GB1506017A - Fluorometric system and method for the detection of biologically derived substances - Google Patents

Fluorometric system and method for the detection of biologically derived substances

Info

Publication number
GB1506017A
GB1506017A GB8059/75A GB805975A GB1506017A GB 1506017 A GB1506017 A GB 1506017A GB 8059/75 A GB8059/75 A GB 8059/75A GB 805975 A GB805975 A GB 805975A GB 1506017 A GB1506017 A GB 1506017A
Authority
GB
United Kingdom
Prior art keywords
fluorescence
sample
antibody
light
fluorescent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB8059/75A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
International Diagnostic Technologies Inc
Original Assignee
International Diagnostic Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by International Diagnostic Technologies Inc filed Critical International Diagnostic Technologies Inc
Publication of GB1506017A publication Critical patent/GB1506017A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measurement Of Radiation (AREA)

Abstract

1506017 Fluorometric sample detection INTERNATIONAL DIAGNOSTIC TECHNOLOGY Inc 26 Feb 1975 [4 March 1974] 8059/75 Headings G1A and G1B A fluorometric system comprising means for exciting and means for receiving fluorescent light and a body 10 with an elongate handle joined to a portion having a continuous surface carrying a reagent 11 selectively reactive with the biologically derived substance 14 to be detected. Sample 11 may be an antigen and substance 14 an antibody or vice versa. The body may have two samples one of which is a standard, the intensity of fluorescence being proportional to the quantity of antigen. Alternatively the samples may comprise different antigens (or antibodies), the labels being different fluorescent substances. The sample areas may comprise bands. The fluorometric system may comprise such a body and a monochromatic light source between 0À2 and 1À0Ám and means, as light guide 24, to convey the light to the body of a detector of fluorescence. Nylon sphere 10 of diameter 10mm bears a dried antibody film 11. Glass cuvette is racked for 5 mins with added serum 13 so that antigen 14 present binds to 11. The cuvette is rinsed, incubated with solution of antibody 23 tagged with sodium fluorescein isothyocyanate and resinsed. Ultraviolet light is then passed through filter 28 of 460 nm. Fluorescence from antibody 23 reaches a photo-multiplier via a filter and a fibre optic cable. The electric signal is filtered, rectified, linearized, amplified, converted to digital and displayed on a digital meter calibrated in titre. Two fibre optic cables each with filters and a chopper wheel may supply the photo-multiplier with fluorescence alternatively from the two bands. Two photomultipliers may be used without a chopper. The substrate may be paddle shaped, a cube or other polyhedron with a handle and with a different sample on each of the vertical faces. The fluorometer may have a filter wheel with a selected fluorescence wavelength for each face. A modified fluorometer may have a single fibre optic cable 86 dividing into two or into four as in Fig. 9. Substrate 87 has fluorescent sample 88. Branches 81, 82 transmit light from lamps 89 and 90 and branches 83 and 84 conduct the fluoroescence through lenses 91 and 92 to solid state detectors or photo-multipliers 93, 94. Surface 88 may include a plurality of randomly dispersed fluorescent samples, tagged with different exciting wavelengths and excited from a single lamp via a plurality of filters.
GB8059/75A 1974-03-04 1975-02-26 Fluorometric system and method for the detection of biologically derived substances Expired GB1506017A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US44757274A 1974-03-04 1974-03-04

Publications (1)

Publication Number Publication Date
GB1506017A true GB1506017A (en) 1978-04-05

Family

ID=23776872

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8059/75A Expired GB1506017A (en) 1974-03-04 1975-02-26 Fluorometric system and method for the detection of biologically derived substances

Country Status (5)

Country Link
JP (2) JPS50130496A (en)
CA (1) CA1033587A (en)
DE (2) DE2509215C2 (en)
FR (1) FR2263510B1 (en)
GB (1) GB1506017A (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4719182A (en) * 1985-03-18 1988-01-12 Eastman Kodak Company Fluorescent labels and labeled species and their use in analytical elements and determinations
US4735907A (en) * 1985-03-18 1988-04-05 Eastman Kodak Company Stabilized fluorescent rare earth labels and labeled physiologically reactive species
US4934811A (en) * 1988-04-01 1990-06-19 Syntex (U.S.A.) Inc. Apparatus and method for detection of fluorescence or light scatter
US5013150A (en) * 1988-04-01 1991-05-07 Syntex (U.S.A.) Inc. Method for detection of fluorescence or light scatter
GB2339900A (en) * 1998-07-21 2000-02-09 Cambridge Imaging Ltd Imaging system for luminescence assays
US8323904B2 (en) 2005-12-16 2012-12-04 Bayer Cropscience Ag Kit for measurement of termite insecticide active ingredient by immunoassay method
CN116298324A (en) * 2023-05-25 2023-06-23 武汉大学人民医院（湖北省人民医院） Method, device, equipment and readable storage medium for detecting beta 2-microglobulin

