CN105543101A - Antrodia camphorate mother strain preparation method - Google Patents
Antrodia camphorate mother strain preparation method Download PDFInfo
- Publication number
- CN105543101A CN105543101A CN201510653051.9A CN201510653051A CN105543101A CN 105543101 A CN105543101 A CN 105543101A CN 201510653051 A CN201510653051 A CN 201510653051A CN 105543101 A CN105543101 A CN 105543101A
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- water
- camphor tree
- antrodia camphorata
- preparation
- mother
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- 241001486992 Taiwanofungus camphoratus Species 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 241000723346 Cinnamomum camphora Species 0.000 claims abstract description 29
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 21
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 7
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 7
- 235000012015 potatoes Nutrition 0.000 claims abstract description 7
- 229920001817 Agar Polymers 0.000 claims abstract description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 4
- 239000008272 agar Substances 0.000 claims abstract description 4
- 239000008103 glucose Substances 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- 210000002686 mushroom body Anatomy 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 235000013372 meat Nutrition 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 12
- 238000012258 culturing Methods 0.000 abstract description 2
- 239000002023 wood Substances 0.000 abstract 3
- 238000005507 spraying Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 241000723347 Cinnamomum Species 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 241000207961 Sesamum Species 0.000 description 3
- 235000003434 Sesamum indicum Nutrition 0.000 description 3
- 240000007313 Tilia cordata Species 0.000 description 3
- 241000219071 Malvaceae Species 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 241000609240 Ambelania acida Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 241000222336 Ganoderma Species 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000004611 cancer cell death Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000010979 ruby Substances 0.000 description 1
- 229910001750 ruby Inorganic materials 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- -1 triterpene compound Chemical class 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
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- Mushroom Cultivation (AREA)
Abstract
The invention relates to an Antrodia camphorate mother strain preparation method. The method comprises the following steps: 1, preparing a medium: taking a camphor tree wood block, disposing a basin for containing water below the wood blocks, spraying the camphor tree wood block with water once every 2-5d, collecting camphor tree water in the basin, taking 500ml of the camphor tree water, and mixing the 500ml of the camphor tree water with 1500ml of water, 35-39g of glucose, 180-210g of peeled potatoes and 19-23g of agar to prepare the medium; 2, separating an Antrodia camphorate component: taking 0.2-0.7cm mushroom dices, and inoculating the mushroom dices to a culture dish containing the medium; and 3, culturing an Antrodia camphorate mother strain. The Antrodia camphorate mother strain preparation method has the advantages of low cost and high Antrodia camphorate quality.
Description
Technical field
The present invention relates to a kind of spawn culture field, particularly a kind of Antrodia camphorata mother plants preparation method.
Background technology
Antrodia camphorata is called for short " camphor tree sesame ", and formal name used at school is Antrodiacamphorata, mushroom, camphor tree mushroom, blood glossy ganoderma etc. in popular name Cinnamomum kanahirai hay mushroom, red camphor tree sesame, camphor tree, and because of the special mystery of its curative effect, to control people countless, at the title even having " negative and positive are to mushroom " among the people.Composition triterpenes in Antrodia camphorata is proved to be can promote cancer cell death, suppress liver cancer cell increment, repair liver, promote liver function, anti-inflammation, reducing blood-fat, hypotensive, prevent the effect such as apoplexy and immunity moderation, therefore have the good reputation of " king of medicine sesame ", " ruby in the forest of Taiwan ".
What the Antrodia camphorata cultural method of prior art was relatively more conventional has solid medium cultivation, liquid fermentation method, cultivation basswood method.Solid medium cultivation, take one piece about 1 centimeter square Antrodia camphorata mycelium or sporophore, and be attached at the surface of solid medium, this Antrodia camphorata is enable to be attached at the surface growth of this solid medium, although the solid medium cultivation of prior art can obtain mycelium or the sporophore of Antrodia camphorata, and more not easily produce miscellaneous bacteria, but, triterpene compound but only containing very low amounts in the mycelium that this method obtains or sporophore, therefore the Antrodia camphorata mycelium that obtains of the solid medium cultivation of prior art or sporophore, cannot obtain industrialization and use.
