US3586484A - Multistation analytical photometer and method of use - Google Patents

Multistation analytical photometer and method of use Download PDF

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Publication number
US3586484A
US3586484A US827185A US3586484DA US3586484A US 3586484 A US3586484 A US 3586484A US 827185 A US827185 A US 827185A US 3586484D A US3586484D A US 3586484DA US 3586484 A US3586484 A US 3586484A
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United States
Prior art keywords
chambers
cuvettes
samples
transfer disc
transfer
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Expired - Lifetime
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US827185A
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English (en)
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Norman G Anderson
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US Atomic Energy Commission (AEC)
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US Atomic Energy Commission (AEC)
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/111666—Utilizing a centrifuge or compartmented rotor

Definitions

  • a light source and detector are aligned with transparent windows in the cuvettes to determine chemical species concentrations by light absorbancy in the samples contained in the cuvettes.
  • Means are provided for receiving the output of the detector and individually indicating the phototransmittance of samples within the cuvettes.
  • the invention described herein relates generally to photometers and more particularly to an analytical photometer for simultaneously determining the presence of a common substance in a multiplicity of samples wherein precipitates are removed from the samples prior to their transfer to respective cuvettes. It was made in the course of, or under, a contract with the US. Atomic Energy Commission.
  • Protein-free filtrates are required for a variety of purposes including the estimation of acid-soluble nucleotides, free amino acids, and for a number of clinical measurements including blood sugar.
  • the precipitant employed and the ratio between sample and reagent may vary widely in such tests and measurements.
  • a sensitive test for complete precipitation is the absorbance of the supernatant in the ultraviolet. Incompletely sedimented protein contributes both absorption and scatter rendering such test inaccurate.
  • Another object of the invention is to provide a centrifuge rotor wherein sample liquids and reagents which form precipitates may be mixed and centrifuged in a single system to provide a precipitate-free supernatant.
  • an analytical photometer wherein precipitates may be removed from a multiplicity of discrete samples by centrifugation prior to transfer of the samples to cuvettes in a rotary cuvette system.
  • a central transfer disc is provided with a first series of chambers which separately retain precipitating solutions while at rest, and release the solutions to respective sedimentation chambers upon rotation.
  • a third series of chambers receives the supernatant from respective sedimentation chambers by gravity flow when the transfer disc and cuvette system are brought to rest. The supernatant may then be transferred centrifugally to respective cuvettes in the rotary cuvette system surrounding the transfer disc by rotating the disc and cuvette system.
  • Such arrangement facilitates the preparation and photometric measurement of precipitate-free supernatants.
  • FIG. 1 schematically illustrates a transfer disc and photometric system designed in accordance with the present invention.
  • FIGS. 2 through 6 illustrate the stepwise operation of the transfer disc shown in FIG. 1 in mixing, sedimenting and transferring liquid solutions.
  • FIG. 1 schematically illustrates an analyzer incorporating a transfer disc made in accordance with the invention.
  • a pancake-shaped rotor assembly 1 comprises a bolt-flanged steel rotor body 2, glass rings 3 and 4, a slotted polytetrafluorethylene cuvette ring 5, polytetrafluorethylene retaining rings 6 and 7, and a steel bolted flange ring 8. Rings 3, 4, 5, 6 and 7 are compressed between rotor body 2 and flange ring 8 to form a multiplicity of radially oriented cuvettes 9 in slotted cuvette ring 5.
  • Spaced holes 10, axially aligned with cuvettes 9, are provided in rotor body 2, retaining rings 6 and 7, and flange ring 8 so as to provide axially extending passageways permitting passage of a light beam through the cuvettes.
  • a two-piece, centrally positioned removable transfer disc 11 is provided with sets of chambers 12 for receiving sample liquids and reactants while the rotor is at rest.
  • Chambers 12 comprise a plurality (only two shown) of sloping cylindrical cavities which are interconnected at their upper ends and separated by partitions 13 at their lower ends. Partitions 13 prevent mixing of the sample and reactant liquids when the rotor is at rest while permitting such liquids to pass to sedimentation chambers 14 when the rotor is spinning.
  • An inwardly and downwardly extending passageway 15 leads from the bottom of each sedimentation chamber 14 to the top of a holding chamber 16 disposed below each sedimentation chamber.
  • An upwardly and outwardly extending passageway 17 leads from the base of each holding chamber 16 to the radially innermost part of a cuvette 9.
