US10143989B2 - Stabilisation features - Google Patents

Stabilisation features Download PDF

Info

Publication number
US10143989B2
US10143989B2 US14/761,470 US201414761470A US10143989B2 US 10143989 B2 US10143989 B2 US 10143989B2 US 201414761470 A US201414761470 A US 201414761470A US 10143989 B2 US10143989 B2 US 10143989B2
Authority
US
United States
Prior art keywords
vessel
cross
vertical axis
stabilization feature
sectional shape
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active, expires
Application number
US14/761,470
Other languages
English (en)
Other versions
US20160045883A1 (en
Inventor
Brian Barrett
Patrick Tuohy
Caitriona Ryan
Mark McCabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GenCell Biosystems Ltd
Original Assignee
GenCell Biosystems Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GenCell Biosystems Ltd filed Critical GenCell Biosystems Ltd
Priority to US14/761,470 priority Critical patent/US10143989B2/en
Assigned to GENCELL BIOSYSTEMS LTD. reassignment GENCELL BIOSYSTEMS LTD. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BARRETT, BRIAN, MCCABE, MARK, RYAN, Caitriona, TUOHY, PATRICK
Publication of US20160045883A1 publication Critical patent/US20160045883A1/en
Application granted granted Critical
Publication of US10143989B2 publication Critical patent/US10143989B2/en
Active legal-status Critical Current
Adjusted expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/00756—Compositions, e.g. coatings, crystals, formulations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates

Definitions

  • FIGS. 1-12 schematically show various liquid cell stabilisation features from above, and in side cross-section, in some cases including stabilized CLCs.
  • FIGS. 7 and 8 schematically show a generally diamond-shaped stabilisation feature, where the short axis of the diamond retains the CLC, and the long axis of the diamond defines channels through which gases may escape.
  • FIGS. 9 and 10 schematically show a stabilisation feature similar to that of FIGS. 1 and 2 .
  • control surfaces have a circular shape.
  • the stabilisation feature consists of circular through-hole that is hydrophobic.
  • the through-hole is generated in a hydrophobic material.
  • stabilisation features can have a variety of shapes and functions. Some are connected by “canals” in a network. In some cases, the canals in a network can serve the same function to encourage the exit of gases as the channels shown in FIGS. 1-12 .
  • control surfaces are shaped to allow gases to escape, while maintaining the composite liquid cell in the optimum position for processing, for example, in liquid handling, thermal processing, and/or during optical detection.
  • One embodiment of this invention uses the density differences of fluids to ensure gas free operation.
  • the shape is that of a semi-circle, see FIG. 9
  • control surfaces move.
  • control surfaces are heated.
  • the encapsulating fluid is maintained at a thermal set point for biological processing.
  • an encapsulating fluid is deposited on the free surface of a carrier fluid.
  • the encapsulating fluid is then agitated.
  • a biological sample is then dispensed, generating a composite liquid cell.
  • the agitation is pressure induced.
  • a stabilization feature can be used to immobilize a composite liquid cell.
  • the stabilization feature can include a hydrophobic wall defining a vessel.
  • the vessel can define a central portion sized and shaped to snugly contain a composite liquid of a predetermined size, a vertical axis, and at least one channel portion adapted to flow gas away from the central portion.
  • the vessel generally defines a cross-sectional shape in a plane perpendicular to the vertical axis. In some embodiments the cross-sectional shape is constant along the vertical axis. In some embodiments the cross-sectional shape is not constant along the vertical axis.
  • the vessel may be tapered so that the cross-sectional shape is larger at or near one end of the vessel than at or near the other end of the vessel.
  • the at least one channel portion can be substantially circular, or substantially rectangular. In some embodiments the at least one channel portion can be offset horizontally from the central portion. In some embodiments the at least one channel portion is a plurality of channel portions, while in other embodiments the at least one channel portion is a single channel portion. A plurality of channel portions can be evenly or unevenly circumferentially spaced around the vertical axis. For example, two channel portions can be spaced 180 degrees apart from one another around the vertical axis, or four channel portions can be spaced 90 degrees apart from one another around the vertical axis.
  • a stabilization feature for immobilizing a composite liquid cell can include a hydrophobic wall defining a vessel.
  • the vessel can define a central portion sized and shaped to snugly contain a composite liquid of a predetermined size, and a vertical axis.
  • the vessel can have a cross-sectional shape in a plane perpendicular to the vertical axis, and the cross-sectional shape can be tapered along the vertical axis so that the area of the cross-sectional shape is larger at a top of the vertical axis than at a bottom of the vertical axis.
  • a plurality of stabilization features can be part of a network.
  • the stabilization features in the network can be connected by a plurality of canals.
  • Each stabilization feature can be in fluid communication with one or more other stabilization features by way of one or more canals.
  • such a network can include a carrier liquid having a substantially horizontal free surface, and the central portion of each vessel can include a predetermined quantity of an encapsulating liquid.
  • the carrier liquid can be immiscible with the encapsulating liquid and denser than the encapsulating liquid so that the encapsulating liquid floats on the free surface of the carrier liquid.
  • a method can include providing such a network that includes stabilization features connected by canals.
  • the method can include charging the plurality of canals and the stabilization features with a sufficient quantity of a carrier liquid that the carrier liquid has a substantially horizontal free surface throughout all the canals and stabilization features, drawing a predetermined quantity of an encapsulating liquid from an encapsulating liquid input, discharging the drawn predetermined quantity of encapsulating liquid proximate to one of the plurality of stabilization features, wherein the carrier liquid is immiscible with the encapsulating liquid and denser than the encapsulating liquid so that the predetermined quantity of encapsulating liquid floats on the free surface of the carrier liquid, drawing a predetermined quantity of a sample liquid from a sample-liquid input, and discharging the drawn predetermined quantity of sample liquid proximate to the discharged predetermined quantity of encapsulating liquid to form a composite liquid cell on the free surface of the carrier liquid, the sample liquid being immiscible with the encapsulating liquid and with
  • such methods can include either heating or cooling the discharged predetermined quantity of encapsulating liquid.
  • the heating or cooling can be carried out either prior to, during, or after discharging the predetermined quantity of sample liquid.
  • the composite liquid cell can be maintained a predetermined thermal set point a predetermined amount of time as part of a biological processing protocol.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
US14/761,470 2013-05-07 2014-05-07 Stabilisation features Active 2035-06-01 US10143989B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/761,470 US10143989B2 (en) 2013-05-07 2014-05-07 Stabilisation features

