US20070221684A1 - Device for Receiving and Dispensing Liquids - Google Patents

Device for Receiving and Dispensing Liquids Download PDF

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Publication number
US20070221684A1
US20070221684A1 US11/578,826 US57882605A US2007221684A1 US 20070221684 A1 US20070221684 A1 US 20070221684A1 US 57882605 A US57882605 A US 57882605A US 2007221684 A1 US2007221684 A1 US 2007221684A1
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Prior art keywords
plate
stop
piston
cylinder
liquid
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Abandoned
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US11/578,826
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English (en)
Inventor
Bernd Steinbrenner
Roger Steinbrenner
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Individual
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Individual
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
    • B67D—DISPENSING, DELIVERING OR TRANSFERRING LIQUIDS, NOT OTHERWISE PROVIDED FOR
    • B67D99/00—Subject matter not provided for in other groups of this subclass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Definitions

  • the invention concerns a device for receiving and dispensing liquids, with a plurality of piston-cylinder units which are disposed on a piston plate and a cylinder plate, respectively, wherein the liquid is received and dispensed by displacing the pistons in the cylinders by changing the distance between the plates using a drive mechanism and a plate guidance, perpendicularly to the plate planes, wherein the volume can be determined by an upper and a lower stop, at least one of which can be adjusted using at least one adjusting means.
  • a spindle drive of this type having the required rapid and precise simultaneous spindle speed, is inappropriate for a pipetting system which is operated manually or with a simple drive, since either the mechanical devices or the control and drive are excessively complex or expensive.
  • U.S. Pat. No. 5,540,889 discloses a simple mechanical device having the features of the above-mentioned type.
  • the piston plate is moved relative to the cylinder plate by a manual drive rod mounted to the center of the piston plate.
  • An adjustable stop for presetting the pipetting volume is provided on the manual drive rod.
  • the parallelism between the piston plate and the cylinder plate is thereby achieved by the manual drive rod guidance and optionally also by the piston guidance in the cylinders.
  • This principle corresponds to the conventional multi-channel pipettes (the above-mentioned 8 or 12-channel pipettes).
  • This object is achieved in accordance with the invention, while guaranteeing identical receiving and dispensing volumes of the piston-cylinder units with low demands with regard to surface parallelism of the plate planes during motion in the plate guidance, by designing and disposing the stops in such a manner that the stops ensure that the plates are parallel in the upper and lower stop positions.
  • uniform motion of all pistons i.e. permanent parallelism between piston and cylinder plates during the entire pipetting process, is not required in order to pipette exact volumes.
  • all piston-cylinder units are at the same position at only two times: at the lower and upper end positions. This is ensured by the stops.
  • these two positions which correspond to the start and end of liquid reception or, vice versa, liquid dispensing, are exactly correct, it is irrelevant for the exactness of the volume whether the plates are exactly parallel inbetween, since any irregularity is compensated for at the latest when the respective stop is reached. This applies both for receiving and dispensing liquid.
  • the precision therefore does not depend on the pipetting drive.
  • the invention thereby meets two conditions for precise and reproducible pipetting.
  • the plates are aligned exactly in parallel by the stops at the initial and end positions which are decisive for determining the volumes.
  • All piston-cylinder units have thereby moved through the same distance, which does not only exactly define the pipetting volume but also ensures that it is identical for each individual piston-cylinder unit of the system.
  • Each unit pipettes the same volume, and all volumes can be simultaneously adjusted with high accuracy. Moreover, the operating forces are also within a practical range.
  • the above-presented device represents the first inexpensive device for performing a large number of parallel pipetting processes for high throughput work. No great investment is required, and the running costs and the overall weight of the device are substantially less compared to the above-mentioned automats. This is beneficial, in particular, for small companies, universities or institutes and companies in developing countries.
  • the inventive device closes the gap between multi-channel pipette and fully automated systems. It permits parallel reception or dispensing of many, preferably 96 liquid samples. The volume can be freely selected and exactly adjusted.
  • the pipetting process itself is simple and easy to learn and corresponds largely to conventional manual pipetting which has been practiced for decades.
  • the up and down motion between the stops need not be controlled.
  • An electronic control or electric drive is therefore not necessary, although a very simple means of this type could nevertheless be implemented.
  • the stops can be adjusted by a simple adjusting motion using a display means.
  • the system can therefore be operated without extensive programming. Even unskilled staff can handle operation thereof without requiring time-consuming and expensive training.
  • faultless operation can be visually controlled at any time.
  • the system can be quickly and flexibly adjusted to the respective requirements and can be used even when the space is limited, e.g. on sterile workbenches, due to its small dimensions.
  • the stops may be formed in different ways. They must only ensure that the plates, i.e. the piston plate and the cylinder plate, are parallel relative to each other in the upper and lower stop positions.
  • the cylinder plate When e.g. the piston plate is moved, the cylinder plate may be used as lower stop or bear such stops. In this case, only one upper stop must be mounted.
