JP6746020B1 - Compost fermentation accelerator, compost manufacturing method, compost fermentation method, composting material, compost fermentation product and culture soil - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a compost fermentation accelerator containing euglena, a compost manufacturing method using euglena, a compost fermentation method, a composting material, a compost fermented product and a culture soil. SOLUTION: A compost fermentation accelerator containing euglena, a compost manufacturing method in which euglena is added to a compost raw material for fermentation, a compost fermentation method in which euglena is added to a compost raw material for fermentation, a compost raw material and euglena are included It is a composting material, a compost raw material, and a compost fermentation product of Euglena, and a culture soil containing a compost raw material and a compost fermentation product of Euglena. [Selection diagram] Fig. 3

Description

The present invention relates to a compost fermentation accelerator, a compost manufacturing method, a compost fermentation method, a composting material, and a compost fermentation product, and a Euglena-containing compost fermentation accelerator, a compost manufacturing method, a compost fermentation method, a composting material, a compost fermentation product. And the culture soil.

Euglena (genus name: Euglena, Japanese name: Euglena) is attracting attention as a biological resource that is expected to be used as food, feed, fuel, and the like. Euglena has 59 kinds of nutrients corresponding to most of the nutrients necessary for humans to survive, such as vitamins, minerals, amino acids, and unsaturated fatty acids, and as a supplement to balance the intake of various kinds of nutrients. It has been proposed that it can be used or used as a food source in poor areas where the necessary nutrients cannot be obtained.

Euglena is a primary producer of the food chain and is preyed on by predators, and the culture conditions such as light, temperature conditions and agitation speed are more difficult than other microorganisms. However, in recent years, the inventors of the present invention have earnestly studied to establish a large-scale culturing technique, and have opened up a large-scale supply of Euglena-derived substances such as Euglena and paramylon extracted from Euglena.

Patent Document 1 describes an enzyme production promoter of koji mold, which contains a Euglena alga as an active ingredient and is used for promoting enzyme production of koji mold.

Japanese Patent No. 6251436

Although Patent Document 1 describes that Euglena has a fermentation promoting action on Aspergillus oryzae, its action on bacteria other than Aspergillus or application to fields other than foods and cosmetics has not been studied, and Euglena's food , The use in fields other than feed, cosmetics and biofuels was desired.

The present invention has been made in view of the above problems, and an object of the present invention is to provide a compost fermentation accelerator containing euglena, a compost manufacturing method using euglena, a compost fermentation method, a composting material, and a compost fermentation product. And to provide a culture soil.

As a result of intensive studies, the present inventors have found that Euglena has a fermentation promoting action on compost raw materials. More specifically, it has been clarified that Euglena functions as a compost fermentation accelerator that promotes fermentation in compost production, and that the obtained compost exerts an excellent fertilizing effect, and the present invention has been completed.

Therefore, according to the present invention, the above problem can be solved by a compost fermentation accelerator containing euglena which has been subjected to at least one of a drying treatment and a mechanical treatment .
Thus, by using Euglena for the fermentation of the compost raw material, the fermentation of the compost raw material can be promoted.

At this time, the Euglena is preferably used in an amount of 1 part by weight or more and less than 100 parts by weight with respect to 100 parts by weight of livestock excrement selected from cow dung, pig dung, chicken dung, horse dung, and a mixture thereof.

Further, according to the present invention, the above-mentioned problems can be solved by a method for producing compost, which comprises adding Euglena that has been subjected to at least one of a drying treatment and a mechanical treatment to a compost raw material and performing fermentation.

Further, according to the present invention, the above-mentioned problem is solved by a compost fermentation method characterized by adding Euglena which has been subjected to at least one of a drying treatment and a mechanical treatment to a compost raw material to perform fermentation.

Further, according to the present invention, the above-mentioned problems can be solved by a composting material containing a compost raw material and Euglena which has been subjected to at least one of a drying treatment and a mechanical treatment .

