CN1987480A - 用移液器的吸头给亲水性微细管道加入流体样品的加样口 - Google Patents

用移液器的吸头给亲水性微细管道加入流体样品的加样口 Download PDF

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CN1987480A
CN1987480A CNA2005101307060A CN200510130706A CN1987480A CN 1987480 A CN1987480 A CN 1987480A CN A2005101307060 A CNA2005101307060 A CN A2005101307060A CN 200510130706 A CN200510130706 A CN 200510130706A CN 1987480 A CN1987480 A CN 1987480A
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官晓胜
胡玉明
郭旻
周骋
程京
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Tsinghua University
CapitalBio Corp
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CapitalBio Corp
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Priority to CNA2005101307060A priority Critical patent/CN1987480A/zh
Priority to EP06828382A priority patent/EP1963867A4/en
Priority to PCT/CN2006/003471 priority patent/WO2007071174A1/en
Priority to US12/096,020 priority patent/US20090123338A1/en
Publication of CN1987480A publication Critical patent/CN1987480A/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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Abstract

用移液器的吸头给亲水性微细管道加入流体样品的加样口，属于生物芯片中流体器件技术领域，其特征在于，该加样口包括：位于流体在微细管道内流动方向下游的吸头插入孔，位于流体在微细管道内流动方向上游的隔离导气孔以及贯穿该吸头插头孔和隔离导气孔用的位于侧壁上的一个缩口或一条突起。该吸头插入孔的深度和截面尺寸或形状要足以使该吸头同心且稳定地插入到最大深度而又不会与吸头插入孔的底面接触，而且吸头插入孔的截面周长和截面面积之比要小于微细管道截面周长与截面面积之比。该加样口不会使加入的流体样品在微细管道内分段。

Description

用移液器的吸头给亲水性微细管道加入流体样品的加样口
技术领域
本发明涉及一种流体器件的结构设计。
背景技术
许多缩微和集成化了的分析仪器和设备(又称微型全分析系统或芯片实验室)都会遇到和外部的宏观世界接口的问题，其中之一就是如何做到方便、准确，和可靠地将定量的化学或生物样品和试剂加入到这些系统中去。传统的手持式移液器作为一种成熟的并具有较高定量精度的加样工具，已为广大的分析操作人员所熟悉和使用，因此也经常成为给微型全分析系统加样的首选工具；但是，在实际操作中加样效果往往不理想。一般来说，微型全分析系统多为亲水性材料制成的管道或腔体，其中管道的尺寸在几十微米到几百微米之间，加样过程通过一个和移液器的吸头对接的加样口完成。理想的情况是，样品从吸头打出进入加样口后，会在虹吸原理的作用下被自动吸进和加样口连接的微管道内。为了方便吸头的插入和固定，加样口的尺寸略大于吸头出口处的外壁直径。由于它们之间存在有缝隙，而人工操作又很难保证吸头在加样口内保持稳定而不发生抖动，空气因而很容易在此过程中被携带进微管道中。当所加的样品或试剂含有表面活性剂时，这种可能性更是大为增加。另外，当加完样品后，加样口一般应被封闭，以保证流体样品在微细管道内能够被驱动而不发生泄漏。在封闭过程中，加样口内的空气因被略微压缩而极易挤进管道，从而导致加入的样品在微管道内分段。
发明内容
本发明的目的在于提供一种用移液器的吸头给亲水性微细管道加入流体样品的加样口，使用该加样口加样，可以把携带进气泡的可能性降到最低程度。
本发明的特征在于，该加样口由两个盲孔组成，其中：一个盲孔为所述吸头插入孔，位于流体在所述微细管道内流动方向的下游；另一个盲孔为隔离导气孔，位于流体在所述微细管道内流动方向的上游，该隔离导气孔通过侧壁上的一个缩口或一条窄缝与所述吸头插入孔相通，所述微细管道从该隔离导气孔的底部或靠近底部的侧壁处进入，再从吸头插入孔的底部或者靠近底部的侧壁处离开，隔离导气孔位于吸头插入孔的上游方向。所述吸头插入孔的深度和截面的尺寸或形状足以使该吸头插入到最大深度时不会与吸头插入孔的底面相接触，并使吸头得以固定和稳定。所述吸头插入孔截面的周长与面积比小于微细管道截面的周长与面积之比。
实验证明：直接开口的微管道加样时常常混入气泡导致加样失败，而使用本发明所述加样口的微管道的加样不仅操作简单，所加流体中也不会混入气泡。
附图说明
图1.本发明所述加样口的俯视图。
图2.本发明所述加样口与微管道相连的示意图。
具体实施方式
本发明为了解决上述问题，特别设计了一种加样口。首先如图1所示，该加样口由两个盲孔构成，按照流体在微管道内流动的方向设置，其中位于微管道下游(1)一侧的是吸头插入孔(2)，用于插入和固定移液器的吸头，并接受从吸头打出的流体样品或试剂；位于微管道上游(4)一侧的是隔离导气孔(3)，不和所述流体发生接触，隔离导气孔通过一个缩口或窄缝与吸头插入孔连通。这个缩口或窄缝的作用是：一方面能够产生一定的被动阀效应，将由吸头打出的流体限制在吸头插入孔之内，另一方面又允许吸头插入孔内的一部分空气在此过程中能够被排挤到隔离导气孔而释放掉，这样在很大程度上就减少了将气泡携带进微管道的机会。图2是该加样口的立体视图。其次，当封闭加样口时，外力产生的正压会将所述流体进一步完全推入到和吸头插入孔相通的下游微管道内，而不会进入隔离导气孔以及和它相通的上游微管道，因此也就不会产生流体分段现象。为了进一步提高该结构的可靠性，一般还要求微管道的水利直径(管道截面面积的四倍除以截面周长)小于吸头插入孔的水利直径，这样，和吸头插入孔连通的微管道因为表面张力的作用能够产生一定的负压，一方面自动吸入流体样品，另一方面更相对增强了缩口或窄缝的被动阀效应。根据移液器吸头的锥形形状，吸头插入孔的截面尺寸和形状可以进一步作相应的设计：一方面使得吸头能够插入一定深度，并起到最佳的固定和稳定吸头的作用；另一方面又保证吸头始终和吸头插入孔的底部保持一段距离，避免出现毛细现象，将携带进气泡的可能性降低到最低。