  • This stop may also be a flat plate or consist of two linear stops.
  • each of the upper and lower stops has at least three stop points which abut the outer area of the moved plate. A minimum of three points is required to define the exact position of a surface. Pipetting processes are generally performed using working plates having an orthogonal grid and therefore a rectangular shape. For this reason, the piston plate and the cylinder plate will also have a rectangular shape. In this case, four stop points at the plate corners would be suitable, wherein, as mentioned above, three are also clearly possible.
  • the stops are preferably provided with means for adjusting the parallelism of the stop positions, which provides both exact initial setting and exact further readjustment.
  • the invention proposes to design the adjusting means for adjusting the volume such that the stop points of the adjustable stop can simultaneously be adjusted in height.
  • the volume can be adjusted through one single operation, wherein the parallelism of the plates in the end positions and thereby exact volumes of all piston-cylinder units are ensured, which may be achieved in different ways.
  • the stop points may e.g. be adjusted by stepping motors. This can be realized with a very simple control means with one single display.
  • the stop points may e.g. be adjusted by threaded spindles or by adjustment nuts which can be screwed on rigid threaded spindles, which are simultaneously moved by an adjusting means via a toothed belt and toothed wheels.
  • This adjustment can be performed without the plate abutting against and loading the stop.
  • an abutting plate e.g. the piston plate
  • the latter should suitably be previously moved away to ensure smooth running and reduce the required force.
  • measures may be taken to ensure this, such as blocking of the adjusting means or automatically moving away the plate when the adjusting means for the stop are initially actuated.
  • This adjusting means suitably comprises a display means which displays the adjusted volumes.
  • the adjusting mechanism is released from all force-related loads, in particular, those of the drive required for the pipetting process, thereby obtaining high accuracy with little expense.
  • the device is advantageously used when a considerably higher number of piston-cylinder units are required than for a 8- or 12-channel pipette, which are designed in a grid-like arrangement, e.g. with 96 units. For this reason, disposing the device on a working plate having the same number of working positions is problematic. It is therefore proposed to dispose the piston plate, the cylinder plate, the stops and the drive in a pipetting head, which is suspended on a stand using a vertical guidance. It is also advantageous for the stand to have means for positioning the pipette head towards a working plate for pipetting in order to align the grid arrangement of the piston-cylinder units with the grid arrangement of the working positions on the working plate. This particularly accelerates working without excessively increasing the skill requirements.
  • Processing with several working plates is often necessary, e.g. to receive a liquid from a reservoir and dispense it to a working plate for processing. It is also feasible to repeatedly receive and dispense liquids to accelerate processing without having to repeatedly exchange the working plates.
  • Liquid receptacles e.g. pipette tips, must often be accommodated in a grid-like arrangement (see below). A liquid is subsequently received, which is then dispensed and the liquid receptacles are finally removed. This would require an arrangement of four working plates.
  • further positioning means for working plates are provided, which can optionally be aligned with the grid arrangement of the piston-cylinder units.
  • the stand has a horizontal guidance for the pipetting head and several positioning means for working plates disposed next to each other below the horizontal guidance, wherein positioning aids are provided for allocating the grids to the horizontal guidance.
  • positioning aids are provided for allocating the grids to the horizontal guidance.
  • Another possibility is to provide several positioning means for working plates, which can be displaced to positions defined by positioning aids, using at least one horizontal guidance therefor, e.g. displaceable trays, such that the working plates located there can be associated with the grid of the piston-cylinder units.
  • the positioning aids may each be end stops of the horizontal guidances.
  • the above-mentioned four, frequently required working plates can be positioned (see FIG. 2 ).
  • Other positions may clearly also be designed by providing locking positions between the stops.
  • At least one lower stop is preferably provided for the vertical motion of the pipetting head. It adjusts a stop position for a vertical downward motion, in which the desired pipetting process can take place, since the correct height for receiving or dispensing liquid is predetermined relative to the respective working plates. For this reason, the at least one lower stop can suitably adjust the immersion depth into the liquid receptacles of the working plates. If e.g. several working plates are in positioning means below the horizontal guidance for the pipetting head, one stop may be provided for each positioning means, to adjust the correct height above the respective working plates.
  • the drive mechanism for the piston-cylinder units can advantageously be operated by a manual lever with transmission.
  • This may e.g. be a corresponding lever transmission or toothed wheel or toothed rack transmission or a combination of both.
  • the moved plate advantageously is the piston plate, such that the drive is advantageously associated with the piston plate.
  • the drive moves the piston plate into its lower stop position and restoring springs return it into the upper stop position. This particularly facilitates operation and prevents the piston plate from resting in an undefined position.
  • the pistons may be sealed with respect to the cylinders. This is advantageous, in that the piston and cylinders need not be accurately adjusted, with sealing being ensured, and a certain tolerance of parallelism of the plates during adjustment is possible without causing jamming.