Liquid fermentation method, that the mycelium of Antrodia camphorata is inoculated in liquid nutrient solution, although this liquid nutrient solution can improve physiologically active substance contained in Antrodia camphorata, but need in culturing process to add if the solid substrate such as bagasse or five cereals is as the mycelial upholder of Antrodia camphorata, therefore, liquid fermentation method must with the bioreactor culture of large volume, and its preparation cost is high, and the situation of living contaminants also easily occurs liquid fermentation method.
Cultivation basswood method, be seeded in by Antrodia camphorata on Cinnamomum kanahirai hay linden, the kind of the Antrodia camphorata composition that it obtains and the physiologically active substance contained by wild Antrodia camphorata is close.For first two method, the Antrodia camphorata quality that this kind of method cultivates is higher.But because Cinnamomum kanahirai hay linden obtains not easily, cultivate and need a lot of Cinnamomum kanahirai hay linden, cost is high.
Summary of the invention
The object of the present invention is to provide a kind of preparation method, make the Antrodia camphorata active component content that cultivates high and the cost expended is low.
This Antrodia camphorata mother of the present invention plants preparation method, comprises the following steps:
(1) substratum preparation steps, described substratum preparation steps comprises camphor tree water preparation steps, and described camphor tree water preparation steps is, get camphor tree wooden unit, be placed with a basin be filled with water below wooden unit, within 2 ~ 5 days, spray a water for camphor tree wooden unit, collect the camphor tree water in basin; Get described camphor tree water 500ml, water 1500ml to mix, add peeled potatoes 180 ~ 210g and boil; By the water filtration of boiled potatoes, get filtrate 1000ml and add agar 19 ~ 23g, add glucose 35 ~ 39g after boiling, be mixed with mixing solutions, described mixing solutions is boiled, after making it dissolve completely, make described mixing solutions be frozen into substratum;
(2) Component seperation step, comprises and being cut by Antrodia camphorata mushroom body, gets the inner meat of mushroom body, is cut into 0.2 ~ 0.7cm mushroom block, is inoculated in the culture dish filling described substratum;
(3) Mother culture step, cultivate at culture dish being placed in 25 DEG C, by growing up, mycelia is fast cut, and transplants and puts in new culture dish, through transplanting repeatedly, turns out Antrodia camphorata mother and plants.
Preferably, in described substratum preparation steps, the substratum prepared was 115 ~ 125 DEG C of sterilizings 30 ~ 50 minutes.
Preferably, in described Component seperation step, after mushroom block is cut, with clean gauze or sack, mushroom block is wrapped, put into Bechtop, irradiate 25 ~ 35 minutes under ultraviolet light.
Preferably, in described Component seperation step, the clean gauze wiping speckling with 75% ethanol of mushroom surface, then cut mushroom block.
In excellent described Antrodia camphorata Mother culture step, culture dish surface multilayer gauze or jaconet cover, lucifuge.
By getting camphor tree wooden unit in substratum configuration step of the present invention, regular spray water, the camphor tree water collected in basin carries out substratum preparation, improves Antrodia camphorata active constituent content, improves the Antrodia camphorata speed of growth, at utmost saves cost simultaneously; In separating step, uviolizing and 75% alcohol disinfecting, can reduce infection rate; In Mother culture step, arrange suitable culture temperature, improve the Antrodia camphorata mycelium of manufacture in prior art or the of low quality of sporophore, Antrodia camphorata quality is higher, improves the speed of growth.
Accompanying drawing explanation
Fig. 1 is the step sketch that a kind of Antrodia camphorata mother of the present invention plants preparation method;
Drawing reference numeral illustrates: S1, substratum preparation steps; S2, Component seperation step; S3, Mother culture step.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail.
