US20140031389A1 - Screening methods and pharmaceutical compositions for the treatment of inflammatory bowel diseases - Google Patents

Screening methods and pharmaceutical compositions for the treatment of inflammatory bowel diseases Download PDF

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US20140031389A1
US20140031389A1 US14/111,291 US201214111291A US2014031389A1 US 20140031389 A1 US20140031389 A1 US 20140031389A1 US 201214111291 A US201214111291 A US 201214111291A US 2014031389 A1 US2014031389 A1 US 2014031389A1
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expression
phosphorylation
inflammatory bowel
eif2a
bowel disease
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Eric Ogier-Denis
Xavier Treton
Eric Pedruzzi
Fanny Daniel
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Institut National de la Sante et de la Recherche Medicale INSERM
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A61K31/00—Medicinal preparations containing organic active ingredients
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    • A61K31/155—Amidines (), e.g. guanidine (H2N—C(=NH)—NH2), isourea (N=C(OH)—NH2), isothiourea (—N=C(SH)—NH2)
    • A—HUMAN NECESSITIES
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
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    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0306—Animal model for genetic diseases
    • A01K2267/0325—Animal model for autoimmune diseases
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8527—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic for producing animal models, e.g. for tests or diseases

Definitions

  • the present invention relates to screening methods and pharmaceutical compositions for the treatment of inflammatory bowel diseases.
  • Ulcerative colitis is a chronic intermittent and relapsing inflammatory bowel disease (IBD) of the colon characterized by superficial mucosal lesions that extend through the rectum and progress upstream.
  • IBD inflammatory bowel disease
  • the natural history of UC is characterized by the progression of colonic lesions in up to 50% of subjects. This suggests that the colonic mucosa has a “global” susceptibility to environmental factors.
  • genome-wide association studies have already identified more than 40 gene loci associated with UC, the primary defects initiating the cascade of events in colonic epithelial cells and leading to inflammation remain largely unknown.
  • Spontaneous inflammatory disease in transgenic rats expressing HLA-B27 and human beta 2m an animal model of HLA-B27-associated human disorders. Cell 63, 1099-112 (1990)). It has been suggested that the UPR might help intestinal epithelial cells cope with endoplasmic reticulum (ER) stress and thus deter pro-inflammatory pathways allowing the cell to adapt and respond to environmental changes.
  • ER endoplasmic reticulum
  • the UPR is mediated by i)—IRE1 that signals through a transcription factor X-box protein 1 (XBP-1) to activate UPR target genes, ii)—ATF6 ⁇ that induces XBP-1 mRNA and enhances the ability of the cell to cope with the load of unfolded proteins, and iii)—PERK that phosphorylates the alpha subunit of translation initiation factor 2 (eIF2 ⁇ ) which abolishes the eIF2 ⁇ -GTP-Met-tRNA i Met ternary complex formation and inhibits translation of most mRNAs.
  • This adaptation is believed to protect cells against toxic malfolded proteins that can accumulate under stress and to conserve ATP and amino acids in ER stressed cells.
  • Paradoxically, eIF2 ⁇ phosphorylation stimulates the translation of a subset of genes including ATF4, an inducer of the integrated stress response (ISR) and its downstream transcriptional target, DDIT3 (CHOP).
  • the present invention relates to screening methods and pharmaceutical compositions for the treatment of inflammatory bowel diseases.
  • the present invention relates to a method for screening a plurality of test substances useful for the prevention or treatment of an inflammatory bowel disease comprising the steps consisting of (a) testing each of the test substances for its ability to restore the integrated stress response and (b) and positively selecting the test substances capable of restoring said integrated stress response.
  • a further aspect of the invention relates to an agent capable of restoring the integrated stress response (ISR) for use in the treatment of an inflammatory bowel disease.
  • ISR integrated stress response
  • the inventors show that unaffected UC mucosa exhibit inappropriate ER stress compared to control subjects.
  • the results show unexpected impairment of the ISR pathway due to a net decrease in eIF2 ⁇ phosphorylation and thus suggest that the defect in this central stress response associated with the absence of stress granule assembly and increased expression of key components of the translational initiation machinery cause the reprogramming of protein translation in UC.
  • This is supported by a genome-wide microarray analysis of polysome-bound mRNAs isolated from the unaffected mucosa of UC subjects vs. controls.
  • These stress-modulated processes affect several key functions of the epithelial barrier which might explain the susceptibility of colonic UC mucosa to environmental stresses. Accordingly ISR represents an attractive target for new therapeutic options to maintain UC subjects in long-term remission.
  • integrated stress response As used herein the term “integrated stress response” or “ISR” has its general meaning in the art and refers to the pathway described in Schroder & Kaufman ER stress and unfolded protein response. Mut Res. 2005, 569: 29; Ron & Walter, “Signal integration in the endoplasmic reticulum unfolded protein responses”, Nat Rev Mol Cell Biol 2007, 8: 519 and in Moenner et al., “Integrated stress response in cancer”, Cancer Res. 2007, 67: 10631, The term ‘integrated stress response” is also named as the “eIF2 ⁇ pathway”.
  • the phosphorylation event of eIF2 ⁇ integrates various types of environmental and endogenous stress signals beyond ER stress, such as amino acid deprivation, exposure to double-stranded viral RNA, osmotic stress, UV light exposure, heme deficiency, hypoxia, and oxidative stress (Harding et al., (2003) “.An integrated stress response regulates amino acid metabolism and resistance to oxidative stress” Mol Cell, 11 (3), 619-33)
  • These divergent signals activate four different eIF2 ⁇ kinases including PERK (which is activated by ER stress, radiation, or hypoxia), general control nonderepressible-2 (GCN2, which is activated by uncharged tRNAs in amino acid-starved cells), heme-regulated inhibitor (HRI, which is activated by heme deficiency in erythroid precursor cells), and PKR (which is activated by double-stranded RNA and in some contexts, ER stress (Nakamura et al
  • mammalian cells activate a protective mechanism to prevent damage of vital cellular processes required for homeostasis, once the stress is relieved (Nover et al., (1989), “Cytoplasmic heat shock granules are formed from precursor particles and are associated with a specific set of mRNAs” Mol Cell Biol, 9 (3): 1298-308).
  • SGs stress granules
  • the rapid formation of stress granules (SGs) in the cytoplasm is one of the main mechanisms by which the cell inhibits translation of mRNAs encoding for “housekeeping” functions to prioritize the synthesis of chaperones and enzymes needed for the stress response (Anderson and Kedersha, (2002) “Visibly stressed: the role of eIF2, TIA-1, and stress granules in protein translation” Cell Stress Chaperones, 7 (2), 213-21).
  • the process that inhibits translation during the ER stress, and which also acts as a stimulus for SG assembly targets specifically the initiation phase of translation (Anderson and Kedersha, (2006) “RNA granules” J Cell Biol., 172 (6), 803-8).
  • SG formation by mitochondrial poisons has been documented to occur in the absence of eIF2 phosphorylation (Kedersha et al., (2002) “Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules”, Mol Biol Cell, 13 (1), 195-210). This suggests that inhibition of translation initiation by stimuli that do not induce eIF2 ⁇ phosphorylation may also be capable of inducing SG formation.
  • eIF2 ⁇ has its general meaning in the art and refers to the eukaryotic translation initiation factor 2A that is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll W L et al. (2002). “Characterization of mammalian eIF2A and identification of the yeast homolog”. J Biol Chem 277 (40): 37079-87..; Merrick W C (1992). “Mechanism and regulation of eukaryotic protein synthesis”. Microbiol Rev 56 (2): 291-315).
  • PERK has its general meaning in the art and refers to the eukaryotic translation initiation factor 2-alpha kinase 3 (Shi Y, et al. (1998) “Identification and characterization of pancreatic eukaryotic initiation factor 2 alpha-subunit kinase, PEK, involved in translational control”. Mol Cell Biol. 18(12):7499-509).
  • GCN2 has its general meaning in the art and refers to the eukaryotic translation initiation factor 2 alpha kinase 4 (Berlanga J J et al. (1999) “Characterization of a mammalian homolog of the GCN2 eukaryotic initiation factor 2alpha kinase”. Eur J Biochem.;265(2):754-62).
  • ATF4 has its general meaning in the art and refers to the Activating transcription factor 4 (tax-responsive enhancer element B67) (Tsujimoto Aet al. (1991). “Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I”. Journal of Virology 65 (3): 1420-6.. Karpinski B Aet al. (1992). “Molecular cloning of human CREB-2: an ATF/CREB transcription factor that can negatively regulate transcription from the cAMP response element”. Proceedings of the National Academy of Sciences of the United States of America 89 (11): 4820-4.)
