TWI775420B - A method of fertilizer application - Google Patents

A method of fertilizer application Download PDF

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TWI775420B
TWI775420B TW110116245A TW110116245A TWI775420B TW I775420 B TWI775420 B TW I775420B TW 110116245 A TW110116245 A TW 110116245A TW 110116245 A TW110116245 A TW 110116245A TW I775420 B TWI775420 B TW I775420B
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ingredients
enzymes
fertilizer
prepared
fertilizers
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TW202243584A (en
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楊秋忠
楊禮亘
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地天泰農業生技股份有限公司
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Abstract

The present invention provides a method of fertilizer application, composing the following steps: respectively applying an enzyme to multiple different types of fertilizer material to obtain multiple different types of ingredients; mixing the ingredients in different proportions to obtain multiple different types of complex fertilizers; respectively applying the complex fertilizers to soil to obtain multiple different types of soil samples; analyzing the soil samples to obtain the result of analyzation disclosing the nutrition contents of those soil samples; comparing those nutrition contents with a crop need for applying fertilizers, and selecting a subject complex fertilizer from multiple different types of the complex fertilizers; and applying the subject complex fertilizer to the crop.

Description

施肥方法Fertilization method

本揭露關於一種施予肥料的方法。更具體來說,本揭露關於一種可精準得知所施予肥料之養分量之施予肥料的方法。The present disclosure pertains to a method of applying fertilizer. More specifically, the present disclosure relates to a method of applying fertilizer which can accurately know the nutrient amount of the applied fertilizer.

習知有機質肥料的依據生產製程可分為兩大類:經過微生物發酵的有機質肥料以及未經過微生物發酵的有機質肥料。所謂經過微生物發酵製程所得的肥料指的就是經過堆肥法獲得的肥料。另一方面,所謂未經過微生物發酵製程所得的肥料指的則是乾燥的動物、植物或微生物的殘體。The known production process of organic fertilizers can be divided into two categories: organic fertilizers that have undergone microbial fermentation and organic fertilizers that have not undergone microbial fermentation. The so-called fertilizer obtained through the microbial fermentation process refers to the fertilizer obtained through the composting method. On the other hand, the so-called fertilizer obtained without microbial fermentation process refers to the residues of dried animals, plants or microorganisms.

具體來說傳統堆肥法是透過將原料與泥土混合,藉由泥土中的各種微生物所分泌的酵素來分解原料而成為養分。在這過程中,微生物利用有機物來獲得氮源、磷源及碳源等能源,並釋放出二氧化碳或甲烷等溫室氣體。然而,傳統的堆肥法沒有特定的酵素控制系統,一般來說需要花費2至4個月的時間,而非常耗時。況且,肥料堆置需要很大的空間,因而沒有充分地有效利用空間。再者,肥料製備過程中可能會產生惡臭氣體與髒水,而會影響環境整潔。此外,傳統堆肥中的微生物及其酵素活性都會受到外在環境中的溼度與溫度影響,因此不同季節以堆肥法所生產出的肥料,其腐熟程度差異很大,而無法精準控制品質。Specifically, the traditional composting method is to decompose the raw materials into nutrients by mixing raw materials with soil, and using enzymes secreted by various microorganisms in the soil to decompose the raw materials. In this process, microorganisms use organic matter to obtain energy sources such as nitrogen, phosphorus and carbon sources, and release greenhouse gases such as carbon dioxide or methane. However, the traditional composting method does not have a specific enzyme control system, and it generally takes 2 to 4 months, which is very time-consuming. Moreover, the composting requires a lot of space, so the space is not fully utilized efficiently. Furthermore, odorous gas and dirty water may be generated during the fertilizer preparation process, which will affect the cleanliness of the environment. In addition, the microorganisms and their enzyme activities in traditional composting are affected by the humidity and temperature in the external environment. Therefore, fertilizers produced by the composting method in different seasons have great differences in the degree of decomposing, and the quality cannot be precisely controlled.

此外,習知的有機質肥料不論是經過微生物發酵或是未經過微生物發酵,都難以精準掌握肥料釋出其中養分的釋放量或釋放時間。因此,施予有機質肥料後,往往在作物的生長期間還要「追肥」,也就是追加補充肥料,才能足以滿足作物在生長期各個階段中的養分需求。但是追肥極為耗工,在施予肥料前還要時時掌握土壤中的養分含量,才能評估需要施予的特定養分的肥料以及施予肥料的時間點,以達到適時、適量、適地對作物補充肥料。In addition, whether conventional organic fertilizers are microbially fermented or not microbially fermented, it is difficult to accurately grasp the release amount or release time of nutrients in the fertilizer. Therefore, after the application of organic fertilizers, "top dressing" is often required during the growth period of the crops, that is, supplementary fertilizers are added to meet the nutrient needs of the crops in all stages of the growth period. However, top dressing is extremely labor-intensive. Before applying fertilizers, it is necessary to constantly grasp the nutrient content in the soil, in order to evaluate the specific nutrient fertilizers that need to be applied and the time point of applying fertilizers, so as to achieve timely, appropriate and appropriate supplementation to crops. fertilizer.

現今施肥方法除了會面臨人力、時間成本的壓力外,也還要考量作物在生長期各階段中的養分需求。因此,此領域仍有開發更精準地施予肥料的方法之期待。In addition to the pressure of manpower and time costs, the current fertilization methods also need to consider the nutrient requirements of crops in various stages of the growth period. Therefore, there is still hope in the field to develop methods for more precise application of fertilizers.

有鑑於上述問題,本揭露之目的係提供一種有別於傳統之施肥方法,其施肥方法較習知方法更為精準地調整肥料中養分的釋放量與釋放時間。In view of the above problems, the purpose of the present disclosure is to provide a fertilization method which is different from the traditional fertilization method, and the fertilization method can adjust the release amount and release time of nutrients in the fertilizer more accurately than the conventional method.

為達上述目的,本揭露提供一種施肥方法,其包含下列步驟:複數種類的製肥原料分別施予一酵素,以製得複數個配料;該些配料以不同比例混合,以製得複數種類的複合肥料;將該些複合肥料各自加入土壤,以得複數個土壤樣品;檢測該些土壤樣品,以得一檢測結果,其揭露該些土壤樣品之養分含量;根據一作物的施肥需求比對該些養分含量,以從複數種類的複合肥料中選擇一標的複合肥料;及對該作物施予該標的複合肥料。In order to achieve the above object, the present disclosure provides a fertilization method, which includes the following steps: applying an enzyme to a plurality of types of fertilizer raw materials to obtain a plurality of ingredients; mixing the ingredients in different proportions to obtain a plurality of types of compound fertilizers; adding these compound fertilizers to soil respectively to obtain a plurality of soil samples; testing the soil samples to obtain a test result, which discloses the nutrient content of the soil samples; comparing the soil samples according to the fertilization requirements of a crop to select a target compound fertilizer from a plurality of types of compound fertilizers; and apply the target compound fertilizer to the crop.

較佳地,該製肥原料為生物質。Preferably, the fertilizer raw material is biomass.

較佳地,該生物質為雞糞、牛糞或真菌類培養用木屑。Preferably, the biomass is chicken manure, cow manure or wood chips for fungal culture.

較佳地,該酵素為分解酵素(hydrolases)、合成酵素(synthetases)、轉化酵素(transferases)、聚合酵素(polymerases)或前述酵素兩種以上之組合。Preferably, the enzymes are hydrolases, synthetases, transferases, polymerases, or a combination of two or more of the foregoing enzymes.

較佳地,該酵素為分解酵素、合成酵素、轉化酵素及聚合酵素之混合物,其混合比例以重量比計,為1~5:1~5:1~5:1~5。Preferably, the enzyme is a mixture of decomposing enzyme, synthetic enzyme, inverting enzyme and polymerizing enzyme, and the mixing ratio is 1-5:1-5:1-5:1-5 in terms of weight ratio.

較佳地,該些複合肥料係以雞糞製得之配料與以牛糞製得之配料,各自以介於1:2~3:1之重量比混合而成。Preferably, the compound fertilizers are prepared by mixing the ingredients obtained from chicken manure and the ingredients obtained from cow manure in a weight ratio of 1:2 to 3:1.

較佳地,該些複合肥料係以雞糞製得之配料與以真菌類培養用木屑製得之配料,各自以介於1:2~3:1之重量比混合而成。Preferably, the compound fertilizers are prepared by mixing the ingredients prepared from chicken manure and the ingredients prepared from wood chips for fungal culture in a weight ratio of 1:2 to 3:1.

