UA122019U - METHOD OF DIAGNOSTICS OF ATHEROSCLEROSIS DEVELOPMENT - Google Patents

Abstract

A method of diagnosing the development of atherosclerosis includes conducting a clinical examination, study of low and high density lipoprotein cholesterol levels, arteriography, determination of MTHFR C677T, eNOS T786C gene polymorphism in the serum, thrombodulin content, folic acid, folic acid. With homozygous carrier 677-TT, 786-CC, thrombomodulin levels> 5 ng / ml, folic acid <6 ng / ml, osteocalcin <21 ng / ml, homocysteine> 20 μmol / l diagnose the development of atherosclerosis.

Description

The proposed method of diagnosing the development of atherosclerosis belongs to medicine, in particular to therapy and rheumatology. It is prescribed and can be used in the treatment and examination of patients.

Ways of diagnosing the development of atherosclerosis are known. These include determination of low- and high-density lipoprotein cholesterol levels, arteriography (Okorokov A.N.

Diagnosis of diseases of internal organs. - T. 6. - M.: Med. lit., 2003. - pp. 75-85).

However, the known method is not effective enough and does not allow diagnosing the development of atherosclerosis even at the onset of the disease. Accordingly, there is no possibility to prevent atherosclerosis.

The basis of a useful model is the task of developing a method that would allow diagnosing the onset of atherosclerosis at an early stage.

The task is solved by the fact that along with the study of low and high-density lipoprotein cholesterol levels, arteriography in the patient's blood serum determines the polymorphism of the genes methylenetetrahydrofolate reductase (MITNEV Сб677Т), nitric oxide synthase (emo5 17860), the content of thrombomodulin, folic acid, osteocalcin, and homocysteine. With homozygous carriers of 677-TT, 786-SS, levels of thrombomodulin "5 ng/ml, folic acid "6 ng/ml, osteocalcin "21 ng/ml, homocysteine 220 μmol/l, the development of atherosclerosis is diagnosed.

Application of the method

During hospitalization, the patient is examined, low- and high-density lipoprotein cholesterol levels are examined, and arteriography is performed. Gene polymorphisms are determined in blood serum

MTNEV S677T, eMmo5 1T786S, immunoenzymatic method - content of thrombomodulin, folic acid, osteocalcin, homocysteine. With homozygous carriers of 677-HT, 786-SS, levels of thrombomodulin "5 ng/ml, folic acid "6 ng/ml, osteocalcin "21 ng/ml, homocysteine "20 μmol/l, the development of atherosclerosis is diagnosed.

A specific example of using the method

Patient M., 45 years old, was hospitalized with a diagnosis of rheumatoid arthritis. Inspected.

Low and high density lipoprotein cholesterol levels were studied. An arteriography of vessels was performed. There are no signs of atherosclerosis. Polymorphism of MTNEV genes was determined in blood serum

From C677T and emMmo5 T7860. The content of thrombomodulin, folic acid, osteocalcin, homocysteine was determined by immunoenzymatic method. Homozygous carriers of 677-177 and 786-SS were established.

Levels of thrombomodulin - 11 ng/ml, folic acid - 3.8 ng/ml, osteocalcin - 13 ng/ml, homocysteine - 26 μmol/l. The development of atherosclerosis was diagnosed. Arteriographic signs of the latter were detected only after 13 months.

Thus, the proposed method allows diagnosing atherosclerosis in the early stages.

Claims (1)

USEFUL MODEL FORMULA 40 The method of diagnosing the development of atherosclerosis, which includes conducting a clinical examination, researching the levels of low and high-density lipoprotein cholesterol, arteriography, which is distinguished by the fact that the polymorphism of the MTNEV C677tT, emo5 T786C genes, the content of thrombomodulin, folic acid, osteocalcin, homocysteine, are determined in the blood serum. and with homozygous carrier 677-TT, 786-SS, levels of thrombomodulin "5 ng/ml, folic acid "6 45 ng/ml, osteocalcin "21 ng/ml, homocysteine 220 μmol/l diagnose the development of atherosclerosis.