  • GADD34 for “DNA damage-inducible protein 34” or MyD116 or PPP1R15A has its general meaning in the art and refers to a protein inhibitor 1 (I-1) interacting protein that associates with the C terminus of human I-1.
  • I-1 protein inhibitor 1
  • GADD34 whose expression in mammalian cells is elevated by growth arrest, DNA damage, and other forms of cell stress has structural homology to a region of the herpes simplex virus (HSV-1) neurovirulence factor ICP-345, previously shown to bind PP1.
  • HSV-1 herpes simplex virus
  • ICP-345 neurovirulence factor
  • GADD34 is an effector of a negative feedback loop that terminates UPR signaling and recruits a catalytic subunit of protein phosphatase 1 (PP1c) to dephosphorylate eIF2 ⁇ .
  • protein phosphatase 1 denotes a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits.
  • an inhibitor of the formation of the PP1/GADD34 complex denotes an inhibitor able to compete in the ⁇ M range with GADD34 to form a complex with PP1 and thereby render said complex non functional, or to block GADD34 expression or to render GADD34 structurally inactive.
  • an inhibitor of the formation of the PP1/GADD34 complex will have an EC50 not greater than 50 ⁇ M and preferably not greater than 25 ⁇ M.
  • inflammatory bowel disease has its general meaning in the art and refers to any inflammatory disease that affects the bowel.
  • the term includes but is not limited to ulcerative colitis, Crohn's disease in a state that affect specifically the colon with or without ileitis, microscopic colitis (lymphocytic colitis and collagenous colitis), infectious colitis caused by bacteria or by virus, radiation colitis, ischemic colitis, pediatric colitis, undetermined colitis, and functional bowel disorders (described symptoms without evident anatomical abnormalities).
  • a subject denotes a mammal, such as a rodent, a feline, a canine, and a primate.
  • a subject according to the invention is a human.
  • An aspect of the invention relates to a method for screening a plurality of test substances useful for the prevention or treatment of an inflammatory bowel disease comprising the steps consisting of (a) testing each of the test substances for its ability to restore the integrated stress response and (b) and positively selecting the test substances capable of restoring said integrated stress response.
  • step (a) of the screening method may consist in determining whether the test substances i) increase the phosphorylation of eIF2a ⁇ ii) activate the expression of ATF4 gene, ii) activate the expression of some genes targeted by ATF4 as depicted in Table 1, iii) activate the kinases that promote eIF2 ⁇ phosphorylation, iv) inhibit the dephosphorylation of phosphorylated eIF2 ⁇ .
  • RANKL receptor activator Yang & Karsenty, of NF-kB 2004 E selectin Liang & Hai, 1997; VEGF Roybal et al. 2005 Gadd153 Fawcett et al 1999 Asparagine synthase Chen, & Kilberg, 2004 TRB3 Ohoka et al 2005 Nrf2 and HO1 Cullinan and Diehl 2006.
  • the present invention is directed to a method for screening a plurality of test substances useful for the prevention or treatment of an inflammatory bowel disease, which comprises the steps of i) testing each of the test substances for its ability to activate the expression of ATF4 gene, and ii) identifying the test substance which activates the expression of ATF4, thereby to identify a test substance useful as a preventive or therapeutic agent for an inflammatory bowel disease.
  • the invention is directed to a method, which comprises the steps of i) contacting the test substance or each of the test substances with a cell transfected with a reporter gene operatively linked to all or part of the promoter of the ATF4 gene, ii) assessing the level of expression of said reporter gene, and iii) identifying the test substance which activates the expression of said reporter gene, thereby to identify a test substance useful as a preventive or therapeutic agent for an inflammatory bowel disease.
  • the reporter gene encodes one of the groups consisting of GFP, CAT, GAL, LUC, and GUS.
  • the cell is one of the groups consisting of a CHO, BHK, 3T3, and HEK293 cell line.
  • the invention is directed to a method, which comprises the steps of i) contacting the test substance or each of the test substances with a cell capable of expressing the ATF4 gene, ii) assessing the level of expression of said gene, and iii) identifying the test substance which activates the expression of said gene, thereby to identify a test substance useful as a preventive or therapeutic agent for an inflammatory bowel disease.
  • the level of expression is assessed by determining the level of transcription of said gene.
  • the determination of the level of translation of said gene is effected by means of an immunoassay.
  • the invention is also directed to a method for screening a plurality of test substances useful for the prevention or treatment of an inflammatory bowel disease, which comprises the steps of i) testing each of the test substances for its ability to activate the expression of a gene depicted in Table 1, and ii) identifying the test substance which activates the expression of said gene, thereby to identify a test substance useful as a preventive or therapeutic agent for an inflammatory bowel disease.
  • the method comprises the steps of i) contacting the test substance or each of the test substances with a cell transfected with a reporter gene operatively linked to all or part of the promoter of said gene depicted in Table 1, ii) assessing the level of expression of said reporter gene, and iii) identifying the test substance which activates the expression of said reporter gene, thereby to identify a test substance useful as a preventive or therapeutic agent for an inflammatory bowel disease.
  • the reporter gene encodes one of the groups consisting of GFP, CAT, GAL, LUC, and GUS.
  • the cell is one of the groups consisting of a CHO, BHK, 3T3, and HEK293 cell line.
  • Activation of expression of any gene can be assessed by determining either the level of transcription or the level of translation in the presence of the test substance in comparison with control assays performed in the absence of the test substance.
  • Such assays are well known in the art and are depicted herein after. For example such assays may be performed on cells capable of expressing the gene (host cells).
  • the invention is also directed to a method for screening a plurality of test substances useful for the prevention or treatment of an inflammatory bowel disease, which comprises the steps of i) testing each of the test substances for its ability to increase phosphorylation of eIF2 ⁇ , and ii) identifying the test substance which increases phosphorylation of eIF2 ⁇ , thereby to identify a test substance useful as a preventive or therapeutic agent for an inflammatory bowel disease.
  • the invention is also directed to the method, which comprises the steps of i) contacting the test substance or each of the test substances with a cell capable of expressing eIF2 ⁇ , ii) assessing the level of phosphorylation of eIF2 ⁇ , and iii) identifying the test substance which increases the phosphorylation of eIF2 ⁇ , thereby to identify a test substance useful as a preventive or therapeutic agent for an inflammatory bowel disease.
  • the assessment of the level of phosphorylation of eIF2 ⁇ is effected by an immunoassay using an antibody that specifically recognizes the phosphorylated form of eIF2 ⁇ .
  • the assessment of the level of phosphorylation of eIF2 ⁇ is effected by tracking the covalent binding of a radiolabeled phosphate group to eIF2 ⁇ ,
  • the invention is also directed to a method for screening a plurality of test substances useful for the prevention or treatment of an inflammatory bowel disease, which comprises the steps of i) testing each of the test substances for its ability to inhibit the dephosphorylation of eIF2 ⁇ , and ii) identifying the test substance which inhibits the dephosphorylation of eIF2 ⁇ , thereby to identify a test substance useful as a preventive or therapeutic agent for an inflammatory bowel disease.
  • the invention is directed to a method, which comprises the steps of i) contacting the test substance or each of the test substances with a cell-free composition containing GADD34 and PP1c proteins in the form of a purified complex and eIF2 ⁇ , in a phosphorylated form, ii) assessing the level of phosphorylation of eIF2 ⁇ , in comparison with the level of phosphorylation determined in the absence of test substances, in a cell-free composition containing GADD34 and PP1c proteins in the form of a purified complex and eIF2 ⁇ , in a phosphorylated form, and iii) identifying the test substance which provides a higher level of phosphorylation of eIF2 ⁇ , in comparison with the level of phosphorylation determined in the absence of test substance, thereby to identify a test substance useful as a preventive or therapeutic agent for an inflammatory bowel disease.
  • the assessment of the level of phosphorylation of eIF2 ⁇ is effected by an immunoassay using an antibody that specifically recognizes the phosphorylated form of eIF2 ⁇ .
  • the assessment of the level of phosphorylation of eIF2 ⁇ is effected by tracking the covalent binding of a radiolabeled phosphate group to eIF2 ⁇ ,
  • the invention is also directed to a method for screening a plurality of test substances useful for the prevention or treatment of an inflammatory bowel disease, which comprises the steps of i) testing each of the test substances for its ability to activate an eIF2 ⁇ ,a kinase and ii) identifying the test substance which activates an eIF2 ⁇ , kinase, thereby to identify a test substance useful as a preventive or therapeutic agent for an inflammatory bowel disease.