較佳地,該些複合肥料係以牛糞製得之配料與以真菌類培養用木屑製得之配料,各自以介於1:2~3:1之重量比混合而成。Preferably, the compound fertilizers are prepared by mixing the ingredients prepared from cow dung and the ingredients prepared from wood chips for fungal culture in a weight ratio of 1:2 to 3:1.

較佳地,該些複合肥料係以雞糞製得之配料、以牛糞製得之配料與以真菌類培養用木屑製得之配料,各自以介於1~6:1~3:1~3之重量比混合而成。Preferably, these compound fertilizers are ingredients prepared from chicken manure, ingredients prepared from cow dung, and ingredients prepared from wood chips for fungal culture, each in a ratio of 1-6:1-3:1-3. The weight ratio is mixed.

較佳地,該養分為氮、磷、鉀或前述養分兩種以上之組合。Preferably, the nutrient is nitrogen, phosphorus, potassium or a combination of two or more of the foregoing nutrients.

較佳地,該作物為果樹類或蔬菜類。Preferably, the crops are fruit trees or vegetables.

本揭露之施肥方法藉由檢測以不同比例混合配料(即單一肥料)所完成的複數種複合肥料於土壤中所釋放出養分種類及養分含量,可明確得知該些複合肥料於單位時間內所釋放出之養分含量,並依據目標作物之施肥需求及該檢測結果,選擇目標作物所適合的複合肥料進行施肥,可使本揭露之施肥方法較為傳統方法更為精準地調整肥料中養分的釋放量與釋放時間。In the fertilization method of the present disclosure, by detecting the types and contents of nutrients released in the soil by a plurality of compound fertilizers prepared by mixing ingredients in different proportions (ie, a single fertilizer), it can be clearly known that the compound fertilizers are used in a unit time. The released nutrient content, and according to the fertilization requirements of the target crop and the test results, the compound fertilizer suitable for the target crop is selected for fertilization, so that the fertilization method disclosed in the present disclosure can more accurately adjust the release amount of nutrients in the fertilizer compared with the traditional method. and release time.

本揭露所使用的全部技術和科學名詞與本發明所屬技術領域中具有通常知識者通常所瞭解的意義相同,除非本揭露中另有說明。All technical and scientific terms used in the present disclosure have the same meanings as commonly understood by those of ordinary skill in the technical field to which the present invention belongs, unless otherwise specified in the present disclosure.

本揭露為了更精準地提供作物所需要的養分,研發出一種施肥方法,其包含下列步驟:複數種類的製肥原料分別施予一酵素,以製得複數個配料;該些配料以不同比例混合,以製得複數種類的複合肥料;將該些複合肥料各自加入土壤,以得複數個土壤樣品;檢測該些土壤樣品,以得一檢測結果,其揭露該些土壤樣品之養分含量;根據一作物的施肥需求比對該些養分含量,以從複數種類的複合肥料中選擇一標的複合肥料;及對該作物施予該標的複合肥料。In order to provide the nutrients required by crops more accurately, the present disclosure develops a fertilization method, which includes the following steps: applying an enzyme to a plurality of types of fertilizer raw materials to prepare a plurality of ingredients; mixing the ingredients in different proportions , to prepare multiple types of compound fertilizers; adding these compound fertilizers to soil respectively to obtain multiple soil samples; testing the soil samples to obtain a test result, which discloses the nutrient content of the soil samples; according to a The fertilization requirements of crops are compared to these nutrient contents to select a target compound fertilizer from a plurality of types of compound fertilizers; and the target compound fertilizer is applied to the crop.

本揭露所使用的製肥原料在製成配料(單一肥料)後能釋放養分之下,並未特別限定其來源、種類。在一可行的態樣中,製肥原料為取自生物體的有機物質,即所謂的生物質。在一可行的態樣中,製肥原料為以化學合成方法由非生物體或無機化合物、礦物中所提煉製成。在一可行的態樣中,作為該生物質來源的生物體為動物、植物或微生物。在一可行的態樣中,該生物體為雞、鴨、鵝、火雞、鴕鳥、鴿子等家禽類;牛、豬、鹿、兔、羊、馬、驢等家畜類;魚、蝦、貝等水產類。在一可行的態樣中,該生物質為生物體、或其排泄物、排遺物、養殖過程中所產生的廢棄物。在一可行的態樣中,該生物體為甘蔗、甜菜、玉米、小麥、高粱、大豆、木薯、水稻、芒草、樹木、水藻。在一具體的態樣中,該生物質為雞糞、牛糞或真菌類培養用木屑。在一可行的態樣中,該生物質所包含的組成成分有蛋白質、碳水化合物(單糖、雙糖、半纖維素、纖維素)、木質素、脂質和臘等。The fertilizer raw materials used in the present disclosure can release nutrients after being made into ingredients (single fertilizers), and their sources and types are not particularly limited. In one possible aspect, the feedstock for fertilizer production is organic matter obtained from living organisms, so-called biomass. In a feasible aspect, the fertilizer raw materials are extracted from non-living or inorganic compounds and minerals by chemical synthesis methods. In a possible aspect, the organism from which the biomass is derived is an animal, plant or microorganism. In a feasible aspect, the organism is poultry such as chicken, duck, goose, turkey, ostrich, pigeon; livestock such as cattle, pig, deer, rabbit, sheep, horse, donkey; fish, shrimp, shellfish, etc. and other aquatic products. In a feasible aspect, the biomass is an organism, or its excrement, excreta, or waste generated during aquaculture. In one possible aspect, the organism is sugar cane, sugar beet, corn, wheat, sorghum, soybean, cassava, rice, miscanthus, tree, algae. In a specific aspect, the biomass is chicken manure, cow manure or wood chips for fungal culture. In a feasible aspect, the biomass contains constituents such as proteins, carbohydrates (monosaccharides, disaccharides, hemicelluloses, cellulose), lignin, lipids and waxes.

傳統堆肥法是直接將原料與土壤混合,需要長時間、密集勞力及大空間,且外在環境條件影響土壤中微生物的生長情況,使肥料的品質難以掌控。為了降低人工成本、縮短製程時間、製備品質穩定的肥料,本揭露採用對製肥原料施予酵素的方式來製成肥料。本揭露所使用的酵素若能發揮效果,則並未特別限定種類。在一可行的態樣中,酵素為分解酵素(hydrolases)、合成酵素(synthetases)、轉化酵素(transferases)、聚合酵素(polymerases)或前述酵素兩種以上(諸如:兩種、三種或四種)之組合。在一具體的態樣中,分解酵素為蛋白質分解酵素、脂肪分解酵素、纖維分解酵素、澱粉分解酵素、木質素分解酵素或前述酵素兩種以上(諸如:兩種、三種、四種或五種)之組合。在一具體的態樣中,合成酵素為琥珀醯輔酶(Asuccinyl-CoA)合成酵素、麩胺醯胺合成酶(glutamine synthetase)、高羧酸酶合成酶(Holocarboxylase synthetase;HCS)、四異戊二烯焦磷酸合成酶(geranylgeranyl pyrophosphate synthase)、腐植化酵素(humification enzyme)或前述酵素兩種以上(諸如:兩種、三種、四種或五種)之組合。在一具體的態樣中,轉化酵素為GGPP(Geranylgeranyl Diphosphate transferase)、穀胱甘肽S-轉化酵素(glutathione S-transferase,GST)、羥基肉桂醯基轉化酵素(hydroxycinnamoyl transferase,HCT)、異戊二烯轉移酶(trans-prenyl transferase)、氨基轉移酶(amino transferase)或前述酵素兩種以上(諸如:兩種、三種、四種或五種)之組合。在一具體的態樣中,聚合酵素為脫氫聚合酵素(dehydrogenation polymerase)。The traditional composting method is to directly mix the raw materials with the soil, which requires a long time, intensive labor and large space, and the external environmental conditions affect the growth of microorganisms in the soil, making it difficult to control the quality of the fertilizer. In order to reduce labor costs, shorten the process time, and prepare fertilizers with stable quality, the present disclosure adopts the method of applying enzymes to fertilizer-making raw materials to prepare fertilizers. The enzymes used in the present disclosure are not particularly limited as long as they are effective. In one possible form, the enzymes are hydrolases, synthetases, transferases, polymerases, or two or more of the foregoing (such as two, three, or four) combination. In a specific aspect, the lytic enzyme is a proteolytic enzyme, a lipolytic enzyme, a cellulolytic enzyme, an amylolytic enzyme, a lignin-lytic enzyme, or two or more of the foregoing enzymes (such as two, three, four, or five) ) combination. In a specific aspect, the synthetic enzymes are Asuccinyl-CoA synthetase, glutamine synthetase, Holocarboxylase synthetase (HCS), tetraisopentane geranylgeranyl pyrophosphate synthase, humification enzyme, or a combination of two or more of the aforementioned enzymes (such as two, three, four or five). In a specific aspect, the converting enzymes are GGPP (Geranylgeranyl Diphosphate transferase), glutathione S-transferase (GST), hydroxycinnamoyl transferase (HCT), isoprene Diene transferase (trans-prenyl transferase), amino transferase (amino transferase) or a combination of two or more of the aforementioned enzymes (such as: two, three, four or five). In a specific aspect, the polymerase is a dehydrogenation polymerase.

