TWM609297U - Composite micro particle structure of filtrate of film-coated fermented yeast extract to promoting keratin renewal - Google Patents

Abstract

This creation is about a composite particle structure of the fermentation extract filtrate of Saccharomycopsis that can promote keratinogenesis, especially a functional essence layer containing the fermentation extract filtrate of the coated yeast sequentially from the inside to the outside , And a composite particle structure of a structural protective layer. The aforementioned composite particle structure can prevent the coated yeast fermentation extract filtrate from being affected by air, light or microorganisms before use, reduce the loss of active ingredients, and improve skin keratinocyte regeneration and promotion Collagen grows, enhances the antibacterial effect of sebum outside the stratum corneum, exfoliates and maintains fine and smooth skin, inhibits the generation of free radicals, and maintains skin elasticity.

Description

A composite particle structure of the filtrate of the fermented yeast extract from the film to promote keratin renewal

This creation is about a composite particle structure of the fermented extract filtrate of Sacch-aromycopsis that can promote keratinogenesis, especially a kind of filtrate containing the fermented extract of Sacch-aromycopsis sequentially from the inside to the outside The functional essence layer and the composite particle structure of a structural protective layer.

According to, modern people live in high-stress and high-polluting environments for a long time, such as UV exposure, irregular diet and work and rest, or in the process of metabolism, free radicals are generated and attack the skin tissues, making the skin prone to dullness, spots, and fine lines. , Dryness, loss of elasticity and other problems. In addition, due to increasingly serious air pollution, many people suffer from skin allergies and inflammation due to suspended particles in the air and irritating chemicals produced by industrialization. In such an environment, the skin is prone to dryness, peeling, and itching. And peeling and other issues. How to help the skin tissue resist the attacks of external and internal adverse factors, ease the continuous damage of the skin, and at the same time improve the skin's self-repairing ability, has become an important topic in the design of skin care products today.

Because of its unique physiological characteristics, yeast has been widely used in the food industry in the past or in the current biotechnology field, and the fermentation of alcohol is particularly the bulk of it. According to legend, the skin of the workers who handle the fermentation of sake in the Japanese sake workshops is always exceptionally smooth, white, and elastic. Even in middle-aged and elderly people, the skin on all parts of the body is always extraordinarily smooth, fair and elastic. The skin of the palms of one hand is still smooth and tender after aging begins. Japanese researchers believe that the workers were exposed to a large number of yeast fermentation broths during the fermentation process of sake, and they eventually separated differently. Yeast strains that can improve skin keratin renewal were discovered later.

Coated yeast, also known as endosporum saccharomyces, belongs to the branch of fragrant yeast. It is mostly displayed in the form of dominant bacteria in the process of making fragrant liquor, rice wine, rice wine and other brewing wines. During the fermentation process, the coated yeast can not only produce high-grade alcohols, esters and other aromatic fragrance substances, but also has more abundant secondary metabolites than ordinary yeasts, because a large amount of secondary metabolites are secreted during the fermentation process. The acidic protein plum, β-glucosidase, amylase and other enzymes, the coated yeast can fully consume the nutrients in the medium during the fermentation process, decompose large molecular proteins, and produce abundant small molecular organic acids. , Peptides, vitamins, and large amounts of oligosaccharides, such as trehalose, which have excellent moisturizing and moisturizing effects on the skin. Except for a small part of these small molecules that are absorbed by the yeast itself, most of them remain in the fermentation broth. After filtering and purifying the fermentation broth, the filtrate of the coated yeast can be obtained.

The filtrate of this coated yeast is between pH 6.0 and 8.0, which can enhance the antibacterial effect of the outer sebum of the stratum corneum, exfoliate and maintain skin smoothness, inhibit the generation of free radicals, protect skin cells from damage, and enhance skin anti-aging Ability. At the same time, it can maintain the water content of the stratum corneum, promote the renewal of the stratum corneum and the growth of subcutaneous collagen, thereby maintaining the elasticity of the skin, making the skin hydrated and white. For oily skin, the coated yeast filtrate can control sebum secretion and condition the skin. The sebum secretion balances and promotes sebum from the pores to the skin surface, thereby inhibiting the formation of acne. However, this ingredient is easily damaged by sunlight and oxidation, so there are still many inconveniences in use.