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3999948A (en) * 1975-11-03 1976-12-28 International Diagnostic Technology Diagnostic reagent holder and method
DE2632556C2 (en) * 1976-07-20 1984-09-20 Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Light feed for a device for the optical measurement of substance concentrations
US4294817A (en) * 1977-11-25 1981-10-13 International Diagnostic Technology, Inc. Method of fluoro immunoassay
US4320087A (en) * 1978-01-23 1982-03-16 Abbott Laboratories Laboratory assay device
FR2438844A2 (en) * 1978-10-12 1980-05-09 Int Diagnostic Tech Body for labelled quantitative determn. of biological sample - which facilitates non-touchable handling, reproducible sample detection with low background noise and minimal classification error
CA1148859A (en) * 1979-06-14 1983-06-28 Lacy R. Overby Simultaneous assay of two hepatitis viruses using a solid phase
JPS56157661U (en) * 1980-04-24 1981-11-25
DE3025022C2 (en) * 1980-07-02 1985-07-04 Wang, Wei-Kung, T'ai-pei Method for the determination of biological particles by induced signals
EP0059032A1 (en) * 1981-02-03 1982-09-01 Virgil B. Elings Measurement of dye concentration in the bloodstream
FR2528581A1 (en) * 1982-06-15 1983-12-16 Commissariat Energie Atomique DEVICE FOR THE ASSAY OF A BIOLOGICAL COMPOUND, METHOD FOR MANUFACTURING THE SAME AND USE THEREOF IN A DOSING PROCESS
US4564598A (en) * 1982-07-12 1986-01-14 Syntex (U.S.A.) Inc. Limited volume method for particle counting
US4487839A (en) * 1983-01-05 1984-12-11 Ortho Diagnostic Systems Inc. Immunoassay methods employing patterns for the detection of soluble and cell surface antigens
FR2613074B1 (en) * 1987-03-27 1990-06-08 Commissariat Energie Atomique ACTIVE CHEMICAL SENSOR WITH OPTICAL FIBERS
DE4303858C2 (en) * 1993-02-10 1995-08-31 Draegerwerk Ag Device for the colorimetric detection of gaseous and / or vaporous components of a gas mixture due to the discoloration of a reaction zone arranged in a channel
JP2007187650A (en) * 2005-12-16 2007-07-26 Horiba Ltd Fipronyl measuring kit using immunoassay

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3612866A (en) * 1969-07-08 1971-10-12 Brian Stevens Instrument for determining oxygen quantities by measuring oxygen quenching of fluorescent radiation
US3690769A (en) * 1969-12-12 1972-09-12 Olympus Optical Co Endoscope with built-in oximeter
GB1329823A (en) * 1970-06-09 1973-09-12 Oxoid Ltd Means for carrying out diagnostic tests for infections
US3826619A (en) * 1971-12-21 1974-07-30 Abbott Lab Test apparatus for direct radioimmuno-assay for antigens and their antibodies
US3800161A (en) * 1972-12-19 1974-03-26 Atomic Energy Commission Portable dynamic multistation photometer-fluorometer

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4719182A (en) * 1985-03-18 1988-01-12 Eastman Kodak Company Fluorescent labels and labeled species and their use in analytical elements and determinations
US4735907A (en) * 1985-03-18 1988-04-05 Eastman Kodak Company Stabilized fluorescent rare earth labels and labeled physiologically reactive species
US4934811A (en) * 1988-04-01 1990-06-19 Syntex (U.S.A.) Inc. Apparatus and method for detection of fluorescence or light scatter
US5013150A (en) * 1988-04-01 1991-05-07 Syntex (U.S.A.) Inc. Method for detection of fluorescence or light scatter
GB2339900A (en) * 1998-07-21 2000-02-09 Cambridge Imaging Ltd Imaging system for luminescence assays
GB2339900B (en) * 1998-07-21 2003-04-23 Cambridge Imaging Ltd Improved imaging system for luminescence Assays
US8323904B2 (en) 2005-12-16 2012-12-04 Bayer Cropscience Ag Kit for measurement of termite insecticide active ingredient by immunoassay method
CN116298324A (en) * 2023-05-25 2023-06-23 武汉大学人民医院（湖北省人民医院） Method, device, equipment and readable storage medium for detecting beta 2-microglobulin

Also Published As

Publication number Publication date
DE2509215C2 (en) 1983-11-03
JPS50130496A (en) 1975-10-15
JPS6132621B2 (en) 1986-07-28
FR2263510A1 (en) 1975-10-03
FR2263510B1 (en) 1979-02-16
CA1033587A (en) 1978-06-27
JPS55121135A (en) 1980-09-18
DE2560288C2 (en) 1984-06-20
DE2509215A1 (en) 1975-10-30

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Legal Events

Date Code Title Description
PS Patent sealed
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19930226