Further, according to the present invention, the above-mentioned problems can be solved by a compost fermentation product of a compost raw material and Euglena which has been subjected to at least one of a drying treatment and a mechanical treatment .

Furthermore, the object is achieved according to the present invention, a compost material, drying and at least one of processing mechanical processing is solved by culture soil containing compost fermentation products of Euglena was made.

According to the present invention, there are provided a compost fermentation accelerator containing euglena, which is a new utilization method of euglena, a compost manufacturing method that effectively utilizes euglena, a compost fermentation method, a composting material, a compost fermented product, and a culture soil. You can

It is a graph which shows the result of the temperature measurement in the container of each test section in the test 1. It is a graph which shows pH of the compost supernatant obtained in test 2. It is a figure which shows the quantity of each component in the compost fermentation process measured in test 2. It is a graph which shows the germination rate of each test section measured in the test 3. It is a graph which shows the seedling root length of each test plot measured in Test 3. It is a graph which shows the above-ground part length of the young plant of each test area measured in the test 3. It is a graph which shows the yield of the komatsuna of each test section measured in the test 4. It is a graph which shows the result of the temperature measurement in the container of each test area in the test 5. It is a graph which shows the germination rate of each test section measured in the test 5. It is a graph which shows the seedling root length of each test plot measured in Test 5. It is a graph which shows the seedling aboveground length of each test plot measured in test 5. It is a graph which shows the quantity of each component in the compost fermentation process measured in test 5.

Embodiments of the present invention will be described below with reference to FIGS. 1 to 12.
The present embodiment relates to a compost fermentation accelerator containing euglena as an active ingredient, a compost production method using euglena, a compost fermentation method, a composting material, and a compost fermentation product.

<Euglena>
In an embodiment, “euglena” includes a taxonomically classified microorganism belonging to the genus Euglena, a variant thereof, a variant thereof, and a closely related species of the family Euglenae. Here, the genus Euglena is a group of organisms that belong to the group of eukaryotes that belong to Excabata, Euglenozoa phylum, Euglena alga, Euglena, and Euglena.

Specific examples of species included in the genus Euglena include Euglena chadefaudii, Euglena deses, Euglena gracilis, Euglena granulata, Euglena mutabilis, Euglena proxima, Euglena spirogyra, Euglena viridis. As Euglena, Euglena gracilis (E. gracilis), in particular, Euglena gracilis Z strain can be used, but in addition, a mutant strain of Euglena gracilis Z strain SM-ZK strain (Chloroplast deficient strain), variant E. gracilis var. bacillaris, gene mutants such as chloroplast mutants of these species, and other Euglena such as Astasia longa.

The genus Euglena is widely distributed in fresh water such as ponds and swamps, and may be used separately from these, or any Euglena genus that has already been isolated may be used. Euglena includes all mutants thereof. Further, these mutant strains also include those obtained by genetic methods such as recombination, transduction and transformation.

(Euglena algae)
In the present embodiment, it is possible to use Euglena algal cells as Euglena. As Euglena algal cells, live Euglena cells separated by centrifugation, filtration, sedimentation, or the like can be used as they are. The Euglena viable cells can be used as they are after being harvested from the culture tank, but they are preferably washed with water or physiological saline. Further, the Euglena algal cells may be used in the state of a dispersion liquid in which a liquid such as water is dispersed. In the present embodiment, it is preferable to use, as the Euglena algal cells, the dry algal cells of Euglena (euglena powder) obtained by freeze-drying or spray-drying live Euglena cells.

Further, a mechanically treated product of an alga body obtained by subjecting living Euglena cells to ultrasonic treatment or mechanical treatment such as homogenization may be used as a Euglena alga body. Further, a dried mechanically processed product obtained by subjecting the mechanically processed product to a drying treatment may be used as the Euglena algal cells.

<Compost fermentation accelerator>
The compost fermentation accelerator of the present embodiment contains Euglena as an active ingredient and has an action of promoting fermentation of the compost raw material.

