TWM591990U - Porous carrier for culturing Antrodia cinnamomea - Google Patents

Porous carrier for culturing Antrodia cinnamomea Download PDF

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TWM591990U
TWM591990U TW108216793U TW108216793U TWM591990U TW M591990 U TWM591990 U TW M591990U TW 108216793 U TW108216793 U TW 108216793U TW 108216793 U TW108216793 U TW 108216793U TW M591990 U TWM591990 U TW M591990U
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antrodia camphorata
porous carrier
layer
cultivating
circumferential surface
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TW108216793U
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Chinese (zh)
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張瑞能
劉彥泓
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張瑞能
劉彥泓
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Priority to TW108216793U priority Critical patent/TWM591990U/en
Publication of TWM591990U publication Critical patent/TWM591990U/en

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Abstract

本創作提供一種用於培養牛樟芝的多孔載體,其具有一周面及複數凹入周面之微孔,多孔載體更具有一營養層及一披膜,營養層形成在周面及微孔的內周緣,披膜成型在營養層上,且披膜包覆營養層,藉此,本實施係在多孔載體形成營養層及披膜,以達到培育牛樟芝之目的。This creation provides a porous carrier for cultivating Antrodia camphorata, which has a circumferential surface and a plurality of micropores recessed into the circumferential surface. The porous carrier further has a nutrient layer and a coating, the nutrient layer is formed on the circumferential surface and the inner periphery of the micropores The mulch film is formed on the nutrition layer, and the mulch film covers the nutrition layer. Therefore, in this embodiment, the nutrition layer and the mulch film are formed on the porous carrier to achieve the purpose of cultivating Antrodia cinnamomea.

Description

用於培養牛樟芝的多孔載體Porous carrier for cultivating Antrodia camphorata

本創作係關於一種牛樟芝培育領域,尤指一種用於培養牛樟芝的多孔載體。This creation is about an Antrodia camphorata cultivation field, especially a porous carrier used to cultivate Antrodia camphorata.

按,牛樟芝於自然環境中只生長在台灣特有種的牛樟樹上,其中,目前牛樟樹歸類為瀕危的保育類植物,數量稀少,以致於野生牛樟芝不易取得,因此人工培育牛樟芝不論在學術界或是產業界均為重要的研究指標。According to the press, Antrodia camphorata grows only in the native Antrodia camphorata trees that are endemic to Taiwan. Among them, the Antrodia camphorata is currently classified as an endangered conservation plant, and the number is so rare that wild Antrodia camphorata is not easy to obtain. Or the industry is an important research indicator.

目前國內對於牛樟芝的人工培育法主要可分為:段木栽培法、液體發酵法與固體培養法。就以段木栽培法及液體發酵法來說,段木栽培法是利用牛樟芝的牛樟原木或是其他近似物種的段木作為培養基,進而栽培牛樟芝的子實體,其中,此種方法所培育出的牛樟芝子實體能具有與野生牛樟芝子實體相同或近似的活性成份與功效,然而,前述段木栽培法所需的牛樟原木是台灣一級保育木不能砍伐,因而牛樟原木不易取得,且牛樟段木培養需百年以上的培植期,因此大幅提高牛樟芝的培養成本,故此種方法並非一般學術界或產業界所能負擔。At present, the artificial cultivation methods for Antrodia camphorata in China can be divided into: section wood cultivation method, liquid fermentation method and solid cultivation method. In terms of segment wood cultivation method and liquid fermentation method, the segment wood cultivation method is to use Antrodia camphorata logs or other similar species of segment wood as the culture medium, and then cultivate the fruit body of Antrodia camphorata, which is cultivated by this method The Antrodia camphorata fruiting bodies can have the same or similar active ingredients and effects as the wild Antrodia camphorata fruiting bodies. Camphor tree cultivation requires a cultivation period of more than 100 years, so the cultivation cost of Antrodia camphorata is greatly increased, so this method is not affordable by the general academic or industrial circles.

