TWI605821B - A use of an extract of asplenium australasicum (j. sm.) hook. - Google Patents
A use of an extract of asplenium australasicum (j. sm.) hook. Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61P19/00—Drugs for skeletal disorders
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A—HUMAN NECESSITIES
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- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
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Description
本發明係關於一種南洋山蘇水萃物的用途，特別關於一種南洋山蘇水萃物應用於製備治療退化性關節炎的藥物的用途。 The invention relates to the use of a Nanyangshan Sushui extract, in particular to a use of a Nanyangshan Sushui extract for the preparation of a medicament for treating degenerative arthritis.
退化性關節炎(orteoarthritis，簡稱OA)為由關節軟骨的破壞(breakdown of joint cartilage)所引起的關節疾病(joint disease)，常見的症狀為關節的疼痛與僵硬(stiffness)。 Degenerative arthritis (orteaarthritis, hereinafter referred to as OA) is a joint disease caused by a breakdown of joint cartilage. Common symptoms are joint pain and stiffness.
臨床上係以被動方式抑制退化性關節炎的惡化或減緩患者的疼痛，例如減輕關節所受到之壓力(decrease joint stress)，及利用NSAID等藥物來減少關節的發炎症狀；或是利用外科手術治療，進行關節鏡清創術(arthroscopic debridement)以修補關節之破裂部位等；惟前述處置仍無法完全根治退化性關節炎。是以，確實有必要發展能夠治療退化性關節炎的藥物。 Clinically, it inhibits the deterioration of degenerative arthritis or slows down the pain of patients, such as reducing the joint stress, and using drugs such as NSAID to reduce inflammation of the joints; or using surgical treatment. Arthroscopic debridement is performed to repair the rupture site of the joint; however, the above treatment still cannot completely cure degenerative arthritis. Therefore, it is indeed necessary to develop drugs that can treat degenerative arthritis.
為解決上述問題，本發明提供一種南洋山蘇水萃物的用途，係將萃取自南洋山蘇之活性成分，應用於製備治療退化性關節炎的藥物者。 In order to solve the above problems, the present invention provides a use of a Nanyangshan Sushui extract, which is an active ingredient extracted from Nanyangshansu, and is used for preparing a medicament for treating degenerative arthritis.
本發明之南洋山蘇水萃物的用途，係應用於製備治療退化性關節炎的藥物，其中，該南洋山蘇水萃物係水於115~125℃之溫度、0.35~0.5Kg/cm2G之壓力下萃取1.5~3小時所獲得，較佳地，該南洋山蘇水萃物係能夠以口服方式投予一所需個體，且該南洋山蘇水萃物之每天投予 該所需個體之劑量為3~6毫克/公斤體重；如此藉由萃取自南洋山蘇之活性成分，可以減緩木瓜蛋白酶所誘發的退化性關節炎症狀，因而可以應用於製備治療退化性關節炎的藥物，為本發明之功效。 The use of the Nanyangshan Sushui extract of the invention is applied to the preparation of a medicament for treating degenerative arthritis, wherein the water of the Nanyangshan Sushui extract is at a temperature of 115-125 ° C, 0.35-0.5 Kg/cm 2 It is obtained by extracting under pressure of G for 1.5 to 3 hours. Preferably, the Nanyangshan Sushui extract can be administered orally to a desired individual, and the Nanyangshan Sushui extract is administered daily. The dosage of the individual is 3-6 mg/kg body weight; thus, by extracting the active ingredient from Nanyangshansu, the symptoms of degenerative arthritis induced by papain can be alleviated, and thus it can be applied to the preparation of a medicament for treating degenerative arthritis. It is the efficacy of the invention.
第1圖：試驗(A)中第A0~A2組大鼠於跑步機上持續跑步的時間。 Figure 1: The duration of continuous running on the treadmill in the A0~A2 group of rats in trial (A).
為讓本發明之上述及其他目的、特徵及優點能更明顯易懂，下文特舉本發明之較佳實施例，並配合所附圖式，作詳細說明如下：本發明所述之「南洋山蘇(Asplenium australasicum(J.Sm.)Hook.)」，係指鐵角蕨科(Aspleniaceae)鐵角蕨屬(Asplenium)的植物，其新生嫩葉(young emerging frond)常被作為食用菜葉。 The above and other objects, features and advantages of the present invention will become more <RTIgt; Asplenium australasicum (J.Sm.) Hook.) refers to the plant of the Asplenaceae Asplenium , whose young emerging frond is often used as a edible leaf.