Embodiment one:
(1) substratum preparation steps S1, described substratum preparation steps comprises camphor tree water preparation steps, and described camphor tree water preparation steps is, get camphor tree wooden unit, be placed with a basin be filled with water below wooden unit, within 2 ~ 5 days, spray a water for camphor tree wooden unit, collect the camphor tree water in basin; Get described camphor tree water 500ml, water 1500ml to mix, add peeled potatoes 200g and boil; By the water filtration of boiled potatoes, get filtrate 1000ml and add agar 21g, add glucose 37g after boiling, be mixed with mixing solutions, described mixing solutions is boiled, after making it dissolve completely, make described mixing solutions be frozen into substratum; The substratum prepared was 121 DEG C of sterilizings 30 ~ 50 minutes.
(2) Component seperation step S2, comprise first by mushroom surface with after the clean gauze wiping speckling with 75% ethanol, then cut mushroom block.Antrodia camphorata mushroom body cuts, and gets the inner meat of mushroom body, is cut into 0.2 ~ 0.7cm mushroom block, after mushroom block is cut, with clean gauze or sack, mushroom block is wrapped, put into Bechtop, irradiate 25 ~ 35 minutes under ultraviolet light, be inoculated in the culture dish filling described substratum.
(3) Mother culture step S3, cultivate at culture dish being placed in 25 DEG C, when mycelia grows to 2/3rds culture dish, by growing up, mycelia is fast cut, and transplants and puts in new culture dish, through the transplanting of 2 ~ 3 times, turns out Antrodia camphorata mother and plants.Culture dish surface multilayer gauze or jaconet cover, lucifuge.
Choose the mycelia that 10 parts of embodiment of the present invention one are cultivated, 10 parts of Mother culture temperature are the mycelia of 23.5 DEG C, and 10 parts of Mother culture temperature are the mycelia of 26.5 DEG C, and contrast, comparing result is as follows:
Mycelia is brick-red, illustrates that mycelial growth situation is good, and shallow orange illustrates that upgrowth situation is general.Mycelium germination circle radius is larger, illustrates that the speed of growth of mycelia is faster.
When Mother culture temperature is lower than 23.5 DEG C or higher than 26.5 DEG C, mycelia color is grey black, shows the non-normal growth of mycelia.
Therefore embodiments of the invention 25 DEG C are optimum culture temperature, and Antrodia camphorata mycelial growth rate is fast, and mycelia is superior in quality.
In sum, these are only preferred embodiment of the present invention, be not intended to limit protection scope of the present invention.All any amendments done within the spirit and principles in the present invention, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1. Antrodia camphorata mother plants a preparation method, it is characterized in that, comprises the following steps:
(1) substratum preparation steps, described substratum preparation steps comprises camphor tree water preparation steps, and described camphor tree water preparation steps is, get camphor tree wooden unit, be placed with a basin be filled with water below wooden unit, within 2 ~ 5 days, spray a water for camphor tree wooden unit, collect the camphor tree water in basin; Get described camphor tree water 500ml, water 1500ml to mix, add peeled potatoes 180 ~ 210g and boil; By the water filtration of boiled potatoes, get filtrate 1000ml and add agar 19 ~ 23g, add glucose 35 ~ 39g after boiling, be mixed with mixing solutions, described mixing solutions is boiled, after making it dissolve completely, make described mixing solutions be frozen into substratum;
(2) Component seperation step, comprises and being cut by Antrodia camphorata mushroom body, gets the inner meat of mushroom body, is cut into 0.2 ~ 0.7cm mushroom block, is inoculated in the culture dish filling described substratum;
(3) Mother culture step, cultivate at culture dish being placed in 25 DEG C, by growing up, mycelia is fast cut, and transplants and puts in new culture dish.
2. a kind of Antrodia camphorata mother according to claim 1 plants preparation method, and it is characterized in that, in described substratum preparation steps, the substratum prepared was 115 ~ 125 DEG C of sterilizings 30 ~ 50 minutes.
3. a kind of Antrodia camphorata mother according to claim 1 plants preparation method, it is characterized in that, in described Component seperation step, after being cut by mushroom block, is wrapped by mushroom block, put into Bechtop, irradiate 25 ~ 35 minutes under ultraviolet light with clean gauze or sack.
4. a kind of Antrodia camphorata mother according to claim 1 plants preparation method, it is characterized in that, in described Component seperation step, and the clean gauze wiping speckling with 75% ethanol of mushroom surface, then cut mushroom block.