The reason is that, in view of this, the creators have worked tirelessly to solve the above problems, and with the help of his wealth of professional knowledge and years of practical experience, he has created a Saccharomycopsis that can promote keratin renewal. ) The composite particle structure of the fermented extract filtrate can help change the rough and aged keratin structure, promote the renewal of the keratin and stimulate the growth of subcutaneous collagen, and then achieve the effect of restoring the skin's luster, white and tenderness. It can be applied to a variety of In the form of skin care products.

The present invention provides a composite particle structure of Saccharomycopsis fermentation extract filtrate, which can promote keratinogenesis. The structure from the inside to the outside includes: a functional essence layer containing the fermentation extract filtrate of the coated yeast; and Structural protection layer, which covers the functional essence layer.

In an embodiment of the present invention, the composite particle structure containing the fermented filtrate of the coated yeast which can promote keratinogenesis, the particle size of the composite particle structure is 150 to 500 microns.

In another embodiment of the present invention, the thickness of the functional essence layer in the composite particle structure containing the fermented yeast extract filtrate of the film-coated yeast that can promote keratinogenesis is 60 micrometers to 250 micrometers.

In another embodiment of the present invention, the thickness of the protective layer of the structure in the composite particulate structure containing the fermented yeast extract filtrate of the film-coated yeast that can promote keratinogenesis is 90 micrometers to 250 micrometers.

In yet another embodiment of the present invention, the composite particle structure containing the fermented filtrate of the coated yeast that can promote keratin regeneration, and the functional essence layer is in a homogeneous state.

In yet another embodiment of the present invention, the composite particle structure containing the membrane-coated yeast fermentation extract filtrate that can promote keratinogenesis is formed, and the protective layer in the composite particle structure is shaped Agent layer.

In yet another implementation aspect of the present invention, the composite particle structure containing the fermented filtrate of the coated yeast that can promote keratinogenesis can be in the form of powder, solution, suspension, gel, emulsion, or emulsion. Cream-like or mask-like form.

One of the effects of this new creation is that by providing a composite particle structure containing the membrane-coated yeast fermentation extract filtrate that can promote keratin renewal, the functional essence layer containing the effective ingredients of the membrane-coated yeast fermentation extract filtrate is wrapped in the structural protective layer in. The aforementioned composite particle structure can not only avoid the influence of air or light or microorganisms on the fermented yeast extract filtrate of the coated yeast before use, reduce the loss of active ingredients, but also help resist skin tissues when applied to the skin. High-stress and high-polluting environments, such as UV exposure, irregular diet and work and rest, or the metabolism process, generate free radicals and attack the skin tissues, making the skin prone to dullness, spots, fine lines, dryness, loss of elasticity and other problems. In addition, the composite particle structure of this creation can also increase the regeneration of skin keratinocytes and promote the growth of collagen, enhance the antibacterial effect of the stratum corneum sebum, exfoliate and maintain skin smoothness, and inhibit the generation of free radicals, thereby maintaining skin elasticity and allowing The skin is hydrated and white, for oily skin, it can regulate the skin's oil secretion balance, and at the same time promote sebum from the pores to the skin surface, thereby inhibiting the formation of acne.

1: Composite particle structure

11: Functional essence

12: Structural protection layer

A: Functional essence layer thickness

B: Thickness of structural protection layer

Figure 1 is a schematic cross-sectional view of a composite particle structure of a fermented extract filtrate of coated yeast that can promote keratinogenesis according to the present invention.

Figure 2 is the result of the DPPH radical scavenging experiment of the composite particle structure of the fermented extract filtrate of the coated yeast that can promote keratinogenesis according to the new creation.

Figure 3 is a coated yeast that can promote keratinogenesis based on the new creation The results of experiments on the inhibition of tyrosine activity of the composite particle structure of the fermentation extract filtrate.

Figure 4 is a photo of the results of the experiment before and after use of the composite particle structure of the fermentation extract filtrate of the coated yeast that can promote keratinogenesis according to the present invention.

