makes for a very uniform irradiation of the indicator contained within the optode.
- the penetration depth of the monochromator light employed is small, then it is preferred not to make the optode of circular configuration, but instead of elongated configuration. This assures uniform penetration of the indicator by monochromator radiation, and increases the effective measuring surface of the device.
- FIG. 1 is a sectional view through an exemplary embodiment of the inventive construction
- FIG. 2 is a schematic front view of the optode, showing its circular configuration
- FIG. 3 is a shematic front view of an optode which is of elongated, instead of circular configuration.
- An optode 1 is filled with an indicator 12.
- the side of the optode which faces the substance 40 to be analyzed is closed off by a membrane 10 which is permeable for the component 400 of interest.
- the side of the optode which faces the light-measuring unit 9 of the device is closed off by a diaphragm through which the radiation of interest can pass, i.e., in order to reach light-measuring unit 9.
- the substance 40 to be analyzed may, for example, be blood, and the substance 400 of interest oxygen.
- the indicator 12 could, in that case be, for example, pyrene butyric acid.
- the membrane 10 could, for example, be Teflon having a thickness of about 12 microns.
- the required excitation radiation 13 can be furnished using a light source and a conventional monochromator or, as illustrated here, directly from a monochromatic light source 23.
- the wavelength of the excitation light would be 326 manometers, and the wavelength of the light emitted by the indicator would be 395 nanometers.
- the monochromatic excitation radiation 13 is transmitted through a light-conductor element 3 into an intermediate optical coupling element 2, to which the light-conductor element 3 is connected.
- Coupling element 2 can be made of for example transparent plastic and be of circular configuration. The coupling element 2 directs the monochromatic light 13 received via light-conductor element 3 radially inward into the indicator space, from all around the periphery of the indicator space.
- the component 400 of interest carried within the substance 40 to be analyzed in the chamber 4 for the latter in front of the optode, passes through the permeable membrane 10 into the indicator space of the optode and mixes with the indicator 12.
- the indicator 12 undergoes a change of color, or a change in its fluorescence if a fluorescent indicator is being employed.
- the radiation 90 emitted from the indicator 12 towards the light-measuring unit 9 of the device travels toward the latter perpendicular to the direction in which the monochromator radiation 13 sweeps alongside the membrane 10. Accordingly, this radiation does not include spectral (color) components originating from the monochromator radiation 13; neither does it include any radiation components which would in the prior art have developed in the substance 40 to be analyzed itself.
- the chamber 4 for the substance 40 to be analyzed can be consolidated, along with the optode, into a single structural component, as illustrated, utilizing a plate 201 which is non-transmissive for the monochromator light to prevent spillover into the chamber 4, with the two components being joined by screws or clamps 200.
- the radius of the circular optode is equal to the penetration depth of the monochromator light into the indicator space, as shown in FIG. 2. This assures uniform excitation of the indicator 12. If, for example, the penetration depth of the monochromator light employed is low, then to avoid a corresponding reduction in the radius of the circular optode, the optode is preferably of elongated, instead of circular, configuration as shown in FIG. 3, in which case the intermediate optical coupling element 2 of FIG. 1, likewise, would be of elongated instead of circular configuration.
