CN110279597A - Rhizobium fermentation composition used for cosmetic with anti-inflammatory moisture-keeping efficacy, preparation method and applications - Google Patents
Rhizobium fermentation composition used for cosmetic with anti-inflammatory moisture-keeping efficacy, preparation method and applications Download PDFInfo
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- CN110279597A CN110279597A CN201910606802.XA CN201910606802A CN110279597A CN 110279597 A CN110279597 A CN 110279597A CN 201910606802 A CN201910606802 A CN 201910606802A CN 110279597 A CN110279597 A CN 110279597A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The present invention relates to cosmetic technical fields, and in particular to rhizobium fermentation composition used for cosmetic, preparation method and applications with anti-inflammatory moisture-keeping efficacy.The invention discloses a kind of moisture-keeping efficacy compositions, it is characterised in that forms Arbutin, vitamin E, glycerol, butanediol, pulullan polysaccharide, hyaluronic acid, root nodule granulose, ophiopogonpolysaccharide, aloe polysaccharide, allantoin, Radix Astragali by following raw materials according.Moisturizer provided by the invention not only has good replenishing water and preserving moisture effect, and has certain antiinflammation.
Description
Technical field
The invention belongs to cosmetic skin fields, and in particular to the rhizobium used for cosmetic with anti-inflammatory moisture-keeping efficacy are fermented
Composition, preparation method and applications.
Background technique
The rapid development and extensive use of modern science and technology bring completely new opportunity to develop to cosmetic industry, change
Cosmetic is from the basic skin care item for the purpose of clean skin, moisturizing to type cosmetics side the effect of delaying senescence, beautify the complexion as purpose
To development, the research and development of cosmetics have been subjected to chemical beauty, plant beauty, just stride forward towards biological beauty.
Vitamin E can effectively antagonize free radical, inhibit lipid peroxide to generate, eliminate chloasma;Inhibit tyrosinase
Activity, to reduce melanin production.The vitamin E of esterified form can also be eliminated by extraneous factors such as ultraviolet light, air pollutions
Caused by excessive oxygen radical, the effects of playing anti-aging, prevention sunburn.
Radix Ophiopogonis is one of Chinese tradition Chinese medicine, has a variety of medicinal efficacies.Main chemical component has polysaccharide, steroid in Radix Ophiopogonis
Body saponin(e, homoisoflavone class etc. have a variety of pharmacological activities, and such as antiallergy is anti-oxidant etc., and ophiopogonpolysaccharide is in Radix Ophiopogonis
One of main effective medicinal ingredient.
Radix Astragali is one of China's Chinese medicine used for a long time, has the function of enhancing immune, anti-aging and anti-inflammatory.
Rhizobia exocellular polysaccharide has good water solubility, has good filming performance, can form one in skin surface
The uniform film of layer reduces the moisture evaporation of skin surface so that moisture permeates into cuticula from substrate microstructure, induces angle
The further aquation of matter layer, saves the moisture of skin itself, and completes moisturizing effect, and high water absorbing ability and good film forming are complete
It beauteously combines, good moistening effect can be provided for skin, and viscosity is low, good surface activity, be a kind of potential moisturizer.