另外,液體發酵法是利用液態培養基進行牛樟芝菌絲體的發酵培養,其中,此種方法培養時間短且成本相當低廉,產業利用性高;但是,前述液體發酵法所培養牛樟芝菌絲體的活性成份的含量較低,無法得到野生牛樟芝特有的三萜類成分。In addition, the liquid fermentation method is the fermentation culture of Antrodia cinnamomea mycelium using a liquid medium. Among them, this method has a short cultivation time, a relatively low cost, and high industrial applicability; however, the activity of the Antrodia cinnamomea mycelium cultured by the aforementioned liquid fermentation method The content of the ingredients is low, and the triterpenoids unique to wild Antrodia camphorata cannot be obtained.

為解決上述課題,本創作實施例提供一種用於培養牛樟芝的多孔載體,其利用輕易取得的多孔載體取代傳統段木培養所需的牛樟原木,進而降低培養成本,且多孔載體形成營養層及披膜,能促進牛樟菌生長,使本實施所培養的牛樟芝子實體含有三萜類的活性成份與功效。In order to solve the above-mentioned problems, this creative embodiment provides a porous carrier for cultivating Antrodia camphorata, which uses an easily obtained porous carrier to replace the Antrodia camphora logs required for traditional segmental wood cultivation, thereby reducing the cultivation cost, and the porous carrier forms a nutrient layer and The mulching film can promote the growth of Antrodia camphorata, and the fruiting body of Antrodia camphorata cultivated in this implementation contains active ingredients and effects of triterpenes.

為達到上述目的,本創作實施例提供一種用於培養牛樟芝的多孔載體,其具有一周面及複數凹入周面之微孔,多孔載體更具有一營養層及一披膜,營養層形成在周面及微孔的內周緣,披膜成型在營養層上,且披膜包覆營養層。In order to achieve the above purpose, the present embodiment provides a porous carrier for cultivating Antrodia camphorata, which has a circumferential surface and a plurality of micropores recessed into the circumferential surface. The porous carrier further has a nutrient layer and a coating, the nutrient layer is formed on the periphery On the inner periphery of the surface and the micropores, the coating is formed on the nutrition layer, and the coating covers the nutrition layer.

因此,本創作係在多孔載體形成營養層及披膜,將牛樟菌接種在多孔載體的周面及微孔,後續再將多孔載體置於密封環境進行恆溫、恆濕培養,以模擬牛樟菌的生長環境,且營養層與披膜之成分能促進牛樟菌生長,據以本實施所培養的牛樟芝子實體含有三萜類的活性成份與功效,達到有效培育牛樟芝之目的。Therefore, this creative system forms a nutrient layer and a film on the porous carrier, inoculates the camphor bacteria on the peripheral surface and micropores of the porous carrier, and then places the porous carrier in a sealed environment for constant temperature and constant humidity culture to simulate the camphor The growth environment of the bacteria, and the composition of the nutrition layer and the mulch film can promote the growth of Antrodia camphorata. According to this implementation, the Antrodia camphorata fruiting body contains triterpenoid active ingredients and effects, so as to effectively cultivate the Antrodia camphorata.

為便於說明本創作於上述新型內容一欄中所表示的中心思想,茲以具體實施例表達。In order to facilitate the explanation of the central idea expressed in the column of the above-mentioned new content, the specific embodiment is hereby expressed.

請參閱圖1至圖5所示,本創作實施例提供一種用於培養牛樟芝的多孔載體10,其中,多孔載體10為非塑膠材質製成之立方型體,於一較佳實施例中,多孔載體10包含磚頭、石粉壓縮塊、陶瓦、陶土、瓷土、防火磚之其中一種,如圖2所示本創作實施例以防火磚進行說明,多孔載體10具有一周面11及凹入周面11之複數微孔12,微孔12分布於多孔載體10內部且相連通,且多孔載體10更形成有一營養層13及一披膜14,營養層13形成在多孔載體10之周面11及微孔12的內周緣,披膜14成型在營養層13上。Please refer to FIG. 1 to FIG. 5, this creative embodiment provides a porous carrier 10 for cultivating Antrodia camphorata, wherein the porous carrier 10 is a cubic body made of non-plastic material. In a preferred embodiment, porous The carrier 10 includes one of bricks, compressed stone powder blocks, terracotta, clay, porcelain clay, and fireproof bricks. As shown in FIG. 2, this creative embodiment is illustrated by fireproof bricks. The porous carrier 10 has a circumferential surface 11 and a concave circumferential surface 11 A plurality of micropores 12, which are distributed inside the porous carrier 10 and communicate with each other, and the porous carrier 10 is further formed with a nutrient layer 13 and a coating 14, the nutrient layer 13 is formed on the peripheral surface 11 of the porous carrier 10 and the micropores At the inner periphery of 12, a film 14 is formed on the nutrition layer 13.