  • a removable transparent Lucite cover plate 18 is provided to minimize evaporation and prevent windage from deflecting liquids during centrifugal transfer.
  • a conventional centrifuge drive motor 19 supports the rotor assembly 1 while rotating it.
  • a photometer light source and projecting means are provided to project a light beam of constant intensity intersectlng rotor assembly 1 at a point corresponding to the radial positions of cuvettes 9 and spaced holes 10'.
  • the light beam is aligned in such a manner so as to be transmitted through each hole 10 and cuvette 9 as they pass through the beam.
  • the photometric light source comprises an incandescent lamp with a reflecting mirror 21 disposed below the rotor assembly and oriented to reflect the light beam upward, substantially normal to the plane of rotation.
  • Electronic photodetecting means 22 is disposed above rotor assembly 1 and aligned to receive light transmitted through the cuvettes during rotation.
  • Photodetecting means 22 is designed to respond electronically with an output which is proportional to the intensity of the light transmitted from light source 20 through the cuvettes.
  • Photodetector 22 comprises a photomultiplier tube disposed directly above the cuvette circle to receive all light transmitted upwardly through the axially aligned openings.
  • the remaining electronic components illustrated schematically in FIG. 1 include a proportional tachometer 23 which supplies a voltage signal proportional to the rotor speed to a ramp signal generator 24 which, in turn, provides a signal to a pulse scanner 25.
  • a revolution detector 26 synchronizes the ramp signal frequency with the rotor speed.
  • Pulse scanning means 25, synchronizable by the ramp signal generator frequency responds proportionally to pulses originating in photodetecting means 7 22 and sorts the pulses therefrom as to origin. Pulse peak Operation of the invention may best be understood by reference to FIGS. 2 through 6 where a sectional view of transfer dies 11 is shown repeatedly to illustrate successive steps in a typical operation. As shown in FIG.
  • Centrifugal force prevents earlier passage of the supernatant through passageways 15, but as the transfer disc is brought to rest gravitational force predominates to cause the supernatant to flow through the downwardly extending passageways.
  • subsequent transfer of the supernatant from holding chambers 16 is accomplished by further rotation which causes the supernatant to flow outward through passageways 17.
  • the sedimentation chambers 14 shown in the schematic views of FIGS. 1 through 6 are specifically adapted to retain precipitates which form firm adherent pellets upon centrifugation, they may be modified to include a further outwardly extending depression of narrow diameter to contain precipates which do not form such firm adherent pellets.
  • the transfer disc was accelerated and decelerated rapidly to facilitate mixing and then spun for 15 minutes at approximately 6850 rpm.
  • the transfer disc 'Was then stopped to permit drainage of the supernatant into the holding chambers as described in reference to FIG. 5. From each holding chamber a 200 l. sample was withdrawn, diluted with 5 ml. of distilled water and read against distilled water at 260 nm.
  • the principle of operation of the transfer disc described herein is also useful in instances where no precipitate is involved but where two or more solutions are to be mixed together, incubated for a long interval, and subsequently transferred to a cuvett rotor to be read.
  • the transfer disc 11 described herein could also be used in combination with the system for measuring and holding samples described in copending application of common applicant and assignee Ser. No. 806,920.
  • the transfer dies 11 may be used alone or in conjunction with a rotary cuvette system as shown. When used alone, the transfer disc would more properly be referred to as a rotor.
  • the designation transfer disc is used herein inasmuch as its principal use as envisioned by applicant is in conjunction with rotary cuvette systems where it is used to transfer liquids to individual cuvettes. It is intended rather that the invention be limited only by the scope of the appended claims.
  • the improved transfer disc for providing samples free of solid material to said cuvettes comprising:
  • each of said outwardly and upwardly oriented passageways terminates in register with one of said sample analysis cuvettes.
  • a method for photometrically analyzing a multiplicity of discrete samples to simultaneously determine the presence of a single substance therein comprising:

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  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Centrifugal Separators (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
US827185A 1969-05-23 1969-05-23 Multistation analytical photometer and method of use Expired - Lifetime US3586484A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US82718569A 1969-05-23 1969-05-23

Publications (1)

Publication Number Publication Date
US3586484A true US3586484A (en) 1971-06-22

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US827185A Expired - Lifetime US3586484A (en) 1969-05-23 1969-05-23 Multistation analytical photometer and method of use

Country Status (13)

