US20100016783A1 - Non-invasive systems and methods for in-situ photobiomodulation - Google Patents

Non-invasive systems and methods for in-situ photobiomodulation Download PDF

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US20100016783A1
US20100016783A1 US12/417,779 US41777909A US2010016783A1 US 20100016783 A1 US20100016783 A1 US 20100016783A1 US 41777909 A US41777909 A US 41777909A US 2010016783 A1 US2010016783 A1 US 2010016783A1
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agent
energy
plasmonics
target structure
active agent
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Frederic J. BOURKE, JR.
Tuan Vo-Dinh
Harold Walder
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Immunolight LLC
Duke University
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Immunolight LLC
Duke University
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Priority to US12/417,779 priority Critical patent/US20100016783A1/en
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Assigned to DUKE UNIVERSITY, IMMUNOLIGHT LLC reassignment DUKE UNIVERSITY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: VO-DINH, TUAN, WALDER, HAROLD, BOURKE, FREDERIC A., JR.
Publication of US20100016783A1 publication Critical patent/US20100016783A1/en
Priority to US12/764,184 priority patent/US9302116B2/en
Priority to US12/843,188 priority patent/US9662389B2/en
Priority to US12/943,787 priority patent/US9232618B2/en
Priority to US14/168,795 priority patent/US9526913B2/en
Priority to US14/603,539 priority patent/US9439897B2/en
Priority to US15/126,834 priority patent/US10087343B2/en
Priority to US14/716,394 priority patent/US9526914B2/en
Priority to US15/322,928 priority patent/US10410991B2/en
Priority to US15/045,524 priority patent/US10493296B2/en
Priority to US15/151,642 priority patent/US10391330B2/en
Priority to US15/183,110 priority patent/US9676918B2/en
Priority to US15/220,596 priority patent/US20160331731A1/en
Priority to US15/247,367 priority patent/US10384071B2/en
Priority to US15/649,956 priority patent/US9993661B2/en
Priority to US15/874,426 priority patent/US10272262B2/en
Priority to US16/511,605 priority patent/US20190336786A1/en
Priority to US17/931,105 priority patent/US20230029054A1/en
Abandoned legal-status Critical Current

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Definitions

  • the present invention relates to methods and systems for treating a disorder or condition in a subject, that provide better distinction between normal, healthy cells and those cells suffering the disorder or condition (hereafter “target cells”) and preferably that can be performed using non-invasive or minimally invasive techniques.
  • Photobiomodulation also known as low level laser therapy (LLLT), cold laser therapy, and laser biostimulation, is an emerging medical and veterinary technique in which exposure to low-level laser light can stimulate or inhibit cellular function leading to beneficial clinical effects.
  • LLLT low level laser therapy
  • the “best” combination of wavelength, intensity, duration and treatment interval is complex and sometimes controversial with different diseases, injuries and dysfunctions needing different treatment parameters and techniques.
  • Certain wavelengths of light at certain intensities will, for example, aid tissue regeneration, resolve inflammation, relieve pain and boost the immune system.
  • the exact mechanism is still being explored and debated but it is agreed that the mechanism is photochemical rather than heat-related. Observed biological and physiological effects include changes in cell membrane permeability, and up-regulation and down-regulation of adenosine triphosphate and nitric oxide.
  • All light-induced biological effects depend on the parameters of the irradiation (wavelength, dose, intensity, irradiation time, depth of a target cell, and continuous wave or pulsed mode, pulse parameters).
  • Laser average power is typically in the range of 1-500 mW; some high peak power, short pulse width devices are in the range of 1-100 W with typically 200 ns pulse widths.
  • the average beam irradiance then is typically 10 mW/cm 2 -5 W/cm 2 .
  • the wavelength is typically in the range 600-1000 nm.
  • the red-to-near infrared (NIR) region is preferred for photobiomodulation.
  • Other wavelengths may be also used, e.g., UV light for neurons and green light for prostate tissue.
  • Maximum biological responses are occurring when irradiated at 620, 680, 760, and 820-830 nm (Karu T I, et al., (1998). The Science of Low Power Laser Therapy. Gordon and Breach Sci. Publ., London). Large volumes and relatively deeper layers of tissues can be successfully irradiated by laser only (e.g., inner and middle ear diseases, injured siatic or optical nerves, inflammations).
  • the LEDs are used for irradiation of surface injuries.
  • a photoacceptor must first absorb the light used for the irradiation. After promotion of electronically excited states, primary molecule processes from these states can lead to a measurable biological effect (via secondary biochemical reaction, or photosignal transduction cascade, or cellular signaling) at the cellular level.
  • a photoacceptor for eukaryotic cells in red-to-NIR region is believed to be the terminal enzyme of the respiratory chain cytochrome c oxidase located in cell mitochondrion. In the violet-to blue spectra region, flavoprotein (e.g., NADHdehydrogenase in the beginning of the respiratory chain) is also among the photoacceptors.
  • Clinical applications of photobiomodulation include, for example, treating soft tissue and bone injuries, chronic pain, wound healing, nerve regeneration, sensory regeneration/restoration and possibly even resolving viral and bacterial infections, treating neurological and phychiatric diseases (e.g., epilepsy and Parkinson's disease) (e.g., Zhang F., et al., Nature, 446:617-9 (Apr. 5, 2007; Han X., et al., PloS ONE, 2(3):e299 (Mar. 21, 2007); Arany P R, et al., Wound Repair Regen., 15(6):866-74 (2007); Lopes C B, et al., Photomed. Laser Surg., 25(2):96-101 (2007)).
  • neurological and phychiatric diseases e.g., epilepsy and Parkinson's disease
  • Zhang F., et al., Nature, 446:617-9 Apr. 5, 2007; Han X., et al., PloS ONE, 2
  • An NIR light treatment can prevent cell death (apoptosis) in cultured neurons (brain) cells (Wong-Reiley M T, et al., JBC, 280(6):4761-71 (2005)). Specific wavelengths of light can promote cellular proliferation to the activation of mitochondria, the energy-producing organelles within the cell via cytochrome c oxidase.
  • An NIR treatment can augment mitochondrial function and stimulate antioxidant protective pathways. The evidence that the NIR treatment can augment mitochondrial function and stimulate antioxidant protective pathways comes from photobiomodulation experiments carried out using a laboratory model of Parkinson's disease (PD) (cultures of human dopaminergic neuronal cells) (Whelan H., et. al., SPIE, Newsroom, pages 1-3 (2008)).
  • PD Parkinson's disease
  • the excitable cells e.g., neurons, cardiomyocites
  • the photoacceptors are also believed to be components of respiratory chain. It is clear from experimental data (Karu, T. I., (2002). Low-power laser therapy. In: CRC Biomedical Photonics Handbook, T. Vo-Dinh, Editor-in-Chief, CRC Press, Boca Raton (USA)) that irradiation can cause physiological and morphological changes in nonpigmental excitable cells via absorption in mitochondria. Later, similar irradiation experiments were performed with neurons in connection with low-power laser therapy.
  • Photobiological action via activation of respiratory chain is believed to be a general mechanism occurring in cells.
  • Crucial events of this type of cell metabolism activation are occurring due to a shift of cellular redox potential into more oxidized direction as well as due to ATP extrasynthesis.
  • Susceptibility to irradiation and capability for activation depend on physiological status of irradiated cells: the cells, which overall redox potential is shifted to more reduced state (example: some pathological conditions) are more sensitive to the irradiation.
  • the specificity of final photobiological response is determined not at the level of primary reactions in the respiratory chain but at the transcription level during cellular signaling cascades. In some cells, only partial activation of cell metabolism happens by this mechanism (example: redox priming of lymphocytes).
  • Red-to-NIR radiation has been shown to promote wound healing, e.g., infected, ischemic, and hypoxic wounds (Wong-Reley, W T T, JBC, 280(6):4761-4771 (2005)). Red-to-NIR radiation also protects the retina against the toxic actions of methanol-derived formic acid in a rodent model of methanol toxicity and may enhance recovery from retinal injury and other ocular diseases in which mitochondrial dysfunction is postulated to play a role (Eells J T., PNAS, 100(6):3439-44 (2003)). Other clinical applications of photobiomodulation is repair of soft and bone tissues by IR laser irradiation (Martinez M E, et al., Laser in Med. Sci., 2007).
  • Invasive laser assisted liposuction is a recently developed method, wherein a laser fiber is introduced through a tube into the skin and directly to the fat cells causing the cells to rapture and drain away as liquid (Kim K H, Dermatol. Surg., 32(2):241-48 (2006)). Tissue around the area is coagulated.
  • another application of photobiomodulation is a non-surgical varicose vein treatment (an endovenous laser therapy), wherein a laser is threaded through an incision and the full length of the varicose vein (Kim H S, J. Vasc. Interv. Radiol., 18(6):811 (2007)). When the laser is slowly withdrawn, heat is applied to the vein walls, causing the vein to permanently close and disappear.
  • the green light laser is a laser that vaporizes and removes the enlarged prostate tissue (Heinrich E., Eur. Urol., 52(6):1632-7 (2007)).
  • the significance of the color of the laser light (green) is that this results in absorption by hemoglobin which is contained within red blood cells and not absorbed by water.
  • the procedure may also be known as laser prostatectomy or laser Transurethral resection of the prostate (TURP).
  • the technique involves painting the enlarged prostate with the laser until the capsule of the prostate is reached. By relieving this portion of the prostate, patients are able to void much easier through a wide-open channel in the prostate.
  • the procedure needs to be performed under general or spinal anesthesia.
  • An advantage of the procedure is that even patients taking blood thinners (e.g., aspirin to prevent stroke) can be treated because there is less bleeding compared to a traditional surgery.
  • photobiomodulation is a direct control of brain cell activity with light.
  • the technique is bas(d upon NIR spectroscopy and is simpler to use and less expensive than other methods such as functional magnetic resonance imaging and positron emission tomography.
  • hemoglobin oxygen concentrations in the brain obtained simultaneously by NIR spectroscopy and by functional MRI, the current “gold standard” in brain studies, was compared. Both methods were used to generate functional maps of the brain's motor cortex during a periodic sequence of stimulation by finger motion and rest. Spatial congruence between the hemoglobin signal and the MRI signal in the motor cortex related to finger movement was demonstrated. The researchers also demonstrated collocation between hemoglobin oxygen levels and changes in scattering due to brain activities. The changes in scattering associated with fast neuron signals came from exactly the same locations.
  • a low-intensity laser light-oxygen cancer therapy is another application of photobiomodulation.
  • the light-oxygen effect (LCE), which involves activation of or damage to biosystems by optical radiation at low optical doses by direct photoexcitation of molecular oxygen dissolved in a biosystem so that it is converted to the singlet state, i.e., by photogeneration of molecular singlet oxygen from O 2 dissolved in cells, similar to photodynamic effect (Zakharov S D, et al., Quantum Electronics, 29(12):1031-53 (1999)). It was shown that the He-Ne laser radiation destroys tumor cells in the presence or absence of the photosensitiser.
  • the LOE can be activated by small optical doses, which are 4-5 orders of magnitude lower that those found if a comparison is made with the familiar analogue in the form of the photodynamic effect (PDE).
  • This type of photobiomodulation methods fall into two general categories: one set of methods uses light to uncage a compound that then becomes biochemically active, binding to a downstream effector. For example, this method involves applying “caged” chemicals to a sample and then using light to open the cage to invoke a reaction. Modified glutamate is useful for finding excitatory connections between neurons, since the uncaged glutamate mimics the natural synaptic activity of one neuron impinging upon another. This method is used for elucidation of neuron functions and imaging in brain slices using, for example, two-photon glutamine uncageing (Harvey C D, et al., Nature, 450:1195-1202 (2007); Eder M, et al., Rev.
  • UV light stimulation e.g., GABA
  • secondary messengers e.g., Ca 2+ and Mg 2+
  • carbachol e.g., achol
  • capsaicin e.g., ATP
  • the other major photostimulation method is the use of light to activate a light-sensitive protein such as rhodopsin (ChR2), which can then excite the cell expressing the opsin.
  • ChR2 rhodopsin
  • channelrhodopsin-2 a monolithic protein containing a light sensor and a cation channel, provides electrical stimulation of appropriate speed and magnitude to activate neuronal spike firing.
  • photoinhibition the inhibition of neural activity with light, has become feasible with the application of molecules such as the light-activated chloride pump halorhodopsin to neural control.
  • blue-light activated channelrhodopsin-2 and the yellow light-activated chloride pump halorhodopsin enable multiple-color, optical activation and silencing of neural activity.
  • ChR2 photostimulaiton involves genetic targeting ChR2 to neurons and light pulsing the neurons expressing ChR2 protein.
  • the experiments have been conducted in vitro and in vivo in mice by in vivo deep-brain photostimulaiton using optical fibers to deliver light into the lateral hypothalamus (Adamantidis A R, et al., Nature 450:420-425 (2007)).
  • Genetic targeting of ChR2 allows exclusive stimulation of defined cellular subsets and avoids the need for addition of the caged glutamate, facilitating photostimulation in vivo (Wang H., et al., PNAS, 104(19):8143-48 (2007)).
  • ChR2 photostimulation has been used for restoring visual activity in mice with impaired vision, to evoke behavioral responses in worms and flies (Wang H., et al., 2007).
  • the robust associative learning induced by ChR2-assisted photostimulaiton in mice opens the door to study the circuit basis of perception and cognition in vivo (Huber D., et al., 2007).
  • This kind of neuronal targeting and stimulation might have clinical application, e.g., deep brain stimulation to treat Parkinson's disease and other disorders, controlling behavioral, perceptional and cognitive characteristics, and for imaging and studying how the brain works (Zhang F., et al., Nature Methods, 3(10):785-792 (2006); Wong-Riley M T., et al., JBC, 280(6):4761-4771 (2005)).
  • NpHR chloride pump
  • Light-sensitive proteins can be introduced into cells or live subjects via a number of techniques including electroporation, DNA microinjection, viral delivery, liposomal transfection and calcium-phosphate precipitation.
  • a third photostimulation technique is chemical modification of ion channels and receptors to render them light-responsive.
  • Some of the most fundamental signaling mechanisms in a cell involve the release and uptake of Ca 2+ ions.
  • Ca 2+ is involved in controlling fertilization, differentiation, proliferation, apoptosis, synaptic plasticity, memory, and developing axons.
  • Ca2+ waves can be induced by UV irradiation (single-photon absorption) and NIR irradiation (two-photon absorption) by releasing caged Ca 2+ , an extracellular purinergic messenger InsP3 (Braet K., et al., Cell Calcium, 33:37-48 (2C03)), or ion channel ligands (Zhang F., et al., 2006).
  • Directly controlling a brain cell activity with light is a novel means for experimenting with neural circuits and could lead to therapies for some disorders. This accomplishment is a step toward the goal of mapping neural circuit dynamics on a millisecond timescale to see if impairments in these dynamics underlie severe psychiatric symptoms. Knowing the effects that different neurons have could ultimately help researchers figure out the workings of healthy and unhealthy brain circuits. If use of the technique can show that altered activity in a particular kind of neuron underlies symptoms, for example, this insight will allow development of targeted genetic or pharmaceutical treatments to fix those neurons. Conceivably, direct control of neuronal activity with light could someday become a therapy in itself.
  • neural communications Another potential application is experimenting with simulating neural communications. Because neurons communicate by generating patterns of signals-sometimes on and sometimes off like the 0s and 1s of binary computer code-flashing blue and yellow lights in these patterns could compel neurons to emit messages that correspond to real neural instructions. In the future, this could allow researchers to test and tune sophisticated neuron behaviors. Much farther down the road, the ability to artificially stimulate neural signals, such as movement instructions, could allow doctors to bridge blockages in damaged spinal columns, perhaps restoring some function to the limbs of paralyzed patients.
  • the laser systems currently used for biostimulation do not allow performing photobiomodulation in a region deep within thick tissue without a surgical invasion.
  • Laser therapy is mostly conducted in surface or near surface target cells and tissue because penetration of UV and red-to-N IR radiation used for photobiomodulation and photobiostimulaiton is no more than a few centimeters beneath the surface of the skin.
  • imaging and stimulation of brain cells is mainly possible in thin brain slices, or a thin monolayer or suspension of cells.
  • a subject undergoes various invasive surgical procedures, e.g., invasive insertion of a fiber via incisions into a fat layer or veins, implanting a radiation source in deep tissue, or implanting a glass window above the barrel cortex (Huber D., et al., Nature, 451:61-66 (2007)). It is further well recognized that another problem associated with the existing methods of photobiomodulation is in differentiation of normal cells from target cells.
  • Photodynamic therapy is a treatment modality that uses a photosensitizing agent and laser light to kill cells.
  • PDT is a relatively new light-based treatment, which has recently been approved by the United States Food & Drug Administration (FDA) for the treatment of both early and late-stage lung cancer. Other countries have approved PDT for treatment of various cancers as well.
  • FDA United States Food & Drug Administration
  • PDT is useful in treating all cell types, whether small cell or non-small cell carcinoma.
  • PDT involves treatment of diseases such as cancer using light action on a special photoactive class of drugs, by photodynamic action in vivo to destroy or modify tissue [Dougherty T. J. and Levy J.
  • PDT Photodynamic Therapy and Clinical Applications
  • pre-cancerous conditions e.g. actinic keratoses
  • high-grade dysplasia in Barrett's esophagus e.g. various eye diseases, e.g. age related macular degeneration (AMD).
  • AMD age related macular degeneration
  • Photodynamic therapy (PDT) is approved for commercialization worldwide both for various cancers (lung, esophagus) and for AMD.
  • the PDT process requires three elements: (1) a PA drug (i.e., photosensitizer), (2) light that can excite the photosensitizer and (3) endogenous oxygen.
  • the putative cytotoxic agent is singlet oxygen, an electronically excited state of ground state triplet oxygen formed according to the Type II photochemical process, as follows.
  • the triplet state has a relatively long lifetime ( ⁇ sec to seconds) only photosensitizers that undergo efficient intersystem crossing to the excited triplet state will have sufficient time for collision with oxygen in order to produce singlet oxygen.
  • the energy difference between ground state and singlet oxygen is 94.2 kJ/mol and corresponds to a transition in the near-infrared at ⁇ 1270 nm.
  • Most PA photosensitizers in clinical use have triplet quantum yields in the range of 40-60% with the singlet oxygen yield being slightly lower. Competing processes include loss of energy by deactivation to ground state by fluorescence or internal conversion (loss of energy to the environment or surrounding medium).
  • PDT drug activity can become an issue if high doses of photosensitizer are necessary in order to obtain a complete response to treatment.
  • An important mechanism associated with PDT drug activity involves apoptosis in cells.
  • the photosensitiser Upon absorption of light, the photosensitiser (PS) initiates chemical reactions that lead to the direct or indirect production of cytotoxic species such as radicals and singlet oxygen.
  • the reaction of the cytotoxic species with subcellular organelles and macromolecules (proteins, DNA, etc) lead to apoptosis and/or necrosis of the cells hosting the PDT drug.
  • the preferential accumulation of PDT drug molecules in cancer cells combined with the localized delivery of light to the tumor results in the selective destruction of the cancerous lesion.
  • PDT does not involve generalized destruction of healthy cells.
  • PDT can also act on the vasculature, reducing blood flow to the tumor causing its necrosis. In particular cases it can be used as a less invasive alternative to surgery.
  • porphyrin-based sensitizers There are several chemical species used for PDT including porphyrin-based sensitizers.
  • a purified hematoporphyrin derivative, Photofrin® has received approval of the US Food and Drug Administration.
  • Porphyrins are generally used for tumors on or just under the skin or on the lining of internal organs or cavities because theses drug molecules absorbs light shorter than 640 nm in wavelength.
  • second generation sensitizers which have absorbance in the NIR region, such as porphyrin-based systems [R. K.
  • PDT retains several photosensitizers in tumors for a longer time than in normal tissues, thus offering potential improvement in treatment selectivity.
  • Corner C. “Determination of [3H]- and [14C] hematoporphyrin derivative distribution in malignant and normal tissue,” Cancer Res 1979, 3 9: 146-151; Young S W, et al., “Lutetium texaphyrin (PCI-0123) a near-infrared, water-soluble photosensitizer,” Photochem Photobiol 1996, 63:892-897; and Berenbaum M C, et al., “Meso-Tetra(hydroxyphenyl)porphyrins, a new class of potent tumor photosensitisers with favorable selectivity,” Br J Cancer 1986, 54:717-725.
  • Photodynamic therapy uses light of a specific wavelength to activate the photosensitizing agent.
  • Various light sources have been developed for PDT, which include dye lasers and diode lasers. Light generated by lasers can be coupled to optical fibers that allow the light to be transmitted to the desired site. See Pass 1-11, “Photodynamic therapy in oncology: mechanisms and clinical use,” J Natl Cancer Inst 1993, 85:443-456. According to researchers, the cytotoxic effect of PDT is the result of photooxidation reactions, as disclosed in Foote C S, “Mechanisms of photooxygenation,” Proa Clin Biol Res 1984, 170:3-18. Light causes excitation of the photosensitizer, in the presence of oxygen, to produce various toxic species, such as singlet oxygen and hydroxyl radicals. It is not clear that direct damage to DNA is a major effect; therefore, this may indicate that photoactivation of DNA crosslinking is not stimulated efficiently.
  • PDT retains several photosensitizers in tumors for a longer time than in normal tissues, thus offering potential improvement in treatment selectivity.
  • Corner C. “Determination of [3H]- and [14C] heinatoporphyrin derivative distribution in malignant and normal tissue,” Cancer Res 1979, 3 9: 146-151; Young S W, et al., “Lutetium texaphyrin (PCI-0123) a near-infrared, water-soluble photosensitizer,” Photochem Photobiol 1996, 63:892-897; and Berenbaum M C, et al., “Meso-Tetra(hydroxyphenyl)porphyrins, a new class of potent tumor photosensitisers with favorable selectivity,” Br J Cancer 1986, 54:717-725.
  • Photodynamic therapy uses light of a specific wavelength to activate the photosensitizing agent.
  • Various light sources have been developed for PDT that include dye lasers and diode lasers. Light generated by lasers can be coupled to optical fibers that allow the light to be transmitted to the desired site. See Pass 1-11, “Photodynamic therapy in oncology: mechanisms and clinical use,” J Natl Cancer Inst 1993, 85:443-456. According to researchers, the cytotoxic effect of PDT is the result of photooxidation reactions, as disclosed in Foote C S, “Mechanisms of photooxygenation,” Proa Clin Biol Res 1984, 170:3-18. Light causes excitation of the photosensitizer, in the presence of oxygen, to produce various toxic species, such as singlet oxygen and hydroxyl radicals. It is not clear that direct damage to DNA is a major effect; therefore, this may indicate that photoactivation of DNA crosslinking is not stimulated efficiently.
  • Photopheresis has been successfully used for treatment of cell proliferation disorders.
  • Exemplary cell proliferation disorders may include, but are not limited to, cancer, bacterial infection, immune rejection response of organ transplant, solid tumors, viral infection, autoimmune disorders (such as arthritis, lupus, inflammatory bowel disease, Sjogrens syndrome, multiple sclerosis) or a combination thereof, as well as aplastic conditions wherein cell proliferation is low relative to healthy cells, such as aplastic anemia.
  • cancer is perhaps the most well known.
  • cardiac ablasion therapy e.g., treating cardiac arrhythmias and atrial fibrillation which are believed to be a significant cause of cerebral stroke.
  • U.S. Pat. No. 6,811,562 describes administering a photoactivatable agent and subjecting cardiac tissue containing the administered agent to laser irradiation having a wavelength from 350 to 700 nm using invasive techniques, e.g., a fiber optic element.
  • PDT photoangioplasty for arterial diseases including de novo atherosclerosis and restinosis (Rockson A G, et al., Circulation, 102:591-596 (2000); Hsiang Y N., et al., J. Endovasc. Surg., 2:365-371 (1995)).
  • endovascular light (730 nm) is delivered through a cylindrical fiber after intravenous administration of motexafin lutetium.
  • PDT is also used for preventing and treatment of intimal hyperlpasia in blood vessels in vivo (see, e.g., U.S. Pat. No. 6,609,014).
  • Age-related macular degeneration is a cause of new blindness. Choroidal neovascularization leads to hemorrhage and fibrosis in a number of ocular diseases.
  • Conventional treatments utilize the argon laser to occlude the leaking vessel by thermal coagulation. However, the percentage of patients eligible for this treatment is limited.
  • PDT is used for treating AMD and involves injecting verteporfin followed by the application of non-thermal light at 692 nm.
  • the choice of therapy usually depends on the location and severity of the disorder, the stage of the disease, as well as the patient's response to the treatment.
  • ECP extracorporeal photopheresis
  • Extracorporeal photopheresis is a leukapheresis-based immunomodulatory therapy that has been approved by the US Food and Drug Administration for the treatment of cutaneous T-cell lymphoma (CTCL).
  • ECP also known as extracorporeal photochemotherapy, is performed at more than 150 centers worldwide for multiple indications. Long-term follow-up data are available from many investigators that indicate ECP produces disease remission and improved survival for CTCL patients.
  • CTCL chronic graft versus host disease
  • ECP use for the treatment of autoimmune disease such as systemic sclerosis and rheumatoid arthritis, is also being explored.
  • ECP is generally performed using the UVAR XTS Photopheresis System developed by Therakos, Inc (Exton, Pa). The process is performed through one intravenous access port and has 3 basic stages: (1) leukapheresis, (2) photoactivation, and (3) reinfusion, and takes 3-4 hours to complete. A typical treatment session would resemble the following sequence of events:
  • the collected WBCs (including approximately 5% of the peripheral blood mononuclear cells) are mixed with heparin, saline, and 8-methoxypsoralen (8-MOP), which intercalates into the DNA of the lymphocytes upon exposure to UVA light and makes them more susceptible to apoptosis when exposed to UVA radiation;
  • 8-MOP 8-methoxypsoralen
  • ECP also induces monocytes to differentiate into dendritic cells capable of phagocytosing and processing the apoptotic T-cell antigens.
  • these activated dendritic cells When these activated dendritic cells are reinfused into the systemic circulation, they may cause a systemic cytotoxic CD8+ T-lymphocyte-mediated immune response to the processed apoptotic T-cell antigens.
  • ECP requires patient to be connected to a machine for hours per treatment. It requires establishing peripheral intravenous line or central venous access, which may be difficult to do in certain disease states such as systemic sclerosis or arthritis. There is also a risk of infection at the venous or central line site, or in the central line catheter. Further, it requires removing typically several hundred milliliters of whole blood from the patient, hence, the treatment is limited to patients who has sufficiently large initial volume of blood to be withdrawn.
  • the American Association of Blood Blanks recommend a limit of extracorporeal volume to 15% of the patient's whole body blood volume. Therefore, the size of the volume that can be treated generally has to be at least 40 kg or more. Risk of contracting blood-born pathogen (Hepatitis, HIV, etc.) due to exposure to contaminated operating system is also a concern.
  • a patient can be treated in vivo with a photosensitive agent followed by the withdrawal of a sample from the patient, treatment with UV radiation in vitro (ex vivo), and reinjecting the patient with the treated sample.
  • This method is known for producing an autovaccine.
  • a method of treating a patient with a photosensitive agent, exposing the patient to an energy source and generating an autovaccine effect wherein all steps are conducted in vivo has not been described. See WO 03/049801, U.S. Pat. No. 6,569,467; U.S. Pat. No. 6,204,058; U.S. Pat. No. 5,980,954; U.S. 6,669,965; U.S. Pat. No. 4,838,852; U.S.
  • a survey of known treatment methods reveals that these methods tend to face a primary difficulty of differentiating between normal cells and target cells when delivering treatment, often due to the production of singlet oxygen which is known to be non-selective in its attack of cells, as well as the need to perform the processes ex vivo, or through highly invasive procedures, such as surgical procedures in order to reach tissues more than a few centimeters deep within the subject.
  • U.S. Pat. No. 5,829,448 describes sequential and simultaneous two photon excitation of photo-agents using irradiation with low energy photons such as infrared or near infrared light (NRI).
  • a single photon and simultaneous two photon excitation is compared for psoralen derivatives, wherein cells are treated with the photo agent and are irradiated with NRI or UV radiation.
  • NRI or UV radiation is known to penetrate tissue to only a depth of a few centimeters.
  • any treatment deep within the subject would necessarily require the use of ex vivo methods or highly invasive techniques to allow the irradiation source to reach the tissue of interest.
  • this patent does not describe initiation energy sources emitting energy other than UV, visible, and near infrared energy; energy upgrading other than within the range corresponding to UV and IR light, and downgrading from high to low energy.
  • U.S. Pat. No. 6,908,591 discloses methods for sterilizing tissue with irradiation to reduce the level of one or more active biological contaminants or pathogens, such as viruses, bacteria, yeasts, molds, fungi, spores, prions or similar agents responsible, alone or in combination, for transmissible spongiform encephalopathies and/or single or multicellular parasites, such that the tissue may subsequently be used in transplantation to replace diseased and/or otherwise defective tissue in an animal.
  • the method may include the use of a sensitizer such as psoralen, a psoralen-derivative or other photosensitizer in order to improve the effectiveness of the irradiation or to reduce the exposure necessary to sterilize the tissue.
  • the method is not suitable for treating a patient and does not teach any mechanisms for stimulating the photosensitizers, indirectly.
  • U.S. Pat. No. 5,957,960 discloses a two-photon excitation device for administering a photodynamic therapy to a treatment site within a patient's body using light having an infrared or near infrared waveband.
  • the reference fails to disclose any mechanism of photoactivation using energy modulation agent that converts the initiation energy to an energy that activates the activatable pharmaceutical agent and also use of other energy wavebands, e.g., X-rays, gamma-rays, electron beam, microwaves or radio waves.
  • U.S. Pat. No. 6,235,508 discloses antiviral applications for psoralens and other photoactivatable molecules. It teaches a method f)r inactivating viral and bacterial contaminants from a biological solution. The method includes mixing blood with a photosensitizer and a blocking agent and irradiating the mixture to stimulate the photosensitizer, inactivating substantially all of the contaminants in the blood, without destroying the red blood cells.
  • the blocking agent prevents or reduces deleterious side reactions of the photosensitizer, which would occur if not in the presence of the blocking agent.
  • the mode of action of the blocking agent is not predominantly in the quenching of any reactive oxygen species, according to the reference.
  • U.S. Pat. No. 6,235,508 suggests that halogenated photosensitizers and blocking agents might be suitable for replacing 8-methoxypsoralen (8-MOP) in photopheresis and in treatment of certain proliferative cancers, especially solid localized tumors accessible via a fiber optic light device or superficial skin cancers.
  • 8-MOP 8-methoxypsoralen
  • the reference fails to address any specific molecules for use in treating lymphomas or any other cancer. Instead, the reference suggests a process of photopheresis for antiviral treatments of raw blood and plasma.
  • U.S. Pat. No. 6,235,508 teaches away from 8-MOP and 4′-aminomethyl-4,5′,8-trimethylpsoralen (AMT) and many other photoactivatable molecules, which are taught to have certain disadvantages.
  • Fluorescing photosensitizers are said to be preferred, but the reference does not teach how to select a system of fluorescent stimulation or photoactivation using fluorescent photosensitizers. Instead, the fluorescing photosensitizer is limited to the intercalator that is binding to the DNA. The reference suggests that fluorescence indicates that such an intercalator is less likely to stimulate oxygen radicals.
  • U.S. published application 2002/0127224 discloses a method for a photodynamic therapy comprising administering light-emitting nanoparticles and a photoactivatable agent, which may be activated by the light re-emitted from the nanoparticles via a two-photon activation event.
  • An initiation energy source is usually a light emitting diode, laser, incandescent lamp, or halogen light, which emits light having a wavelength ranging from 350 to 1100 nm.
  • the initiation energy is absorbed by the nanoparticles.
  • the nanoparticles in turn, re-emit light having a wavelength from 500 to 1100 nm, preferably, UV-A light, wherein the re-emitted energy activates the photoactivatable agent.
  • Kim et al. discloses indirect excitation of a photosensitizing unit (energy acceptor) through fluorescence resonance energy transfer (FRET) from the two-photon absorbing dye unit (energy donor) within an energy range corresponding to 300-850 nm.
  • FRET fluorescence resonance energy transfer
  • initiation energy sources emitting energy other than UV, visible, and near infrared energy; energy upgrading other than within the range corresponding to wavelength of 350-1100 nm, and downgrading from high to low energy.
  • U.S. Pat. No. 6,235,508 further teaches that psoralens are naturally occurring compounds which have been used therapeutically for millennia in Asia and Africa.
  • the action of psoralens and light has been used to treat vitiligo and psoriasis (PUVA therapy; Psoralen Ultra Violet A).
  • Psoralen is capable of binding to nucleic acid double helices by intercalation between base pairs; adenine, guanine, cytosine and thymine (DNA) or uracil (RNA).
  • psoralen in its excited state reacts with a thymine or uracil double bond and covalently attaches to both strands of a nucleic acid helix.
  • the crosslinking reaction appears to be specific for a thymine (DNA) or a uracil (RNA) base. Binding proceeds only if psoralen is intercalated in a site containing thymine or uracil, but an initial photoadduct must absorb a second UVA photon to react with a second thymine or uracil on the opposing strand of the double helix in order to crosslink each of the two strands of the double helix, as shown below. This is a sequential absorption of two single photons as shown, as opposed to simultaneous absorption of two or more photons.
  • 8-MOP is unsuitable for use as an antiviral, because it damages both cells and viruses.
  • Lethal damage to a cell or virus occurs when the psoralen is intercalated into a nucleic acid duplex in sites containing two thymines (or uracils) on opposing strands but only when it sequentially absorbs 2 UVA photons and thymines (or uracils) are present.
  • U.S. Pat. No. 4,748,120 of Wiesehan is an example of the use of certain substituted psoralens by a photochemical decontamination process for the treatment of blood or blood products.
  • Additives such as antioxidants are sometimes used with psoralens, such as 8-MOP, AMT and I-IMT, to scavenge singlet oxygen and other highly reactive oxygen species formed during photoactivation of the psoralens. It is well known that UV activation creates such reactive oxygen species, which are capable of seriously damaging otherwise healthy cells. Much of the viral deactivation may be the result of these reactive oxygen species rather than any effect of photoactivation of psoralens. Regardless, it is believed that no auto vaccine effect has been observed.
  • the best known photoactivatable compounds are derivatives of psoralen or coumarin, which are nucleic acid intercalators.
  • psoralen and coumarin photosensitizers can give rise to alternative chemical pathways for dissipation of the excited state that are either not beneficial to the goal of viral inactivation, or that are actually detrimental to the process.
  • this chemical pathway is likely to lead to the formation of a variety of ring-opened species, such as shown below for coumarin:
  • U.S. Pat. No. 5,216,176 of Heindel discloses a large number of psoralens and coumarins that have some effectiveness as photoactivated inhibitors of epidermal growth factor. Halogens and amines are included among the vast functionalities that could be included in the psoralen/coumarin backbone. This reference is incorporated herein by reference.
  • U. S. Pat. No. 5,984,887 discloses using extracorporeal photopheresis with 8-MOP to treat blood infected with CMV.
  • the treated cells as well as killed and/or attenuated virus, peptides, native subunits of the virus itself which are released upon cell break-up and/or shed into the blood) and/or pathogenic noninfectious viruses are then used to generate an immune response against the virus, which was not present prior to the treatment.
  • one object of the present invention is to provide a method for the treatment of a condition, disorder or disease in a subject that permits treatment of a subject in any area of the body while being non-invasive and having high selectivity for targeted cells relative to healthy cells.