更進一步說明,請配合圖1至圖5所示,本創作牛樟芝培育方法係運用前述多孔載體10進行培育牛樟芝,牛樟芝培育方法包含下列步驟:For further explanation, please refer to FIG. 1 to FIG. 5, the cultivation method of Antrodia camphorata is to use the aforementioned porous carrier 10 to cultivate Antrodia camphorata. The cultivation method of Antrodia camphorata includes the following steps:

步驟一S1:依比例混合一營養液,營養液的組成包含牛樟樹萃取物、碳源、氮源,於一較佳實施例中,牛樟樹萃取物為牛樟枝條及牛樟葉經由均質及萃取而得,提供促進牛樟菌生長之效用,碳源為葡萄糖及蔗糖,氮源為酵母菌萃取物,後續將營養液設置在多孔載體10,舉例來說,本創作多孔載體10係浸泡在營養液中,使營養液能滲入於多孔載體10之微孔12,再將濕潤的多孔載體10進行35度至45度烘乾,以防止營養液的成分變質,致使營養液凝固成營養層13而成型在多孔載體10之周面11及微孔12的內周緣。Step 1 S1: Mixing a nutrient solution in proportion, the composition of the nutrient solution includes Antrodia camphorata extract, carbon source, and nitrogen source. In a preferred embodiment, the Antrodia camphorata extract is Antrodia camphorata branches and Antrodia camphorata leaves through homogenization and extraction In order to provide the effect of promoting the growth of Antrodia camphorata, the carbon source is glucose and sucrose, the nitrogen source is yeast extract, and then the nutrient solution is set on the porous carrier 10, for example, the porous carrier 10 of this creation is soaked in nutrition In the solution, the nutrient solution can penetrate into the pores 12 of the porous carrier 10, and then the wet porous carrier 10 is dried at 35 degrees to 45 degrees to prevent the components of the nutrient solution from deteriorating, causing the nutrient solution to solidify into the nutrient layer 13 and Molded on the peripheral surface 11 of the porous carrier 10 and the inner peripheral edge of the micropore 12.

步驟二S2:接著,多孔載體10進行膜化處理,在營養層13上形成有披膜14,其中,披膜14由食品添加劑、牛樟芝萃取物及醣類混合組成,於一較佳實施例中,食品添加劑為聚乙二醇(Polyethylene glycol,簡稱PEG),牛樟芝萃取物為牛樟芝粉末與牛樟菌絲體經由均質及萃取而得,醣類為蔗糖或葡萄糖,其中,披膜14實施過程係先將食品添加劑加熱成液態,再加入牛樟芝萃取物及醣類混合成塗料,後續將塗料塗覆在多孔載體10之營養層13上,使塗料能在多孔載體10之周面11及微孔12的營養層13冷卻形成披膜14,以致於披膜14包覆營養層13,作為阻隔營養層13與外界接觸之效用,如圖3及圖3A所示。Step 2 S2: Next, the porous carrier 10 is subjected to a membrane treatment, and a coating film 14 is formed on the nutrition layer 13, wherein the coating film 14 is composed of a mixture of food additives, Antrodia cinnamomea extract and sugar, in a preferred embodiment , The food additive is Polyethylene glycol (Polyethylene glycol, referred to as PEG), Antrodia camphorata extract is obtained by homogenization and extraction of Antrodia camphorata powder and Antrodia camphorata mycelium, the sugar is sucrose or glucose, and the process of coating 14 is The food additive is first heated to a liquid state, and then the Antrodia camphorata extract and sugar are mixed to form a coating, and then the coating is coated on the nutrition layer 13 of the porous carrier 10, so that the coating can be on the peripheral surface 11 and the micropores 12 of the porous carrier 10 The nutrition layer 13 is cooled to form a coating film 14 so that the coating film 14 covers the nutrition layer 13 as a function of blocking the nutrition layer 13 from contacting the outside world, as shown in FIGS. 3 and 3A.

