US20180016540A1 - Incubator - Google Patents
Incubator Download PDFInfo
- Publication number
- US20180016540A1 US20180016540A1 US15/719,323 US201715719323A US2018016540A1 US 20180016540 A1 US20180016540 A1 US 20180016540A1 US 201715719323 A US201715719323 A US 201715719323A US 2018016540 A1 US2018016540 A1 US 2018016540A1
- Authority
- US
- United States
- Prior art keywords
- electric power
- power supplied
- heating
- magnitude
- incubator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Definitions
- the present disclosure relates to an incubator.
- incubators for cultivation of cells and microorganisms and so on, in incubation chambers have been known.
- Such an incubator comprises a heater for heating the inside of the incubation chamber where a humidification pan is placed, and, by controlling the heater, the inside of the incubation chamber may be maintained at a predetermined temperature (37 degrees C., for example) and also at predetermined humidity (95% RH, for example) based on the predetermined temperature, for example.
- an incubator that includes a heating element for heating water stored in a recess formed on the bottom face, a heating element for heating the inside of the incubation chamber except for the recess, and a heating element provided on an insulated door, which is openably and closably attached to the insulated box body, and, by controlling electric power supplied to the three heating elements so as to maintain the temperature of the water in the recess lower than the temperature in the incubation chamber, supersaturated content of water within the incubation chamber is returned to the recess, thereby preventing dew condensation (see Patent Document 1).
- This incubator also comprises a temperature detection device for detecting a temperature within the incubation chamber and is configured to control each of the multiple heating elements based on the detection result of the temperature detection device.
- Patent Document 1 Japanese Unexamined Patent Application Publication No. 5-227942
- temperatures within the incubation chamber are not necessarily uniform. Accordingly, even when the temperature near the temperature detection device stands at a desired value, the temperature of a partial region in the incubation chamber may be relatively lower than the temperature therearound. In this case, dew condensation may be caused on the wall surface in the region of which the temperature is relatively lower than the temperature therearound. The dew condensation may cause the problem of growth of unwanted bacteria in the condensed water, which negatively affects the cultures.
- the present invention is made in view of such a situation, and a purpose thereof is to provide a new technique for preventing dew condensation in an incubator.
- an incubator of an embodiment of the present invention includes: an insulated box including an incubation space surrounded by a plurality of inner faces; a humidifying unit that humidifies an incubation space; a plurality of heating units that respectively heat the plurality of inner faces, with electric power supplied; and a control unit that controls the magnitude of electric power supplied to each of the plurality of heating units.
- the control unit periodically and repeatedly changes the magnitude of electric power supplied to the plurality of heating units, at timing different for each of the plurality of inner faces.
- FIG. 1 is a perspective view that shows a schematic configuration of an incubator according to an embodiment
- FIG. 2 is a sectional view of the incubator according to the embodiment
- FIG. 3 is a schematic diagram for describing heating units in the incubator
- FIG. 4 is a block diagram that shows a configuration of a control unit that controls the heating units
- FIG. 5 is a timing chart that shows variation of the magnitude of electric power supplied to each of the heating units according to a first embodiment
- FIG. 6 is a timing chart that shows variation of the magnitude of electric power supplied to each of the heating units according to a second embodiment
- FIG. 7 is a timing chart that shows variation of the magnitude of electric power supplied to each of the heating units according to a third embodiment
- FIG. 8 is a timing chart that shows variation of the magnitude of electric power supplied to each of the heating units according to a fourth embodiment.
- FIG. 9 is a timing chart that shows variation of the magnitude of electric power supplied to each of the heating units according to a fifth embodiment.
- FIG. 1 is a perspective view that shows a schematic configuration of an incubator according to an embodiment.
- FIG. 1 shows the state where an insulated door of the incubator is open.
- FIG. 2 is a sectional view of the incubator according to the embodiment.
- an incubator 1 comprises a left opening door (more specifically, an outer door and an inner door) and small doors, which are hinged double doors.
- a space surrounded by an insulated box body 2 which includes an opening 2 A on the front side, and a transparent door 3 , which is an inner door openably and closably covering the opening 2 A, is formed as an incubation chamber 4 .