  • the seals may be designed as sealing rings disposed on the pistons.
  • the cylinder plate may also be divided in a horizontal direction, with elastic sealing material being disposed therein, such that the cylinder diameters can be adjusted at the sealing locations through means for compressing the plate parts, wherein the pistons move in the area of the sealing locations.
  • the sealing material may e.g. be a perforated plate of elastic material or each individual cylinder may have an associated annular elastic sealing element. This design is advantageous in that abutment between the seals and the pistons can be set and readjusted.
  • a certain force is required to receive a large number of such liquid receptacles or pipette tips, e.g. the whole grid, at once.
  • Force transmission is therefore proposed which presses the pipetting head for receiving the liquid receptacle, in particular the pipette tips, against a liquid receptacle support, in particular pipette tip support, located in a positioning means, or vice versa.
  • the force transmission may e.g. be a translation lever which acts between the stand and pipetting head, forcing the latter towards the liquid receptacle support, in particular pipette tip support. Due to this force transmission, the pipetting drive need not be used for this purpose, and it need not be designed to withstand such high forces.
  • a liquid receptacle or pipette tip wiper is provided, which may be a perforated plate operated by a lever, and disposed below the cylinder plate for vertical displacement.
  • the holes must thereby be larger than the cylinder receiving means, but smaller than those of the liquid receptacles or pipette tips, to sweep them from the receiving means.
  • the lower stop is designed as a latch which defines the lift which is decisive to determine the volumes, by parallel orientation of the plate. The plate can then be moved further by overcoming the retention force or disengaging the latch, to blow out any residual liquid.
  • the received liquid can be dosed by disposing at least one latch-like intermediate stop between the upper and lower stops which also aligns the plates in parallel and can be overcome with increased force or through release.
  • At least one latch-like intermediate stop have the purpose that the volumes of liquid defined after one single liquid reception are subsequently dispensed to two or more working plates. This simplifies processing in that several working plates are provided with the same liquid.
  • This at least one latch-like intermediate stop should clearly also be adjustable such that the volumes can also be exactly determined relative to the sequentially dispensed liquid.
  • FIG. 1 shows the functional structure of the inventive device
  • FIG. 2 shows the arrangement of the inventive device on a stand using a pipetting head
  • FIG. 3 shows a possible drive mechanism for the pipetting process.
  • FIG. 1 shows the functional structure of an embodiment of the inventive device.
  • Piston-cylinder units 1 are arranged like a grid, such that the piston 1 ′ is located on a piston plate 2 and the cylinder 1 ′′ on a cylinder plate 3 .
  • the cylinders 1 ′′ of the embodiment shown are formed by providing the cylinder plate 3 with corresponding bores. In this fashion, a suction motion is generated by an upward motion of the piston plate 2 and a discharge motion is generated by a downward motion of the piston plate 2 .
  • each of them may be provided with a sealing ring 31 .
  • a piston-cylinder unit 1 with piston 1 ′, sealing ring 31 and cylinder 1 ′′ is shown in dashed lines as an example.
  • a plurality of such units 1 are provided in a grid-shaped arrangement.
  • a grid of 96 piston-cylinder units 1 may be selected to handle the working positions on these working plates 16 .
  • other grids may also be provided.
  • An adjusting motion 35 of a drive 6 moves the piston plate 2 to a lower stop position 10 .
  • the piston-cylinder units 1 are ready for suctioning a liquid.
  • the drive 6 must now only be deactivated or the locking (not shown) must be released to permit an upward return motion of the piston plate 2 by the restoring springs 34 to an upper stop position 9 .
  • the motion path can be predetermined through corresponding measures such that it can be repeated.
  • a liquid is suctioned in.
  • the piston plate 2 is moved again in a downward direction, the liquid is once more discharged.
  • exact positioning of the piston plate 2 relative to the cylinder plate 3 in the upper stop position 9 and the lower stop position 10 ensures that the volumes which are suctioned and dispensed by all piston-cylinder units 1 are exactly the same.
  • An upper stop 4 and a lower stop 5 ensure this.
  • the stops 4 and 5 each consist of stop points 4 ′, 4 ′′, 4 ′′′ and 4 ′′′′ or stop points 5 ′, 5 ′′, 5 ′′′, and 5 ′′′′ which abut the outer areas of the piston plate 2 , thereby providing good parallel alignment thereof.
  • stop points 4 ′′′ and 4 ′′′′, 5 ′′′, 5 ′′′′ are not shown: the arrows indicate their position).
  • the lower stop points 5 ′, 5 ′′, 5 ′′′, and 5 ′′′′ can only be adjusted to obtain exact parallel alignment of the piston plate 2 in the lower support position 10 .
  • These stop points 5 ′, 5 ′′, 5 ′′′, and 5 ′′′′ may also be designed to form the stop 5 in the illustrated stop position 10 but may be urged downwards under an increased force to provide the above-mentioned additional blow-out function, i.e. to remove residual adhering liquid.
  • the upper stop points 4 ′, 4 ′′, 4 ′′′ and 4 ′′′′ can be set and simultaneously also adjusted to set the volumes to be received and dispensed.
  • This is effected by a transmission 11 which simultaneously adjusts the stops relative to the stop points 4 ′, 4 ′′, 4 ′′′ and 4 ′′′′.
  • This transmission 11 consists of one adjusting means 8 comprising an adjusting wheel 47 , which can drive a drive wheel 44 .
  • the adjusted volume is displayed on the volume display 45 .
  • the drive wheel 44 drives a toothed belt 42 via the adjustment wheel 47 , the toothed belt 42 , in turn, simultaneously driving drive gears 43 .
  • These drive wheels 43 are connected to adjustment nuts 48 which are disposed on the threaded spindles 41 , such that their heights are simultaneously adjusted.
