UA152369U - THE METHOD OF CRYOCONSERVATION OF ERYTHROCYTES - Google Patents
THE METHOD OF CRYOCONSERVATION OF ERYTHROCYTES Download PDFInfo
- Publication number
- UA152369U UA152369U UAU202106223U UAU202106223U UA152369U UA 152369 U UA152369 U UA 152369U UA U202106223 U UAU202106223 U UA U202106223U UA U202106223 U UAU202106223 U UA U202106223U UA 152369 U UA152369 U UA 152369U
- Authority
- UA
- Ukraine
- Prior art keywords
- erythrocytes
- nacl
- erythromass
- washing
- cryoconservation
- Prior art date
Links
- 210000003743 erythrocyte Anatomy 0.000 title abstract 3
- 238000005138 cryopreservation Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 3
- 239000011780 sodium chloride Substances 0.000 abstract 2
- 238000005406 washing Methods 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 abstract 1
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 abstract 1
- 229960004308 acetylcysteine Drugs 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000002338 cryopreservative effect Effects 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 239000000600 sorbitol Substances 0.000 abstract 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Спосіб кріоконсервування еритроцитів, при якому виконують відмивання еритромаси розчином, що містить 0,9 % NaCl, додавання до еритроцитів у співвідношенні 1:1 кріоконсерванту, що містить 38 % гліцерину, 2,9 % сорбітолу, 0,63 % NaCl і воду дистильовану, інкубацію протягом 20 хв. при 25-26 °C, заморожування до -196 °C, відігрівання на водяній бані при 41-45 °C і відмивання клітин від кріоконсерванту гіпертонічним та фізіологічним розчинами NaCl. В розчин для відмивання еритромаси додатково вводять N-ацетилцистеїн в концентрації 0,3 %.The method of cryopreservation of erythrocytes, in which the erythromass is washed with a solution containing 0.9% NaCl, the cryopreservant containing 38% glycerol, 2.9% sorbitol, 0.63% NaCl and distilled water is added to erythrocytes in a ratio of 1:1. incubation for 20 min. at 25-26°C, freezing to -196°C, rewarming in a water bath at 41-45°C, and washing the cells from cryopreservative with hypertonic and physiological NaCl solutions. N-acetylcysteine in a concentration of 0.3% is additionally injected into the solution for washing erythromass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU202106223U UA152369U (en) | 2021-11-05 | 2021-11-05 | THE METHOD OF CRYOCONSERVATION OF ERYTHROCYTES |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU202106223U UA152369U (en) | 2021-11-05 | 2021-11-05 | THE METHOD OF CRYOCONSERVATION OF ERYTHROCYTES |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA152369U true UA152369U (en) | 2023-01-18 |
Family
ID=88731971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UAU202106223U UA152369U (en) | 2021-11-05 | 2021-11-05 | THE METHOD OF CRYOCONSERVATION OF ERYTHROCYTES |
Country Status (1)
| Country | Link |
|---|---|
| UA (1) | UA152369U (en) |
-
2021
- 2021-11-05 UA UAU202106223U patent/UA152369U/en unknown
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