US3928136A - Container for collection of cultures of parasitic fungi and a method of using the same - Google Patents

Container for collection of cultures of parasitic fungi and a method of using the same Download PDF

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Publication number
US3928136A
US3928136A US406885A US40688573A US3928136A US 3928136 A US3928136 A US 3928136A US 406885 A US406885 A US 406885A US 40688573 A US40688573 A US 40688573A US 3928136 A US3928136 A US 3928136A
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Prior art keywords
vessel
container
cover
chamber
flat face
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Expired - Lifetime
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US406885A
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English (en)
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Pierre A Launey
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Definitions

  • Field of Search 195/ 101, 127, 139, 54 transparent plastic, and comprises a vessel having in 195/77, I02; 229/25, 43; 220/42 A; 206/84, its upper face an elongated depression of semicylindri- 523; 23/253 TP, 259 cal cross-section.
  • a cover enables the vessel to be hermetically sealed by a method eliminating the risk of [56] References Cited internal condensation despite the humidity of the agar UNTTED STATES PATENTS on which the parasite is cultivated. 2,677,647 5/1954 Lovell 195/139 9 Claims, 3 Drawing Figures US. Patent Dec. 23, 1975 3,928,136
  • the invention relates to a container permitting the formation of a collection of cultures, interrupted at different stages of evolution, of parasites of a fungal nature. These parasites are those which can be collected from the skin or any other similar surface region of man, animals, and vegetables.
  • the main object of the invention is to provide a container for use in making up a collection of parasites arrested at a particular stage of their growth, which will permit easy observation with a binocular glass.
  • Subsidiary objects are to present the parasites with the usual appearance which they present in test tubes, and to preserve the parasites without alteration despite the fact that the agar serving as support retains a minimum degree of humidity.
  • a container for the collection of parasites of a fungal nature comprises a vessel having a flat face in which is formedan elongated chamber of semicylindrical'cross;section bounded by transparent material, and a closure cover'adapted to cover the flat face of the vessel and to be hermetically joined to the vessel.
  • the elongated chamber may contain agar serving as support for a parasite of a fungal nature which has been arrested in its growth at any desired stage, the cover being sealed to the vessel by a method known per se such as adhesive bonding and welding.
  • a fungal parasite is seeded in the agar and allowed to develop to the desired stage;
  • the open container is placed in a chamber at 30C for 3 hours;
  • the container is closed with the aid of its cover in an atmosphere of dry air at 30C;
  • the container and the parasite are subjected to a flux of gamma rays from a radioactive source.
  • a parasitic fungus is seeded in the agar and allowed to develop to the desired stage;
  • the open container is placed in a chamber at 30C for 3 hours;
  • the container is introduced into a chamber containing a sterilizing gas atmosphere, and then the cover is placed in position and hermetically sealed.
  • FIG. 1 is a view in perspective of a collection container according to the invention, comprising a vessel and a cover,
  • FIGS. 2 and 3 are respectively views in longitudinal section and cross-section of the vessel of FIG. 1.
  • a culture collection container comprises a vessel 1 which has an elongated chamber 2 (FIG. 2) having a semicylindrical cross-section (FIG. 3), and which has a flat top face 3.
  • the elongated semicylindrical chamber 2 is bounded by a transparent material and opens at to the flat fact 3.
  • the container also comprises a cover 4 adapted to cover the flat face 3 of the vessel 1 and to be joined hermetically to the vessel.
  • the vessel as can be seen in FIGS. 1 to 3, to be composed of a wall forming five faces of a hollow parallelepiped, namely a top face (the flat face 3) and four side faces extending from the top face.
  • the elongated chamber 2 is formed by a depression having the abovedescribed section and formed in the top face. This depression extends toa depth which is less than the height of the side walls. Thus, the vessel can stand on the free bottom edge of these walls.
  • the free bottom edge of the side walls may have an outwardly projecting rim 5 and for the cover to extend as far as this rim.
  • the latter may have a groove 6 intended to receive thebottom edge of the fluid-tight cover, thus facilitating the sealing of the latter to the vessel.
  • bottom edges of the side walls of the vessel and the bottom edge of the cover may as an alternative be provided with corresponding flanges surrounding the vessel and adapted to be adhesively bonded or welded together by known methods.
  • the vessel and the cover of each container are made of transparent material, and it is interesting to note that, as will be explained later, it hasbeen found that a plastic material, such as polystyrene, is particularly suitable.
  • the procedure is as follows.
  • the necessary amount of agar is placed in the elongated chamber of the vessel.
  • a parasitic fungus is seeded and allowed to develop to the desired stage, for example until the characteristic appearance of its species is achieved. Its development is then arrested by sterilizing treatment and the cover is sealed on the vessel.
  • the open contnainer is placed in a chamber at 30C for 3 hours, it is closed by means of its cover in an atmosphere of dry air at 30C and the cover of the container is then sealed.
  • the parasite is killed by exposing it together with the container to a flux of gamma rays from a radioactive source.
  • Glass is not suitable for this process, because it is modified by gamma rays; it is therefore preferable to make the container of polystyrene.
  • a container according to the invention which has been prepared in this manner preferably has a utilizable capacity the cross-section of which is that of a semicylinder of a radius of about 9 mm and a length of about 70 mm. Because of this shape, when the container is observed from the side of its curved face the parasite is seen to have a very similar appearance to that usually observed through a test tube. On the other hand, observation through the flat face 3 of the sealed cover makes it possible for a magnifying instrument to be used.
  • a containerfor the collection of cultures of parasites of a fungal nature comprising a vessel and a cover, both constituted of transparent material, said 5 vessel comprising a body having a flat face, said body having an elongated chamber of semicylindrical crosssection which is open at said flat face, said body including opposite side walls, between which the chamber is disposed, said side walls extending from said flat face in the same direction as said chamber and over a distance greater than the depth of the chamber, said cover being constructed to cover the flat face of the vessel and to be hermetically joined to the vessel.
  • a container according to claim I wherein the elongated chamber contains agar serving as a support for a parasite of a fungal nature which has been arrested in its growth at any desired stage, the cover being hermetically sealed to the vessel.
  • a container according to claim 1, wherein the vessel and its cover are made of polystyrene.
  • a container according to claim 1 wherein the side walls are four in number arranged in opposed pairs which depend from said flat face along four side edges thereof.
  • a method of using containers according to claim I, to form a collection of parasites of a fungal nature comprising placing a necessary amount of agar in the chamber of the vessel of each container;
  • a method of using containers according to claim 1, to form a collection of cultures of parasites of a fungal nature comprising placing a necessary amount of agar in the chamber of the vessel of each container;

