UA121658U - METHOD OF DIAGNOSTICS OF ATHEROSCLEROSIS DEVELOPMENT - Google Patents

Abstract

A method of diagnosing the development of atherosclerosis includes clinical examination, studies of low and high density lipoprotein cholesterol levels, arteriography, determination of MTHFR C677T, eNOS T786C gene polymorphism, thrombomodulin content, folic acid, folic acid, thrombomodulin, folic acid. In the homozygous carrier 677-TT, 786-CC, thrombomodulin levels> 5 ng / ml, folic acid <6 ng / ml, pyridinoline> 8 ng / ml, IL-6> 10 ng / l, homocysteine> 20 μmol / l are diagnosed development of atherosclerosis.

Description

The proposed method of diagnosing the development of atherosclerosis belongs to medicine, in particular to therapy and rheumatology. It is prescribed and can be used in the treatment and examination of patients.

Ways of diagnosing the development of atherosclerosis are known. These include determination of cholesterol levels of low and high density lipoproteins, arteriography of I.N. Okorokov. Diagnosis of diseases of internal organs. - T. 6-S. 75-85.

However, the known method is not effective enough and does not allow diagnosing the development of atherosclerosis even at the onset of the disease. Accordingly, there is no possibility to prevent atherosclerosis.

The basis of a useful model is the task of developing a method that would allow diagnosing the onset of atherosclerosis at an early stage.

The task is solved by the fact that along with the study of low- and high-density lipoprotein cholesterol levels, arteriography in the patient's blood serum determines the polymorphism of the genes methylenetetrahydrofolate reductase (MINEV Сб677Т), nitric oxide synthase (emo5 1786С), the content of thrombomodulin, folic acid, pyridinoline, interleukin b ( IL-b), homocysteine. With homozygous carriers of 677-HT, 786-SS, levels of thrombomodulin "5 ng/ml, folic acid "6 ng/ml, pyridinoline 28 ng/ml, IL-b6 "10 ng/l, homocysteine 220 μmol/l, the development of atherosclerosis is diagnosed .

Application of the method. During hospitalization, the patient is examined, low- and high-density lipoprotein cholesterol levels are examined, and arteriography is performed. Polymorphism of genes MTNEV 677, emMmO5 1786C, thrombomodulin, folic acid, pyridinoline, IL-b6, homocysteine content is determined in blood serum by immunoenzymatic method. With homozygous carriers of 677-HT, 786-SS, levels of thrombomodulin "5 ng/ml, folic acid "6 ng/ml, pyridinoline "8 ng/ml, IL-b6 "10 ng/l, homocysteine 220 μmol/l, the development is diagnosed atherosclerosis.

A specific example of using the method.

Patient M, 46 years old, was hospitalized with a diagnosis of rheumatoid arthritis. Inspected.

Low and high density lipoprotein cholesterol levels were studied. An arteriography of vessels was performed. There are no signs of atherosclerosis. Polymorphism of MTNEV genes was determined in blood serum

From C677T and emMmo5 T7860. The content of thrombomodulin, folic acid, pyridinoline, IL-6, and homocysteine was determined by immunoenzymatic method. Homozygous carriers of 677-1T and 786-

SS. Levels of thrombomodulin - 10.8 ng/ml, folic acid - 3.8 ng/ml, pyridinoline - 19 ng/ml, IL-6 - 15 ng/l, homocysteine - 26 μmol/l. The development of atherosclerosis was diagnosed. Arteriographic signs of the latter were detected only after 14 months.

Thus, the proposed method allows diagnosing atherosclerosis in the early stages.

Claims (1)

USEFUL MODEL FORMULA 40 The method of diagnosing the development of atherosclerosis, which includes a clinical examination, a study of low and high-density lipoprotein cholesterol levels, arteriography, determination of the polymorphism of the MITNEV C677T, eMmO5 T786C genes in blood serum, the content of thrombomodulin, folic acid, pyridinoline, IL-6, homocysteine, which differs the fact that with homozygous carrier 677-HT, 786-SS, levels of thrombomodulin "5 ng/ml, folic acid "6 ng/ml, 45 pyridinoline »8 ng/ml, IL-b »10 ng/l, homocysteine 220 μmol/l diagnose the development of atherosclerosis.