於一較佳實施態樣中,添加於製肥原料中的酵素係分解酵素、合成酵素、轉化酵素及聚合酵素之混合物。在一可行的態樣中,分解酵素、合成酵素、轉化酵素及聚合酵素的比例以重量計為1~5:1~5:1~5:1~5中的各整數比。在一具體的態樣中,分解酵素、合成酵素、轉化酵素及聚合酵素的比例以重量計為1:1:1:1、2:1:1:1、1:2:1:1、1:1:2:1或1:1:1:2。在一具體的態樣中,分解酵素、合成酵素、轉化酵素及聚合酵素的比例以重量計為2:2:1:1、1:2:2:1、1:1:2:2或2:1:1:2。在一具體的態樣中,分解酵素、合成酵素、轉化酵素及聚合酵素的比例以重量計為2:2:2:1、1:2:2:2、2:1:2:2或2:2:1:2。In a preferred embodiment, the enzymes added to the fertilizer raw material are a mixture of decomposing enzymes, synthetic enzymes, inverting enzymes and polymerizing enzymes. In a practicable aspect, the ratio of the catabolic enzyme, the synthetic enzyme, the inverting enzyme and the polymerase is an integer ratio of 1-5:1-5:1-5:1-5 by weight. In a specific aspect, the ratio of catabolic enzyme, synthetic enzyme, inverting enzyme and polymerase is 1:1:1:1, 2:1:1:1, 1:2:1:1, 1 by weight :1:2:1 or 1:1:1:2. In a specific aspect, the ratio of lytic enzyme, synthetic enzyme, inverting enzyme and polymerase is 2:2:1:1, 1:2:2:1, 1:1:2:2 or 2 by weight :1:1:2. In a specific aspect, the ratio of lytic enzyme, synthetic enzyme, inverting enzyme and polymerase is 2:2:2:1, 1:2:2:2, 2:1:2:2 or 2 by weight :2:1:2.

對製肥原料施予酵素的製程係可因應需求來控制其條件。在一可行的態樣中,對製肥原料添加酵素後,以攪拌機將兩者充分混合。在一可行的態樣中,對製肥原料添加酵素並混合兩者的製程總時間為1、1.5、2、2.5、3或3.5小時。在一可行的態樣中,攪拌機持續性地混合製肥原料與酵素的同時,加溫達到60、70、75、80、85或90℃。在一可行的態樣中,對製肥原料施予酵素並混合兩者的製程的濕度為35、40、45、50、55、60、65、70、75或80%。在一可行的態樣中,對製肥原料施予酵素並混合兩者的製程的酸鹼值(pH)為6.0、6.2、6.4、6.6、6.8、7.0、7.2、7.4、7.6、7.8、7.9或8.0。將複數種類的製肥原料各自與酵素充分混合完成後(如有需要,可再進行滅菌處理),即可獲得複數個配料。The process of applying enzymes to fertilizer raw materials can be controlled according to needs. In a feasible aspect, after adding enzymes to the fertilizer raw materials, the two are thoroughly mixed with a mixer. In one possible aspect, the total process time for adding the enzymes to the fertilizer raw material and mixing the two is 1, 1.5, 2, 2.5, 3, or 3.5 hours. In a feasible aspect, the mixer continuously mixes the fertilizer raw material and the enzyme while heating to 60, 70, 75, 80, 85 or 90°C. In one possible aspect, the humidity of the process of applying the enzymes to the fertilizer feedstock and mixing the two is 35, 40, 45, 50, 55, 60, 65, 70, 75 or 80%. In a possible aspect, the pH of the process of applying the enzymes to the fertilizer raw material and mixing the two is 6.0, 6.2, 6.4, 6.6, 6.8, 7.0, 7.2, 7.4, 7.6, 7.8, 7.9 or 8.0. After fully mixing multiple types of fertilizer raw materials with enzymes (and sterilizing if necessary), multiple ingredients can be obtained.

對於該些配料分別檢測其所含的養分種類與含量。配料中養分種類與含量的檢測方法包含下列步驟:取配料樣品加入去離子水浸泡,接著震盪加熱,經濾紙過濾,將該過濾後的液體經分析法分析其中的養分種類及其含量。在一可行的態樣中,分析配料樣品中的養分種類是氮(N)、磷(P)、鉀(K)、鈣(Ca)、鎂(Mg)、硫(S)、鐵(Fe)、錳(Mn)、硼(B)、銅(Cu)、鉬(Mo)、鋅(Zn)、氯(Cl)或前述養分兩種以上(諸如:兩種、三種、四種、五種)之組合。在一較佳的態樣中,分析配料樣品中的養分種類是氮(N)、磷(P)、鉀(K)或前述養分兩種以上(諸如:兩種、三種)之組合。在一可行的態樣中,分析法為NIEA W423.52C 分析法或ICP分析法。根據配料中養分的檢測結果,可獲得最適用於製造肥料之酵素。當酵素為兩種以上之酵素組合使用時,可根據配料中養分的檢測結果,獲得最佳之酵素比例。The types and contents of nutrients contained in these ingredients were tested respectively. The detection method for the types and contents of nutrients in ingredients includes the following steps: taking ingredients samples and soaking them in deionized water, then oscillating heating, filtering through filter paper, and analyzing the nutrient types and contents in the filtered liquid by analytical method. In a practical aspect, the nutrient species in the analysis ingredient samples are nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), iron (Fe) , manganese (Mn), boron (B), copper (Cu), molybdenum (Mo), zinc (Zn), chlorine (Cl) or two or more of the aforementioned nutrients (such as: two, three, four, five) combination. In a preferred aspect, the nutrient species in the analysis ingredient sample are nitrogen (N), phosphorus (P), potassium (K), or a combination of two or more of the foregoing nutrients (such as two or three). In a possible aspect, the analytical method is NIEA W423.52C analytical method or ICP analytical method. According to the test results of the nutrients in the ingredients, the most suitable enzymes for making fertilizers can be obtained. When two or more enzymes are used in combination, the optimal ratio of enzymes can be obtained according to the test results of nutrients in the ingredients.

將土壤添加於該些配料中,於一定時取得土壤樣品。針對該土壤樣品進行檢測,以獲得各種配料於不同時間點所釋放的養分種類與含量,作為混合各種配料的比例的參考。在一可行的態樣中,對土壤樣品的檢測方法,採用與配料的檢測方法相同的方法。Soil was added to these ingredients and soil samples were taken at certain times. The soil samples were tested to obtain the types and contents of nutrients released by various ingredients at different time points, which were used as a reference for the ratio of mixing various ingredients. In a practicable form, the testing method for soil samples is the same as the testing method for ingredients.