As used herein, the term "compost" refers to an organic material that has been matured by depositing or stirring livestock excrement, food waste, food industry residues, and other organic substances in a controlled manner. The compost is processed to be microbiologically stabilized by heat generated during the ripening process, effective for plant growth and soil improvement, and easy to handle.

In addition, “composting” means that organic matter is deposited, agitated and aerated to make it aerobic, and the organic matter in the raw material is decomposed by microorganisms, and the heat generated by this decomposition evaporates water, causing pathogenic bacteria and parasites. Eggs and weed seeds are killed or inactivated to be safe, hygienic, and stable.

The decomposition process of organic matter is divided into primary fermentation in which easily decomposable organic matter is mainly decomposed and secondary fermentation in which decomposition and humification of degradable organic matter progress. Here, “accelerating the fermentation of the compost raw material” means accelerating the decomposition process of organic matter in composting.

As shown in Examples described later, when using livestock excrement as a material to be fermented, compost is applied to 100 parts by weight of livestock excrement selected from cow dung, pig dung, chicken dung, horse dung, and a mixture thereof. Euglena, which is an active ingredient of the fermentation accelerator, is preferably used in an amount of 1 part by weight or more and less than 100 parts by weight.

<Composting materials>
In the present embodiment, fermentation is performed by using a composting material containing a compost raw material and Euglena as a raw material. The compost raw material is a raw material for composting that includes a raw material to be fermented and an auxiliary material.

(Fermented material)
The type of raw material to be fermented (main material) when producing compost using the fermentation accelerator of the present invention is not particularly limited, and any organic fertilizer raw material (organic waste) that can be composted can be used. is there. Examples of the material to be fermented include livestock excrement, food residue, activated sludge, wood residue, fishery waste, and the like. Among them, the fermentation accelerator of the present embodiment is effective for composting organic waste having a high water content such as livestock excrement, food residue, and activated sludge.

Examples of livestock excreta include cow dung, pig dung, chicken dung, horse dung, and mixtures thereof. Examples of food residues include leftover food, garbage, vegetable waste, okara, coffee grounds, tea grounds, fruit dregs, beer dregs, shochu dregs, canned processed dregs, food processed sludge, and mixtures thereof. Examples of the fishery wastes include fish roofs, fish cakes, shellfish cakes, seaweed residues, and mixtures thereof.

(Auxiliary materials)
The auxiliary material is a material for adjusting the properties of the material to be fermented. Examples of the auxiliary material include organic materials (coarse organic materials such as rice husks, rice straw, straw, sawdust, and bark) and inorganic material materials (such as zeolite, vermiculite, and perlite).

(Fermentative bacteria)
Normally, for livestock excrement, food waste such as garbage, and organic waste such as sludge, fermentation conditions such as aeration can be controlled by adjusting the fermentation conditions when the fermentation equipment is put in without adding fermenting bacteria. It is known that good aerobic fermentation is carried out if carried out, but the compost raw material may optionally additionally contain a fermenting bacterium as an inoculum. Examples of fermenting bacteria include, but are not limited to, bacteria such as Bacillus microorganisms, actinomycetes, filamentous fungi, nitrite bacteria, nitric acid bacteria, and cellulolytic bacteria.

(C/N ratio)
The C/N ratio in the compost raw material is adjusted to 10 or more and 30 or less, preferably 15 or more and 25 or less, more preferably 18 or more and 22 or less, and particularly preferably 19 or more and 21 or less (about 20). Here, the C/N ratio is the ratio of the amount of carbon (C) and the amount of nitrogen (N) contained in the compost raw material.

<Compost manufacturing method (compost fermentation method)>
The compost manufacturing method (compost fermentation method) of the present embodiment is characterized in that Euglena is added to a compost raw material to perform fermentation. Specifically, the compost manufacturing method (compost fermentation method) of the present embodiment includes a pretreatment step (step S1) of mixing Euglena and a compost raw material to prepare a composting material, and fermentation for fermenting the composting material. The process (step S2) is performed.