The following will be described with reference to the attached drawings to more fully describe part of the implementation of the new creation; however, the new creation can still be practiced in many different forms and should not be construed as being limited to the implementation exemplified in the specification kind. In addition, in the accompanying drawings, the size of each object and area may be exaggerated for clarity, instead of drawing according to actual scale. At the same time, unless otherwise stated in the text, the "a", "the" and similar terms used in this creation specification (especially in the scope of the patent application described later) should be understood to include singular and plural forms.

Referring to Fig. 1, there is shown a partial cross-sectional schematic diagram of the composite particle structure containing the fermented filtrate of the coated yeast that can promote keratinogenesis according to the present invention. As shown in Figure 1, the new creative composite particle structure 1 includes from the inside out: a functional essence layer 11, which contains the membrane-coated yeast fermentation extract filtrate that can promote keratin regeneration; and a structural protective layer 12 , Which covers the functional essence layer 11 inside.

The composite particle structure 1 of the fermentation extract filtrate of the coated yeast that can promote keratinogenesis of the present invention can be spherical, ellipsoidal, peanut-shaped, or any shape known to those skilled in the art, and does not need to be specifically limited; The term "particle size" refers to the length of the longest axis in the particle structure. The particle size of the particle structure of the present invention can be 150 micrometers to 500 micrometers, preferably 280 micrometers to 450 micrometers. The thickness A of the functional essence layer 11 is usually 60 micrometers to 250 micrometers, preferably 80 micrometers to 160 micrometers, more preferably 120 micrometers to 240 micrometers; the thickness B of the structural protection layer 12 is usually 90 micrometers to 250 micrometers , Preferably 160 micrometers to 320 micrometers, more preferably 90 micrometers to 120 micrometers. this The particle size of the composite particle structure 1, the thickness of the functional essence layer 1 and the structural protection layer 12 of the new creation of the film-coated yeast fermentation extract filtrate that can promote keratin regeneration can also be observed by those skilled in the art. After obtaining the contents of the description of this case, adjust it as necessary without departing from the scope of this creation;

The functional essence layer 11 of the novel creative composite particle structure 1 can be in a homogeneous state, and its actual composition and content can be adjusted according to needs by those skilled in the art. Except for the membrane-coated yeast fermentation extract filtrate, it can be achieved as desired. The effect of using one or more other effective ingredients, and adjust the ratio between the effective ingredients. In addition, those skilled in the art should adjust the concentration of the active ingredient in the functional essence layer 11 according to the physical and chemical properties of the selected active ingredient, and adjust the amount and ratio of other ingredients to make the functional essence. The essence layer 11 maintains stability and does not deteriorate easily. The other active ingredients and other components in the functional essence layer 11 are not limited to their sources and types, as long as they are compatible with the composite particle structure of the film-coated yeast fermentation extract filtrate that can promote keratin renewal. be usable.

In the composite particulate structure 1 of the present invention, the structural protective layer 12 can also be an excipient. The excipient may include at least one selected from the following groups: fragrances, preservatives, antibacterial agents, chelating agents, penetrants, lubricants, disintegrating agents, emulsifiers, hygroscopic agents, coloring agents, binders, solvents, Diluents, stabilizers, antioxidants, absorption delay agents or combinations of the above. The ingredients actually used in the structural protective layer 12 do not limit their sources, types, and amounts, and can be adjusted as needed by those skilled in the art after viewing the contents of the specification of this case without departing from the scope of this creation.

Example 1: In the following examples, the novel creation of the membrane-coated yeast fermentation extract filtrate that can promote keratinogenesis is used in the DPPH free radical scavenging activity test, and 1000μL DPPH(1,1-Diphenyl2-picryl-hydrazyl) ) Ethanol solution (0.1mM), 450μL Tris-HCl buffer solution (50mM, pH 7.4) and 50μL of test samples of different concentrations were uniformly mixed and then allowed to stand in the dark for 30min. Then, the absorbance at 517nm was measured with an ultraviolet/visible light spectrometer. When the DPPH free radicals are eliminated more, the absorbance will decrease more. Using the percentage reduction of the absorbance relative to the control group, we can know the ability of each test sample to eliminate DPPH free radicals. The strength of the DPPH free radical inhibition rate can represent the strength of the test sample's ability to provide hydrogen (Hydrogen donor) to donate free radicals. DPPH free radical inhibition rate (%)=(1-(absorption value of the experimental group/absorption value of the control group)×100, the results confirmed that the membrane-coated yeast fermentation filtrate has nearly 90% high free radical scavenging ability.