  • the kinase is PERK.
  • the kinase is GCN2.
  • the kinase is HRI.
  • the kinase is PKR.
  • host-expression vector systems may be utilized to express the genes used in the assays of this invention. These include, but are not limited to, mammalian cell systems such as human cell lines derived from colon adenocarcinoma including HT-29, Caco-2, SW480, HTC116 cell lines.
  • the mammalian cell systems may harbour recombinant expression constructs containing promoters derived from the genome of mammalian cells or from mammalian viruses (e.g., the adenovirus late promoter or the vaccine virus 7.5K promoter).
  • Additional host-expression vector systems include, but are not limited to, microorganisms such as bacteria (e.g., E. 5 coli or B. subtilis ) transformed with recombinant bacteriophage DNA, plasmid DNA, or cosmid DNA expression vectors containing PTK or adaptor protein coding sequences; yeast (e.g., Saccharomyces, Pichia ) transformed with recombinant yeast expression vectors containing the protein or peptide oding sequences; insect cell systems, such as Sf9 or Sf21 infected with recombinant virus expression vectors (e.g., baculovirus) containing the protein or peptide coding sequences; amphibian cells, such as Xenopus oocytes; or plant cell systems infected with recombinant virus express—15 sion vectors (e.g., cauliflower mosaic virus, CMV; tobacco mosaic virus, TMV) or transformed with recombinant plamid expression vectors (e.g., Ti
  • DNA encoding proteins to be assayed can be transiently or stably expressed in the cell lines by several methods known in the art, such as, calcium phosphate-mediated, DEAE-dextran mediated, liposomal-mediated, viral-mediated, electroporation-mediated and microinjection delivery. Each of these methods may require optimization of assorted experimental parameters depending on the DNA, cell line, and the type of assay to be subsequently employed.
  • Determination of the expression level of a gene can be performed by a variety of techniques. Generally, the expression level as determined is a relative expression level.
  • the determination comprises contacting the sample with selective reagents such as probes, primers or ligands, and thereby detecting the presence, or measuring the amount, of polypeptide or nucleic acids of interest originally in the sample.
  • Contacting may be performed in any suitable device, such as a plate, microtiter dish, test tube, well, glass, column, and so forth
  • the contacting is performed on a substrate coated with the reagent, such as a nucleic acid array or a specific ligand array.
  • the substrate may be a solid or semi-solid substrate such as any suitable support comprising glass, plastic, nylon, paper, metal, polymers and the like.
  • the substrate may be of various forms and sizes, such as a slide, a membrane, a bead, a column, a gel, etc.
  • the contacting may be made under any condition suitable for a detectable complex, such as a nucleic acid hybrid or an antibody-antigen complex, to be formed between the reagent and the nucleic acids or polypeptides of the sample.
  • the expression level may be determined by determining the quantity of mRNA.
  • the nucleic acid contained in the samples e.g., cell or tissue prepared from the subject
  • the samples e.g., cell or tissue prepared from the subject
  • the extracted mRNA is then detected by hybridization (e. g., Northern blot analysis) and/or amplification (e.g., RT-PCR).
  • hybridization e. g., Northern blot analysis
  • amplification e.g., RT-PCR
  • RT-PCR e.g., RT-PCR
  • quantitative or semi-quantitative RT-PCR is preferred. Real-time quantitative or semi-quantitative RT-PCR is particularly advantageous.
  • LCR ligase chain reaction
  • TMA transcription-mediated amplification
  • SDA strand displacement amplification
  • NASBA nucleic acid sequence based amplification
  • Nucleic acids having at least 10 nucleotides and exhibiting sequence complementarity or homology to the mRNA of interest herein find utility as hybridization probes or amplification primers. It is understood that such nucleic acids need not be identical, but are typically at least about 80% identical to the homologous region of comparable size, more preferably 85% identical and even more preferably 90-95% identical. In certain embodiments, it will be advantageous to use nucleic acids in combination with appropriate means, such as a detectable label, for detecting hybridization. A wide variety of appropriate indicators are known in the art including, fluorescent, radioactive, enzymatic or other ligands (e. g. avidin/biotin).
  • Probes typically comprise single-stranded nucleic acids of between 10 to 1000 nucleotides in length, for instance of between 10 and 800, more preferably of between 15 and 700, typically of between 20 and 500.
  • Primers typically are shorter single-stranded nucleic acids, of between 10 to 25 nucleotides in length, designed to perfectly or almost perfectly match a nucleic acid of interest, to be amplified.
  • the probes and primers are “specific” to the nucleic acids they hybridize to, i.e. they preferably hybridize under high stringency hybridization conditions (corresponding to the highest melting temperature Tm, e.g., 50% formamide, 5x or 6x SCC. SCC is a 0.15 M NaCl, 0.015 M Na-citrate).
  • the nucleic acid primers or probes used in the above amplification and detection method may be assembled as a kit.
  • a kit includes consensus primers and molecular probes.
  • a preferred kit also includes the components necessary to determine if amplification has occurred.
  • the kit may also include, for example, PCR buffers and enzymes; positive control sequences, reaction control primers; and instructions for amplifying and detecting the specific sequences.
  • the expression level is determined by DNA chip analysis.
  • DNA chip or nucleic acid microarray consists of different nucleic acid probes that are chemically attached to a substrate, which can be a microchip, a glass slide or a microsphere-sized bead.
  • a microchip may be constituted of polymers, plastics, resins, polysaccharides, silica or silica-based materials, carbon, metals, inorganic glasses, or nitrocellulose.
  • Probes comprise nucleic acids such as cDNAs or oligonucleotides that may be about 10 to about 60 base pairs.
  • a sample from a test subject optionally first subjected to a reverse transcription, is labelled and contacted with the microarray in hybridization conditions, leading to the formation of complexes between target nucleic acids that are complementary to probe sequences attached to the microarray surface.
  • the labelled hybridized complexes are then detected and can be quantified or semi-quantified. Labelling may be achieved by various methods, e.g. by using radioactive or fluorescent labelling. Many variants of the microarray hybridization technology are available to the man skilled in the art (see e.g. the review by Hoheisel, Nature Reviews, Genetics, 2006, 7:200-210)
  • Other methods for determining the expression level of said genes include the determination of the quantity of proteins encoded by said genes.
  • Such methods comprise contacting a biological sample with a binding partner capable of selectively interacting with a marker protein present in the sample.
  • the binding partner is generally an antibody that may be polyclonal or monoclonal, preferably monoclonal.
  • the presence of the protein can be detected using standard electrophoretic and immunodiagnostic techniques, including immunoassays such as competition, direct reaction, or sandwich type assays.
  • immunoassays include, but are not limited to, Western blots; agglutination tests; enzyme-labeled and mediated immunoassays, such as ELISAs; biotin/avidin type assays; radioimmunoassays; immunoelectrophoresis; immunoprecipitation, etc.
  • the reactions generally include revealing labels such as fluorescent, chemiluminescent, radioactive, enzymatic labels or dye molecules, or other methods for detecting the formation of a complex between the antigen and the antibody or antibodies reacted therewith.
  • the aforementioned assays generally involve separation of unbound protein in a liquid phase from a solid phase support to which antigen-antibody complexes are bound.
  • Solid supports which can be used in the practice of the invention include substrates such as nitrocellulose (e. g., in membrane or microtiter well form); polyvinylchloride (e. g., sheets or microtiter wells); polystyrene latex (e.g., beads or microtiter plates); polyvinylidine fluoride; diazotized paper; nylon membranes; activated beads, magnetically responsive beads, and the like.
  • an ELISA method can be used, wherein the wells of a microtiter plate are coated with an antibody against the protein to be tested. A biological sample containing or suspected of containing the marker protein is then added to the coated wells. After a period of incubation sufficient to allow the formation of antibody-antigen complexes, the plate (s) can be washed to remove unbound moieties and a detectably labeled secondary binding molecule added. The secondary binding molecule is allowed to react with any captured sample marker protein, the plate washed and the presence of the secondary binding molecule detected using methods well known in the art.
  • the levels of phosphorylation of proteins can be assessed by various methods, including immunoassays or radiolabeling.
  • the levels of phosphorylation of target proteins can be assessed by various methods, including immunoassays or radiolabelling. Specifically, the increase of phosphorylation of eIF2 ⁇ may be measured, activation of the kinases that promote eIF2 ⁇ phosphorylation may be assayed, and inhibition of dephosphorylation of phosphorylated eIF2 ⁇ may also be determined by these techniques.