步驟三S3:多孔載體10進行滅菌處理,於一較佳實施例中,多孔載體10係經由照射伽瑪射線,對多孔載體10上的披膜14及營養層13進行輻射滅菌的程序。Step 3 S3: The porous carrier 10 is sterilized. In a preferred embodiment, the porous carrier 10 is a procedure of sterilizing the coating 14 and the nutrition layer 13 on the porous carrier 10 by irradiating gamma rays.

步驟四S4:最後,將牛樟菌接種在多孔載體10的周面11及微孔12的披膜14上,再將多孔載體10置於密封環境進行恆溫、恆濕培養,俾使披膜14能被牛樟菌分解,且營養層13能提供牛樟菌生長成牛樟芝子實體30,於一較佳實施例中,請配合圖4所示,多孔載體10係密封在一生長箱20中,生長箱20包含一容器21及一外蓋22,容器21具有一容置空間211,外蓋22蓋設於容器21的開口而使容置空間211呈密封環境;此外,生長箱20在容器21內設有一固定架23,固定架23能提供多孔載體10架高固定,且生長箱20在容器21中更填充有無菌水24而不接觸多孔載體10,使生長箱20內能密閉保持濕度,在培養過程中避免光照,生長箱20內部溫度維持在25度至28度、濕度維持在60%至80%,以模擬牛樟菌的生長環境,其中,多孔載體10所形成營養層13與披膜14之成分能促進牛樟菌生長,且微孔12的內周緣營養層13含量大於周面11的營養層13含量,因此牛樟菌能由多孔載體10的微孔12沿著周圍的周面11生長。Step 4 S4: Finally, inoculate antrodia camphorata on the circumferential surface 11 of the porous carrier 10 and the coating film 14 of the micropores 12, and then place the porous carrier 10 in a sealed environment for constant temperature and constant humidity culture to make the coating film 14 It can be decomposed by Antrodia camphorata, and the nutrition layer 13 can provide the growth of Antrodia camphorata into Antrodia camphorata fruiting body 30. In a preferred embodiment, as shown in FIG. 4, the porous carrier 10 is sealed in a growth box 20. The growth box 20 includes a container 21 and an outer cover 22. The container 21 has an accommodating space 211. The outer cover 22 covers the opening of the container 21 to make the accommodating space 211 a sealed environment. In addition, the growth box 20 is in the container 21 A fixing frame 23 is provided inside, the fixing frame 23 can provide a fixed height of the porous carrier 10, and the growth box 20 is filled with sterile water 24 in the container 21 without contacting the porous carrier 10, so that the growth box 20 can be sealed and maintain humidity, To avoid light during the cultivation process, the internal temperature of the growth box 20 is maintained at 25 degrees to 28 degrees, and the humidity is maintained at 60% to 80%, to simulate the growth environment of Antrodia camphorata, in which the nutrient layer 13 and the coating formed by the porous carrier 10 The composition of the membrane 14 can promote the growth of Antrodia camphorata, and the content of the nutrition layer 13 on the inner periphery of the micropores 12 is greater than the content of the nutrition layer 13 on the peripheral surface 11, so the Antrodia camphorata can be guided by the micropores 12 of the porous carrier 10 along the surrounding Face 11 grows.