Summary of the invention
The purpose of the present invention is to provide the rhizobium fermentation compositions used for cosmetic with anti-inflammatory moisture-keeping efficacy, preparation side
Method and its application, the anti-inflammatory moisture-keeping composition is by following parts by weight at being grouped as: 4-8 parts of Arbutin, vitamin E 5-
10 parts, 10-20 parts of glycerol, 1-2 parts of butanediol, 1-3 parts of pulullan polysaccharide, 2-4 parts of hyaluronic acid, rhizobia exocellular polysaccharide 10-
30 parts, 2-5 parts of aloe polysaccharide, 1-3 parts of ophiopogonpolysaccharide, 2-8 parts of allantoin, 0.5-1.5 parts of Radix Astragali, 30-60 parts of water;
Preferably, the composition composition is as follows: 4 parts of Arbutin, 8 parts of vitamin E, 15 parts of glycerol, 1 part of butanediol, Propiram
3 parts of polysaccharide, 3 parts of hyaluronic acid, 20 parts of rhizobia exocellular polysaccharide, 5 parts of aloe polysaccharide, 3 parts of ophiopogonpolysaccharide, 5 parts of allantoin, Radix Astragali
0.5 part, 50 parts of water;
The hyaluronic acid includes three kinds of molecular weight, and respectively 18-22 ten thousand, 10-18 ten thousand, 1-4 ten thousand, proportion is 1:1:1;
The pulullan polysaccharide molecular weight is 10-30 ten thousand;
The rhizobia exocellular polysaccharide the preparation method is as follows:
To by maltose 20-30g/L, soy peptone 7-10 g/L, MgSO4•7H2O 0.1-0.3g/L, KH2PO4 0.2-
The rhizobium of 3-7% are accessed in the sterilising medium of 0.5g/L, NaCl 0.1-0.3g/L composition, 28-32 DEG C, 150-180rpm is trained
70-80h is supported, obtains root nodule fermented liquid, adjusts pH to neutrality, filtration sterilization, alcohol precipitation, freeze-drying obtains rhizobia exocellular polysaccharide.
The present invention also provides the preparation methods of above-mentioned composition, include the following steps
(1) the good Radix Astragali of shape is selected, drying is cleaned, crosses No. six sieves, it is spare;
(2) successively by Arbutin, pulullan polysaccharide, hyaluronic acid, urea, rhizobia exocellular polysaccharide, aloe polysaccharide, ophiopogonpolysaccharide
It is added to the water, low-temperature heat is stirred evenly to 35-45 DEG C, is down to room temperature, obtains mixed liquor A;
(3) glycerol, butanediol are added in mixed liquor A, is uniformly mixed, obtains mixed liquid B;
(4) vitamin E is added in mixed liquid B to get anti-inflammatory moisture-keeping composition.
Preferably, stirring rate is 1000rpm in step (2).
The utility model has the advantages that the composition contains multiple efficacies ingredient, such as vitamin E, root nodule granulose, aloe polysaccharide, Radix Ophiopogonis
Polysaccharide can be effectively improved skin, make skin-tightening, flexible, reduction facial pigmentation deposition, acne-removing inflammation-diminishing, and moisturizing is protected
It is wet, and to skin safe, nonirritant.
Specific embodiment:
In order to which the objects, technical solutions and advantages of this patent are more clearly understood, below in conjunction with specific embodiment, to this patent
It is further elaborated.It should be appreciated that specific embodiment described herein is only used to explain this patent, but it is not used to
It limits the scope of the invention.
The bacterial strain that the present invention uses, will be with rhizobium CICC 10579, the strain of selection in following embodiment for rhizobium
It is interpreted as illustrative and non-specific restriction, the method for the invention is suitable for the production bacterial strain for the same kind being currently known
With.
Embodiment 1 has the rhizobium fermentation composition used for cosmetic of anti-inflammatory moisture-keeping efficacy, preparation method
4 parts of Arbutin, 8 parts of vitamin E, 15 parts of glycerol, 1 part of butanediol, 3 parts of pulullan polysaccharide, 3 parts of hyaluronic acid, rhizobium
20 parts of exocellular polysaccharide, 5 parts of aloe polysaccharide, 3 parts of ophiopogonpolysaccharide, 5 parts of allantoin, 0.5 part of Radix Astragali, 50 parts of water;Specific step is as follows:
(1) the good Radix Astragali of shape is selected, drying is cleaned, crosses No. six sieves, it is spare;
(2) successively by Arbutin, pulullan polysaccharide, hyaluronic acid, urea, rhizobia exocellular polysaccharide, aloe polysaccharide, ophiopogonpolysaccharide
It is added to the water, low-temperature heat is stirred evenly to 35-45 DEG C, is down to room temperature, obtains mixed liquor A;
(3) glycerol, butanediol are added in mixed liquor A, is uniformly mixed, obtains mixed liquid B;
(4) vitamin E is added in mixed liquid B to get anti-inflammatory moisture-keeping composition.