  • a further object of the present invention is to provide a method for treatment of a condition, disorder or disease in a subject which can use any suitable energy source as the initiation energy source to induce a predetermined change in a target structure in a subject in situ to treat said condition, disorder or disease.
  • a further object of the present invention is to provide a method for treatment of a condition, disorder or disease using a modulation agent which adsorbs, intensifies or modifies the initiation energy into an energy that effects a predetermined change in a target structure.
  • initiation energy from at least one source to a target structure in a subject in need of treatment, wherein the initiation energy contacts the target structure and induces a predetermined change in said target structure in situ
  • Yet a further object of the invention is further administer at least one energy modulation agent to said subject which adsorbs, intensifies or modifies said initiation energy into an energy that effects a predetermined change in said target structure.
  • a further object of the present invention is to provide a method for treatment of a condition, disorder or disease which can use any suitable energy source as the initiation energy source to activate the activatable pharmaceutical agent and thereby cause a predetermined change in a target structure to treat a condition, disorder or disease.
  • a further object of the present invention is to provide a method for treatment of a condition, disorder or disease using an energy cascade to activate an activatable pharmaceutical agent that then treats cells suffering from a condition, disorder or disease.
  • a further object of the present invention is to provide a method for generating an autovaccine effect in a subject, which can be in vivo thus avoiding the need for ex vivo treatment of subject tissues or cells, or can be ex vivo.
  • a further object of the present invention is to provide a method for generating an autovaccine effect in a subject, which can be in vivo thus avoiding the need for ex vivo treatment of subject tissues or cells, or can be ex vivo.
  • a further object of the present invention is to provide a computer implemented system for performing the methods of the present invention.
  • a still further object of the present invention is to provide a kit and a pharmaceutical composition for use in the present invention methods.
  • initiation energy from at least one source to a target structure in a subject in need of treatment, wherein the initiation energy contacts the target structure and induces a predetermined change in said target structure in situ
  • said predetermined change modifies the target structure and modulates the biological activity of the target structure.
  • a further object of the present invention is to provide a method for modifying a target structure which mediates or is associated with a biological activity, comprising:
  • a further object of the present invention is to provide such methods which can use any suitable energy source as the initiation energy source in combination with plasmonics materials to activate the activatable pharmaceutical agent and thereby cause the predetermined change.
  • a further object of the present invention is to provide such methods using plasmonics in an energy cascade to activate an activatable pharmaceutical agent that then cause the predetermined change.
  • a further object of the present invention is to provide such methods for in situ generation of energy which causes, either directly or indirectly, the predetermined change.
  • a further object of the present invention is to provide a method for the treatment of a cell proliferation disorder that permits treatment of a subject in any area of the body while being non-invasive and having high selectivity for targeted cells relative to healthy cells through the use of exciton-plasmon enhancement.
  • a further object of the present invention is to provide a method for treatment of a condition, disorder or disease which can use any suitable energy source as the initiation energy source in combination with exciton-plasmon enhancement to activate the activatable pharmaceutical agent and thereby cause a predetermined change to treat cells suffering from a condition, disorder or disease.
  • a further object of the present invention is to provide a method for treatment of a condition, disorder or disease using exciton-plasmon enhancement in an energy cascade to activate an activatable pharmaceutical agent that then treats cells suffering from a condition, disorder or disease.
  • Another object of the invention is a method for treating a condition, disorder, or disease associated with a target structure in a subject, comprising:
  • the treated condition, disorder, or disease may or may not be significantly mediated by abnormal cellular proliferation and said predetermined change does not have to substantially affect cellular proliferation.
  • Yet another object of the invention is a method for modifying a target structure which mediates or is associated with a biological activity, comprising:
  • the plasmonics-active agent enhances or modifies the applied initiation energy, such that the enhanced initiation energy activates the activatable agent
  • FIG. 1 provides an exemplary electromagnetic spectrum in meters (1 nm equals 10 ⁇ 9 meters).
  • FIG. 2A and FIG. 2B are graphical representations of the depth of penetration of various wavelengths of energy into living tissue
  • FIG. 3 illustrates a system according to one exemplary embodiment of the present invention.
  • FIG. 4 illustrates an exemplary computer implemented system according to an embodiment of the present invention.
  • FIG. 5 illustrates an exemplary computer system ( 1201 ) for implementing various embodiments of the present invention.
  • FIG. 6 is a graphical representation of plasmonic nanostructures and their theoretical electromagnetic enhancement at different excitation wavelengths.
  • FIG. 7 provides representative embodiments of plasmonics photo-active probes useful in the present invention.
  • FIG. 8 is a graphical explanation of the plasmonics-enhanced effect of photospectral therapy used in the present invention.
  • FIG. 9 provides representative embodiments of plasmonics-active nanostructures.
  • FIG. 10 is a graphical representation of one embodiment of a PEPST probe with remote drug release.
  • FIG. 11 is a graphical representation of several embodiments of PEPST probes with various linkers for remote drug release.
  • FIG. 12 is a graphical representation of several embodiments of plasmonics photo-active probes with bioreceptors.
  • FIG. 13 is a graphical representation of the “therapeutic window” in tissue and absorption spectra of biological components.
  • FIG. 14 is a graphical representation of an embodiment of the energy modulation agent(or excitation energy converter/EEC)-photo activator (PA) system of the present invention.
  • FIG. 15 is a graphical representation of several embodiments of plasmonics photo-active energy modulation agent-PA probes.
  • FIG. 16 shows structures of various preferred embodiments of gold complexes exhibiting XEOL.
  • FIG. 17 shows the structure of a further embodiment of compound exhibiting XEOL, namely a tris-8-hydroxyquinoline-aluminum complex.
  • FIG. 17 is a graphical representation of a plasmonics-enhanced mechanism for a photo-active energy modulation agent-PA probe of the present invention.
  • FIG. 19 is a graph showing excitation and emission fluorescence spectra of psoralens.
  • FIG. 20 is a graphical representation of an embodiment of a PEPST energy modulation agent-PA system with detachable bond.
  • FIG. 21 is a graphical representation of an embodiment of PEPST probes for dual plasmonic excitation.
  • FIG. 22 is a graphical representation of an embodiment of a use of encapsulated photoactive agents.
  • FIG. 23 is a simplified graphical representation of the use of the present invention principle of non-invasive PEPST modality.
  • FIG. 24 is an photomicrograph showing nanocaps (half-nanoshells) comprising polystyrene nanospheres coated with silver.
  • FIG. 25 shows various schematic embodiments of basic EIP probes.
  • FIG. 26 is a graphical representation of fluorescence spectra of PAH compounds.
  • FIG. 27 is a graph showing the XEOL of Eu doped in BaFBr matrix.
  • FIG. 28 provides further embodiments of schematic designs of EIP probes.
  • FIG. 29 is a graphical representation of various embodiments of basic EPEP probes.
  • FIG. 30 is a graphical representation of various embodiments of basic EPEP probes.
  • FIG. 31 is a graphical representation of various embodiments of EPEP probes having NPs, NWs and NRs.
  • FIG. 32 is a graphical representation of various embodiments of EPEP probes having NPs, NWs, NRs and bioreceptors.
  • FIG. 33 is a graphical representation of an embodiment of EPEP probes having NPs and multiple NWs.
  • FIG. 34 shows photo-active probes in which a photo-active molecule is bound to plasmonics probes.
  • FIG. 35 shows plasmonics photo-active probes that have a dielectric layer between the metal and the UC materials.
  • the present invention sets forth a novel method of modifying a target structure which mediates or is associated with a biological activity, which includes treating a condition, disorder or disease in a subject, that is effective, specific, and has few side-effects.
  • Those cells suffering from a condition, disorder or disease are referred to herein as the target cells.
  • the present invention provides method for modifying a target structure which mediates or is associated with a biological activity comprising:
  • initiation energy from at least one source to a target structure in a subject in need of treatment, wherein the initiation energy contacts the target structure and induces a predetermined change in said target structure in situ
  • said predetermined change modifies the target structure and modulates the biological activity of the target structure.
  • a further object of the present invention is to provide a method for modifying a target structure which mediates or is associated with a biological activity, comprising:
  • the present invention provides methods for the treatment of a condition, disorder or disease, in which an initiation energy source provides an initiation energy that causes the predetermined cellular changes directly to treat target cells within a subject.
  • the initiation energy source is applied indirectly via an energy modulation agent, preferably in proximity to the target cells.
  • the present invention further provides methods for the treatment of a condition, disorder or disease, in which an initiation energy source provides an initiation energy that activates an activatable pharmaceutical agent to treat target cells within the subject.
  • the initiation energy source is applied indirectly via an energy modulation agent to the activatable pharmaceutical agent, preferably in proximity to the target cells.
  • the present invention also provides methods for the treatment of a condition, disorder or disease in which an initiation energy source is enhanced or modified by a plasmonic-active agent, such that the enhanced initiation energy activates the pharmaceutical agent in situ.
  • an object of the present invention is to modify a target structure which mediates or is associated with a biological activity, and in a preferred embodiment to treat a condition, disorder or disease, in a subject using photobiomodulation.
  • exemplary conditions, disorders or diseases may include, but are not limited to, cancer, autoimmune diseases, soft and bone tissue injury, chronic pain, wound healing, nerve regeneration, viral and bacterial infections, fat deposits (liposuction), varicose veins, enlarged prostate, retinal injuries and other ocular diseases, Parkinson's disease, and behavioral, perceptional and cognitive disorders.
  • Exemplary conditions also may include nerve (brain) imaging and stimulation, a direct control of brain cell activity with light, control of cell death (apoptosis), and alteration of cell growth and division.
  • a method in accordance with the present invention utilizes an initiation energy from at least one source applied to a target structure in a subject in need of treatment, wherein the initiation energy contacts the target structure and induces a predetermined change in said target structure in situ, thus modifying a target structure which mediates or is associated with a biological activity, preferably treating a condition, disorder or disease.
  • the initiation energy can preferably penetrate completely through the subject and can be applied from a single source or more than one source.
  • Exemplary initiation energy may be UV radiation, visible light, infrared radiation (IR), x-rays, gamma rays, an electron beam, microwaves or radio waves.
  • a plasmonics-active agent enhances or modifies the applied initiation energy, such that the enhanced initiation energy causes the predetermined change in said target structure.
  • a plasmonics-active agent enhances or modifies the applied initiation energy, such that the enhanced initiation energy is absorbed, intensified or modified by the modulation agent into the energy that effects the predetermined change in said target structure.
  • a method in accordance with the present invention utilizes the principle of energy transfer to and among molecular agents to control delivery and activation of cellular changes by irradiation such that delivery of the desired effect is more intensified, precise, and effective than the conventional techniques.
  • At least one energy modulation agent can be administered to the subject which adsorbs, intensifies or modifies said initiation energy into an energy that effects a predetermined cellular change in said target structure.
  • the energy modulation agent may be located around, on, or in said target structure.
  • the energy modulation agent can transform a photonic initiation energy into a photonic energy that effects a predetermined change in said target structure.
  • the energy modulation agent decreases the wavelength of the photonic initiation energy.
  • the energy modulation agent can increase the wavelength of the photonic initiation energy.
  • the modulation agent is one or more members selected from a biocompatible fluorescing metal nanoparticle, fluorescing metal oxide nanoparticle, fluorescing dye molecule, gold nanoparticle, silver nanoparticle, gold-coated silver nanoparticle, a water soluble quantum dot encapsulated by polyamidoamine dendrimers, a luciferase, a biocompatible phosphorescent molecule, a combined electromagnetic energy harvester molecule, and a lanthanide chelate exhibiting intense luminescence.
  • Another object of the present invention is to treat a condition, disorder or disease in a subject using an activatable pharmaceutical agent.
  • exemplary conditions, disorders or diseases may include, but are not limited to, cancer, autoimmune diseases, cardiac ablasion (e.g., cardiac arrhythmia and atrial fibrillation), photoangioplastic conditions (e.g., de novo atherosclerosis, restinosis), intimal hyperplasia, arteriovenous fistula, macular degeneration, psoriasis, acne, hopecia areata, portwine spots, hair removal, rheumatoid and inflammatory arthrisis, joint conditions, lymph node conditions, and cognitive and behavioral conditions.
  • cardiac ablasion e.g., cardiac arrhythmia and atrial fibrillation
  • photoangioplastic conditions e.g., de novo atherosclerosis, restinosis
  • intimal hyperplasia arteriovenous fistula, macular degeneration
  • the present invention provides methods utilizing the principle of energy transfer to and among molecular agents to control delivery and activation of pharmaceutically active agents such that delivery of the desired pharmacological effect is more focused, precise, and effective than the conventional techniques.
  • the initiation energy source is applied directly or indirectly (via a modulation agent) to the activatable pharmaceutical agent, preferably in proximity to the target cells.
  • the phrase “applied indirectly” when referring to the application of the initiation energy, means the penetration by the initiation energy into the subject beneath the surface of the subject and to the modulation agent and/or activatable pharmaceutical agent within a subject.
  • the initiation energy interacts with a previously administered energy modulation agent which then activates the predetermined cellular changes.
  • the initiation energy interacts with a previously administered energy modulation agent which then activates the activatable pharmaceutical agent.
  • the initiation energy itself activates the activatable pharmaceutical agent.
  • the initiation energy source cannot be within line-of-sight of the modulation agent and/or the activatable pharmaceutical agent.
  • cannot be within line-of-sight is meant that if a hypothetical observer were located at the location of the modulation agent or the activatable pharmaceutical agent, that observer would be unable to see the source of the initiation energy.
  • subject is not intended to be limited to humans, but may also include animals, plants, or any suitable biological organism.
  • a disease or condition refers to a condition, disorder or disease that may include, but are not limited to, cancer, soft and bone tissue injury, chronic pain, wound healing, nerve regeneration, viral and bacterial infections, fat deposits (liposuction), varicose veins, enlarged prostate, retinal injuries and other ocular diseases, Parkinson's disease, and behavioral, perceptional and cognitive disorders.
  • exemplary conditions also may include nerve (brain) imaging and stimulation, a direct control of brain cell activity with light, control of cell death (apoptosis), and alteration of cell growth and division.
  • exemplary a condition, disorder or disease may include, but are not limited to, cardiac ablasion (e.g., cardiac arrhythmia and atrial fibrillation), photoangioplastic conditions (e.g., de novo atherosclerosis, restinosis), intimal hyperplasia, arteriovenous fistula, macular degeneration, psoriasis, acne, hopecia areata, portwine spots, hair removal, rheumatoid and inflammatory arthritis, joint conditions, and lymph node conditions.
  • cardiac ablasion e.g., cardiac arrhythmia and atrial fibrillation
  • photoangioplastic conditions e.g., de novo atherosclerosis, restinosis
  • intimal hyperplasia e.g., arteriovenous fistula, macular degeneration, psoriasis, acne, hopecia areata, portwine spots, hair removal, rheumatoid and inflammatory arthritis, joint conditions, and lymph no
  • target structure refers to an eukaryotic cell, prokaryotic cell, a subcellular structure, such as a cell membrane, a nuclear membrane, cell nucleus, nucleic acid, mitochondria, ribosome, or other cellular organelle or component, an extracellular structure, virus or prion, and combinations thereof.
  • predetermined cellular change will depend on the desired pharmaceutical outcome.
  • exemplary cellular changes may include, but are not limited to, apoptosis, necrosis, up-regulation of certain genes, down-regulation of certain genes, secretion of cytokines, alteration of cytokine receptor responses, regulation of cytochrome c oxidase and flavoproteins, activation of mitochondria, stimulation antioxidant protective pathway, modulation of cell growth and division, alteration of firing pattern of nerves, alteration of redox properties, generation of reactive oxygen species, modulation of the activity, quantity, or number of intracellular components in a cell, modulation of the activity, quantity, or number of extracellular components produced by, excreted by, or associated with a cell, or a combination thereof.
  • Predetermined cellular changes may or may not result in destruction or inactivation of the target structure.
  • an “energy modulation agent” refers to an agent that is capable of receiving an energy input from a source and then re-emitting a different energy to a receiving target. Energy transfer among molecules may occur in a number of ways. The form of energy may be electronic, thermal, electromagnetic, kinetic, or chemical in nature. Energy may be transferred from one molecule to another (intermolecular transfer) or from one part of a molecule to another part of the same molecule (intramolecular transfer). For example, a modulation agent may receive electromagnetic energy and re-emit the energy in the form of thermal energy. In preferred embodiments, the energy modulation agent receives higher energy (e.g. x-ray) and re-emits in lower energy (e.g. UV-A).
  • higher energy e.g. x-ray
  • UV-A lower energy
  • Some modulation agents may have a very short energy retention time (on the order of fs, e.g. fluorescent molecules) whereas others may have a very long half-life (on the order of minutes to hours, e.g. luminescent or phosphorescent molecules).
  • Suitable energy modulation agents include, but are not limited to, a biocompatible fluorescing metal nanoparticle, fluorescing dye molecule, gold nanoparticle, a water soluble quantum dot encapsulated by polyamidoamine dendrimers, a luciferase, a biocompatible phosphorescent molecule, a combined electromagnetic energy harvester molecule, and a lanthanide chelate capable of intense luminescence.
  • a biocompatible fluorescing metal nanoparticle fluorescing dye molecule
  • gold nanoparticle gold nanoparticle
  • a water soluble quantum dot encapsulated by polyamidoamine dendrimers a luciferase
  • a biocompatible phosphorescent molecule
  • the modulation agents may further be coupled to a carrier for cellular targeting purposes.
  • a biocompatible molecule such as a fluorescing metal nanoparticle or fluorescing dye molecule that emits in the UV-A band, may be selected as the energy modulation agent.
  • the energy modulation agent may be preferably directed to the desired site (e.g. a tumor) by systemic administration to a subject.
  • a UV-A emitting energy modulation agent may be concentrated in the tumor site by physical insertion or by conjugating the UV-A emitting energy modulation agent with a tumor specific carrier, such as a lipid, chitin or chitin-derivative, a chelate or other functionalized carrier that is capable of concentrating the UV-A emitting source in a specific target tumor.
  • the energy modulation agent can be used alone or as a series of two or more energy modulation agents wherein the energy modulation agents provide an energy cascade.
  • the first energy modulation agent in the cascade will absorb the activation energy, convert it to a different energy which is then absorbed by the second energy modulation in the cascade, and so forth until the end of the cascade is reached with the final energy modulation agent in the cascade emitting the energy necessary to activate the activatable pharmaceutical agent.
  • Exemplary energy modulation agents may include, but are not limited to, at least one energy modulation agent selected from the group consisting of a biocompatible fluorescing metal nanoparticle, fluorescing metal oxide nanoparticle, fluorescing dye molecule, gold nanoparticle, silver nanoparticle, gold-coated silver nanoparticle, a water soluble quantum dot encapsulated by polyamidoamine dendrimers, a luciferase, a biocompatible phosphorescent molecule, a combined electromagnetic energy harvester molecule, and a lanthanide chelate exhibiting intense luminescence.
  • at least one energy modulation agent selected from the group consisting of a biocompatible fluorescing metal nanoparticle, fluorescing metal oxide nanoparticle, fluorescing dye molecule, gold nanoparticle, silver nanoparticle, gold-coated silver nanoparticle, a water soluble quantum dot encapsulated by polyamidoamine dendrimers, a luciferase, a biocompatible phosphor
  • an “activatable pharmaceutical agent” is an agent that normally exists in an inactive state in the absence of an activation signal. When the agent is activated by a matching activation signal under activating conditions, it is capable of effecting the desired pharmacological effect on a target cell (i.e. preferably a predetermined cellular change).
  • Signals that may be used to activate a corresponding agent may include, but are not limited to, photons of specific wavelengths (e.g. x-rays, or visible light), electromagnetic energy (e.g. radio or microwave), thermal energy, acoustic energy, or any combination thereof.
  • Activation of the agent may be as simple as delivering the signal to the agent or may further premise on a set of activation conditions.
  • an activatable pharmaceutical agent such as a photosensitizer, may be activated by UV-A radiation. Once activated, the agent in its active-state may then directly proceed to effect a cellular change.
  • activation may further premise upon other conditions, mere delivery of the activation signal may not be sufficient to bring about the desired cellular change.
  • a photoactive compound that achieves its pharmaceutical effect by binding to certain cellular structure in its active state may require physical proximity to the target cellular structure when the activation signal is delivered.
  • delivery of the activation signal under non-activating conditions will not result in the desired pharmacologic effect.
  • Some examples of activating conditions may include, but are not limited to, temperature, pH, location, state of the cell, presence or absence of co-factors.
  • activatable pharmaceutical agent greatly depends on a number of factors such as the desired cellular change, the desired form of activation, as well as the physical and biochemical constraints that may apply.
  • exemplary activatable pharmaceutical agents may include, but are not limited to, agents that may be activated by photonic energy, electromagnetic energy, acoustic energy, chemical or enzymatic reactions, thermal energy, or any other suitable activation mechanisms.
  • the activatable pharmaceutical agent When activated, may effect cellular changes that include, but are not limited to, apoptosis, redirection of metabolic pathways, up-regulation of certain genes, down-regulation of certain genes, secretion of cytokines, alteration of cytokine receptor responses, or combinations thereof.
  • an activatable pharmaceutical agent may achieve its desired effect. Such mechanisms may include direct action on a predetermined target as well as indirect actions via alterations to the biochemical pathways.
  • a preferred direct action mechanism is by binding the agent to a critical cellular structure such as nuclear DNA, mRNA, rRNA, ribosome, mitochondrial DNA, or any other functionally important structures.
  • Indirect mechanisms may include releasing metabolites upon activation to interfere with normal metabolic pathways, releasing chemical signals (e.g. agonists or antagonists) upon activation to alter the targeted cellular response, and other suitable biochemical or metabolic alterations.
  • the treatment of the present invention can be by the unique methods described in U.S. application Ser. No. 11/935,655, filed Nov. 6, 2007 (incorporated by reference above), or by a modified version of a conventional treatment such as PDT, but using a plasmonics-active agent to enhance the treatment by modifying or enhancing the applied energy or, in the case of using an energy modulation agent, modifying either the applied energy, the emitted energy from the energy modulation agent, or both.
  • the activatable pharmaceutical agent is capable of chemically binding to the DNA or mitochondria at a therapeutically effective amount.
  • the activatable pharmaceutical agent preferably a photoactivatable agent, is exposed in situ to an activating energy emitted from an energy modulation agent, which, in turn receives energy from an initiation energy source.
  • Suitable activatable agents include, but are not limited to, photoactive agents, sono-active agents, thermo-active agents, and radio/microwave-active agents.
  • An activatable agent may be a small molecule; a biological molecule such as a protein, a nucleic acid or lipid; a supramolecular assembly; a nanoparticle; or any other molecular entity having a pharmaceutical activity once activated.
  • the activatable agent may be derived from a natural or synthetic origin. Any such molecular entity that may be activated by a suitable activation signal source to effect a predetermined cellular change may be advantageously employed in the present invention.
  • Suitable photoactive agents include, but are not limited to: psoralens and psoralen derivatives, pyrene cholesteryloleate, acridine, porphyrin, fluorescein, rhodamine, 16-diazorcortisone, ethidium, transition metal complexes of bleomycin, transition metal complexes of deglycobleomycin, organoplatinum complexes, alloxazines such as 7,8-dimethyl-10-ribityl isoalloxazine (riboflavin), 7,8,10-trimethylisoalloxazine (lumiflavin), 7,8-dimethylalloxazine (lumichrome), isoalloxazine-adenine dinucleotide (flavine adenine dinucleotide [FAD]), alloxazine mononucleotide (also known as flavine mononucleotide [FMN] and riboflavine-5-
  • Endogenously-based derivatives include synthetically derived analogs and homologs of endogenous photoactivated molecules, which may have or lack lower (1 to 5 carbons) alkyl or halogen substituents of the photosensitizers from which they are derived, and which preserve the function and substantial non-toxicity. Endogenous molecules are inherently non-toxic and may not yield toxic photoproducts after photoradiation.
  • Table 1 lists some photoactivatable molecules capable of being photoactivated to induce an auto vaccine effect.
  • Table 2 lists some additional endogenous photoactivatable molecules.
  • FIG. 1 provides an exemplary electromagnetic spectrum in meters (1 nm equals meters).
  • the activatable pharmaceutical agent and the energy modulation agent can be distinct and separate, it will be understood that the two agents need not be independent and separate entities. In fact, the two agents may be associated with each other via a number of different configurations. Where the two agents are independent and separately movable from each other, they generally interact with each other via diffusion and chance encounters within a common surrounding medium. Where the activatable pharmaceutical agent and the energy modulation agent are not separate, they may be combined into one single entity.
  • the initiation energy source can be any energy source capable of providing energy at a level sufficient to cause cellular changes directly or via a modulation agent which transfer the initiation energy to energy capable of causing the predetermined cellular changes.
  • the initiation energy source can be any energy source capable of providing energy at a level sufficient activate the activatable agent directly, or to provide the energy to a modulation agent with the input needed to emit the activation energy for the activatable agent (indirect activation).
  • Preferable initiation energy sources include, but are not limited to, UV-A lamps or fiber optic lines, a light needle, an endoscope, and a linear accelerator that generates x-ray, gamma-ray, or electron beams. In a preferred embodiment the initiation energy capable of penetrating completely through the subject.
  • the phrase “capable of penetrating completely through the subject” is used to refer to energy that can penetrate to any depth within the subject to activate the activatable pharmaceutical agent. It is not required that the any of the energy applied actually pass completely through the subject, merely that it be capable of doing so in order to permit penetration to any desired depth to activate the activatable pharmaceutical agent.
  • Exemplary initiation energy sources that are capable of penetrating completely through the subject include, but are not limited to, UV light, visible light, IR radiation, x-rays, gamma rays, electron beams, microwaves and radio waves.
  • An additional embodiment of the present invention is to provide a method for treatment of a condition, disease or disorder by the in-situ generation of energy in a subject in need thereof, where the energy generated can be used directly to effect a change thereby treating the condition, disease or disorder, or the energy can be used to activate an activatable pharmaceutical agent, which upon activation effects a change thereby treating the condition, disease or disorder.
  • the energy can be generated in-situ by any desired method, including, but not limited to, chemical reaction such as chemiluminescence, or by conversion of an energy applied to the subject externally, which is converted in-situ to a different energy (of lower or higher energy than that applied), through the use of one or more energy modulation agents.
  • a further embodiment of the present invention combines the treatment of a condition, disease or disorder with the generation of heat in the affected target structure in order to enhance the effect of the treatment.
  • a photoactivatable pharmaceutical agent such as a psoralen or derivative thereof
  • this initiation energy can be of any type, so long as it can be converted to an energy suitable for activating the pharmaceutical compound.
  • an energy is applied that causes heating of the target structure. In the case of a cell proliferation disorder such as cancer, the heating would increase the proliferation rate of the cancer cells.
  • the heat can be generated in any desired manner.
  • the heat can be generated using the application of microwaves or NIR energy to the target structure or by the use of use of nanoparticles of metal or having metal shells.
  • magnetic metal nanoparticles can be targeted to cancer cells using conventional techniques, then used to generate heat by application of a magnetic field to the subject under controlled conditions.
  • the source of the initiation energy can be a radiowave emitting nanotube, such as those described by K. Jensen, J. Weldon, H. Garcia, and A. Zettl in the Department of Physics at the University of California at Berkeley (see http://socrates.berkeley.edu/ ⁇ argon/nanoradio/radio.html, the entire contents of which are hereby incorporated by reference).
  • nanotubes can be administered to the subject, and preferably would be coupled to the activatable pharmaceutical agent or the energy modulation agent, or both, or be located in proximity of a target cell such that upon application of the initiation energy, the nanotubes would accept the initiation energy (preferably radiowaves), then emit radiowaves in close proximity to the activatable pharmaceutical agent, or in close proximity to the energy modulation agent, or to the target cell to then cause the predetermined cellular changes or activation of the activatable pharmaceutical agent.
  • the nanotubes would act essentially as a radiowave focusing or amplification device in close proximity to the activatable pharmaceutical agent or energy modulation agent or the target cell.
  • the energy emitting source may be an energy modulation agent that emits energy in a form suitable for absorption by the transfer agent or a target cell.
  • the initiation energy source may be acoustic energy and one energy modulation agent may be capable of receiving acoustic energy and emitting photonic energy (e.g. sonoluminescent molecules) to be received by another energy modulation agent that is capable of receiving photonic energy.
  • photonic energy e.g. sonoluminescent molecules
  • transfer agents that receive energy at x-ray wavelength and emit energy at UV wavelength, preferably at UV-A wavelength.
  • a plurality of such energy modulation agents may be used to form a cascade to transfer energy from initiation energy source via a series of energy modulation agents to activate the activatable agent or the predetermined cellular change.
  • Signal transduction schemes as a drug delivery vehicle may be advantageously developed by careful modeling of the cascade events coupled with metabolic pathway knowledge to sequentially or simultaneously cause the predetermined cellular change or activate multiple activatable pharmaceutical agents to achieve multiple-point alterations in cellular function.
  • Photoactivatable agents may be stimulated by an energy source, such as irradiation, resonance energy transfer, exciton migration, electron injection, or chemical reaction, to an activated energy state that is capable of effecting the predetermined cellular change desired.
  • an energy source such as irradiation, resonance energy transfer, exciton migration, electron injection, or chemical reaction
  • the photoactivatable agent upon activation, binds to DNA or RNA or other structures in a cell.
  • the activated energy state of the agent is capable of causing damage to cells, inducing apoptosis.
  • One preferred method of treating a condition, disorder or disease mediated by a target structure in a subject comprises:
  • Another preferred method for treating a condition, disorder or disease mediated by a target structure in a subject comprises:
  • the concept of multi-photon excitation is based on the idea that two or more photons of low energy can excite a fluorophore in a quantum event, resulting in the emission of a fluorescence photon, typically at a higher energy than the two or more excitatory photons.
  • This concept was first described by Maria Göppert-Mayer in her 1931 doctoral thesis. However, the probability of the near-simultaneous absorption of two or more photons is extremely low. Therefore a high flux of excitation photons is typically required, usually a femtosecond laser. This had limited the range of practical applications for the concept.
  • an infrared laser beam is focused through an objective lens.
  • the Ti-sapphire laser normally used has a pulse width of approximately 100 femtoseconds and a repetition rate of about 80 MHz, allowing the high photon density and flux required for two photons absorption and is tunable across a wide range of wavelengths.
  • Two-photon technology is patented by Winfried Denk, James Strickler and Watt Webb at Cornell University.
  • TPEF two-photon excited fluorescence
  • NLT non-linear transmission
  • the most commonly used fluorophores have excitation spectra in the 400-500 nm range, whereas the laser used to excite the fluorophores lies in the ⁇ 700-1000 nm (infrared) range. If the fluorophore absorbs two infrared photons simultaneously, it will absorb enough energy to be raised into the excited state. The fluorophore will then emit a single photon with a wavelength that depends on the type of fluorophore used (typically in the visible spectrum). Because two photons need to be absorbed to excite a fluorophore, the probability of emission is related to the intensity squared of the excitation beam.
  • the radiative signal may be of the exact energy required to active the photoactive agent.
  • the radiative energy may be directly targeted at the desired coordinate or region where the photoactive agent is present.
  • the initiation energy source in this embodiment may be, for example, x-rays, gamma rays, an electron beam, microwaves or radio waves.
  • the radiative signal may be of a lower energy than the excitation energy of the photoactive agent.
  • the radiative signal does not have sufficient energy to activate the photoactive agent in a conventional way.
  • Activation of the photoactive agent may be achieved via an “energy upgrade” mechanism such as the multi-photon mechanism described above.
  • Activation of the photoactive agent may further be mediated by an intermediary energy transformation agent.
  • the radiative energy may first excite a fluorophore that emits a photon at the right energy that excites the photoactive agent.
  • the signal is delivered to the target photoactive agent by way of this intermediary agent. In this way, in addition to energy upgrading (and downgrading, as described below), a signal relay mechanism is also introduced.
  • the initiation energy source may be x-rays, gamma rays, an electron beam, microwaves or radio waves. Also, in one embodiment, if the initiation energy is an infrared energy, the energy activating the activatable agent is not UV or visible light energy.
  • another preferred method for treating a condition, disease, or disorder mediated by a target structure in a subject comprises:
  • the energy upgrades are obtained via 2, 3, 4, or 5 simultaneouse photon absorptions.
  • Yet another preferred method for treating a condition, diseases, or disorder mediated by a target structure in a subject comprises:
  • the radiative energy may be of a higher energy than the excitation energy of the photoactive agent.
  • the photoactive agent may be activated via an “energy downgrade” mechanism.
  • two lower energy photons having energy x may be absorbed by an agent to excite the agent from ground state E0 to a higher energy state E2.
  • the agent may then relax down to an intermediate energy state E1 by emitting a photon having an energy y that is equal to the energy gap between E2 and E1, where y is less than x.
  • Other mechanisms of energy downgrade may be mediated by energy transformation agents such as quantum dots, nanotubes, or other agents having suitable photo-radiation properties.
  • the initiation energy source may be, for example, UV radiation, visible light, infrared radiation, x-rays, gamma rays, an electron beam, microwaves or radio waves.
  • yet another preferred method for treating a condition, disease, or disorder mediated by a target structure in a subject comprises:
  • the present invention provides a method for treating a condition, disorder or disease mediated by a target structure in a subject, comprising:
  • initiation energy applied and activatable pharmaceutical agent upon activation produce insufficient singlet oxygen in the subject to produce cell lysis, and wherein the initiation energy activates the activatable pharmaceutical agent in situ
  • the present invention provides a method for treating a condition, disorder or disease mediated by a target structure in a subject, comprising:
  • initiation energy applied and activatable pharmaceutical agent upon activation produce insufficient singlet oxygen in the subject to produce cell lysis, and wherein the initiation energy activates the activatable pharmaceutical agent by the multi photon absorption event in situ
  • the amount of singlet oxygen required to cause cell lysis, and thus cell death is 0.32 ⁇ 10 ⁇ 3 mol/liter or more, or 10 9 singlet oxygen molecules/cell or more.
  • the level of singlet oxygen production caused by the initiation energy used or activatable pharmaceutical agent upon activation be less than level needed to cause cell lysis.
  • One advantage is that multiple wavelengths, of emitted radiation may be used to selectively stimulate one or more photoactivatatble agents or energy modulation agents capable of stimulating the one or more photoactivatable agents.
  • the energy modulation agent is preferably stimulated at a wavelength and energy that causes little or no damage to healthy cells, with the energy from one or more energy modulation agents being transferred, such as by Foerster Resonance Energy Transfer, to the photoactivatable agents that damage the cell and cause the onset of the desired cellular change, e.g., apoptosis of the cells.
  • Another advantage is that side effects can be greatly reduced by limiting the production of free radicals, singlet oxygen, hydroxides and other highly reactive groups that are known to damage healthy cells. Furthermore, additional additives, such as antioxidants, may be used to further reduce undesired effects of irradiation.
  • Resonance Energy Transfer is an energy transfer mechanism between two molecules having overlapping emission and absorption bands. Electromagnetic emitters are capable of converting an arriving wavelength to a longer wavelength. For example, UV-B energy absorbed by a first molecule may be transferred by a dipole-dipole interaction to a UV-A-emitting molecule in close proximity to the UV-B-absorbing molecule. Alternatively, a material absorbing a shorter wavelength may be chosen to provide RET to a non-emitting molecule that has an overlapping absorption band with the transferring molecule's emission band. Alternatively, phosphorescence, chemiluminescence, or bioluminescence may be used to transfer energy to a photoactivatable molecule.