將該些配料以不同比例混合,以製得複數種類的複合肥料。在一可行態樣中,配料為以雞糞製得之配料、以牛糞製得之配料或以真菌類培養用木屑製得之配料。在一可行的態樣中,複合肥料係以雞糞製得之配料與以真菌類培養用木屑製得之配料,以重量計為1:2~3:1中的各整數比(諸如:1:2、1:3、2:3)混合而得。在一可行的態樣中,複合肥料係以牛糞製得之配料與以真菌類培養用木屑製得之配料,以重量計為1:2~3:1中的各整數比(諸如:1:2、1:3、2:3)混合而得。在一較佳的態樣中,該些複合肥料係以雞糞製得之配料與以牛糞製得之配料,以重量計為1:2~3:1中的各整數比(諸如:1:2、1:3、2:3)混合而得。在一可行的態樣中,複合肥料係以雞糞製得之配料、以牛糞製得之配料與以真菌類培養用木屑製得之配料,以重量計為1~6:1~3:1~3中的各整數比(諸如:2:2:3、3:2:2、4:2:3、5:2:3)混合而得。These ingredients are mixed in different proportions to make multiple types of compound fertilizers. In a possible aspect, the ingredients are ingredients made from chicken manure, ingredients made from cow dung, or ingredients made from wood chips for fungal culture. In a feasible aspect, the compound fertilizer is the ingredient prepared from chicken manure and the ingredient prepared from wood chips for fungal culture, in an integer ratio of 1:2 to 3:1 by weight (such as: 1 : 2, 1: 3, 2: 3) mixed. In a feasible aspect, the compound fertilizer is the ingredient made from cow dung and the ingredient made from wood chips for fungal culture, in an integer ratio of 1:2 to 3:1 by weight (such as: 1:1:1). 2, 1:3, 2:3) mixed. In a preferred aspect, the compound fertilizers are the ingredients made from chicken manure and the ingredients made from cow manure, and the ratio of each integer in the range of 1:2 to 3:1 by weight (such as: 1:1:1) 2, 1:3, 2:3) mixed. In a feasible aspect, the compound fertilizer is the ingredient prepared from chicken manure, the ingredient prepared from cow dung, and the ingredient prepared from wood chips for fungal culture, and the ratio is 1-6:1-3:1 by weight The integer ratios in ~3 (such as: 2:2:3, 3:2:2, 4:2:3, 5:2:3) are mixed.

將該些複合肥料各自添加土壤,以取得複數個土壤樣品。定時針對該些土壤樣品進行檢測,以獲得揭露有土壤樣品中養分種類與含量的檢測結果。藉此,可得知該些複合肥料於單位時間內所釋放至土壤樣品中的養分種類與含量。對土壤樣品的檢測方法係可採用與前述肥料的檢測方法相同的方法。舉例而言,為檢測土壤樣品中之氮含量,可使用NIEA W423.52C分析法。相同地,為檢測前述肥料中之氮含量,可使用NIEA W423.52C分析法。These compound fertilizers were each added to the soil to obtain a plurality of soil samples. The soil samples are regularly tested to obtain test results revealing the types and contents of nutrients in the soil samples. Thereby, the types and contents of nutrients released by the compound fertilizers into the soil samples in a unit time can be known. The detection method of the soil sample can be the same as the detection method of the aforementioned fertilizer. For example, to detect nitrogen content in soil samples, the NIEA W423.52C analytical method can be used. Likewise, in order to detect the nitrogen content in the aforementioned fertilizers, the NIEA W423.52C analytical method can be used.

根據一作物的施肥需求比對該些養分含量,以從複數種類的複合肥料中選擇一標的複合肥料。該作物係可為農藝作物、園藝作物、經濟林木,但並不限於此。該作物係可為油料類的大豆或花生;澱粉類的玉米或甘薯;纖維類的棉花或木棉;木材類的橡樹或柚樹;糖料類的甘蔗或糖楓;香料類的胡椒或辣椒;染料類的番紅花或薑黃;樹液類的橡膠樹;觀賞類的花壇植物、棚架植物、水生植物,但不限於此。較佳地,該作物為果樹類或蔬菜類(例如:葉菜類或果菜類)。作物在生長的不同階段對養分種類與含量的需求不同。選定特定作物並了解其各階段所需養分種類與含量後,比對施予複合肥料後的土壤樣品中養分種類的釋放時間與釋放含量的檢測結果,從複數種類的複合肥料中選擇一養分種類釋放時間與釋放含量符合作物養分需求的複合肥料,作為標的複合肥料,來施予該作物。According to the fertilization requirements of a crop, these nutrient contents are compared to select a target compound fertilizer from a plurality of types of compound fertilizers. The crop system can be agronomic crops, horticultural crops, and commercial forest trees, but is not limited thereto. The crop system can be soybean or peanut of oil; corn or sweet potato of starch; cotton or kapok of fiber; oak or teak of wood; sugar cane or maple of sugar; pepper or chili of spice; Dye-type saffron or turmeric; sap-type rubber tree; ornamental-type flower bed plants, trellis plants, aquatic plants, but not limited thereto. Preferably, the crops are fruit trees or vegetables (eg, leafy vegetables or fruit vegetables). Crops require different types and contents of nutrients at different stages of growth. After selecting a specific crop and understanding the types and contents of nutrients required at each stage, compare the release time and release content of the nutrient types in the soil samples after applying compound fertilizers, and select a nutrient type from multiple types of compound fertilizers The compound fertilizer whose release time and release content meet the nutrient requirements of the crop is used as the target compound fertilizer to be applied to the crop.

本揭露之施肥方法藉由檢測以不同比例混合配料所完成的複數種複合肥料於土壤中所釋放出養分種類及養分含量,可明確得知該些複合肥料於單位時間內所釋放出之養分含量,並依據目標作物之施肥需求及該檢測結果,選擇目標作物所適合的複合肥料進行施肥,可使本揭露之施肥方法較為傳統方法更為精準地調整肥料中養分的釋放量與釋放時間。The fertilization method of the present disclosure can clearly know the nutrient content released by the compound fertilizers in a unit time by detecting the types and contents of nutrients released in the soil by a plurality of compound fertilizers prepared by mixing ingredients in different proportions. , and according to the fertilization requirements of the target crops and the detection results, the compound fertilizer suitable for the target crops is selected for fertilization, so that the fertilization method disclosed in the present disclosure can more accurately adjust the release amount and release time of nutrients in the fertilizer compared with the traditional methods.

[實施例][Example]

製肥原料與酵素Fertilizer raw materials and enzymes

本揭露所選用之製肥原料為雞糞、牛糞與以真菌類培養用木屑,其中係以香菇木屑作為以真菌類培養用木屑之實例。選用的理由在於該等種類原料為養分含量(氮、磷、鉀)與分解性較高及較低的代表性原料,具體如表1所示。將上述原料分別調整其中的水分含量為50 %,待與酵素混合。The fertilizer raw materials selected in the present disclosure are chicken manure, cow manure and wood chips for cultivation of fungi, among which the wood chips of Lentinus edodes are used as an example of wood chips for cultivation of fungi. The reason for the selection is that these types of raw materials are representative raw materials with high and low nutrient content (nitrogen, phosphorus, potassium) and decomposability, as shown in Table 1. The above-mentioned raw materials are respectively adjusted to have a moisture content of 50%, and are to be mixed with enzymes.

表1   養分含量 分解性 雞糞 牛糞 香菇木屑 Table 1 Nutrient content Decomposability chicken manure high high cow dung middle middle Mushroom Sawdust Low Low

本揭露選用4種酵素的組合,分別是分解酵素(hydrolases)、合成酵素(synthetases)、轉化酵素(transferases)、聚合酵素(polymerases)。該等酵素的特徵如表2所示。The present disclosure selects a combination of four kinds of enzymes, namely hydrolases, synthetases, transferases, and polymerases. The characteristics of these enzymes are shown in Table 2.

表2 分解酵素 蛋白質(EC 3.4.)、脂肪(EC 3.1.)、醣類(EC 3.2)等的水解酵素 合成酵素 合成C-O(EC 6.1)、C-S(EC 6.2)、C-N (EC 6.3)、C-C (EC 6.4)等鍵接的合成酵素 轉化酵素 轉氨(EC 2.6)及轉甲基(EC 2.1)酵素,其是催化氨基及甲基轉移的一種轉化酵素 聚合酵素 多酚氧化酵素(EC 1.10.3.1)是催化酚類的一種氧化及聚合酵素 Table 2 catalyzing enzymes Hydrolytic enzymes of proteins (EC 3.4.), fats (EC 3.1.), carbohydrates (EC 3.2), etc. synthetic enzymes Synthesis of bonded synthetic enzymes such as CO (EC 6.1), CS (EC 6.2), CN (EC 6.3), CC (EC 6.4) invertase Transamination (EC 2.6) and transmethylation (EC 2.1) enzymes, which are an inverting enzyme that catalyzes the transfer of amino groups and methyl groups polymerase Polyphenol oxidase (EC 1.10.3.1) is an enzyme that catalyzes the oxidation and polymerization of phenols

