US20060030036A1 - Chips for multiplex analyses - Google Patents

Chips for multiplex analyses Download PDF

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US20060030036A1
US20060030036A1 US11/136,841 US13684105A US2006030036A1 US 20060030036 A1 US20060030036 A1 US 20060030036A1 US 13684105 A US13684105 A US 13684105A US 2006030036 A1 US2006030036 A1 US 2006030036A1
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Prior art keywords
chip
well
reaction
micro
micro well
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US11/136,841
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Victor Joseph
Amjad Huda
Alnoor Shivji
Jie Zhou
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Wafergen Inc
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Wafergen Inc
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Priority to US11/136,841 priority Critical patent/US20060030036A1/en
Assigned to WAFERGEN, INC. reassignment WAFERGEN, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: HUDA, AMJAD, JOSEPH, VICTOR, SHIVJI, ALNOOR, ZHOU, JIE
Publication of US20060030036A1 publication Critical patent/US20060030036A1/en
Abandoned legal-status Critical Current

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    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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Definitions

  • the apparatus of the present invention is capable of performing a vast diversity of chemical reactions.
  • the subject apparatus is particularly suited for performing enzymatic reactions, including but not limited to nucleic acid amplification reaction that encompasses PCR, quantitative polymerase chain reaction (qPCR), nucleic acid sequencing, ligase chain polymerase chain reaction (LCR-PCR), reverse transcription PCR reaction (RT-PCR), reverse transcription, and nucleic acid ligation.
  • qPCR quantitative polymerase chain reaction
  • LCR-PCR ligase chain polymerase chain reaction
  • RT-PCR reverse transcription PCR reaction
  • nucleic acid ligation including but not limited to nucleic acid amplification reaction that encompasses PCR, quantitative polymerase chain reaction (qPCR), nucleic acid sequencing, ligase chain polymerase chain reaction (LCR-PCR), reverse transcription PCR reaction (RT-PCR), reverse transcription, and nucleic acid ligation.
  • FIG. 21 depicts the SYBR Green staining of G6PDH gene products appeared at the three different thermal stages of one PCR cycle. The three stages are primer annealing at 45° C., denaturation of DNA at 95° C., and primer-dependent extension at 72° C.
  • a “primer” is a short polynucleotide, generally with a free 3′-OH group, that binds to a target nucleic acid (or template) potentially present in a sample of interest by hybridizing with the target nucleic acid, and thereafter promoting polymerization of a polynucleotide complementary to the target.
  • any thermo-controllable units in the upper and/or the bottom arrays may be sealed or unsealed.
  • any thermo-controllable units within the upper and/or the bottom arrays may be filled or unfilled, with or without the reaction sample. Leaving one or more thermo-controllable units unfilled enhances heat insulation because air is a poor conductor of heat. Accordingly, it is preferable to leave the adjacent thermo-controllable units empty so as to reduce heat transfer from one thermo-controllable unit to the next unit. It is also preferable to leave the entire upper or the bottom array of thermo-controllable units empty in order to minimize heat transfer from one layer to the next.
  • Micro-heaters are typically made of materials having high thermal conductivity and chemical stability. Such materials include but are not limited to metals such as chromium, platinum and gold, and semi-conductors such as ceramic, silicon, and geranium.
  • a material particularly suitable for fabricating the micro-heaters is indium tin oxide (ITO). ITO is a transparent ceramic material with a very high electrical conductivity. Because ITO can be prepared in bulk or in form of thin layer, it is particularly useful as either an integral or an external heating element.
  • the temperature sensor may be included as a detachable unit located adjacent to or at the base of the thermo-controllable unit. It can also be integrated into the interior or the exterior surface of the unit. Furthermore, the temperature sensor can be fabricated as an integral part of the micro-heater.
  • This configuration is particularly suited for a range of spectroscopic applications because it permits the application of a wide range of excitation wavelengths to a reaction sample being examined.
  • the configuration supports analyses of fluorescence, chemiluminescence, scintillation, bioluminescence, and time-resolved applications without the need for frequent re-alignment of the excitation sources that provide the appropriate excitation wavelengths.
  • Non-limiting examples of this category of biological entities include RNA viruses such as HIV and hepatitis-causing viruses.
  • Another important application of RT-PCR embodied by the present invention is the simultaneous quantification of biological entities based on the mRNA level detected in the test sample.
  • One of skill in the art will appreciate that if a quantitative result is desired, caution must be taken to use a method that maintains or controls for the relative copies of the amplified nucleic acids.

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  • Crystallography & Structural Chemistry (AREA)
  • Robotics (AREA)
  • Manufacturing & Machinery (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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US11/136,841 2004-05-28 2005-05-24 Chips for multiplex analyses Abandoned US20060030036A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US11/136,841 US20060030036A1 (en) 2004-05-28 2005-05-24 Chips for multiplex analyses

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US85755204A 2004-05-28 2004-05-28
US63078904P 2004-11-24 2004-11-24
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US20060030037A1 (en) 2006-02-09
WO2005118773A3 (en) 2006-02-02
EP1758981A2 (de) 2007-03-07
US20180135106A1 (en) 2018-05-17
WO2005118773A2 (en) 2005-12-15
US20160237477A1 (en) 2016-08-18
US20060073491A1 (en) 2006-04-06
US20200299751A1 (en) 2020-09-24
US9909171B2 (en) 2018-03-06
US7622296B2 (en) 2009-11-24
US20100233698A1 (en) 2010-09-16
US20060027317A1 (en) 2006-02-09
US10718014B2 (en) 2020-07-21
US7311794B2 (en) 2007-12-25
US20180135107A1 (en) 2018-05-17
US20060030035A1 (en) 2006-02-09
US20160138082A1 (en) 2016-05-19
US7833709B2 (en) 2010-11-16
US9228933B2 (en) 2016-01-05

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