  • the administering of the initiation energy source means the administration of an agent, that itself produces the initiation energy, in a manner that permits the agent to arrive at the target cell within the subject without being surgically inserted into the subject.
  • the administration can take any form, including, but not limited to, oral, intravenous, intraperitoneal, inhalation, etc.
  • the initiation energy source in this embodiment can be in any form, including, but not limited to, tablet, powder, liquid solution, liquid suspension, liquid dispersion, gas or vapor, etc.
  • the initiation energy source includes, but is not limited to, chemical energy sources, nanoemitters, nanochips, and other nanomachines that produce and emit energy of a desired frequency.
  • chemical energy sources such as the Molecular Switch (or Mol-Switch) work by Dr. Keith Firman of the EC Research and Development Project, or the work of Cornell et al. (1997) who describe the construction of nanomachines based around ion-channel switches only 1.5 nm in size, which use ion channels formed in an artificial membrane by two gramicidin molecules: one in the lower layer of the membrane attached to a gold electrode and one in the upper layer tethered to biological receptors such as antibodies or nucleotides.
  • the receptor captures a target molecule or cell
  • the ion channel is broken, its conductivity drops, and the biochemical signal is converted into an electrical signal.
  • These nanodevices could also be coupled with the present invention to provide targeting of the target cell, to deliver the initiation energy source directly at the desired site.
  • the present invention includes the administration of a source of chemical energy such as chemiluminescence, phosphorescence or bioluminescence.
  • the source of chemical energy can be a chemical reaction between two or more compounds, or can be induced by activating a chemiluminescent, phosphorescent or bioluminescent compound with an appropriate activation energy, either outside the subject or inside the subject, with the chemiluminescence, phosphorescence or bioluminescence being allowed to activate the activatable pharmaceutical agent in vivo after administration.
  • the activatable pharmaceutical agent and the source of chemical energy can be administered. The administration can be performed sequentially in any order or simultaneously.
  • the administration of the chemical energy source can be performed after activation outside the subject, with the lifetime of the emission of the energy being up to several hours for certain types of phosphorescent materials for example.
  • nanoparticles or nanoclusters of certain atoms may be introduced such that are capable of resonance energy transfer over comparatively large distances, such as greater than one nanometer, more preferably greater than five nanometers, even more preferably at least 10 nanometers.
  • resonance energy transfer may have a large enough “Foerster” distance (R 0 ), such that nanoparticles in one part of a cell are capable of stimulating activation of photoactivatable agents disposed in a distant portion of the cell, so long as the distance does not greatly exceed R 0 .
  • R 0 “Foerster” distance
  • gold nanospheres having a size of 5 atoms of gold have been shown to have an emission band in the ultraviolet range, recently.
  • an aggressive cell proliferation disorder has a much higher rate of mitosis, which leads to selective destruction of a disproportionate share of the malignant cells during even a systemically administered treatment.
  • Stem cells and healthy cells may be spared from wholesale programmed cell death, even if exposed to photoactivated agents, provided that such photoactivated agents degenerate from the excited state to a lower energy state prior to binding, mitosis or other mechanisms for creating damage to the cells of a substantial fraction of the healthy stem cells.
  • an auto-immune response may not be induced.
  • a blocking agent may be used that prevents or reduces damage to stem cells or healthy cells, selectively, which would otherwise be impaired.
  • the blocking agent is selected or is administered such that the blocking agent does not impart a similar benefit to malignant cells, for example.
  • stem cells are targeted, specifically, for destruction with the intention of replacing the stem cells with a donor cell line or previously stored, healthy cells of the patient.
  • no blocking agent is used.
  • a carrier or photosensitizer is used that specifically targets the stem cells.
  • any of the photoactivatable agents may be exposed to an excitation energy source implanted in a subject preferably near a target site.
  • the photoactive agent may be directed to a receptor site by a carrier having a strong affinity for the receptor site.
  • a “strong affinity” is preferably an affinity having an equilibrium dissociation constant, K i , at least in the nanomolar, nM, range or higher.
  • the carrier may be a polypeptide and may form a covalent bond with a photoactive agent, for example.
  • the polypeptide may be an insulin, interleukin, thymopoietin or transferrin, for example.
  • a photoactive agent may have a strong affinity for the target cell without binding to a carrier.
  • a receptor site may be any of the following: nucleic acids of nucleated blood cells, molecule receptor sites of nucleated blood cells, the antigenic sites on nucleated blood cells, epitopes, or other sites where photoactive agents are capable of destroying a targeted cell.
  • thin fiber optic lines are inserted in the subject and laser light is used to photoactivate the agents.
  • a plurality of sources for supplying electromagnetic radiation energy or energy transfer are provided by one or more molecules administered to a patient.
  • the molecules may emit stimulating radiation in the correct band of wavelength to stimulate the target structure directly or to simulate the photoactivatable agents, or the molecules may transfer energy by a resonance energy transfer or other mechanism directly to he target structure or the photoactivatable agent or indirectly by a cascade effect via other molecular interactions.
  • a delayed luminescence emission was also observed in biological systems [F.-A. Popp and Y. Yan, “Delayed luminescence of biological systems in terms of coherent states,” Phys. Lett. A 293, 93-97 (2002); A. Scordino, A. Triglia, F. Musumeci, F. Grasso, and Z. Rajfur, “Influence of the presence of Atrazine in water on in-vivo delayed luminescence of acetabularium acetabulum,” J. Photochem. Photobiol., B, 32, 11-17 (1996); This delayed luminescence was used in quality control of vegetable products [ A. Triglia, G. La Malfa, F. Musumeci, C. Leonardi, and A.
  • UV excitation can further enhance the ultra-weak emission and a method for detecting UV-A-laser-induced ultra-weak photon emission was used to evaluate differences between cancer and normal cells.
  • a method for detecting UV-A-laser-induced ultra-weak photon emission was used to evaluate differences between cancer and normal cells.
  • the initiation energy upon applying an initiation energy from at least one source to a target structure in a subject in need of treatment, the initiation energy contacts the target structure and induces a predetermined change in said target structure in situ,
  • the predetermined change is the enhancement of energy emission from the target, which then mediates, initiates or enhances a biological activity of other target structures in the subject, or of a second type of target structure (e.g., a different cell type).
  • the patient's own cells are removed and genetically modified to provide photonic emissions.
  • tumor or healthy cells may be removed, genetically modified to induce bioluminescence and may be reinserted at the site of the disease or condition to be treated.
  • the modified, bioluminescent cells may be further modified to prevent further division of the cells or division of the cells only so long as a regulating agent is present.
  • a biocompatible emitting source such as a fluorescing metal nanoparticle or fluorescing dye molecule, is selected that emits in the UV-A band.
  • the UV-A emitting source is directed to the site of a disease or condition.
  • the UV-A emitting source may be directed to the site of the disease or condition by systemically administering the UV-A emitting source.
  • the UV-A emitting source is concentrated in the target site, such as by physical insertion or by conjugating the UV-A emitting molecule with a specific carrier that is capable of concentrating the UV-A emitting source in a specific target structure, as is known in the art.
  • the UV-A emitting source is a gold nanoparticle comprising a cluster of 5 gold atoms, such as a water soluble quantum dot encapsulated by polyamidoamine dendrimers.
  • the gold atom clusters may be produced through a slow reduction of gold salts (e.g. HAuCl 4 or AuBr 3 ) or other encapsulating amines, for example.
  • gold salts e.g. HAuCl 4 or AuBr 3
  • One advantage of such a gold nanoparticle is the increased Foerster distance (i.e. R 0 ), which may be greater than 100 angstroms.
  • the equation for determining the Foerster distance is substantially different from that for molecular fluorescence, which is limited to use at distances less than 100 angstroms.
  • the gold nanoparticles are governed by nanoparticle surface to dipole equations with a 1/R 4 distance dependence rather than a 1/R 6 distance dependence.
  • this permits cytoplasmic to nuclear energy transfer between metal nanoparticles and a photoactivatable molecule, such as a psoralen and more preferably an 8-methoxypsoralen (8-MOP) administered orally to a patient, which is known to be safe and effective at inducing an apoptosis of leukocytes.
  • a UV- or light-emitting luciferase is selected as the emitting source for exciting a photoactivatable agent.
  • a luciferase may be combined with ATP or another molecule, which may then be oxygenated with additional molecules to stimulate light emission at a desired vavelength.
  • a phosphorescent emitting source may be used.
  • One advantage of a phosphorescent emitting source is that the phosphorescent emitting molecules or other source may be electroactivated or photoactivated prior to insertion into a target site either by systemic administration or direct insertion into the region of the target site. Alternatively, some of these materials can be activated, with the energy being “stored” in the activated material, until emission is stimulated by application of another energy. For example, see the discussion of U.S. Pat. No. 4,705,952 below with respect to infrared-triggered phosphors.
  • Phosphorescent materials may have longer relaxation times than fluorescent materials, because relaxation of a triplet state is subject to forbidden energy state transitions, storing the energy in the excited triplet state with only a limited number of quantum mechanical energy transfer processes available for returning to the lower energy state. Energy emission is delayed or prolonged from a fraction of a second to several hours. Otherwise, the energy emitted during phosphorescent relaxation is not otherwise different than fluorescence, and the range of wavelengths may be selected by choosing a particular phosphor.
  • nanoparticle refers to a particle having a size less than one micron. While the description of the invention describes specific examples using nanoparticles, the present invention in many embodiments is not limited to particles having a size less than one micron. However, in many of the embodiments, the size range of having a size less than one micron, and especially less than 100 nm produces properties of special interest such as for example emission lifetime luminescence quenching, luminescent quantum efficiency, and concentration quenching and such as for example diffusion, penetration, and dispersion into mediums where larger size particles would not migrate.
  • U.S. Pat. No. 4,705,952 (the contents of which are hereby incorporated herein by reference) describes an infrared-triggered phosphor that stored energy in the form of visible light of a first wavelength and released energy in the form of visible light of a second wavelength when triggered by infrared light.
  • U.S. Pat. No. 4,705,952 describes that “the upconversion continues for as long as several days before a new short recharge is required.”
  • the phosphors in U.S. Pat. No. 4,705,952 were compositions of alkaline earth metal sulfides, rare earth dopants, and fusible salts.
  • 4,705,952 were more specifically phosphors made from strontium sulfide, barium sulfide and mixtures thereof; including a dopant from the rare earth series and europium oxide, and mixtures thereof; and including a fusible salt of fluorides, chlorides, bromides, and iodides of lithium, sodium, potassium, cesium, magnesium, calcium, strontium, and barium, and mixtures thereof.
  • the materials described in U.S. Pat. No. 4,705,952 are useful in various embodiments of the invention.
  • U.S. Pat. No. 4,705,952 describes that “the storage times become extremely long, on the order of years.”
  • the material is thus adapted to receive infrared photons and to emit higher energy photons in a close to 1:1 relation.
  • these infrared-triggered phosphors can be used in various embodiments of the present invention as a viable mechanism where commercial IR lasers are used to activate phosphorescence in a medium, thereby in a patient generating visible or ultraviolet light.
  • a combined electromagnetic energy harvester molecule is designed, such as the combined light harvester disclosed in J. Am. Chem. Soc. 2005, 127, 9760-9768, the entire contents of which are hereby incorporated by reference.
  • a resonance energy transfer cascade may be used to harvest a wide band of electromagnetic radiation resulting in emission of a narrow band of fluorescent energy.
  • a further energy resonance transfer excites the photoactivatable molecule, when the photoactivatable molecule is nearby stimulated combined energy harvester molecules.
  • a Stokes shift of an emitting source or a series of emitting sources arranged in a cascade is selected to convert a shorter wavelength energy, such as X-rays, to a longer wavelength fluorescence emission such a optical or UV-A, which is used to stimulate a photoactivatable molecule at the location of the target structure.
  • the photoactivatable molecule is selected to cause the predetermined change in target structure without causing substantial harm to normal, healthy cells.
  • the photoactivatable agent can be a photocaged complex having an active agent contained within a photocage.
  • the active agent is bulked up with other molecules that prevent it from binding to specific targets, thus masking its activity.
  • the photocage complex is photoactivated, the bulk falls off, exposing the active agent.
  • the photocage molecules can be photoactive (i.e. when photoactivated, they are caused to dissociate from the photocage complex, thus exposing the active agent within), or the active agent can be the photoactivatable agent (which when photoactivated causes the photocage to fall off), or both the photocage and the active agent are photoactivated, with the same or different wavelengths.
  • a toxic chemotherapeutic agent can be photocaged, which will reduce the systemic toxicity when delivered. Once the agent is concentrated in the tumor, the agent is irradiated with an activation energy. This causes the “cage” to fall off, leaving a cytotoxic agent in the tumor cell.
  • Suitable photocages include those disclosed by Young and Deiters in “Photochemical Control of Biological Processes”, Org. Biomol. Chem., 5, pp. 999-1005 (2007) and “Photochemical Hammerhead Ribozyme Activation”, Bioorganic & Medicinal Chemistry Letters, 16(10), pp. 2658-2661 (2006), the contents of which are hereby incorporated by reference.
  • the use of light for uncaging a compound or agent is used for elucidation of neuron functions and imaging, for example, two-photon glutamine uncaging (Harvey C D, et al., Nature, 450:1195-1202 (2007); Eder M, et al., Rev. Neurosci., 15:167-183 (2004)).
  • Other signaling molecules can be released by UV light stimulation, e.g., GABA, secondary messengers (e.g., Ca2+ and Mg 2+ ), carbachol, capsaicin, and ATP (Zhang F., et al., 2006). Chemical modifications of ion channels and receptors may be carried out to render them light-responsive.
  • Ca 2+ is involved in controlling fertilization, differentiation, proliferation, apoptosis, synaptic plasticity, memory, and developing axons.
  • Ca 2+ waves can be induced by UV irradiation (single-photon absorption) and NIR irradiation (two-photon absorption) by releasing caged Ca2+, an extracellular purinergic messenger InsP3 (Braet K., et al., Cell Calcium, 33:37-48 (2003)), or ion channel ligands (Zhang F., et al., 2006).
  • a light-sensitive protein is introduced into cells or live subjects via a number of techniques including electroporation, DNA microinjection, viral delivery, liposomal transfection, creation of transgenic lines and calcium-phosphate precipitation.
  • lentiviral technology provides a convenient combination a conventional combination of stable long-term expression, ease of high-titer vector production and low immunogenicity.
  • the light-sensitive protein may be, for example, channelrhodopsin-2 (ChR2) and chloride pump halorhodopsin (NpHR).
  • the light protein encoding gene(s) along with a cell-specific promoter can be incorporated into the lentiviral vector or other vector providing delivery of the light-sensitive protein encoding gene into a target cell.
  • ChR2 containing i light sensor and a cation channel provides electrical stimulation of appropriate speed and magnitude to activate neuronal spike firing, when the cells harboring Ch2R are pulsed with light.
  • a lanthanide chelate capable of intense luminescence is used.
  • a lanthanide chelator may be covalently joined to a coumarin or coumarin derivative or a quinolone or quinolone-derivative sensitizer.
  • Sensitizers may be a 2- or 4-quinolone, a 2- or 4-coumarin, or derivatives or combinations of these examples.
  • a carbostyril 124 (7-amino-4-methyl-2-quinolone), a coumarin 120 (7-amino-4-methyl-2-coumarin), a coumarin 124 (7-amino-4-(trifluoromethyl)-2-coumarin), aminoinethyltrimethylpsoralen or other similar sensitizer may be used.
  • Chelates may be selected to form high affinity complexes with lanthanides, such as terbium or europium, through chelator groups, such as DTPA. Such chelates may be coupled to any of a wide variety of well known probes or carriers, and may be used for resonance energy transfer to a psoralen or psoralen-derivative, such as 8-MOP, or other photoactive molecules capable of binding DNA.
  • the lanthanide chelate is localized at the site of the disease using an appropriate carrier molecule, particle or polymer, and a source of electromagnetic energy is introduced by minimally invasive procedures to irradiate the target structure, after exposure to the lanthanide chelate and a photoactive molecule.
  • a biocompatible, endogenous fluorophore emitter is selected to stimulate resonance energy transfer to a photoactivatable molecule.
  • a biocompatible emitter with an emission maxima within the absorption range of the biocompatible, endogenous fluorophore emitter may be selected to stimulate an excited state in fluorophore emitter.
  • One or more halogen atoms may be added to any cyclic ring structure capable of intercalation between the stacked nucleotide bases in a nucleic acid (either DNA or RNA) to confer new photoactive properties to the intercalator.
  • any intercalating molecule may be selectively modified by halogenation or addition of non-hydrogen bonding ionic substituents to impart advantages in its reaction photochemistry and its competitive binding affinity for nucleic acids over cell membranes or charged proteins, as is known in the art.
  • Skin photosensitivity is a major toxicity of photosensitizers. Severe sunburn occurs if skin is exposed to direct sunlight for even a few minutes. Early murine research hinted at a vigorous and long term stimulation of immune response; however, actual clinical testing has failed to achieve the early promises of photodynamic therapies.
  • the early photosensitizers for photodynamic therapies targeted type II responses, which created singlet oxygen when photoactivated in the presence of oxygen. The singlet oxygen caused cellular necrosis and was associated with inflammation and an immune response. Some additional photosensitizers have been developed to induce type I responses, directly damaging cellular structures.
  • Porfimer sodium (Photofrin; QLT Therapeutics, Vancouver, BC, Canada), is a partially purified preparation of hematoporphyrin derivative (HpD).
  • Photofrin has been approved by the US Food and Drug Administration for the treatment of obstructing esophageal cancer, microinvasive endobronchial non-small cell lung cancer, and obstructing endobronchial non-small cell lung cancer.
  • Photofrin is activated with 630 nm, which has a tissue penetration of approximately 2 to 5 mm. Photofrin has a relatively long duration of skin photosensitivity (approximately 4 to 6 weeks).
  • Tetra (m-hydroxyphenyl) chlorin (Foscan; Ontario Pharmaceuticals, Stirling, UK), is a synthetic chlorine compound that is activated by 652 nm light.
  • Clinical studies have demonstrated a tissue effect of up to 10 mm with Foscan and 652 nm light.
  • Foscan is more selectively a photosensitizer in tumors than normal tissues, and requires a comparatively short light activation time.
  • a recommended dose of 0.1 mg/kg is comparatively low and comparatively low doses of light may be used. Nevertheless, duration of skin photosensitivity is reasonable (approximately 2 weeks).
  • Foscan induces a comparatively high yield of singlet oxygen, which may be the primary mechanism of DNA damage for this molecule.
  • Motexafin lutetium (Lutetium texaphryin) is activated by light in the near infared region (732 nm). Absorption at this wavelength has the advantage of potentially deeper penetration into tissues, compared with the amount of light used to activate other photosensitizers ( FIGS. 2A and 2B ). Lutetium texaphryin also has one of the greatest reported selectivities for tumors compared to selectivities of normal tissues. Young S W, et al.: Lutetium texaphyrin (PCI-0123) a near-infrared, water-soluble photosensitizer. Photochem Photobiol 1996, 63:892-897.
  • Lutetium texaphryin has been evaluated for metastatic skin cancers. It is currently under investigation for treatment of recurrent breast cancer and for locally recurrent prostate cancer. The high selectivity for tumors promises improved results in clinical trials.
  • the approach may be used with any source for the excitation of higher electronic energy states, such as electrical, chemical and/or radiation, individually or combined into a system for activating an activatable molecule.
  • the process may be a photopheresis process or may be similar to photophoresis. While photophoresis is generally thought to be limited to photonic excitation, such as by UV-light, other forms of radiation may be used as a part of a system to activate an activatable molecule.
  • Radiation includes ionizing radiation which is high energy radiation, such as an X-ray or a gamma ray, which interacts to produce ion pairs in matter.
  • Radiation also includes high linear energy transfer irradiation, low linear energy transfer irradiation, alpha rays, beta rays, neutron beams, accelerated electron beams, and ultraviolet rays. Radiation also includes proton, photon and fission-spectrum neutrons. Higher energy ionizing radiation may be combined with chemical processes to produce energy states favorable for resonance energy transfer, for example. Other combinations and variations of these sources of excitation energy may be combined as is known in the art, in order to stimulate the activation of an activatable molecule, such as 8-MOP.
  • ionizing radiation is directed at a solid tumor and stimulates, directly or indirectly, activation of 8-MOP, as well as directly damaging the DNA of malignant tumor cells. In this example, either the effect of ionizing radiation or the photophoresis-like activation of 8-MOP may be thought of as an adjuvant therapy to the other.
  • the present invention provides a method for treating a condition, disease or disorder mediated by a target structure in a subject, comprising:
  • the present invention provides a method for treating a condition, disease or disorder mediated by a target structure in a subject, comprising:
  • the one or more energy modulation agents convert the initiation energy applied to UV-A or visible energy, which then activates the activatable agent in situ
  • occurrence of the predetermined change causes an increase in rate or decrease in rate of cell division and/or growth to treat the condition, disease or disorder.
  • the activatable pharmaceutical agent can be activated by a single or multiphoton absorption event.
  • the amount of singlet oxygen required to cause cell lysis, and thus cell death is 0.32 ⁇ 10 ⁇ 3 mol/liter or more, or 10 9 singlet oxygen molecules/cell or more.
  • the level of singlet oxygen production caused by the initiation energy used or activatable pharmaceutical agent upon activation be less than level needed to cause cell lysis.
  • the activatablc pharmaceutical agent preferably a photoactive agent
  • a carrier having a strong affinity for the receptor site.
  • the carrier may be a polypeptide and may form a covalent bond with a photo active agent, for example.
  • the polypeptide may be an insulin, interleukin, thymopoietin or transferrin, for example.
  • a photoactive pharmaceutical agent may have a strong affinity for the target cell without a binding to a carrier.
  • a treatment may be applied that acts to slow or pause mitosis.
  • Such a treatment is capable of slowing the division of rapidly dividing healthy cells or stem cells without pausing mitosis of cancerous cells.
  • the difference in growth rate between the non-target cells and target cells are further differentiated to enhance the effectiveness of the methods of the present invention.
  • methods in accordance with the present invention may further include adding an additive to alleviate treatment side-effects.
  • additives may include, but are not limited to, antioxidants, adjuvant, or combinations thereof.
  • psoralen is used as the activatable pharmaceutical agent
  • UV-A is used as the activating energy
  • antioxidants are added to reduce the unwanted side-effects of irradiation.
  • the present invention also provides methods for producing an autovaccine, including: (1) providing a population of targeted cells; (2) treating the cells ex vivo with a psoralen or a derivative thereof; (3) activating the psoralen with an initiation energy source to induce a predetermined change in a target structure in the population of the target cells; and (4) returning the treated cells back to the host to induce an autovaccine effect against the targeted cell, wherein the treated cells cause an autovaccine effect.
  • a method for generating an autovaccine for a subject comprises:
  • methods in accordance with the present invention may further include a method for modifying a target structure which mediates or is associated with a biological activity, comprising:
  • the predetermined change enhances the expression of, promotes the growth of, or increases the quantity of said target structure; enhances, inhibits or stabilizes the usual biological activity of said target structure compared to a similar untreated target structure, and/or alters the immunological or chemical properties of said target structure.
  • said target structure is a compound that is modified by said predetermined change to be more or less antigenic or immunogenic
  • the activatable pharmaceutical agent and derivatives thereof as well as the energy modulation agent can be incorporated into pharmaceutical compositions suitable for administration.
  • Such compositions typically comprise the activatable pharmaceutical agent and a pharmaceutically acceptable carrier.
  • the pharmaceutical composition also comprises at least one additive having a complementary therapeutic or diagnostic effect, wherein the additive is one selected from an antioxidant, an adjuvant, or a combination thereof.
  • “pharmaceutically acceptable carrier” is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration.
  • the use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use thereof in the compositions is contemplated.
  • Supplementary active compounds can also be incorporated into the compositions. Modifications can be made to the compound of the present invention to affect solubility or clearance of the compound. These molecules may also be synthesized with D-amino acids to increase resistance to enzymatic degradation. If necessary, the activatable pharmaceutical agent can be co-administered with a solubilizing agent, such as cyclodextran.
  • a pharmaceutical composition of the invention is formulated to be compatible with its intended route of administration.
  • routes of administration include parenteral, e.g., intravenous, intradermal, subcutaneous, oral (e.g., inhalation), transdermal (topical), transmucosal, rectal administration, and direct injection into the affected area, such as direct injection into a tumor
  • Solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerin, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates, and agents for the adjustment of tonicity such as sodium chloride or dextrose.
  • compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion.
  • suitable carriers include physiological saline, bacteriostatic water, or phosphate buffered saline (PBS).
  • PBS phosphate buffered saline
  • the composition must be sterile and should be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi.
  • the carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof.
  • the proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants.
  • Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like.
  • isotonic agents for example, sugars, polyalcohols such as manitol, sorbitol, sodium chloride in the composition.
  • Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate and gelatin.
  • Sterile injectable solutions can be prepared by incorporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization.
  • dispersions are prepared by incorporating the active compound into a sterile vehicle that contains a basic dispersion medium and the required other ingredients from those enumerated above.
  • methods of preparation are vacuum drying and freeze-drying that yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
  • Oral compositions generally include an inert diluent or an edible carrier. They can be enclosed in gelatin capsules or compressed into tablets. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules. Oral compositions can also be prepared using a fluid carrier for use as a mouthwash, wherein the compound in the fluid carrier is applied orally and swished and expectorated or swallowed. Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition.
  • the tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring.
  • a binder such as microcrystalline cellulose, gum tragacanth or gelatin
  • an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch
  • a lubricant such as magnesium stearate or Sterotes
  • a glidant such as colloidal silicon dioxide
  • the compounds are delivered in the form of an aerosol spray from pressured container or dispenser which contains a suitable propellant, e.g., a gas such as carbon dioxide, or a nebulizer.
  • a suitable propellant e.g., a gas such as carbon dioxide, or a nebulizer.
  • Systemic administration can also be by transmucosal or transdermal means.
  • penetrants appropriate to the barrier to be permeated are used in the formulation.
  • penetrants are generally known in the art, and include, for example, for transmucosal administration, detergents, bile salts, and fusidic acid derivatives.
  • Transmucosal administration can be accomplished through the use of nasal sprays or suppositories.
  • the active compounds are formulated into ointments, salves, gels, or creams as generally known in the art.
  • the compounds can also be prepared in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery.
  • suppositories e.g., with conventional suppository bases such as cocoa butter and other glycerides
  • retention enemas for rectal delivery.
  • the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems.
  • a controlled release formulation including implants and microencapsulated delivery systems.
  • Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. The materials can also be obtained commercially.
  • Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically acceptable carriers. These can be prepared according to methods known to those skilled in the art, for example, as described in U.S. Pat. No. 4,522,811.
  • Dosage unit form refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.
  • the specification for the dosage unit forms of the invention are dictated by and directly dependent on the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active compound for the treatment of individuals.
  • compositions can be included in a container, pack, or dispenser together with instructions for administration.
  • Methods of administering agents according to the present invention are not limited to the conventional means such as injection or oral infusion, but include more advanced and complex forms of energy transfer.
  • genetically engineered cells that carry and express energy modulation agents may be used.
  • Cells from the host may be transfected with genetically engineered vectors that express bioluminescent agents.
  • Transfection may be accomplished via in situ gene therapy techniques such as injection of viral vectors or gene guns, or may be performed ex vivo by removing a sample of the host's cells and then returning to the host upon successful transfection.
  • tile initiation energy source may be a biochemical source as such ATP, in which case the initiation energy source is considered to be directly implanted in the transfected cell.
  • a conventional micro-emitter device capable of acting as an initiation energy source may be transplanted at the site of the diseased cells.
  • the order of administering the different agents is not particularly limited.
  • the activatable pharmaceutical agent may be administered before the energy modulation agent, while in other embodiments the energy modulation agent may be administered prior to the activatable pharmaceutical agent.
  • different combinations of ordering may be advantageously employed depending on factors such as the absorption rate of the agents, the localization and molecular trafficking properties of the agents, and other pharmacokinetics or pharmacodynamics considerations.
  • a further embodiment is the use of the present invention for the treatment of skin cancer.
  • a photoactivatable agent preferably psoralen
  • psoralen is given to the patient, and is delivered to the skin lesion via the blood supply.
  • An activation source having limited penetration ability such as UV or IR
  • UV or IR is shined directly on the skin—in the case of psoralen, it would be a UV light, or an IR source.
  • an IR source With the use of an IR source, the irradiation would penetrate deeper and generate UV via two single photon events with psoralen.
  • methods according to this aspect of the present invention further include a step of separating the components of the treated cells into fractions and testing each fraction for autovaccine effect in a host.
  • the components thus isolated and identified may then serve as an effective autovaccine to stimulate the host's immune system to suppress growth of the targeted cells.
  • the present invention further provides systems and kits for practicing the above described methods.
  • a system for producing an auto-vaccine in a subject comprises:
  • a system in accordance with the present invention may include: (1) an initiation energy source; and (2) one or more energy modulation agents.
  • the system may further comprise (3) one or more activatable pharmaceutical agents.
  • the system may comprise only (1) the initiation energy source.
  • the system may comprise (1) an initiation energy source; and (3) one or more activatable pharmaceutical agents.
  • FIG. 3 illustrates a system according to one exemplary embodiment of the present invention. Referring to FIG. 3 , an exemplary system according to one embodiment of the present invention may have an initiation energy source 1 directed at the subject 4 . An activatable pharmaceutical agent 2 and an energy modulation agent 3 are administered to the subject 4 .
  • the initiation energy source may additionally be controlled by a computer system 5 that is capable of directing the delivery of the initiation energy.
  • the initiation energy source may be a linear accelerator equipped with image guided computer-control capability to deliver a precisely calibrated beam of radiation to a pre-selected coordinate.
  • linear accelerators are the SmartBeamTM IMRT (intensity modulated radiation therapy) system from Varian medical systems (Varian Medical Systems, Inc., Palo Alto, Calif.).
  • endoscopic or laproscopic devices equipped with appropriate initiation energy emitter may be used as the initiation energy source.
  • the initiation energy may be navigated and positioned at the pre-selected coordinate to deliver the desired amount of initiation energy to the site.
  • dose calculation and robotic manipulation devices may also be included in the system.
  • a computer implemented system for designing and selecting suitable combinations of initiation energy source, energy transfer agent, and activatable pharmaceutical agent comprising:
  • CPU central processing unit
  • system upon selection of a target cellular structure or component, computes an excitable compound that is capable of binding with the target structure followed by a computation to predict the resonance absorption energy of the excitable compound.
  • FIG. 4 illustrates an exemplary computer implemented system according to this embodiment of the present invention.
  • an exemplary computer-implemented system may have a central processing unit (CPU) connected to a memory unit, configured such that the CPU is capable of processing user inputs and selecting a combination of initiation source, activatable pharmaceutical agent, and energy transfer agent based on an energy spectrum comparison for use in a method of the present invention.
  • CPU central processing unit
  • memory unit configured such that the CPU is capable of processing user inputs and selecting a combination of initiation source, activatable pharmaceutical agent, and energy transfer agent based on an energy spectrum comparison for use in a method of the present invention.
  • FIG. 5 illustrates a computer system 1201 for implementing various embodiments of the present invention.
  • the computer system 1201 may be used as the controller 55 to perform any or all of the functions of the CPU described above.
  • the computer system 1201 includes a bus 1202 or other communication mechanism for communicating information, and a processor 1203 coupled with the bus 1202 for processing the information.
  • the computer system 1201 also includes a main memory 1204 , such as a random access memory (RAM) or other dynamic storage device (e.g., dynamic RAM (DRAM), static RAM (SRAM), and synchronous DRAM (SDRAM)), coupled to the bus 1202 for storing information arid instructions to be executed by processor 1203 .
  • RAM random access memory
  • DRAM dynamic RAM
  • SRAM static RAM
  • SDRAM synchronous DRAM
  • the main memory 1204 may be used for storing temporary variables or other intermediate information during the execution of instructions by the processor 1203 .
  • the computer system 1201 further includes a read only memory (ROM) 1205 or other static storage device (e.g., programmable ROM (PROM), erasable PROM (EPROM), and electrically erasable PROM (EEPROM)) coupled to the bus 1202 for storing static informal:ion and instructions for the processor 1203 .
  • ROM read only memory
  • PROM programmable ROM
  • EPROM erasable PROM
  • EEPROM electrically erasable PROM
  • the computer system 1201 also includes a disk controller 1206 coupled to the bus 1202 to control one or more storage devices for storing information and instructions, such as a magnetic hard disk 1207 , and a removable media drive 1208 (e.g., floppy disk drive, read-only compact disc drive, read/write compact disc drive, compact disc jukebox, tape drive, and removable magneto-optical drive).
  • a removable media drive 1208 e.g., floppy disk drive, read-only compact disc drive, read/write compact disc drive, compact disc jukebox, tape drive, and removable magneto-optical drive.
  • the storage devices may be added to the computer system 1201 using an appropriate device interface (e.g., small computer system interface (SCSI), integrated device electronics (IDE), enhanced-IDE (E-IDE), direct memory access (DMA), or ultra-DMA).
  • SCSI small computer system interface
  • IDE integrated device electronics
  • E-IDE enhanced-IDE
  • DMA direct memory access
  • ultra-DMA ultra-DMA
  • the computer system 1201 may also include special purpose logic devices (e.g., application specific integrated circuits (ASICs)) or configurable logic devices (e.g., simple programmable logic devices (SPLDs), complex programmable logic devices (CPLDs), and field programmable gate arrays (FPGAs)).
  • ASICs application specific integrated circuits
  • SPLDs simple programmable logic devices
  • CPLDs complex programmable logic devices
  • FPGAs field programmable gate arrays
  • the computer system 1201 may also include a display controller 1209 coupled to the bus 1202 to control a display 1210 , such as a cathode ray tube (CRT), for displaying information to a computer user.
  • the computer system includes input devices, such as a keyboard 1211 and a pointing device 1212 , for interacting with a computer user and providing information to the processor 1203 .
  • the pointing device 1212 may be a mouse, a trackball, or a pointing stick for communicating direction information and command selections to the processor 1203 and for controlling cursor movement on the display 1210 .
  • a printer may provide printed listings of data stored and/or generated by the computer system 1201 .
  • the computer system 1201 performs a portion or all of the processing steps of the invention (such as for example those described in relation to FIG. 5 ) in response to the processor 1203 executing one or more sequences of one or more instructions contained in a memory, such as the main memory 1204 .
  • a memory such as the main memory 1204 .
  • Such instructions may be read into the main memory 1204 from another computer readable medium, such as a hard disk 1207 or a removable media drive 1208 .
  • processors in a multi-processing arrangement may also be employed to execute the sequences of instructions contained in main memory 1204 .
  • hard-wired circuitry may be used in place of or in combination with software instructions. Thus, embodiments are not limited to any specific combination of hardware circuitry and software.
  • the computer system 1201 includes at least one computer readable medium or memory for holding instructions programmed according to the teachings of the invention and for containing data structures, tables, records, or other data described herein.
  • Examples of computer readable media are compact discs, hard disks, floppy disks, tape, magneto-optical disks, PROMs (EPROM, EEPROM, flash EPROM), DRAM, SRAM, SDRAM, or any other magnetic medium, compact discs (e.g., CD-ROM), or any other optical medium, punch cards, paper tape, or other physical medium with patterns of holes, a carrier wave (described below), or any other medium from which a computer can read.
  • the present invention includes software for controlling the computer system 1201 , for driving a device or devices for implementing the invention, and for enabling the computer system 1201 to interact with a human user (e.g., print production personnel).