- the transparent door 3 is openably and closably hinged to the insulated box body 2 on the left side and hermetically covers the opening 2 A with a gasket 2 B provided as a seal member on the frontage of the incubation chamber 4 .
- the interior of the incubation chamber 4 is horizontally partitioned by multiple shelves 5 (partitioned into five by four shelves in this example).
- the incubator 1 is used as a CO 2 incubator, for example, the CO 2 concentration is often set and maintained at about 5%, so that, after the door is closed, CO 2 gas is supplied into the incubation chamber 4 for CO 2 concentration control.
- multiple pairs of small doors 6 A and 6 B which are hinged double doors, are provided for the respective sections (five pairs in this example) inside the transparent door 3 so that outside air is not introduced into the whole incubation chamber 4 partitioned into multiple sections even when the transparent door 3 is opened.
- An insulated door 7 is openably and closably hinged to the insulated box body 2 and functions as an outer door to prevent heat from entering through the opening 2 A of the incubation chamber 4 , and a gasket 8 containing a magnet is provided along the circumference on the back side of the insulated door 7 .
- a duct 11 On the back face and the bottom face of the incubation chamber 4 is provided a duct 11 , which consists of a back duct 11 A and a bottom duct 11 B that each include a space for forming a gas passage K.
- forced gas circulation is performed, in which gas within the incubation chamber 4 is suctioned through a suctioning port 12 , formed above the back duct 11 A, and then expelled through an expulsion port 13 , provided on the right and left sides and the front side of the bottom duct 11 B, into the incubation chamber 4 .
- a circulation blower 14 is provided for the forced gas circulation.
- the circulation blower 14 comprises a fan, a motor, and a shaft; the motor is disposed in a machine chamber 19 provided outside and on the back face of the incubation chamber 4 , as described later, and the shaft extends from the motor in the machine chamber 19 to the gas passage K, piercing the back face of the insulated box body 2 , to be connected to the fan.
- a humidification pan 15 for storing water for humidification (i.e., humidifying water) 16 is placed and heated by a heater provided outside and on the bottom face of an inner box 22 made of metal, such as stainless steel, so that the water is evaporated.
- a heater provided outside and on the bottom face of an inner box 22 made of metal, such as stainless steel.
- the machine chamber 19 On the back face of an outer box 21 of the insulated box body 2 , the machine chamber 19 is provided in which the motor as a driving means of the circulation blower 14 , a gas supplying means 17 for supplying CO 2 gas to the incubation chamber 4 , and electrical components, such as a control substrate, not illustrated, are disposed.
- the gas supplying means 17 comprises a gas supply pipe 17 A, an on-off valve 17 B, and a filter 17 C, and the tip of the gas supply pipe 17 A is positioned within the gas passage K.
- a jet of CO 2 gas supplied through the gas supply pipe 17 A can be provided.
- the insulated box body 2 comprises the outer box 21 made of metal, the inner box 22 made of stainless steel, an insulating material 24 provided between the outer box 21 and the inner box 22 and on the inner surfaces of the outer box 21 , and an air layer (so-called an air jacket) 25 provided inside the insulating material 24 .
- an air layer (so-called an air jacket) 25 provided inside the insulating material 24 .
- heaters for heating the incubation chamber are provided as heating units (which will be described later). Heaters may also be provided on the transparent door 3 and the insulated door 7 covering the opening 2 A of the incubation chamber 4 .
- FIG. 3 is a schematic diagram for describing heating units in the incubator. Illustrations of members unnecessary for the description are appropriately omitted.
- the incubation chamber 4 in the aforementioned incubator 1 includes an incubation space of a rectangular parallelepiped shape surrounded by six inner faces. In the incubator 1 , six heaters 26 A- 26 F are provided as heating units for heating the respective six inner faces.
- the heater 26 A is pasted over the outer surface of the inner face of the insulated door 7 .
- the heater 26 B is pasted over the outer surface of the top board.
- the heater 26 C is pasted over the outer surface of the right side.
- the heater 26 D is pasted over the outer surface of the bottom face.
- the heater 26 E is pasted over the outer surface of the left side.
- the heater 26 F is pasted over the outer surface of the back face.