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361820463P 2013-05-07 2013-05-07
US14/761,470 US10143989B2 (en) 2013-05-07 2014-05-07 Stabilisation features
PCT/IB2014/001784 WO2014188281A2 (en) 2013-05-07 2014-05-07 Stabilisation features

Publications (2)

Publication Number Publication Date
US20160045883A1 US20160045883A1 (en) 2016-02-18
US10143989B2 true US10143989B2 (en) 2018-12-04

Family

ID=51743482

Family Applications (1)

Application Number Title Priority Date Filing Date
US14/761,470 Active 2035-06-01 US10143989B2 (en) 2013-05-07 2014-05-07 Stabilisation features

Country Status (4)

Country Link
US (1) US10143989B2 (zh)
EP (1) EP2994233A2 (zh)
CN (1) CN105283248B (zh)
WO (1) WO2014188281A2 (zh)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9777269B2 (en) 2012-01-25 2017-10-03 Gencell Biosystems Ltd. Biomolecule isolation
EP3011023A2 (en) 2013-06-18 2016-04-27 Gencell Biosystems Ltd. Biomolecule isolation and thermal processing
CN105723203B (zh) 2013-11-25 2019-10-25 基因细胞生物系统有限公司 磁性分离
US9952127B2 (en) 2014-08-04 2018-04-24 Becton, Dickinson And Company Triphasic fluid handling
ES2764276T3 (es) 2014-08-04 2020-06-02 Gencell Biosystems Ltd Dispositivo de análisis genómico y métodos de uso
EP3177393A1 (en) 2014-08-04 2017-06-14 Gencell Biosystems Limited Benchtop nucleic acid library preparation device and methods for using the same
JP6665548B2 (ja) 2015-03-06 2020-03-13 ソニー株式会社 マイクロチップ、並びに分析装置及び分析方法
WO2016143278A1 (en) * 2015-03-06 2016-09-15 Sony Corporation Microchip, analysis apparatus, and analysis method
US20170058277A1 (en) 2015-08-26 2017-03-02 Gencell Biosystems Ltd. Composite liquid cell (clc) supports, and methods of making and using the same