  • These adjustment nuts 48 form stop points 4 ′, 4 ′′, 4 ′′′ and 4 ′′′′ at their lower ends, such that all adjustment points 4 ′, 4 ′′, 4 ′′′ and 4 ′′′′ can be simultaneously adjusted in this fashion, each adjustment ensuring an upper stop position 9 with exactly parallel surfaces to the piston plate 2 relative to the cylinder plate 3 .
  • the drive wheel 44 may alternatively also be a drive gear 43 .
  • receiving means 25 are provided at the lower end of the cylinders 1 ′′ onto which the liquid receptacles can be disposed, which are preferably pipette tips 26 .
  • the illustrated unit is moved exactly over a pipette tip support 28 for receiving such pipette tips 26 .
  • the receiving means 25 accept the pipette tips 26 contained in the pipette tip support 28 .
  • elastic rings 27 are disposed which compensate for even small dimensional differences to always ensure the required retention force.
  • a liquid receptacle or pipette tip wiper 30 is moreover shown which is designed as a perforated plate and is illustrated in a position below the elastic rings 27 , i.e. pipette tips 26 have been ejected.
  • the pipette tip wiper 30 is moved in an upward direction e.g. until it abuts the cylinder plate 3 .
  • the pipette tips 26 can then be disposed on the receptacles 25 and are retained at the elastic rings 27 since they have a slightly smaller diameter than the latter.
  • the pipette tip wiper 30 is moved again into the illustrated position, the pipette tips 26 are swept off and fall e.g. into a support provided for this purpose.
  • the holes of the perforated plate must be larger than the receiving means 25 , including the elastic rings 27 , but smaller than the diameters of the pipette tips 26 to perform this removal function.
  • FIG. 2 shows the arrangement of the device shown in FIG. 1 on a stand 14 .
  • the device of FIG. 1 is in a pipetting head 7 , wherein a drive mechanism 6 for the pipetting process is disposed above the pipetting head 7 , which can be operated using a pivotable hand lever 22 and a fixed retaining handle 32 .
  • the stand 14 consists of a base plate 49 and a structure 50 on which the pipetting head 7 is disposed using a vertical guidance 13 .
  • the pipetting head 7 can be displaced in this vertical guidance 13 such that liquid receptacles such as pipetting tips 26 can be received by a liquid receptacle or pipette tip support 28 such that liquid is also suctioned into the pipette tips 26 , wherein this liquid can, in turn, be dispensed to another working plate 16 , having working positions 33 , e.g. depressions into which the liquid is disposed.
  • lower stops 12 are provided for the pipetting head 7 , which can be adjusted using adjustment screws 21 in correspondence with the respective requirements.
  • the stops 12 are associated with working plates which can be positioned in correspondence with the positioning means 15 , and can be adjusted thereto.
  • a horizontal guidance 17 for the pipetting head 7 is moreover provided to displace it between the two illustrated working plates 16 which are positioned using the positioning means 15 .
  • both ends of the horizontal guidance 17 have positioning aids 18 which are designed as stops.
  • two displaceable trays 51 are disposed on the base plate 49 using horizontal guidances 19 which each contain two positioning means 15 for working plates 16 .
  • These positioning means 15 may e.g. be depressions into which the working plates 16 exactly fit.
  • the trays 51 can be respectively displaced on the horizontal guidances 19 between two stops 20 which are located at the ends of these horizontal guidances 19 such that each end position produces precise grid conformity between a working plate 16 in the positioning means 15 and the pipetting head 7 .
  • Adjustable positioning means 15 for positioning working plates 16 of different sizes are of course also possible.
  • the plates 2 , 3 or the pipetting head 7 may be exchangeable and the positioning aids 18 , 20 may be adjustable for different grids.
  • a transmission lever 29 is provided.
  • This transmission lever 29 is pivotably disposed on the pipetting head 7 via a hinge 36 and has a support 37 into which the end of the transmission lever 29 engages to force the pipetting head 7 with increased force in a downward direction such that the receiving means 25 engage and capture the entire grid of pipetting tips 26 .
  • the transmission lever 29 one side of which is shown, may clearly also be disposed on both sides of the pipetting head 7 , wherein a transverse bar at the ends provides common actuation.
  • FIG. 3 shows one variant of a drive mechanism 6 for the pipetting process.
  • the hand lever 22 is thereby hinged to the pipetting head 7 via a hinge 23 , and transmits the pivot motion to a toothed wheel 39 , which may also be designed as toothed wheel segment, via a transmission rod 38 . It engages in a toothed rack 40 which is connected to the drive rod 46 (shown in FIG. 1 ).
  • the double arrow 35 indicates the adjusting motion to the piston plate 2 . This obtains corresponding transmission 24 between the hand lever 22 and the adjusting motion 35 of the piston plate 2 .
  • FIG. 1 The illustration clearly shows only one feasible embodiment of the invention. It could have a simpler construction e.g. in the form of a device as shown in FIG. 1 which can be moved above a working plate 16 without stand 14 using a corresponding structure. It would also be possible to adjust the stop 4 or the stop 5 by stepping motors or provide a simple motor drive instead of the hand lever actuation.
  • the cylinder plate 3 could, of course, also be moved instead of the piston plates 2 . In this case, a working plate 16 to be operated would have to be moved as well to maintain the correct working distance.
  • intermediate stops could be provided between the stops 4 and 5 , which lock like latches and ensure that the received liquid can be dispensed sequentially in several portions. Further modifications are feasible.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Nozzles (AREA)
  • External Artificial Organs (AREA)
US11/578,826 2004-05-10 2005-04-30 Device for Receiving and Dispensing Liquids Abandoned US20070221684A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE102004023517 2004-05-10
DE102004023517.1 2004-05-10
DE102004023690.9 2004-05-13
DE102004023690 2004-05-13
PCT/EP2005/004694 WO2005113149A1 (de) 2004-05-10 2005-04-30 Vorrichtung zur aufnahme und abgabe von flüssigkeiten