Preferably, stirring rate is 1000rpm in step (2).
Wherein, the rhizobia exocellular polysaccharide the preparation method comprises the following steps: to maltose 20g/L, 8 g/L of soy peptone,
MgSO4•7H2O 0.3g/L, KH2PO4The rhizobium of access 3% in the sterilising medium of 0.41 g/L, NaCl 0.1g/L, 28 DEG C
180rpm cultivates 72h, obtains root nodule fermented liquid, adjusts PH to neutrality, filtration sterilization, alcohol precipitation, and it is extracellular to obtain rhizobium for freeze-drying
Polysaccharide.
Embodiment 2 has the rhizobium fermentation composition used for cosmetic of anti-inflammatory moisture-keeping efficacy, preparation method
4 parts of Arbutin, 8 parts of vitamin E, 15 parts of glycerol, 1 part of butanediol, 3 parts of pulullan polysaccharide, 3 parts of hyaluronic acid, rhizobium
20 parts of exocellular polysaccharide, 3 parts of aloe polysaccharide, 2 parts of ophiopogonpolysaccharide, 5 parts of allantoin, 0.5 part of Radix Astragali, 50 parts of water;Specific step is as follows:
(1) the good Radix Astragali of shape is selected, drying is cleaned, crosses No. six sieves, it is spare;
(2) successively by Arbutin, pulullan polysaccharide, hyaluronic acid, urea, rhizobia exocellular polysaccharide, aloe polysaccharide, ophiopogonpolysaccharide
It is added to the water, low-temperature heat is stirred evenly to 35-45 DEG C, is down to room temperature, obtains mixed liquor A;
(3) glycerol, butanediol are added in mixed liquor A, is uniformly mixed, obtains mixed liquid B;
(5) vitamin E is added in mixed liquid B to get anti-inflammatory moisture-keeping composition.
Preferably, stirring rate is 1000rpm in step (2).
Wherein, the rhizobia exocellular polysaccharide the preparation method comprises the following steps: to maltose 20g/L, 8 g/L of soy peptone,
MgSO4•7H2O 0.3g/L, KH2PO4The rhizobium of access 3% in the sterilising medium of 0.41 g/L, NaCl 0.1g/L, 28 DEG C
180rpm cultivates 72h, obtains root nodule fermented liquid, adjusts pH to neutrality, filtration sterilization, alcohol precipitation, and it is extracellular to obtain rhizobium for freeze-drying
Polysaccharide.
Embodiment 3 has the rhizobium fermentation composition used for cosmetic of anti-inflammatory moisture-keeping efficacy, preparation method
4 parts of Arbutin, 8 parts of vitamin E, 15 parts of glycerol, 1 part of butanediol, 3 parts of pulullan polysaccharide, 3 parts of hyaluronic acid, rhizobium
20 parts of exocellular polysaccharide, 2 parts of aloe polysaccharide, 3 parts of ophiopogonpolysaccharide, 5 parts of allantoin, 0.5 part of Radix Astragali, 50 parts of water;Specific step is as follows:
(1) the good Radix Astragali of shape is selected, drying is cleaned, crosses No. six sieves, it is spare;
(2) successively by Arbutin, pulullan polysaccharide, hyaluronic acid, urea, rhizobia exocellular polysaccharide, aloe polysaccharide, ophiopogonpolysaccharide
It is added to the water, low-temperature heat is stirred evenly to 35-45 DEG C, is down to room temperature, obtains mixed liquor A;
(3) glycerol, butanediol are added in mixed liquor A, is uniformly mixed, obtains mixed liquid B;
(4) vitamin E is added in mixed liquid B to get anti-inflammatory moisture-keeping composition.
Preferably, stirring rate is 1000rpm in step (2).
