Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
  • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
  • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives

Definitions

• the present invention relates to reagent and reagent mixtures useful for detecting and measuring alkaline and acid phosphatases in biological liquids, such as serum, and to methods of assaying biological liquids using these reagent mixtures.
• the disodium salt of para-nitrophenyl phosphoric acid is known in the art as a substrate for this enzymatic reaction.
• its stability in dry form is limited, and it is also more difiicult to prepare than the here claimed novel salts.
• the stable reagent is an alkyl amine salt of paranitrophenyl phosphoric acid.
• This salt is very stable and can be made available as unit amounts in foil containers or capsules. Each of these units w ill contain just a sufficient quantity of the assay material for making a single assay of a specimen.
• Suitable buffers and metal activators may be added to the reagent to give a stable reagent mixture.
• a package containing the assay material for making the particular assay is selected.
• the assay material contained in the package is pre-measured and of a predetermined activity.
• liquid reagent may be dissolved directly in a standard amount of water so as to form a liquid reagent.
• This liquid reagent is then mixed with the biological specimen to produce an enzymatic reaction.
• the extent of or the rate at which the reaction occurs will be a function of the quantity of amount of activity of the original unknown.
• the assay material is dissolved to formfa liquid reagent and the reagent is mixed with the specimen, the substrate will react with the "unknown.
• the enzyme in order for the reaction to occur successfully, it isf'necessary for the enzyme to catalyze the reaction.
• the quantity of the substrate contained in the reagent is in excess of that required to cause all of the unknown to completely react or to react at a desired rate. As a result the only factor that limits the assay reaction will be the quantity or amount of activity of the unknown.
• the substrates When the substrates are in a pure solid dry form, they may be ground into a dry powder suitable for mixing with the lyophilized powder.
• the substrate enters into the reaction and is converted from one form to another form.
• the extent to which the substrate is converted is determined by the extent to which the assay reaction progresses.
• the substrate may be readily converted from one form (p-nitrophenyl phosphoric acid) to another form (p-nitrophenyl).
• the substrate has a light absorption at some particular wavelength only when it is in the converted form. When it is in the other form, it is transparent at the designated wavelength, although the absorption band may be any desired wavelength that is convenient to use. However, it is desirable that it be distinct from the intense absorption bands of the rest of the components in the assay material and the substances in the specimen.
• the amount of the substrate converted may be determined. More specifically, by measuring the amount of change or rate of change of the optical density at the designated wavelength, the amount or rate of the assay reaction may be measured. It has been found that p-NPP is transparent at 415 millimicrons. When the converted form (p-nitrophenol) shows absorption of ultraviolet light with a maximum value at a wavelength of about 415 millimicrons in alkaline solution. By employing this substrate, the assay reaction may be observed by always measuring the optical density at this wavelength.
• Free para nitrophenyl phosphoric acid may be obtained by any standard procedure, such as the passage of an alkali metal salt of p-NPP thru a cationic exchange resin, for example, Dowex 50 resin, in the acid form.
• a cationic exchange resin for example, Dowex 50 resin
• the amine salts of para-nitrophenyl phosphoric acids may also be obtained by reacting an appropriate amine with an alkali metal salt of para nitrophenyl phosphoric acid. In this manner, the amine salt of para nitrophenyl phosphoric acid may be precipitated by concentration, or by the addition of an organic solvent.
• the free para nitrophenyl phosphoric acid is neutralized with the appropriate amine in solution, and precipitated by the addition of an organic solvent, or evaporated until crystallization sets in.
• the corresponding para nitrophenyl phosphoric acid amine salt is washed, collected, and dried.
• the particular amine salts employed will depend upon their availability and their ability to stabilize p-NPPA under test conditions.
• the salts will normally be chosen from a class that includes aliphatic amines, alicyclic alkylamines, hydroxyalkyl amines, arylamines, and aralkylamines.
• alkyl amines are monoalkyl, dialkyl and trialkyl, with from 1 to 18, preferably 1-6, carbon atoms in the alkyl groups.
• Specific examples are methylamine, ethylamine, tributylamine, and dipropylamine.
• alicylic alkyl amines are monocyclohexylamine, dicyclohexylamine, and tricyclohexylamine.
• hydroxylalkyl amines are those with from 2 to 12, preferably 26, carbon atoms in said alkyl groups, exemplary thereof are monoethanolamine, and Z-amino- Z-hydroxymethyL1,3-propane diol.
• aralkyl amines with 5 to 15, preferably 7-9, carbon atoms, are exemplified by monobenzyl amine.