再者,本創作生長箱20係為透明材質,使用者能透過生長箱20直接觀察牛樟菌的生長狀況,並定期利用針筒對多孔載體10適量地補充營養液,提供牛樟菌生長所需之養分,值得一提的是,本創作經3個月觀察發現,多孔載體10的周面11及微孔12能明顯生長牛樟菌絲,再經8個月觀察發現,多孔載體10的周面11確實長出牛樟芝子實體30,如圖5所示。In addition, the growth box 20 of the present creation is made of transparent material. The user can directly observe the growth status of Antrodia camphorata through the growth box 20, and regularly supplement the porous carrier 10 with an appropriate amount of nutrient solution using a syringe to provide an growth site for antrodia camphorata For the nutrients needed, it is worth mentioning that after 3 months of observation in this work, the circumferential surface 11 and micropores 12 of the porous carrier 10 can obviously grow Antrodia camphorata mycelium, and after 8 months of observation, the porous carrier 10 Peripheral surface 11 does indeed grow Antrodia camphorata fruiting body 30, as shown in FIG. 5.

因此,本創作牛樟芝培育方法係在多孔載體10形成營養層13及披膜14,將牛樟菌接種在多孔載體10的周面11及微孔12,後續再將多孔載體10置於密封環境進行恆溫、恆濕培養,以模擬牛樟菌的生長環境,且營養層13與披膜14之成分能促進牛樟菌生長,據以本實施所培養的牛樟芝子實體30含有三萜類的活性成份與功效,達到有效培育牛樟芝之目的。Therefore, the cultivation method of Antrodia camphorata is to form a nutrient layer 13 and a coating 14 on a porous carrier 10, inoculate Antrodia camphorata on the peripheral surface 11 and micropores 12 of the porous carrier 10, and then place the porous carrier 10 in a sealed environment Constant temperature and constant humidity culture to simulate the growth environment of Antrodia camphorata, and the composition of nutrient layer 13 and film 14 can promote the growth of Antrodia camphorata. According to this implementation, the fruit body 30 of Antrodia camphorata contains triterpenoid active ingredients And effect, to achieve the purpose of effective cultivation of Antrodia camphorata.

再者,本實施利用輕易取得的多孔載體10取代傳統段木培養所需的牛樟原木,進而降低培養成本,使本創作牛樟芝培育方法能提供一般學術界及產業界進行研究使用。In addition, this implementation uses an easily obtained porous carrier 10 to replace the log of Antrodia camphorata needed for traditional segmental wood cultivation, thereby reducing the cost of cultivation, so that the creative Antrodia camphorata cultivation method can be provided for general academic and industrial research and use.

藉此本創作實施例揭露如上,然其並非用以限定本創作,在本創作所屬技術領域中任何具有通常知識者,在不脫離本創作之精神和範圍內,當可作各種之更動與潤飾,因此本創作之保護範圍當視後附之申請專利範圍所界定者為準。This embodiment of the creation is disclosed as above, but it is not used to limit the creation. Anyone with ordinary knowledge in the technical field to which the creation belongs can be used for various changes and retouching without departing from the spirit and scope of the creation. Therefore, the scope of protection of this creation shall be deemed as defined by the scope of the attached patent application.

10:多孔載體 22:外蓋 11:周面 23:固定架 12:微孔 24:無菌水 13:營養層 30:牛樟芝子實體 14:披膜 S1:步驟一 20:生長箱 S2:步驟二 21:容器 S3:步驟三 211:容置空間 S4:步驟四10: porous carrier 22: outer cover 11: Weekly 23: fixed frame 12: Microwell 24: Sterile water 13: Nutrition layer 30: Antrodia cinnamomea fruiting body 14: Mantle S1: Step one 20: Growth box S2: Step two 21: Container S3: Step three 211: accommodating space S4: Step 4

圖1係為本創作實施例之步驟流程圖 圖2係為本創作實施例多孔載體之立體示意圖。 圖3係為本創作實施例之剖面示意圖。 圖3A係圖3之A部分的局部放大圖。 圖4係為本創作實施例之實施示意圖。 圖5係為本創作實施例牛樟芝子實體之生長狀態示意圖。 Figure 1 is a flow chart of steps in this creative embodiment FIG. 2 is a three-dimensional schematic diagram of a porous carrier in this creative example. FIG. 3 is a schematic cross-sectional view of an embodiment of the creation. FIG. 3A is a partially enlarged view of part A of FIG. 3. Fig. 4 is a schematic diagram of an implementation of the creative embodiment. FIG. 5 is a schematic diagram of the growth state of Antrodia camphorata fruiting bodies according to the embodiment of the present invention.

