US3701717A - Substrate-containing test container for the cultivation and inspection of micro-organisms - Google Patents

Substrate-containing test container for the cultivation and inspection of micro-organisms Download PDF

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Publication number
US3701717A
US3701717A US13937A US3701717DA US3701717A US 3701717 A US3701717 A US 3701717A US 13937 A US13937 A US 13937A US 3701717D A US3701717D A US 3701717DA US 3701717 A US3701717 A US 3701717A
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US
United States
Prior art keywords
substrate
tube
cultivation
container
organisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US13937A
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English (en)
Inventor
Helmuth Ingvorsen
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Individual
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Individual
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Filing date
Publication date
Priority claimed from GB4412769A external-priority patent/GB1243750A/en
Application filed by Individual filed Critical Individual
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Publication of US3701717A publication Critical patent/US3701717A/en
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Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/20—Baffles; Ribs; Ribbons; Auger vanes

Definitions

  • Substrate-containing test container for the cultivation and inspection of micro-organisms comprising a coating of said substrate on the inner surface of the side walls of said container and an insert means for supporting said coating.
  • This invention relates to a substrate-containing test container and particularly a tubular container for the cultivation and inspection of micro-organisms e.g. bacteria or living cells.
  • Petri dishes It is well known to cultivate micro-organisms in flat circular dishes e.g. Petri dishes, on a substrate such as agar.
  • substrate-containing Petri dishes present large surfaces available for cultivation and therefore the micro organisms growing on a substrate in such Petri dishes can be easily inspected. Therefore, such dishes have found widespread use in clinics, hospitals etc., in which they are used for determining by cultivation the amount and type of bacteria contained in biological fluids.
  • the bacteria cultivated in such Petri dishes are normally studied and counted by means of a microscope.
  • substrate-containing Petri dishes are diflicult to pack in such a manner that they can be sent from one place to another for example by normal post without the risk of breaking such containers during transportation.
  • the object of the invention is to provide a simple and inexpensive substrate-containing container presenting a large free surface available for the cultivation of microorganisms.
  • a substrate-containing container having the inner surface of the side walls coated with a substrate layer.
  • both the bottom and the side walls are coated with said substrate layer.
  • the substrate layer may be applied to the inner surface of the container side walls in several Ways.
  • One of the most simple ways consists in introducing a liquid substrate into the container and rotating the container about its longitudinal axis. When the fluid substrate has been uniformly distributed over the inner surface, the container is cooled to solidify the substrate.
  • the application of the substrate layer can also be effected by introducing a liquid substrate in the container and by moving said container in such a path that the fluid substrate is distributed in an even layer on the inner surfaces. The container is then cooled to solidify the substrate.
  • the substrate layer may also be applied by molding by means of a core which is inserted in the container and is removed after the solidification of the substrate.
  • the substrate layer may be applied by spraying a warm fluid substrate onto the inner surfaces of the container so as to build up a uniform layer on said surfaces.
  • the substrate applied may be solidified by directing a cooling medium for example a stream of cold air onto the exterior side of the container.
  • a further method of applying the substrate on the inner surface of a tube consists in extruding said layer directly onto said surface.
  • the adherence between the tube walls and the substrate layer is insufficient to prevent the substrate layer from being released from the side walls and fall down onto the bottom of the tube. Furthermore, since some micro-organisms liquefy the substrate during their growth it has been found diflicult to maintain a uniform substrate layer on the side walls during the cultivation.
  • This insert means may comprise a number of longitudinal ribs which are connected by spaced transverse ribs so as to form rectangular openings. These ribs are preferably located in such a manner that narrow openings are formed between the side walls and the exterior sides of said ribs.
  • the area of said openings is for Example 1 cm.
  • the insert means can be manufactured in one step and consequently no welding operations are required. After the production of the tubes and the insert means the latter are inserted in the tubes and a substrate layer is applied onto the inner surface of the side walls of the tube.
  • the substrate-containing tube is preferably provided with a screw cap having on its interior side an absorbing pad.
  • This absorbing pad serves to absorb residual liquid present in the substrate-containing tube when a liquid sample, for example urine, has been brought into contact with the substrate and subsequently has been removed from the tube.
  • the insert means preferably comprises an inwardly extending collar provided at its upper end. The exterior side of this collar may be firmly attached to the test tube.
  • the insert means preferably also comprises a bottom plate having a diameter which is equal to that of the tube.
  • FIG. 1 shows a longitudinal sectional view of an embodiment of a substrate-containing container according to the invention
  • FIG. 2 shows a side view of a transparent substratecontaining tube accordance to the invention
  • FIG. 3 shows an enlarged cross sectional view of the substrate-containing tube shown in 'FIG. 1
  • I FIG. 4 shows an enlarged View of an insert means according to the invention.
  • a substrate tube e.g. made from glass or a plastic material, comprises a substrate layer 2 applied onto the inner surfaces of the side walls and the bottom of said tube.
  • the substrate tube shown in FIGS. 2 and 3 comprises a plastic tube 3, having a screw cap 4.
  • the crossing ribs 7 and 8 form openings 10 which at the lower end of the substrate tube are almost quadratic and have an area of 1 cm.
  • the ribs 7 and 8 do not contact the inner surface of the side walls and consequently 4 the liquid substrate medium which is introduced into the tube is permitted to flow in the longitudinal direction as well as transverse thereto so as to form upon cooling a uniform substrate layer 11 firmly anchored to said ribs.
  • a test tube for the cultivation and investigation of micro-organisms having a film of a solid substrate on the inner surface of the tube, and an insert means within the tube and supporting the substrate film, said insert means comprising ribs extending longitudinally of the tube and ribs extending transversely thereto and connecting said longitudinally extending ribs, said ribs being disposed inwardly of the inner surface of the tube so as to form narrow spaces between the outer side of the ribs and the inner surface of the tube, said insert means further comprisinga bottom plate and an annular flange, said plate and flange extending slightly outwardly of said ribs thereby serving to center said insert means in the tube.