實施例一:配料的製備Example 1: Preparation of ingredients

將作為製肥原料的雞糞、牛糞與以香菇木屑各秤重1公斤,將原料總量2 %的酵素混合物分別加入至各個製肥原料,並以攪拌機進行攪拌使之混合均勻。前述酵素混合物中的分解酵素(蛋白酶、纖維素酶、及木質素酶中至少一者)、合成酵素(腐植化酵素)、轉化酵素(氨基轉移酶)與聚合酵素(脫氫聚合酶)的比例以重量計,分別為1:1:1:1、2:1:1:1、1:2:1:1、1:1:2:1及1:1:1:2。將混有酵素之製肥原料加溫至80 ℃後,反應2.5小時,再經過30分鐘的滅菌處理,獲得各種製肥原料與不同比例的酵素混合物混合後所製成的各種配料。分別檢測各種配料中氮、磷、鉀的總含量。檢測方法包含下列步驟:取前述配料100公克加入去離子水浸泡,接著以60℃震盪加熱30分鐘,以125 mm sartorius stedim No.393的濾紙過濾。將該過濾後的液體藉由NIEA W423.52C 分析法、ICP分析法、ICP分析法,以分別檢測氮、磷及鉀之含量。各種配料中氮、磷及鉀的總含量檢測結果如表3所示。Chicken dung, cow dung, and shiitake mushroom sawdust, which are the raw materials for fertilizer production, are weighed by 1 kg each, and the enzyme mixture containing 2% of the total raw material is added to each fertilizer raw material, and stirred with a mixer to make it evenly mixed. The ratio of degradative enzymes (at least one of protease, cellulase, and ligninase), synthetic enzymes (humic enzymes), invertases (aminotransferases) and polymerases (dehydrogenases) in the aforementioned enzyme mixture By weight, they are 1:1:1:1, 2:1:1:1, 1:2:1:1, 1:1:2:1 and 1:1:1:2. The fertilizer raw materials mixed with enzymes are heated to 80 ℃, reacted for 2.5 hours, and then sterilized for 30 minutes to obtain various ingredients made by mixing various fertilizer raw materials with different ratios of enzyme mixtures. The total contents of nitrogen, phosphorus and potassium in various ingredients were detected respectively. The detection method includes the following steps: take 100 grams of the aforementioned ingredients and add them to deionized water for soaking, then shake and heat at 60° C. for 30 minutes, and filter with 125 mm sartorius stedim No.393 filter paper. The filtered liquid was analyzed by NIEA W423.52C analysis method, ICP analysis method, and ICP analysis method to detect the contents of nitrogen, phosphorus and potassium, respectively. The test results of the total content of nitrogen, phosphorus and potassium in various ingredients are shown in Table 3.

表3. 各種配料中氮、磷及鉀的總含量檢測結果   製肥原料   酵素比例* 總含量的百分率(%) 雞糞 1:1:1:1 30 20 21 2:1:1:1 37 25 27 1:2:1:1 24 13 17 1:1:2:1 20 15 20 1:1:1:2 26 18 20 牛糞 1:1:1:1 20 16 33 2:1:1:1 27 20 39 1:2:1:1 17 12 30 1:1:2:1 13 11 25 1:1:1:2 17 14 30 香菇木屑 1:1:1:1 11 9 18 2:1:1:1 16 11 25 1:2:1:1 9 7 16 1:1:2:1 7 6 11 1:1:1:2 8 7 17 註釋*係指分解酵素、合成酵素、轉化酵素、聚合酵素的重量比例。 Table 3. Test results of total content of nitrogen, phosphorus and potassium in various ingredients Fertilizer raw materials Enzyme ratio* nitrogen phosphorus Potassium Percentage of total content (%) chicken manure 1:1:1:1 30 20 twenty one 2:1:1:1 37 25 27 1:2:1:1 twenty four 13 17 1:1:2:1 20 15 20 1:1:1:2 26 18 20 cow dung 1:1:1:1 20 16 33 2:1:1:1 27 20 39 1:2:1:1 17 12 30 1:1:2:1 13 11 25 1:1:1:2 17 14 30 Mushroom Sawdust 1:1:1:1 11 9 18 2:1:1:1 16 11 25 1:2:1:1 9 7 16 1:1:2:1 7 6 11 1:1:1:2 8 7 17 Note* refers to the weight ratio of catabolic enzymes, synthetic enzymes, inverting enzymes, and polymeric enzymes.

此結果顯示分解酵素、合成酵素、轉化酵素、聚合酵素以不同的比例混合,再與三種製肥原料作用,其所得之配料會以不同含量比率釋放氮、磷、鉀。結果顯示,酵素間混合比例為2:1:1:1時,可得到釋放養分氮、磷、鉀含量為最佳之配料。The results show that catalyzing enzymes, synthetic enzymes, inverting enzymes, and polymerizing enzymes are mixed in different proportions, and then react with the three fertilizer raw materials, and the resulting ingredients will release nitrogen, phosphorus, and potassium in different content ratios. The results show that when the mixing ratio of enzymes is 2:1:1:1, the best ingredients for releasing nutrients nitrogen, phosphorus and potassium can be obtained.

實施例二:各種配料於土壤中於一定時間內所釋放之養分含量之檢測Embodiment 2: Detection of nutrient content released by various ingredients in soil within a certain period of time

將3種製肥原料各秤10公斤,再於該些製肥原料中各自加入80公克的分解酵素、40公克合成酵素、40公克轉化酵素、40公克聚合酵素之酵素混合物(即酵素間混合比例為2:1:1:1),再依照實施例一之方法,即可製得三種配料。將該些配料各自添加10公斤土壤,再加入滅菌水至配料與土壤的混合物之含水分為50 %。將配料與土壤的混合物置放在25 ℃暗式孵育箱中360天,期間每30天分別取100公克土壤樣品進行檢測,檢測方法同實施例一。以雞糞、牛糞與香菇木屑所製得之配料,針對於不同時間點所釋放的養分種類與含量的檢測結果分別如表4、表5及表6所示。Weigh 3 kinds of fertilizer raw materials by 10 kg each, and then add 80 grams of decomposition enzymes, 40 grams of synthetic enzymes, 40 grams of invertase, and 40 grams of polymerase enzymes into these fertilizer raw materials. 2:1:1:1), and then according to the method of Example 1, three kinds of ingredients can be obtained. 10 kg of soil was added to each of these ingredients, and then sterilized water was added until the moisture content of the mixture of ingredients and soil was 50%. The mixture of ingredients and soil was placed in a dark incubator at 25° C. for 360 days, during which 100 grams of soil samples were taken every 30 days for detection, and the detection method was the same as that in Example 1. With the ingredients prepared from chicken manure, cow manure and shiitake mushroom sawdust, the detection results of the types and contents of nutrients released at different time points are shown in Table 4, Table 5 and Table 6, respectively.

表4:以雞糞製得之配料之養分釋放結果 天數 總累積含量的百分率(%) 0 37 25 27 30 45 41 40 60 57 51 52 90 69 58 63 120 74 63 69 150 78 69 72 180 81 74 77 210 85 76 80 240 87 80 84 270 90 83 87 300 93 85 91 330 95 87 94 360 97 90 96 Table 4: Nutrient release results of ingredients prepared from chicken manure days nitrogen phosphorus Potassium Percentage of total accumulated content (%) 0 37 25 27 30 45 41 40 60 57 51 52 90 69 58 63 120 74 63 69 150 78 69 72 180 81 74 77 210 85 76 80 240 87 80 84 270 90 83 87 300 93 85 91 330 95 87 94 360 97 90 96

表5:以牛糞製得之配料之養分釋放結果 天數 總累積含量的百分率(%) 0 27 20 39 30 38 30 42 60 43 36 46 90 50 43 51 120 57 49 56 150 61 52 61 180 68 59 65 210 73 65 71 240 78 73 75 270 85 80 83 300 89 84 87 330 92 89 91 360 96 92 95 Table 5: Nutrient release results for ingredients made from cow dung days nitrogen phosphorus Potassium Percentage of total accumulated content (%) 0 27 20 39 30 38 30 42 60 43 36 46 90 50 43 51 120 57 49 56 150 61 52 61 180 68 59 65 210 73 65 71 240 78 73 75 270 85 80 83 300 89 84 87 330 92 89 91 360 96 92 95

表6:以香菇木屑製得之配料之養分釋放結果 天數 總累積含量的百分率(%) 0 16 11 25 30 20 19 31 60 27 25 34 90 32 30 38 120 39 36 43 150 46 40 48 180 51 51 53 210 61 60 63 240 73 75 73 270 80 81 80 300 85 84 85 330 89 87 89 360 94 92 95 Table 6: Nutrient release results of ingredients prepared from shiitake mushroom sawdust days nitrogen phosphorus Potassium Percentage of total accumulated content (%) 0 16 11 25 30 20 19 31 60 27 25 34 90 32 30 38 120 39 36 43 150 46 40 48 180 51 51 53 210 61 60 63 240 73 75 73 270 80 81 80 300 85 84 85 330 89 87 89 360 94 92 95

此結果顯示以雞糞、牛糞、香菇木屑製得的配料於土壤孵育的360天期間,於各時間點所釋放出之養分含量並不相同。以雞糞製得之配料於第60天就能釋放氮、磷、鉀養分達到50%以上,這說明以雞糞製得之配料中的酵素分解製肥原料的速度快。以牛糞製得之配料則需90至120天,氮、磷、鉀養分釋放始能達到約50%。香菇木屑製得之配料則需約180天。This result shows that the nutrient content released at each time point is not the same during the 360-day incubation period of the ingredients prepared with chicken manure, cow manure, and shiitake mushroom sawdust. The ingredients made from chicken manure can release more than 50% of nitrogen, phosphorus and potassium nutrients on the 60th day, which shows that the enzymes in the ingredients made from chicken manure can decompose the fertilizer raw materials quickly. The ingredients made from cow dung take 90 to 120 days before the release of nitrogen, phosphorus and potassium nutrients can reach about 50%. It takes about 180 days to make the ingredients from shiitake sawdust.