5. a kind of Antrodia camphorata mother according to claim 1 plants preparation method, it is characterized in that, in described Mother culture step, culture dish surface multilayer gauze or jaconet cover lucifuge.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510653051.9A CN105543101B (en) | 2015-10-11 | 2015-10-11 | A kind of Antrodia camphorata parent species preparation method |
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| CN201510653051.9A CN105543101B (en) | 2015-10-11 | 2015-10-11 | A kind of Antrodia camphorata parent species preparation method |
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| CN105543101A true CN105543101A (en) | 2016-05-04 |
| CN105543101B CN105543101B (en) | 2018-09-04 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235698A (en) * | 2019-07-23 | 2019-09-17 | 漳州帝宝牛樟芝生物科技股份有限公司 | A kind of cultural method of Antrodia camphorata |
| CN113040005A (en) * | 2021-01-15 | 2021-06-29 | 张颖 | Antrodia camphorata YZZ-09-XZ-01 mother strain culture medium and application thereof |
| CN113151012A (en) * | 2021-05-11 | 2021-07-23 | 河北大河生物科技有限公司 | High-density fermentation method of antrodia camphorata |
| CN113174338A (en) * | 2021-05-28 | 2021-07-27 | 广西壮族自治区农业科学院 | Black fungus tissue isolation culture medium and application thereof |
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| US20090145023A1 (en) * | 2007-11-30 | 2009-06-11 | His-Hisung Huang | Artificial cultivating method of fruiting body of antrodia camphorata |
| CN103733881A (en) * | 2013-12-23 | 2014-04-23 | 广东省微生物研究所 | Production method of antrodia camphorata stock seeds |
| CN104718969A (en) * | 2013-12-18 | 2015-06-24 | 于汇 | Production technology and using method for edible mushroom branch mother seeds |
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2015
- 2015-10-11 CN CN201510653051.9A patent/CN105543101B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090145023A1 (en) * | 2007-11-30 | 2009-06-11 | His-Hisung Huang | Artificial cultivating method of fruiting body of antrodia camphorata |
| CN104718969A (en) * | 2013-12-18 | 2015-06-24 | 于汇 | Production technology and using method for edible mushroom branch mother seeds |
| CN103733881A (en) * | 2013-12-23 | 2014-04-23 | 广东省微生物研究所 | Production method of antrodia camphorata stock seeds |
Non-Patent Citations (2)
| Title |
|---|
| MADAMANCHI GEETHANGILI ET AL.: "Review of Pharmacological Effects of Antrodia camphorata and Its Bioactive Compounds", 《EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE》 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110235698A (en) * | 2019-07-23 | 2019-09-17 | 漳州帝宝牛樟芝生物科技股份有限公司 | A kind of cultural method of Antrodia camphorata |
| CN113040005A (en) * | 2021-01-15 | 2021-06-29 | 张颖 | Antrodia camphorata YZZ-09-XZ-01 mother strain culture medium and application thereof |
| CN113151012A (en) * | 2021-05-11 | 2021-07-23 | 河北大河生物科技有限公司 | High-density fermentation method of antrodia camphorata |
| CN113174338A (en) * | 2021-05-28 | 2021-07-27 | 广西壮族自治区农业科学院 | Black fungus tissue isolation culture medium and application thereof |
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| Publication number | Publication date |
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| CN105543101B (en) | 2018-09-04 |
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Address after: 363800 Guangu Village, Shajian Town, Huaan County, Zhangzhou City, Fujian Province Patentee after: Fujian Zhongyan Mushroom Industry Co.,Ltd. Address before: 363800 Guangu Village, Shajian Town, Huaan County, Zhangzhou City, Fujian Province Patentee before: FUJIAN ZHONGYAN MUSHROOM Co.,Ltd. |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method for preparing the mother seed of Ganoderma lucidum Effective date of registration: 20231221 Granted publication date: 20180904 Pledgee: Bank of China Limited by Share Ltd. Zhangzhou Huaan branch Pledgor: Fujian Zhongyan Mushroom Industry Co.,Ltd. Registration number: Y2023980073044 |
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