- FIG. 4 is a block diagram that shows a configuration of a control unit that controls the heating units.
- FIG. 5 is a timing chart that shows variation of the magnitude of electric power supplied to each of the heating units according to the first embodiment.
- the incubator 1 of the first embodiment comprises: the insulated box body 2 including the incubation chamber 4 as an incubation space surrounded by multiple inner faces; the humidification pan 15 as a humidifying unit that humidifies the inside of the incubation chamber 4 ; the heaters 26 A- 26 F that heat the respective multiple inner faces of the incubation chamber 4 with electric power supplied; a power supply unit 28 that supplies electric power; and a control unit 30 that controls the magnitude of electric power supplied to each of the heaters.
- the control unit 30 repeatedly changes the magnitude of electric power supplied to the heater 26 A at timing t 1 (period T), repeatedly changes the magnitude of electric power supplied to the heater 26 B at timing t 2 (period T), repeatedly changes the magnitude of electric power supplied to the heater 26 C at timing t 3 (period T), repeatedly changes the magnitude of electric power supplied to the heater 26 D at timing t 4 (period T), repeatedly changes the magnitude of electric power supplied to the heater 26 E at timing t 5 (period T), and repeatedly changes the magnitude of electric power supplied to the heater 26 F at timing t 6 (period T).
- the timing t 1 -t 6 is different from each other.
- the period T of the repetitive change of electric power may not necessarily be the same for all the heaters.
- a period T′ of part of the heaters may be made different from the period T of the other heaters.
- the period T of a heater may not necessarily be always the same. For example, after electric power is changed with a period T 1 , it may be changed with a period T 2 (T 2 ⁇ T 1 ) and then changed with the period T 1 again.
- changing the magnitude of electric power means changing the state between that where electric power is not supplied (OFF) and that where a predetermined amount of electric power is supplied (ON); however, it is not limited to such changing. For example, it may be changing the state between that where a first amount (greater than zero) of electric power is supplied and that where a second amount, greater than the first amount, of electric power is supplied.
- the temperatures of the multiple inner faces forming the incubation chamber 4 are not necessarily completely uniform. Accordingly, if there is a part having a relatively low temperature in a certain region within the multiple inner faces for a long time, dew condensation may be caused in the region.
- the state of heating by each heater can be repeatedly changed at different timing on each of the multiple inner faces of the incubation chamber 4 forming an incubation space, thereby preventing the situation where a part having a relatively low temperature continuously exists in a certain region for a long time. Consequently, dew condensation can be prevented.
- FIG. 6 is a timing chart that shows variation of the magnitude of electric power supplied to each of the heating units according to a second embodiment.
- the control unit 30 repeatedly changes the magnitude of electric power supplied to the heaters 26 A and 26 F at timing t 1 (period T), repeatedly changes the magnitude of electric power supplied to the heaters 26 B and 26 D at timing t 2 (period T), and repeatedly changes the magnitude of electric power supplied to the heaters 26 C and 26 E at timing t 3 (period T).
- the multiple heaters may be configured as a single heater.
- the heaters 26 B and 26 D, or the heaters 26 C and 26 E may be configured as one heater.
- FIG. 7 is a timing chart that shows variation of the magnitude of electric power supplied to each of the heating units according to a third embodiment.
- the control unit 30 repeatedly changes the magnitude of electric power supplied to the heaters 26 A, 26 D, and 26 F at timing tl (period T), and repeatedly changes the magnitude of electric power supplied to the heaters 26 B, 26 C, and 26 E at timing t 2 (period T).
- the multiple heaters may be configured as a single heater.
- the heaters 26 D and 26 F, or the heaters 26 B, 26 C, and 26 E may be configured as one heater.
- FIG. 8 is a timing chart that shows variation of the magnitude of electric power supplied to each of the heating units according to a fourth embodiment.
- the control unit 30 repeatedly changes the magnitude of electric power supplied to the heater 26 A at timing tl (period T), repeatedly changes the magnitude of electric power supplied to the heater 26 B at timing t 2 (period T), repeatedly changes the magnitude of electric power supplied to the heater 26 C at timing t 3 (period T), repeatedly changes the magnitude of electric power supplied to the heater 26 D at timing t 41 -t 43 (period T 2 ), repeatedly changes the magnitude of electric power supplied to the heater 26 E at timing t 5 (period T), and repeatedly changes the magnitude of electric power supplied to the heater 26 F at timing t 6 (period T).