Citations (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5065768A (en) * 1988-09-13 1991-11-19 Safe-Tec Clinical Products, Inc. Self-sealing fluid conduit and collection device
US6184029B1 (en) * 1992-05-01 2001-02-06 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US20020050659A1 (en) * 2000-02-09 2002-05-02 William Toreki Hydrocapsules and method of preparation thereof
JP2005169386A (ja) 2003-11-17 2005-06-30 Kanagawa Acad Of Sci & Technol マイクロチャンネル内表面の部分化学修飾方法とマイクロチャンネル構造体
US20050148066A1 (en) * 2000-02-18 2005-07-07 O'keefe Matthew Apparatus and methods for parallel processing of micro-volume liquid reactions
US20050266582A1 (en) * 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
WO2007024914A2 (en) 2005-08-22 2007-03-01 Applera Corporation Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible
US20070264705A1 (en) * 2003-09-10 2007-11-15 John Dodgson Apparatus and Method for Handling Cells, Embryos or Oocytes
US20100028207A1 (en) * 2008-07-16 2010-02-04 International Technidyne Corporation Cuvette-based apparatus for blood coagulation measurement and testing
US20110003286A1 (en) * 2007-02-05 2011-01-06 Nobuhiro Hanafusa Reactor plate and reaction processing method
US20110226339A1 (en) 2007-12-21 2011-09-22 Konica Minolta Medical & Graphic, Inc., Microchip and liquid sending method for microchip
US8092432B2 (en) 2005-05-03 2012-01-10 Nordgren Corporation Outdwelling slit valves and assemblies for medical liquid flow through a cannula and related methods
WO2012011091A2 (en) 2010-07-22 2012-01-26 Gencell Biosystems Limited Composite liquid cells
US8192841B2 (en) * 2006-12-14 2012-06-05 Kimberly-Clark Worldwide, Inc. Microencapsulated delivery vehicle having an aqueous core
US20130059322A1 (en) * 2005-07-07 2013-03-07 CellASIC, INC. Cell culture and invasion assay method and system
WO2013111016A2 (en) 2012-01-25 2013-08-01 Gencell Biosystems Limited Biomolecule isolation
WO2014083435A2 (en) 2012-11-27 2014-06-05 Gencell Biosystems Ltd. Handling liquid samples
WO2014207577A2 (en) 2013-06-18 2014-12-31 Ell Biosystems Ltd. Biomolecule isolation and thermal processing
WO2015075560A2 (en) 2013-11-25 2015-05-28 Gencell Biosystems Ltd. Transportable composite liquid cells
WO2015075563A2 (en) 2013-11-25 2015-05-28 Gencell Biosystems Ltd. Magnetic separation

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1987480A (zh) * 2005-12-23 2007-06-27 博奥生物有限公司 用移液器的吸头给亲水性微细管道加入流体样品的加样口