  • software may include, but is not limited to, device drivers, operating systems, development tools, and applications software.
  • Such computer readable media further includes the computer program product of the present invention for performing all or a portion (if processing is distributed) of the processing performed in implementing the invention.
  • the computer code devices of the present invention may be any interpretable or executable code mechanism, including but not limited to scripts, interpretable programs, dynamic link libraries (DLLs), Java classes, and complete executable programs. Moreover, parts of the processing of the present invention may be distributed for better performance, reliability, and/or cost.
  • Non-volatile media includes, for example, optical, magnetic disks, and magneto-optical disks, such as the hard disk 1207 or the removable media drive 1208 .
  • Volatile media includes dynamic memory, such as the main memory 1204 .
  • Transmission media includes coaxial cables, copper wire and fiber optics, including the wires that make up the bus 1202 . Transmission media also may also take the form of acoustic or light waves, such as those generated during radio wave and infrared data communications.
  • Various forms of computer readable media may be involved in carrying out one or more sequences of one or more instructions to processor 1203 for execution.
  • the instructions may initially be carried on a magnetic disk of a remote computer.
  • the remote computer can load the instructions for implementing all or a portion of the present invention remotely into a dynamic memory and send the instructions over a telephone line using a modem.
  • a modem local to the computer system 1201 may receive the data on the telephone line and use an infrared transmitter to convert the data to an infrared signal.
  • An infrared detector coupled to the bus 1202 can receive the data carried in the infrared signal and place the data on the bus 1202 .
  • the bus 1202 carries the data to the main memory 1204 , from which the processor 1203 retrieves and executes the instructions.
  • the instructions received by the main memory 1204 may optionally be stored on storage device 1207 or 1208 either before or after execution by processor 1203 .
  • the computer system 1201 also includes a communication interface 1213 coupled to the bus 1202 .
  • the communication interface 1213 provides a two-way data communication coupling to a network link 1214 that is connected to, for example, a local area network (LAN) 1215 , or to another communications network 1216 such as the Internet.
  • LAN local area network
  • the communication interface 1213 may be a network interface card to attach to any packet switched LAN.
  • the communication interface 1213 may be an asymmetrical digital subscriber line (ADSL) card, an integrated services digital network (ISDN) card or a modem to provide a data communication connection to a corresponding type of communications line.
  • Wireless links may also be implemented.
  • the communication interface 1213 sends and receives electrical, electromagnetic or optical signals that carry digital data streams representing various types of information.
  • the network link 1214 typically provides data communication through one or more networks to other data devices.
  • the network link 1214 may provide a connection to another computer through a local network 1215 (e.g., a LAN) or through equipment operated by a service provider, which provides communication services through a communications network 1216 .
  • the local network 1214 and the communications network 1216 use, for example, electrical, electromagnetic, or optical signals that carry digital data streams, and the associated physical layer (e.g., CAT 5 cable, coaxial cable, optical fiber, etc).
  • the signals through the various networks and the signals on the network link 1214 and through the communication interface 1213 , which carry the digital data to and from the computer system 1201 maybe implemented in baseband signals, or carrier wave based signals.
  • the baseband signals convey the digital data as unmodulated electrical pulses that are descriptive of a stream of digital data bits, where the term “bits” is to be construed broadly to mean symbol, where each symbol conveys at least one or more information bits.
  • the digital data may also be used to modulate a carrier wave, such as with amplitude, phase and/or frequency shift keyed signals that are propagated over a conductive media, or transmitted as electromagnetic waves through a propagation medium.
  • the digital data may be sent as unmodulated baseband data through a “wired” communication channel and/or sent within a predetermined frequency band, different than baseband, by modulating a carrier wave.
  • the computer system 1201 can transmit and receive data, including program code, through the network(s) 1215 and 1216 , the network link 1214 , and the communication interface 1213 .
  • the network link 1214 may provide a connection through a LAN 1215 to a mobile device 1217 such as a personal digital assistant (PDA) laptop computer, or cellular telephone.
  • PDA personal digital assistant
  • the exemplary energy spectrum previously noted in FIG. 1 may also be used in this computer-implemented system.
  • kits to facilitate application of the present invention.
  • a kit including a psoralen, and fractionating containers for easy fractionation and isolation of autovaccines is contemplated.
  • a further embodiment of kit would comprise at least one activatable pharmaceutical agent capable of causing a predetermined cellular change, at least one energy modulation agent capable of activating the at least one activatable agent when energized, and containers suitable for storing the agents in stable form, and preferably further comprising instructions for administering the at least one activatable pharmaceutical agent and at least one energy modulation agent to a subject, and for applying an initiation energy from an initiation energy source to activate the activatable pharmaceutical agent.
  • the instructions could be in any desired form, including but not limited to, printed on a kit insert, printed on one or more containers, as well as electronically stored instructions provided on an electronic storage medium, such as a computer readable storage medium. Also optionally included is a software package on a computer readable storage medium that permits the user to integrate the information and calculate a control dose, to calculate and control intensity of the irradiation source.
  • a kit for modifying a target structure which mediates or is associated with a biological activity comprising:
  • kits for performing a condition, disorder or disease treatment comprises:
  • At least one energy modulation agent capable of adsorbing, intensifying or modifying an initiation energy into an energy that is capable of causing a predetermined change in a target structure
  • the kit may further comprise instructions for administering the at least one energy modulation agent to a subject.
  • the present invention is significantly different from the phototherapy technique often referred to Photo-thermal Therapy (PTT).
  • PTT Photo-thermal Therapy
  • PST photospectral therapy
  • the light absorbing species include natural chromophores in tissue or exogenous dye compounds such as indocyanine green, naphthalocyanines, and porphyrins coordinated with transition metals and metallic nanoparticles and nanoshells of metals. Natural chromophores, however, suffer from very low absorption.
  • the choice of the exogenous photothermal agents is made on the basis of their strong absorption cross sections and highly efficient light-to-heat conversion. This feature greatly minimizes the amount of laser energy needed to induce local damage of the diseased cells, making the therapy method less invasive.
  • nanoparticles such as gold nanoparticles and nanoshells have recently been used.
  • the promising role of nanoshells in photothermal therapy of tumors has been demonstrated [Hirsch, L. R., Stafford, R. J., Bankson, J. A., Sershen, S. R., Rivera, B., Price, R. E., Hazle, J. D., Halas, N. J., and West, J. L., Nanoshell - mediated near - infrared thermal therapy of tumors under magnetic resonance guidance. PNAS, 2003. 100(23): p. 13549-13554].
  • the PST method of the present invention is based on the radiative processes (fluorescence, phosphorescence, luminescence, Raman, etc) whereas the PTT method is based on the radiationless processes (IC, VR and heat conversion) in molecules.
  • PEPST the plasmonics-enhanced spectroscopic properties (spectral absorption, emission, scattering) are the major factors involved in the treatment.
  • the PEPST principle is based on the enhancement mechanisms of the electromagnetic field effect.
  • electromagnetic enhancement There are two main sources of electromagnetic enhancement: (1) first, the laser electromagnetic field is enhanced due to the addition of a field caused by the polarization of the metal particle; (2) in addition to the enhancement of the excitation laser field, there is also another enhancement due to the molecule radiating an amplified emission (luminescence, Raman, etc.) field, which further polarizes the metal particle, thereby acting as an antenna to further amplify the Raman/Luminescence signal.
  • Electromagnetic enhancements are divided into two main classes: a) enhancements that occur only in the presence of a radiation field, and b) enhancements that occur even without a radiation field.
  • the first class of enhancements is further divided into several processes.
  • Plasma resonances on the substrate surfaces, also called surface plasmons, provide a major contribution to electromagnetic enhancement.
  • An effective type of plasmonics-active substrate comprises nanostructured metal particles, protrusions, or rough surfaces of metallic materials. Incident light irradiating these surfaces excites conduction electrons in the metal, and induces excitation of surface plasmons leading to Raman/luminescence enhancement.
  • the metal nanoparticles (or nanostructured roughness) become polarized, resulting in large field-induced polarizations and thus large local fields on the surface.
  • These local fields increase the luminescence/Raman emission intensity, which is proportional to the square of the applied field at the molecule.
  • the effective electromagnetic field experienced by the analyte molecule on these surfaces is much larger than the actual applied field. This field decreases as 1/r 3 away from the surface. Therefore, in the electromagnetic models, the luminescence/Raman-active analyte molecule is not required to be in contact with the metallic surface but can be located anywhere within the range of the enhanced local field, which can polarize this molecule.
  • the dipole oscillating at the wavelength X of Raman or luminescence can, in turn, polarize the metallic nanostructures and, if X is in resonance with the localized surface plasmons, the nanostructures can enhance the observed emission light (Raman or luminescence).
  • the laser electromagnetic field is enhanced due to the addition of a field caused by the polarization of the metal particle; (2) in addition to the enhancement of the excitation laser field, there is also another enhancement due to the molecule radiating an amplified Raman/luminescence field, which further polarizes the metal particle, thereby acting as an antenna to further amplify the Raman/luminescence signal.
  • Plasmonics-active metal nanoparticles also exhibit strongly enhanced visible and near-infrared light absorption, several orders of magnitude more intense compared to conventional laser phototherapy agents.
  • the use of plasmonic nanoparticles as highly enhanced photoabsorbing agents thus provides a selective and efficient phototherapy strategy.
  • the tunability of the spectral properties of the metal nanoparticles and the biotargeting abilities of the plasmonic nanostructures make the PEPST method promising.
  • the present invention PEPST is based on several important mechanisms:
  • SERS surface-enhanced Raman scattering
  • FIG. 6 shows the early work by Kerker modeling electromagnetic field enhancements for spherical silver nanoparticles and metallic nanoshells around dielectric cores as far back as 1984 [M. M. Kerker, Acc. Chem. Res., 17, 370 (1984)].
  • This figure shows the result of theoretical calculations of electromagnetic enhancements for isolated spherical nanospheres and nanoshells at different excitation wavelengths.
  • the intensity of the normally weak Raman scattering process is increased by factors as large as 10 13 or 10 15 for compounds adsorbed onto a SERS substrate, allowing for single-molecule detection.
  • the electromagnetic field enhancements produced near nanostructured metal surfaces nanoparticles have found increased use as fluorescence and Raman nanoprobes.
  • electromagnetic fields can be locally amplified when light is incident on metal nanostructures. These field enhancements can be quite large (typically 10 6 - to 10 7 -fold, but up to 10 15 -fold enhancement at “hot spots”).
  • an electromagnetic field e.g., a laser beam
  • electrons within the conduction band begin to oscillate at a frequency equal to that of the incident light.
  • surface plasmons produce a secondary electric field which adds to the incident field.
  • FIG. 7 shows a number of the various embodiments of PEPST probes that can be designed:
  • FIG. 7A A basic embodiment of the PEPST probe is shown in FIG. 7A .
  • This probe comprises PA molecules bound to a metal (e.g., gold) nanoparticle.
  • FIG. 8 illustrates the plasmonics-enhancement effect of the PEPST probe.
  • the gold nanoparticles can serve as a drug delivery platform.
  • Gold nanoparticles have been described as a novel technology in the field of particle-based tumor-targeted drug delivery [Giulio F. Paciotti and Lonnie Myer, David Weinreich, Dan Goia, Hoffmanvier, Richard E. McLaughlin, Lawrence Tamarkin, “ Colloidal Gold: A Novel Nanoparticle Vector for Tumor Directed Drug Delivery, Drug Delivery, 11:169-183, 2004].
  • Particle delivery systems capable of escaping phagocytic clearance by the reticuloendothelial system (RES) can facilitate targeting cancer therapeutics to solid tumors. Such delivery systems could preferentially accumulate within the tumor microenvironment under ideal conditions.
  • a particle delivery system capable of sequestering a phototherapeutic drug selectively within a tumor may also reduce the accumulation of the drug in healthy organs. Consequently, these delivery systems may increase the relative efficacy or safety of therapy (less radiation energy and intensity), and therefore, will increase the drug's therapeutic efficiency.
  • PA drug molecules e.g., aminolevulinic acid (ALA), porphyrins
  • PA drug molecules e.g., aminolevulinic acid (ALA), porphyrins
  • PDT drug ALA light of a HeNe laser (632.8-nm excitation) can be used for excitation.
  • the metal nanoparticles are designed to exhibit strong plasmon resonance band around 632.8 nm.
  • the surface plasmon resonance effect amplifies the excitation light at the nanoparticles, resulting in increased photoactivation of the PA drug molecules and improved therapy efficiency.
  • the plasmonics-enhanced mechanism can also be used with the other PEPST probes in FIGS. 7B , 7 C, 7 D, 7 E, 7 F and 7 G.
  • FIG. 34 shows yet other embodiment of plasmonics photo-active probes.
  • FIG. 35 shows yet other embodiment of plasmonics photo-active probes that have a dielectric layer between the metal and the UC materials.
  • a method for treating a condition, disorder or disease in accordance with the present invention comprises:
  • a method in accordance with the present invention comprises:
  • At least one energy modulation agent and/or excitation-generating energy modulation agent material may be also added.
  • the energy modulation agent or excitation-generating energy modulation agent material may adsorb, intensify or modify the initiation energy which is then enhanced by at least one plasmonic agent.
  • the energy modulation agent or excitation-generating energy modulation agent material may adsorb, intensify or modify energy enhanced by the at least plasmonics-active agent and emit an energy that is capable to activate the pharmaceutical activatable agent.
  • the predetermined change enhances the expression of, promotes the growth of, or increases the quantity of said target structure.
  • the predetermined change enhances, inhibits or stabilizes the usual biological activity of said target structure compared to a similar untreated target structure.
  • the predetermined change alters the immunological or chemical properties of said target structure.
  • the target structure is a compound that is modified by said predetermined change to be more or less antigenic or immunogenic.
  • the plasmonic response of nanoparticles have played a role in a growing number of applications, including surface-enhanced Raman scattering (SERS), chemical sensing, drug delivery, photothermal cancer therapy and new photonic devices.
  • SERS surface-enhanced Raman scattering
  • the investigation and application of plasmonics nanosubstrates for SERS detection has been used by one of the present inventors for over two decades [T. Vo-Dinh, “Surface-Enhanced Raman Spectroscopy Using Metallic Nanostructures,” Trends in Anal. Chem., 17,557 (1998)].
  • the substrates involve nanoparticles and semi-nanoshells comprising a layer of nanoparticles coated by a metal (such as silver) on one side (nanocaps or half-shells).
  • these shells typically comprise a metallic layer over a dielectric core.
  • these shells comprise spheroidal shells, since the plasmon resonances (both longitudinal and transverse modes) are influenced by both shell thickness and aspect ratio.
  • the present invention also includes prolate and oblate spheroidal shells, which show some interesting qualitative features in their plasmon resonances.
  • the spheroidal shell presents two degrees of freedom for tuning: the shell thickness and the shell aspect ratio [S. J. Norton and T. Vo-Dinh, “ Plasmonic Resonances of Nanoshells of Spheroidal Shape”, IEEE Trans. Nanotechnology, 6, 627-638 (2007)].
  • FIG. 9 shows some of the various embodiments of plasmonics-active nanostructures that can be designed, and are preferred embodiments of the present invention:
  • the PA drug molecules can be incorporated into a material (e.g., biocompatible polymer) that can form a nanocap onto the metal (gold) nanoparticles.
  • a material e.g., biocompatible polymer
  • the material can be a gel or biocompatible polymer that can have long-term continuous drug release properties.
  • Suitable gel or biocompatible polymers include, but are not limited to poly(esters) based on polylactide (PLA), polyglycolide (PGA), polycarpolactone (PCL), and their copolymers, as well as poly(hydroxyalkanoate)s of the PHB-PHV class, additional poly(ester)s, natural polymers, particularly, modified poly(saccharide)s, e.g., starch, cellulose, and chitosan, polyethylene oxides, poly(ether)(ester) block copolymers, and ethylene vinyl acetate copolymers.
  • the drug release mechanism can also be triggered by non-invasive techniques, such as RF, MW, ultrasound, photon ( FIG. 10 ), FIG.
  • FIG. 11 shows other possible embodiments where the PA drug molecule is bound to the metal nanoparticles via a linker that can be cut by a photon radiation.
  • a linker includes, but is not limited to, a biochemical bond ( FIG. 11A ), a DNA bond ( FIG. 11B ), or an antibody-antigen bond ( FIG. 11C ).
  • the linker is a chemically labile bond that will be broken by the chemical environment inside the cell.
  • Aggregation of metal (such as silver or gold) nanoparticles is often a problem, especially with citrate-capped gold nanospheres, cetyl trimethylammonium bromide (CTAB)-capped gold nanospheres and nanorods and nanoshells because they have poor stability when they are dispersed in buffer solution due to the aggregating effect of salt ions.
  • CAB cetyl trimethylammonium bromide
  • the biocompatibility can be improved and nanoparticle aggregation prevented by capping the nanoparticles with polyethylene glycol (PEG) (by conjugation of thiol-functionalized PEG with metal nanoparticles).
  • EPR enhanced permeability and retention effect
  • Blood vessels in tumor tissue are more “leaky” than in normal tissue, and as a result, particles, or large macromolecular species or polymeric species preferentially extravasate into tumor tissue. Particles and large molecules tend to stay a longer time in tumor tissue due to the decreased lymphatic system, whereas they are rapidly cleared out in normal tissue.
  • This tumor targeting strategy is often referred to as passive targeting whereas the antibody-targeting strategy is called active targeting.
  • the drug systems of the present invention can be bound to a bioreceptor (e.g., antibody, synthetic molecular imprint systems, DNA, proteins, lipids, cell-surface receptors, aptamers, etc.).
  • a bioreceptor e.g., antibody, synthetic molecular imprint systems, DNA, proteins, lipids, cell-surface receptors, aptamers, etc.
  • Immunotargeting modalities to deliver PA agents selectively to the diseased cells and tissue provide efficient strategies to achieving specificity, minimizing nonspecific injury to healthy cells, and reducing the radiation intensity used.
  • Biofunctionalization of metal nanoparticles e.g., gold, silver
  • nanoparticles conjugated to antibodies that recognize biomarkers specific to the diseased cells there are several targeting strategies that can be used in the present invention: (a) nanoparticles conjugated to antibodies that recognize biomarkers specific to the diseased cells; (b) nanoparticles passivated by poly (ethylene) glycol (PEG), which is used to increase the biocompatibility and biostability of nanoparticles and impart them an increased blood retention time.
  • PEG poly (ethylene) glycol
  • Bioreceptors are the key to specificity for targeting disease cells, mutated genes or specific biomarkers. They are responsible for binding the biotarget of interest to the drug system for therapy. These bioreceptors can take many forms and the different bioreceptors that have been used are as numerous as the different analytes that have been monitored using biosensors. However, bioreceptors can generally be classified into five different major categories. These categories include: 1) antibody/antigen, 2) enzymes, 3) nucleic acids/DNA, 4) cellular structures/cells and 5) biomimetic.
  • FIG. 12 illustrates a number of embodiments of the various PEPST probes with bioreceptors that can be designed. The probes are similar to those in FIG. 2 but have also a bioreceptor for tumor targeting.
  • Antibody Probes Antibody based targeting is highly active, specific and efficient.
  • the antibodies are selected to target a specific tumor marker (e.g., anti-epidermal growth factor receptor (EGFR) antibodies targeted against overexpressed EGFR on oral and cervical cancer cells; anti-Her2 antibodies against overexpressed Her2 on breast cancer cells)
  • EGFR anti-epidermal growth factor receptor
  • Antibodies are biological molecules that exhibit very specific binding capabilities for specific structures. This is very important due to the complex nature of most biological systems.
  • An antibody is a complex biomolecule, made up of hundreds of individual amino acids arranged in a highly ordered sequence. For an immune response to be produced against a particular molecule, a certain molecular size and complexity are necessary: proteins with molecular weights greater then 5000 Da are generally immunogenic.
  • an antigen and its antigen-specific antibody interact may be understood as analogous to a lock and key fit, by which specific geometrical configurations of a unique key enables it to open a lock.
  • an antigen-specific antibody “fits” its unique antigen in a highly specific manner. This unique property of antibodies is the key to their usefulness in immunosensors where only the specific analyte of interest, the antigen, fits into the antibody binding site.
  • DNA Probes The operation of gene probes is based on the hybridization process. Hybridization involves the joining of a single strand of nucleic acid with a complementary probe sequence. Hybridization of a nucleic acid probe to DNA biotargets (e.g., gene sequences of a mutation, etc) offers a very high degree of accuracy for identifying DNA sequences complementary to that of the probe. Nucleic acid strands tend to be paired to their complements in the corresponding double-stranded structure. Therefore, a single-stranded DNA molecule will seek out its complement in a complex mixture of DNA containing large numbers of other nucleic acid molecules. Hence, nucleic acid probe (i.e., gene probe) detection methods are very specific to DNA sequences. Factors affecting the hybridization or reassociation of two complementary DNA strands include temperature, contact time, salt concentration, and the degree of mismatch between the base pairs, and the length and concentration of the target and probe sequences.
  • Bioly active DNA probes can be directly or indirectly immobilized onto a drug system, such as the energy modulation agent system (e.g., gold nanoparticle, a semiconductor, quantum dot, a glass/quartz nanoparticles, etc.) surface to ensure optimal contact and maximum binding.
  • the energy modulation agent system e.g., gold nanoparticle, a semiconductor, quantum dot, a glass/quartz nanoparticles, etc.
  • the gene probes are stabilized and, therefore, can be reused repetitively.
  • Several methods can be used to bind DNA to different supports. The method commonly used for binding DNA to glass involves silanization of the glass surface followed by activation with carbodiimide or glutaraldehyde.
  • silanization methods have been used for binding to glass surfaces using 3 glycidoxypropyltrimethoxysilane (GOP) or aminopropyltrimethoxysilane (APTS), followed by covalently linking DNA via amino linkers incorporated either at the 3′ or 5′ end of the molecule during DNA synthesis.
  • GOP glycidoxypropyltrimethoxysilane
  • APTS aminopropyltrimethoxysilane
  • Enzyme Probes Enzymes are often chosen as bioreceptors based on their specific binding capabilities as well as their catalytic activity. In biocatalytic recognition mechanisms, the detection is amplified by a reaction catalyzed by macromolecules called biocatalysts. With the exception of a small group of catalytic ribonucleic acid molecules, all enzymes are proteins. Some enzymes require no chemical groups other than their amino acid residues for activity. Others require an additional chemical component called a cofactor, which may be either one or more inorganic ions, such as Fe 2+ , Mg 2+ , Mn 2+ , or Zn 2+ , or a more complex organic or metalloorganic molecule called a coenzyme.
  • a cofactor may be either one or more inorganic ions, such as Fe 2+ , Mg 2+ , Mn 2+ , or Zn 2+ , or a more complex organic or metalloorganic molecule called a coenzyme.
  • the catalytic activity provided by enzymes allows for much lower limits of detection than would be obtained with common binding techniques.
  • the catalytic activity of enzymes depends upon the integrity of their native protein conformation. If an enzyme is denatured, dissociated into its subunits, or broken down into its component amino acids, its catalytic activity is destroyed. Enzyme-coupled receptors can also be used to modify the recognition mechanisms.
  • the new vector, PT-cAu-TNF avoids detection and clearance by the RES, and actively and specifically sequesters TNF within a solid tumor.
  • the altered biodistribution correlated to improvements.
  • a preferred embodiment includes the use of PEGylated-Au nanoparticles-PA drug systems to avoid detection and clearance by the RES.
  • Binding can be performed through covalent bonds taking advantage of reactive groups such as amine (—NH 2 ) or sulfide (—SH) that naturally are present or can be incorporated into the biomolecule structure.
  • Amines can react with carboxylic acid or ester moieties in high yield to form stable amide bonds.
  • Thiols can participate in maleimide coupling, yielding stable dialkylsulfides.
  • a solid support of interest in the present invention is the metal (preferably gold or silver) nanoparticles.
  • the majority of immobilization schemes involving metal surfaces, such as gold or silver, utilize a prior derivatization of the surface with alkylthiols, forming stable linkages.
  • Alkylthiols readily form self-assembled monolayers (SAM) onto silver surfaces in micromolar concentrations.
  • SAM self-assembled monolayers
  • the terminus of the alkylthiol chain can be used to bind biomolecules, or can be easily modified to do so.
  • the length of the alkylthiol chain has been found to be an important parameter, keeping the biomolecules away from the surface, with lengths of the alkyl group from 4 to 20 carbons being preferred.
  • alkylthiols have been used to block further access to the surface, allowing only covalent immobilization through the linker [Steel, A. B.; Herne, T. M.; Tarlov, M. J. Anal. Chem. 1998, 70, 4670-7; Herne, T. M.; Tarlov, M. J. J. Am. Chem. Soc. 1997, 119, 8916-20]
  • a cyclic dithiane-epiandrosterone disulfide linker has been developed for binding oligonucleotides to gold surfaces [R. Elghanian, J. J. Storhoff, R. C. Mucic, R. L. Letsinger and C. A. Mirkin, Selective colorimetric detection of polynucleotides based on the distance - dependent optical properties of gold nanoparticles. Science 277 (1997), pp. 1078-1081]. Li et al.
  • 6666-6670 have shown that the base composition of the oligonucleotide has a significant effect on colloid stability and on oligonucleotide surface coverage.
  • Otsuka et al. have used a heterobifunctional thiol-PEG (polyethylene glycol) derivative as a linker to stabilize gold PRPs [H. Otsuka, Y. Akiyama, Y. Nagasaki and K. Kataoka, Quantitative and reversible lectin - induced association of gold nanoparticles modified with ⁇ - lactosyl - ⁇ - mercapto - poly ( ethylene glycol ). J. Am. Chem. Soc. 123 (2001), pp.8226-8230].
  • Proteins are usually bound to PANs using non-covalent, passive absorption.
  • a mercapto-undecanoic acid linker/spacer molecule can be used to attach NeutrAvidin covalently to gold and silver segmented nanorods [I. D. Walton, S. M. Norton, A. Balasingham, L. He, D. F. Oviso, D. Gupta, P. A. Raju, M. J. Natan and R. G. Freeman, Particles for multiplexed analysis in solution: detection and identification of striped metallic particles using optical microscopy. Anal. Chem. 74 (2002), pp. 2240-2247].
  • the thiol groups bind to the metal surface, and the carboxyl functional groups on the particle surface are activated using EDC and s-NHS reagents and then cross-linked to the amino groups in NeutrAvidin.
  • the ability to fabricate core-shell particles where the core is metal and the shell is composed of latex, silica, polystyrene or other non-metal material provides a promising alternative approach to immobilizing biomolecules and engineering particle surfaces [T. K. Mandal, M. S. Fleming and D. R. Walt, Preparation of polymer coated gold nanoparticles by surface - confined living radical polymerization at ambient temperature. Nano Letters 2 (2002), pp. 3-7; S. O. Obare, N. R.
  • Silver surfaces have been found to exhibit controlled self-assembly kinetics when exposed to dilute ethanolic solutions of alkylthiols.
  • the tilt angle formed between the surface and the hydrocarbon tail ranges from 0 to 15°.
  • SAM self-assembled monolayer
  • the coupling approach involves the esterification under mild conditions of a carboxylic acid with a labile group, an N-hydroxysuccinimide (NHS) derivative, and further reaction with free amine groups in a polypeptide (enzymes, antibodies, antigens, etc) or amine-labeled DNA, producing a stable amide [Boncheva, M.; Scheibler, L.; Lincoln, P.; Vogel, H.; Akerman, B. Langmuir 1999, 15, 4317-20].
  • NHS reacts almost exclusively with primary amine groups. Covalent immobilization can be achieved in as little as 30 minutes.
  • Maleimide can be used to immobilize biomolecules through available —SH moieties. Coupling schemes with maleimide have been proven useful for the site-specific immobilization of antibodies, Fab fragments, peptides, and SH-modified DNA strands.
  • Sample preparation for the maleimide coupling of a protein involves the simple reduction of disulfide bonds between two cysteine residues with a mild reducing agent, such as dithiothreitol, 2-mercaptoethanol or tris(2-carboxyethyl)phosphine hydrochloride. However, disulfide reduction will usually lead to the protein losing its natural conformation, and might impair enzymatic activity or antibody recognition.
  • Surfaces modified with mercaptoalkyldiols can be activated with 1,1′-carbonyldiimidazole (CDI) to form a carbonylimidazole intermediate.
  • CDI 1,1′-carbonyldiimidazole
  • a biomolecule with an available amine group displaces the imidazole to form a carbamate linkage to the alkylthiol tethered to the surface [Potyrailo, R. A., et al., 1998].
  • nanoparticles of metal colloid hydrosols are prepared by rapidly mixing a solution of AgNO 3 with ice-cold NaBH 4 .
  • a DNA segment is bound to a nanoparticle of silver or gold.
  • the immobilization of biomolecules usually takes advantage of reactive groups such as amine (—NH 2 ) or sulfide (—SH) that naturally are present or can be incorporated into the biomolecule structure.
  • Amines can react with carboxylic acid or ester moieties in high yield to form stable amide bonds.
  • Thiols can participate in maleimide coupling yielding stable dialkylsulfides.
  • silver nanoparticles are used.
  • the immobilization schemes involving Ag surfaces utilize a prior derivatization of the surface with alkylthiols, forming stable linkages are used.
  • Alkylthiols readily form self-assembled monolayers (SAM) onto silver surfaces in micromolar concentrations.
  • SAM self-assembled monolayers
  • the terminus of the alkylthiol chain can be directly used to bind biomolecules, or can be easily modified to do so.
  • the length of the alkylthiol chain was found to be an important parameter, keeping the biomolecules away from the surface.
  • alkylthiols were used to block further access to the surface, allowing only covalent immobilization through the linker.
  • Silver/gold surfaces have been found to exhibit controlled self-assembly kinetics when exposed to dilute ethanolic solutions of alkylthiols.
  • the tilt angle formed between the surface and the hydrocarbon tail ranges from 0 to 15°.
  • alkylthiols can be covalently coupled to biomolecules.
  • the majority of synthetic techniques for the covalent immobilization of biomolecules utilize free amine groups of a polypeptide (enzymes, antibodies, antigens, etc) or of amino-labeled DNA strands, to react with a carboxylic acid moiety forming amide bonds.
  • more active intermediate labile ester
  • Successful coupling procedures include:
  • the coupling approach used to bind DNA to a silver nanoparticle involves the esterification under mild conditions of a carboxylic acid with a labile group, an N-hydroxysuccinimide (NHS) derivative, and further reaction with free amine groups in a polypeptide (enzymes, antibodies, antigens, etc) or amine-labeled DNA, producing a stable amide [4].
  • NHS reacts almost exclusively with primary amine groups. Covalent immobilization can be achieved in as little as 30 minutes. Since H 2 O competes with —NH 2 in reactions involving these very labile esters, it is important to consider the hydrolysis kinetics of the available esters used in this type of coupling.
  • O—(N-succinimidyl)-N,N,N′,N′-tetramethyluronium tetrafluoroborate increases the coupling yield by utilizing a leaving group that is converted to urea during the carboxylic acid activation, hence favorably increasing the negative enthalpy of the reaction.
  • a plasmonics enhanced effect can occur throughout the electromagnetic region provided the suitable nanostructures, nanoscale dimensions, metal types are used. Therefore, the PEPST concept is valid for the entire electromagnetic spectrum, i.e, energy, ranging from gamma rays and X rays throughout ultraviolet, visible, infrared, microwave and radio frequency energy.
  • visible and NIR light are used for silver and gold nanoparticles, since the plasmon resonances for silver and gold occur in the visible and NIR region, respectively.
  • the NIR region is very appropriate for non-invasive therapy.
  • FIG. 13 shows a diagram of the therapeutic window of tissue. The following section discusses the use of one-photon and multi-photon techniques for therapy.
  • Two methods can be used, one-photon or multi-photon excitation. If the two-photon technique is used, one can excite the PA molecules with light at 700-1000 nm, which can penetrate deep inside tissue, in order to excite molecules that absorb in the 350-500 nm spectral region. This approach can excite the psoralen compounds, which absorb in the 290-350 nm spectral region and emit in the visible. With the one-photon method, the photo-activator (PA) drug molecules can directly absorb excitation light at 600-1300 nm. In this case we can design a psoralen-related system (e.g., psoralens having additional aromatic rings or other conjugation to alter the ability to absorb at different wavelengths) or use other PA systems: photodynamic therapy drugs, ALA, etc.
  • PA photo-activator
  • the present invention provides a solution to that problem, by the providing of a molecular system that can absorb the X-ray energy and change that energy into other energies that can be used to activate drug molecules. More specifically, the molecular system that can absorb and change the X-ray energy in the present invention is the PEPST probes comprising nanoparticles.
  • the present invention uses X-rays for excitation.
  • the advantage is the ability to excite molecules non-invasively since X-ray can penetrate deep in tissue.
  • the limitation is the fact that X-ray does not interact with most molecules.
  • the drug molecule or PA
  • the drug molecule is bound to a molecular entity, referred to as an “energy modulation agent” that can interact with the X-rays, and then emit light that can be absorbed by the PA drug molecules.
  • gold nanoparticles as plasmonics-active systems have been discussed. Furthermore, gold nanoparticles are also good energy modulation agent systems since they are biocompatible and have been shown to be a possible candidate for contrast agents for X-ray [Hainfeld et al, The British Journal of radiology, 79, 248, 2006].
  • the use of gold nanoparticles as a dose enhancer seems more promising than the earlier attempts using microspheres and other materials for two primary reasons.
  • the gold nanoparticles were non-toxic to mice aid were largely cleared from the body through the kidneys. This novel use of small gold nanoparticles permitted achievement of the high metal content in tumors necessary for significant high-Z radioenhancement [James F Hainfeld, Daniel N Slatkin and Henry M Smilowitz, The use of gold nanoparticles to enhance radiotherapy in mice, Phys. Med. Biol. 49 (2004)]
  • Z atomic number
  • nanoparticles provide a better mechanism than microspheres, in terms of delivering high-Z materials to the tumor, overcoming some of the difficulties found during an earlier attempt using gold microspheres [Sang Hyun Cho, Estimation of tumor dose enhancement due to gold nanoparticles during typical radiation treatments: a preliminary Monte Carlo study, Phys. Med. Biol. 50 (2005)]
  • Gold (or metal) complexes with PA can preferably be used in the present invention.
  • the metal can be used as an energy modulation agent system.
  • gold complexes with psoralen-related ligands can be used as a hybrid energy modulation agent-PA system.
  • the gold molecules serve as the energy modulation agent system and the ligand molecules serve as the PA drug system.
  • FIG. 15 shows a number of the various embodiments of PEPST probes that can be preferably used for X ray excitation of energy modulation agent-PA system. These probes comprise:
  • the following discussion is centered on gold as the metal material and CdS as the energy modulation agent material (which can also be used as DNA stabilized CdS, see Ma et al, Langmuir, 23 (26), 12783-12787 (2007)) and psoralen as the PA molecule.
  • metal material gold
  • CdS energy modulation agent material
  • psoralen psoralen
  • Suitable metals that can be used in plasmon resonating shells or other plasmon resonating structures can be include, but are not limited to, gold, silver, platinum, palladium, nickel, ruthenium, rhenium, copper, and cobalt.
  • the PEPST system comprises gold nanoparticles, an energy modulation agent nanoparticle (e.g., CdS) linked to a PA drug molecule (e.g., psoralen).