(Pretreatment process)
In the pretreatment process, Euglena and compost raw materials are mixed to prepare a composting material (step S1). At this time, the air permeability of the composting material is improved and the water content and pH are adjusted. Generally, a facility for receiving and supplying compost raw materials and a pretreatment facility are provided. The receiving and supplying facility is a facility that temporarily stores the compost raw material and the auxiliary materials to be added and stably supplies it to the pretreatment facility. The pretreatment facility is a facility that improves the properties of the raw material and facilitates the activation of microorganisms in the fermentation process, and adjusts the raw material to conditions that promote good fermentation.The raw material and the returned compost from the secondary fermentation facility are used. Are mixed, rice husks, sawdust and other auxiliary materials are added, and the auxiliary materials are crushed and dried.

(Fermentation process)
In the fermentation step, the composting material containing the Euglena and the compost raw material adjusted in the pretreatment step is fermented under predetermined fermentation conditions (step S2). The fermentation process is a basic process for composting, and is also an important process for determining the quality of manufactured compost. The fermentation process is generally divided into two stages of primary fermentation and secondary fermentation, but it cannot be said that there is a clear division. In the fermentation process, in order to achieve uniform fermentation of the entire fermentation equipment input, the input is turned over, moved, and aerated.

In the fermentation process, fermentation of the composting material with inoculum is performed under predetermined fermentation conditions in which conditions such as fermentation temperature, water evaporation amount, pH, period (fermentation days), cut back frequency, and aeration amount are appropriately selected. Although various fermentation conditions may be constant throughout the period, various fermentation conditions may be changed according to the fermentation period in order to promote fermentation.

The fermenting equipment used in the compost manufacturing method (compost fermenting method) is not particularly limited. For example, stacking type (box type, ridge groove type, stationary stacking type, container type) equipment, and mechanical turning type (Horizontal plane type, horizontal circular type, horizontal elliptical type, vertical single-stage type, vertical multi-stage type, rotary type) can be used.

<Fermented compost products>
The compost fermentation product of the present embodiment is a compost raw material and a compost fermentation product of Euglena, and the compost manufacturing method of the present embodiment (compost fermentation method) using the compost raw material of the present embodiment and a composting material containing Euglena as a raw material. It is obtained by fermentation.

Here, the compost fermented product includes the fermented product itself obtained by fermenting the composting material, but other than that, obtained by appropriately treating the fermented product, powder, dried product, extracted dried product, etc. Also included are processed products of fermentation products such as solids, extracts, dispersions, juices, liquids such as filtrates.

As shown in the following examples, when using the compost fermentation product of the present embodiment as compost,
By adding Euglena as a compost fermentation accelerator, nitrate nitrogen and water-soluble phosphoric acid are increased, and a good germination rate and an excellent fertilizing effect are exhibited.

<Cultivated soil>
When used as a compost, the fermented compost product of the present embodiment exhibits a good germination rate and an excellent fertilizing effect, and thus can be suitably used as a compost in culture soil. Here, the "cultivation soil" means soil mixed with soil or fertilizer, and is a material used as a base for growing plants instead of soil.

The culture soil may be packed in a container for plant cultivation and used by planting seeds or plant seedlings, or it may be used as a soil for existing land for improvement of fields or cultivation of new farmland. It may be used as a material.

Hereinafter, the present invention will be specifically described based on specific examples, but the present invention is not limited thereto.

Since Euglena has no cell wall, it is easily decomposed by soil bacteria, and since it contains a wide range of amino acids necessary for soil bacteria to ferment, it can be expected to promote fermentation. In addition, it is expected that the presence pattern of soil bacterial flora will change accordingly, so it is expected to have the characteristics of compost different from conventional ones.

Therefore, in the following examples, it was examined whether the fermentation is promoted by applying the Euglena powder when producing compost. Moreover, the difference when compared with the group to which the Euglena powder was not added was verified.