實施例三:複數種複合肥料於土壤中於一定時間內所釋放之養分含量之檢測Example 3: Detection of Nutrient Content Released by Multiple Compound Fertilizers in Soil within a Certain Time

將實施例二所製得的三種配料以不同比例混合,以製得複數種類的複合肥料。以雞糞製得的配料、牛糞製得的配料、香菇木屑製得的配料之混合比例為1:1:1、2:1:1、1:2:1、1:1:1、1:1:2、3:1:1、4:1:1、5:1:1、2:1:0、2:0:1、2:2:0、0:1:1。接著,該些複合肥料各自添加10公斤土壤,再加入滅菌水至複合肥料與土壤的混合物之含水分為50 %。將複合肥料與土壤的混合物置放在25 ℃暗式孵育箱中360天,期間每30天分別取100公克土壤樣品進行檢測,檢測方法同實施例一,檢測結果分別如表7、表8及表9所示。The three ingredients prepared in Example 2 were mixed in different proportions to prepare multiple types of compound fertilizers. The mixing ratios of ingredients prepared from chicken manure, ingredients prepared from cow dung, and ingredients prepared from mushroom sawdust are 1:1:1, 2:1:1, 1:2:1, 1:1:1, 1:1: 1:2, 3:1:1, 4:1:1, 5:1:1, 2:1:0, 2:0:1, 2:2:0, 0:1:1. Next, each of these compound fertilizers was added with 10 kilograms of soil, and then sterilized water was added until the water content of the mixture of compound fertilizer and soil was 50%. The mixture of compound fertilizer and soil was placed in a dark incubator at 25 °C for 360 days, during which 100 grams of soil samples were taken every 30 days for detection. shown in Table 9.

表7 天數 雞糞:牛糞:香菇木屑 1: 1: 1 2: 1: 1 1: 2: 1 1: 1: 2 3: 1: 1 4: 1: 1 5: 1: 1 2: 1: 0 2: 0: 1 2: 2: 0 1: 2: 1 釋放總氮(N)養分總累積百分率(%) 0 26.7 29.3 26.8 24.0 30.8 31.8 32.6 33.7 30.0 32.0 21.5 30 34.3 37.0 35.3 30.8 38.6 39.7 40.4 42.7 36.7 41.5 29.0 60 42.3 46.0 42.5 38.5 48.2 49.7 50.7 52.3 47.0 50.0 35.0 90 50.3 55.0 50.3 45.8 57.8 59.7 61.0 62.7 56.7 59.5 41.0 120 56.7 61.0 56.8 52.3 63.6 65.3 66.6 68.3 62.3 65.5 48.0 150 61.7 65.8 61.5 57.8 68.2 69.8 71.0 72.3 67.3 69.5 53.5 180 66.7 70.3 67.0 62.8 72.4 73.8 74.9 76.7 71.0 74.5 59.5 210 73.0 76.0 73.0 70.0 77.8 79.0 79.9 81.0 77.0 79.0 67.0 240 79.3 81.3 79.0 77.8 82.4 83.2 83.7 84.0 82.3 82.5 75.5 270 85.0 86.3 85.0 83.8 87.0 87.5 87.9 88.3 86.7 87.5 82.5 300 89.0 90.0 89.0 88.0 90.6 91.0 91.3 91.7 90.3 91.0 87.0 330 92.0 92.8 92.0 91.3 93.2 93.5 93.7 94.0 93.0 93.5 90.5 360 95.7 96.0 95.8 95.3 96.2 96.3 96.4 96.7 96.0 96.5 95.0 Table 7 days Chicken Dung: Cow Dung: Mushroom Sawdust 1:1:1 2:1:1 1:2:1 1:1:2 3:1:1 4:1:1 5:1:1 2:1:0 2:0:1 2:2:0 1:2:1 Release of total nitrogen (N) nutrient total accumulation percentage (%) 0 26.7 29.3 26.8 24.0 30.8 31.8 32.6 33.7 30.0 32.0 21.5 30 34.3 37.0 35.3 30.8 38.6 39.7 40.4 42.7 36.7 41.5 29.0 60 42.3 46.0 42.5 38.5 48.2 49.7 50.7 52.3 47.0 50.0 35.0 90 50.3 55.0 50.3 45.8 57.8 59.7 61.0 62.7 56.7 59.5 41.0 120 56.7 61.0 56.8 52.3 63.6 65.3 66.6 68.3 62.3 65.5 48.0 150 61.7 65.8 61.5 57.8 68.2 69.8 71.0 72.3 67.3 69.5 53.5 180 66.7 70.3 67.0 62.8 72.4 73.8 74.9 76.7 71.0 74.5 59.5 210 73.0 76.0 73.0 70.0 77.8 79.0 79.9 81.0 77.0 79.0 67.0 240 79.3 81.3 79.0 77.8 82.4 83.2 83.7 84.0 82.3 82.5 75.5 270 85.0 86.3 85.0 83.8 87.0 87.5 87.9 88.3 86.7 87.5 82.5 300 89.0 90.0 89.0 88.0 90.6 91.0 91.3 91.7 90.3 91.0 87.0 330 92.0 92.8 92.0 91.3 93.2 93.5 93.7 94.0 93.0 93.5 90.5 360 95.7 96.0 95.8 95.3 96.2 96.3 96.4 96.7 96.0 96.5 95.0

表8   天數 雞糞:牛糞:香菇木屑 1: 1: 1 2: 1: 1 1: 2: 1 1: 1: 2 3: 1: 1 4: 1: 1 5: 1: 1 2: 1: 0 2: 0: 1 2: 2: 0 0: 1: 1   釋放磷(P)養分總累積百分率(%) 0 18.7 20.3 19.0 16.8 21.2 21.8 22.3 23.3 20.3 22.5 15.5 30 30.0 32.8 30.0 27.3 34.4 35.5 36.3 37.3 33.7 35.5 24.5 60 37.3 40.8 37.0 34.3 42.8 44.2 45.1 46.0 42.3 43.5 30.5 90 43.7 47.3 43.5 40.3 49.4 50.8 51.9 53.0 48.7 50.5 36.5 120 49.3 52.8 49.3 46.0 54.8 56.2 57.1 58.3 54.0 56.0 42.5 150 53.7 57.5 53.3 50.3 59.8 61.3 62.4 63.3 59.3 60.5 46.0 180 61.3 64.5 60.8 58.8 66.4 67.7 68.6 69.0 66.3 66.5 55.0 210 67.0 69.3 66.5 65.3 70.6 71.5 72.1 72.3 70.7 70.5 62.5 240 76.0 77.0 75.3 75.8 77.6 78.0 78.3 77.7 78.3 76.5 74.0 270 81.3 81.8 81.0 81.3 82.0 82.2 82.3 82.0 82.3 81.5 80.5 300 84.3 84.5 84.3 84.3 84.6 84.7 84.7 84.7 84.7 84.5 84.0 330 87.7 87.5 88.0 87.5 87.4 87.3 87.3 87.7 87.0 88.0 88.0 360 91.3 91.0 91.5 91.5 90.8 90.7 90.6 90.7 90.7 91.0 92.0 Table 8 days Chicken Dung: Cow Dung: Mushroom Sawdust 1:1:1 2:1:1 1:2:1 1:1:2 3:1:1 4:1:1 5:1:1 2:1:0 2:0:1 2:2:0 0:1:1 Released Phosphorus (P) Nutrient Total Accumulation Percentage (%) 0 18.7 20.3 19.0 16.8 21.2 21.8 22.3 23.3 20.3 22.5 15.5 30 30.0 32.8 30.0 27.3 34.4 35.5 36.3 37.3 33.7 35.5 24.5 60 37.3 40.8 37.0 34.3 42.8 44.2 45.1 46.0 42.3 43.5 30.5 90 43.7 47.3 43.5 40.3 49.4 50.8 51.9 53.0 48.7 50.5 36.5 120 49.3 52.8 49.3 46.0 54.8 56.2 57.1 58.3 54.0 56.0 42.5 150 53.7 57.5 53.3 50.3 59.8 61.3 62.4 63.3 59.3 60.5 46.0 180 61.3 64.5 60.8 58.8 66.4 67.7 68.6 69.0 66.3 66.5 55.0 210 67.0 69.3 66.5 65.3 70.6 71.5 72.1 72.3 70.7 70.5 62.5 240 76.0 77.0 75.3 75.8 77.6 78.0 78.3 77.7 78.3 76.5 74.0 270 81.3 81.8 81.0 81.3 82.0 82.2 82.3 82.0 82.3 81.5 80.5 300 84.3 84.5 84.3 84.3 84.6 84.7 84.7 84.7 84.7 84.5 84.0 330 87.7 87.5 88.0 87.5 87.4 87.3 87.3 87.7 87.0 88.0 88.0 360 91.3 91.0 91.5 91.5 90.8 90.7 90.6 90.7 90.7 91.0 92.0

