US20020037512A1 - Methods and reagents for preservation of DNA in bodily fluids - Google Patents

Methods and reagents for preservation of DNA in bodily fluids Download PDF

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Publication number
US20020037512A1
US20020037512A1 US09/805,785 US80578501A US2002037512A1 US 20020037512 A1 US20020037512 A1 US 20020037512A1 US 80578501 A US80578501 A US 80578501A US 2002037512 A1 US2002037512 A1 US 2002037512A1
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Prior art keywords
nucleic acid
dna
amount
group
sodium
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Abandoned
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US09/805,785
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English (en)
Inventor
Tony Baker
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Sierra Diagnostics Inc
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Sierra Diagnostics Inc
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Publication date
Application filed by Sierra Diagnostics Inc filed Critical Sierra Diagnostics Inc
Priority to US09/805,785 priority Critical patent/US20020037512A1/en
Priority to US09/882,871 priority patent/US6458546B1/en
Priority to US09/932,122 priority patent/US7569342B2/en
Publication of US20020037512A1 publication Critical patent/US20020037512A1/en
Priority to US11/774,985 priority patent/US20080064108A1/en
Priority to US12/484,788 priority patent/US20090305422A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Definitions

  • FIG. 1 is a graph of DNA concentration in unpreserved urine according to the prior art
  • FIG. 12 graphically illustrates a comparison of signal response in PCR assays wherein the DNA has been treated with a preservative of the invention, and one which has not;
  • the invention relates to nucleic acid preservative solutions comprising an amount of a divalent metal chelator selected from EDTA, EGTA and BAPTA, and salts thereof; and an amount of at least one chelator enhancing component selected from lithium chloride, guanidine, sodium salicylate, sodium perchlorate, and sodium thiocyanate.
  • the amount of the divalent metal chelator is generally in the range of from about 0.001M to 0.1M, and the amount of the chelator enhancing component is generally in the range of from about 0.1M to 2M.
  • the amount of chelator enhancing component is more desirably at least 1M in the preservative solution, and the divalent metal chelator is desirably present in an amount of at least about 0.01M.
  • FIG. 7 is a graph comparing PCR results in unpreserved and preserved normal urine according to the invention.
  • a MOMP template to Chlamydia trachomatis was used and amplified using a standard PCR protocol. 200 copies of the MOMP target were spiked into 9 ml of fresh human urine containing 1 M sodium perchlorate and 0.01M BAPTA. PCR was done each hour for eight hours total. In the unprotected urine, approximately three PCR absorbances were measured one hour after the addition of DNA to the urine. The number of PCR absorbances approached zero by the sixth hour. By contrast, in the preserved specimen, in excess of three PCR absorbances were measured at the one hour testing.
  • a suspension of gonococci was immediately added to each urine specimen.
  • the added gonococci were an ordinary strain of N. Gonorrhoeae, 49191, which was grown overnight on GC agar medium at 37° C. in a 5% CO 2 atmosphere.
  • the N. Gonorrhoeae colonies were picked and suspended in GC buffer.
  • a ⁇ fraction (1/10) ⁇ volume of a suspension containing approximately 10 Colony forming units (cfu) per ml was added to the urine.
  • the suspension of gonococci was also added to Hepes buffer.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
US09/805,785 1997-12-10 2001-03-13 Methods and reagents for preservation of DNA in bodily fluids Abandoned US20020037512A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US09/805,785 US20020037512A1 (en) 1997-12-10 2001-03-13 Methods and reagents for preservation of DNA in bodily fluids
US09/882,871 US6458546B1 (en) 1997-12-10 2001-06-15 Methods and reagents for preservation of DNA in bodily fluids
US09/932,122 US7569342B2 (en) 1997-12-10 2001-08-16 Removal of molecular assay interferences
US11/774,985 US20080064108A1 (en) 1997-12-10 2007-07-09 Urine Preservation System
US12/484,788 US20090305422A1 (en) 1997-12-10 2009-06-15 Methods and reagents for preservation of dna in bodily fluids

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US98802997A 1997-12-10 1997-12-10
US18540298A 1998-11-03 1998-11-03
US09/805,785 US20020037512A1 (en) 1997-12-10 2001-03-13 Methods and reagents for preservation of DNA in bodily fluids

Related Parent Applications (1)

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US18540298A Continuation 1997-12-10 1998-11-03

Related Child Applications (2)

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US09/882,871 Continuation US6458546B1 (en) 1997-12-10 2001-06-15 Methods and reagents for preservation of DNA in bodily fluids
US09/932,122 Continuation-In-Part US7569342B2 (en) 1997-12-10 2001-08-16 Removal of molecular assay interferences

Publications (1)

Publication Number Publication Date
US20020037512A1 true US20020037512A1 (en) 2002-03-28