Country Link
US (1) US3586484A (de)
JP (1) JPS4920116B1 (de)
AT (1) AT303424B (de)
BE (1) BE750657A (de)
CH (1) CH519170A (de)
DE (1) DE2022084C3 (de)
ES (1) ES378828A1 (de)
FR (1) FR2043636A1 (de)
GB (1) GB1260804A (de)
IT (1) IT954068B (de)
NL (1) NL7007331A (de)
SE (1) SE367061B (de)
SU (1) SU365081A3 (de)

Cited By (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3771878A (en) * 1970-03-25 1973-11-13 Mse Holdings Ltd Centrifugal photometric analyzer
US3777172A (en) * 1970-10-29 1973-12-04 N Clarke Method and apparatus for measuring the clumping of platelets in whole blood
JPS4946495A (de) * 1972-07-17 1974-05-04
US3810576A (en) * 1969-09-29 1974-05-14 Inventions Dev Corp Ultracentrifuge rotor
US3817632A (en) * 1972-09-21 1974-06-18 Union Carbide Corp Digital subtraction circuit for a centrifugal chemical analyzer of the rotating spectrophotometer type
JPS49104698A (de) * 1973-01-10 1974-10-03
US3943965A (en) * 1973-09-07 1976-03-16 Matelena John J Pipeline for transporting petroleum products through tundra
US3951608A (en) * 1975-01-22 1976-04-20 Ernest Trod Mixing cuvette and timing turntable for providing reaction mixtures
US3953172A (en) * 1974-05-10 1976-04-27 Union Carbide Corporation Method and apparatus for assaying liquid materials
US4055076A (en) * 1975-03-21 1977-10-25 Michele Tropea Centrifugal granulometer
US4090795A (en) * 1977-02-14 1978-05-23 Gesellschaft Fuer Biotechnologische Forschung Mit Beschraenkter Haftung (Gbf) Double sector cell for an ultra-centrifuge
US4148607A (en) * 1975-02-28 1979-04-10 Hoffmann-La Roche Inc. Apparatus and analysis for agglutination reaction
USRE30391E (en) * 1976-02-23 1980-09-02 Abbott Laboratories Chemical analysis cuvette
US4226531A (en) * 1977-08-29 1980-10-07 Instrumentation Laboratory Inc. Disposable multi-cuvette rotor
US4230264A (en) * 1978-02-17 1980-10-28 Akira Okumura Method and apparatus for centrifugal separation of components of solution
US4234799A (en) * 1977-12-16 1980-11-18 Tetsuo Matsumoto Method and instrument for photometric analysis of liquid using centrifuge rotor
US4314970A (en) * 1980-08-27 1982-02-09 Instrumentation Laboratory Inc. Analysis system
US4373812A (en) * 1981-03-25 1983-02-15 Instrumentation Laboratory Inc. Cuvette assembly
US4387164A (en) * 1980-11-05 1983-06-07 Fmc Corporation Method and apparatus for chemical analysis using reactive reagents dispersed in soluble film
US4390499A (en) * 1981-08-13 1983-06-28 International Business Machines Corporation Chemical analysis system including a test package and rotor combination
US4542008A (en) * 1983-10-03 1985-09-17 Olin Corporation Electrochemical chlorine dioxide process
US4557600A (en) * 1981-09-01 1985-12-10 Boehringer Mannheim Gmbh Process and device for the control and mixing of a fluid current subjected to centrifugal force
US4580897A (en) * 1984-05-31 1986-04-08 Allied Corporation Centrifugal analyzer rotors
US4580898A (en) * 1984-05-31 1986-04-08 Allied Corporation Analytical system
US4580896A (en) * 1983-11-07 1986-04-08 Allied Corporation Multicuvette centrifugal analyzer rotor with annular recessed optical window channel
US4680164A (en) * 1985-07-18 1987-07-14 Fisher Scientific Company Centrifugal analyzer
US4902479A (en) * 1983-11-07 1990-02-20 Fisher Scientific Company Centrifugal analyzer rotor
US5071402A (en) * 1986-08-04 1991-12-10 E. I. Du Pont De Nemours And Company Centrifuge rotor having spillage containment groove
US5071625A (en) * 1985-02-27 1991-12-10 Fisher Scientific Company Cuvette handling
US5149501A (en) * 1990-01-29 1992-09-22 Cirrus Diagnostics, Inc. Multichambered container and instrument for performing diagnostic tests