Wherein, the rhizobia exocellular polysaccharide the preparation method comprises the following steps: to maltose 20g/L, 8 g/L of soy peptone,
MgSO47H2O 0.3g/L, KH2PO4The rhizobium of access 3% in the sterilising medium of 0.41 g/L, NaCl 0.1g/L, 28 DEG C
180rpm cultivates 72h, obtains root nodule fermented liquid, adjusts PH to neutrality, filtration sterilization, alcohol precipitation, and it is extracellular to obtain rhizobium for freeze-drying
Polysaccharide.
Comparative example 1:
A kind of anti-inflammatory moisturizer, the anti-inflammatory moisturizer prepared with embodiment 1 is not the difference is that contain aloe polysaccharide.
Comparative example 2:
A kind of anti-inflammatory moisturizer, with the anti-inflammatory moisturizer of the preparation of embodiment 1 the difference is that not containing vitamin E.
Comparative example 3:
A kind of anti-inflammatory moisturizer, with the anti-inflammatory moisturizer of the preparation of embodiment 1 the difference is that not containing rhizobia exocellular polysaccharide.
Performance detection:
Randomly selecting 20 20-40 years old faces has the volunteer of slight small pox, to moisturizing made from embodiment 1 and comparative example 1-3
Cream is tested, and is applied to face and is nursed, sHP88 moisture of skin tester and MEXAMETER MX16 skin-color
Plain instrument measures subject's moisture and pigment respectively, and the case where observe its facial small pox, service life is 1 month.After,
Variation before and after use is as shown in figures 1 and 2, it can be seen that using the volunteer of embodiment 1, skin of face significantly improves, flesh
Skin water content increases, and whitening skin, facial pigmentation depositional phenomenon makes moderate progress, and facial small pox and red and swollen situation obtain apparent
Improve;Using the volunteer of comparative example 1, face skin water content increases, and pigment deposition has significant change, but facial small pox without
It significantly improves;Using the volunteer of comparative example 2, face skin water content increases, and small pox is improved, but dermal melanin is heavy
Product phenomenon is without significantly improving;Using the volunteer of 3 facial mask of comparative example, facial pigmentation deposition and small pox are improved, still
Skin change of moisture content is not significant.
Detailed description of the invention
Fig. 1 is using the variation diagram to moisture content of skin before and after sample
Fig. 2 is using the variation diagram to skin pigment content before and after sample
By the above results as it can be seen that vitamin E is able to suppress melanin production, improve the obscure phenomenon of skin;Aloe polysaccharide energy
Enough significantly improve small pox flesh, have the function of inhibiting bacteria and diminishing inflammation, root nodule granulose can effective replenishing water and preserving moisture, make skin rich in bullet
Property.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (8)
1. the rhizobium fermentation composition used for cosmetic with anti-inflammatory moisture-keeping efficacy, which is characterized in that the composition is under
The effect of column parts by weight raw material composition:
4-8 parts of Arbutin, 5-10 parts of vitamin E, 10-20 parts of glycerol, 1-2 parts of butanediol, 1-3 parts of pulullan polysaccharide, hyalomitome
2-4 parts sour, 10-30 parts of rhizobia exocellular polysaccharide, 2-5 parts of aloe polysaccharide, 1-3 parts of ophiopogonpolysaccharide, 2-8 parts of allantoin, Radix Astragali
0.5-1.5 parts, 30-60 parts of water.
2. as described in claim 1, the rhizobium fermentation composition used for cosmetic with anti-inflammatory moisture-keeping efficacy, feature exists
In being made of raw material the effect of following parts by weight:
4 parts of Arbutin, 8 parts of vitamin E, 15 parts of glycerol, 1 part of butanediol, 3 parts of pulullan polysaccharide, 3 parts of hyaluronic acid, rhizobium
20 parts of exocellular polysaccharide, 5 parts of aloe polysaccharide, 3 parts of ophiopogonpolysaccharide, 5 parts of allantoin, 0.5 part of Radix Astragali, 50 parts of water.