  • CdS energy modulation agent nanoparticle
  • PA drug molecule e.g., psoralen
  • X ray is irradiated to CdS, which absorbs X rays [Hua et al, Rev. Sci. Instrum., 73, 1379, 2002] and emits CdS XEOL light (at 350-400 nm) that is plasmonics-enhanced by the gold nanoparticle.
  • This enhanced XEOL light is used to photoactivate psoralen (PA molecule).
  • PA molecule photoactivate psoralen
  • the nanostructure of the gold nanoparticle is designed to enhance the XEOL light at 350-400 nm.
  • the PEPST system comprises a plasmonics-active metal (gold) nanoparticle with energy modulation agent nanocap (CdS) covered with PA molecules (e.g., psoralen).
  • Au plasmonics-active metal
  • PA molecules e.g., psoralen.
  • X ray is irradiated to CdS, which absorbs X ray and emits XEOL light that is plasmonics-enhanced by the gold nanoparticle. This enhanced XEOL light is used to photoactivate psoralen (PA molecule).
  • the PEPST system comprises a PA (e.g., psoralen)-covered CdS nanoparticle with smaller plasmonic metal (gold) nanoparticles.
  • PA e.g., psoralen
  • Au plasmonic metal
  • the energy modulation agent core comprises CdS or CsCl nanoparticles covered with a nanocap of gold.
  • X ray is irradiated to CdS or CsCl, which absorbs X ray [[Jaegle et al, J. Appl. Phys., 81, 2406, 1997] and emits XEOL light that is plasmonics-enhanced by the gold nanocap structure. This enhanced XEOL light is used to photoactivate psoralen (PA molecule).
  • the embodiment in FIG. 15E comprises a spherical gold core covered by a shell of CdS or CsCl.
  • X ray is irradiated to CdS or CsCl material, which absorbs X ray [Jaegle et al., J. Appl. Phys., 81, 2406, 1997] and emits XEOL light that is plasmonics-enhanced by the gold nanosphere.
  • This enhanced XEOL light is used to photoactivate psoralen (PA molecule).
  • the PEPST system comprises gold nanoparticles, and an energy modulation agent nanoparticle (e.g., CdS) linked to a PA drug molecule (e.g., psoralen) by a link that can be detached by radiation.
  • CdS energy modulation agent nanoparticle
  • PA drug molecule e.g., psoralen
  • X ray is irradiated to CdS, which absorbs X ray and emits CdS XEOL light (at 350-400 nm) that is plasmonics-enhanced by the gold nanoparticle.
  • This enhanced XEOL light is used to photoactivate psoralen (PA molecule).
  • PA molecule photoactivate psoralen
  • the nanostructure of the gold nanoparticle is designed to enhance the XEOL light at 350-400 nm.
  • the metal nanoparticles or single nanoshells are replaced by multi layers of nanoshells [Kun Chen, Yang Liu, Guillermo Ameer, Vadim Backman, Optimal design of structured nanospheres for ultrasharp light - scattering resonances as molecular imaging multilabels, Journal of Biomedical Optics, 10(2), 024005 (March/April 2005)].
  • the metal nanoparticles are covered with a layer (1-30 nm) of dielectric material (e.g. silica).
  • the dielectric layer (or nanoshell) is designed to prevent quenching of the luminescence light emitted by the energy modulation agent (also referred to as EEC) molecule(s) due to direct contact of the metal with the energy modulation agent molecules.
  • the energy modulation agent molecules or materials are bound to (or in proximity of) a metal nanoparticle via a spacer (linker). The spacer is designed to prevent quenching of the luminescence light emitted by the energy modulation agent molecules or materials.
  • the energy modulation agent materials can include any materials that can absorb X ray and emit light in order to excite the PA molecule.
  • the energy modulation agent materials include, but are not limited to:
  • organic solids metal complexes, inorganic solids, crystals, rare earth materials (lanthanides), polymers, scintillators, phosphor materials, etc.;
  • Quantum dots, semiconductor nanostructures Various materials related to quantum dots, semiconductor materials, etc. can be used as energy modulation agent systems. For example CdS-related nanostructures have been shown to exhibit X-ray excited luminescence in the UV-visible region [Hua et al, Rev. Sci. Instrum., 73, 1379, 2002].
  • Scintillator Materials as energy modulation agent systems.
  • Various scintillator materials can be used as energy modulation agents since they absorb X-ray and emit luminescence emission, which can be used to excite the PA system.
  • single crystals of molybdates can be excited by X-ray and emit luminescence around 400 nm [Mirkhin et al, Nuclear Instrum. Meth. In Physics Res. A, 486, 295 (2002].
  • Solid Materials as energy modulation agent systems Various solid materials can be used as energy modulation agents due to their X-ray excited luminescence properties. For example CdS (or CsCl) exhibit luminescence when excited by soft X-ray [Jaegle et al, J. Appl. Phys., 81, 2406, 1997].
  • XEOL materials lanthanides or rare earth materials [L. Soderholm, G. K Liu, Mark R. Antonioc, F. W Lytle, X - ray excited optical luminescence .XEOL. detection of x - ray absorption fine structure .XAFZ, J. Chem. Phys, 109, 6745, 1998], Masashi Ishiia, Yoshihito Tanaka and Tetsuya Ishikawa, Shuji Komuro and Takitaro Morikawa, Yoshinobu Aoyagi, Site - selective x - ray absorption fine structure analysis of an optically active center in Er - doped semiconductor thin film using x - ray - excited optical luminescence, Appl. Phys. Lett, 78, 183, 2001]
  • FIGS. 16 and 17 Some examples of metal complexes exhibiting XEOL which can be used as energy modulation agent systems are shown in FIGS. 16 and 17 . Such structures can be modified by replacing the metal atom with metal nanoparticles in order to fabricate a plasmonics-enhance PEPST probe.
  • the experimental parameters including size, shape and metal type of the nano structure can be selected based upon the excitation radiation (NIR or X ray excitation), the photoactivation radiation (UVB), and/or the emission process from the energy modulation agent system (visible NIR).
  • U.S. Pat. No. 7,008,559 (the entire contents of which are incorporated herein by reference) describes the upconversion performance of ZnS where excitation at 767 nm produces emission in the visible range.
  • the materials described in U.S. Pat. No. 7,008,559 (including the ZnS as well as Er 3+ doped BaTiO 3 nanoparticles and Yb 3+ doped CsMnCl 3 ) are suitable in various embodiments of the invention.
  • Such materials may be any semiconductor and more specifically, but not by way of limitation, sulfide, telluride, selenide, and oxide semiconductors and their nanoparticles, such as Zn 1 ⁇ x Mn x S y , Zn 1 ⁇ x Mn x Se y , Zn 1 ⁇ x Mn x Te y , Cd 1 ⁇ x MnS y , Cd 1 ⁇ x Mn x Se y , Cd 1 ⁇ x Mn x Te y , Pb 1 ⁇ x Mn x S y , Pb 1 ⁇ x Mn x Se y , Pb 1 ⁇ x Mn x Te y , Mg 1 ⁇ x MnS y , Ca 1 ⁇ x Mn x S y , Ba 1 ⁇ x Mn
  • Additional energy modulation materials include insulating and nonconducting materials such as BaF 2 , BaFBr, and BaTiO 3 , to name but a few exemplary compounds.
  • nanoparticles such as ZnS:Tb 3+ , Er 3 ⁇ ; ZnS:Tb 3+ ; Y 2 O 3 :Tb 3+ ; Y 2 O 3 :Tb 3+ , Er 3+ ; ZnS:Mn 2+ ; ZnS:Mn,Er 3+ are known in the art to function for both down-conversion luminescence and upconversion luminescence, and can thus be used in various embodiments of the present invention.
  • One embodiment of the basic PEPST probe embodiment comprises PA molecules bound to an energy modulation agent and to plasmonic metal (gold) nanoparticles.
  • the metal nanoparticle can serve as a drug delivery platform (see previous discussion).
  • the metal nanoparticle can play 2 roles:
  • the X ray radiation, used to excite the energy modulation agent system, is amplified by the metal nanoparticle due to plasmon resonance.
  • the energy modulation agent system exhibits more emission light that is used to photoactivate the PA drug molecules (e.g., psoralens) and make them photoactive.
  • the metal nanoparticles are designed to exhibit strong plasmon resonance at or near the X ray wavelengths.
  • the surface plasmon resonance effect amplifies the excitation light at the nanoparticles, resulting in increased photoactivation of the PA drug molecules and improved therapy efficiency.
  • the plasmonics-enhanced mechanism can also be used with the other PEPST probes described above.
  • FIG. 18 illustrates the plasmonics-enhancement effect of the PEPST probe.
  • Soft X ray can go to 10 nm.
  • the dimension of plasmonics-active nanoparticles usually have dimensions on the order or less than the wavelengths of the radiation used. Note that the approximate atomic radius of gold is approximately 0.15 nanometers. At the limit, for gold the smallest “nanoparticle” size is 0.14 nm (only 1 gold atom). A nanoparticle with size in the hundreds of nm will have approximately 10 6 -10 7 gold atoms. Therefore, the range of gold nanoparticles discussed in this invention can range from 1-10 7 gold atoms.
  • the gold nanoparticles can also enhance the energy modulation agent emission signal, which is use to excite the PA molecule.
  • this spectral range is in the UVB region (320-400nm).
  • Silver or gold nanoparticles, nanoshell and nanocaps have been fabricated to exhibit strong plasmon resonance in this region.
  • FIG. 19 shows excitation and emission fluorescence spectra of a psoralen compound (8-methoxypsoralen).
  • PEPST Energy Modulation Agent-PA Probe with Detachable PA PEPST Energy Modulation Agent-PA Probe with Detachable PA.
  • FIG. 20 shows an embodiment of a PEPST probe where the PA drug molecule is bound to the metal nanoparticles via a linker ( FIG. 20A ) that can be cut by photon radiation ( FIG. 20B ).
  • a linker FIG. 20A
  • FIG. 20B Such a probe is useful for therapy modalities where the PA molecules have to enter the nucleus, e.g., psoralen molecules need to enter the nucleus of cells and intercalate onto DNA ( FIG. 20C ). Since it is more difficult for metal nanoparticles to enter the cell nucleus than for smaller molecules, it is preferable to use PEPST probes that have releasable PA molecules.
  • Suitable linkers for linking the PA drug molecule to the metal nanoparticles include, but are not limited to, labile chemical bonds that can be broken by remote energy excitation (from outside the body, e.g., MW, IR, photoacoustic energy, ultrasound energy, etc.), labile chemical bonds that can be broken by the chemical environment inside cells, antibody-antigen, nucleic acid linkers, biotin-streptavidin, etc.
  • FIG. 21 illustrates an embodiment of the present invention PEPST probe having a chain of metal particles having different sizes and coupled to each other, which could exhibit such dual plasmonics-based enhancement.
  • the parameters (size, metal type, structure, etc) of the larger nanoparticle can be tuned to NIR, VIS or UV light while the smaller particle ( FIG. 21 , right) can be tuned to X ray. There is also a coupling effect between these particles.
  • nanoparticle chains are useful in providing plasmonics enhancement of both the incident radiation used (for example, x-ray activation of CdS) as well as plasmonics enhancement of the emitted radiation that will then activate the PA.
  • Similar nanoparticles systems have been used as nanolens [ Self - Similar Chain of Metal Nanospheres as an Efficient Nanolens, Kuiru Li, Mark L Stockman, and David J. Bergman, Physical Review Letter, VOLUME 91, NUMBER 22, 227402-1, 2003].
  • the field of particle-based drug delivery is currently focused on two chemically distinct colloidal particles, liposomes and biodegradable polymers. Both delivery systems encapsulate the active drug. The drug is released from the particle as it lyses, in the case of lipsomes, or disintegrates, as described for biodegradable polymers.
  • One embodiment of the present invention uses liposomal delivery of energy modulation agent-PA systems (e.g., gold nanoshells) for therapy.
  • energy modulation agent-PA systems e.g., gold nanoshells
  • the liposome preparation method is adapted from Hölig et.al Hölig, P., Bach, M., Völkel, T., Nahde, T., Hoffmann, S., Müller, R., and Kontermann, R. E., Novel RGD lipopeptides for the targeting of liposomes to integrin - expressing endothelial and melanoma cells. Protein Engineering Design and Selection, 2004. 17(5): p. 433-441]. Briefly, the lipids PEG-DPPE, PC, and Rh-DPPE are mixed in chloroform in a round bottom flask and evaporated (Hieroglyph Rotary Evaporator, Rose Scientific Ltd., Edmonton, Alberta, Canada) to eliminate chloroform.
  • the dry film is dehydrated into aqueous phase with using PBS solution.
  • a dry lipid film is prepared by rotary evaporation from a mixture of PC, cholesterol, and PEG-DPPE and then hydrated into aqueous phase using PBS.
  • the mixture is vigorously mixed by overtaxing and bath solicited (Instrument, Company) and the suspension extruded through polycarbonate filter using Liposofast apparatus (Avestin Inc., Ottawa, ON, Canada) (pore-size 0.8 ⁇ m).
  • Preparation of liposomes is performed as follows; 0.1 mmol of PC is dispersed in 8 ml of chloroform and supplemented with 0.5 mol of PEG-DPPE in 20 ml of chloroform.
  • rhodamine-labeled phosphatidylethanolamine Rh-DPPE
  • the organic solvents are then removed by rotary evaporation at 35° C. for 2 h leaving a dry lipid film.
  • Gold nanoshells are encapsulated into liposomes by adding them to the PBS hydration buffer and successively into the dry lipid film. This mixture is emulsified in a temperature controlled sonicator f)r 30 minutes at 35° C. followed by vortexing for 5 min. Encapsulated gold nanoshells are separated from unencapsulated gold nanoshells by gentle centrifugation for 5 minutes at 2400 r.p.m (1200 g).
  • the resulting multilamellar vesicles suspension is extruded through polycarbonate filter using Liposofast apparatus (Avestin Inc., Ottawa, ON, Canada) (pore-size 0.8 ⁇ m).
  • the aqueous mixture is obtained and stored at 4° C.
  • the color of the solution gradually changed from the initial faint yellowish to clear, grey, purple and finally a tantalizing wine-red color similar to merlot.
  • the sodium citrate used serves in a dual capacity, first acting as a reducing agent, and second, producing negative citrate ions that are adsorbed onto the gold nanoparticles introducing surface charge that repels the particles and preventing nanocluster formation.
  • Liposome-encapsulated gold nanoshells are incubated with MCF-7 cells grown on partitioned cover-slips for intracellular delivery. This is done by adding 10 ⁇ l of liposome-encapsulated gold nanoshells per 1 ml of cell culture medium. This is incubated for 30 minutes in a humidified (86% RH) incubator at 37° C. and 5% CO 2 . This cell is used for localization studies; to track the rhodamine-DPPE-labeled liposomes into the cytoplasm of the MCF-7 cell. After incubation, the cells grown on cover-slips are washed three times in cold PBS and fixed using 3.7% formaldehyde in PBS. Rhodamine staining by rhodamine-DPPE-labeled liposomes is analyzed using a Nikon Diaphot 300 inverted microscope (Nikon, Inc., Melville, N.Y.).
  • the PA system e.g. psoralen
  • the PA is still linked to the energy modulation agent, both of them have to be transported into the nucleus.
  • gold nanoparticles as the energy modulation agent system
  • An example of linkage is through a chemical bond or through a bioreceptor, such as an antibody.
  • the PA is the antigen molecule bound to the energy modulation agent system that has an antibody targeted to the PA.
  • the PA molecules can be released from the energy modulation agent Ab system.
  • chemical reagents can be used to cleave the binding between antibody and antigen, thus regenerating the biosensor [Vo-Dinh et al, 1988].
  • This chemical procedure is simple but is not practical inside a cell due to possible denaturation of the cell by the chemical.
  • the gentle but effective MHz-range ultrasound has the capability to release antigen molecules from the antibody-energy modulation agent system [Moreno-Bondi, M., Mobley, J., and Vo-Dinh, T., “Regenerable Antibody-based Biosensor for Breast Cancer,” J. Biomedical Optics, 5, 350-354 (2000)].
  • an alternative embodiment is to use gentle ultrasonic radiation (non-invasively) to remove the PA (antigen) from the antibody at the energy modulation agent system.
  • the PA molecule is bound to the energy modulation agent by a chemically labile bond
  • a chemically labile bond [Jon A. Wolff, and David B. Rozema, Breaking the Bonds: Non-viral Vectors Become Chemically Dynamic, Molecular Therapy ( 2007) 16(1), 8-15].
  • a promising method of improving the efficacy of this approach is to create synthetic vehicles (SVs) that are chemically dynamic, so that delivery is enabled by the cleavage of chemical bonds upon exposure to various physiological environments or external stimuli.
  • An example of this approach is the use of masked endosomolytic agents (MEAs) that improve the release of nucleic acids from endosomes, a key step during transport. When the MEA enters the acidic environment of the endosome, a pH-labile bond is broken, releasing the agent's endosomolytic capability.
  • MEAs masked endosomolytic agents
  • Another embodiment to deliver the energy modulation agent-PA drugs involves the use of ferritin and apoferritin compounds.
  • ferritin and apoferritin compounds There is increasing interest in ligand-receptor-mediated delivery systems due to their non-immunogenic and site-specific targeting potential to the ligand-specific bio-sites.
  • Platinum anticancer drug have been encapsulated in apoferritin [Zhen Yang, Xiaoyong Wang, Huajia Diao, Junfeng Zhang, Hongyan Li, Hongzhe Sun and Zijian Guo, Encapsulation of platinum anticancer drugs by apoferritin, Chem. Commun. 33, 2007, 3453-3455].
  • Ferritin the principal iron storage molecule in a wide variety of organisms, can also be used as a vehicle for targeted drug delivery.
  • apoferritin contains a hollow protein shell, apoferritin, which can contain up to its own weight of hydrous ferric oxide-phosphate as a microcrystalline micelle.
  • the 24 subunits of ferritin assemble automatically to form a hollow protein cage with internal and external diameters of 8 and 12 nm, respectively.
  • Gd 3+ gadolinium contrast agents
  • desferrioxamire B desferrioxamire B
  • metal ions metal ions
  • nanoparticles of iron salts can be accommodated in the cage of apoferritin.
  • Zinc selenide nanoparticles were synthesized in the cavity of the cage-shaped protein apoferritin by designing a slow chemical reaction system, which employs tetraaminezinc ion and selenourea.
  • the chemical synthesis of ZnSe NPs was realized in a spatially selective manner from an aqueous solution, and ZnSe cores were formed in almost all apoferritin cavities with little bulk precipitation [Kenji Iwahori, Keiko Yoshizawa, Masahiro Muraoka, and Ichiro Yamashita, Fabrication of ZnSe Nanoparticles in the Apoferritin Cavity by Designing a Slow Chemical Reaction System, Inorg. Chem., 44 (18), 6393-6400, 2005].
  • Apoferritin has a cavity, 7 nm in diameter, and the diameter of fabricated Au 2 S nanoparticles is about the same size with the cavity and size dispersion was small.
  • the PA or energy modulation agent-PA compounds are encapsulated inside the apoferrtin shells.
  • ferritin could be internalized by some tumor tissues, and the internalization was associated with the membrane-specific receptors [S. Fargion, P. Arosio, A. L. Fracanzoni, V. Cislaghi, S. Levi, A. Cozzi, A Piperno and A. G. Firelli, Blood, 1988, 71, 753-757; P. C. Adams, L. W. Powell and J. W. Halliday, Hepatology, 1988, 8, 719-721].
  • Previous studies have shown that ferritin-binding sites and the endocytosis of ferritin have been identified in neoplastic cells [M. S. Bretscher and J. N. Thomson, EMBO J., 1983, 2, 599-603].
  • Ferritin receptors have the potential for use in the delivery of anticancer drugs into the brain [S. W. Hulet, S. Powers and J. R. Connor, J. Neurol. Sci., 1999, 165, 48-55].
  • the present invention uses ferritin or apoferritin to both encapsulate PA and energy modulation agent-PA systems and also target tumor cells selectively for enhanced drug delivery and subsequent phototherapy. In this case no additional bioreactors are needed.
  • FIG. 22 schematically illustrates the use of encapsulated photoactive agents ( FIG. 22A ) for delivery into tissue and subsequent release of the photoactive drugs after the encapsulated systems enter the cell.
  • the encapsulated system can have a bioreceptor for selective tumor targeting ( FIG. 22B ).
  • the capsule shell e.g., liposomes, apoferritin, etc.
  • non-invasive excitation e.g., ultrasound, RF, microwave, IR, etc
  • FIG. 23 illustrates the basic operating principle of the PEPST modality.
  • the PEPST photoactive drug molecules are given to a patient by oral ingestion, skin application, or by intravenous injection.
  • the PEPST drugs travel through the blood stream inside the body towards the targeted tumor (either via passive or active targeting strategies).
  • a photon radiation at suitable wavelengths is used to irradiate the skin of the patient, the light being selected to penetrate deep inside tissue (e.g., NIR or X ray).
  • the radiation light source is directed at the tumor.
  • a treatment procedure can be initiated using delivery of energy into the tumor site.
  • One or several light sources may be used as described in the previous sections.
  • One embodiment of therapy comprises sending NIR radiation using an NIR laser through focusing optics. Focused beams of other radiation types, including but not limited to X ray, microwave, radio waves, etc. can also be used and will depend upon the treatment modalities used.
  • Excitons are often defined as “quasiparticles” inside a solid material.
  • solid materials such as semiconductors, molecular crystals and conjugated organic materials
  • light excitation at suitable wavelength such as X ray, UV and visible radiation, etc
  • suitable wavelength such as X ray, UV and visible radiation, etc
  • this neutral bound complex is a “quasiparticle” that can behave as a boson—a particle with integer spin which obeys Bose-Einstein statistics: when the temperature of a boson gas drops below a certain value, a large number of bosons ‘condense’ into a single quantum state—this is a Bose-Einstein condensate (BEC).
  • BEC Bose-Einstein condensate
  • Exciton production is involved in X-ray excitation of a solid material. Wide band-gap materials are often employed for transformation of the x-ray to ultraviolet/visible photons in the fabrication of scintillators and phosphors [Martin Nikl, Scintillation detectors for x - rays, Meas. Sci. Technol.
  • the final stage, luminescence consists in consecutive trapping of the electron-hole pairs at the luminescent centers and their radiative recombination.
  • the electron-hole pairs can be trapped at the defects and recombine, producing luminescent.
  • Luminescent dopants can also be used as traps for exciton.
  • Exciton traps can be produced using impurities in the crystal host matrix.
  • the electron trap states may arise when electron is localized on a neighbor of the impurity molecule.
  • Such traps have been observed in anthracene doped with carbazole [Kadshchuk, A. K., Ostapenko, N. I., Skryshevskii, Yu. A., Sugakov, V. I. and Susokolova, T. O., Mol. Cryst. and Liq. Cryst., 201, 167 (1991)].
  • the formation of these traps is due to the interaction of the dipole moment of the impurity with charge carrier.
  • FIG. 25 shows various embodiments of E-[P probes that can be designed:
  • the embodiment in probes B provide the capability to tune the energy conversion from an X ray excitation source into a wavelength of interest to excite the PA molecules.
  • PHA polynuclear aromatic hydrocarbons
  • Tunable EIP probes can be designed to contain such luminescent dopants such as highly luminescent PAHs exhibiting luminescence emission in the range of 300-400 nm suitable to activate psoralen.
  • a preferred embodiment of the EIP with tunable emission comprises a solid matrix (semiconductors, glass, quartz, conjugated polymers, etc) doped with naphthalene, phenanthrene, pyrene or other compounds exhibiting luminescence (fluorescence) in the 300-400 nm range [T Vo-Dinh, Multicomponent analysis by synchronous luminescence spectrometry, Anal. Chem.; 1978; 50(3) pp 396-401]. See FIG. 26 .
  • EEC matrix could be a semiconductor material, preferably transparent at optical wavelength of interest (excitation and emission).
  • FIG. 27 shows the X ray excitation optical luminescence (XEOL) of Europium doped in a matrix of BaFBr, emitting at 370-420 nm.
  • XEOL X ray excitation optical luminescence
  • FIG. 28 shows various embodiments of EIP probes that can be designed:
  • (A) probe comprising PA molecules bound around the energy modulation agent particle or embedded in a shell around an energy modulation agent particle that can produce excitons under radiative excitation at a suitable wavelength (e.g., X-ray).
  • a suitable wavelength e.g., X-ray
  • the energy modulation agent materials has structural defects that serve as traps for excitons.
  • (B) probe comprising PA molecules bound around the energy modulation agent particle or embedded in a shell around an energy modulation agent particle that can produce excitons under radiative excitation at a suitable wavelength (e.g., X-ray).
  • the energy modulation agent materials have impurities or dopant molecules that serve as traps for excitons.
  • a fundamental key concept in photophysics is the formation of new quasiparticles from admixtures of strongly-coupled states. Such mixed states can have unusual properties possessed by neither original particle.
  • the coupling between excitons and plasmons can be either weak or strong. When the light-matter interaction cannot be considered as a perturbation, the system is in the strong coupling regime. Bellesa et al showed a strong coupling between a surface plasmon (SP) mode and organic excitons occurs; the organic semiconductor used is a concentrated cyanine dye in a polymer matrix deposited on a silver film [Ref: J. Bellessa, * C. Bonnand, and J. C. Plenet, J.
  • Bondarev et al also described a theory for the interactions between excitonic states and surface electromagnetic modes in small-diameter ( ⁇ 1 nm) semiconducting single-walled carbon nanotubes (CNs). [I. V Bondarev, K Tatur and L. M. Woods, Strong exciton - plasmon coupling in semiconducting carbon nanotubes].
  • the composite metal-insulator-semiconductor nanowires ((Ag)SiO 2 )CdSe act as a waveguide for 1D-surface plasmons at optical frequencies with efficient photon out coupling at the nanowire tips, which is promising for efficient exciton-plasmon-photon conversion and surface plasmon guiding on a submicron scale in the visible spectral range.
  • Experiments on colloidal solutions of Ag nanoparticles covered with J-aggregates demonstrated the possibility of using the strong scattering cross section and the enhanced field associated with surface plasmon to generate stimulated emission from J-aggregate excitons with very low excitation powers. [Gregory A. Wurtz, * Paul R.
  • FIG. 29 shows various embodiments of EPEP probes of the present invention showing the exciton-plasmon coupling:
  • FIG. 30 shows yet further embodiments of EPEP probes of the present invention:
  • (A) probe comprising a PA molecule or group of PA molecules bound (through a linker, which can be fixed or detachable) to an energy modulation agent particle that can produce excitons under radiative excitation at a suitable wavelength (e.g., X-ray).
  • the energy modulation agent particle is covered with a nanoshell of silica (or other dielectric material), which is covered by a layer of separate nanostructures (nano islands, nanorods, nanocubes, etc. . . . ) of metal (Au, Ag).
  • the silica layer (or other dielectric material) is designed to prevent quenching of the luminescence light emitted by the EEC (also referred to as energy modulation agent) particle excited by X-ray.
  • the metal nanostructures are designed to induce plasmons that enhance the X ray excitation that subsequently leads to an increase in the EEC light emission, ultimately enhancing the efficiency of photoactivation, i.e. phototherapy.
  • the structure of the nanoparticle can also be designed such that the plasmonics effect also enhance the energy modulation agent emission light. These processes are due to strong coupling between excitons (in the energy modulation agent materials and plasmons in the metal nanostructures).
  • (B) probe comprising a group of PA molecules in a particle bound (through a linker, which can be fixed or detachable) to an energy modulation agent particle that can produce excitons under radiative excitation at a suitable wavelength (e.g., X-ray).
  • the PA-containing particle is covered with a layer of metallic nanostructures (Au, Ag).
  • the metal nanostructures (Au, Ag, etc) are designed to induce plasmons that enhance the energy modulation agent light emission, ultimately enhancing the efficiency of photoactivation, i.e. phototherapy.
  • (C) probe comprising a PA molecule or group of PA molecules bound (through a linker, which can be fixed or detachable) to an energy modulation agent particle that can produce excitons under radiative excitation at a suitable wavelength (e.g., X-ray).
  • the energy modulation agent particle is covered with a nanoshell of silica (or other dielectric material), which is covered by a layer of metallic nanostructures (Au, Ag).
  • the silica layer (or other dielectric material) is designed to prevent quenching of the luminescence light emitted by the energy modulation agent particle excited by X-ray.
  • the metal nanostructures are designed to induce plasmons that enhance the X ray excitation that subsequently leads to an increase in the energy modulation agent light emission, ultimately enhancing the efficiency of photoactivation, i.e. phototherapy.
  • the PA-containing particle is covered with a layer of metallic nanostructures (Au, Ag).
  • the metal nanostructures (Au, Ag, etc) are designed to induce plasmons that enhance the EEC light emission, ultimately enhancing the efficiency of photoactivation, i.e. phototherapy.
  • EPEP probes can also comprise hybrid self-assembled superstructures made of biological and abiotic nanoscale components, which can offer versatile molecular constructs with a spectrum of unique electronic, surface properties and photospectral properties for use in phototherapy.
  • Biopolymers and nanoparticles can be integrated in superstructures, which offer unique functionalities because the physical properties of inorganic nanomaterials and the chemical flexibility/specificity of polymers can be used. Noteworthy are complex systems combining two types of excitations common in nanomaterials, such as excitons and plasmons leading to coupled excitations.
  • Molecular constructs comprising building blocks including metal, semi conductor nanoparticles (NPs), nanorods (NRs) or nanowires (NWs) can produce EPEP probes with an assortment of photonic properties and enhancement interactions that are fundamentally important for the field of phototherapy.
  • Some examples of assemblies of some NW nanostructures and NPs have been reported in biosensing.
  • Nanoscale superstructures made from CdTe nanowires (NWs) and metal nanoparticles (NPs) are prepared via bioconjugation reactions.
  • Prototypical biomolecules such as D-biotin and streptavidin pair, were utilized to connect NPs and NWs in solution. It was found that Au NPs form a dense shell around a CdTe NW.
  • the superstructure demonstrated unusual optical effects related to the long-distance interaction of the semiconductor and noble metal nanocolloids.
  • the NWNP complex showed 5-fold enhancement of luminescence intensity and a blue shift of the emission peak as compared to unconjugated NW.
  • FIG. 31 shows various embodiments of EPEP probes of the present invention comprising superstructures of NPs, NWs and NRs.:
  • (A) probe comprising a PA molecule or group of PA molecules bound (through a linker, which can be fixed or detachable) to an energy modulation agent particle that can produce excitons under radiative excitation at a suitable wavelength (e.g., X-ray).
  • the energy modulation agent particle is bound to (or in proximity of) a metal nanowire (or nanorod) covered with a nanoshell cylinder of silica (or other dielectric material).
  • the silica nanoshells cylinder is designed to prevent quenching of the luminescence light emitted by the energy modulation agent particle excited by X-ray.
  • the metal nanoparticle (Au, Ag, etc) is designed to induce plasmons that enhance the X ray excitation that subsequently leads to an increase in the energy modulation agent light emission, ultimately enhancing the efficiency of photoactivation, i.e. phototherapy.
  • the structure of the nanoparticle can also be designed such that the plasmonics effect and/or the exciton-plasmon coupling (EPC) effect also enhances the energy modulation agent emission light. These processes are due to strong coupling between excitons (in the energy modulation agent materials and plasmons in the metal nanoparticles; and
  • (B) probe comprising a PA molecule or group of PA molecules bound (through a linker, which can be fixed or detachable) to an energy modulation agent particle that can produce excitons under radiative excitation at a suitable wavelength (e.g., X-ray).
  • the energy modulation agent particle is bound to (or in proximity of) a metal nanoparticles via a spacer (linker).
  • the spacer is designed to prevent quenching of the luminescence light emitted by the energy modulation agent particle excited by X-ray. Same effect as above in (A)
  • FIG. 32 shows another set of embodiments of EPEP probes of the present invention comprising superstructures of NPs, NWs and NRs and bioreceptors (antibodies, DNA, surface cell receptors, etc.).
  • bioreceptors antibodies, DNA, surface cell receptors, etc.
  • the use of bioreceptors to target tumor cells has been discussed previously above in relation to PEPST probes. Note that in this embodiment the PA molecules are attached along the NW axis in order to be excited by the emitting light form the NWs.
  • FIG. 33 shows another embodiment of EPEP probes of the present invention comprising superstructures of NPs linked to multiple NWs.
  • the metal nanostructures can be designed to amplify (due to the plasmonics effect) the excitation radiation (e.g., X-ray) and/or the emission radiation (e.g, UV or visible) to excite the photo-active (PA) molecule, thereby enhancing the PA effectiveness.
  • the excitation radiation e.g., X-ray
  • the emission radiation e.g, UV or visible
  • the energy modulation agent system can be designed to serve also as a microresonator having micron or submicron size.
  • Lipson et al described a resonant microcavity and, more particularly, to a resonant microcavity which produces a strong light-matter interaction [M. Lipson; L. C. Kimerling; Lionel C, Resonant microcavities, U.S. Pat. No. 6,627,923, 2000].
  • a resonant microcavity typically, is formed in a substrate, such as silicon, and has dimensions that are on the order of microns or fractions of microns.
  • the resonant microcavity contains optically-active matter (i.e., luminescent material) and reflectors which confine light in the optically-active matter.
  • the confined light interacts with the optically-active matter to produce a light-matter interaction.
  • the light-matter interaction in a microcavity can be characterized as strong or weak. Weak interactions do not alter energy levels in the matter, whereas strong interactions alter energy levels in the matter. In strong light-matter interaction arrangements, the confined light can be made to resonate with these energy level transitions to change properties of the microcavity.
  • Procedures for preparing gold and silver colloids include electroexplosion, electrodeposition, gas phase condensation, electrochemical methods, and solution-phase chemical methods.
  • the methodologies for preparing homogeneous-sized spherical colloidal gold populations 2-40 nm in diameter are well known [N. R. Jana, L. Gearheart and C. J. Murphy, Seeding growth for size control of 5-40 nm diameter gold nanoparticles. Langmuir 17(2001), pp. 6782-6786], and particles of this size are commercially available.
  • An effective chemical reduction method for preparing populations of silver particles (with homogeneous optical scattering properties) or gold particles (with improved control of size and shape monodispersity) is based on the use of small-diameter uniform-sized gold particles as nucleation centers for the further growth of silver or gold layers.
  • a widely used approach involves citrate reduction of a gold salt to produce 12-20 nm size gold particles with a relatively narrow size distribution.
  • the commonly used method for producing smaller gold particles was developed by House et al [Brust, M.; Walker, M.; Bethell, D.; Schiffrin, D. J.; Whyman, R. Chem. Commun. 1994, 801].
  • This method is based on borohydride reduction of gold salt in the presence of an alkanethiol capping agent to produce 1-3 nmparticles.
  • Nanoparticle sizes can be controlled between 2 and 5 nm by varying the thiol concentration, [Hostetler, M. J.; Wingate, J. E.; Zhong, C. J.; Harris, J.
  • Phosphine-stabilized gold clusters have also been produced and subsequently converted to thiol-capped clusters by ligand exchange in order to improve their stability [Schmid, G.; Pfeil, R.; Boese, R.; Bandrmann, F.; Meyer, S.; Calis, G. H. M; van der Velden, J. W. A. Chem. Ber.
  • metal nanoparticles coated with nanoshells of dye molecules can be performed using the method described by Masuhara et al [AKITO MASUHARA_, SATOSHI OHHASHIy, HITOSHI KASAI; SHUJI OKADA, FABRICATION AND OPTICAL PROPERTIES OF NANOCOMPLEXES COMPOSED OF METAL NANOPARTICLES AND ORGANIC DYES, Journal of Nonlinear Optical Physics & Materials Vol. 13, Nos. 3 & 4 (2004) 587-592].
