TWI799778B - 均一含有具有左右非對稱之糖鏈之抗體之抗體群、及其製造方法 - Google Patents

均一含有具有左右非對稱之糖鏈之抗體之抗體群、及其製造方法 Download PDF

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Publication number
TWI799778B
TWI799778B TW110100439A TW110100439A TWI799778B TW I799778 B TWI799778 B TW I799778B TW 110100439 A TW110100439 A TW 110100439A TW 110100439 A TW110100439 A TW 110100439A TW I799778 B TWI799778 B TW I799778B
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TW
Taiwan
Prior art keywords
sugar chains
producing same
containing antibodies
antibody population
right asymmetric
Prior art date
Application number
TW110100439A
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English (en)
Other versions
TW202140541A (zh
Inventor
眞鍋史乃
山口芳樹
岩本将吾
木下崇司
Original Assignee
國立研究開發法人理化學研究所
日商伏見製藥所股份有限公司
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Publication of TW202140541A publication Critical patent/TW202140541A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
TW110100439A 2020-01-10 2021-01-06 均一含有具有左右非對稱之糖鏈之抗體之抗體群、及其製造方法 TWI799778B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2020-002968 2020-01-10
JP2020002968 2020-01-10

Publications (2)

Publication Number Publication Date
TW202140541A TW202140541A (zh) 2021-11-01
TWI799778B true TWI799778B (zh) 2023-04-21

Family

ID=76787974

Family Applications (1)

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TW110100439A TWI799778B (zh) 2020-01-10 2021-01-06 均一含有具有左右非對稱之糖鏈之抗體之抗體群、及其製造方法

Country Status (6)

Country Link
US (1) US20230039189A1 (zh)
EP (1) EP4089110A4 (zh)
JP (1) JPWO2021140981A1 (zh)
AU (1) AU2020420236A1 (zh)
TW (1) TWI799778B (zh)
WO (1) WO2021140981A1 (zh)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW201309330A (zh) * 2011-01-28 2013-03-01 Abbott Lab 包含糖基化抗體之組合物及其用途
CN104220603A (zh) * 2012-02-10 2014-12-17 马里兰大学，巴尔的摩 抗体及其Fc片段的化学酶法糖基化工程
CN105555801A (zh) * 2013-09-18 2016-05-04 东曹株式会社 Fc结合性蛋白质、该蛋白质的制造方法和使用了该蛋白质的抗体吸附剂、以及使用了该吸附剂的抗体的纯化方法及识别方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015041303A1 (ja) * 2013-09-18 2015-03-26 東ソー株式会社 Ｆｃ結合性タンパク質、当該タンパク質の製造方法および当該タンパク質を用いた抗体吸着剤、ならびに当該吸着剤を用いた抗体の精製方法および識別方法
WO2017010559A1 (ja) 2015-07-16 2017-01-19 第一三共株式会社 新規ＥｎｄｏＳ変異酵素
JP2020002968A (ja) 2018-06-26 2020-01-09 株式会社Fts 逆止弁およびその取付機構

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW201309330A (zh) * 2011-01-28 2013-03-01 Abbott Lab 包含糖基化抗體之組合物及其用途
CN104220603A (zh) * 2012-02-10 2014-12-17 马里兰大学，巴尔的摩 抗体及其Fc片段的化学酶法糖基化工程
CN105555801A (zh) * 2013-09-18 2016-05-04 东曹株式会社 Fc结合性蛋白质、该蛋白质的制造方法和使用了该蛋白质的抗体吸附剂、以及使用了该吸附剂的抗体的纯化方法及识别方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
期刊 2、 K Masuda et al. Pairing of oligosaccharides in the Fc region of immunoglobulin G. FEBS Lett. 473(3). 2000 May 19. 349-357.;期刊 Shino Manabe et al. Characterization of Antibody Products Obtained through Enzymatic and Nonenzymatic Glycosylation Reactions with a Glycan Oxazoline and Preparation of a Homogeneous Antibody-Drug Conjugate via Fc N-Glycan. Bioconjug Chem. 30(5). Epub 2019 Apr 17. 1343-1355. *
期刊 Shino Manabe et al. Characterization of Antibody Products Obtained through Enzymatic and Nonenzymatic Glycosylation Reactions with a Glycan Oxazoline and Preparation of a Homogeneous Antibody-Drug Conjugate via Fc N-Glycan. Bioconjug Chem. 30(5). Epub 2019 Apr 17. 1343-1355.

Also Published As

Publication number Publication date
WO2021140981A1 (ja) 2021-07-15
TW202140541A (zh) 2021-11-01
EP4089110A1 (en) 2022-11-16
US20230039189A1 (en) 2023-02-09
AU2020420236A1 (en) 2022-09-01
EP4089110A4 (en) 2024-01-24
JPWO2021140981A1 (zh) 2021-07-15

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