10:多孔載體 10: porous carrier

11:周面 11: Weekly

12:微孔 12: Microwell

13:營養層 13: Nutrition layer

14:披膜 14: Mantle

Claims (5)

一種用於培養牛樟芝的多孔載體,其具有一周面及複數凹入該周面之微孔,該多孔載體更具有一營養層及一披膜,該營養層形成在該周面及該等微孔的內周緣,該披膜成型在該營養層上,且該披膜包覆該營養層。A porous carrier for cultivating Antrodia camphorata, which has a circumferential surface and a plurality of micropores recessed into the circumferential surface, the porous carrier further has a nutrient layer and a coating, the nutrient layer is formed on the circumferential surface and the micropores On the inner periphery of the film, the coating film is formed on the nutrition layer, and the coating film covers the nutrition layer. 如請求項1所述之用於培養牛樟芝的多孔載體,其中,該多孔載體為非塑膠材質製成之立方型體。The porous carrier for cultivating Antrodia camphorata according to claim 1, wherein the porous carrier is a cubic body made of non-plastic material. 如請求項2所述之用於培養牛樟芝的多孔載體,其中,該多孔載體包含磚頭、石粉壓縮塊、陶瓦、陶土、瓷土、防火磚之其中一種。The porous carrier for cultivating Antrodia camphorata according to claim 2, wherein the porous carrier comprises one of bricks, compressed stone powder blocks, ceramic tiles, clay, porcelain clay, and fireproof bricks. 如請求項1所述之用於培養牛樟芝的多孔載體,其中,該營養層包含牛樟樹萃取物、碳源、氮源,牛樟樹萃取物為牛樟枝條及牛樟葉經由均質及萃取而得,碳源為葡萄糖及蔗糖,氮源為酵母菌萃取物。The porous carrier for cultivating Antrodia camphorata according to claim 1, wherein the nutrient layer contains antrodia camphorata extract, carbon source, and nitrogen source, and the antrodia camphorata extract is obtained by homogenizing and extracting antrodia camphorata branches and antrodia camphorata leaves, The carbon source is glucose and sucrose, and the nitrogen source is yeast extract. 如請求項1所述之用於培養牛樟芝的多孔載體,其中,該披膜由食品添加劑、牛樟芝萃取物及醣類混合組成,食品添加劑為聚乙二醇,牛樟芝萃取物為牛樟芝粉末與牛樟菌絲體經由均質及萃取而得,醣類為蔗糖或葡萄糖。The porous carrier for cultivating Antrodia camphorata as described in claim 1, wherein the coating is composed of a mixture of food additives, Antrodia camphorata extract and sugar, the food additive is polyethylene glycol, and the Antrodia camphorata extract is Antrodia camphorata powder and Antrodia camphorata The mycelium is obtained by homogenization and extraction, and the sugar is sucrose or glucose.
TW108216793U 2019-12-17 2019-12-17 Porous carrier for culturing Antrodia cinnamomea TWM591990U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021143254A1 (en) * 2020-01-15 2021-07-22 张瑞能 Antrodia cinnamomea cultivation method and porous carrier for cultivating antrodia cinnamomea
TWI813997B (en) * 2021-05-18 2023-09-01 秦文隆 Carrier for Cultivating Antrodia Antrodia and Its Manufacturing Method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021143254A1 (en) * 2020-01-15 2021-07-22 张瑞能 Antrodia cinnamomea cultivation method and porous carrier for cultivating antrodia cinnamomea
TWI813997B (en) * 2021-05-18 2023-09-01 秦文隆 Carrier for Cultivating Antrodia Antrodia and Its Manufacturing Method

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