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US09/805,785 Abandoned US20020037512A1 (en) 1997-12-10 2001-03-13 Methods and reagents for preservation of DNA in bodily fluids
US09/882,871 Expired - Lifetime US6458546B1 (en) 1997-12-10 2001-06-15 Methods and reagents for preservation of DNA in bodily fluids

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US09/882,871 Expired - Lifetime US6458546B1 (en) 1997-12-10 2001-06-15 Methods and reagents for preservation of DNA in bodily fluids

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US (2) US20020037512A1 (de)
EP (1) EP1038028A2 (de)
JP (1) JP2001526051A (de)
AU (1) AU1719699A (de)
CA (1) CA2313654A1 (de)
WO (1) WO1999029904A2 (de)

Cited By (7)

* Cited by examiner, † Cited by third party
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KR101039521B1 (ko) * 2008-02-01 2011-06-08 주식회사 인트론바이오테크놀로지 금속 이온에 의해 발생하는 ｐｃｒ 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 ｐｃｒ 반응용 조성물
US20120288956A1 (en) * 2008-09-05 2012-11-15 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
US20170130219A1 (en) * 2014-04-10 2017-05-11 Dna Genotek Inc. Method and system for microbial lysis using periodates
CN110029102A (zh) * 2019-04-21 2019-07-19 湖南大地同年生物科技有限公司 一种粪便脱落细胞及其核酸保存试剂及其制备和应用
CN112522360A (zh) * 2020-02-06 2021-03-19 博尔诚（北京）科技有限公司 一种组合物、采样装置、试剂盒及居家病毒检测方法
US11002646B2 (en) 2011-06-19 2021-05-11 DNA Genotek, Inc. Devices, solutions and methods for sample collection
US11572581B2 (en) 2002-06-07 2023-02-07 DNA Genotek, Inc. Compositions and methods for obtaining nucleic acids from sputum

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US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
US20060014214A1 (en) * 2004-05-25 2006-01-19 Sierra Diagnostics, Llc Urine preservation system
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
DE10031236A1 (de) 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
EP1207208A3 (de) * 2000-11-15 2003-12-10 Becton Dickinson and Company Methode zur Erhaltung von Zellen und Nukleinsäurezielmolekülen
US7029840B2 (en) 2000-11-15 2006-04-18 Becton, Dickinson And Company Method for preservation of cells and nucleic acid targets
WO2008111981A1 (en) * 2007-03-14 2008-09-18 Sierra Molecular Corporation Compositions, systems, and methods for preservation of macromolecules
US20100120078A1 (en) * 2001-08-16 2010-05-13 Tony Baker Urine Stabilization System
DE10261529A1 (de) * 2002-12-23 2004-07-08 Indivumed Gmbh Verfahren zur Erstellung einer Sammlung von biologischem Probenmaterial und Probensammlung
EP1584923A3 (de) * 2004-04-07 2006-01-04 Roche Diagnostics GmbH Stabilisierung von Biomolekülen in Proben
US20080146790A1 (en) * 2004-08-18 2008-06-19 Daniel Grolz Additive, Method, and Article For Dna Collection, Stabilization, and Purification
US20070134266A1 (en) * 2005-08-26 2007-06-14 Johnson Louis B Growth hormone-containing formulation and method of use
US8138228B2 (en) * 2004-09-16 2012-03-20 Accelegrow Technologies, Inc. Liquid kelp formulation with or without enhanced shelf life, and method of making
US20070275009A1 (en) * 2005-08-26 2007-11-29 Johnson Louis R Dry fertilizer with growth hormone-containing formulation and methods of use and making
WO2008097501A2 (en) * 2007-02-02 2008-08-14 Accelegrow Technologies, Inc. Method of improving plant growth and plant growth composition
US20060053851A1 (en) * 2004-09-16 2006-03-16 Johnson Louis B Liquid kelp formulation with or without enhanced shelf life, and method of making
US8133919B2 (en) * 2004-09-16 2012-03-13 Accelegrow Technologies, Inc. Dry fertilizer with growth hormone-containing formulation and methods of use and making
CA2600758C (en) 2005-03-16 2016-01-12 Dna Genotek Inc. Compositions and method for storage of nucleic acid from bodily fluids
CA2632614C (en) 2005-12-09 2014-04-01 Dna Genotek Inc. Container system for releasably storing a substance
EP2076595A2 (de) * 2006-09-12 2009-07-08 Sierra Molecular Corporation Entfernung molekularer assay-interferenzen für nukleinsäuren unter einsatz gepufferter chaotroplösungen
EP2129780A2 (de) * 2007-03-14 2009-12-09 Sierra Molecular Corporation Zusammensetzungen, systeme und verfahren zur konservierung und/oder stabilisierung einer zelle und/oder eines makromoleküls
EP2247753B1 (de) * 2008-02-01 2014-06-25 Siemens Healthcare Diagnostics Inc. Urintransportmittel
WO2010003037A2 (en) * 2008-07-03 2010-01-07 Sierra Molecular Corporation Compositions, systems, and methods for stabilization of a cell and/or macromolecule
US9415032B2 (en) 2011-10-05 2016-08-16 The Johns Hopkins University Membrane activated chelators and use in the prevention and treatment of parasitic infection
WO2013147043A1 (ja) * 2012-03-29 2013-10-03 株式会社シノテスト 核酸の検出又は定量方法及び検出又は定量用試薬キット
SI2970878T1 (sl) 2013-03-15 2019-02-28 Truckee Applied Genomics, Llc Postopki in reagenti za vzdrževanje preživetja rakavih celic v kirurško odstranjenem tkivu
WO2017169770A1 (ja) * 2016-03-28 2017-10-05 富士フイルム株式会社 細胞分析システム
JP6733951B2 (ja) * 2016-05-09 2020-08-05 日本メナード化粧品株式会社 核酸保存用組成物及び核酸保存方法
DK3568475T3 (da) 2017-01-16 2023-04-17 Spectrum Solutions L L C Nukleinsyrekonserveringsopløsning og fremgangsmåder til anvendelse
MX2019010800A (es) 2017-03-15 2020-07-28 Ancestry Com Dna Llc Dispositivo y metodo de recoleccion de muestras.
CA3078493A1 (en) 2017-10-06 2019-04-11 Ancestry.Com Dna, Llc Systems, devices, and methods for sample collection
EP3713498A4 (de) 2017-11-22 2021-08-04 Ancestry.com DNA, LLC Probensammlungskit mit kappe mit selektiv beweglicher hülse
US11426734B2 (en) 2017-11-22 2022-08-30 Ancestry.Com Dna, Llc Sample collection kit including cap having selectively movable sleeve
WO2020102570A1 (en) * 2018-11-14 2020-05-22 Spectrum Solutions L.L.C. Rna preservation solution and methods of manufacture and use
EP3884070A4 (de) 2018-11-20 2022-09-07 Spectrum Solutions, LLC Probensammelsystem mit verschlusskappe und ventil
CA3131344A1 (en) 2019-02-27 2020-09-03 Ancestry.Com Dna, Llc Graphical user interface displaying relatedness based on shared dna
US11701094B2 (en) 2019-06-20 2023-07-18 Spectrum Solutions L.L.C. Sample collection system including valve and plug assemblies
WO2021055170A1 (en) * 2019-09-17 2021-03-25 Longhorn Vaccines And Diagnostics, Llc Multipurpose compositions for collecting and transporting biological material
US12332902B2 (en) 2022-04-20 2025-06-17 Ancestry.Com Dna, Llc Filtering individual datasets in a database