  • the level of phosphorylation of a protein is assessed by utilizing a binding partner, which should be highly specific for the target protein. It is preferred that the binding partner be an antibody. It is preferably generated against a unique epitope of the substrate. In an alternative, the binding partner should be specific for the phosphorylated form of the target protein.
  • the detection procedure used to assess the phosphorylation state of eIF2 ⁇ may for instance employ an anti-phosphoserine antibody or a peptide that recognizes and binds to phosphorylated serines.
  • the detection antibody is preferably a polyclonal antibody to maximize the signal, but may also be specific monoclonal antibodies which have been optimized for signal generation.
  • levels of eIF2 ⁇ phosphorylated on serine 51 can be measured by immunoblot or immunocytochemistry utilizing a commercially available antibodies, for example, product #9721 from Cell Signalling Technology.
  • the commercially available antisera to phosphorylated eIF2 ⁇ may be used to develop high throughput screening assays for test substances that promote the accumulation of phosphorylated eIF2 ⁇ .
  • inhibition of dephosphorylation of eIF2a on serine 51 may be assayed by screening a test substance's ability to inhibit the activity of the PP1c and GADD34 complex (Novoa, I., et al. (2001). “Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2 ⁇ ”. J. Cell. Biol. 28;153(5):1011-22).
  • the PP1c and GADD34 complex is active in vitro, and its activity may be reconstituted using recombinant proteins.
  • a cell-free assay may be used with the PP1c/GADD34 complex in combination with phosphorylated eIF2 ⁇ and test substances.
  • dephosphorylation of eIF2 ⁇ by the PP1c/GADD34 complex and inhibition of this dephosphorylation by a test substance may be monitored by measuring the decrease in phosphorylated eIF2 ⁇ signal.
  • activation of the eIF2 ⁇ kinases may be measured.
  • Activation of the kinases is associated with an autophosphorylation event on known residues in the kinase (e.g., threonine 898 of mouse GCN2 and threonine 980 of mouse PERK).
  • antisera which recognize the phosphorylated and activated forms of the kinases
  • activation of the kinases may be detected using immunoblot or immunochemistry, such as with an ELISA.
  • Antisera for the phosphorylated forms of the kinases PERK and GCN2 have been developed. (Harding, H., et al. (2000). “Regulated translation initiation controls stress induced gene expression in mammalian cells”. Mol. Cell 6, 1099-1108).
  • immunoassays may be replaced by the detection of radiolabeled phosphate according to a standard technique. This involves incubating cells with the test substances and radiolabeled phosphate, lysing the cells, separating cellular protein components of the lysate using as SDS-polyacrylamide gel (SDS-PAGE) technique, in either one or two dimensions, and detecting the presence of phosphorylated proteins by exposing X-ray film.
  • SDS-PAGE SDS-polyacrylamide gel
  • the phosphorylation of a protein may also be conveniently detected by migration on an electrophoresis gel and Western blot, to thereby observe whether a shift of the molecular weight of the protein occurs, a phosphorylated protein being heavier than the corresponding non-phosphorylated form.
  • the test substance of may be selected from the group consisting of peptides, peptidomimetics, small organic molecules, antibodies, aptamers or nucleic acids.
  • the test substance according to the invention may be selected from a library of compounds previously synthesized, or a library of compounds for which the structure is determined in a database, or from a library of compounds that have been synthesized de novo.
  • test substance may be selected form small organic molecules.
  • small organic molecule refers to a molecule of size comparable to those organic molecules generally sued in pharmaceuticals.
  • the above assays may be performed using high throughput screening techniques for identifying test substances for developing drugs that may be useful to the treatment or prevention of an inflammatory bowel disease.
  • High throughput screening techniques may be carried out using multi-well plates (e.g., 96-, 389-, or 1536-well plates), in order to carry out multiple assays using an automated robotic system.
  • multi-well plates e.g., 96-, 389-, or 1536-well plates
  • large libraries of test substances may be assayed in a highly efficient manner.
  • a preferred strategy for identifying test substances starts with cultured cells transfected with a reporter gene fused to the promoter of any gene that is activated by the stress response pathway.
  • stably-transfected HT-29 cells growing in wells of micro-titer plates can be adapted to high through-put screening of libraries of compounds.
  • Compounds in the library will be applied one at a time in an automated fashion to the wells of the microtitre dishes containing the transgenic cells described above.
  • test substances which activate one of the target genes are identified, it is preferable to then determine their site of action in the Integrated Stress Response pathway. It is particularly useful to define the site of action for the development of more refined assays for in order to optimize the target substance. Assays to determine the site of action of the target substance in the ISR may be carried out using high throughput techniques.
  • the ELISA-based assay for measuring ATF4 translation is particularly adapted to rapid high throughput screening.
  • an ELISA assay for measuring phosphorylated eIF2 ⁇ by means of commercially available antiserum, may be developed for high throughput screening.
  • antisera to phorphorylated eIF2 ⁇ kinases may be advantageously used in ELISA-based high throughput screens to focus on upstream components of the pathway.
  • ELISA-type assays may be performed in microtitre plates. See, for example, Peraldi et al., (1992), J. Biochem. 285: 71-78; Schraag, et al., (1993), Analytical Biochemistry 211: 233-239; Cleavland, (1990), Analytical Biochemistry 190: 249-253; Farley, (1992), Analytical Biochemistry 203: 151-157; and Lczaro, (1991), Analytical Biochemistry 192: 257-261.
  • the rapid and quantitative assays systems described in U.S. Pat. No. 5,763,198.
  • two embodiments may be contemplated as follows.
  • the extent of phosphorylation of a target protein may be measured by exposing cells that express the target protein to a test substance and, thereafter, lysing the cell to release the cellular contents.
  • the target protein is isolated by incubating the cell lysate with a binding partner to a solid support and thereafter washing away non-bound cellular components.
  • a detection procedure is performed to assess the presence or absence of phosphorylated residues on the protein as compared to lysates of control cells, which were not exposed to the test substance.
  • the binding partner may be directed against the phosphorylated forms of the target protein, so that the steps of isolation and of detection of phosphorylation are performed simultaneously.
  • test substance to a whole cell allows for the evaluation of its activity in the natural context in which the test substance may act.
  • radioactive labeling of the target cell proteins is not required in the assay. Because this assay can readily be performed in a microtitre plate format, the assays described can be performed by an automated robotic system, allowing for testing of large numbers of test samples within a reasonably short time frame.
  • An alternative embodiment of the invention relates to methods for determining the effect of a test substance on the ability of kinases to phosphorylate eIF2 ⁇ in a cell-free system.
  • the test substance is added to a reaction mixture containing the kinase and eIF2 ⁇ bound to a solid support by an antibody.
  • the kinase reaction may be initiated by the addition of ATP.
  • a detection procedure as described herein is performed on the substance to assess the presence or absence of the phosphorylated residues, and results are compared to those obtained for controls, i.e., reaction mixtures to which the test substance was not added.
  • the assays of the invention can be used as a screen to assess the activity of a previously untested compound or extract, in which case a single concentration is tested and compared to controls. These assays can also be used to assess the relative potency of a compound by testing a range of concentrations, in a range of 100 ⁇ M to 1 ⁇ M, for example, and computing the concentration at which the amount of phosphorylation is increased by one-half (IC50) compared to controls.
  • the whole cell assay of the invention described herein can be performed, for example, by utilizing pre-packaged kits comprising any or all of the reagents of the assay, such as a solid phase coated with a binding partner to a protein of interest, or a detection molecule.
  • the cell-free assays of the invention may be performed, for example, by utilizing pre-packaged kits comprising any or all of the reagents of the assay.
  • a further aspect of the invention relates to an agent capable of restoring the integrated stress response (ISR) for use in the treatment of an inflammatory bowel disease.
  • ISR integrated stress response
  • said agent is able to i) increase the phosphorylation of eIF2 ⁇ , ii) activate the expression of ATF4 gene, ii) activate the expression of some genes targeted by ATF4 as depicted in Table 1, iii) activate the kinases that promote eIF2 ⁇ phosphorylation, iv) inhibit the dephosphorylation of phosphorylated eIF2 ⁇ and v) promote stress granule formation.
  • a further aspect of the invention relates to an agent capable of restoring the integrated stress response (ISR) for use in maintaining subjects affected by an inflammatory bowel disease in a long term remission after standard treatment or surgery.