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Spectrometry And Color Measurement (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE2632556 | 1976-07-20 | ||
DE2632556A DE2632556C2 (de) | 1976-07-20 | 1976-07-20 | Lichtzuführung für eine Vorrichtung zur optischen Messung von Stoffkonzentrationen |
Publications (1)
Publication Number | Publication Date |
---|---|
US4215940A true US4215940A (en) | 1980-08-05 |
Family
ID=5983455
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US05/817,109 Expired - Lifetime US4215940A (en) | 1976-07-20 | 1977-07-19 | Optode arrangement |
Country Status (8)
Country | Link |
---|---|
US (1) | US4215940A (sv) |
JP (1) | JPS6058822B2 (sv) |
CH (1) | CH617011A5 (sv) |
DE (1) | DE2632556C2 (sv) |
DK (1) | DK303577A (sv) |
FR (1) | FR2359415A1 (sv) |
GB (1) | GB1581766A (sv) |
SE (1) | SE425438B (sv) |
Cited By (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4401122A (en) * | 1979-08-02 | 1983-08-30 | Children's Hospital Medical Center | Cutaneous methods of measuring body substances |
WO1983003344A1 (en) * | 1982-03-30 | 1983-10-13 | Us Commerce | Fiber optic p¿o2? probe |
US4458686A (en) * | 1979-08-02 | 1984-07-10 | Children's Hospital Medical Center | Cutaneous methods of measuring body substances |
US4548907A (en) * | 1983-09-14 | 1985-10-22 | Allied Corporation | Fluorescent fluid determination method and apparatus |
US4606351A (en) * | 1979-04-14 | 1986-08-19 | Max Planck Gesellschaft Zur Foerderung Der Wissenschaften | Optical indicator device for the remote measurement of physical changes in a test subject |
US4626693A (en) * | 1980-10-06 | 1986-12-02 | The Regents Of The University Of California | Remote multi-position information gathering system and method |
US4635467A (en) * | 1985-05-22 | 1987-01-13 | American Hospital Supply Corporation | Calibration cell for the calibration of gaseous or non-gaseous fluid constituent sensors |
US4799756A (en) * | 1980-10-06 | 1989-01-24 | The Regents Of The University Of California | Remote multi-position information gathering system and method |
US5057431A (en) * | 1980-01-18 | 1991-10-15 | Max Planck Gesellschaft Zur Forderung Der Wissenschaften | Device for optical measuring of physical dimensions and material concentrations |
EP0458900A1 (en) * | 1989-02-17 | 1991-12-04 | FIBERCHEM, INC. (a Delaware corporation) | Reservoir fiber optic chemical sensors |
WO1992003086A1 (en) * | 1990-08-14 | 1992-03-05 | Drexel University | In vivo measurement of oxygen concentration levels |
US5244810A (en) * | 1990-01-12 | 1993-09-14 | Gottlieb Amos J | Analytical method |
US20060204331A1 (en) * | 2005-03-01 | 2006-09-14 | Hall David R | Asphalt Recycling Vehicle |
US20070098496A1 (en) * | 2005-03-01 | 2007-05-03 | Hall David R | Wireless Remote-controlled Pavement Recycling Machine |
US20080003057A1 (en) * | 2006-06-29 | 2008-01-03 | Hall David R | Checking Density while Compacting |
US20080014020A1 (en) * | 2006-07-14 | 2008-01-17 | Hall David R | Fogging System for an Asphalt Recycling Machine |
US20080056822A1 (en) * | 2006-09-06 | 2008-03-06 | Hall David R | Asphalt Reconditioning Machine |
US7585128B2 (en) | 2007-02-13 | 2009-09-08 | Hall David R | Method for adding foaming agents to pavement aggregate |
US7740414B2 (en) | 2005-03-01 | 2010-06-22 | Hall David R | Milling apparatus for a paved surface |
US20110275913A1 (en) * | 2009-01-13 | 2011-11-10 | Smart Medical Solutions Gmbh | Device for measuring at least one parameter of an arterial blood sample |
US8403595B2 (en) | 2006-12-01 | 2013-03-26 | David R. Hall | Plurality of liquid jet nozzles and a blower mechanism that are directed into a milling chamber |