(Raw material for compost)
The following materials were used as compost raw materials. Table 1 shows information on each material, and Table 2 shows the amount of material required for composting. Dried chicken manure pellets were the main material and were used as a nitrogen source. Rice husk is a material that is hardly fermented by itself, used for ensuring air permeability and as a carbon source. Cow dung compost was used as an inoculum.

(Fermentation promoting material)
As the fermentation promoting material, Euglena powder and a commercially available fermentation accelerator (waste bacterial bed for compost) were used.

(Outline of test area)
Regarding the test section, 6 test sections (0.01 kg/0.1 kg/0.5 kg/1 kg/1.5 kg/2 kg) in which Euglena powder was used as a fermentation accelerating material and additive-free and fermentation accelerating agent (compost waste bacterial bed) Two test plots using 0.02 kg) were made into a total of 8 test plots (using an 80 L container).

The sample label of each test plot was as follows.
・Sample 1: No additive ・Sample 2: 20 g of waste bacterial bed for compost
・Sample 3: Euglena 10g
・Sample 4: Euglena 100g
・Sample 5: Euglena 500g
・Sample 6: Euglena 1kg
・Sample 7: Euglena 1.5kg
・Sample 8: Euglena 2kg

(Measurement item)
In the test, the following items were measured. The outside air temperature was regularly measured with a thermometer. Geothermal was measured daily at a fixed time. In the toxicity test, the germination rate of Komatsuna seeds was measured, and in the fertilizer component analysis, a PAC test was performed.

<Test 1: Transition of fermentation heat in each test area>
FIG. 1 shows the results of the temperature measurement inside the container of each test section. The graph of FIG. 1 shows the transition of the heat of fermentation, and the arrow indicates the timing at which the cutback was performed. Despite the small scale of 80 L, in the test section (Sample 5) containing 500 g of Euglena powder, the fermentation heat of 60° C. or higher was recorded on the day after the preparation. In addition, in the test section of Euglena 10 g (Sample 3) and the test section of 100 g (Sample 4), the temperature in the container was higher than that in the non-addition section (Sample 1) and the compost waste bacterial bed addition section (Sample 2). Was 6-8°C higher.

From the results of Test 1, it was suggested that the primary fermentation was promoted depending on the amount of Euglena added, and the organic matter in the compost raw material was mineralized by the action of microorganisms. It was also shown that this phenomenon reached a ceiling with the addition of 1.5 kg or more.

<Test 2: Chemical properties>
The pH of the obtained compost supernatant is shown in FIG. The pH increased with time in all the test plots. It was a reasonable result considering that ammonia is generated in the fermentation process and is biased toward alkalinity. In any test category, the pH after one month was in the neutral range of 7 to 8 and it was suggested that there is no problem in using it as compost.

The morphological change of nitrogen in the fermentation process is generally considered to change as ammonia nitrogen (NH ₄ )→nitrite nitrogen (NO ₂ )→nitrate nitrogen (NO ₃ ). Nitrogen that has been oxidized to nitrate nitrogen is absorbed from plant roots and assimilated. That is, it is considered that the fertilizing effect is high when the nitrate nitrogen (NO ₃ ) is contained. In addition, water-soluble phosphoric acid (PO ₄ ) is generated in parallel in the above process, which can be regarded as a scale for mineralizing compost. Since water-soluble phosphoric acid is one of the fertilizer components used as a fast-acting fertilizer and is involved in fertilization effect, the production of water-soluble phosphoric acid is also important in terms of composting and fertilization. FIG. 3 shows the results of measuring the amounts of each component in the compost fermentation process.

When the Euglena powder was added, the amounts of nitrate nitrogen (NO ₃ ) and water-soluble phosphoric acid (PO ₄ ) generated tended to increase as compared with the control group. From this result, it was suggested that the addition of Euglena activated the fermentation of microorganisms and promoted the decomposition of organic matter to inorganic matter.

<Test 3: Young plant toxicity test-germination rate>
It is known that when immature compost is applied, plant roots are damaged by the phenolic acids and organic acids contained therein, and growth and germination are suppressed. Therefore, a seedling test was conducted for the purpose of measuring the maturity of compost.