  • ISR integrated stress response
  • standard treatment of the inflammatory bowel diseases, especially ulcerative colitis include administration of corticosteroids, immunosuppressive drugs, aminosalicylates sulfasalazine, such as Mesalazine (also known as 5-aminosalicylic acid, mesalamine, or 5-ASA.
  • Brand name formulations include Apriso, Asacol, Pentasa, Mezavant, Lialda, Fivasa, Rovasa and Salofalk.), Sulfasalazine (also known as Azulfidine), Balsalazide (also known as Colazal or Colazide (UK)), Olsalazine (also known as Dipentum), immunosuppressors (azathioprine, 6-mercaptopurine, methotrexate, rapamycine, cyclosporine and tacrolimus) or biological treatments such as Infliximab, Visilizumab, Adalimumab, or Vedolizumab.
  • the agent that inhibits the dephosphorylation of phosphorylated eIF2 ⁇ is salubrinal (3-phenyl-N-[2,2,2-trichloro-1-[[(8-quinolinylamino)thioxomethyl]amino]ethyl]-2-propenamide) (Boyce et al (2005) “A selective inhibitor of elF2alpha dephosphorylation protects cells from ER stress”. Science 307 935. Long et al (2005) “Structure-activity relationship studies of salubrinal lead to its active biotinylated derivative”. Bioorg.Med.Chem.Lett. 15 3849).
  • Salubrinal is a cell-permeable, selective inhibitor of cellular phosphatase complexes that dephosphorylate eIF2 ⁇ . Salubrinal is available from Alexis Biochemicals or Tocris Bioscience (Cat No. 2347), or other source as known to one of skill in the art.
  • the agent that inhibits the dephosphorylation of phosphorylated eIF2 ⁇ is guanabenz (2-(2,6-dichlorobenzylidene) hydrazinecarboximidamide).
  • Guanabenz is a small-molecule which bound to a regulatory subunit of protein phosphatase 1, PPP1R15A/GADD34, selectively disrupting the stressinducedstress induced dephosphorylation of the a subunit of translation initiation factor 2 (eIF2 ⁇ ) (Tsaytler P, Harding HP, Ron D, Bertolotti “A.Selective inhibition of a regulatory subunit of protein phosphatase 1 restores proteostasis.”. Science. 2011 Apr 1;332(6025):91-4)
  • the agent that inhibits the dephosphorylation of phosphorylated eIF2 is an inhibitor of GADD34 or PP1 gene expression.
  • Inhibitors of expression for use in the present invention may be based on anti-sense oligonucleotide constructs.
  • Anti-sense oligonucleotides including anti-sense RNA molecules and anti-sense DNA molecules, would act to directly block the translation of GADD34 or PP1 mRNA by binding thereto and thus preventing protein translation or increasing mRNA degradation, thus decreasing the level of GADD34 or PP1, and thus activity, in a cell.
  • antisense oligonucleotides of at least about 15 bases and complementary to unique regions of the mRNA transcript sequence encoding GADD34 or PP1 can be synthesized, e.g., by conventional phosphodiester techniques and administered by e.g., intravenous injection or infusion.
  • Methods for using antisense techniques for specifically inhibiting gene expression of genes whose sequence is known are well known in the art (e.g. see U.S. Pat. Nos. 6,566,135; 6,566,131; 6,365,354; 6,410,323; 6,107,091; 6,046,321; and 5,981,732).
  • Small inhibitory RNAs siRNAs
  • siRNAs can also function as inhibitors of expression for use in the present invention.
  • GADD34 or PP1 gene expression can be reduced by contacting a subject or cell with a small double stranded RNA (dsRNA), or a vector or construct causing the production of a small double stranded RNA, such that GADD34 or PP1 gene expression is specifically inhibited (i.e. RNA interference or RNAi).
  • dsRNA small double stranded RNA
  • RNAi RNA interference or RNAi.
  • siRNA sequences advantageously comprise at least twelve contiguous dinucleotides or their derivatives.
  • RNA derivatives with respect to the present nucleic acid sequences refers to a nucleic acid having a percentage of identity of at least 90% with erythropoietin or fragment thereof, preferably of at least 95%, as an example of at least 98%, and more preferably of at least 98%.
  • percentage of identity between two nucleic acid sequences, means the percentage of identical nucleic acid, between the two sequences to be compared, obtained with the best alignment of said sequences, this percentage being purely statistical and the differences between these two sequences being randomly spread over the nucleic acid acids sequences.
  • “best alignment” or “optimal alignment”, means the alignment for which the determined percentage of identity (see below) is the highest. Sequences comparison between two nucleic acids sequences are usually realized by comparing these sequences that have been previously align according to the best alignment; this comparison is realized on segments of comparison in order to identify and compared the local regions of similarity. The best sequences alignment to perform comparison can be realized, beside by a manual way, by using the global homology algorithm developed by Smith and Waterman (Ad. App. Math., vol.2, p:482, 1981), by using the local homology algorithm developped by Neddleman and Wunsch (J. Mol. Biol., vol.48, p:443, 1970), by using the method of similarities developed by Pearson and Lipman (Proc. Natl.
  • the identity percentage between two sequences of nucleic acids is determined by comparing these two sequences optimally aligned, the nucleic acids sequences being able to comprise additions or deletions in respect to the reference sequence in order to get the optimal alignment between these two sequences.
  • the percentage of identity is calculated by determining the number of identical position between these two sequences, and dividing this number by the total number of compared positions, and by multiplying the result obtained by 100 to get the percentage of identity between these two sequences.
  • shRNAs short hairpin RNAs
  • shRNAs can also function as inhibitors of expression for use in the present invention.
  • Ribozymes can also function as inhibitors of expression for use in the present invention.
  • Ribozymes are enzymatic RNA molecules capable of catalyzing the specific cleavage of RNA.
  • the mechanism of ribozyme action involves sequence specific hybridization of the ribozyme molecule to complementary target RNA, followed by endonucleolytic cleavage.
  • Engineered hairpin or hammerhead motif ribozyme molecules that specifically and efficiently catalyze endonucleolytic cleavage of GADD34 or PP1 mRNA sequences are thereby useful within the scope of the present invention.
  • ribozyme cleavage sites within any potential RNA target are initially identified by scanning the target molecule for ribozyme cleavage sites, which typically include the following sequences, GUA, GUU, and GUC. Once identified, short RNA sequences of between about 15 and 20 ribonucleotides corresponding to the region of the target gene containing the cleavage site can be evaluated for predicted structural features, such as secondary structure, that can render the oligonucleotide sequence unsuitable.
  • antisense oligonucleotides and ribozymes useful as inhibitors of expression can be prepared by known methods. These include techniques for chemical synthesis such as, e.g., by solid phase phosphoramadite chemical synthesis. Alternatively, anti-sense RNA molecules can be generated by in vitro or in vivo transcription of DNA sequences encoding the RNA molecule. Such DNA sequences can be incorporated into a wide variety of vectors that incorporate suitable RNA polymerase promoters such as the T7 or SP6 polymerase promoters. Various modifications to the oligonucleotides of the invention can be introduced as a means of increasing intracellular stability and half-life.
  • Possible modifications include but are not limited to the addition of flanking sequences of ribonucleotides or deoxyribonucleotides to the 5′ and/or 3′ ends of the molecule, or the use of phosphorothioate or 2′-O-methyl rather than phosphodiesterase linkages within the oligonucleotide backbone.
  • Antisense oligonucleotides, siRNAs, shRNAs and ribozymes of the invention may be delivered in vivo alone or in association with a vector.
  • a “vector” is any vehicle capable of facilitating the transfer of the antisense oligonucleotide, siRNA, shRNA or ribozyme nucleic acid to the cells and preferably cells expressing GADD34 or PP1.
  • the vector transports the nucleic acid to cells with reduced degradation relative to the extent of degradation that would result in the absence of the vector.
  • the vectors useful in the invention include, but are not limited to, plasmids, phagemids, viruses, other vehicles derived from viral or bacterial sources that have been manipulated by the insertion or incorporation of the antisense oligonucleotide, siRNA, shRNA or ribozyme nucleic acid sequences.
  • Viral vectors are a preferred type of vector and include, but are not limited to nucleic acid sequences from the following viruses: retrovirus, such as moloney murine leukemia virus, harvey murine sarcoma virus, murine mammary tumor virus, and rous sarcoma virus; adenovirus, adeno-associated virus; SV40-type viruses; polyoma viruses; Epstein-Barr viruses; papilloma viruses; herpes virus; vaccinia virus; polio virus; and RNA virus such as a retrovirus.