US5199938A (en) * 1990-11-19 1993-04-06 Westfalia Separator Ag Centrifuge drum for concentrating suspended solids
EP0629850A2 (de) * 1989-05-30 1994-12-21 Lockheed Martin Energy Systems, Inc. Rotor und Verfahren zur automatischen Verarbeitung von Flüssigkeiten für labor- und bioanalytische Zwecke
EP0686199A1 (de) * 1993-02-24 1995-12-13 Tetrad Corporation Zentrifugationsverfahren und -vorrichtung zur isolierung einer substanz aus einer mischung
EP0740964A1 (de) * 1995-05-05 1996-11-06 Wells, John Raymond Zentrifuge für automatische Mehrfachdekantierung
US5631166A (en) * 1995-03-21 1997-05-20 Jewell; Charles R. Specimen disk for blood analyses
WO1999025470A1 (en) * 1997-11-19 1999-05-27 Trega Biosciences, Inc. Apparatus and method for separation of liquid and solid phases for solid phase organic syntheses
WO2000044491A3 (en) * 1999-01-29 2000-12-21 Illumina Inc Centrifuging apparatus and method for separation of liquid phases and organic synthesis
US6348176B1 (en) 1999-02-11 2002-02-19 Careside, Inc. Cartridge-based analytical instrument using centrifugal force/pressure for metering/transport of fluids
US20020044894A1 (en) * 1999-12-13 2002-04-18 Michal Lebl Oligonucleotide synthesizer
US6391264B2 (en) 1999-02-11 2002-05-21 Careside, Inc. Cartridge-based analytical instrument with rotor balance and cartridge lock/eject system
US6398972B1 (en) 1999-04-12 2002-06-04 Harvest Technologies Corporation Method for producing platelet rich plasma and/or platelet concentrate
US6531095B2 (en) 1999-02-11 2003-03-11 Careside, Inc. Cartridge-based analytical instrument with optical detector
US6623959B2 (en) 2001-06-13 2003-09-23 Ethicon, Inc. Devices and methods for cell harvesting
US6663832B2 (en) 1999-12-13 2003-12-16 Illumina, Inc. Oligonucleotide synthesizer
US20040208797A1 (en) * 1999-01-29 2004-10-21 Michal Lebl Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses
US20040219063A1 (en) * 2003-01-21 2004-11-04 Heiner David Louis Chemical reaction monitor
USRE38730E1 (en) * 1995-05-05 2005-04-26 Harvest Technologies Corporation Automatic multiple-decanting centrifuge and method of treating physiological fluids
US20050170514A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system for method of standard additions testing
US20050170515A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system with an analytical signal path
US20050169805A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system with a sample chamber
US20050169804A1 (en) * 2004-02-04 2005-08-04 Hach Company User-configurable analytical rotor system
US20050170513A1 (en) * 2004-02-04 2005-08-04 Hach Company Analytical rotor system for titration testing
US20050202733A1 (en) * 2004-03-09 2005-09-15 Brother Kogyo Kabushiki Kaisha Test object receptacle, test apparatus, and test method
US20060094865A1 (en) * 2004-10-29 2006-05-04 Kapur Terri A Intraoperative method for isolating and concentrating autologous growth factors and for forming residual autologous growth factor compositions
US20070087429A1 (en) * 2003-12-08 2007-04-19 Kazuhito Tanimoto Reaction disk and separation cell for automatic analyzer
US20070110638A1 (en) * 2005-09-14 2007-05-17 Heiner David L Continuous polymer synthesizer
US20080207211A1 (en) * 2004-09-15 2008-08-28 Samsung Electronics Co., Ltd. Method and apparatus for indicating preferred layer information in multimedia broadcast/multicast service (MBMS)
US9073033B2 (en) 2010-01-19 2015-07-07 Illumina, Inc. Methods and compositions for processing chemical reactions
US20170095814A1 (en) * 2014-06-16 2017-04-06 Roche Diagnostics Operations, Inc. Cartridge with a rotatable lid