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WO1995035390A1 (en) * 1994-06-22 1995-12-28 Mount Sinai School Of Medicine Of The City University Of New York Ligation-dependent amplification for the detection of infectious pathogens and abnormal genes

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11572581B2 (en) 2002-06-07 2023-02-07 DNA Genotek, Inc. Compositions and methods for obtaining nucleic acids from sputum
KR101039521B1 (ko) * 2008-02-01 2011-06-08 주식회사 인트론바이오테크놀로지 금속 이온에 의해 발생하는 ｐｃｒ 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 ｐｃｒ 반응용 조성물
US20120288956A1 (en) * 2008-09-05 2012-11-15 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
US8722330B2 (en) * 2008-09-05 2014-05-13 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
US20140205517A1 (en) * 2008-09-05 2014-07-24 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
US11536632B2 (en) 2011-06-19 2022-12-27 DNA Genotek, Inc. Biological collection system
US11002646B2 (en) 2011-06-19 2021-05-11 DNA Genotek, Inc. Devices, solutions and methods for sample collection
US11549870B2 (en) 2011-06-19 2023-01-10 DNA Genotek, Inc. Cell preserving solution
US11592368B2 (en) 2011-06-19 2023-02-28 DNA Genotek, Inc. Method for collecting and preserving a biological sample
US11046949B2 (en) * 2014-04-10 2021-06-29 Dna Genotek Inc. Method and system for microbial lysis using periodates
US20170130219A1 (en) * 2014-04-10 2017-05-11 Dna Genotek Inc. Method and system for microbial lysis using periodates
CN110029102A (zh) * 2019-04-21 2019-07-19 湖南大地同年生物科技有限公司 一种粪便脱落细胞及其核酸保存试剂及其制备和应用
CN112522360A (zh) * 2020-02-06 2021-03-19 博尔诚（北京）科技有限公司 一种组合物、采样装置、试剂盒及居家病毒检测方法

Also Published As

Publication number Publication date
US6458546B1 (en) 2002-10-01
WO1999029904A2 (en) 1999-06-17
EP1038028A2 (de) 2000-09-27
WO1999029904A3 (en) 1999-12-23
CA2313654A1 (en) 1999-06-17
JP2001526051A (ja) 2001-12-18
AU1719699A (en) 1999-06-28
US20020102570A1 (en) 2002-08-01

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