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  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
US13937A 1969-02-26 1970-02-25 Substrate-containing test container for the cultivation and inspection of micro-organisms Expired - Lifetime US3701717A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1037369 1969-02-26
GB4412769A GB1243750A (en) 1969-02-26 1969-09-05 Substrate-containing test container for the cultivation and inspection of micro-organisms

Publications (1)

Publication Number Publication Date
US3701717A true US3701717A (en) 1972-10-31

Family

ID=26247473

Family Applications (1)

Application Number Title Priority Date Filing Date
US13937A Expired - Lifetime US3701717A (en) 1969-02-26 1970-02-25 Substrate-containing test container for the cultivation and inspection of micro-organisms

Country Status (7)

Country Link
US (1) US3701717A (ja)
JP (1) JPS502037B1 (ja)
CA (1) CA944707A (ja)
DE (1) DE2009108A1 (ja)
DK (1) DK128357B (ja)
FI (1) FI47379C (ja)
FR (1) FR2035725A5 (ja)

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3849256A (en) * 1972-01-31 1974-11-19 F Linder Substrate-containing test container
US4690896A (en) * 1984-03-26 1987-09-01 Madaus & Co. Device for the determination of micro-organisms
US4840908A (en) * 1988-06-29 1989-06-20 Becton, Dickinson And Company Culture bottle assembly
US4912048A (en) * 1987-12-21 1990-03-27 Difco Laboratories Fluted culture vessel
US5151366A (en) * 1991-05-24 1992-09-29 Invitro Scientific Products, Inc. Cell culture flask
US5272084A (en) * 1991-12-18 1993-12-21 Corning Incorporated Cell culture vessels having interior ridges and method for cultivating cells in same
US20040191895A1 (en) * 2002-09-20 2004-09-30 Whitley Kenneth W. Roller bottle
US11608486B2 (en) 2015-07-02 2023-03-21 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11613727B2 (en) 2010-10-08 2023-03-28 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US11634677B2 (en) 2016-06-07 2023-04-25 Terumo Bct, Inc. Coating a bioreactor in a cell expansion system
US11667876B2 (en) 2013-11-16 2023-06-06 Terumo Bct, Inc. Expanding cells in a bioreactor
US11667881B2 (en) 2014-09-26 2023-06-06 Terumo Bct, Inc. Scheduled feed
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11795432B2 (en) 2014-03-25 2023-10-24 Terumo Bct, Inc. Passive replacement of media
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS52137444U (ja) * 1976-04-13 1977-10-19
DE19607428C2 (de) * 1996-02-28 1999-01-28 Daimler Benz Ag Innenraumverkleidungsteil aus Kunststoff

Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3849256A (en) * 1972-01-31 1974-11-19 F Linder Substrate-containing test container
US4690896A (en) * 1984-03-26 1987-09-01 Madaus & Co. Device for the determination of micro-organisms
US4912048A (en) * 1987-12-21 1990-03-27 Difco Laboratories Fluted culture vessel
US4840908A (en) * 1988-06-29 1989-06-20 Becton, Dickinson And Company Culture bottle assembly
US5151366A (en) * 1991-05-24 1992-09-29 Invitro Scientific Products, Inc. Cell culture flask
US5272084A (en) * 1991-12-18 1993-12-21 Corning Incorporated Cell culture vessels having interior ridges and method for cultivating cells in same
US20040191895A1 (en) * 2002-09-20 2004-09-30 Whitley Kenneth W. Roller bottle
US7449331B2 (en) 2002-09-20 2008-11-11 Whitley Kenneth W Roller bottle
US11773363B2 (en) 2010-10-08 2023-10-03 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11613727B2 (en) 2010-10-08 2023-03-28 Terumo Bct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11746319B2 (en) 2010-10-08 2023-09-05 Terumo Bct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
US11708554B2 (en) 2013-11-16 2023-07-25 Terumo Bct, Inc. Expanding cells in a bioreactor
US11667876B2 (en) 2013-11-16 2023-06-06 Terumo Bct, Inc. Expanding cells in a bioreactor
US11795432B2 (en) 2014-03-25 2023-10-24 Terumo Bct, Inc. Passive replacement of media
US11667881B2 (en) 2014-09-26 2023-06-06 Terumo Bct, Inc. Scheduled feed
US11608486B2 (en) 2015-07-02 2023-03-21 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11634677B2 (en) 2016-06-07 2023-04-25 Terumo Bct, Inc. Coating a bioreactor in a cell expansion system
US11999929B2 (en) 2016-06-07 2024-06-04 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11702634B2 (en) 2017-03-31 2023-07-18 Terumo Bct, Inc. Expanding cells in a bioreactor
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion

Also Published As

Publication number Publication date
FR2035725A5 (ja) 1970-12-18
FI47379B (ja) 1973-07-31
CA944707A (en) 1974-04-02
FI47379C (fi) 1973-11-12
JPS502037B1 (ja) 1975-01-23
DK128357B (da) 1974-04-16
DE2009108A1 (de) 1970-09-17

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