  • retrovirus such as moloney murine leukemia virus, harvey murine sarcoma virus, murine mammary tumor virus, and rous sarcoma virus
  • adenovirus adeno-associated virus
  • SV40-type viruses polyoma viruses
  • Epstein-Barr viruses Epstein-Barr viruses
  • papilloma viruses herpes virus
  • vaccinia virus
  • Non-cytopathic viruses include retroviruses (e.g., lentivirus), the life cycle of which involves reverse transcription of genomic viral RNA into DNA with subsequent proviral integration into host cellular DNA. Retroviruses have been approved for human gene therapy trials. Most useful are those retroviruses that are replication-deficient (i.e., capable of directing synthesis of the desired proteins, but incapable of manufacturing an infectious particle). Such genetically altered retroviral expression vectors have general utility for the high-efficiency transduction of genes in vivo.
  • viruses for certain applications are the adenoviruses and adeno-associated (AAV) viruses, which are double-stranded DNA viruses that have already been approved for human use in gene therapy.
  • AAV adeno-associated virus
  • 12 different AAV serotypes AAV1 to 12
  • Recombinant AAV are derived from the dependent parvovirus AAV2 (Choi, V W J Virol 2005; 79:6801-07).
  • the adeno-associated virus type 1 to 12 can be engineered to be replication deficient and is capable of infecting a wide range of cell types and species (Wu, Z Mol Ther 2006; 14:316-27).
  • the adeno-associated virus can integrate into human cellular DNA in a site-specific manner, thereby minimizing the possibility of insertional mutagenesis and variability of inserted gene expression characteristic of retroviral infection.
  • wild-type adeno-associated virus infections have been followed in tissue culture for greater than 100 passages in the absence of selective pressure, implying that the adeno-associated virus genomic integration is a relatively stable event.
  • the adeno-associated virus can also function in an extrachromosomal fashion.
  • Plasmid vectors have been extensively described in the art and are well known to those of skill in the art. See e.g. Sambrook et al., 1989. In the last few years, plasmid vectors have been used as DNA vaccines for delivering antigen-encoding genes to cells in vivo. They are particularly advantageous for this because they do not have the same safety concerns as with many of the viral vectors. These plasmids, however, having a promoter compatible with the host cell, can express a peptide from a gene operatively encoded within the plasmid.
  • Plasmids may be delivered by a variety of parenteral, mucosal and topical routes.
  • the DNA plasmid can be injected by intramuscular, intradermal, subcutaneous, or other routes. It may also be administered by intranasal sprays or drops, rectal suppository and orally.
  • the plasmids may be given in an aqueous solution, dried onto gold particles or in association with another DNA delivery system including but not limited to liposomes, dendrimers, cochleate and microencap sulation.
  • the antisense oligonucleotide, siRNA, shRNA or ribozyme nucleic acid sequence is under the control of a heterologous regulatory region, e.g., a heterologous promoter.
  • a heterologous promoter e.g., a heterologous promoter.
  • the promoter can also be, e.g., a viral promoter, such as CMV promoter or any synthetic promoters.
  • the agent that promotes stress granule formation is pateamine A.
  • Pateamine was first isolated from the marine sponge Mycale found off the shores of New Zealand. Northcote, P. T. et al, Tetrahedron Lett., 32:6411-6414 (1991). The natural form bears a thiazole and an E,Z-dienoate within a 19-membered macrocycle and a trienylamine side chain. Two additional pateamines, pateamines B and C, were also isolated. Their structures differ from pateamine A only in the nature of the terminal group of the trienylamine side chain. The structure for all three isolated natural forms is shown below:
  • the agent that promotes stress granule formation is a derivative of pateamine A which as the general formula:
  • A-B is ethane, (E) and (Z)-ethene, (E) and (Z)-substituted ethene, ethyne,
  • K is hydrogen or C1-C3 alkyl
  • Q is NH or O
  • X is hydrogen, hydroxy, alkoxy, alkyl, aminocarbonyl, amino, alkylamino, dialkylamino, alkoxycarbonylamino,
  • Y is S, NH, or O
  • Z is hydrogen, hydroxy, aminocarbonyl, alkylamino, dialkylamino, alkoxycarbonylamino, but not t-butoxycarbonylamino when R4 is dimethylamino,
  • R1 is hydrogen or C1-C3 alkyl
  • R is selected from the following:
  • R2 is optionally substituted with one or more substituents selected from alkyl, alkylhydroxy, alkylalkoxy, alkylamino, alkylaminoalkyl, or alkylaminodialkyl;
  • R3 is optionally substituted with one or more substituents selected from hydrogen, alkyl, alkyenyl, ankynyl, hydroxy, alkoxy, amino, alkylamino, dialkylamino, trifluromethane, or fluoro; and
  • R4 is optionally substituted with one or more substituents selected from hydrogen, alkyl, alkyenyl, alkynyl, hydroxy, alkoxy, amino, alkylamino, or dialkylamino; and
  • R4 is optionally substituted with one or more substituents selected from hydrogen, alkyl, alkyenyl, alkynyl, hydroxy, alkoxy, amino, alkylamino, or dialkylamino.
  • Another object of the invention relates to a method for treating and/or preventing inflammatory bowel disease comprising administering a subject in need thereof with an agent capable of restoring the integrated stress response (ISR), as above described.
  • ISR integrated stress response
  • the agent capable of restoring the integrated stress response (ISR) may be administered in the form of a pharmaceutical composition, as defined below.
  • said inhibitor is administered in a therapeutically effective amount.
  • a “therapeutically effective amount” is meant a sufficient amount of the agent capable of restoring the integrated stress response (ISR) to treat and/or to prevent an inflammatory bowel disease at a reasonable benefit/risk ratio applicable to any medical treatment.
  • ISR integrated stress response
  • the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment.
  • the specific therapeutically effective dose level for any particular subject will depend upon a variety of factors including the disorder being treated and the severity of the disorder; activity of the specific compound employed; the specific composition employed, the age, body weight, general health, sex and diet of the subject; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific polypeptide employed; and like factors well known in the medical arts.
  • the daily dosage of the products may be varied over a wide range from 0.01 to 1,000 mg per adult per day.
  • the compositions contain 0.01, 0.05, 0.1, 0.5, 1.0, 2.5, 5.0, 10.0, 15.0, 25.0, 50.0, 100, 250 and 500 mg of the active ingredient for the symptomatic adjustment of the dosage to the subject to be treated.
  • a medicament typically contains from about 0.01 mg to about 500 mg of the active ingredient, preferably from 1 mg to about 100 mg of the active ingredient.
  • An effective amount of the drug is ordinarily supplied at a dosage level from 0.0002 mg/kg to about 20 mg/kg of body weight per day, especially from about 0.001 mg/kg to 7 mg/kg of body weight per day.
  • Another object of the invention relates to a method for treating an inflammatory bowel disease comprising:
  • step i) and ii) are performed concomitantly or preferably step ii) is performed sequentially after step i).
  • the agent capable of restoring the integrated stress response may be combined with pharmaceutically acceptable excipients, and optionally sustained-release matrices, such as biodegradable polymers, to form therapeutic compositions.
  • the active principle in the pharmaceutical compositions of the present invention for oral, sublingual, subcutaneous, intramuscular, intravenous, transdermal, local or rectal administration, can be administered in a unit administration form, as a mixture with conventional pharmaceutical supports, to animals and human beings.
  • Suitable unit administration forms comprise oral-route forms such as tablets, gel capsules, powders, granules and oral suspensions or solutions, sublingual and buccal administration forms, aerosols, implants, subcutaneous, transdermal, topical, intraperitoneal, intramuscular, intravenous, subdermal, transdermal, intrathecal and intranasal administration forms and rectal administration forms (suppository and enemas).
  • the pharmaceutical compositions contain vehicles which are pharmaceutically acceptable for a formulation capable of being injected.
  • vehicles which are pharmaceutically acceptable for a formulation capable of being injected.
  • These may be in particular isotonic, sterile, saline solutions (monosodium or disodium phosphate, sodium, potassium, calcium or magnesium chloride and the like or mixtures of such salts), or dry, especially freeze-dried compositions which upon addition, depending on the case, of sterilized water or physiological saline, permit the constitution of injectable solutions.
  • the pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions; formulations including sesame oil, peanut oil or aqueous propylene glycol; and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions.
  • the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms, such as bacteria and fungi.
  • Solutions comprising compounds of the invention as free base or pharmacologically acceptable salts can be prepared in water suitably mixed with a surfactant, such as hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.
  • the agent capable of restoring the integrated stress response (ISR) of the invention can be formulated into a composition in a neutral or salt form.