Example 2: In the following examples, the results of the experiment of using the membrane-coated yeast fermentation extract filtrate to inhibit tyrosinase activity, which can promote the keratinogenesis of the new creation, are used in different concentrations (1%, 2.5%, 5%) of the coated yeast fermentation filtrate samples and 4-Butylresorcinol (4-Butylresorcinol) were tested for mushroom tyrosianse activity. Among them, different concentrations of butylresorcinol were used as control standards Product. In 96 well plates (96-well multiplate) in 20 μ L samples were taken disposed added 25 μ L of tyrosinase (50unit) mixed, allowed to stand at room temperature for 10 minutes to spectrophotometer absorbance of 492nm As the background value, then 155 μl , 2.5mM L-Dopa (L-Dopa) was added to a 96-well plate, and the absorbance of the reaction mixture in each well at 492nm was measured with a spectrophotometer (BioTek, SynergyTM2, USA) The value changes. Its tyrosinase activity inhibition rate (%) calculation formula is [1-(absorbance value of sample at 492nm/absorbance value of control group without sample added at 492nm)]×100%. Please refer to Figure 3 for the results. Under the action of different concentrations of 1%, 2.5%, and 5% of the fermentation filtrate samples of the coated yeast, the inhibition of mushroom tyrosinase activity (inhibition of mushroom tyrosinase activity) increased with the increase of the working concentration. Promote. The results confirmed that the membrane-coated yeast fermentation filtrate has the effect of inhibiting mushroom tyrosinase activity in vitro.

Example 3: In the following examples, the results of a clinical trial of the skin-coated yeast fermentation extract filtrate that can promote keratinogenesis of the new creation are presented. The test was conducted by a volunteer and used the coated yeast to ferment The filtrate was sampled by microscopy before the test. During the 28-day test, the original daily life and the same facial cleansing behavior were maintained. The only difference was that a sample containing 5% of the coated yeast fermentation filtrate was applied to the face before going to bed. After applying evenly on the body, let it dry naturally. After 28 days, it was sampled again under the same conditions for microscopic examination. The result showed that the volunteer's facial skin sample changed from the original dullness to tenderness and lightness. White.

1: Composite particle structure

11: Functional essence

12: Structural protection layer

A: Functional essence layer thickness

B: Thickness of structural protection layer

Claims (7)

A composite particle structure of a film-coated yeast fermentation extract filtrate that can promote keratinogenesis, which includes from the inside to the outside: a functional essence layer containing the film-coated yeast fermentation extract filtrate; and a structural protection layer, which The functional essence layer is covered. The composite particle structure of the fermentation extract filtrate of the coated yeast that can promote keratinogenesis as described in claim 1 has a particle size of 150 micrometers to 500 micrometers. The composite particle structure of the membrane-coated yeast fermentation extract filtrate that can promote keratinogenesis as described in claim 1, wherein the functional essence layer has a thickness of 60 micrometers to 250 micrometers. The composite particle structure of the membrane-coated yeast fermentation extract filtrate that can promote keratinogenesis as described in claim 1, wherein the protective layer of the structure has a thickness of 90 micrometers to 250 micrometers. The composite particle structure of the fermented yeast extract filtrate of the coated yeast that can promote keratinogenesis as described in claim 1, wherein the functional essence layer is in a homogeneous state. The composite particle structure of the fermentation extract filtrate of the coated yeast that can promote keratinogenesis as described in claim 1, wherein the protective layer of the structure is an excipient. The composite particle structure of the fermented yeast extract filtrate that can promote keratinogenesis as described in claim 1. The composite particle structure can be made into powder, solution, suspension, gel, emulsion, cream , Or in the form of a mask.

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