• EXAMPLE 1 Preparation of diethanolamine salt of p-nitrophenyl phosphate A solution of 12.5 gms. of disodiurn para nitrophenyl phosphate of high purity (such as obtained from Calbiochem) in 50 ml. of water, is passed thru a column of ml. of Dowex 50 resin, 50-100 mesh, hydrogen ion form. The column is washer with 200 ml. of water. To the column efiluent is added 6 gms. of ethanolarnine. The solution was evaporated under reduced pressure to approximately 25 ml. and 600 ml. of acetone added. The mixture was then cooled to 0 C. for 2 hours and the precipitate collected on a filter and washed with acetone. The product was dried in vacuum to constant weight. Yield: 7.0 gms. of p-nitrophenyl phosphoric acid, didiethanolamine salt.
• EXAMPLE 2 Preparation of dimorpholine salt of p-nitrophenylphosphate
• a solution of 12.5 gms. of disodium para nitrophenyl phosphate of high purity (from Calbiochem) in 50 ml. of Water is passed thnu a column of 125 ml. of a cationic exchange resin such as Dowex 50 resin, 50-100 mesh, hydrogen ion form. The column is washed with 200 ml. of water. To the column of afiiuent is added 13 gms. of morpholine. The solution is then evaporated under reduced pressure to a volume of approximately 25 ml., and 700 ml. of acetone added. The precipitant was collected, washed with cold acetone, and dried in vacuum to constant weight. Yield: 7.75 gms. of p-nitrop'henyl phosphoric acid, dimorpholine salt.
• EXAMPLE 3 Preparation of di(cyclohexylammonium-p-nitrophenyl phosphate A solution of 12.5 gms. of disodium para nitrophenyl phosphate of high purity (from Calbiochem) in 50 ml. of Water, was passed thru a column of 125 ml. of a cationic exchange resin, such as Dowex 50 resin, 50-100 mesh, hydrogen ion form. The column was washed with 200 ml. of water. To the column efiiuent is added 10 gms. of cyclohexylamine. The solution is evaporated under reduced pressure to approximately 25 ml., and 400 ml. of acetone is added.
• a cationic exchange resin such as Dowex 50 resin, 50-100 mesh
• amino salts of p-NPP prepared as described above, are next tested to determine their suitability as substrates in an assay for alkaline phosphatase activity, by being compared to a standard salt form, such as disodium pnitrophenyl phosphoric acid. This is measured by determination of rate of hydrolysis and the percent of free p-nitrophenol formed from equimolar aqueous solutions of the listed amine salts.
• a dry reagent powder contains p-nitrophenyl phosphoric in stabilized form as one of its amine salts (as an example).
• a buffer from the class that includes alkali metal salts of carbonates, such as disodium and sodium hydrogen carbonates.
• an metal activator such as a magnesium salt is included, preferably magnesium aspartate.
• the pH is maintained within the broad range of between 4 and 11, by appropriate selection of one or more of the buffers well known in the art for this purpose.
• this powder As long as this powder is maintained dry, it is very stable and will have a long shelf life. Accordingly, it may then be divided into unit amounts to form a liquid reagent with water suitable for making a single assay of a serum.
• Each of these parts may then be pack-aged into a suitable container such as a capsule for subsequent use.
• an aqueous solution of the amine may be frozen and lyophilized, without as great a formation of p nitrophenol as in the case of the sodium salt.
• a specimen of the biological fluid such 2.
• the method of assaying a specimen for the enzymes, as serum is first obtained.
• the assay acid or alkaline phosphatases, including the steps of material in one of the capsules of this example is disdissolving into water, a substantially anhydrous solid solved in a suitable quantity of water.
• a reagent material comprising: a liquid reagent having the right size for making a single the substrate para-nitrophenyl phosphoric acid amine assay of the serum.
• This liquid reagent is thus mixed salt; with the specimen.
• the following reaction will 10 uniform rate of reaction catalyzed by the enzyme occur: being determined, thereby to produce a liquid reagent alkaline phosphatase Amme salt of p-NN P W n1trophenol+salt of phosphoric acid This reaction is dependent upon being catalyzed by the having a measurable optical density; enzyme present in the serum.
• mixing said liquid with said specimen to form a speci- What is claimed is: men-reagent assay mixture, and 1.
• a stable assay material for dissolving in water to measuring the rate of change of optical density of the create a liquid reagent for assaying a specimen for the reacting specimen-assay mixture enzymes, alkaline or acid phosphatases, including the combination of; References the substrate para-nitrophenyl phosphoric acid amine UNITED STATES PATENTS salt; a dry bulfer capable of maintaining the pH between 2359052 9/1944 Scharer 195 1035 4 and ALVIN E. TANENHOLTZ, Primary Examiner. a metal activator comprising a magnesium salt; each substance above being present in that quantity so as to insure a uniform rate of reaction catalyzed 260924

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• 1966
  • 1966-11-14 US US593740A patent/US3425912A/en not_active Expired - Lifetime
• 1967
  • 1967-10-29 IL IL28842A patent/IL28842A/xx unknown
  • 1967-11-01 GB GB35174/69A patent/GB1211722A/en not_active Expired
  • 1967-11-01 GB GB49698/67A patent/GB1211721A/en not_active Expired
  • 1967-11-07 DK DK554067AA patent/DK140267B/da not_active IP Right Cessation
  • 1967-11-10 SE SE15439/67A patent/SE336687B/xx unknown
  • 1967-11-10 NL NL6715288A patent/NL6715288A/xx unknown
  • 1967-11-10 SE SE7006732A patent/SE375384B/xx unknown
  • 1967-11-13 CA CA004851A patent/CA930655A/en not_active Expired
  • 1967-11-13 DE DE1673047A patent/DE1673047C2/de not_active Expired
  • 1967-11-14 CH CH1587567A patent/CH497702A/de not_active IP Right Cessation
  • 1967-11-14 SU SU1197398A patent/SU444376A3/ru active

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