- control unit provides control so that electric power supplied to the heater 26 D is greater than electric power supplied to each of the other heaters. This prevents dew condensation near the bottom face, where a temperature drop is more likely to occur because of heat consumption due to vaporization of water in the humidification pan 15 and a natural temperature gradient, by heating the bottom face more than the other inner faces.
- FIG. 9 is a timing chart that shows variation of the magnitude of electric power supplied to each of the heating units according to a fifth embodiment.
- the control unit 30 provides control so that electric power supplied to the heater 26 B is less than electric power supplied to each of the other heaters. Accordingly, heating near the top board, where the temperature tends to rise, can be restrained, so that the temperatures within the incubation space can be made more uniform.
- the incubator set forth above can also be expressed as follows. Namely, the incubator 1 comprises: the insulated box body 2 including the incubation chamber 4 surrounded by multiple inner faces; the humidification pan 15 that humidifies the incubation chamber 4 ; the multiple heaters 26 A- 26 F that heat the multiple inner faces; and the control unit 30 that controls the magnitude of electric power supplied to each of the multiple heaters 26 A- 26 F.
- the control unit 30 controls electric power supplied to each of the multiple heaters 26 A- 26 F so as to repeatedly change the temperature distribution on the multiple inner faces at predetermined timing.
- the incubator 1 repeatedly changes the temperature distribution on the multiple inner faces forming the incubation chamber 4 at predetermined timing, thereby preventing the situation where a part having a relatively low temperature continuously exists in a certain region for a long time. Consequently, dew condensation can be prevented.
- the incubator 1 may be an incubator connected to another device, such as an isolator, or may be configured as a system including an isolator.
- the incubator 1 includes the heaters 26 A- 26 F for heating the respective six inner faces surrounding the incubation space as heating units controlled to be repeatedly changed at predetermined timing
- the number and the arrangement of the heaters are not limited to those described in the embodiments.
- the number of the heaters may be at least two, and, with regard to the arrangement of the heaters, the six faces may not necessarily be sectioned for each inner face; for example, the inner box 22 may be sectioned vertically or horizontally, and two heaters for heating the respective sectioned regions may be provided.
- the inner box 22 may be sectioned into regions that include the respective corners. In this way, the multiple inner faces surrounding the incubation space may be sectioned into at least two regions, and the heating units controlled to be repeatedly changed at predetermined timing may be provided to heat the respective regions.
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16/352,329 US10704020B2 (en) | 2015-03-30 | 2019-03-13 | Incubator |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015069862 | 2015-03-30 | ||
JP2015-069862 | 2015-03-30 | ||
PCT/JP2016/057908 WO2016158337A1 (ja) | 2015-03-30 | 2016-03-14 | 培養装置 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2016/057908 Continuation WO2016158337A1 (ja) | 2015-03-30 | 2016-03-14 | 培養装置 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US16/352,329 Continuation US10704020B2 (en) | 2015-03-30 | 2019-03-13 | Incubator |
Publications (1)
Publication Number | Publication Date |
---|---|
US20180016540A1 true US20180016540A1 (en) | 2018-01-18 |