3. such as according to claimed in claims 1-2, the rhizobium fermentation composition used for cosmetic with anti-inflammatory moisture-keeping efficacy, feature
It is, the sodium hyaluronate includes that three kinds of molecular weight, respectively 18-22 ten thousand, 10-18 ten thousand, 1-4 ten thousand, and proportion are 1:1:1;
And/or the pulullan polysaccharide molecular weight is 10-30 ten thousand.
4. the rhizobium fermentation composition used for cosmetic with anti-inflammatory moisture-keeping efficacy, feature exists as claimed in claims 1-2
In the number of rhizobium used in the rhizobia exocellular polysaccharide is CICC 10579.
5. the rhizobium fermentation composition used for cosmetic with anti-inflammatory moisture-keeping efficacy, feature exists as claimed in claims 1-2
In, the rhizobia exocellular polysaccharide the preparation method comprises the following steps: to maltose 20g/L, soy peptone 9 g/L, MgSO4•7H2O
0.2g/L, KH2PO4The rhizobium of access 3% in the sterilising medium of 0.41 g/L, NaCl 0.1g/L, 28 DEG C of 180rpm cultures
72h, obtains root nodule fermented liquid, adjusts PH to neutrality, filtration sterilization, alcohol precipitation, and freeze-drying obtains rhizobia exocellular polysaccharide.
6. the preparation side of the rhizobium fermentation composition used for cosmetic with anti-inflammatory moisture-keeping efficacy as described in claim 1-7
Method, which comprises the following steps:
(1) the good Radix Astragali of shape is selected, drying is cleaned, crosses No. six sieves, it is spare;
(2) Arbutin, pulullan polysaccharide, hyaluronic acid, allantoin, rhizobia exocellular polysaccharide, aloe polysaccharide are sequentially added mixed
It closes in liquid A, low-temperature heat stirs evenly to 40 DEG C, is down to room temperature, obtain mixed liquid B;
(3) glycerol, butanediol are added in mixed liquid B, is uniformly mixed, obtains mixed liquor C;
(4) vitamin E is added in mixed liquor C, stirs evenly to get anti-inflammatory moisture-keeping composition.
7. the preparation method of the rhizobium fermentation composition used for cosmetic with anti-inflammatory moisture-keeping efficacy as claimed in claim 6,
It is characterized in that, stirring rate is 1000rpm in step (2) and/or (3).
8. the method preparation as described in claim 1-7 has the rhizobium fermentation composition used for cosmetic of anti-inflammatory moisture-keeping efficacy,
The beneficial effect is that composition of the invention, can be effectively improved skin conditions, small pox is eliminated, improves red swelling of the skin situation,
The formation for preventing melanin keeps skin water profit exquisitely carved, and high resilience can be used for cosmetic skin.
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IT202000013933A1 (en) * | 2020-06-10 | 2021-12-10 | Azienda Consorziale Terme Di Comano | Bacterial strain with anti-inflammatory properties |
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JP2006008616A (en) * | 2004-06-28 | 2006-01-12 | Daiya Seiyaku Kk | Patch matrix and cosmetic composed thereof |
CN109010199A (en) * | 2018-10-19 | 2018-12-18 | 天津科技大学 | A kind of skin whitening, moisturizing composition and its preparation method and application |
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2019
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2006008616A (en) * | 2004-06-28 | 2006-01-12 | Daiya Seiyaku Kk | Patch matrix and cosmetic composed thereof |
CN109010199A (en) * | 2018-10-19 | 2018-12-18 | 天津科技大学 | A kind of skin whitening, moisturizing composition and its preparation method and application |
Non-Patent Citations (1)
Title |
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管凤贞等: "根瘤菌菌剂的研究与开发现状", 《生态学杂志》 * |
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IT202000013933A1 (en) * | 2020-06-10 | 2021-12-10 | Azienda Consorziale Terme Di Comano | Bacterial strain with anti-inflammatory properties |
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