US11029293B2 (en) | 2016-10-18 | 2021-06-08 | Institut National D'optique | Method and system for the detection of a chemical species in solution |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3032150A1 (de) * | 1980-08-26 | 1982-04-01 | Hellige Gmbh, 7800 Freiburg | Verfahren und messeinrichtung zur kolorimetrischen bestimmung der konzentration eines chemischen stoffs, insbesondere des partialdrucks eines im blut geloesten gases |
DE3213183A1 (de) * | 1982-04-08 | 1983-10-20 | Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Anordnung zur optischen messung physikalischer groessen |
DE3222325A1 (de) | 1982-06-14 | 1983-12-15 | Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Verfahren und anordnung zur messung der ionenstaerke |
DE3344019C2 (de) * | 1983-12-06 | 1995-05-04 | Max Planck Gesellschaft | Vorrichtung zur optischen Messung der Konzentration einer in einer Probe enthaltenen Komponente |
SE455537B (sv) * | 1985-01-31 | 1988-07-18 | Bifok Ab | Sett for kemisk analys vid vilken provet och/eller dess reaktionsprodukter bringas att passera en genomstromningscell, samt en apparatur for utovande av settet |
US5601997A (en) * | 1995-02-03 | 1997-02-11 | Tchao; Ruy | Chemotaxis assay procedure |
DE10239204B3 (de) * | 2002-08-21 | 2004-06-09 | Frank Dipl.-Ing. Zahn | Ionenstärke-Sensor |
DE102009035502A1 (de) * | 2009-07-30 | 2011-02-03 | Universitätsklinikum Jena | Verfahren und Vorrichtung zur Erfassung der Bewegung und Anlagerung von Zellen und Partikeln an Zell-, Gewebe- und Implantatschichten bei der Simulation von Flussbedingungen |
DE102019204668A1 (de) * | 2019-04-02 | 2020-10-08 | Qundis Gmbh | Messvorrichtung zur Erfassung von Partikeln in einer Rohrleitung und Rohrleitung |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4003707A (en) * | 1975-02-28 | 1977-01-18 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Method and arrangement for measuring the concentration of gases |
US4022529A (en) * | 1975-12-11 | 1977-05-10 | White John U | Feature extraction system for extracting a predetermined feature from a signal |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE382118B (sv) * | 1970-04-13 | 1976-01-12 | Trw Inc | Sett och apparat for kontinuerligt pavisande av syre i en gasstrom |
US3811777A (en) * | 1973-02-06 | 1974-05-21 | Johnson Res Foundation Medical | Time-sharing fluorometer and reflectometer |
DE2346792C3 (de) * | 1973-09-17 | 1979-09-20 | Draegerwerk Ag, 2400 Luebeck | Einrichtung zum Messen des Sauerstoffpartialdruckes in strömenden Medien |
GB1506017A (en) * | 1974-03-04 | 1978-04-05 | Int Diagnostic Tech | Fluorometric system and method for the detection of biologically derived substances |
-
1976
- 1976-07-20 DE DE2632556A patent/DE2632556C2/de not_active Expired
-
1977
- 1977-07-06 DK DK303577A patent/DK303577A/da not_active Application Discontinuation
- 1977-07-15 GB GB29740/77A patent/GB1581766A/en not_active Expired
- 1977-07-18 CH CH887177A patent/CH617011A5/de not_active IP Right Cessation
- 1977-07-19 US US05/817,109 patent/US4215940A/en not_active Expired - Lifetime
- 1977-07-19 SE SE7708350A patent/SE425438B/sv not_active IP Right Cessation
- 1977-07-19 FR FR7722133A patent/FR2359415A1/fr active Granted
- 1977-07-20 JP JP52087205A patent/JPS6058822B2/ja not_active Expired
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4003707A (en) * | 1975-02-28 | 1977-01-18 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Method and arrangement for measuring the concentration of gases |
US4022529A (en) * | 1975-12-11 | 1977-05-10 | White John U | Feature extraction system for extracting a predetermined feature from a signal |
Cited By (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4606351A (en) * | 1979-04-14 | 1986-08-19 | Max Planck Gesellschaft Zur Foerderung Der Wissenschaften | Optical indicator device for the remote measurement of physical changes in a test subject |