  • Nanocomplexes composed of Ag or Au as a core and 3-carboxlymethyl-5-[2-(3-octadecyl-2-benzoselenazolinylidene) ethylidene]rhodanine (MCSe) or copper (II) phthalocyanine (CuPc) as a shell are prepared by the co-reprecipitation method.
  • MCSe 3-carboxlymethyl-5-[2-(3-octadecyl-2-benzoselenazolinylidene) ethylidene]rhodanine (MCSe) or copper (II) phthalocyanine (CuPc) as a shell are prepared by the co-reprecipitation method.
  • MCSe 2-(3-octadecyl-2-benzoselenazolinylidene) ethylidene]rhodanine
  • CuPc copper II) phthalocyanine
  • a water dispersion of Au nanoparticles was prepared by the reduction of HAuCl 4 using sodium citrate. Subsequently, 2 M NH40H (50 ⁇ l) was added and the mixture was thermally treated at 50° C. This amine treatment often stimulates the J-aggregate formation of MCSe.6
  • Ag—CuPc and Au—CuPc nanocomplexes were also fabricated in the same manner: 1 mM 1-methyl-2-pyrrolidinone (NMP) solution of CuPc (200 ⁇ l) was injected into a water dispersion (10 ml) of Ag or Au nanoparticles.
  • NMP 1-methyl-2-pyrrolidinone
  • the present invention treatment may also be used for inducing an auto vaccine effect for malignant cells, including those in solid tumors.
  • any rapidly dividing cells or stem cells may be damaged by a systemic treatment, then it may be preferable to direct the stimulating energy directly toward the tumor, preventing damage to most normal, healthy cells or stem cells by avoiding photoactivation or resonant energy transfer of the photoactivatable agent.
  • a treatment may be applied that slows or pauses mitosis.
  • a treatment is capable of slowing the division of rapidly dividing healthy cells or stem cells during the treatment, without pausing mitosis of cancerous cells.
  • a blocking agent is administered preferentially to malignant cells prior to administering the treatment that slows mitosis.
  • an aggressive cell proliferation disorder has a much higher rate of mitosis, which leads to selective destruction of a disproportionate share of the malignant cells during even a systemically administered treatment.
  • Stem cells and healthy cells may be spared from wholesale programmed cell death, even if exposed to photoactivated agents, provided that such photoactivated agents degenerate from the excited state to a lower energy state prior to binding, mitosis or other mechanisms for creating damage to the cells of a substantial fraction of the healthy stem cells.
  • an auto-immune response may not be induced.
  • a blocking agent may be used that prevents or reduces damage to stem cells or healthy cells, selectively, which would otherwise be impaired.
  • the blocking agent is selected or is administered such that the blocking agent does not impart a similar benefit to malignant cells, for example.
  • stem cells are targeted, specifically, for destruction with the intention of replacing the stem cells with a donor cell line or previously stored, healthy cells of the patient.
  • no blocking agent is used.
  • a carrier or photosensitizer is used that specifically targets the stem cells.
  • the amount of singlet oxygen required to cause cell lysis, and thus cell death is 0.333 10 ⁇ 3 mol/liter or more, or 10 9 singlet oxygen molecules/cell or more.
  • the level of singlet oxygen production caused by the initiation energy used or activatable pharmaceutical agent upon activation be less than level needed to cause cell lysis.
  • methods in accordance with the present invention may further include adding an additive to alleviate treatment side-effects.
  • additives may include, but are not limited to, antioxidants, adjuvant, or combinations thereof.
  • psoralen is used as the activatable pharmaceutical agent
  • UV-A is used as the activating energy
  • antioxidants are added to reduce the unwanted side-effects of irradiation.
  • An advantage of the methods of the present invention is that by specifically targeting cells affected by a cell proliferation disorder, such as rapidly dividing cells, and triggering a cellular change, such as apoptosis, in these cells in situ, the immune system of the host may be stimulated to have an immune response against the diseased cells. Once the host's own immune system is stimulated to have such a response, other diseased cells that are not treated by the activatable pharmaceutical agent may be recognized and be destroyed by the host's own immune system. Such autovaccine effects may be obtained, for example, in treatments using psoralen and UV-A.
  • the present invention methods can be used alone or in combination with other therapies for treatment of cell proliferation disorders. Additionally, the present invention methods can be used, if desired, in conjunction with recent advances in chronomedicine, such as that detailed in Giacchetti et al, Journal of Clinical Oncology, Vol 24, No 22 (August 1), 2006: pp. 3562-3569. In chronomedicine it has been found that cells suffering from certain types of disorders, such as cancer, respond better at certain times of the day than at others. Thus, chronomedicine could be used in conjunction with the present methods in order to augment the effect of the treatments of the present invention.
  • the present invention further provides systems and kits for practicing the above described methods.
  • a system in accordance with the present invention may include: (1) an initiation energy source; (2) one or more energy modulation agents; and (3) one or more activatable pharmaceutical agents.
  • a system in accordance with the present invention may include an initiation energy source and one or more activatable pharmaceutical agents.
  • the initiation energy source may be a linear accelerator equipped with image guided computer-control capability to deliver a precisely calibrated beam of radiation to a pre-selected coordinate.
  • linear accelerators are the SmartBeamTM IMRT (intensity modulated radiation therapy) system from Varian medical systems (Varian Medical Systems, Inc., Palo Alto, Calif.)
  • endoscopic or laproscopic devices equipped with appropriate initiation energy emitter may be used as the initiation energy source.
  • the initiation energy may be navigated and positioned at the pre-selected coordinate to deliver the desired amount of initiation energy to the site.
  • dose calculation and robotic manipulation devices may also be included in the system.
  • kits to facilitate application of the present invention.
  • a kit including a psoralen, and fractionating containers for easy fractionation and isolation of autovaccincs is contemplated.
  • kit would comprise at least one activatable pharmaceutical agent capable of causing a predetermined cellular change, at least one energy modulation agent capable of activating the at least one activatable agent when energized, at least one plasmonics agent and containers suitable for storing the agents in stable form, and preferably further comprising instructions for administering the at least one activatable pharmaceutical agent, at least one plasmonics agent and at least one energy modulation agent to a subject, and for applying an initiation energy from an initiation energy source to activate the activatable pharmaceutical agent.
  • the instructions could be in any desired form, including but not limited to, printed on a kit insert, printed on one or more containers, as well as electronically stored instructions provided on an electronic storage medium, such as a computer readable storage medium. Also optionally included is a software package on a computer readable storage medium that permits the user to integrate the information and calculate a control dose, to calculate and control intensity of the irradiation source.
  • Silver (or gold) colloids were prepared according to the standard Lee-Meisel method: 200 mL of 10 ⁇ 3 M AgNO 3 aqueous solution was boiled under vigorous stirring, then 5 mL of 35-mM sodium citrate solution were added and the resulting mixture was kept boiling for 1 h. This procedure was reported to yield ⁇ 10 11 particles/mL of homogenously sized colloidal particles with a diameter of ⁇ 35-50 nm and an absorption maximum at 390 nm. The colloidal solutions were stored at 4° C. and protected from room light. Further dilutions of the colloidal solutions were carried out using distilled water.
  • Nanospheres spin-coated on a solid support in order to produce and control the desired roughness.
  • the nanostructured support is subsequently covered with a layer of silver that provides the conduction electrons required for the surface plasmon mechanisms.
  • simple nanomaterials such as Teflon or latex nanospheres
  • Teflon and latex nanospheres are commercially available in a wide variety of sizes. The shapes of these materials are very regular and their size can be selected for optimal enhancement.
  • These materials comprise isolated dielectric nanospheres (30-nm diameter) coated with silver producing systems of half-nanoshells, referred to as nanocaps.
  • FIG. 24 shows a scanning electron micrograph (SEM) of 300-nm diameter polymer nanospheres covered by a 100-nm thick silver nanocaps (half-nanoshell) coating.
  • the nanoparticles can be sonicated to release them from the underlying substrate.
  • the effect of the sphere size and metal layer thickness upon the SERS effect can be easily investigated.
  • the silver coated nanospheres were found to be among the most plasmonics-active investigated.
  • Gold can also be used instead of silver to coat over nanoparticles comprising PA drug molecules.
  • Gold nanoshells have been prepared using the method described by Hirsch et al. [Hirsch L R, Stafford R J, Bankson J A, Sershen S R, Price R E, Hazle J D, Halas N J, West J L (2003) Nanoshell-mediated near infrared thermal therapy of tumors under MR Guidance. Proc Natl Acad Sci 100: 13549-13554] using a mechanism involving nucleation and then successive growth of gold nanoparticles around a silica dielectric core. Gold nanoparticles, the seed, prepared as described above using the Frens method, were used to grow the gold shell.
  • Silica nanoparticles (100 nm) used for the core of the nanoshells were monodispersed in solution of 1% APTES in EtOH.
  • the gold “seed” colloid synthesized using the Frens method were grown onto the surface of silica nanoparticles via molecular linkage of amine groups.
  • the “seed” covers the aminated silica nanoparticle surface, first as a discontinuous gold metal layer gradually growing forming a continuous gold shell.
  • Gold nanoparticles used as the “seed” were characterized using optical transmission spectroscopy (UV-Vis Spectrophotometer, Beckman Coulter, Fullerton, Calif.) and atomic force microscopy (Atomic Force Microscope, Veeco Instruments, Woodbury, N.Y.) while gold nanoshells were characterized using optical transmission spectroscopy and scanning electron microscopy (Scanning Electron Microscope, Hitachi S-4700, Hitachi High Technologies America, Inc. Pleasanton, N.Y.).
  • a method has been developed using nanosensors that can be used to evaluate the effectiveness of PEPST probes. Although one can use conventional methods (not requiring nanosensors), we describe the nanosensor method previously developed [P. M. Kasili, J. M. Song, and T Vo-Dinh, “ Optical Sensor for the Detection of Caspase -9 Activity in a Single Cell”, J. Am. Chem. Soc., 126, 2799-2806 (2004)].
  • the method comprises measuring caspases activated by apoptosis induced by the photoactive drugs. In this experiment, we measure two sets of cells I and II. Set I is treated with the drug ALA and set II is treated by the drug ALA conjugated to a PEPST probe described in the previous section. By comparing the results (amount of Caspases detected), one can evaluate the efficiency of the PEPST-ALA drug compared to ALA alone.
  • caspases are divided into initiator caspases and effector caspases according to their function and their sequence of activation.
  • Initiator caspases include caspase-8, -9, while effector caspases include, caspases-3, -6 and -7.
  • the activation of caspases is one of the earliest biomarkers of apoptosis making caspases an early and ideal target for measuring apoptosis.
  • Apoptosis, or programmed cell death is a mode of cell death characterized by specific morphological and biochemical features. The results obtained in these experiments can be used to evaluate the effectiveness of phototherapeutic drugs that induce apoptosis (e.g. PDT drugs).
  • tetrapeptide-based optical nanosensors were used to determine their role in response to a photodynamic therapy (PDT) agent, ⁇ -aminolevulinic acid (ALA) in the well-characterized human breast carcinoma cell line, MCF-7.
  • PDT photodynamic therapy
  • ALA ⁇ -aminolevulinic acid
  • MCF-7 MCF-7 cells were exposed to the photosensitizer ALA to explore A LA-PDT induced apoptosis by monitoring caspase-9 and caspase-7 activity.
  • Caspase-9 and caspase-7 protease activity was assessed in single living MCF-7 cells with the known caspase-9 and methylcoumarin caspase-7 substrates, Leucine-aspartic-histidine-glutamic acid 7-amino-4-methylcoumarin (LEHD-AMC) and aspartic-glutamic acid-valine-aspartic acid 7-amino-4-methylcoumarin (DEVD-AMC) respectively, covalently immobilized to the nanotips of optical nanosensors.
  • LHD-AMC Leucine-aspartic-histidine-glutamic acid 7-amino-4-methylcoumarin
  • DEVD-AMC aspartic-glutamic acid-valine-aspartic acid 7-amino-4-methylcoumarin
  • ⁇ -aminolevulinic acid ALA
  • PBS phosphate buffered saline
  • HCl hydrochloric acid
  • HNO 3 nitric acid
  • GOPS Glycidoxypropyltrimethoxysilane
  • CDI 1,1′-Carbonyldiimidazole
  • anhydrous acetonitrile purchased from Sigma-Aldrich, St. Louis, Mo.
  • Caspase-9 substrate LEHD-7-amino-4-methylcoumarin (AMC), Caspase-7 substrate, DEVD-7-amino-4-methylcoumarin (AMC), 2 ⁇ reaction buffer, dithiothreitol (DTT), and dimethylsulfoxicle (DMSO) were purchased from BD Biosciences, Palo Alto. Calif.
  • MCF-7 Human breast cancer cell line, MCF-7, was obtained from American Type Culture Collection (Rockville, Md., USA, Cat-no, HTB22). MCF-7 cells were grown in Dulbecco's Modified Eagle's Medium ((DMEM) (Mediatech, Inc., Herndon, Va.)) supplemented with 1 mM L-glutamine (Gibco, Grand Island, N.Y.) and 10% fetal bovine serum (Gibco, Grand Island, N.Y.). Cell culture was established in growth medium (described above) in standard T25 tissue culture flasks (Corning, Corning, N.Y.). The flasks were incubated in a humidified incubator at 37° C., 5% CO 2 and 86% humidity.
  • DMEM Dulbecco's Modified Eagle's Medium
  • the MCF-7 cells were studied as four separate groups with the first group, Group I, being the experimental, exposed to 0.5 mM ALA for 3 h followed by photoactivation ([+]ALA[+]PDT). This involved incubating the cells at 37° C. in 5% CO 2 for 3 h with 0.5 mM ALA. Following incubation the MCF-7 cells were exposed to red light from a HeNe laser ( ⁇ 632.8 nm, ⁇ 15 mW, Melles Griot, Carlsbad, Calif.) positioned about 5.0 cm above the cells for five minutes at a fluence of 5.0 mJ/cm 2 to photoactivate ALA and subsequently induce apoptosis.
  • a HeNe laser ⁇ 632.8 nm, ⁇ 15 mW, Melles Griot, Carlsbad, Calif.
  • this process involved cutting and polishing plastic clad silica (PCS) fibers with a 600- ⁇ m-size core (Fiberguide Industries, Stirling, N.J.).
  • PCS plastic clad silica
  • the fibers were pulled to a final tip diameter of 50 nm and then coated with ⁇ 100 nm of silver metal (99.999% pure) using a thermal evaporation deposition system (Cooke Vacuum Products, South Norwalk, Conn.) achieving a final diameter of 150 nm.
  • the fused silica nanotips were acid-cleaned (HNO 3 ) followed by several rinses with distilled water. Finally, the optical nanofibers were allowed to air dry at room temperature in a dust free environment.
  • the nanotips were then silanized and treated with an organic coupling agent, 10% Glycidoxypropyltrimethoxysilane (GOPS) in distilled water.
  • the silanization agent covalently binds to the silica surface of the nanotips modifying the hydroxyl group to a terminus that is compatible with the organic cross-linking reagent, 1′1, Carbonyldiimidazole (CDI).
  • CDI Carbonyldiimidazole
  • the use of CDI for activation introducing an imidazole-terminal group was particularly attractive since the protein to be immobilized could be used without chemical modification. Proteins bound using this procedure remained securely immobilized during washing or subsequent manipulations in immunoassay procedures, as opposed to procedures that use adsorption to attach proteins.
  • silanized and activated nanotips for measuring caspase-9 activity were immersed in a solution containing DMSO, 2 ⁇ reaction buffer, PBS, and LEHD-AMC, and allowed to incubate for 3 h at 37° C.
  • those for measuring caspase-7 activity were immersed in a solution containing DMSO, 2 ⁇ reaction buffer, PBS, and DEVD-AMC, and allowed to incubate for 3 h at 37° C.
  • the Nikon Diaphot 300 inverted microscope was equipped with a Diaphot 300/Diaphot 200 Incubator to maintain the cell cultures at 37° C. on the microscope stage, during these experiments.
  • the micromanipulation equipment consisted of MN-2 (Narishige Co. Ltd., Tokyo, Japan) Narishige three-dimensional manipulators for coarse adjustment, and Narishige MMW-23 three-dimensional hydraulic micromanipulators for fine adjustments.
  • the optical nanosensor was mounted on a micropipette holder (World Precision Instruments, Inc., Sarasota, Fla.).
  • the 325 nm laser line of a HeCd laser was focused onto a 600- ⁇ m-delivery fiber that is terminated with a subminiature A (SMA) connector.
  • the enzyme substrate-based optical nanosensor was coupled to the delivery fiber through the SMA connector and secured to the Nikon inverted microscope with micromanipulators.
  • a Hamamatsu PMT detector assembly (HC125-2) was mounted in the front port of the Diaphot 300 microscope.
  • the fluorescence emitted by AMC from the measurement made using single live cells was collected by the microscope objective and passed through a 330-380 nm filter set and then focused onto a PMT for detection.
  • the output from the PMT was recorded using a universal counter interfaced to a personal computer (PC) for data treatment and processing.
  • PC personal computer
  • group (I) ⁇ [+]ALA[+]PDT, group II ⁇ [+]ALA[ ⁇ ]PDT, group III ⁇ [ ⁇ ]ALA[+]PDT, and group IV ⁇ [ ⁇ ]ALA[ ⁇ ]PDT, MCF-7 cells were washed with PBS solution, pH 7.4, and then resuspended in lysis buffer (100 mM HEPES, pH 7.4, 10% sucrose, 0.1% 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS), 1 mM EDTA, 10 mM dithiothreitol (DTT), 1 mM phenylmethylsulphonyl fluoride (PMSF), 10 mg/ml pepstatin, 10 mg/ml leupeptin) and left on ice for 45 minutes.
  • lysis buffer 100 mM HEPES, pH 7.4, 10% sucrose, 0.1% 3-[(3-cholamidopropyl)-dimethylammoni
  • the cells were then repeatedly passed through a syringe with a 25-gauge needle until most of the cell membrane was disrupted, and centrifuged at 1500 RPM for 10 min.
  • Activity of caspases was measured using the fluorogenic substrate peptides; LEHD-AMC for caspase-9 and DEVD-AMC for caspase-7.
  • the release of AMC was measured after incubating optical nanosensors in picofuge tubes containing the cell lysates from the various treatment groups and using a HeCd laser (excitation 325 nm) to excite AMC.
  • Caspase activity was expressed as fluorescence intensity of AMC as a function of equivalent nanomoles of LEHD-AMC and DEVD-AMC respectively.
  • Caspase-7 activity was determined by incubation in cell lysate ( ⁇ 10 5 cells) obtained from the following treatment groups I, II, III, and IV. The release of AMC was measured after excitation using a HeCd laser (325 nm) and collecting the fluorescence signal using a 380 nm longpass filter.

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US14/603,539 US9439897B2 (en) 2007-11-06 2015-01-23 Use of psoralen derivatives and combination therapy for treatment of cell proliferation disorders
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Cited By (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070054319A1 (en) * 2005-07-22 2007-03-08 Boyden Edward S Light-activated cation channel and uses thereof
US20080085265A1 (en) * 2005-07-22 2008-04-10 Schneider M B System for optical stimulation of target cells
US20090088680A1 (en) * 2005-07-22 2009-04-02 Alexander Aravanis Optical tissue interface method and apparatus for stimulating cells
US20090099038A1 (en) * 2005-07-22 2009-04-16 Karl Deisseroth Cell line, system and method for optical-based screening of ion-channel modulators
US20090112133A1 (en) * 2007-10-31 2009-04-30 Karl Deisseroth Device and method for non-invasive neuromodulation
US20090177107A1 (en) * 2005-04-13 2009-07-09 Marie A. Guion-Johnson Detection of coronary artery disease using an electronic stethoscope
US20100004623A1 (en) * 2008-03-27 2010-01-07 Angiodynamics, Inc. Method for Treatment of Complications Associated with Arteriovenous Grafts and Fistulas Using Electroporation
US20100022892A1 (en) * 2008-07-23 2010-01-28 Snu R&Db Foundation Method and device for neural user interface and brain activity measuring device for the same
US20100152715A1 (en) * 2008-12-14 2010-06-17 Pattanam Srinivasan Method for Deep Tissue Laser Treatments Using Low Intensity Laser Therapy Causing Selective Destruction of Nociceptive Nerves
US20100190229A1 (en) * 2005-07-22 2010-07-29 Feng Zhang System for optical stimulation of target cells
US20110022129A1 (en) * 2007-11-05 2011-01-27 Prud Homme Robert K Nanoparticles for photodynamic therapy
US20110021970A1 (en) * 2007-11-06 2011-01-27 Duke University Non-invasive energy upconversion methods and systems for in-situ photobiomodulation
US20110137210A1 (en) * 2009-12-08 2011-06-09 Johnson Marie A Systems and methods for detecting cardiovascular disease
US20110159562A1 (en) * 2008-06-17 2011-06-30 Karl Deisseroth Apparatus and methods for controlling cellular development
US20110166632A1 (en) * 2008-07-08 2011-07-07 Delp Scott L Materials and approaches for optical stimulation of the peripheral nervous system
US20110166560A1 (en) * 2010-01-07 2011-07-07 Solar System Beauty Corporation Skin care laser device
US20110172653A1 (en) * 2008-06-17 2011-07-14 Schneider M Bret Methods, systems and devices for optical stimulation of target cells using an optical transmission element
US20110220172A1 (en) * 2007-11-06 2011-09-15 Pacific Integrated Energy, Inc. Photo induced enhanced field electron emission collector
WO2011163646A2 (en) * 2010-06-25 2011-12-29 Marek Malecki Methods for detection, diagnosis and selective eradication of neoplasms and circulating tumor cells using multidomain biotags
US8192429B2 (en) 2010-06-29 2012-06-05 Theravant, Inc. Abnormality eradication through resonance
US20120165904A1 (en) * 2010-11-22 2012-06-28 Jin Hyung Lee Optogenetic magnetic resonance imaging
WO2012102816A1 (en) * 2011-01-24 2012-08-02 Actium BioSystems, LLC System for correlating energy field characteristics with target particle characteristics in a living organism
US20120239121A1 (en) * 2011-03-15 2012-09-20 Jaerverud Karin Method of reducing the occurrence of arrhythmias via photobiomodulation and apparatus for same
US20140088487A1 (en) * 2010-12-29 2014-03-27 Ethicon Endo-Surgery, Inc. Methods and devices for activating brown adipose tissue with light
US8729040B2 (en) 2008-05-29 2014-05-20 The Board Of Trustees Of The Leland Stanford Junior University Cell line, system and method for optical control of secondary messengers
US8757166B2 (en) 2011-01-24 2014-06-24 Actium BioSystems, LLC System for defining energy field characteristics to illuminate nano-particles used to treat invasive agents
US8770203B2 (en) 2008-07-14 2014-07-08 Immunolight, Llc. Advanced methods and systems for treating cell proliferation disorders
US8802154B2 (en) 2010-08-27 2014-08-12 Sienna Labs, Inc. Thermal treatment of a pilosebaceous unit with nanoparticles
US8815582B2 (en) 2008-04-23 2014-08-26 The Board Of Trustees Of The Leland Stanford Junior University Mammalian cell expressing Volvox carteri light-activated ion channel protein (VChR1)
US20140254752A1 (en) * 2013-03-07 2014-09-11 Farida A. Selim Luminescence based spectrometers
US8864805B2 (en) 2007-01-10 2014-10-21 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US20140350534A1 (en) * 2013-02-20 2014-11-27 Sloan-Kettering Institute For Cancer Research Raman based ablation/resection systems and methods
US20140356897A1 (en) * 2011-12-08 2014-12-04 The Washington University In vivo label-free histology by photoacoustic microscopy of cell nuclei
US8932562B2 (en) 2010-11-05 2015-01-13 The Board Of Trustees Of The Leland Stanford Junior University Optically controlled CNS dysfunction
US8968171B2 (en) 2011-01-24 2015-03-03 Endomagnetics Limited System for correlating energy field characteristics with target particle characteristics in the application of an energy field to a living organism for imaging and treatment of invasive agents
US20150083579A1 (en) * 2012-04-06 2015-03-26 The Regents Of The University Of California Geometry enhancement of nanoscale energy deposition by x-rays
US20150168239A1 (en) * 2013-12-12 2015-06-18 The Board Of Trustees Of The University Of Illinois Nanoindenter Multimodal Microscope Objective for Mechanobiology
US9079940B2 (en) 2010-03-17 2015-07-14 The Board Of Trustees Of The Leland Stanford Junior University Light-sensitive ion-passing molecules
US20150265397A1 (en) * 2014-03-21 2015-09-24 Carl Zeiss Meditec Ag Method for transplanting a part of the cornea and a surgical microscope therefor
US9174190B2 (en) 2007-04-08 2015-11-03 Immunolight, Llc Plasmonic assisted systems and methods for interior energy-activation from an exterior source
US20150320599A1 (en) * 2011-06-24 2015-11-12 The Regents Of The University Of California Nonlinear optical photodynamic therapy (nlo-pdt) of the cornea
US9212294B2 (en) 2012-10-11 2015-12-15 Nanocomposix, Inc. Silver nanoplate compositions and methods
US20160015997A1 (en) * 2010-02-21 2016-01-21 C Laser, Inc. Treatment Using Low Intensity Laser Therapy
US9267889B1 (en) * 2011-10-12 2016-02-23 Stc.Unm High efficiency light absorbing and light emitting nanostructures
WO2016028680A1 (en) * 2014-08-18 2016-02-25 Immunolight, Llc. Non-invasive systems and methods for selective activation of photoreactive responses
US20160067354A1 (en) * 2014-08-29 2016-03-10 University Of South Carolina Preparations of gold/mesoporous silica hybrid nanoparitcle and applications
US9284353B2 (en) 2007-03-01 2016-03-15 The Board Of Trustees Of The Leland Stanford Junior University Mammalian codon optimized nucleotide sequence that encodes a variant opsin polypeptide derived from Natromonas pharaonis (NpHR)
US9309296B2 (en) 2008-11-14 2016-04-12 The Board Of Trustees Of The Leland Stanford Junior University Optically-based stimulation of target cells and modifications thereto
EP3006014A1 (en) * 2010-09-14 2016-04-13 L'oreal Cosmetic composition comprising a dyestuff, said dyestuff and cosmetic treatment process
US9340589B2 (en) 2010-11-05 2016-05-17 The Board Of Trustees Of The Leland Stanford Junior University Light-activated chimeric opsins and methods of using the same
US9348078B2 (en) 2010-06-08 2016-05-24 Pacific Integrated Energy, Inc. Optical antennas with enhanced fields and electron emission
US9352040B2 (en) 2007-04-08 2016-05-31 Immunolight, Llc. Methods and systems for treating cell proliferation disorders
US9365628B2 (en) 2011-12-16 2016-06-14 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
US9410007B2 (en) 2012-09-27 2016-08-09 Rhodia Operations Process for making silver nanostructures and copolymer useful in such process
CN105919592A (zh) * 2016-05-20 2016-09-07 北京普康大健康管理服务中心 一种schl量子生物能量全息分析系统和分析方法
US20160287893A1 (en) * 2005-09-26 2016-10-06 DePuy Synthes Products, Inc. Red light implants for treating osteoporosis
US20160356900A1 (en) * 2012-10-16 2016-12-08 Nanoptics, Incorporated Method and apparatus for neutron detection utilizing pulse height discrimination and pulse shape discrimination
US9522288B2 (en) 2010-11-05 2016-12-20 The Board Of Trustees Of The Leland Stanford Junior University Upconversion of light for use in optogenetic methods
US20170007847A1 (en) * 2015-07-08 2017-01-12 Wave Force Electronics Inc. Bioresonance frequency emitting device, system, and method
US20170038284A1 (en) * 1998-10-23 2017-02-09 Babak Nemati Systems for augmenting optical transmission through biological tissues
US9572880B2 (en) 2010-08-27 2017-02-21 Sienna Biopharmaceuticals, Inc. Ultrasound delivery of nanoparticles
US9592405B2 (en) 2005-04-14 2017-03-14 Photospectra Health Sciences, Inc. Ophthalmic phototherapy device and associated treatment method
US9636380B2 (en) 2013-03-15 2017-05-02 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of inputs to the ventral tegmental area
US9682247B2 (en) 2011-08-26 2017-06-20 Endomagnetics Limited Apparatus for the generation of an energy field for the treatment of cancer in body cavities and parts that are cavity-like
US9693692B2 (en) 2007-02-14 2017-07-04 The Board Of Trustees Of The Leland Stanford Junior University System, method and applications involving identification of biological circuits such as neurological characteristics
US9693825B2 (en) 2008-12-14 2017-07-04 C Laser, Inc. Fiber embedded hollow needle for percutaneous delivery of laser energy
WO2017132639A1 (en) * 2016-01-30 2017-08-03 The Board Of Trustees Of The Leland Stanford Junior University Light-activated anchoring of therapeutic factors to tissues
US9757196B2 (en) 2011-09-28 2017-09-12 Angiodynamics, Inc. Multiple treatment zone ablation probe
US9782604B2 (en) 2005-04-14 2017-10-10 Photospectra Health Sciences, Inc. Ophthalmic phototherapy device and associated treatment method
WO2017189506A1 (en) * 2016-04-25 2017-11-02 Immunolight, Llc Insertion devices and systems for production of emitted light internal to a medium and methods for their use
EP3134122A4 (en) * 2014-04-22 2017-12-20 Immunolight, Llc. Tumor imaging using photon-emitting phosphors having therapeutic properties
WO2017218537A1 (en) * 2016-06-15 2017-12-21 Arizona Board Of Regents On Behalf Of Arizona State University Prodrug and profluorescent compounds for selective mitochondrial imaging and therapeutic targeting
US9888956B2 (en) 2013-01-22 2018-02-13 Angiodynamics, Inc. Integrated pump and generator device and method of use
US9895189B2 (en) 2009-06-19 2018-02-20 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
US9992981B2 (en) 2010-11-05 2018-06-12 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of reward-related behaviors
EP3244928A4 (en) * 2015-01-14 2018-08-01 Immunolight, Llc. Non-invasive systems and methods for treatment of a host carrying a virus with photoactivatable drugs
US20180229299A1 (en) * 2017-02-15 2018-08-16 The Board Of Trustees Of The University Of Arkansas Copper-silica core-shell nanoparticles and methods
US10086012B2 (en) 2010-11-05 2018-10-02 The Board Of Trustees Of The Leland Stanford Junior University Control and characterization of memory function
US10105456B2 (en) 2012-12-19 2018-10-23 Sloan-Kettering Institute For Cancer Research Multimodal particles, methods and uses thereof
US10124186B2 (en) 2011-01-24 2018-11-13 Endomagnetics Limited System for automatically amending energy field characteristics in the application of an energy field to a living organism for treatment of invasive agents
US10206742B2 (en) 2010-02-21 2019-02-19 C Laser, Inc. Fiber embedded hollow spikes for percutaneous delivery of laser energy
US10219944B2 (en) 2014-09-09 2019-03-05 LumiThera, Inc. Devices and methods for non-invasive multi-wavelength photobiomodulation for ocular treatments
US10220092B2 (en) 2013-04-29 2019-03-05 The Board Of Trustees Of The Leland Stanford Junior University Devices, systems and methods for optogenetic modulation of action potentials in target cells
EP3365017A4 (en) * 2015-10-19 2019-04-17 Immunolight, LLC X-PACT ACTIVATED ANTI-CANCER TREATMENT BASED ON X-RAY-ACTIVATED PSORALENE
US10307609B2 (en) 2013-08-14 2019-06-04 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for controlling pain
US10322194B2 (en) 2012-08-31 2019-06-18 Sloan-Kettering Institute For Cancer Research Particles, methods and uses thereof
US10364227B2 (en) 2015-02-17 2019-07-30 Arizona Board Of Regents On Behalf Of Arizona State University Therapeutic compounds
US10426970B2 (en) 2007-10-31 2019-10-01 The Board Of Trustees Of The Leland Stanford Junior University Implantable optical stimulators
US10426844B2 (en) 2008-05-20 2019-10-01 University Of Florida Research Foundation, Incorporated Capsid-mutated rAAV vectors and methods of use
US20190299021A1 (en) * 2016-08-05 2019-10-03 Nagasaki Method & Co., Ltd. HEAD PHOTIC STIMULATION DEVICE, HEAD PHOTIC STIMULATION METHOD, AND PROGRAM [As Amended]
US10441810B2 (en) 2007-04-08 2019-10-15 Immunolight, Llc X-ray psoralen activated cancer therapy (X-PACT)
US10472340B2 (en) 2015-02-17 2019-11-12 Arizona Board Of Regents On Behalf Of Arizona State University Substituted phenothiazines as mitochondrial agents
US10568307B2 (en) 2010-11-05 2020-02-25 The Board Of Trustees Of The Leland Stanford Junior University Stabilized step function opsin proteins and methods of using the same
US10568516B2 (en) 2015-06-22 2020-02-25 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging and/or optogenetic control of light-responsive neurons