Family
ID=57006701
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US15/719,323 Abandoned US20180016540A1 (en) | 2015-03-30 | 2017-09-28 | Incubator |
US16/352,329 Active 2036-03-17 US10704020B2 (en) | 2015-03-30 | 2019-03-13 | Incubator |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US16/352,329 Active 2036-03-17 US10704020B2 (en) | 2015-03-30 | 2019-03-13 | Incubator |
Country Status (5)
Country | Link |
---|---|
US (2) | US20180016540A1 (zh) |
EP (1) | EP3260529B1 (zh) |
JP (1) | JP6386164B2 (zh) |
CN (1) | CN107406812B (zh) |
WO (1) | WO2016158337A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11959060B1 (en) | 2019-05-17 | 2024-04-16 | Humacyte, Inc. | Fluid systems, apparatuses, devices and methods of management thereof for cultivating tissue |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6616886B2 (ja) | 2016-03-28 | 2019-12-04 | Phcホールディングス株式会社 | 培養装置 |
JP6473552B2 (ja) * | 2016-11-10 | 2019-02-20 | 株式会社片岡製作所 | 培養容器収容装置 |
WO2023068061A1 (ja) * | 2021-10-22 | 2023-04-27 | Phcホールディングス株式会社 | 培養装置 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
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JPS474296U (zh) * | 1971-02-05 | 1972-09-09 | ||
JPH05227942A (ja) * | 1992-02-25 | 1993-09-07 | Unie Data:Kk | 培養装置 |
JP3197696B2 (ja) | 1993-07-22 | 2001-08-13 | 三洋電機株式会社 | 培養装置 |
DE4441250C1 (de) * | 1994-11-19 | 1996-04-25 | Binder Peter Michael | Brutschrank |
KR101090570B1 (ko) * | 2002-12-09 | 2011-12-08 | 산요덴키 바이오메디칼 가부시키가이샤 | 인큐베이터 |
EP2031051B1 (en) * | 2006-06-16 | 2021-05-19 | PHC Holdings Corporation | Culture monitoring system |
JP5011488B2 (ja) * | 2008-08-27 | 2012-08-29 | パナソニックヘルスケア株式会社 | 培養装置 |
CN102478340B (zh) * | 2010-11-26 | 2015-04-08 | 泰州乐金电子冷机有限公司 | 防结露加热装置及其方法 |
JP5897855B2 (ja) * | 2011-09-22 | 2016-04-06 | パナソニックヘルスケアホールディングス株式会社 | ヒーターを備えた培養装置 |
CN103672399A (zh) * | 2013-12-19 | 2014-03-26 | 常熟市加腾电子设备厂(普通合伙) | 防结露氮气柜 |
CN104932582B (zh) * | 2014-04-01 | 2017-03-15 | 中国建筑材料科学研究总院 | 一种防止玻璃结露结霜的控制方法 |
CN103994506A (zh) * | 2014-04-29 | 2014-08-20 | 太仓艺斯高医疗器械科技有限公司 | 一种恒温恒湿装置及其调节方法 |
-
2016
- 2016-03-14 CN CN201680016109.1A patent/CN107406812B/zh active Active
- 2016-03-14 EP EP16772220.6A patent/EP3260529B1/en active Active
- 2016-03-14 JP JP2017509500A patent/JP6386164B2/ja active Active
- 2016-03-14 WO PCT/JP2016/057908 patent/WO2016158337A1/ja active Application Filing
-
2017
- 2017-09-28 US US15/719,323 patent/US20180016540A1/en not_active Abandoned
-
2019
- 2019-03-13 US US16/352,329 patent/US10704020B2/en active Active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11959060B1 (en) | 2019-05-17 | 2024-04-16 | Humacyte, Inc. | Fluid systems, apparatuses, devices and methods of management thereof for cultivating tissue |
Also Published As
Publication number | Publication date |
---|---|
JPWO2016158337A1 (ja) | 2017-11-24 |
US20190211301A1 (en) | 2019-07-11 |
CN107406812A (zh) | 2017-11-28 |
EP3260529A1 (en) | 2017-12-27 |
US10704020B2 (en) | 2020-07-07 |
CN107406812B (zh) | 2020-02-28 |
JP6386164B2 (ja) | 2018-09-05 |
EP3260529A4 (en) | 2018-02-28 |
EP3260529B1 (en) | 2019-01-30 |
WO2016158337A1 (ja) | 2016-10-06 |
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Owner name: PANASONIC HEALTHCARE HOLDINGS CO., LTD., JAPAN Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:SEKINE, HIRONOBU;KOBAYASHI, KOICHI;REEL/FRAME:043830/0517 Effective date: 20170905 |
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Owner name: PHC HOLDINGS CORPORATION, JAPAN Free format text: CHANGE OF NAME;ASSIGNOR:PANASONIC HEALTHCARE HOLDINGS CO., LTD.;REEL/FRAME:047093/0475 Effective date: 20180402 |
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Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |