TWI799778B - 均一含有具有左右非對稱之糖鏈之抗體之抗體群、及其製造方法 - Google Patents

均一含有具有左右非對稱之糖鏈之抗體之抗體群、及其製造方法 Download PDF

Info

Publication number
TWI799778B
TWI799778B TW110100439A TW110100439A TWI799778B TW I799778 B TWI799778 B TW I799778B TW 110100439 A TW110100439 A TW 110100439A TW 110100439 A TW110100439 A TW 110100439A TW I799778 B TWI799778 B TW I799778B
Authority
TW
Taiwan
Prior art keywords
sugar chains
producing same
containing antibodies
antibody population
right asymmetric
Prior art date
Application number
TW110100439A
Other languages
English (en)
Other versions
TW202140541A (zh
Inventor
眞鍋史乃
山口芳樹
岩本将吾
木下崇司
Original Assignee
國立研究開發法人理化學研究所
日商伏見製藥所股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 國立研究開發法人理化學研究所, 日商伏見製藥所股份有限公司 filed Critical 國立研究開發法人理化學研究所
Publication of TW202140541A publication Critical patent/TW202140541A/zh
Application granted granted Critical
Publication of TWI799778B publication Critical patent/TWI799778B/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
TW110100439A 2020-01-10 2021-01-06 均一含有具有左右非對稱之糖鏈之抗體之抗體群、及其製造方法 TWI799778B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2020002968 2020-01-10
JP2020-002968 2020-01-10

Publications (2)

Publication Number Publication Date
TW202140541A TW202140541A (zh) 2021-11-01
TWI799778B true TWI799778B (zh) 2023-04-21

Family

ID=76787974

Family Applications (1)

Application Number Title Priority Date Filing Date
TW110100439A TWI799778B (zh) 2020-01-10 2021-01-06 均一含有具有左右非對稱之糖鏈之抗體之抗體群、及其製造方法

Country Status (6)

Country Link
US (1) US20230039189A1 (zh)
EP (1) EP4089110A4 (zh)
JP (1) JPWO2021140981A1 (zh)
AU (1) AU2020420236A1 (zh)
TW (1) TWI799778B (zh)
WO (1) WO2021140981A1 (zh)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW201309330A (zh) * 2011-01-28 2013-03-01 Abbott Lab 包含糖基化抗體之組合物及其用途
CN104220603A (zh) * 2012-02-10 2014-12-17 马里兰大学，巴尔的摩 抗体及其Fc片段的化学酶法糖基化工程
CN105555801A (zh) * 2013-09-18 2016-05-04 东曹株式会社 Fc结合性蛋白质、该蛋白质的制造方法和使用了该蛋白质的抗体吸附剂、以及使用了该吸附剂的抗体的纯化方法及识别方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015041303A1 (ja) * 2013-09-18 2015-03-26 東ソー株式会社 Ｆｃ結合性タンパク質、当該タンパク質の製造方法および当該タンパク質を用いた抗体吸着剤、ならびに当該吸着剤を用いた抗体の精製方法および識別方法
SG10201914047RA (en) 2015-07-16 2020-03-30 Daiichi Sankyo Co Ltd Novel endos mutant enzyme
JP2020002968A (ja) 2018-06-26 2020-01-09 株式会社Fts 逆止弁およびその取付機構

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW201309330A (zh) * 2011-01-28 2013-03-01 Abbott Lab 包含糖基化抗體之組合物及其用途
CN104220603A (zh) * 2012-02-10 2014-12-17 马里兰大学，巴尔的摩 抗体及其Fc片段的化学酶法糖基化工程
CN105555801A (zh) * 2013-09-18 2016-05-04 东曹株式会社 Fc结合性蛋白质、该蛋白质的制造方法和使用了该蛋白质的抗体吸附剂、以及使用了该吸附剂的抗体的纯化方法及识别方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
期刊 2、 K Masuda et al. Pairing of oligosaccharides in the Fc region of immunoglobulin G. FEBS Lett. 473(3). 2000 May 19. 349-357.;期刊 Shino Manabe et al. Characterization of Antibody Products Obtained through Enzymatic and Nonenzymatic Glycosylation Reactions with a Glycan Oxazoline and Preparation of a Homogeneous Antibody-Drug Conjugate via Fc N-Glycan. Bioconjug Chem. 30(5). Epub 2019 Apr 17. 1343-1355. *
期刊 Shino Manabe et al. Characterization of Antibody Products Obtained through Enzymatic and Nonenzymatic Glycosylation Reactions with a Glycan Oxazoline and Preparation of a Homogeneous Antibody-Drug Conjugate via Fc N-Glycan. Bioconjug Chem. 30(5). Epub 2019 Apr 17. 1343-1355.

Also Published As

Publication number Publication date
EP4089110A1 (en) 2022-11-16
JPWO2021140981A1 (zh) 2021-07-15
US20230039189A1 (en) 2023-02-09
TW202140541A (zh) 2021-11-01
WO2021140981A1 (ja) 2021-07-15
AU2020420236A1 (en) 2022-09-01
EP4089110A4 (en) 2024-01-24

Similar Documents

Publication Publication Date Title
EP3875479A4 (en) AN AGAINST CD19 AND CD3 HOMODIMER-LIKE BISPECIFIC ANTIBODY AND METHOD OF PRODUCTION THEREOF AND USE THEREOF
EP3712178A4 (en) ANTI-HER2 ANTIBODIES OR ANTIGIBODY FRAGMENT THEREOF AND CHIMERAIR ANTIGEN RECEPTOR WITH IT
SG11202100987RA (en) Antibody constructs for cldn18.2 and cd3
ES2523194T3 (es) Anticuerpo monoclonal humano específico para cada tumor
CY1119717T1 (el) Παραγωγη δεσμευτικων μοριων
EP3882268A4 (en) ANTI-CD73 ANTIBODY, ANTIGEN-BINDING FRAGMENT THEREOF AND APPLICATION THEREOF
NO20071436L (no) Humaniserte Anti-5T4 antistoffer og Anti-5T4 antistoff/calicheamicin konjugater
RS52332B (en) HUMAN ANTI-NGF NEUTRAL ANTIBODIES AS SELECTIVE NGF SIGNAL ROUTE INHIBITORS
EA201890928A1 (ru) Антигенсвязывающие белки, которые активируют лептиновый рецептор
BR112013027420A2 (pt) "uso de camundongos geneticamente modificados na preparação de um anticorpo e método para produzir anticorpos".
ATE450549T1 (de) Monoklonale antikörper mit spezifität für fetale erythroidzellen
WO2015109131A3 (en) Bi-specific cd3 and cd19 antigen-binding constructs
ECSP078026A (es) Anticuerpos anti-cd40 humanizados y sus métodos de uso
EA200900492A1 (ru) Молекулы антител, которые связываются с il-17a и il-17f
NO20063767L (no) Antistoffer mot clostridium difficile toksiner og anvendelser derav
BR112013013573A2 (pt) método para produzir anticorpos específicos para um antígeno de interesse, célula b isolada ou recombinante, população de anticorpo, e, anticorpo
EP3851455A4 (en) MONOCLONAL ANTIBODIES SPECIFICALLY BINDING TO HUMAN AND MONKEY ANTIGEN CD38, PROCESS FOR PREPARATION AND USE
WO2007063415A3 (en) Methods of producing stable b-lymphocytes
EA202192907A1 (ru) Моноклональное антитело, которое специфически связывается с gitr
EP3868783A4 (en) ANTI-L1CAM ANTIBODY OR ANTIGEN-BINDING FRAGMENT THEREOF AND CHIMERIC ANTIGEN RECEPTOR THEREOF
EP3981793A4 (en) CEACAM5-RESISTANT MONOCLONAL ANTIBODY AND METHODS FOR ITS PRODUCTION AND ITS USE
MX2021006930A (es) Construcciones de proteínas de fusión para enfermedades asociadas al complemento.
BR112021011529A2 (pt) Método para produção de um anticorpo heterodimérico, e, anticorpo biespecífico isolado
TWI800483B (zh) 抗人類血紅素單株抗體或抗體套組、抗人類血紅素單株抗體固定化不可溶性載體粒子、及使用其等之測定試劑或測定方法
TWI799778B (zh) 均一含有具有左右非對稱之糖鏈之抗體之抗體群、及其製造方法