Claims (3)

1.用移液器的吸头给亲水性微细管道加入流体样品的加样口，其特征在于，该加样口由两个盲孔组成，其中：
一个盲孔为所述吸头插入孔，位于流体在所述微细管道内流动方向的下游；
另一个盲孔为隔离导气孔，位于流体在所述微细管道内流动方向的上游，该隔离导气孔通过侧壁上的一个缩口或一条窄缝与所述吸头插入孔相通，所述微细管道从该隔离导气孔的底部或靠近底部的侧壁处进入，再从吸头插入孔的底部或者靠近底部的侧壁处离开，隔离导气孔位于吸头插入孔的上游方向。
2.根据权利要求1所述的用移液器的吸头给亲水性微细管道加入流体样品的加样口，其特征在于：所述吸头插入孔的深度和截面的尺寸或形状足以使该吸头插入到最大深度时不会与吸头插入孔的底面相接触，并使吸头得以固定和稳定。
3.根据权利要求1所述的用移液器的吸头给亲水性微细管道加入流体样品的加样口，其特征在于：所述吸头插入孔截面的周长与面积比小于微细管道截面的周长与面积之比。
CNA2005101307060A 2005-12-23 2005-12-23 用移液器的吸头给亲水性微细管道加入流体样品的加样口 Pending CN1987480A (zh)

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CNA2005101307060A CN1987480A (zh) 2005-12-23 2005-12-23 用移液器的吸头给亲水性微细管道加入流体样品的加样口
EP06828382A EP1963867A4 (en) 2005-12-23 2006-12-18 ANTI-BUBBLE AIR INJECTOR CHAMBER FOR FLUID DEVICES
PCT/CN2006/003471 WO2007071174A1 (en) 2005-12-23 2006-12-18 Bubble-resistant injector port for fluidic devices
US12/096,020 US20090123338A1 (en) 2005-12-23 2006-12-18 Bubble-resistant injector port for fluidic devices

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CNA2005101307060A CN1987480A (zh) 2005-12-23 2005-12-23 用移液器的吸头给亲水性微细管道加入流体样品的加样口

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EP (1) EP1963867A4 (zh)
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WO (1) WO2007071174A1 (zh)

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CN115487879A (zh) * 2021-06-17 2022-12-20 湖南乐准智芯生物科技有限公司 一种减少真空进样气泡的结构及方法、生物芯片

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CN115487879A (zh) * 2021-06-17 2022-12-20 湖南乐准智芯生物科技有限公司 一种减少真空进样气泡的结构及方法、生物芯片
CN115487879B (zh) * 2021-06-17 2024-02-06 湖南乐准智芯生物科技有限公司 一种减少真空进样气泡的结构及方法、生物芯片

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US20090123338A1 (en) 2009-05-14
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