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
US406885A 1972-10-26 1973-10-16 Container for collection of cultures of parasitic fungi and a method of using the same Expired - Lifetime US3928136A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7237979A FR2204689B1 (enExample) 1972-10-26 1972-10-26

Publications (1)

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US3928136A true US3928136A (en) 1975-12-23

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US406885A Expired - Lifetime US3928136A (en) 1972-10-26 1973-10-16 Container for collection of cultures of parasitic fungi and a method of using the same

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US (1) US3928136A (enExample)
FR (1) FR2204689B1 (enExample)
GB (1) GB1442348A (enExample)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4179266A (en) * 1977-04-22 1979-12-18 Lukacsek Patricia A Method and apparatus for culturing and examining fungi
US4271270A (en) * 1977-10-27 1981-06-02 Lukacsek Patricia A Apparatus for culturing and examining fungi
US4656130A (en) * 1985-03-14 1987-04-07 Yissum Research Development Company Collagen coated cell growth plates
US4728607A (en) * 1984-03-22 1988-03-01 J. K. And Susie L. Wadley Research Institute And Blood Bank Miniaturized yeast identification system
US4847128A (en) * 1984-03-22 1989-07-11 Wadley Technologies, Inc. Miniaturized yeast identification system
US5081033A (en) * 1984-03-22 1992-01-14 Wadley Technologies, Inc. Miniaturized yeast identification system
US5134064A (en) * 1986-02-21 1992-07-28 Valio Meijerien Keskusosuusliike Method for the determination of microbe concentrations by means of a plating method
US5202262A (en) * 1984-01-31 1993-04-13 Millipore Corporation Apparatus for microbiological testing of liquids
WO1997000002A2 (en) 1995-06-16 1997-01-03 Rcr Scientific, Inc. Method and apparatus for coating containers
US20080160502A1 (en) * 2006-08-10 2008-07-03 Barnes Allen C Portable biological testing device and method
CN104472331A (zh) * 2014-12-01 2015-04-01 西北农林科技大学 用于观察植物根系真菌的快速透明方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2436084A1 (fr) * 1978-09-13 1980-04-11 Manudo Emballage etanche en matiere synthetique

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2677647A (en) * 1952-10-25 1954-05-04 Lovell Chemical Company Pocket incubator

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2677647A (en) * 1952-10-25 1954-05-04 Lovell Chemical Company Pocket incubator

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4179266A (en) * 1977-04-22 1979-12-18 Lukacsek Patricia A Method and apparatus for culturing and examining fungi
US4271270A (en) * 1977-10-27 1981-06-02 Lukacsek Patricia A Apparatus for culturing and examining fungi
US5202262A (en) * 1984-01-31 1993-04-13 Millipore Corporation Apparatus for microbiological testing of liquids
US4728607A (en) * 1984-03-22 1988-03-01 J. K. And Susie L. Wadley Research Institute And Blood Bank Miniaturized yeast identification system
US4847128A (en) * 1984-03-22 1989-07-11 Wadley Technologies, Inc. Miniaturized yeast identification system
US5081033A (en) * 1984-03-22 1992-01-14 Wadley Technologies, Inc. Miniaturized yeast identification system
US4656130A (en) * 1985-03-14 1987-04-07 Yissum Research Development Company Collagen coated cell growth plates
US5134064A (en) * 1986-02-21 1992-07-28 Valio Meijerien Keskusosuusliike Method for the determination of microbe concentrations by means of a plating method
WO1997000002A2 (en) 1995-06-16 1997-01-03 Rcr Scientific, Inc. Method and apparatus for coating containers
US5698260A (en) * 1995-06-16 1997-12-16 Rcr Scientific, Inc. Method and apparatus for coating containers
US20080160502A1 (en) * 2006-08-10 2008-07-03 Barnes Allen C Portable biological testing device and method
US7910361B2 (en) 2006-08-10 2011-03-22 Barnes Allen C Portable biological testing device and method
US20110143388A1 (en) * 2006-08-10 2011-06-16 Barnes Allen C Method of providing portable biological testing capabilities
US8518637B2 (en) 2006-08-10 2013-08-27 Allen C. Barnes Method of providing portable biological testing capabilities
CN104472331A (zh) * 2014-12-01 2015-04-01 西北农林科技大学 用于观察植物根系真菌的快速透明方法
CN104472331B (zh) * 2014-12-01 2016-11-23 西北农林科技大学 用于观察植物根系真菌的快速透明方法

Also Published As

Publication number Publication date
DE2353273B2 (de) 1976-06-24
FR2204689A1 (enExample) 1974-05-24
DE2353273A1 (de) 1974-05-16
GB1442348A (en) 1976-07-14
FR2204689B1 (enExample) 1975-01-03

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