(experimental method)
A 3 g sample was placed in a Falcon tube, and 30 mL of ion-exchanged water was added thereto. Extraction was performed at 60° C. for 3 hours. At that time, the mixture was vortexed every 30 minutes. The supernatant was filtered to obtain a compost supernatant. 50 Komatsuna seeds were arranged in a petri dish, and 10 mL of the compost supernatant was gently added thereto. Tap water was used as the target area. It was left at 26° C. for 4 days. At the time of measuring the root length and the above-ground length, 10 plants were taken and measured (N=10).

The results of the germination rate measurement are shown in FIG. Since poultry manure is easily degradable, all the test plots showed germination rates almost equal to tap water in the samples one month after preparation. However, in Sample 1 and Sample 2, the germination rate was about 80% 2 weeks after the preparation, but in the Euglena-added section (Samples 3 to 6), the germination rate was almost the same as tap water, It was suggested that the germination inhibition observed in immature compost was eliminated. That is, it was suggested that the toxicity was reduced in the Euglena-added group two weeks after the preparation.

Figure 5 shows the results of measuring the seedling root length. Two weeks after the preparation, root growth tended to be inhibited in the non-addition group (Sample 1) and the compost waste bacterial bed addition group (Sample 2). In the Euglena-added plot, root growth was almost the same as in the control plot.

FIG. 6 shows the measurement results of the aboveground length of seedlings. Compared to tap water, which is the target area, and Sample 1 and Sample 2, the growth of the aboveground chief was predominantly promoted in Samples 3 to 6.

<Summary of tests 1 to 3>
In Tests 1 to 3, for the purpose of verifying the effect of applying Euglena powder during compost production, a mixture of chicken droppings and rice husks was used as a compost raw material, and changes in fermentation heat with time, component analysis, and seedling test were conducted. It was When the above test results were combined, it was found that when Euglena was added in an amount of 50 parts by weight or more to 1 kg (100 parts by weight) of chicken manure as the main material, a rapid primary fermentation heat was generated (FIG. 1). Moreover, when 1 part by weight of Euglena was added to 1 kg of chicken manure (100 parts by weight), nitrate nitrogen and water-soluble phosphoric acid increased, suggesting acceleration of fermentation by activation of microorganisms (FIG. 3).

Furthermore, in the seedling test, the toxicity as immature compost was reduced from 2 weeks after the preparation, and not only the improvement of the germination rate was observed but also the fertilizing effect was observed (FIGS. 4 to 6).

<Test 4: Verification of effects on crop cultivation>
In the above test, it was suggested that a mixture of chicken manure and rice husks was used as a composting material, euglena powder was added to promote ripening, and that the Komatsuna seedling test showed fertilizing effect as compared with the target group. .. Next, the effect of actually applying the obtained compost on the growth of komatsuna was examined.

(Outline of the test)
In order to verify the effect of compost obtained by adding Euglena on crop cultivation, a cultivation test was conducted using Komatsuna seeds, which has a short period from sowing to harvest and is also used for registering fertilizers.

Komatsuna was grown from Komatsuna seeds using a home-use planter. The test plots at that time were as follows. The number of repetitions in each test group was set to 2 for the purpose of verifying reproducibility. When arranging the planters, a completely random arrangement using the statistical analysis language R was used to prevent the influence of the place.

