UA121119U - METHOD OF DIAGNOSTICS OF ATHEROSCLEROSIS DEVELOPMENT - Google Patents

Abstract

A method of diagnosing the development of atherosclerosis includes clinical examination, studies of low and high density lipoprotein cholesterol levels, arteriography, determination of eNOS T786C gene polymorphism in the serum, thrombomodulin content, GAG, IL-6, homocysteine. With homozygous carrier 786-CC, thrombomodulin levels> 5 ng / ml, GAG> 28 μmol / l, IL-6> 10 ng / l, homocysteine> 20 μmol / l diagnose the development of atherosclerosis.

Description

The proposed method of diagnosing the development of atherosclerosis belongs to medicine, in particular to therapy and rheumatology. It is prescribed and can be used in the treatment and examination of patients.

Ways of diagnosing the development of atherosclerosis are known. These include determination of cholesterol levels of low and high density lipoproteins, arteriography (Okorokov A.N. Diagnostics of diseases of internal organs. - M.: Med. lit., 2003. - Vol. 6. - P. 75-85).

However, the known method is not effective enough and does not allow diagnosing the development of atherosclerosis even at the onset of the disease. Accordingly, there is no possibility to prevent atherosclerosis.

The basis of a useful model is the task of developing a method that would allow diagnosing the onset of atherosclerosis at an early stage.

The task is solved by the fact that along with the study of low and high density lipoprotein cholesterol levels, the polymorphism of the nitric oxide synthase gene (eMO5 T7860C), the content of thrombomodulin, glycosaminoglycans (GAG), interleukin 6 (IL-6), homocysteine is determined in the patient's blood serum by arteriography . With homozygous carrier 786-

SS, levels of thrombomodulin »5 ng/ml, HAG »28 μmol/l, IL-b6 »10 ng/l, homocysteine 220 μmol/l diagnose the development of atherosclerosis.

Application of the method. During hospitalization, the patient is examined, low- and high-density lipoprotein cholesterol levels are examined, and arteriography is performed. In the patient's blood serum, the polymorphism of the eMO5 1786C gene is determined, the content of thrombomodulin is determined by the immunoenzymatic method,

GAG, IL-6, homocysteine. With homozygous carrier 786-SS, thrombomodulin levels »5 ng/ml,

GAG 528 μmol/l, IL-b6 210 ng/l, homocysteine 220 μmol/l diagnose the development of atherosclerosis.

A specific example of using the method

Patient B., 46 years old, was hospitalized in the clinic with a diagnosis of rheumatoid arthritis. Examined

Low and high density lipoprotein cholesterol levels were studied. Arteriography was performed.

There are no signs of atherosclerosis. Polymorphism of the eMO5 T7860 gene was determined in blood serum.

The content of thrombomodulin, GAG, IL-b, and homocysteine was determined by immunoenzymatic method.

Homozygous carrier 786-SS was established. Thrombomodulin levels - 13 ng/ml, GAG - 38 μmol/l,

IL-6-19 ng/l, homocysteine - 34 μmol/l. The development of atherosclerosis was diagnosed.

Arteriographic signs of the process were revealed only after 12 months.

Thus, the proposed method of diagnosis allows establishing a diagnosis at the early stages of the development of the process.

Claims (1)

USEFUL MODEL FORMULA A method of diagnosing the development of atherosclerosis, which includes a clinical examination, low- and high-density lipoprotein cholesterol levels, arteriography, determination of the eMO5 T786C gene polymorphism, the content of thrombomodulin, GAG, IL-6, and homocysteine in blood serum, which is distinguished by the fact that homozygous carrier 786-SS, levels of thrombomodulin 25 ng/ml, GAG 528 μmol/l, IL-b6 210 ng/l, homocysteine 220 μmol/l are diagnosed with the development of atherosclerosis.