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3813031A (en) * 1972-08-02 1974-05-28 Atomic Energy Commission Rotor having sample holding means
NL7706292A (nl) * 1976-06-09 1977-12-13 Electro Nucleonics Kommenreeks.
EP2214027B1 (de) * 2007-11-20 2019-03-06 Toray Industries, Inc. Analyseverfahren in flüssigkeitszufuhrchip
CN101925820B (zh) * 2008-01-28 2014-02-12 东丽株式会社 分离芯片和分离方法

Cited By (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3810576A (en) * 1969-09-29 1974-05-14 Inventions Dev Corp Ultracentrifuge rotor
US3771878A (en) * 1970-03-25 1973-11-13 Mse Holdings Ltd Centrifugal photometric analyzer
US3777172A (en) * 1970-10-29 1973-12-04 N Clarke Method and apparatus for measuring the clumping of platelets in whole blood
JPS4946495A (de) * 1972-07-17 1974-05-04
JPS5334074B2 (de) * 1972-07-17 1978-09-19
US3817632A (en) * 1972-09-21 1974-06-18 Union Carbide Corp Digital subtraction circuit for a centrifugal chemical analyzer of the rotating spectrophotometer type
JPS49104698A (de) * 1973-01-10 1974-10-03
US3943965A (en) * 1973-09-07 1976-03-16 Matelena John J Pipeline for transporting petroleum products through tundra
US3953172A (en) * 1974-05-10 1976-04-27 Union Carbide Corporation Method and apparatus for assaying liquid materials
US3951608A (en) * 1975-01-22 1976-04-20 Ernest Trod Mixing cuvette and timing turntable for providing reaction mixtures
US4148607A (en) * 1975-02-28 1979-04-10 Hoffmann-La Roche Inc. Apparatus and analysis for agglutination reaction
US4055076A (en) * 1975-03-21 1977-10-25 Michele Tropea Centrifugal granulometer
USRE30391E (en) * 1976-02-23 1980-09-02 Abbott Laboratories Chemical analysis cuvette
US4090795A (en) * 1977-02-14 1978-05-23 Gesellschaft Fuer Biotechnologische Forschung Mit Beschraenkter Haftung (Gbf) Double sector cell for an ultra-centrifuge
US4226531A (en) * 1977-08-29 1980-10-07 Instrumentation Laboratory Inc. Disposable multi-cuvette rotor
US4234799A (en) * 1977-12-16 1980-11-18 Tetsuo Matsumoto Method and instrument for photometric analysis of liquid using centrifuge rotor
US4230264A (en) * 1978-02-17 1980-10-28 Akira Okumura Method and apparatus for centrifugal separation of components of solution
US4314970A (en) * 1980-08-27 1982-02-09 Instrumentation Laboratory Inc. Analysis system
US4387164A (en) * 1980-11-05 1983-06-07 Fmc Corporation Method and apparatus for chemical analysis using reactive reagents dispersed in soluble film
US4373812A (en) * 1981-03-25 1983-02-15 Instrumentation Laboratory Inc. Cuvette assembly
US4390499A (en) * 1981-08-13 1983-06-28 International Business Machines Corporation Chemical analysis system including a test package and rotor combination
US4557600A (en) * 1981-09-01 1985-12-10 Boehringer Mannheim Gmbh Process and device for the control and mixing of a fluid current subjected to centrifugal force
US4542008A (en) * 1983-10-03 1985-09-17 Olin Corporation Electrochemical chlorine dioxide process
US4580896A (en) * 1983-11-07 1986-04-08 Allied Corporation Multicuvette centrifugal analyzer rotor with annular recessed optical window channel
US4902479A (en) * 1983-11-07 1990-02-20 Fisher Scientific Company Centrifugal analyzer rotor
US4580897A (en) * 1984-05-31 1986-04-08 Allied Corporation Centrifugal analyzer rotors
US4580898A (en) * 1984-05-31 1986-04-08 Allied Corporation Analytical system
US5071625A (en) * 1985-02-27 1991-12-10 Fisher Scientific Company Cuvette handling
US4680164A (en) * 1985-07-18 1987-07-14 Fisher Scientific Company Centrifugal analyzer
US5071402A (en) * 1986-08-04 1991-12-10 E. I. Du Pont De Nemours And Company Centrifuge rotor having spillage containment groove
EP0629850A2 (de) * 1989-05-30 1994-12-21 Lockheed Martin Energy Systems, Inc. Rotor und Verfahren zur automatischen Verarbeitung von Flüssigkeiten für labor- und bioanalytische Zwecke
EP0629850A3 (en) * 1989-05-30 1994-12-28 Martin Marietta Energy Systems Rotor and method for automatically processing liquids for laboratory and bioanalysis purposes.
US5149501A (en) * 1990-01-29 1992-09-22 Cirrus Diagnostics, Inc. Multichambered container and instrument for performing diagnostic tests