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US20070221684A1 true US20070221684A1 (en) 2007-09-27

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US12/801,552 Active 2027-12-16 US8685342B2 (en) 2004-05-10 2010-06-15 Device for receiving and dispensing liquids

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EP (1) EP1753536B1 (ja)
JP (1) JP4638909B2 (ja)
AT (1) ATE388759T1 (ja)
DE (2) DE502005003212D1 (ja)
PL (1) PL1753536T3 (ja)
WO (1) WO2005113149A1 (ja)

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8367022B2 (en) 2010-05-03 2013-02-05 Integra Biosciences Corp. Unintended motion control for manually directed multi-channel electronic pipettor
US8372356B2 (en) 2010-05-03 2013-02-12 Integra Biosciences Corp. Manually directed, multi-channel electronic pipetting system
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US20140004020A1 (en) * 2012-06-29 2014-01-02 Molecular Bioproducts, Inc. Stand for use with affinity capture
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CN109416371A (zh) * 2016-06-29 2019-03-01 埃佩多夫股份公司 计量头、包括计量头的计量装置和通过计量头进行计量的方法
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CN114733590A (zh) * 2022-05-16 2022-07-12 苏州贝康医疗器械有限公司 移液装置及移液工作站

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DE202005006970U1 (de) 2005-07-14
EP1753536A1 (de) 2007-02-21
WO2005113149A1 (de) 2005-12-01
JP2007537427A (ja) 2007-12-20
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US8685342B2 (en) 2014-04-01
ATE388759T1 (de) 2008-03-15

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