Patent Citations (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5065768A (en) * 1988-09-13 1991-11-19 Safe-Tec Clinical Products, Inc. Self-sealing fluid conduit and collection device
US6184029B1 (en) * 1992-05-01 2001-02-06 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US20020050659A1 (en) * 2000-02-09 2002-05-02 William Toreki Hydrocapsules and method of preparation thereof
US20050148066A1 (en) * 2000-02-18 2005-07-07 O'keefe Matthew Apparatus and methods for parallel processing of micro-volume liquid reactions
US20050266582A1 (en) * 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
US20070264705A1 (en) * 2003-09-10 2007-11-15 John Dodgson Apparatus and Method for Handling Cells, Embryos or Oocytes
JP2005169386A (ja) 2003-11-17 2005-06-30 Kanagawa Acad Of Sci & Technol マイクロチャンネル内表面の部分化学修飾方法とマイクロチャンネル構造体
US8092432B2 (en) 2005-05-03 2012-01-10 Nordgren Corporation Outdwelling slit valves and assemblies for medical liquid flow through a cannula and related methods
US20130059322A1 (en) * 2005-07-07 2013-03-07 CellASIC, INC. Cell culture and invasion assay method and system
WO2007024914A2 (en) 2005-08-22 2007-03-01 Applera Corporation Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible
WO2007024798A2 (en) 2005-08-22 2007-03-01 Applera Corporation Apparatus, system, and method using immiscible-fluid-discrete-volumes
WO2007024800A2 (en) 2005-08-22 2007-03-01 Applera Corporation Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
US9194772B2 (en) 2005-08-22 2015-11-24 Applied Biosystems, Llc Apparatus, system, and method using immiscible-fluid-discrete-volumes
WO2007024778A2 (en) 2005-08-22 2007-03-01 Applera Corporation Device, system and method for depositing processed immiscible-fluid-discrete-volumes
US8192841B2 (en) * 2006-12-14 2012-06-05 Kimberly-Clark Worldwide, Inc. Microencapsulated delivery vehicle having an aqueous core
US20110003286A1 (en) * 2007-02-05 2011-01-06 Nobuhiro Hanafusa Reactor plate and reaction processing method
US20110226339A1 (en) 2007-12-21 2011-09-22 Konica Minolta Medical & Graphic, Inc., Microchip and liquid sending method for microchip
US20100028207A1 (en) * 2008-07-16 2010-02-04 International Technidyne Corporation Cuvette-based apparatus for blood coagulation measurement and testing
US20120045765A1 (en) * 2010-07-22 2012-02-23 Gencell Biosystems Ltd. Composite liquid cells
WO2012011091A2 (en) 2010-07-22 2012-01-26 Gencell Biosystems Limited Composite liquid cells
WO2013111016A2 (en) 2012-01-25 2013-08-01 Gencell Biosystems Limited Biomolecule isolation
WO2014083435A2 (en) 2012-11-27 2014-06-05 Gencell Biosystems Ltd. Handling liquid samples
WO2014207577A2 (en) 2013-06-18 2014-12-31 Ell Biosystems Ltd. Biomolecule isolation and thermal processing
WO2015075560A2 (en) 2013-11-25 2015-05-28 Gencell Biosystems Ltd. Transportable composite liquid cells
WO2015075563A2 (en) 2013-11-25 2015-05-28 Gencell Biosystems Ltd. Magnetic separation

Also Published As

Publication number Publication date
US20160045883A1 (en) 2016-02-18
WO2014188281A2 (en) 2014-11-27
CN105283248B (zh) 2018-02-06
WO2014188281A3 (en) 2015-04-16
CN105283248A (zh) 2016-01-27
EP2994233A2 (en) 2016-03-16

Similar Documents

Publication Publication Date Title
US10143989B2 (en) Stabilisation features
US11066699B2 (en) Microfluidic process for treating and analysing a solution containing a biological material and corresponding microfluidic circuit
Zhu et al. Single-molecule emulsion PCR in microfluidic droplets
US9776182B2 (en) Handling liquid samples
EP2061598B1 (en) Method for sampling flowable materials
US20180311671A1 (en) Microfluidic arrangements
WO2016078340A1 (zh) 微量液体分配/混合装置、系统及方法
Postek et al. Microfluidic screening of antibiotic susceptibility at a single-cell level shows the inoculum effect of cefotaxime on E. coli
US20150209786A1 (en) Compositions and methods for dispensing reagents
EP3142790B1 (en) Method for handling fluid in a microfluidic device with channel plates
EP3074131B1 (en) Transportable composite liquid cells
CN103954786A (zh) 一种半接触式的油下液滴连续点样和加液方法
GB2572699A (en) Microfluidic arrangements
AU2020219461B2 (en) Microdroplet manipulation method
US8945486B2 (en) Microwell device
Hu et al. High throughput single cell separation and identification using a self-priming isometric and Equant screw valve-based (SIES) microfluidic chip
JP6803561B2 (ja) マイクロ流路での検体と反応試薬との混合方法
WO2017131951A1 (en) Enhanced composite liquid cell (clc) devices, and methods for using the same
JP2008164512A (ja) 生化学反応容器
CN111500458A (zh) 一种椭圆形深孔板
Ge et al. An integrated microfluidic platform for on-demand single droplet dispenser with high accuracy by electrohydrodynamic (EHD) printing technique
Khor et al. Removing the Ceiling on Droplet Microfluidics

Legal Events

Date Code Title Description
AS Assignment

Owner name: GENCELL BIOSYSTEMS LTD., IRELAND

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BARRETT, BRIAN;TUOHY, PATRICK;RYAN, CAITRIONA;AND OTHERS;REEL/FRAME:036519/0044

Effective date: 20130531

STCF Information on status: patent grant

Free format text: PATENTED CASE

MAFP Maintenance fee payment

Free format text: PAYMENT OF MAINTENANCE FEE, 4TH YEAR, LARGE ENTITY (ORIGINAL EVENT CODE: M1551); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY

Year of fee payment: 4