US5199938A (en) * 1990-11-19 1993-04-06 Westfalia Separator Ag Centrifuge drum for concentrating suspended solids
US5610074A (en) * 1993-02-24 1997-03-11 Beritashvili; David R. Centrifugal method and apparatus for isolating a substance from a mixture of substances in a sample liquid
EP0686199A4 (de) * 1993-02-24 1996-06-26 Tetrad Corp Zentrifugationsverfahren und -vorrichtung zur isolierung einer substanz aus einer mischung
EP0686199A1 (de) * 1993-02-24 1995-12-13 Tetrad Corporation Zentrifugationsverfahren und -vorrichtung zur isolierung einer substanz aus einer mischung
US5631166A (en) * 1995-03-21 1997-05-20 Jewell; Charles R. Specimen disk for blood analyses
US5885528A (en) * 1995-03-21 1999-03-23 Immutech, Inc. Apparatus for blood analysis
US6024883A (en) * 1995-03-21 2000-02-15 Immutech, Inc. Method for blood analyses
EP0740964A1 (de) * 1995-05-05 1996-11-06 Wells, John Raymond Zentrifuge für automatische Mehrfachdekantierung
US5707331A (en) * 1995-05-05 1998-01-13 John R. Wells Automatic multiple-decanting centrifuge
USRE38730E1 (en) * 1995-05-05 2005-04-26 Harvest Technologies Corporation Automatic multiple-decanting centrifuge and method of treating physiological fluids
USRE38757E1 (en) * 1995-05-05 2005-07-12 Harvest Technologies Corporation Automatic multiple-decanting centrifuge and container therefor
WO1999025470A1 (en) * 1997-11-19 1999-05-27 Trega Biosciences, Inc. Apparatus and method for separation of liquid and solid phases for solid phase organic syntheses
US6121054A (en) * 1997-11-19 2000-09-19 Trega Biosciences, Inc. Method for separation of liquid and solid phases for solid phase organic syntheses
US7977456B2 (en) 1999-01-29 2011-07-12 Illumina, Inc. Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses
US8178652B2 (en) 1999-01-29 2012-05-15 Illumina, Inc. Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses
US6846460B1 (en) 1999-01-29 2005-01-25 Illumina, Inc. Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses
WO2000044491A3 (en) * 1999-01-29 2000-12-21 Illumina Inc Centrifuging apparatus and method for separation of liquid phases and organic synthesis
US20040208797A1 (en) * 1999-01-29 2004-10-21 Michal Lebl Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses
US8394923B2 (en) 1999-01-29 2013-03-12 Illumina, Inc. Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses
US6531095B2 (en) 1999-02-11 2003-03-11 Careside, Inc. Cartridge-based analytical instrument with optical detector
US6391264B2 (en) 1999-02-11 2002-05-21 Careside, Inc. Cartridge-based analytical instrument with rotor balance and cartridge lock/eject system
US6348176B1 (en) 1999-02-11 2002-02-19 Careside, Inc. Cartridge-based analytical instrument using centrifugal force/pressure for metering/transport of fluids
US6398972B1 (en) 1999-04-12 2002-06-04 Harvest Technologies Corporation Method for producing platelet rich plasma and/or platelet concentrate
US6663832B2 (en) 1999-12-13 2003-12-16 Illumina, Inc. Oligonucleotide synthesizer
US20110143965A1 (en) * 1999-12-13 2011-06-16 Michal Lebl Oligonucleotide synthesizer
US20020044894A1 (en) * 1999-12-13 2002-04-18 Michal Lebl Oligonucleotide synthesizer
US8465694B2 (en) 1999-12-13 2013-06-18 Illumina, Inc. Oligonucleotide synthesizer
US20090023605A1 (en) * 1999-12-13 2009-01-22 Michal Lebl Oligonucleotide synthesizer
US7390459B2 (en) 1999-12-13 2008-06-24 Illumina, Inc. Oligonucleotide synthesizer
US6623959B2 (en) 2001-06-13 2003-09-23 Ethicon, Inc. Devices and methods for cell harvesting
US20040219063A1 (en) * 2003-01-21 2004-11-04 Heiner David Louis Chemical reaction monitor
US20110136684A1 (en) * 2003-01-21 2011-06-09 David Louis Heiner Chemical reaction monitor
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Also Published As

Publication number Publication date
BE750657A (fr) 1970-11-03
JPS4920116B1 (de) 1974-05-22
SE367061B (de) 1974-05-13
AT303424B (de) 1972-11-27
DE2022084C3 (de) 1979-11-15
SU365081A3 (de) 1972-12-28
ES378828A1 (es) 1972-07-16
IT954068B (it) 1973-08-30
DE2022084A1 (de) 1970-11-26
CH519170A (de) 1972-02-15
NL7007331A (de) 1970-11-25
GB1260804A (en) 1972-01-19
FR2043636A1 (de) 1971-02-19
DE2022084B2 (de) 1979-03-08

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