US10596387B2 (en) 2007-04-08 2020-03-24 Immunolight, Llc. Tumor imaging with X-rays and other high energy sources using as contrast agents photon-emitting phosphors having therapeutic properties
US10688202B2 (en) 2014-07-28 2020-06-23 Memorial Sloan-Kettering Cancer Center Metal(loid) chalcogen nanoparticles as universal binders for medical isotopes
US20200196639A1 (en) * 2013-03-15 2020-06-25 Immunolight, Llc. Phosphor composition having selected surface coatings
US20200222713A1 (en) * 2019-01-15 2020-07-16 Nadia Ansari Systems and Methods for the Automated Delivery of Photobiomodulation Therapy to a Patient
WO2020180425A1 (en) * 2019-03-04 2020-09-10 Immunolight, Llc. Energy augment structures for use with energy emitters and collectors
US10888227B2 (en) 2013-02-20 2021-01-12 Memorial Sloan Kettering Cancer Center Raman-triggered ablation/resection systems and methods
US10912947B2 (en) 2014-03-04 2021-02-09 Memorial Sloan Kettering Cancer Center Systems and methods for treatment of disease via application of mechanical force by controlled rotation of nanoparticles inside cells
US10919089B2 (en) 2015-07-01 2021-02-16 Memorial Sloan Kettering Cancer Center Anisotropic particles, methods and uses thereof
US10974064B2 (en) 2013-03-15 2021-04-13 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of behavioral state
US11103723B2 (en) 2012-02-21 2021-08-31 The Board Of Trustees Of The Leland Stanford Junior University Methods for treating neurogenic disorders of the pelvic floor
US11273283B2 (en) 2017-12-31 2022-03-15 Neuroenhancement Lab, LLC Method and apparatus for neuroenhancement to enhance emotional response
US11294165B2 (en) 2017-03-30 2022-04-05 The Board Of Trustees Of The Leland Stanford Junior University Modular, electro-optical device for increasing the imaging field of view using time-sequential capture
US11331019B2 (en) 2017-08-07 2022-05-17 The Research Foundation For The State University Of New York Nanoparticle sensor having a nanofibrous membrane scaffold
US11345833B2 (en) * 2014-03-18 2022-05-31 Immunolight, Llc Adhesive bonding composition and method of use
US11364361B2 (en) 2018-04-20 2022-06-21 Neuroenhancement Lab, LLC System and method for inducing sleep by transplanting mental states
US11390605B2 (en) 2016-08-25 2022-07-19 Arizona Board Of Regents On Behalf Of Arizona State University Substituted pyrimidine compounds as multifunctional radical quenchers and their uses
US11452839B2 (en) 2018-09-14 2022-09-27 Neuroenhancement Lab, LLC System and method of improving sleep
US11484731B2 (en) 2017-11-09 2022-11-01 International Business Machines Corporation Cognitive optogenetics probe and analysis
US11571587B2 (en) * 2008-03-11 2023-02-07 Immunolight, Llc. Method for treating a disease, disorder, or condition using inhalation to administer an activatable pharmaceutical agent, an energy modulation agent, or both
US20230173299A1 (en) * 2020-05-05 2023-06-08 Lumeda Inc. Time mulitplexed dosimetry system and method
US11707629B2 (en) 2009-05-28 2023-07-25 Angiodynamics, Inc. System and method for synchronizing energy delivery to the cardiac rhythm
US11717686B2 (en) 2017-12-04 2023-08-08 Neuroenhancement Lab, LLC Method and apparatus for neuroenhancement to facilitate learning and performance
US11723710B2 (en) 2016-11-17 2023-08-15 Angiodynamics, Inc. Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode
US11723579B2 (en) 2017-09-19 2023-08-15 Neuroenhancement Lab, LLC Method and apparatus for neuroenhancement
US11771763B2 (en) 2010-04-05 2023-10-03 Eos Neuroscience, Inc. Methods and compositions for decreasing chronic pain
US11844605B2 (en) 2016-11-10 2023-12-19 The Research Foundation For Suny System, method and biomarkers for airway obstruction
US11931096B2 (en) 2010-10-13 2024-03-19 Angiodynamics, Inc. System and method for electrically ablating tissue of a patient
US11992697B2 (en) 2022-03-21 2024-05-28 Immunolight, Llc X-ray psoralen activated cancer therapy (X-PACT)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ303355B6 (cs) * 2011-01-06 2012-08-08 Centrum organické chemie s.r.o. Zpusob inaktivace patogenních prionu, fotosenzitizátor pro inaktivaci patogenních prionu a použití fotosenzitizátoru pro inaktivaci patogenních prionu
CA2837823C (en) * 2011-05-31 2023-05-16 Clarencew Pty. Ltd Methods for preventing and treating motor-related neurological conditions
EP3690958A1 (en) * 2013-03-15 2020-08-05 Edward J. Britt Energy conversion device
JP2014239871A (ja) * 2013-05-07 2014-12-25 安東　秀夫 生体活動検出方法、生体活動測定装置、生体活動検出信号の転送方法および生体活動情報を利用したサービスの提供方法
BR112015030233A2 (pt) * 2013-06-05 2017-07-25 Hafezi Farhad método de aplicação de uma composição e composição farmacêutica com um regime de administração da mesma
GB2525432A (en) * 2014-04-24 2015-10-28 Univ Oslo Hf Modification of extracorporeal photopheresis technology with porphyrin precursors
FR3042874A1 (fr) * 2015-10-21 2017-04-28 Nanobacterie Particule comprenant au moins une particule d'oxyde de fer ferrimagnetique associee a au moins un compose pour une utilisation medicale ou cosmetique
AU2017249500A1 (en) * 2016-04-14 2018-11-01 Feldreich Caro Ruiz AB An apparatus for use in irradiation therapy comprising ionization module and UV-light source
CN105942978A (zh) * 2016-05-20 2016-09-21 北京普康大健康管理服务中心 一种schl量子生物能量全息检测仪和检测方法
EP3548101A4 (en) * 2016-11-30 2020-06-17 Risto Koponen METHOD AND APPARATUS FOR TRANSMITTING UV LIGHT DESTROYING MICROORGANISMS FROM A LIGHT SOURCE TO A TARGET
AU2018212954A1 (en) * 2017-01-27 2019-09-12 FB Dermatology Limited Methods for photobiomodulation of biological processes using fluorescence generated and emitted from a biophotonic composition or a biophotonic system
US10799713B2 (en) * 2017-08-14 2020-10-13 Veralase, LLC Miniature wearable laser treatment device
KR20190052241A (ko) * 2017-11-08 2019-05-16 서울바이오시스 주식회사 의료용 캡슐 장치
CN108508068B (zh) * 2018-03-27 2020-07-24 长沙理工大学 阴离子卟啉-碳纳米管修饰电极测her2基因特定序列
US20210268251A1 (en) * 2018-07-13 2021-09-02 Bard Peripheral Vascular, Inc. Implantable Ports, Implantable Port-Detecting Devices, and Methods Thereof
KR20210090630A (ko) * 2018-10-12 2021-07-20 이뮤노라이트, 엘엘씨 세포-대-세포 통신을 측정 및 유도하기 위한 방법, 장치 및 조성물, 및 이의 치료적 용도
SE2000055A1 (sv) * 2020-03-15 2021-09-16 Martin Ivanov Denev Användande av fotohydrauliskt mekanisk chock, för selektiv sprängning av relativ svagare cellmembranen av cancerceller, vilka har svagare cellmembran än friska celler
TWI764421B (zh) * 2020-12-09 2022-05-11 羅莎國際有限公司 高分子低溫離子氣體促進傷口癒合裝置
RU2754617C1 (ru) * 2021-01-11 2021-09-06 Общество С Ограниченной Ответственностью "Лаборатория Инновационных Технологий" Способ получения фармацевтического средства для торможения пролиферативной активности опухолевых клеток
CN112843089B (zh) * 2021-01-29 2021-10-29 燕山大学 一种改善肿瘤微环境的钌基抗肿瘤纳米药物的制备方法
KR102355947B1 (ko) * 2021-03-23 2022-02-08 어썸레이 주식회사 전자기파를 이용한 염증성 질환의 치료, 억제 및 예방을 위한 치료 장치 및 방법
CN113563876B (zh) * 2021-07-06 2022-09-27 江南大学 一种增强型黄光碳点及其制备方法和应用
WO2024026041A1 (en) * 2022-07-29 2024-02-01 Board Of Trustees Of Michigan State University Remote control and quantitative monitoring of drug release from nanoparticles based on magnetic particle imaging

Citations (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4111890A (en) * 1977-12-19 1978-09-05 Sws Silicones Corporation Curable organopolysiloxane compositions containing titanium esters
US4675346A (en) * 1983-06-20 1987-06-23 Loctite Corporation UV curable silicone rubber compositions
US4838852A (en) * 1987-03-27 1989-06-13 Therakos, Inc. Active specific immune suppression
US4979935A (en) * 1989-02-21 1990-12-25 Quantex Corporation Method of photodynamic therapy employing electron trapping material
US5118422A (en) * 1990-07-24 1992-06-02 Photo-Catalytics, Inc. Photocatalytic treatment of water
US5120649A (en) * 1990-05-15 1992-06-09 New York Blood Center, Inc. Photodynamic inactivation of viruses in blood cell-containing compositions
US5360734A (en) * 1991-06-21 1994-11-01 Baxter International, Inc. Method for inactivating pathogens in erythrocytes using photoactive compounds and plasma protein reduction
US5489590A (en) * 1988-09-30 1996-02-06 Baylor Research Foundation Method of treating with therapeutic composition comprising photoactive compound
US5521289A (en) * 1994-07-29 1996-05-28 Nanoprobes, Inc. Small organometallic probes
US5728590A (en) * 1994-07-29 1998-03-17 Nanoprobes, Inc. Small organometallic probes
US5786198A (en) * 1992-12-23 1998-07-28 Iowa State University Research Foundation Photoactivated antiviral and antitumor compositions
US5829448A (en) * 1996-10-30 1998-11-03 Photogen, Inc. Method for improved selectivity in photo-activation of molecular agents
US5957960A (en) * 1997-05-05 1999-09-28 Light Sciences Limited Partnership Internal two photon excitation device for delivery of PDT to diffuse abnormal cells
US5980954A (en) * 1992-02-07 1999-11-09 Vasogen Ireland Limited Treatment of autoimmune diseases
US6036941A (en) * 1995-07-19 2000-03-14 Consiglio Nazionale Delle Ricerche Fluorogenic substrates for diagnosis and photodynamic treatment of tumors
US6051625A (en) * 1996-06-27 2000-04-18 Dow Corning Asia, Ltd. Ultraviolet-curable polysiloxane composition and method for the formation of cured patterns therefrom
US6071944A (en) * 1997-11-12 2000-06-06 Bowling Green State University Method of treatment of pigmented cancer cells utilizing photodynamic therapy
US6087141A (en) * 1990-05-15 2000-07-11 New York Blood Center, Inc. Process for the sterilization of biological compositions and the product produced thereby
US6121425A (en) * 1994-07-29 2000-09-19 Nanoprobes, Inc. Metal-lipid molecules
US6204058B1 (en) * 1992-02-07 2001-03-20 Vasogen Ireland Limited Treatment of autoimmune diseases
US6235508B1 (en) * 1995-06-07 2001-05-22 Baxter International Inc. Method of inactivation of viral and bacterial blood contaminants
US6281261B1 (en) * 1998-06-01 2001-08-28 Loctite Corporation Flame-retardant UV curable silicone compositions
US6323253B1 (en) * 1998-06-01 2001-11-27 Loctite Corporation Flame-retardant UV and UV/moisture curable silicone compositions
US20020127224A1 (en) * 2001-03-02 2002-09-12 James Chen Use of photoluminescent nanoparticles for photodynamic therapy
US20030022170A1 (en) * 1998-03-06 2003-01-30 Millenium Pharmaceuticals, Inc. Novel fibroblast growth factors and therapeutic and diagnostic uses therefor
US6609014B1 (en) * 1999-04-14 2003-08-19 Qlt Inc. Use of PDT to inhibit intimal hyperplasia
US6627923B1 (en) * 1999-07-12 2003-09-30 Massachusetts Institute Of Technology Resonant microcavities
US6670113B2 (en) * 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
US6669965B2 (en) * 1992-02-07 2003-12-30 Vasogen Ireland Limited Method of treating atherosclerosis
US6719778B1 (en) * 2000-03-24 2004-04-13 Endovascular Technologies, Inc. Methods for treatment of aneurysms
US20040214001A1 (en) * 1997-03-12 2004-10-28 William Marsh Rice University Metal nanoshells
US6811562B1 (en) * 2000-07-31 2004-11-02 Epicor, Inc. Procedures for photodynamic cardiac ablation therapy and devices for those procedures
US20040253138A1 (en) * 2003-06-16 2004-12-16 American Environmental Systems, Inc. Plasmon enhanced body treatment and bacterial management
US20050020869A1 (en) * 1998-07-30 2005-01-27 Hainfeld James F. Methods of enhancing radiation effects with metal nanoparticles
US6849058B1 (en) * 1992-05-27 2005-02-01 Qlt, Inc. Photodynamic therapy in selective cell inactivation in blood and treating immune dysfunction diseases
US7008559B2 (en) * 2001-06-06 2006-03-07 Nomadics, Inc. Manganese doped upconversion luminescence nanoparticles
US20060067889A1 (en) * 2004-09-27 2006-03-30 Light Sciences Corporation Singlet oxygen photosensitizers activated by target binding enhancing the selectivity of targeted PDT agents
US20060089836A1 (en) * 2004-10-21 2006-04-27 Motorola, Inc. System and method of signal pre-conditioning with adaptive spectral tilt compensation for audio equalization
US7045124B1 (en) * 1999-01-12 2006-05-16 Vasogen Irelend Limited Pre-conditioning against cell death
US20060255292A1 (en) * 2005-01-26 2006-11-16 Nomadics, Inc. Standoff optical detection platform based on surface plasmon-coupled emission
US20070059316A1 (en) * 2003-09-23 2007-03-15 Pallenberg Alexander J Singlet oxygen photosensitizers activated by target binding enhancing the selectivity of targeted pdt agents
US20070063154A1 (en) * 2005-02-02 2007-03-22 Wei Chen Energy-transfer nanocomposite materials and methods of making and using same
US20070189359A1 (en) * 2002-06-12 2007-08-16 Wei Chen Nanoparticle thermometry and pressure sensors
US7267948B2 (en) * 1997-11-26 2007-09-11 Ut-Battelle, Llc SERS diagnostic platforms, methods and systems microarrays, biosensors and biochips
US20070217996A1 (en) * 2004-05-10 2007-09-20 Laurent Levy Activatable Particles, Preparations and Uses
US20070218049A1 (en) * 2006-02-02 2007-09-20 Wei Chen Nanoparticle based photodynamic therapy and methods of making and using same
US20070243137A1 (en) * 2006-04-18 2007-10-18 Nanoprobes, Inc. Cell and sub-cell methods for imaging and therapy
US7294656B2 (en) * 2004-01-09 2007-11-13 Bayer Materialscience Llc UV curable coating composition
US20070274909A1 (en) * 2003-12-17 2007-11-29 Koninklijke Philips Electronic, N.V. Radiation Therapy and Medical Imaging Using Uv Emitting Nanoparticles
US20070292353A1 (en) * 2004-11-05 2007-12-20 Laurent Levy Nanoparticles Comprising an Intracellular Targeting Element and Preparation and Use Thereof
US20080003183A1 (en) * 2004-09-28 2008-01-03 The Regents Of The University Of California Nanoparticle radiosensitizers
US20080039436A1 (en) * 2004-07-13 2008-02-14 Patel Bipin C M Porphyrin Derivatives And Their Use In Photon Activation Therapy
US20080089836A1 (en) * 2006-10-12 2008-04-17 Nanoprobes, Inc. Functional associative coatings for nanoparticles
US7364872B1 (en) * 2001-03-30 2008-04-29 Nanoprobes Test methods using enzymatic deposition and alteration of metals
US20080139993A1 (en) * 2006-09-18 2008-06-12 The University Of Houston System Use of nanoparticles in the photodynamic treatment of tumors and non-destructive testing
US20080248001A1 (en) * 2007-04-08 2008-10-09 Immunolight Methods and systems for treating cell proliferation disorders
US20090104212A1 (en) * 2007-08-06 2009-04-23 Immunolight Methods and systems for treating cell proliferation disorders using two-photon simultaneous absorption

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4522811A (en) 1982-07-08 1985-06-11 Syntex (U.S.A.) Inc. Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides
US4748120A (en) 1983-05-02 1988-05-31 Diamond Scientific Co. Photochemical decontamination treatment of whole blood or blood components
US4705952A (en) 1985-10-10 1987-11-10 Quantex Corporation Communications apparatus using infrared-triggered phosphor for receiving infrared signals
US5091385A (en) * 1988-09-30 1992-02-25 Baylor Research Institute Pre-activated therapeutic agents derived from photoactive compounds
US5216176A (en) 1989-01-23 1993-06-01 Lehigh University 7-alkoxycoumarins, dihydropsoralens, and benzodipyranones as photo-activated therapeutic agents and inhibitors of epidermal growth factor
US5257970A (en) 1992-04-09 1993-11-02 Health Research, Inc. In situ photodynamic therapy
JPH0867682A (ja) * 1994-08-30 1996-03-12 Toyo Hatsuka Kogyo Kk ニトロイミダゾール担持ポルフィリン誘導体とその用 途
JP3689939B2 (ja) 1995-07-28 2005-08-31 豊田合成株式会社 光触媒装置
EP0862432A4 (en) 1995-09-06 2003-03-19 Univ New York State Res Found TWO-PHOTON CONVERTERING DYES AND THEIR APPLICATIONS
CN1121217C (zh) 1996-03-29 2003-09-17 特拉科斯有限公司 免疫原性组合物
JPH09299937A (ja) 1996-05-09 1997-11-25 Daikin Ind Ltd 被処理体処理装置
WO1998004318A1 (en) * 1996-07-25 1998-02-05 Light Medicine, Inc. Photodynamic therapy with light emitting particles in bloodstream
US6887260B1 (en) 1998-11-30 2005-05-03 Light Bioscience, Llc Method and apparatus for acne treatment
KR100770768B1 (ko) 2000-04-20 2007-10-26 코닌클리케 필립스 일렉트로닉스 엔.브이. 광 기록매체와 이 광 기록매체를 이용한 기록방법
US6589948B1 (en) 2000-11-28 2003-07-08 Eukarion, Inc. Cyclic salen-metal compounds: reactive oxygen species scavengers useful as antioxidants in the treatment and prevention of diseases
US20060004347A1 (en) * 2000-12-28 2006-01-05 Palomar Medical Technologies, Inc. Methods and products for producing lattices of EMR-treated islets in tissues, and uses therefor
EP1404334A4 (en) * 2001-05-15 2005-02-02 Faulk Pharmaceuticals Inc TARGETED ADMINISTRATION OF BIOACTIVE COMPOUNDS FOR THE TREATMENT OF CANCER
JP2004532251A (ja) 2001-05-31 2004-10-21 ミラヴァント ファーマシューティカルズ インコーポレイテッド 光線力学療法で使用のメタロテトラピロール系光増感剤
GB0118251D0 (en) * 2001-07-26 2001-09-19 Photocure Asa Method
US7303578B2 (en) 2001-11-01 2007-12-04 Photothera, Inc. Device and method for providing phototherapy to the brain
EP1465699A4 (en) 2001-12-12 2006-05-24 Leon J Lewandowski AUTOIMMUNE STIMULATION BY PHOTOPHORESIS
US6908591B2 (en) 2002-07-18 2005-06-21 Clearant, Inc. Methods for sterilizing biological materials by irradiation over a temperature gradient
US7274772B2 (en) * 2004-05-27 2007-09-25 Cabot Microelectronics Corporation X-ray source with nonparallel geometry
JP2005349028A (ja) * 2004-06-11 2005-12-22 Shibuya Kogyo Co Ltd 腫瘍治療方法及びその装置
GB0415263D0 (en) * 2004-07-07 2004-08-11 Norwegian Radium Hospital Res Method
GB2416699B (en) * 2004-08-05 2010-04-14 Photo Therapeutics Ltd Skin rejuvenation
JP2008519849A (ja) * 2004-11-12 2008-06-12 ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム タンパク質−貴金属ナノ粒子
US7999161B2 (en) * 2005-01-22 2011-08-16 Alexander Oraevsky Laser-activated nanothermolysis of cells
JP2006290840A (ja) 2005-04-14 2006-10-26 Shetech:Kk 白金ナノ粒子を含有する活性酸素種除去材
IL168184A (en) * 2005-04-21 2011-11-30 Univ Ariel Res & Dev Co Ltd Use of a ligand-photosensitizer conjugate in combination with a chemiluminescent agent in the manufacture of a medicament for photodynamic therapy
EP1899732A4 (en) * 2005-05-11 2009-02-18 Georgia Tech Res Inst ACCORDABLE PLASMONIC NANOPARTICLES
US20090209508A1 (en) * 2005-05-16 2009-08-20 Universite De Geneve Compounds for Photochemotherapy
JP4751691B2 (ja) * 2005-10-12 2011-08-17 トヨタ自動車株式会社 核酸高分子の分解方法及び分解装置
EP1779891A1 (en) 2005-10-28 2007-05-02 Abdula Kurkayev Method of activating a photosensitizer
CA2644694C (en) * 2006-03-10 2014-05-13 Sangeeta N. Bhatia Triggered self-assembly conjugates and nanosystems
WO2007108512A1 (ja) 2006-03-22 2007-09-27 T.N.G. Technologies Co., Ltd. 金属コーティング材の製造方法及び金属コーティング材
JP2009544584A (ja) 2006-07-10 2009-12-17 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 治療及び画像化を目的とするコア・シェル構造ナノ粒子
GB0712287D0 (en) * 2007-06-22 2007-08-01 Ucl Business Plc Antimicrobial Conjugates
JP2008137939A (ja) * 2006-12-01 2008-06-19 National Institute Of Advanced Industrial & Technology Ｘ線治療用助剤
US8951561B2 (en) * 2007-08-06 2015-02-10 Duke University Methods and systems for treating cell proliferation disorders using plasmonics enhanced photospectral therapy (PEPST) and exciton-plasmon enhanced phototherapy (EPEP)
US8376013B2 (en) * 2008-03-11 2013-02-19 Duke University Plasmonic assisted systems and methods for interior energy-activation from an exterior source
CN101101263A (zh) * 2007-07-20 2008-01-09 苏州大学 高活性表面增强拉曼光谱的核壳纳米粒子及其制备方法
US9439897B2 (en) * 2007-11-06 2016-09-13 Immunolight, Llc Use of psoralen derivatives and combination therapy for treatment of cell proliferation disorders
ITBS20070177A1 (it) 2007-11-15 2009-05-16 Paoli Ambrosi Gianfranco De Composizione per uso topico per ottenere un rapido ed intenso effetto lifting
WO2010009106A1 (en) * 2008-07-14 2010-01-21 Bourke Frederic A Jr Advanced methods and systems for treating cell proliferation disorders

Patent Citations (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4111890A (en) * 1977-12-19 1978-09-05 Sws Silicones Corporation Curable organopolysiloxane compositions containing titanium esters
US4675346A (en) * 1983-06-20 1987-06-23 Loctite Corporation UV curable silicone rubber compositions
US4838852A (en) * 1987-03-27 1989-06-13 Therakos, Inc. Active specific immune suppression
US5489590A (en) * 1988-09-30 1996-02-06 Baylor Research Foundation Method of treating with therapeutic composition comprising photoactive compound
US4979935A (en) * 1989-02-21 1990-12-25 Quantex Corporation Method of photodynamic therapy employing electron trapping material
US6087141A (en) * 1990-05-15 2000-07-11 New York Blood Center, Inc. Process for the sterilization of biological compositions and the product produced thereby
US5120649A (en) * 1990-05-15 1992-06-09 New York Blood Center, Inc. Photodynamic inactivation of viruses in blood cell-containing compositions
US5118422A (en) * 1990-07-24 1992-06-02 Photo-Catalytics, Inc. Photocatalytic treatment of water
US5360734A (en) * 1991-06-21 1994-11-01 Baxter International, Inc. Method for inactivating pathogens in erythrocytes using photoactive compounds and plasma protein reduction
US6204058B1 (en) * 1992-02-07 2001-03-20 Vasogen Ireland Limited Treatment of autoimmune diseases
US6569467B1 (en) * 1992-02-07 2003-05-27 Vasogen Ireland Limited Treatment of autoimmune diseases
US6669965B2 (en) * 1992-02-07 2003-12-30 Vasogen Ireland Limited Method of treating atherosclerosis
US5980954A (en) * 1992-02-07 1999-11-09 Vasogen Ireland Limited Treatment of autoimmune diseases
US6849058B1 (en) * 1992-05-27 2005-02-01 Qlt, Inc. Photodynamic therapy in selective cell inactivation in blood and treating immune dysfunction diseases
US5786198A (en) * 1992-12-23 1998-07-28 Iowa State University Research Foundation Photoactivated antiviral and antitumor compositions
US6121425A (en) * 1994-07-29 2000-09-19 Nanoprobes, Inc. Metal-lipid molecules
US5728590A (en) * 1994-07-29 1998-03-17 Nanoprobes, Inc. Small organometallic probes
US5521289A (en) * 1994-07-29 1996-05-28 Nanoprobes, Inc. Small organometallic probes
US6235508B1 (en) * 1995-06-07 2001-05-22 Baxter International Inc. Method of inactivation of viral and bacterial blood contaminants
US6036941A (en) * 1995-07-19 2000-03-14 Consiglio Nazionale Delle Ricerche Fluorogenic substrates for diagnosis and photodynamic treatment of tumors
US6051625A (en) * 1996-06-27 2000-04-18 Dow Corning Asia, Ltd. Ultraviolet-curable polysiloxane composition and method for the formation of cured patterns therefrom
US6042603A (en) * 1996-10-30 2000-03-28 Photogen, Inc. Method for improved selectivity in photo-activation of molecular agents
US5829448A (en) * 1996-10-30 1998-11-03 Photogen, Inc. Method for improved selectivity in photo-activation of molecular agents
US20040214001A1 (en) * 1997-03-12 2004-10-28 William Marsh Rice University Metal nanoshells
US5957960A (en) * 1997-05-05 1999-09-28 Light Sciences Limited Partnership Internal two photon excitation device for delivery of PDT to diffuse abnormal cells
US6225333B1 (en) * 1997-11-12 2001-05-01 Bowling Green State University Method of treatment of pigmented cancer cells utilizing photodynamic therapy
US6071944A (en) * 1997-11-12 2000-06-06 Bowling Green State University Method of treatment of pigmented cancer cells utilizing photodynamic therapy
US7267948B2 (en) * 1997-11-26 2007-09-11 Ut-Battelle, Llc SERS diagnostic platforms, methods and systems microarrays, biosensors and biochips
US20030022170A1 (en) * 1998-03-06 2003-01-30 Millenium Pharmaceuticals, Inc. Novel fibroblast growth factors and therapeutic and diagnostic uses therefor
US6323253B1 (en) * 1998-06-01 2001-11-27 Loctite Corporation Flame-retardant UV and UV/moisture curable silicone compositions
US6281261B1 (en) * 1998-06-01 2001-08-28 Loctite Corporation Flame-retardant UV curable silicone compositions
US20050020869A1 (en) * 1998-07-30 2005-01-27 Hainfeld James F. Methods of enhancing radiation effects with metal nanoparticles
US7530940B2 (en) * 1998-07-30 2009-05-12 Nanoprobes, Inc. Methods of enhancing radiation effects with metal nanoparticles
US6955639B2 (en) * 1998-07-30 2005-10-18 Nanoprobes, Inc. Methods of enhancing radiation effects with metal nanoparticles
US7367934B2 (en) * 1998-07-30 2008-05-06 Nanoprobes, Inc. Methods of enhancing radiation effects with metal nanoparticles
US7045124B1 (en) * 1999-01-12 2006-05-16 Vasogen Irelend Limited Pre-conditioning against cell death
US6609014B1 (en) * 1999-04-14 2003-08-19 Qlt Inc. Use of PDT to inhibit intimal hyperplasia
US6627923B1 (en) * 1999-07-12 2003-09-30 Massachusetts Institute Of Technology Resonant microcavities
US6719778B1 (en) * 2000-03-24 2004-04-13 Endovascular Technologies, Inc. Methods for treatment of aneurysms
US6811562B1 (en) * 2000-07-31 2004-11-02 Epicor, Inc. Procedures for photodynamic cardiac ablation therapy and devices for those procedures
US20020127224A1 (en) * 2001-03-02 2002-09-12 James Chen Use of photoluminescent nanoparticles for photodynamic therapy
US7183072B1 (en) * 2001-03-30 2007-02-27 Nanoprobes, Inc. Kit for detecting Her-2/neu gene by site-specific metal deposition
US6670113B2 (en) * 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
US7364872B1 (en) * 2001-03-30 2008-04-29 Nanoprobes Test methods using enzymatic deposition and alteration of metals
US7008559B2 (en) * 2001-06-06 2006-03-07 Nomadics, Inc. Manganese doped upconversion luminescence nanoparticles
US20070189359A1 (en) * 2002-06-12 2007-08-16 Wei Chen Nanoparticle thermometry and pressure sensors
US20040253138A1 (en) * 2003-06-16 2004-12-16 American Environmental Systems, Inc. Plasmon enhanced body treatment and bacterial management
US20070059316A1 (en) * 2003-09-23 2007-03-15 Pallenberg Alexander J Singlet oxygen photosensitizers activated by target binding enhancing the selectivity of targeted pdt agents
US20070274909A1 (en) * 2003-12-17 2007-11-29 Koninklijke Philips Electronic, N.V. Radiation Therapy and Medical Imaging Using Uv Emitting Nanoparticles
US7294656B2 (en) * 2004-01-09 2007-11-13 Bayer Materialscience Llc UV curable coating composition
US20070217996A1 (en) * 2004-05-10 2007-09-20 Laurent Levy Activatable Particles, Preparations and Uses
US20080039436A1 (en) * 2004-07-13 2008-02-14 Patel Bipin C M Porphyrin Derivatives And Their Use In Photon Activation Therapy
US20060067889A1 (en) * 2004-09-27 2006-03-30 Light Sciences Corporation Singlet oxygen photosensitizers activated by target binding enhancing the selectivity of targeted PDT agents
US20080003183A1 (en) * 2004-09-28 2008-01-03 The Regents Of The University Of California Nanoparticle radiosensitizers
US20060089836A1 (en) * 2004-10-21 2006-04-27 Motorola, Inc. System and method of signal pre-conditioning with adaptive spectral tilt compensation for audio equalization
US20070292353A1 (en) * 2004-11-05 2007-12-20 Laurent Levy Nanoparticles Comprising an Intracellular Targeting Element and Preparation and Use Thereof
US20060255292A1 (en) * 2005-01-26 2006-11-16 Nomadics, Inc. Standoff optical detection platform based on surface plasmon-coupled emission
US20070063154A1 (en) * 2005-02-02 2007-03-22 Wei Chen Energy-transfer nanocomposite materials and methods of making and using same
US20070218049A1 (en) * 2006-02-02 2007-09-20 Wei Chen Nanoparticle based photodynamic therapy and methods of making and using same
US20070243137A1 (en) * 2006-04-18 2007-10-18 Nanoprobes, Inc. Cell and sub-cell methods for imaging and therapy
US20080139993A1 (en) * 2006-09-18 2008-06-12 The University Of Houston System Use of nanoparticles in the photodynamic treatment of tumors and non-destructive testing
US20080089836A1 (en) * 2006-10-12 2008-04-17 Nanoprobes, Inc. Functional associative coatings for nanoparticles
US20080248001A1 (en) * 2007-04-08 2008-10-09 Immunolight Methods and systems for treating cell proliferation disorders
US20090104212A1 (en) * 2007-08-06 2009-04-23 Immunolight Methods and systems for treating cell proliferation disorders using two-photon simultaneous absorption

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Fan et al. Assay and Drug Development Technologies, vol. 5, no. 1, pages 127-136 *
Schaffer et al. Journal of Photochemistry and Photobiology B: Biology, 2002, vol. 66, pages 157-164 *

Cited By (277)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170065345A1 (en) * 1998-10-23 2017-03-09 Babak Nemati Systems for augmenting optical transmission through biological tissues
US20170038284A1 (en) * 1998-10-23 2017-02-09 Babak Nemati Systems for augmenting optical transmission through biological tissues
US10039520B2 (en) 2005-04-13 2018-08-07 Aum Cardiovascular, Inc Detection of coronary artery disease using an electronic stethoscope
US20090177107A1 (en) * 2005-04-13 2009-07-09 Marie A. Guion-Johnson Detection of coronary artery disease using an electronic stethoscope
US9814903B2 (en) 2005-04-14 2017-11-14 Photospectra Health Services, Inc. Ophthalmic phototherapy system and associated method
US9782604B2 (en) 2005-04-14 2017-10-10 Photospectra Health Sciences, Inc. Ophthalmic phototherapy device and associated treatment method
US10252078B2 (en) 2005-04-14 2019-04-09 Photospectra Health Sciences, Inc. Ophthalmic phototherapy method
US9974971B2 (en) 2005-04-14 2018-05-22 Photospectra Health Sciences, Inc Ophthalmic phototherapy method
US9592404B2 (en) 2005-04-14 2017-03-14 Photospectra Health Sciences, Inc. Ophthalmic phototherapy device and associated treatment method
US9592405B2 (en) 2005-04-14 2017-03-14 Photospectra Health Sciences, Inc. Ophthalmic phototherapy device and associated treatment method
US9360472B2 (en) 2005-07-22 2016-06-07 The Board Of Trustees Of The Leland Stanford Junior University Cell line, system and method for optical-based screening of ion-channel modulators
US20090088680A1 (en) * 2005-07-22 2009-04-02 Alexander Aravanis Optical tissue interface method and apparatus for stimulating cells
US9278159B2 (en) 2005-07-22 2016-03-08 The Board Of Trustees Of The Leland Stanford Junior University Light-activated cation channel and uses thereof
US9274099B2 (en) 2005-07-22 2016-03-01 The Board Of Trustees Of The Leland Stanford Junior University Screening test drugs to identify their effects on cell membrane voltage-gated ion channel
US20100190229A1 (en) * 2005-07-22 2010-07-29 Feng Zhang System for optical stimulation of target cells
US10052497B2 (en) 2005-07-22 2018-08-21 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US10036758B2 (en) 2005-07-22 2018-07-31 The Board Of Trustees Of The Leland Stanford Junior University Delivery of a light-activated cation channel into the brain of a subject
US20080085265A1 (en) * 2005-07-22 2008-04-10 Schneider M B System for optical stimulation of target cells
US10094840B2 (en) 2005-07-22 2018-10-09 The Board Of Trustees Of The Leland Stanford Junior University Light-activated cation channel and uses thereof
US20100234273A1 (en) * 2005-07-22 2010-09-16 The Board Of Trustees Of The Leland Stanford Junior University Light-activated cation channel and uses thereof
US8926959B2 (en) 2005-07-22 2015-01-06 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US20070054319A1 (en) * 2005-07-22 2007-03-08 Boyden Edward S Light-activated cation channel and uses thereof
US10422803B2 (en) 2005-07-22 2019-09-24 The Board Of Trustees Of The Leland Stanford Junior University Light-activated cation channel and uses thereof
US20090099038A1 (en) * 2005-07-22 2009-04-16 Karl Deisseroth Cell line, system and method for optical-based screening of ion-channel modulators
US9238150B2 (en) 2005-07-22 2016-01-19 The Board Of Trustees Of The Leland Stanford Junior University Optical tissue interface method and apparatus for stimulating cells
US20070261127A1 (en) * 2005-07-22 2007-11-08 Boyden Edward S Light-activated cation channel and uses thereof
US10451608B2 (en) 2005-07-22 2019-10-22 The Board Of Trustees Of The Leland Stanford Junior University Cell line, system and method for optical-based screening of ion-channel modulators
US8906360B2 (en) 2005-07-22 2014-12-09 The Board Of Trustees Of The Leland Stanford Junior University Light-activated cation channel and uses thereof
US9829492B2 (en) 2005-07-22 2017-11-28 The Board Of Trustees Of The Leland Stanford Junior University Implantable prosthetic device comprising a cell expressing a channelrhodopsin
US10569099B2 (en) 2005-07-22 2020-02-25 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US10627410B2 (en) 2005-07-22 2020-04-21 The Board Of Trustees Of The Leland Stanford Junior University Light-activated cation channel and uses thereof
US9101690B2 (en) 2005-07-22 2015-08-11 The Board Of Trustees Of The Leland Stanford Junior University Light-activated cation channel and uses thereof
US10046174B2 (en) 2005-07-22 2018-08-14 The Board Of Trustees Of The Leland Stanford Junior University System for electrically stimulating target neuronal cells of a living animal in vivo
US10252077B2 (en) 2005-09-26 2019-04-09 DePuy Synthes Products, Inc. Red light implants for treating osteoporosis
US10245443B2 (en) * 2005-09-26 2019-04-02 DePuy Synthes Products, Inc. Red light implants for treating osteoporosis
US20160287893A1 (en) * 2005-09-26 2016-10-06 DePuy Synthes Products, Inc. Red light implants for treating osteoporosis
US10105551B2 (en) 2007-01-10 2018-10-23 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US8864805B2 (en) 2007-01-10 2014-10-21 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US9187745B2 (en) 2007-01-10 2015-11-17 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US10369378B2 (en) 2007-01-10 2019-08-06 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US11007374B2 (en) 2007-01-10 2021-05-18 The Board Of Trustees Of The Leland Stanford Junior University System for optical stimulation of target cells
US9693692B2 (en) 2007-02-14 2017-07-04 The Board Of Trustees Of The Leland Stanford Junior University System, method and applications involving identification of biological circuits such as neurological characteristics