  • Pharmaceutically acceptable salts include the acid addition salts (formed with the free amino groups of the protein) and which are formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric, mandelic, and the like. Salts formed with the free carboxyl groups can also be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, histidine, procaine and the like.
  • the carrier can also be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), suitable mixtures thereof, and vegetables oils.
  • the proper fluidity can be maintained, for example, by the use of a coating, such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants.
  • the prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like.
  • isotonic agents for example, sugars or sodium chloride.
  • Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminium monostearate and gelatin.
  • Sterile injectable solutions are prepared by incorporating the active polypeptides in the required amount in the appropriate solvent with several of the other ingredients enumerated above, as required, followed by filtered sterilization.
  • dispersions are prepared by incorporating the various sterilized active ingredients into a sterile vehicle which contains the basic dispersion medium and the required other ingredients from those enumerated above.
  • the preferred methods of preparation are vacuum-drying and freeze-drying techniques which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
  • solutions Upon formulation, solutions will be administered in a manner compatible with the dosage formulation and in such amount as is therapeutically effective.
  • the formulations are easily administered in a variety of dosage forms, such as the type of injectable solutions described above, but drug release capsules and the like can also be employed.
  • aqueous solutions For parenteral administration in an aqueous solution, for example, the solution should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose.
  • aqueous solutions are especially suitable for intravenous, intramuscular, subcutaneous and intraperitoneal administration.
  • sterile aqueous media which can be employed will be known to those of skill in the art in light of the present disclosure.
  • one dosage could be dissolved in 1 ml of isotonic NaCl solution and either added to 1000 ml of hypodermoclysis fluid or injected at the proposed site of infusion. Some variation in dosage will necessarily occur depending on the condition of the subject being treated. The person responsible for administration will, in any event, determine the appropriate dose for the individual subject.
  • the agent capable of restoring the integrated stress response (ISR) of the invention may be formulated within a therapeutic mixture to comprise about 0.0001 to 1.0 milligrams, or about 0.001 to 0.1 milligrams, or about 0.1 to 1.0 or even about 10 milligrams per dose or so. Multiple doses can also be administered.
  • parenteral administration such as intravenous or intramuscular injection
  • other pharmaceutically acceptable forms include, e.g. tablets or other solids for oral administration; liposomal formulations; time release capsules ; and any other form currently used.
  • Biopsies (5 to 10) were taken from the right and left colon of each healthy control subject and processed as above. Endoscopic findings were normal in all subjects and biopsy specimens were systematically diagnosed as nonsignificant abnormalities with HE staining. Surgical samples were taken from healthy colon mucosa at least 10 cm from the cancer site, and processed as above.
  • Biopsies were routinely stained with HE. Histological assessment of mucosal damage and inflammatory cell infiltrate were graded by the same expert pathologist using a previously validated score to characterize colonic involvement of IBD 12 .
  • Immunohistological methods were performed on serial 4 ⁇ m deparaffinised sections from control and unaffected UC mucosa. After endogenous peroxidase removal, sections were incubated with specific antibodies. For immunofluorescence studies, cryoslide sections from control and unaffected UC mucosa were incubated with anti-TIA-1 and anti-eIF3 antibodies, and then labeled with secondary antibodies. Nuclei were stained using DAPI or TO-PRO-3 iodide. Fluorescence was detected by confocal laser scanning microscopy (CLSM-510-META, Zeiss). All images were acquired by using the Zeiss LSM Image Browser software.
  • Colon biopsies were homogenized as previously described 13 . Equal amounts of total protein (50-75 ⁇ g) were then subjected to SDS-PAGE, transferred using iBlotGel Transfer Device (Invitrogen), and probed with primary antibodies (see Supplemental Informations). Labeled protein bands were scanned with an HP Scanjet 5500, and the relative protein content was determined by densitometric analysis.
  • RNA quality was determined with an Agilent 2100 Bioanalyzer, as well as in a control gradient in which pooled colonic tissue from 2 UC patients and 2 healthy subjects were lysed in the presence of 25 mM EDTA (pH 8.0).
  • the resulting polysome profile demonstrated a complete shift in mRNAs from the polysome-bound fraction towards lighter sucrose fractions (data not shown) verifying proper identification of polysomal peaks.
  • RNAs from polysomes were pooled and purified. RNA samples were amplified and Cy3 labeled following the manufacturer's protocol.
  • the hybridization procedure was performed by Tebu-Bio according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit and Agilent Whole Human Genome Oligo Microarrays 4 ⁇ 44 K which covers more than 41,000 genes and transcripts. Normalized data were serially filtered in the following order: eliminate genes flagged as absent in all groups, select genes up- or downregulated by at least two fold with P ⁇ 0.05 (unaffected UC mucosa vs. control, Student t-test with Benjamini and Hochberg false discovery rate as multiple testing correction).
  • Unaffected colonic UC mucosa exhibit extended IRE1 ⁇ and ATF6 ⁇ branch signaling
  • UPR activation was assessed by determining IRE1 ⁇ -mediated splicing of XPB-1 in unaffected colonic mucosa from UC and healthy individuals.
  • Spliced XBP-1 (XBP-1s) mRNA levels were significantly increased in UC mucosa and the ratio of spliced to unspliced XBP-1 (XBP-1s/XBP-1u) was 1.8 and 3.8 in controls and UC, respectively.
  • Consistent with hyperactivation of the IRE1 ⁇ /XBP-1 arm increased expression of the UPR-related target genes GRP94, GRP78, and EDEM1 was observed in UC patients compared to controls.
  • IRE1 can also activate the c-Jun-N-terminal kinases (JNK) which regulate apoptosis and/or proinflammatory gene expression through the TRAF2/MAP3K cascade 15 .
  • JNK c-Jun-N-terminal kinases
  • ATF6 ⁇ functions as a proximal inducer of the UPR as p5OATF6 ⁇ , the active bZIP transcription factor converted from the latent p9OATF6 ⁇ , binds ER stress-responsive elements of genes including GRP78, GRP94 and XBP-1u 9 .
  • the generation of p50ATF6 ⁇ was reproducible in UC patients and was associated with enhanced XBP-1u mRNA levels compared to controls.
  • SGs stress granules
  • SGs are specialized cytoplasmic foci which regulate mRNA translation and form by both eIF2 ⁇ phosphorylation-dependent 18 and -independent mechanisms 19 .
  • SGs contain stalled mRNA bound to small 40S ribosomal subunits, aggregation-prone ribonucleoproteins (e.g. TIA-1 and TIAR), and different translational initiation factors 18 .
  • Cryoslide sections of unaffected UC and control colonic mucosa were immunostained for TIA-1 and eIF3, two components of SGs. Colocalization of TIA-1 and eIF3 was observed in abundant punctuate foci in control colonic epithelial cells. In contrast, a marked loss of SGs was observed in unaffected UC mucosa. Only 10% of epithelial cells from each UC tissue section contained at least 5 SGs compared to over 45% of control mucosal cells. The reduction in the number of SGs was not due to a decrease in TIA-1 or eIF3 protein expression levels since Western blot analysis showed increased expression of these two proteins in unaffected UC mucosa.
  • Altered ER stress response entails post-transcriptional reprogramming of mRNA translation in unaffected UC mucosa
  • eIF4E a key player in translation initiation is eIF4E which is the limiting component of the eIF4F initiation complex.
  • This complex contains two other subunits: eIF4A (an ATP-dependent helicase) and eIF4G (a large scaffolding protein), which associate with eIF4E and play a crucial role in the eIF2 ⁇ phosphorylation-independent mechanisms of SG formation 19 .
  • eIF4E expression level was ⁇ 35% higher in unaffected UC mucosa than in controls.
  • eIF4E In addition to its level of expression, availability of eIF4E for translation initiation is controlled by the phosphorylation status of 4E-BP 1 20 .
  • the level of hyperphosphorylated 4E-BP1 (Ser65) was found to be increased by about threefold while the level of total 4E-BP1 was not affected in unaffected UC mucosa compared to controls.
  • eIF4E phosphorylation (Ser209) which stimulates translation efficiency 21 and eIF4A and eIF4G expression levels were significantly increased in unaffected UC mucosa suggesting that eIF4F formation and unwinding of RNA secondary structures are optimized in unaffected UC mucosa.
  • Gene products were grouped into functional categories and molecular functions according to the Gene Ontology (http://www.geneontology.org), GeneCards (http://www.genecards.org/) and GeneNote (http://bioinfo2.weizmann.ac.il/cgibin/genenote) data bases.
