US3735570A - Chromatographic column - Google Patents

Chromatographic column Download PDF

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Publication number
US3735570A
US3735570A US00005261A US3735570DA US3735570A US 3735570 A US3735570 A US 3735570A US 00005261 A US00005261 A US 00005261A US 3735570D A US3735570D A US 3735570DA US 3735570 A US3735570 A US 3735570A
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US
United States
Prior art keywords
core
section
chromatographic column
cross
passage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US00005261A
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English (en)
Inventor
S Bergen
H Dubsky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pye Electronic Products Ltd
Original Assignee
Pye Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pye Ltd filed Critical Pye Ltd
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Publication of US3735570A publication Critical patent/US3735570A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6069—Construction of the column body with compartments or bed substructure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6034—Construction of the column joining multiple columns
    • G01N30/6043—Construction of the column joining multiple columns in parallel

Definitions

  • a chromatographic column having an elongate passage of substantially uniform cross-section and a core of substantially uniform cross-section situated in said elongate passage, the cross-section of said core being such that it contacts the wall of said elongate passage at a plurality of points to form a plurality of substantially separate passages which extend along said elongate passage, the surface of said core constituting, or being coated with, at least in part, a partitioning agent.
  • the core is preferably of square cross-section but can alternatively be of other cross-section, e.g., triangular, pentagonal or star-shaped.
  • the core can be a wire of the desired cross-section and of a material such as stainless steel, as can the tube, although other materials, such as thermoplastics, can be employed for both the tube. and core.
  • the core surface is preferably roughened, by either mechanical or chemical means, to provide a greater surface area for interchange than would otherwise be the case, and particularly to facilitate coating with a stationary phase material.
  • the internal diameter of the tube is preferably between 0.5 and 1.02 millimeters with the core dimensions chosen to make the core an interference fit within the tube.
  • the invention provides a method of manufacturing a partitioning column for chromatography, including the steps of inserting into an elongate passage of substantially uniform crosssection a core of substantially uniform cross-section such that the cross-section of said core contacts the wall of said elongate passage at a plurality of points thereby forming a corresponding plurality of substantially separate passages extending along said column.
  • the core can be drawn into the tube by means of a draw-wire, of smaller diameter than the tube, which is attached to one end of the core.
  • the surface of the core is preferably roughened and coated with a stationary phase material.
  • the FIGURE illustrates a cross section of the column and core.
  • a column for use in gas chromatography comprises a tube 1 of stainless steel, with an internal diameter of 0.609 mm containing a core 2 of stainless steel wire of square section-of side 0.406 mm.
  • the diagonal dimension of the wire section is'slightly greater than the internal diameter of the tube and the core, therefore, is an interference fit within the tube.
  • the surface of the core is coated with a suitable stationary phase 3, for example, squalane.
  • a suitable stationary phase for example, squalane.
  • the core surface is roughened by sand or metal blasting or etching.
  • the increased surface area obtained this way can be three times or more that of the wire surface when smooth.
  • the roughened surface enables a more uniform coating of stationary phase to be applied to the core and avoids a fall of efficiency due to surface tension of the stationary phase causing formation of drops and leaving certain areas uncoated. It also enables a larger volume of stationary phase mate rial to be employed without an increase in the thickness of the stationary phase coating.
  • Another advantage obtained by roughening the surface of the core is that the core may be treated with an active substance of the type normally used as a stationary phase in packed columns, e.g., graphite, porapak, or silicagel.
  • a column of the form shown is manufactured by first roughening the surface of the core wire 2, coating it with a suitable stationary phase material 3 according to known techniques, attaching a draw-wire of smaller diameter than the bore of the tube 1 to one end of the core 2 and passing the draw-wire through the tube. The core wire 1 is then drawn through the tube 2 to make an interference fit therein.
  • a chromatographic column having an elongate tubular passage of substantially uniform cross-section and a core of substantially uniform cross-section situated in said elongate passage, the cross-section of said core being such that it contacts the wall of said elongate passage at a plurality of points to form a plurality of substantially separate passages which extend along said elongate passage, said passage and said core having dimensions making the core an interference fit within said passage, the surface of said core constituting, at least in part, a partitioning agent.
  • a chromatographic column according to claim 1 in which the cross-section of said core is a square and the cross-section of said elongate passage is substantially circular.
  • a chromatographic column according to claim 1 in which said elongate passage is formed by an elongate stainless steel tube.
  • a chromatographic column according to claim 1 in which the cross-section of said elongate passage is substantially circular with an internal diameter which is not less than 0.5 millimeters and not more than 1.02

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
US00005261A 1969-01-23 1970-01-23 Chromatographic column Expired - Lifetime US3735570A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB393669 1969-01-23

Publications (1)

Publication Number Publication Date
US3735570A true US3735570A (en) 1973-05-29

Family

ID=9767671

Family Applications (1)

Application Number Title Priority Date Filing Date
US00005261A Expired - Lifetime US3735570A (en) 1969-01-23 1970-01-23 Chromatographic column

Country Status (6)

Country Link
US (1) US3735570A (enExample)
DE (1) DE2001853A1 (enExample)
FR (1) FR2028987A1 (enExample)
GB (1) GB1238733A (enExample)
NL (1) NL7000676A (enExample)
SE (1) SE355865B (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3866308A (en) * 1973-03-30 1975-02-18 Said Halasz By Said Asshauer E Method of making a column for chromatography
US4872979A (en) * 1987-04-22 1989-10-10 The Perkin-Elmer Corporation Chromatography column

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2409786A1 (fr) * 1977-11-29 1979-06-22 Moreaux Claude Colonne capillaire pour chromatographie en phase gazeuse

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2920478A (en) * 1957-06-24 1960-01-12 Perkin Elmer Corp Vapor fractometer column
US3038326A (en) * 1958-06-02 1962-06-12 Phillips Petroleum Co Chromatographic analyzer

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2920478A (en) * 1957-06-24 1960-01-12 Perkin Elmer Corp Vapor fractometer column
US3038326A (en) * 1958-06-02 1962-06-12 Phillips Petroleum Co Chromatographic analyzer

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3866308A (en) * 1973-03-30 1975-02-18 Said Halasz By Said Asshauer E Method of making a column for chromatography
US4872979A (en) * 1987-04-22 1989-10-10 The Perkin-Elmer Corporation Chromatography column

Also Published As

Publication number Publication date
SE355865B (enExample) 1973-05-07
GB1238733A (enExample) 1971-07-07
DE2001853A1 (de) 1970-08-27
NL7000676A (enExample) 1970-07-27
FR2028987A1 (enExample) 1970-10-16

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