US4401122A (en) * | 1979-08-02 | 1983-08-30 | Children's Hospital Medical Center | Cutaneous methods of measuring body substances |
US4458686A (en) * | 1979-08-02 | 1984-07-10 | Children's Hospital Medical Center | Cutaneous methods of measuring body substances |
US5057431A (en) * | 1980-01-18 | 1991-10-15 | Max Planck Gesellschaft Zur Forderung Der Wissenschaften | Device for optical measuring of physical dimensions and material concentrations |
US4626693A (en) * | 1980-10-06 | 1986-12-02 | The Regents Of The University Of California | Remote multi-position information gathering system and method |
US4799756A (en) * | 1980-10-06 | 1989-01-24 | The Regents Of The University Of California | Remote multi-position information gathering system and method |
WO1983003344A1 (en) * | 1982-03-30 | 1983-10-13 | Us Commerce | Fiber optic p¿o2? probe |
US4476870A (en) * | 1982-03-30 | 1984-10-16 | The United States Of America As Represented By The Department Of Health And Human Services | Fiber optic PO.sbsb.2 probe |
US4548907A (en) * | 1983-09-14 | 1985-10-22 | Allied Corporation | Fluorescent fluid determination method and apparatus |
US4635467A (en) * | 1985-05-22 | 1987-01-13 | American Hospital Supply Corporation | Calibration cell for the calibration of gaseous or non-gaseous fluid constituent sensors |
EP0458900A4 (en) * | 1989-02-17 | 1992-08-26 | Fiberchem, Inc. (A Delaware Corporation) | Reservoir fiber optic chemical sensors |
EP0458900A1 (en) * | 1989-02-17 | 1991-12-04 | FIBERCHEM, INC. (a Delaware corporation) | Reservoir fiber optic chemical sensors |
US5244810A (en) * | 1990-01-12 | 1993-09-14 | Gottlieb Amos J | Analytical method |
US5186173A (en) * | 1990-08-14 | 1993-02-16 | Drexel University | Method for in vivo measurement of oxygen concentration levels |
WO1992003086A1 (en) * | 1990-08-14 | 1992-03-05 | Drexel University | In vivo measurement of oxygen concentration levels |
US20060204331A1 (en) * | 2005-03-01 | 2006-09-14 | Hall David R | Asphalt Recycling Vehicle |
US20070098496A1 (en) * | 2005-03-01 | 2007-05-03 | Hall David R | Wireless Remote-controlled Pavement Recycling Machine |
US7740414B2 (en) | 2005-03-01 | 2010-06-22 | Hall David R | Milling apparatus for a paved surface |
US20080003057A1 (en) * | 2006-06-29 | 2008-01-03 | Hall David R | Checking Density while Compacting |
US7712996B2 (en) | 2006-07-14 | 2010-05-11 | Hall David R | Fogging system for an asphalt recycling machine |
US20080014020A1 (en) * | 2006-07-14 | 2008-01-17 | Hall David R | Fogging System for an Asphalt Recycling Machine |
US20080056822A1 (en) * | 2006-09-06 | 2008-03-06 | Hall David R | Asphalt Reconditioning Machine |
US8403595B2 (en) | 2006-12-01 | 2013-03-26 | David R. Hall | Plurality of liquid jet nozzles and a blower mechanism that are directed into a milling chamber |
US7585128B2 (en) | 2007-02-13 | 2009-09-08 | Hall David R | Method for adding foaming agents to pavement aggregate |
US20110275913A1 (en) * | 2009-01-13 | 2011-11-10 | Smart Medical Solutions Gmbh | Device for measuring at least one parameter of an arterial blood sample |
US9091660B2 (en) * | 2009-01-13 | 2015-07-28 | Smart Medical Solutions Gmbh | Device for measuring at least one parameter of an arterial blood sample |
US11029293B2 (en) | 2016-10-18 | 2021-06-08 | Institut National D'optique | Method and system for the detection of a chemical species in solution |
Also Published As
Publication number | Publication date |
---|---|
GB1581766A (en) | 1980-12-17 |
DK303577A (da) | 1978-01-21 |
SE7708350L (sv) | 1978-01-21 |
DE2632556C2 (de) | 1984-09-20 |
JPS6058822B2 (ja) | 1985-12-21 |
JPS5331183A (en) | 1978-03-24 |
CH617011A5 (sv) | 1980-04-30 |
DE2632556A1 (de) | 1978-01-26 |
FR2359415A1 (fr) | 1978-02-17 |
FR2359415B1 (sv) | 1983-12-23 |
SE425438B (sv) | 1982-09-27 |
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