表9   天數 雞糞:牛糞:香菇木屑 1: 1: 1 2: 1: 1 1: 2: 1 1: 1: 2 3: 1: 1 4: 1: 1 5: 1: 1 2: 1: 0 2: 0: 1 2: 2: 0 0: 1: 1   釋放鉀(K)養分總累積百分率(%) 0 30.3 29.5 32.5 29.0 29.0 28.7 28.4 31.0 26.3 33.0 32.0 30 37.7 38.3 38.8 36.0 38.6 38.8 39.0 40.7 37.0 41.0 36.5 60 44.0 46.0 44.5 41.5 47.2 48.0 48.6 50.0 46.0 49.0 40.0 90 50.7 53.8 50.8 47.5 55.6 56.8 57.7 59.0 54.7 57.0 44.5 120 56.0 59.3 56.0 52.8 61.2 62.5 63.4 64.7 60.3 62.5 49.5 150 60.3 63.3 60.5 57.3 65.0 66.2 67.0 68.3 64.0 66.5 54.5 180 65.0 68.0 65.0 62.0 69.8 71.0 71.9 73.0 69.0 71.0 59.0 210 71.3 73.5 71.3 69.3 74.8 75.7 76.3 77.0 74.3 75.5 67.0 240 77.3 79.0 76.8 76.3 80.0 80.7 81.1 81.0 80.3 79.5 74.0 270 83.3 84.3 83.3 82.5 84.8 85.2 85.4 85.7 84.7 85.0 81.5 300 87.7 88.5 87.5 87.0 89.0 89.3 89.6 89.7 89.0 89.0 86.0 330 91.3 92.0 91.3 90.8 92.4 92.7 92.9 93.0 92.3 92.5 90.0 360 95.3 95.5 95.3 95.3 95.6 95.7 95.7 95.7 95.7 95.5 95.0 Table 9 days Chicken Dung: Cow Dung: Mushroom Sawdust 1:1:1 2:1:1 1:2:1 1:1:2 3:1:1 4:1:1 5:1:1 2:1:0 2:0:1 2:2:0 0:1:1 Released potassium (K) nutrient total accumulation percentage (%) 0 30.3 29.5 32.5 29.0 29.0 28.7 28.4 31.0 26.3 33.0 32.0 30 37.7 38.3 38.8 36.0 38.6 38.8 39.0 40.7 37.0 41.0 36.5 60 44.0 46.0 44.5 41.5 47.2 48.0 48.6 50.0 46.0 49.0 40.0 90 50.7 53.8 50.8 47.5 55.6 56.8 57.7 59.0 54.7 57.0 44.5 120 56.0 59.3 56.0 52.8 61.2 62.5 63.4 64.7 60.3 62.5 49.5 150 60.3 63.3 60.5 57.3 65.0 66.2 67.0 68.3 64.0 66.5 54.5 180 65.0 68.0 65.0 62.0 69.8 71.0 71.9 73.0 69.0 71.0 59.0 210 71.3 73.5 71.3 69.3 74.8 75.7 76.3 77.0 74.3 75.5 67.0 240 77.3 79.0 76.8 76.3 80.0 80.7 81.1 81.0 80.3 79.5 74.0 270 83.3 84.3 83.3 82.5 84.8 85.2 85.4 85.7 84.7 85.0 81.5 300 87.7 88.5 87.5 87.0 89.0 89.3 89.6 89.7 89.0 89.0 86.0 330 91.3 92.0 91.3 90.8 92.4 92.7 92.9 93.0 92.3 92.5 90.0 360 95.3 95.5 95.3 95.3 95.6 95.7 95.7 95.7 95.7 95.5 95.0

上述結果顯示以5:1:1、2:1:0或2:2:0混合配料所製得的複合肥料,在第30天即釋放養分總氮達40 %以上。以2:1:0混合配料所製得的複合肥料,能最快提供磷。以2:2:0混合配料所製得的複合肥料,則是能最快提供鉀。以1:1:2或1:2:1混合配料所製得的複合肥料,釋放養分總氮、磷、鉀含量均較慢,釋放養分氮及鉀達50 %以上均需要90~120天 (表7及表9),釋放養分磷達50%以上均需要150天 (表8)。The above results show that the compound fertilizer prepared by mixing ingredients at 5:1:1, 2:1:0 or 2:2:0 can release more than 40% of total nitrogen in the 30th day. The compound fertilizer prepared by mixing ingredients in 2:1:0 can provide phosphorus the fastest. The compound fertilizer prepared by mixing ingredients at 2:2:0 can provide potassium the fastest. The compound fertilizer prepared by mixing the ingredients at 1:1:2 or 1:2:1 releases the nutrient content of total nitrogen, phosphorus and potassium slowly, and it takes 90 to 120 days to release the nutrient nitrogen and potassium above 50% ( Table 7 and Table 9), it takes 150 days to release more than 50% of nutrient phosphorus (Table 8).

實施例四:依作物需求挑選標的複合肥料Embodiment 4: Select the target compound fertilizer according to the needs of crops

複數種複合肥料的養分釋放時間與含量的檢測結果之表建立後,即能根據標的作物生長期中不同階段所需要的養分量,來從此檢測結果之表中挑選出標的複合肥料。After the table of the test results of nutrient release time and content of multiple compound fertilizers is established, the target compound fertilizer can be selected from the table of test results according to the amount of nutrients required in different stages of the target crop's growth period.

作物生長期需求之複合肥料的一般原則:葉菜類全期或果樹類幼果期需要氮:磷:鉀釋放量為高:中:低。例如:複合肥料於30天氮:磷:鉀釋放量分別為至少8%(例如:8%~15%):6%~8%:2%~3%。果菜類中期或果樹類中果期需要氮:磷:鉀釋放量為低:高:高。例如:複合肥料於30天氮:磷:鉀釋放量分別為2%~3%:至少8%(例如:8%~15%):至少8%(例如:8%~15%)。專業上仍需依據測定該土壤性質與養分含量及植體分析之結果為精準之施用肥料量。The general principle of compound fertilizers required in the growing season of crops: the whole period of leafy vegetables or the young fruit period of fruit trees requires nitrogen: phosphorus: potassium release amount is high: medium: low. For example, the nitrogen: phosphorus: potassium release of compound fertilizers in 30 days is at least 8% (for example: 8% ~ 15%): 6% ~ 8%: 2% ~ 3%. The nitrogen: phosphorus: potassium release is low: high: high in the middle fruiting stage of fruit vegetables or fruit trees. For example: Nitrogen: Phosphorus: Potassium release of compound fertilizers in 30 days is 2% to 3%: at least 8% (for example: 8% to 15%): at least 8% (for example: 8% to 15%). Professionally, it is still necessary to determine the exact amount of fertilizer applied based on the results of soil properties and nutrient content and plant analysis.

