UA65812A - Escherichia coli strain don 910/149 used for preparing vaccine against escherichiosis of cattle - Google Patents

Abstract

Escherichia coli strain Don 910/149 is used for preparing the vaccine against escherichiosis of the cattle.

Description

The invention relates to veterinary microbiology and immunology, in particular to the production of vaccines and antigens.

Ezspepspia soya strain "Don 910/149", which is used for the production of the polyvalent vaccine "Kolisan" and the associated inactivated vaccines "Velshikol", "Velshikolisalm", "Serdosan" against escherichia, salmonellosis, anaerobic enterotoxemia and animal pasteurellosis and "Pneumomastisan" against pneumoenteritis and mastitis of animals, as well as for the production of antigen for immunological reactions.

The purpose of the invention is the EzsPpegisnia soybean strain "Don 910/149". It has well-defined immunogenic properties, which are manifested in the synthesis of specific antibodies in the body of vaccinated animals. The strain was isolated from a piglet with clinical signs of edema.

The peculiarity of the strain is high stability, production of hemolysin, serogroup specificity, high growth potential, which contributes to obtaining a large amount of biomass for the manufacture of the vaccine, which determines its cost-effective production.

The vaccine strain EvzsPegisnia soybean "Don 910/149" was selected in the IVM of the Ukrainian Academy of Sciences and deposited in the collection of microorganisms of the State Scientific Control Institute of Biotechnology and strains of microorganisms under Me149.

The Ezspegispia soybean vaccine strain "Don 910/149" is characterized by the following features:

Morphological features: bacteria EzsPpegisnia soya strain "Don 910/149" in microscopic preparations from daily broth culture, stained according to Gram - Gram-negative thick rods, located singly or in pairs with rounded ends. Dispute does not form. (1, 2, Z|.

Cultural signs: On MPB at 372C rnb, 8-7.2 there is a uniform turbidity of the medium, on MLA it forms rounded, smooth, shiny gray-white colonies (Z-forms). On a differential medium, Endo grows in the form of red colonies with a metallic tint. The color of the CODE environment changes from blue to green 1, 2. 4.

Enzymatic properties: "Don 910/149" Euspegisnpia soybean bacteria actively break down sugar, especially mannitol. It is weaker than glucose, maltose and lactose - with the formation of acid and gas. Forms indole.

Pathogenic properties; pathogenic for white mice with intraperitoneal administration of a daily agar culture suspension in physiological solution, in a volume of 0.5 cm3 and a concentration of 400 mln. microbial cells, which is I 0100.

The death of animals is noted within 5 days.

Immunogenic properties: two-time parenteral vaccination of white mice with an anaculture of this strain in a volume of 0.5 cm3 protects against control infection with a virulent culture of EzsPpeponia soya in 8595 cases. (5)

The invention is illustrated by the following examples.

Example 1. White mice weighing 16-18 g were immunized with an anaculture of EzsNegsnia soya strain "Don 910/149" in the amount of 10 heads subcutaneously and 10 heads intramuscularly in a volume of 0.5 cm3. Vaccination was carried out twice with an interval of 10-12 days. On the 12th day after the second vaccination, mice were infected with a virulent culture of the strain

Ezspegispia soybean "Don 910/149" in a dose of 400 mln. microbial bodies. As a control, non-immunized white mice were used, in the amount of 10 heads.

It was established that the coefficient of immunological efficiency of immunized animals was 8,595 with 10,095 deaths of control animals (Table 1).

Example 2. 15 piglets at the age of 3.5 months were immunized with "Don 910/149" in a volume of 3.0 cm3 twice with an interval of 12 days. As a control for immunization, 10 heads were used subcutaneously in the neck area with a culture of the EzsPegispia soya strain of non-immunized piglets - analogs. Before vaccination, on the 7th and 14th days, blood samples were taken from piglets of both groups and blood serum was examined for the presence of antibodies in the agglutination reaction. Antibody titers to the strain

Ezspetispia soybean "Don 910/149" are presented in Table 2.

Table 1

Results of infection control of white mice inoculated with anaculture strain EzsPetispia soybean "Don 910/149" 000000 M avu y kli t p/p anaculture -- re Z 1 Ol sho ryu 02ЗМ0« | ов - 50-52 5 5 6 о 5 66 68550 2. Strula // /Вн/myazovo,d/ | bom) 10 | 2 | 20 | 8 83. |Strula 1-77 | bb5sme| 10 | 10 | l00 | 0 | at

Table 2

Titers of antibodies in the blood serum of piglets vaccinated with an anaculture of EzsPegispia soya strain "Donoe10/149" immunized control 10 3.0 cm3

As can be seen from Table 2, during immunization with an anaculture of EzsPegisnia soybean strain "Don 910L49", antibody titers in blood serum of piglets of the 3.5-month-old group were 1:320-1:640. So, the obtained results shown in Tables 1 and 2 indicate that immunization of animals with an anaculture of Ezspepspia soya strain "Don 910/149" protects white mice from infection with a virulent culture of this strain and promotes the synthesis of specific antibodies and the formation of immunity in piglets.

The peculiarity of the EuspPegisnia soybean strain "Don 910/149" is the production of hemolysin, the presence of metallic luster of the colonies, it has well-defined morphological, cultural, biochemical, pathogenic properties characteristic of the EzsPepisnia genus.

Conducted studies have shown that the Don 910/149 strain of Euspepspia soybean has a well-expressed immunogenic activity, which makes it possible to recommend it for the production of vaccines against animal escherichia.

Sources of information: 1. Determinant of Berghi bacteria (edited by J. Hout, N. Krita, P. Snyt, etc.). Translation from the English academic.

RAN GA. Zavarzyna 9th edition in 2 volumes-1997-4326b. 2. Veterinary microbiology edited by J.E. Kolyakov, post. scientist of the RSFSR prof. Moscow Veterinary Academy. 3rd edition. Moscow 1965

Z. Golovko A.N., Hnatenko G.V. Fimbrial adhesins of Eusppepspia soya and their role in the pathogenesis of colibacteriosis of farm animals and birds // Prophylaxis and measures to combat diseases of young cattle. Zh.-Kh:

Thesis report / Republican scientific-practical conference - Minsk, 1990 - P.38. 4. Guidelines for laboratory diagnosis of escherichia (colibacteriosis) in animals / A.M. Golovko, V.O. Ushkalov,

P.P. Fuchs and others // Kyiv, 1995-2066. 5. Ryzhenko V.P., Ryzhenko G.F., Akymenko L.I., Ryzhenko I.V., Dementyeva S.A., Belik S.M., Ryzhenko V.V., Galka

I.V., Bezymennyi M.V., Marchenko O.M., Chernikov O.O. Scientific rationale for the development and effectiveness of the use of associated vaccines // Scientific Bulletin of the National Academy of Sciences, 2001. - Mo36b. - P.43-49.

Claims (1)

Strain EzsepPegisnia soya "Don 910/149", deposited in the official collection of strains of microorganisms DNAIBShM under number 149, proposed for the production of polyvalent and associated inactivated vaccines "Kolisan", "Velshikolisalm", "Serdosan", "Pneumomastisan", "Poliavisan" against colibacteriosis of cattle, pigs and poultry and is characterized by stable morphological, cultural, virulent and well-expressed immunological properties.