US9855442B2 (en) 2007-03-01 2018-01-02 The Board Of Trustees Of The Leland Stanford Junior University Method for optically controlling a neuron with a mammalian codon optimized nucleotide sequence that encodes a variant opsin polypeptide derived from natromonas pharaonis (NpHR)
US10589123B2 (en) 2007-03-01 2020-03-17 The Board Of Trustees Of The Leland Stanford Junior University Systems, methods and compositions for optical stimulation of target cells
US9757587B2 (en) 2007-03-01 2017-09-12 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic method for generating an inhibitory current in a mammalian neuron
US9284353B2 (en) 2007-03-01 2016-03-15 The Board Of Trustees Of The Leland Stanford Junior University Mammalian codon optimized nucleotide sequence that encodes a variant opsin polypeptide derived from Natromonas pharaonis (NpHR)
US9174190B2 (en) 2007-04-08 2015-11-03 Immunolight, Llc Plasmonic assisted systems and methods for interior energy-activation from an exterior source
US10441810B2 (en) 2007-04-08 2019-10-15 Immunolight, Llc X-ray psoralen activated cancer therapy (X-PACT)
US9352040B2 (en) 2007-04-08 2016-05-31 Immunolight, Llc. Methods and systems for treating cell proliferation disorders
US9498643B2 (en) 2007-04-08 2016-11-22 Immunolight, Llc Systems and methods for interior energy-activation from an exterior source
US10398777B2 (en) 2007-04-08 2019-09-03 Immunolight, Llc Methods and systems for treating cell proliferation disorders
US9358292B2 (en) 2007-04-08 2016-06-07 Immunolight, Llc Methods and systems for treating cell proliferation disorders
US9682250B2 (en) 2007-04-08 2017-06-20 Immunolight, Llc Systems and methods for interior energy-activation from an exterior source
US9682146B2 (en) 2007-04-08 2017-06-20 Immunolight, Llc. Methods and systems for treating cell proliferation related disorders
US11103580B2 (en) 2007-04-08 2021-08-31 Immunolight, Llc Methods and systems for treating cell proliferation disorders
US10596387B2 (en) 2007-04-08 2020-03-24 Immunolight, Llc. Tumor imaging with X-rays and other high energy sources using as contrast agents photon-emitting phosphors having therapeutic properties
US10434327B2 (en) 2007-10-31 2019-10-08 The Board Of Trustees Of The Leland Stanford Junior University Implantable optical stimulators
US10426970B2 (en) 2007-10-31 2019-10-01 The Board Of Trustees Of The Leland Stanford Junior University Implantable optical stimulators
US20090112133A1 (en) * 2007-10-31 2009-04-30 Karl Deisseroth Device and method for non-invasive neuromodulation
US10035027B2 (en) 2007-10-31 2018-07-31 The Board Of Trustees Of The Leland Stanford Junior University Device and method for ultrasonic neuromodulation via stereotactic frame based technique
US20110022129A1 (en) * 2007-11-05 2011-01-27 Prud Homme Robert K Nanoparticles for photodynamic therapy
US9526914B2 (en) 2007-11-06 2016-12-27 Duke University Non-invasive energy upconversion methods and systems
US9526913B2 (en) 2007-11-06 2016-12-27 Duke University Non-invasive energy upconversion methods and systems
US20110220172A1 (en) * 2007-11-06 2011-09-15 Pacific Integrated Energy, Inc. Photo induced enhanced field electron emission collector
US8507785B2 (en) 2007-11-06 2013-08-13 Pacific Integrated Energy, Inc. Photo induced enhanced field electron emission collector
US9302116B2 (en) * 2007-11-06 2016-04-05 Duke University Non-invasive energy upconversion methods and systems for in-situ photobiomodulation
US20110021970A1 (en) * 2007-11-06 2011-01-27 Duke University Non-invasive energy upconversion methods and systems for in-situ photobiomodulation
US8969710B2 (en) 2007-11-06 2015-03-03 Pacific Integrated Energy, Inc. Photon induced enhanced field electron emission collector
US10384071B2 (en) 2007-11-06 2019-08-20 Immunolight, Llc. Non-invasive energy upconversion methods and systems
US10493296B2 (en) 2007-11-06 2019-12-03 Immunolight, Llc Non-invasive energy upconversion methods and systems for in-situ photobiomodulation
US11571587B2 (en) * 2008-03-11 2023-02-07 Immunolight, Llc. Method for treating a disease, disorder, or condition using inhalation to administer an activatable pharmaceutical agent, an energy modulation agent, or both
US10363541B2 (en) 2008-03-11 2019-07-30 Immunolight, Llc. Systems and methods for interior energy-activation from an exterior source
US20100004623A1 (en) * 2008-03-27 2010-01-07 Angiodynamics, Inc. Method for Treatment of Complications Associated with Arteriovenous Grafts and Fistulas Using Electroporation
US9249200B2 (en) 2008-04-23 2016-02-02 The Board Of Trustees Of The Leland Stanford Junior University Expression vector comprising a nucleotide sequence encoding a Volvox carteri light-activated ion channel protein (VChR1) and implantable device thereof
US10350430B2 (en) 2008-04-23 2019-07-16 The Board Of Trustees Of The Leland Stanford Junior University System comprising a nucleotide sequence encoding a volvox carteri light-activated ion channel protein (VCHR1)
US8815582B2 (en) 2008-04-23 2014-08-26 The Board Of Trustees Of The Leland Stanford Junior University Mammalian cell expressing Volvox carteri light-activated ion channel protein (VChR1)
US9394347B2 (en) 2008-04-23 2016-07-19 The Board Of Trustees Of The Leland Stanford Junior University Methods for treating parkinson's disease by optically stimulating target cells
US9878176B2 (en) 2008-04-23 2018-01-30 The Board Of Trustees Of The Leland Stanford Junior University System utilizing Volvox carteri light-activated ion channel protein (VChR1) for optical stimulation of target cells
US10426844B2 (en) 2008-05-20 2019-10-01 University Of Florida Research Foundation, Incorporated Capsid-mutated rAAV vectors and methods of use
US9453215B2 (en) 2008-05-29 2016-09-27 The Board Of Trustees Of The Leland Stanford Junior University Cell line, system and method for optical control of secondary messengers
US8962589B2 (en) 2008-05-29 2015-02-24 The Board Of Trustees Of The Leland Stanford Junior University Cell line, system and method for optical control of secondary messengers
US8729040B2 (en) 2008-05-29 2014-05-20 The Board Of Trustees Of The Leland Stanford Junior University Cell line, system and method for optical control of secondary messengers
US10711242B2 (en) 2008-06-17 2020-07-14 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for controlling cellular development
US20110159562A1 (en) * 2008-06-17 2011-06-30 Karl Deisseroth Apparatus and methods for controlling cellular development
US20110172653A1 (en) * 2008-06-17 2011-07-14 Schneider M Bret Methods, systems and devices for optical stimulation of target cells using an optical transmission element
US8956363B2 (en) 2008-06-17 2015-02-17 The Board Of Trustees Of The Leland Stanford Junior University Methods, systems and devices for optical stimulation of target cells using an optical transmission element
US9084885B2 (en) 2008-06-17 2015-07-21 The Board Of Trustees Of The Leland Stanford Junior University Methods, systems and devices for optical stimulation of target cells using an optical transmission element
US10583309B2 (en) 2008-07-08 2020-03-10 The Board Of Trustees Of The Leland Stanford Junior University Materials and approaches for optical stimulation of the peripheral nervous system
US9308392B2 (en) 2008-07-08 2016-04-12 The Board Of Trustees Of The Leland Stanford Junior University Materials and approaches for optical stimulation of the peripheral nervous system
US20110166632A1 (en) * 2008-07-08 2011-07-07 Delp Scott L Materials and approaches for optical stimulation of the peripheral nervous system
US9101759B2 (en) 2008-07-08 2015-08-11 The Board Of Trustees Of The Leland Stanford Junior University Materials and approaches for optical stimulation of the peripheral nervous system
US8770203B2 (en) 2008-07-14 2014-07-08 Immunolight, Llc. Advanced methods and systems for treating cell proliferation disorders
US10300299B2 (en) 2008-07-14 2019-05-28 Immunolight, Llc Advanced methods and systems for treating cell proliferation disorders
US9833634B2 (en) 2008-07-14 2017-12-05 Immunolight, Llc Advanced methods and systems for treating cell proliferation disorders
US10835756B2 (en) 2008-07-14 2020-11-17 Immunolight, Llc Advanced methods and systems for treating cell proliferation disorders
US20100022892A1 (en) * 2008-07-23 2010-01-28 Snu R&Db Foundation Method and device for neural user interface and brain activity measuring device for the same
US10064912B2 (en) 2008-11-14 2018-09-04 The Board Of Trustees Of The Leland Stanford Junior University Optically-based stimulation of target cells and modifications thereto
US9458208B2 (en) 2008-11-14 2016-10-04 The Board Of Trustees Of The Leland Stanford Junior University Optically-based stimulation of target cells and modifications thereto
US9309296B2 (en) 2008-11-14 2016-04-12 The Board Of Trustees Of The Leland Stanford Junior University Optically-based stimulation of target cells and modifications thereto
US10071132B2 (en) 2008-11-14 2018-09-11 The Board Of Trustees Of The Leland Stanford Junior University Optically-based stimulation of target cells and modifications thereto
US20100152715A1 (en) * 2008-12-14 2010-06-17 Pattanam Srinivasan Method for Deep Tissue Laser Treatments Using Low Intensity Laser Therapy Causing Selective Destruction of Nociceptive Nerves
US9693825B2 (en) 2008-12-14 2017-07-04 C Laser, Inc. Fiber embedded hollow needle for percutaneous delivery of laser energy
US9149647B2 (en) * 2008-12-14 2015-10-06 C Laser, Inc. Method for deep tissue laser treatments using low intensity laser therapy causing selective destruction of Nociceptive nerves
US11324965B2 (en) 2009-04-21 2022-05-10 Immunoloight, Llc. Non-invasive energy upconversion methods and systems
US11383098B2 (en) 2009-04-21 2022-07-12 Immunolight, Llc Non-invasive energy upconversion methods and systems for in-situ photobiomodulation
US11707629B2 (en) 2009-05-28 2023-07-25 Angiodynamics, Inc. System and method for synchronizing energy delivery to the cardiac rhythm
US9895189B2 (en) 2009-06-19 2018-02-20 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
US20110137210A1 (en) * 2009-12-08 2011-06-09 Johnson Marie A Systems and methods for detecting cardiovascular disease
US20110166560A1 (en) * 2010-01-07 2011-07-07 Solar System Beauty Corporation Skin care laser device
US9782221B2 (en) * 2010-02-21 2017-10-10 C Laser, Inc. Treatment using low intensity laser therapy
US20160015997A1 (en) * 2010-02-21 2016-01-21 C Laser, Inc. Treatment Using Low Intensity Laser Therapy
US10206742B2 (en) 2010-02-21 2019-02-19 C Laser, Inc. Fiber embedded hollow spikes for percutaneous delivery of laser energy
US9265576B2 (en) 2010-02-21 2016-02-23 C Laser, Inc. Laser generator for medical treatment
US9249234B2 (en) 2010-03-17 2016-02-02 The Board Of Trustees Of The Leland Stanford Junior University Light-sensitive ion-passing molecules
US9604073B2 (en) 2010-03-17 2017-03-28 The Board Of Trustees Of The Leland Stanford Junior University Light-sensitive ion-passing molecules
US9359449B2 (en) 2010-03-17 2016-06-07 The Board Of Trustees Of The Leland Stanford Junior University Light-sensitive ion-passing molecules
US9079940B2 (en) 2010-03-17 2015-07-14 The Board Of Trustees Of The Leland Stanford Junior University Light-sensitive ion-passing molecules
US11771763B2 (en) 2010-04-05 2023-10-03 Eos Neuroscience, Inc. Methods and compositions for decreasing chronic pain
US9348078B2 (en) 2010-06-08 2016-05-24 Pacific Integrated Energy, Inc. Optical antennas with enhanced fields and electron emission
WO2011163646A3 (en) * 2010-06-25 2012-04-19 Marek Malecki Methods for detection, diagnosis and selective eradication of neoplasms and circulating tumor cells using multidomain biotags
WO2011163646A2 (en) * 2010-06-25 2011-12-29 Marek Malecki Methods for detection, diagnosis and selective eradication of neoplasms and circulating tumor cells using multidomain biotags
US8192429B2 (en) 2010-06-29 2012-06-05 Theravant, Inc. Abnormality eradication through resonance
US9421260B2 (en) 2010-08-27 2016-08-23 Sienna Biopharmaceuticals, Inc. Thermal treatment of acne with nanoparticles with coatings that facilitate selective removal from the skin surface
US11826087B2 (en) 2010-08-27 2023-11-28 Coronado Aesthetics, Llc Compositions and methods for thermal skin treatment with metal nanoparticles
US9446126B2 (en) 2010-08-27 2016-09-20 Sienna Biopharmaceuticals, Inc. Thermal treatment of acne with coated metal nanoparticles
US9439965B2 (en) 2010-08-27 2016-09-13 Sienna Biopharmaceuticals, Inc. Thermal treatment of the skin surface with metal nanoparticles in surfactant containing solutions
US9061056B2 (en) 2010-08-27 2015-06-23 Sienna Labs, Inc. Compositions and methods for targeted thermomodulation
US9439964B2 (en) 2010-08-27 2016-09-13 Sienna Biopharmaceuticals, Inc. Thermal treatment of the skin surface with coated metal nanoparticles
US8906418B1 (en) 2010-08-27 2014-12-09 Sienna Labs, Inc. Thermal treatment of a pilosebaceous unit with nanoparticles with coatings that facilitate selective removal from the skin surface
EP3210591B1 (en) 2010-08-27 2019-01-02 Sienna Biopharmaceuticals, Inc. Compositions and methods for targeted thermomodulation
US8895071B1 (en) 2010-08-27 2014-11-25 Sienna Labs, Inc. Thermal treatment of a pilosebaceous unit with coated metal nanoparticles
EP2608762B1 (en) 2010-08-27 2017-07-19 Sienna Biopharmaceuticals, Inc. Compositions and methods for targeted thermomodulation
US11419937B2 (en) 2010-08-27 2022-08-23 Coronado Aesthetics, Llc Delivery of nanoparticles
US9433677B2 (en) 2010-08-27 2016-09-06 Sienna Biopharmaceuticals, Inc. Thermal treatment of a pilosebaceous unit with metal nanoparticles in surfactant containing solutions
US8834933B2 (en) 2010-08-27 2014-09-16 Sienna Labs, Inc. Thermal treatment of acne with nanoparticles
EP2608762B2 (en) † 2010-08-27 2020-05-13 Sienna Biopharmaceuticals, Inc. Compositions and methods for targeted thermomodulation
US10537640B2 (en) 2010-08-27 2020-01-21 Sienna Biopharmaceuticals, Inc. Ultrasound delivery of nanoparticles
US9433676B2 (en) 2010-08-27 2016-09-06 Sienna Biopharmaceuticals, Inc. Hair removal with nanoparticles with coatings that facilitate selective removal from the skin surface
US9433678B2 (en) 2010-08-27 2016-09-06 Sienna Biopharmaceuticals, Inc. Thermal treatment of acne with metal nanoparticles in surfactant containing solutions
US8821940B2 (en) 2010-08-27 2014-09-02 Sienna Labs, Inc. Thermal treatment of the skin surface with nanoparticles
US9427467B2 (en) 2010-08-27 2016-08-30 Sienna Biopharmaceuticals, Inc. Hair removal with metal nanoparticles in surfactant containing solutions
US9421261B2 (en) 2010-08-27 2016-08-23 Sienna Biopharmaceuticals, Inc. Thermal treatment of the skin surface with nanoparticles with coatings that facilitate selective removal from the skin surface
US9572880B2 (en) 2010-08-27 2017-02-21 Sienna Biopharmaceuticals, Inc. Ultrasound delivery of nanoparticles
US8821941B2 (en) 2010-08-27 2014-09-02 Sienna Labs, Inc. Hair removal with nanoparticles
US9421259B2 (en) 2010-08-27 2016-08-23 Sienna Biopharmaceuticals, Inc. Hair removal with coated metal nanoparticles
US8802154B2 (en) 2010-08-27 2014-08-12 Sienna Labs, Inc. Thermal treatment of a pilosebaceous unit with nanoparticles
EP3222266B1 (en) 2010-08-27 2018-04-18 Sienna Biopharmaceuticals, Inc. Compositions and methods for targeted thermomodulation
EP3006014A1 (en) * 2010-09-14 2016-04-13 L'oreal Cosmetic composition comprising a dyestuff, said dyestuff and cosmetic treatment process
US11931096B2 (en) 2010-10-13 2024-03-19 Angiodynamics, Inc. System and method for electrically ablating tissue of a patient
US9968652B2 (en) 2010-11-05 2018-05-15 The Board Of Trustees Of The Leland Stanford Junior University Optically-controlled CNS dysfunction
US9340589B2 (en) 2010-11-05 2016-05-17 The Board Of Trustees Of The Leland Stanford Junior University Light-activated chimeric opsins and methods of using the same
US9850290B2 (en) 2010-11-05 2017-12-26 The Board Of Trustees Of The Leland Stanford Junior University Light-activated chimeric opsins and methods of using the same
US10086012B2 (en) 2010-11-05 2018-10-02 The Board Of Trustees Of The Leland Stanford Junior University Control and characterization of memory function
US10196431B2 (en) 2010-11-05 2019-02-05 The Board Of Trustees Of The Leland Stanford Junior University Light-activated chimeric opsins and methods of using the same
US10252076B2 (en) 2010-11-05 2019-04-09 The Board Of Trustees Of The Leland Stanford Junior University Upconversion of light for use in optogenetic methods
US9992981B2 (en) 2010-11-05 2018-06-12 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of reward-related behaviors
US10568307B2 (en) 2010-11-05 2020-02-25 The Board Of Trustees Of The Leland Stanford Junior University Stabilized step function opsin proteins and methods of using the same
US9522288B2 (en) 2010-11-05 2016-12-20 The Board Of Trustees Of The Leland Stanford Junior University Upconversion of light for use in optogenetic methods
US9421258B2 (en) 2010-11-05 2016-08-23 The Board Of Trustees Of The Leland Stanford Junior University Optically controlled CNS dysfunction
US8932562B2 (en) 2010-11-05 2015-01-13 The Board Of Trustees Of The Leland Stanford Junior University Optically controlled CNS dysfunction
US10914803B2 (en) 2010-11-22 2021-02-09 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
US8834546B2 (en) 2010-11-22 2014-09-16 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
US8696722B2 (en) * 2010-11-22 2014-04-15 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
US10018695B2 (en) 2010-11-22 2018-07-10 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
US9271674B2 (en) 2010-11-22 2016-03-01 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
US20120165904A1 (en) * 2010-11-22 2012-06-28 Jin Hyung Lee Optogenetic magnetic resonance imaging
US10371776B2 (en) 2010-11-22 2019-08-06 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
US9615789B2 (en) 2010-11-22 2017-04-11 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic magnetic resonance imaging
US10441808B2 (en) * 2010-12-29 2019-10-15 Ethicon Endo-Surgery, Inc. Methods and devices for activating brown adipose tissue with light
US20140088487A1 (en) * 2010-12-29 2014-03-27 Ethicon Endo-Surgery, Inc. Methods and devices for activating brown adipose tissue with light
WO2012102816A1 (en) * 2011-01-24 2012-08-02 Actium BioSystems, LLC System for correlating energy field characteristics with target particle characteristics in a living organism
US8757166B2 (en) 2011-01-24 2014-06-24 Actium BioSystems, LLC System for defining energy field characteristics to illuminate nano-particles used to treat invasive agents
US10124186B2 (en) 2011-01-24 2018-11-13 Endomagnetics Limited System for automatically amending energy field characteristics in the application of an energy field to a living organism for treatment of invasive agents
US8968171B2 (en) 2011-01-24 2015-03-03 Endomagnetics Limited System for correlating energy field characteristics with target particle characteristics in the application of an energy field to a living organism for imaging and treatment of invasive agents
US9180307B2 (en) * 2011-03-15 2015-11-10 St. Jude Medical, Atrial Fibrillation Division, Inc. Method of reducing the occurrence of arrhythmias via photobiomodulation and apparatus for same
US20120239121A1 (en) * 2011-03-15 2012-09-20 Jaerverud Karin Method of reducing the occurrence of arrhythmias via photobiomodulation and apparatus for same
US9440091B2 (en) 2011-03-15 2016-09-13 St. Jude Medical, Atrial Fibrillation Division, Inc. Method of reducing the occurrence of arrhythmias via photobiomodulation and apparatus for same
US20150320599A1 (en) * 2011-06-24 2015-11-12 The Regents Of The University Of California Nonlinear optical photodynamic therapy (nlo-pdt) of the cornea
US10292865B2 (en) * 2011-06-24 2019-05-21 The Regents Of The University Of California Nonlinear optical photodynamic therapy (NLO-PDT) of the cornea
US9687668B2 (en) 2011-08-26 2017-06-27 Endomagnetics Limited Treatment of cancer in body cavities and parts that are cavity-like
US9682247B2 (en) 2011-08-26 2017-06-20 Endomagnetics Limited Apparatus for the generation of an energy field for the treatment of cancer in body cavities and parts that are cavity-like
US9757196B2 (en) 2011-09-28 2017-09-12 Angiodynamics, Inc. Multiple treatment zone ablation probe
US11779395B2 (en) 2011-09-28 2023-10-10 Angiodynamics, Inc. Multiple treatment zone ablation probe
US9267889B1 (en) * 2011-10-12 2016-02-23 Stc.Unm High efficiency light absorbing and light emitting nanostructures
US20140356897A1 (en) * 2011-12-08 2014-12-04 The Washington University In vivo label-free histology by photoacoustic microscopy of cell nuclei
US9969783B2 (en) 2011-12-16 2018-05-15 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
US9840541B2 (en) 2011-12-16 2017-12-12 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
US10538560B2 (en) 2011-12-16 2020-01-21 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
US10087223B2 (en) 2011-12-16 2018-10-02 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
US9365628B2 (en) 2011-12-16 2016-06-14 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
US9505817B2 (en) 2011-12-16 2016-11-29 The Board Of Trustees Of The Leland Stanford Junior University Opsin polypeptides and methods of use thereof
US11103723B2 (en) 2012-02-21 2021-08-31 The Board Of Trustees Of The Leland Stanford Junior University Methods for treating neurogenic disorders of the pelvic floor
US9764305B2 (en) * 2012-04-06 2017-09-19 The Regents Of The University Of California Geometry enhancement of nanoscale energy deposition by X-rays
US20150083579A1 (en) * 2012-04-06 2015-03-26 The Regents Of The University Of California Geometry enhancement of nanoscale energy deposition by x-rays
US10322194B2 (en) 2012-08-31 2019-06-18 Sloan-Kettering Institute For Cancer Research Particles, methods and uses thereof
US9410007B2 (en) 2012-09-27 2016-08-09 Rhodia Operations Process for making silver nanostructures and copolymer useful in such process
US11583553B2 (en) 2012-10-11 2023-02-21 Nanocomposix, Llc Silver nanoplate compositions and methods
US9526745B2 (en) 2012-10-11 2016-12-27 Nanocomposix, Inc. Silver nanoplate compositions and methods
US9249334B2 (en) 2012-10-11 2016-02-02 Nanocomposix, Inc. Silver nanoplate compositions and methods
US10688126B2 (en) 2012-10-11 2020-06-23 Nanocomposix, Inc. Silver nanoplate compositions and methods
US9212294B2 (en) 2012-10-11 2015-12-15 Nanocomposix, Inc. Silver nanoplate compositions and methods
US20160356900A1 (en) * 2012-10-16 2016-12-08 Nanoptics, Incorporated Method and apparatus for neutron detection utilizing pulse height discrimination and pulse shape discrimination
US9772411B2 (en) * 2012-10-16 2017-09-26 Nanoptics, Incorporated Method and apparatus for neutron detection utilizing pulse height discrimination and pulse shape discrimination
US10105456B2 (en) 2012-12-19 2018-10-23 Sloan-Kettering Institute For Cancer Research Multimodal particles, methods and uses thereof
US9888956B2 (en) 2013-01-22 2018-02-13 Angiodynamics, Inc. Integrated pump and generator device and method of use
US20140350534A1 (en) * 2013-02-20 2014-11-27 Sloan-Kettering Institute For Cancer Research Raman based ablation/resection systems and methods
US10888227B2 (en) 2013-02-20 2021-01-12 Memorial Sloan Kettering Cancer Center Raman-triggered ablation/resection systems and methods
US20140254752A1 (en) * 2013-03-07 2014-09-11 Farida A. Selim Luminescence based spectrometers
US9261469B2 (en) * 2013-03-07 2016-02-16 Farida A. Selim Luminescence based spectrometers
US10974064B2 (en) 2013-03-15 2021-04-13 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of behavioral state
US11678682B2 (en) 2013-03-15 2023-06-20 Immunolight, Llc Phosphor composition having selected surface coatings
US11278042B2 (en) 2013-03-15 2022-03-22 Immunolight, Llc Phosphor composition having selected surface coatings
US10874123B2 (en) * 2013-03-15 2020-12-29 Immunolight, Llc Phosphor composition having selected surface coatings
US20200196639A1 (en) * 2013-03-15 2020-06-25 Immunolight, Llc. Phosphor composition having selected surface coatings
US9636380B2 (en) 2013-03-15 2017-05-02 The Board Of Trustees Of The Leland Stanford Junior University Optogenetic control of inputs to the ventral tegmental area
US10220092B2 (en) 2013-04-29 2019-03-05 The Board Of Trustees Of The Leland Stanford Junior University Devices, systems and methods for optogenetic modulation of action potentials in target cells
US11957405B2 (en) 2013-06-13 2024-04-16 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
US10307609B2 (en) 2013-08-14 2019-06-04 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for controlling pain
US9588327B2 (en) * 2013-12-12 2017-03-07 The Board Of Trustees Of The University Of Illinois Nanoindenter multimodal microscope objective for mechanobiology
US20150168239A1 (en) * 2013-12-12 2015-06-18 The Board Of Trustees Of The University Of Illinois Nanoindenter Multimodal Microscope Objective for Mechanobiology
US10912947B2 (en) 2014-03-04 2021-02-09 Memorial Sloan Kettering Cancer Center Systems and methods for treatment of disease via application of mechanical force by controlled rotation of nanoparticles inside cells
US11345833B2 (en) * 2014-03-18 2022-05-31 Immunolight, Llc Adhesive bonding composition and method of use
US20150265397A1 (en) * 2014-03-21 2015-09-24 Carl Zeiss Meditec Ag Method for transplanting a part of the cornea and a surgical microscope therefor
US11865359B2 (en) 2014-04-22 2024-01-09 Immunolight, Llc. Tumor imaging with x-rays and other high energy sources using as contrast agents photon-emitting phosphors having therapeutic properties
EP3134122A4 (en) * 2014-04-22 2017-12-20 Immunolight, Llc. Tumor imaging using photon-emitting phosphors having therapeutic properties
US10688202B2 (en) 2014-07-28 2020-06-23 Memorial Sloan-Kettering Cancer Center Metal(loid) chalcogen nanoparticles as universal binders for medical isotopes
US11534622B2 (en) * 2014-08-18 2022-12-27 Immunolight, Llc Non-invasive systems and methods for selective activation of photoreactive responses
WO2016028680A1 (en) * 2014-08-18 2016-02-25 Immunolight, Llc. Non-invasive systems and methods for selective activation of photoreactive responses
US11007207B2 (en) * 2014-08-29 2021-05-18 University Of South Carolina Preparations of gold/mesoporous silica hybrid nanoparticle and applications
US20160067354A1 (en) * 2014-08-29 2016-03-10 University Of South Carolina Preparations of gold/mesoporous silica hybrid nanoparitcle and applications
US10881550B2 (en) 2014-09-09 2021-01-05 LumiThera, Inc. Multi-wavelength phototherapy systems and methods for the treatment of damaged or diseased tissue
US10596037B2 (en) 2014-09-09 2020-03-24 LumiThera, Inc. Devices and methods for non-invasive multi-wavelength photobiomodulation for ocular treatments
US10219944B2 (en) 2014-09-09 2019-03-05 LumiThera, Inc. Devices and methods for non-invasive multi-wavelength photobiomodulation for ocular treatments
AU2016206832B2 (en) * 2015-01-14 2021-04-01 Immunolight, Llc. Non-invasive systems and methods for treatment of a host carrying a virus with photoactivatable drugs
EP3244928A4 (en) * 2015-01-14 2018-08-01 Immunolight, Llc. Non-invasive systems and methods for treatment of a host carrying a virus with photoactivatable drugs
US10472340B2 (en) 2015-02-17 2019-11-12 Arizona Board Of Regents On Behalf Of Arizona State University Substituted phenothiazines as mitochondrial agents
US10364227B2 (en) 2015-02-17 2019-07-30 Arizona Board Of Regents On Behalf Of Arizona State University Therapeutic compounds
US11034662B2 (en) 2015-02-17 2021-06-15 Arizona Board Of Regents On Behalf Of Arizona State University Substituted phenothiazines as mitochondrial agents
US10568516B2 (en) 2015-06-22 2020-02-25 The Board Of Trustees Of The Leland Stanford Junior University Methods and devices for imaging and/or optogenetic control of light-responsive neurons
US10919089B2 (en) 2015-07-01 2021-02-16 Memorial Sloan Kettering Cancer Center Anisotropic particles, methods and uses thereof
US11642547B2 (en) * 2015-07-08 2023-05-09 Wave Force Electronics Inc. Bioresonance frequency emitting device, system, and method
US20170007847A1 (en) * 2015-07-08 2017-01-12 Wave Force Electronics Inc. Bioresonance frequency emitting device, system, and method
EP3365017A4 (en) * 2015-10-19 2019-04-17 Immunolight, LLC X-PACT ACTIVATED ANTI-CANCER TREATMENT BASED ON X-RAY-ACTIVATED PSORALENE
US11305131B2 (en) 2015-10-19 2022-04-19 Immunolight, Llc X-ray psoralen activated cancer therapy (X-PACT)
WO2017132639A1 (en) * 2016-01-30 2017-08-03 The Board Of Trustees Of The Leland Stanford Junior University Light-activated anchoring of therapeutic factors to tissues
KR20190014504A (ko) * 2016-04-25 2019-02-12 이뮤노라이트, 엘엘씨 삽입 디바이스들 및 매질 내부에 방출 광의 생산을 위한 시스템들 및 이들의 사용 방법들
WO2017189506A1 (en) * 2016-04-25 2017-11-02 Immunolight, Llc Insertion devices and systems for production of emitted light internal to a medium and methods for their use
US11077316B2 (en) 2016-04-25 2021-08-03 Immunolight, Llc Insertion devices and systems for production of emitted light internal to a medium and methods for their use
KR102465104B1 (ko) * 2016-04-25 2022-11-10 이뮤노라이트, 엘엘씨 삽입 디바이스들 및 매질 내부에 방출 광의 생산을 위한 시스템들 및 이들의 사용 방법들
CN105919592A (zh) * 2016-05-20 2016-09-07 北京普康大健康管理服务中心 一种schl量子生物能量全息分析系统和分析方法
WO2017218537A1 (en) * 2016-06-15 2017-12-21 Arizona Board Of Regents On Behalf Of Arizona State University Prodrug and profluorescent compounds for selective mitochondrial imaging and therapeutic targeting
US11203583B2 (en) 2016-06-15 2021-12-21 Arizona Board Of Regents On Behalf Of Arizona State University Prodrug and profluorescent compounds for selective mitochondrial imaging and therapeutic targeting
US10604501B2 (en) 2016-06-15 2020-03-31 Arizona Board Of Regents On Behalf Of Arizona State University Prodrug and profluorescent compounds for selective mitochondrial imaging and therapeutic targeting
US20190299021A1 (en) * 2016-08-05 2019-10-03 Nagasaki Method & Co., Ltd. HEAD PHOTIC STIMULATION DEVICE, HEAD PHOTIC STIMULATION METHOD, AND PROGRAM [As Amended]
US11331511B2 (en) * 2016-08-05 2022-05-17 Nagasaki Method & Co., Ltd. Head photic stimulation device, head photic stimulation method, and program
US11390605B2 (en) 2016-08-25 2022-07-19 Arizona Board Of Regents On Behalf Of Arizona State University Substituted pyrimidine compounds as multifunctional radical quenchers and their uses
US11844605B2 (en) 2016-11-10 2023-12-19 The Research Foundation For Suny System, method and biomarkers for airway obstruction
US11723710B2 (en) 2016-11-17 2023-08-15 Angiodynamics, Inc. Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode
US20180229299A1 (en) * 2017-02-15 2018-08-16 The Board Of Trustees Of The University Of Arkansas Copper-silica core-shell nanoparticles and methods
US11294165B2 (en) 2017-03-30 2022-04-05 The Board Of Trustees Of The Leland Stanford Junior University Modular, electro-optical device for increasing the imaging field of view using time-sequential capture
US11331019B2 (en) 2017-08-07 2022-05-17 The Research Foundation For The State University Of New York Nanoparticle sensor having a nanofibrous membrane scaffold
US11723579B2 (en) 2017-09-19 2023-08-15 Neuroenhancement Lab, LLC Method and apparatus for neuroenhancement
US11484731B2 (en) 2017-11-09 2022-11-01 International Business Machines Corporation Cognitive optogenetics probe and analysis
US11717686B2 (en) 2017-12-04 2023-08-08 Neuroenhancement Lab, LLC Method and apparatus for neuroenhancement to facilitate learning and performance
US11273283B2 (en) 2017-12-31 2022-03-15 Neuroenhancement Lab, LLC Method and apparatus for neuroenhancement to enhance emotional response
US11318277B2 (en) 2017-12-31 2022-05-03 Neuroenhancement Lab, LLC Method and apparatus for neuroenhancement to enhance emotional response
US11478603B2 (en) 2017-12-31 2022-10-25 Neuroenhancement Lab, LLC Method and apparatus for neuroenhancement to enhance emotional response
US11364361B2 (en) 2018-04-20 2022-06-21 Neuroenhancement Lab, LLC System and method for inducing sleep by transplanting mental states
US11452839B2 (en) 2018-09-14 2022-09-27 Neuroenhancement Lab, LLC System and method of improving sleep
US10821296B2 (en) * 2019-01-15 2020-11-03 Nadia Ansari Systems and methods for the automated delivery of photobiomodulation therapy to a patient
US20200222713A1 (en) * 2019-01-15 2020-07-16 Nadia Ansari Systems and Methods for the Automated Delivery of Photobiomodulation Therapy to a Patient
WO2020180425A1 (en) * 2019-03-04 2020-09-10 Immunolight, Llc. Energy augment structures for use with energy emitters and collectors
WO2020180426A1 (en) * 2019-03-04 2020-09-10 Immunolight, Llc. Energy augmentation structures, energy emitters or energy collectors containing the same, and their use in methods and systems for treating cell proliferation disorders
US11964167B2 (en) 2019-03-04 2024-04-23 Immunolight, Llc Energy augmentation structures for use with energy emitters and collectors
US11786749B2 (en) * 2020-05-05 2023-10-17 Lumeda Inc. Time mulitplexed dosimetry system and method
US20230173299A1 (en) * 2020-05-05 2023-06-08 Lumeda Inc. Time mulitplexed dosimetry system and method
US11992697B2 (en) 2022-03-21 2024-05-28 Immunolight, Llc X-ray psoralen activated cancer therapy (X-PACT)

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