舉例來說,蔬菜類幼苗期需要大量氮肥,就適合施予以5:1:1、2:1:0或2:2:0混合配料所製得的複合肥料。花草菜類幼苗期需要較大量磷肥,就適合施予以2:1:0混合配料所製得的複合肥料。根莖菜類需要較大量鉀肥,就適合施予以2:2:0混合配料所製得的複合肥料。生長期較長的作物或多年生作物,例如果樹、蘆筍,就適合施予以1:1:2或1:2:1混合配料所製得的複合肥料。For example, vegetable seedlings require a lot of nitrogen fertilizers, so it is suitable to apply compound fertilizers prepared by mixing ingredients in 5:1:1, 2:1:0 or 2:2:0. The seedling stage of flowers and vegetables requires a large amount of phosphate fertilizer, so it is suitable to apply the compound fertilizer prepared by mixing the ingredients in 2:1:0. Root vegetables require a large amount of potassium fertilizer, so it is suitable to apply a compound fertilizer prepared by mixing ingredients in a 2:2:0 ratio. For crops with long growth period or perennial crops, such as fruit trees and asparagus, it is suitable to apply compound fertilizer prepared by mixing ingredients in 1:1:2 or 1:2:1.

以此類推,只要依據作物需要養分總氮、磷、鉀含量的時期及比例,即可查找預先建置完成之揭露有土壤樣品養分之表(如表7、表8、表9),自表中選擇最適合的組合,即可達成提供快速生產精準型釋放長效有機質肥料的目的。甚至以本揭露的施肥方法,還能因應不同種類的製肥原料、酵素,找出酵素混合的較佳比例,再以此方法建置複合肥料的養分釋放表,就同樣能針對標的作物精準地施予肥料。By analogy, as long as the period and ratio of total nitrogen, phosphorus, and potassium content of nutrients required by crops, the pre-built tables that disclose soil sample nutrients (such as Table 7, Table 8, and Table 9) can be found. By choosing the most suitable combination from among them, the purpose of providing rapid production and precise release of long-acting organic matter fertilizer can be achieved. Even with the fertilization method disclosed in the present disclosure, it is possible to find out the optimal ratio of enzyme mixing in response to different types of fertilizer raw materials and enzymes, and then build a nutrient release table of compound fertilizers in this way, which can also accurately target crops. Apply fertilizer.

本揭露之施肥方法藉由檢測以不同比例混合配料所完成的複數種複合肥料於土壤中所釋放出養分種類及養分含量,可明確得知該些複合肥料於單位時間內所釋放出之養分含量,並依據目標作物之施肥需求及該檢測結果,選擇目標作物所適合的複合肥料進行施肥,可使本揭露之施肥方法較為傳統方法更為精準地調整肥料中養分的釋放量與釋放時間。亦因如此,以本揭露之施肥方法進行施肥,還可減少「追肥」之情形發生,進而可減少人力及時間成本。The fertilization method of the present disclosure can clearly know the nutrient content released by the compound fertilizers in a unit time by detecting the types and contents of nutrients released in the soil by a plurality of compound fertilizers prepared by mixing ingredients in different proportions. , and according to the fertilization requirements of the target crops and the detection results, the compound fertilizer suitable for the target crops is selected for fertilization, so that the fertilization method disclosed in the present disclosure can more accurately adjust the release amount and release time of nutrients in the fertilizer compared with the traditional methods. Because of this, fertilizing with the fertilizing method of the present disclosure can also reduce the occurrence of "top dressing", thereby reducing labor and time costs.

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Claims (11)

一種施肥方法,其包含下列步驟: 複數種類的製肥原料分別施予一酵素,以製得複數個配料; 該些配料以不同比例混合,以製得複數種類的複合肥料; 將該些複合肥料各自加入土壤,以得複數個土壤樣品; 檢測該些土壤樣品,以得一檢測結果,其揭露該些土壤樣品之養分含量; 根據一作物的施肥需求比對該些養分含量,以從複數種類的複合肥料中選擇一標的複合肥料;及 對該作物施予該標的複合肥料。 A fertilization method comprising the following steps: A plurality of types of fertilizer raw materials are respectively applied with an enzyme to obtain a plurality of ingredients; The ingredients are mixed in different proportions to obtain multiple types of compound fertilizers; adding these compound fertilizers to soil respectively to obtain a plurality of soil samples; testing the soil samples to obtain a testing result that reveals the nutrient content of the soil samples; Comparing these nutrient contents according to the fertilization requirements of a crop to select a target compound fertilizer from a plurality of types of compound fertilizers; and Apply the target compound fertilizer to the crop. 如請求項1所述之施肥方法,其中該製肥原料為生物質。The fertilization method according to claim 1, wherein the fertilization raw material is biomass. 如請求項1所述之施肥方法,其中該生物質為雞糞、牛糞或真菌類培養用木屑。The fertilization method according to claim 1, wherein the biomass is chicken manure, cow manure or wood chips for fungal culture. 如請求項1所述之施肥方法,其中該酵素為分解酵素(hydrolases)、合成酵素(synthetases)、轉化酵素(transferases)、聚合酵素(polymerases)或前述酵素兩種以上之組合。The fertilizing method of claim 1, wherein the enzymes are hydrolases, synthetases, transferases, polymerases, or a combination of two or more of the foregoing enzymes. 如請求項4所述之施肥方法,其中該酵素為分解酵素、合成酵素、轉化酵素及聚合酵素(polymerases)之混合物,其混合比例以重量比計,為1~5:1~5:1~5:1~5。The fertilization method according to claim 4, wherein the enzyme is a mixture of decomposing enzymes, synthetic enzymes, inverting enzymes and polymerases, and the mixing ratio is 1-5:1-5:1- 5:1 to 5. 如請求項1所述之施肥方法,其中該些複合肥料係以雞糞製得之配料與以牛糞製得之配料,各自以介於1:2~3:1之重量比混合而成。The fertilization method according to claim 1, wherein the compound fertilizers are prepared by mixing chicken manure ingredients and cow manure ingredients in a weight ratio of 1:2 to 3:1. 如請求項1所述之施肥方法,其中該些複合肥料係以雞糞製得之配料與以真菌類培養用木屑製得之配料,各自以介於1:2~3:1之重量比混合而成。The fertilization method according to claim 1, wherein the compound fertilizers are the ingredients prepared from chicken manure and the ingredients prepared from wood chips for fungal culture, and are mixed in a weight ratio of 1:2 to 3:1. made. 如請求項1所述之施肥方法,其中該些複合肥料係以牛糞製得之配料與以真菌類培養用木屑製得之配料,各自以介於1:2~3:1之重量比混合而成。The fertilization method as claimed in claim 1, wherein the compound fertilizers are prepared from cow dung ingredients and fungi cultured wood chips, each of which is mixed in a weight ratio of 1:2 to 3:1. to make. 如請求項1所述之施肥方法,其中該些複合肥料係以雞糞製得之配料、以牛糞製得之配料與以真菌類培養用木屑製得之配料,各自以介於1~6:1~3:1~3之重量比混合而成。The fertilization method as claimed in claim 1, wherein the compound fertilizers are ingredients prepared from chicken manure, ingredients prepared from cow dung, and ingredients prepared from fungal culture wood chips, each of which is between 1 to 6: 1~3: The weight ratio of 1~3 is mixed. 如請求項1所述之施肥方法,其中該養分為氮、磷、鉀或前述養分兩種以上之組合。The fertilization method according to claim 1, wherein the nutrient is nitrogen, phosphorus, potassium, or a combination of two or more of the foregoing nutrients. 如請求項1所述之施肥方法,其中該作物為果樹類或蔬菜類。The fertilization method according to claim 1, wherein the crops are fruit trees or vegetables.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120110688A1 (en) * 2010-09-08 2012-05-03 Dow Agrosciences Llc Aad-12 event 1606 and related transgenic soybean lines
CN103342615A (en) * 2013-07-25 2013-10-09 瑞昊(北京)环境工程有限公司 Phosphorus and potassium decomposing biological organic fertilizer and preparation method thereof
TW201438581A (en) * 2012-11-29 2014-10-16 Bayer Cropscience Lp Methods of controlling fungal pathogens using polyene fungicides
CN110590457A (en) * 2019-10-16 2019-12-20 黄健 Biological organic bacterial fertilizer for crops and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120110688A1 (en) * 2010-09-08 2012-05-03 Dow Agrosciences Llc Aad-12 event 1606 and related transgenic soybean lines
TW201438581A (en) * 2012-11-29 2014-10-16 Bayer Cropscience Lp Methods of controlling fungal pathogens using polyene fungicides
CN103342615A (en) * 2013-07-25 2013-10-09 瑞昊(北京)环境工程有限公司 Phosphorus and potassium decomposing biological organic fertilizer and preparation method thereof
CN110590457A (en) * 2019-10-16 2019-12-20 黄健 Biological organic bacterial fertilizer for crops and preparation method thereof

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