・Test area 1: No fertilization ・Test area 2: Chicken manure and rice husk compost (no addition, commercial compost)
・Test area 3: Chicken manure and rice husk compost + waste compost bed for compost ・Test area 4: Chicken manure and husk compost + Euglena powder 10g (0.0125%)
-Test area 5: Chicken manure and rice husk compost + Euglena powder 100g (0.125%)
・Test area 6: Chicken manure rice husk compost + Euglena powder 500g (0.625%)
・Test area 7: Chicken manure and rice husk compost + Euglena powder 2 kg (2.5%)
・Test area 8: Chemical fertilizer (N:P:K=8:8:8) Fertilization (specified by the manufacturer)

Cultivation period: September 5th to October 4th (one month)
Soil used: Kuroboku soil, approx. 20 L/planter Fertilization rate: 4 L/planter

FIG. 7 shows the yield of komatsuna in each test section. In the fermentation-promoting composting area in which Euglena was added to the compost raw material volume at 0.125% (test area 5), 0.625% (test area 6), and 2.5% (test area 7), chicken manure and rice husk compost (no A yield increase of about 41% was observed for the addition and test plots 2), and a yield increase of about 23% for the compost waste bacterial bed addition plot (test plot 3). From this result, it was shown that the addition of Euglena powder to the compost raw material not only promotes compost fermentation, but also has an excellent fertilizing effect on the obtained compost.

<Test 5: Examination of raw material for compost>
In the above test, a mixture of chicken manure and rice husks was used as the compost raw material, but other combinations of compost raw materials were also examined.

(Raw material for compost)
The following materials were used as raw materials for compost of cow dung sawdust compost (initial C/N ratio=30), and they were mixed well to be uniform.
Cow dung: 50 kg/ku Sawdust: 13.31 kg/ku (about 27 L)
Cow dung compost (inoculum): 0.1 kg/section

(Outline of test area)
Regarding test groups, total of 2 test groups using Euglena powder as a fermentation promoting material (0.01 kg/0.5 kg) and 2 test groups using additive-free and fermentation accelerator (compost waste bacterial bed 0.02 kg) There were 4 test areas (using 80L container).

The sample label of each test plot was as follows.
・Sample 1: No additives (no fermentation accelerator, no Euglena powder)
・Sample 2: 20 g of waste bacterial bed for compost
・Sample 3: 0.01 kg of Euglena
・Sample 4: Euglena 0.5kg

Each measurement was performed in the same procedure as the above test. The results are shown below.
(Transition of fermentation heat)
FIG. 8 shows the result of the temperature measurement inside the container of each test section. Even in the compost using cow dung and sawdust, as in the case of using chicken dung and rice husks, a clear promotion of fermentation was observed in the Euglena-added section (Sample 4, main raw material ratio 10%) (Fig. 9). Arrow). Next, to verify the maturity of the compost, a komatsuna seedling toxicity and growth test was conducted.

(Komatsuna seedling germination rate)
The results of germination rate measurement are shown in FIG. Two weeks after the start of fermentation, the Euglena-added plots (Samples 3 and 4) showed no difference in the germination rate compared to the target plot (tap water test plot), indicating that the compost maturation was progressing. It was suggested.

(Komatsuna seedling toxicity and growth test)
Significant growth promotion was observed in the Euglena-added plots (Samples 3 and 4) in both the seedling root length (Fig. 10) and the seedling aerial length (Fig. 11).

(Results of compost component analysis)
FIG. 12 shows the results of measuring the amounts of each component in the compost fermentation process. In the test group to which 10 g of Euglena was added, the amount of nitrite nitrogen (NO ₂ ) increased remarkably, and it was suggested that the addition of Euglena activated the fermentation of microorganisms and promoted the decomposition of organic matter to inorganic matter.

Claims (6)

A compost fermentation accelerator containing euglena that has been subjected to at least one of a drying treatment and a mechanical treatment . A method for producing compost, which comprises adding Euglena that has been subjected to at least one of a drying treatment and a mechanical treatment to a compost raw material to perform fermentation. A method for fermenting compost, which comprises adding Euglena that has been subjected to at least one of a drying treatment and a mechanical treatment to a compost raw material to perform fermentation. A composting material containing a compost raw material and euglena that has been subjected to at least one of a drying treatment and a mechanical treatment . A compost fermentation product of a compost raw material and euglena that has been subjected to at least one of a drying treatment and a mechanical treatment . A culture soil containing a compost fermentation product of a compost raw material and Euglena which has been subjected to at least one of a drying treatment and a mechanical treatment .

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