  • GeneOntology http://www.geneontology.org
  • GeneCards http://www.genecards.org/
  • GeneNote http://bioinfo2.weizmann.ac.il/cgibin/genenote
  • genes coding for ER stress response ATF4, ATF6, ER01
  • translation elF2alpha, eIF5A
  • mucins MUC2, MUC4, MUC12, MUC20
  • ZO1, keratins, claudins immune response and antibacterial defenses
  • TLR4, TLR6, IFR5, interleukins detoxification and antioxidative stress
  • TST/CES2, SOD1, SOD2 detoxification and antioxidative stress
  • wound repair and cell cycle cdc42, p21 WAF/cip , HMGB1 indicating that our microarray analysis provided an accurate representation of gene expression in unaffected UC mucosa and revealed new altered biological functions in UC.
  • CDKN1A p21 WAF/cip
  • eIF5A which promotes translation elongation, polysome disassembly, SG assembly, and inflammation 25
  • RanBP2 which functions as the small ubiquitin-related E3 ligase of TCF-4 enhancing the Wnt signaling pathway 26
  • SOD1 superoxide dismutase 1
  • PgR polymeric immunoglobulin receptor
  • TST/CES2 thiosulfate sulfurtransferase
  • p21 WAF/cip , eIF5A, SOD1, PIgR, and TST/CES2 protein expression levels were significantly decreased while RanBP2 protein levels were increased in unaffected UC vs. control mucosa.
  • Protein level changes in PIgR (membrane-associated protein) and TST/CES2 (mitochondrion-associated protein) were confirmed by immunohistochemistry (FIG. 5B).
  • ISR immunosensing protein
  • Decreased TST/CES2 expression in unaffected UC mucosa may increase epithelial cell exposure to the toxic effects of H 2 5 and lead to or worsen UC, as well as promote colorectal carcinogenesis 29 .
  • the downregulation of critical cytoprotective proteins through the reprogramming of mRNA translation in UC could impair epithelial homeostasis.
  • This could be the first step towards identifying the regulatory networks that control translational repression whose dysregulation could be involved in the pathophysiology of UC.
  • RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly.Nat Cell Biol 2008;10:1224-1231
  • Ulcerative colitis is characterized by exclusive colonic involvement with superficial mucosal lesions associated with depletion in goblet cells and decreased secretion of mucins in inflammatory colonic mucosa 11 .
  • epithelium of UC patients is diffusely abnormal irrespective to inflammation 12
  • early alterations predating inflammation within colonic epithelial cell remain elusive.
  • impairment of proper ERS resolution by altered unfolded protein response (UPR) in epithelial cells can lead or sensitize to colonic inflammation both in animal 2-8 and human studies 1,2 .
  • URR unfolded protein response
  • the UPR is a carefully orchestrated process involving three proximal sensors PERK, ATF6, and IRE1 that allow cells to cope with a wide variety of stressful conditions.
  • the combined action of these sensors restores cell homeostasis by cessation of protein translation, increase of chaperones production, and degradation of the burden of aberrant proteins.
  • Sustained or abnormal ERS adversely affects normal cell function leading to inflammation and/or apoptosis 13,14 .
  • goblet cells goblet cells and mucus barrier have been linked to inflammation.
  • knockout of the mucin gene Muc2 in mice is not sufficient to cause colitis since inflammation appears to arise only on a permissive genetic background 15,16 and patients with UC express MUC2.
  • partial or total depletion in the number of goblet cells 17,20 and therefore in mucus and antibacterial products is insufficient to induce colitis.
  • accumulation of missense mutated Muc2 ref7 or HLA-27 protein 9 which is prone to misfolding in the ER, or knockout of the protein disulfide isomerase Agr2 ref.5 induce exaggerated ERS in secretory cells and subsequent inflammation.
  • the predisposition to colitis might reside in goblet cells themselves and in their inability to manage ERS in the absence of immune dysfunction and in the setting of a normal colonic flora.
  • goblet cells are affected by ERS in UC
  • IL-10 KO mice which express deregulated ERS in epithelial intestinal cells 8 and develop enterocolitis depending on both genetic background and environmental factors 21 .
  • mice We next measured epithelial permeability of FITC-dextran in segments of distal colon and indigenous bacterial translocation was identified in the spleen of 7 and 12-week-old mice. Consistent with the colitis state, only IL10/Nox1 dKO mice demonstrated an increased permeability in the colon and exhibited splenomegaly which was closely correlated with increased Gram-negative commensal bacteria translocation. Interestingly, IL10/Nox1 dKO mice showed the main complications of UC such as colitis-associated colorectal cancer and spontaneously primary sclerosing cholangitis at 7/8 months of age.
  • IL10 KO mice showed mild enterocolitis at low frequency ( ⁇ 20% at 34 weeks), without showing any signs of cholangitis or colorectal cancer (at >8 months of age; this study and 29 ).
  • IL10 KO mice showed mild enterocolitis at low frequency ( ⁇ 20% at 34 weeks), without showing any signs of cholangitis or colorectal cancer (at >8 months of age; this study and 29 ).
  • IL10 KO mice showed mild enterocolitis at low frequency ( ⁇ 20% at 34 weeks), without showing any signs of cholangitis or colorectal cancer (at >8 months of age; this study and 29 ).
  • IL10 10/ Nox1 dKO mice 6- and 16-wk-old mice. Consistent with previous findings in patients with UC 30,31 , IL10/Nox1 dKO mice expressed almost 50% of microRNAs relevant in defining UC signature.
  • IL10/Nox1 dKO mice showed increased expression levels of pro-inflammatory cytokines mainly involved in UC. Lymphoid and myeloid cell population analysis in the spleen did not differ between the four genotypes. By contrast, a massive infiltration of FoxP3 + T reg was only observed in the colonic tissue in spite of active mucosal inflammation and at lesser extent in the spleen of IL10/Nox1 dKO mice consistent with findings in UC 32 .
  • BM bone marrow
  • the colonic epithelium of IL10/Nox1 dKO mice showed a paucity of Alcian Blue/PAS positive mucins associated with loss of goblet cells at the ulcerated sites. Accordingly, Muc2 and Muc4 protein expression levels were dramatically low in the inflamed colonic areas of IL10/Nox1 dKO mice. Rarefaction and aberrant morphology of goblet cells with few and immature thecae associated with small amount of mucus and swollen, round mitochondria were similarly observed in the colon of both IL10/Nox1 dKO mice and patients with UC.
  • Colonic section of IL10/Nox1 dKO mice displayed an increase in the number of PCNA-and phospho-histone 3-positive cells suggesting increased epithelial proliferation.
  • Scanning electron microscopy (SEM) showed a ⁇ 30% increase in crypt length in IL10/Nox1 dKO mice.
  • SEM displayed a wide spectrum of identical ultrastructural alterations of the mucosa both in IL10/Nox1 dKO mice and in unaffected colonic mucosa of patients with UC including crypt distortion, visible crypt openings disposed in rows, edematous glandular borders, and dilatation of the gland lumen.
  • staining and quantitative assessment of apoptotic cells indicated that decreased expression of goblet cells in IL10/Nox1 dKO mice was due to increased apoptosis in the colon.
  • IL10/Nox1 dKO mice exhibited ERS disturbances in the colonic mucosa prior inflammatory damages as previously described in patients with UC 1 .
  • IRE1beta and ATF6alpha branch signaling were extended in colonic epithelial cells as evidenced by the increased XBP-1 mRNA splicing, the induction of GRP78, GRP94, PDI at both mRNA and protein levels, and dilated cisternae and gross distortion of the ER in goblet cells.
  • identical defective phosphorylation of eIF2 ⁇ correlated with low expression of ATF4 was observed both in unaffected colonic mucosa of IL10/Nox1 dKO mice and patients with UC 1 .
  • EIF2alpha phosphorylation is cytoprotective during ERS, because cells are sensitized to cell death when this pathway is genetically ablated 33 and protected when it is ectopically enforced 34 .
  • IL10/Nox1 dKO mice were treated with 1 mg/kg salubrinal 35 for up to three weeks.
  • salubrinal strongly reduced histological colitis score throughout the colon, markedly prevented immune cell infiltration, and restored intact mucosal architecture with normal goblet cells.
  • Salubrinal caused robust elF2alpha phosphorylation and protected colonic mucosa against apoptosis at least in part for its anti-apoptotic activity on CHOP expression.
  • NADPH oxidase 1 modulates WNT and NOTCH1 signaling to control the fate of proliferative progenitor cells in the colon. Mol Cell Biol 30, 2636-2650 (2010).

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