本發明之南洋山蘇水萃物，係可以減緩木瓜蛋白酶所誘發的退化性關節炎症狀，因而可以應用於製備治療退化性關節炎的藥物，因而可以應用於製備治療退化性關節炎的藥物，該南洋山蘇水萃物與醫藥學上可以接受之載劑或賦形劑組合形成一醫藥組合物，其中，該南洋山蘇水萃物係可以製備成任何方便食用之型式，如錠劑、膠囊、粉劑、粒劑或液劑等，或者將該南洋山蘇水萃物與其他食品或飲料組合，以適於食用之樣態供生物體以口服方式服用。 The Nanyangshan Sushui extract of the invention can alleviate the symptoms of degenerative arthritis induced by papain, and thus can be applied to the preparation of a medicament for treating degenerative arthritis, and thus can be applied to the preparation of a medicament for treating degenerative arthritis. The Nanyangshan Sushui extract is combined with a pharmaceutically acceptable carrier or excipient to form a pharmaceutical composition, wherein the Nanyangshan Sushui extract can be prepared into any convenient form, such as a lozenge, Capsules, powders, granules or liquids, or the combination of the Nanyangshan Sushui extract with other foods or beverages, for oral administration to the organism for oral administration.
又，本發明之南洋山蘇水萃物係可以供投予一所需個體，例如以3~6毫克/公斤體重之劑量投予該所需個體，使該南洋山蘇水萃物係所含有之活性成分得以於該所需個體體內作用，而可以減緩木瓜蛋白酶所誘發的退化性關節炎症狀。 Further, the Nanyangshan Sushui extract of the present invention can be administered to a desired individual, for example, by administering the desired individual at a dose of 3-6 mg/kg body weight, so that the Nanyangshan Sushui extract system contains The active ingredient is allowed to act in the desired individual, and the symptoms of degenerative arthritis induced by papain can be alleviated.
其中，該南洋山蘇水萃物較佳係可以藉由一包含以下步驟之方法所製得：提供一南洋山蘇樣品；以水作為一萃取溶劑萃取該南洋山蘇 樣品；及將南洋山蘇粗萃液進行濃縮，以獲得該南洋山蘇水萃物。 Wherein, the Nanyangshan Sushui extract can be obtained by a method comprising the steps of: providing a sample of Nanyangshansu; extracting the Nanyangshansu with water as an extraction solvent; a sample; and concentrating the Nanyangshansu crude extract to obtain the Nanyangshan Sushui extract.
詳而言之，該南洋山蘇樣品係可以為南洋山蘇之成熟老葉(三年以上，背面分布孢子)，較佳係可以預先將該南洋山蘇樣品於110~120℃之溫度下進行乾燥(將100克南洋山蘇樣品乾燥至20克)，得到一南洋山蘇乾燥物；此外，該南洋山蘇樣品亦可以預先碎成粉粒(粒徑約為1~2mm)，以增加該南洋山蘇樣品與該萃取溶劑之接觸表面積，藉此提升後續萃取之萃取效率。 In detail, the sample sample of Nanyangshansu may be mature mature leaves of Nanyangshansu (more than three years, spores are distributed on the back), and it is preferable to carry out the sample of Nanyangshansu in advance at a temperature of 110-120 °C. Drying (100 g of Nanyangshansu sample is dried to 20 g) to obtain a dried Nanyangshan Su; in addition, the Nanyangshansu sample can also be pre-crushed into powder (having a particle size of about 1 to 2 mm) to increase the The contact surface area of the Nanyangshansu sample with the extraction solvent, thereby improving the extraction efficiency of the subsequent extraction.
舉例而言，每4.8公斤之南洋山蘇樣品係能夠混合該萃取溶劑，使二者總體積達80公升，並於115~125℃之溫度、0.35~0.5Kg/cm2G之壓力下進行萃取1.5~3小時，上述萃取亦可以重複數次，使該南洋山蘇樣品所富含之活性成分可以完整溶出於該萃取溶劑，此為本發明所屬技術領域中具有通常知識者所廣泛應用，在此不加以贅述。 For example, every 4.8 kg of Nanyangshansu sample can mix the extraction solvent to a total volume of 80 liters, and extract at a temperature of 115-125 ° C and a pressure of 0.35-0.5 Kg/cm 2 G. 1.5~3 hours, the above extraction may also be repeated several times, so that the active ingredient rich in the sample of Nanyangshansu can be completely dissolved in the extraction solvent, which is widely used by those having ordinary knowledge in the technical field of the invention. This will not be repeated.
前述之南洋山蘇粗萃液可以經過減壓濃縮及冷凍乾燥，以獲得該南洋山蘇水萃物，藉由此一程序，係可以使該南洋山蘇水萃物之活性成分更加濃縮，是以僅需使用少量之該南洋山蘇水萃物即可以發揮最佳療效。 The aforementioned Nanyangshan Su crude extract liquid can be concentrated under reduced pressure and freeze-dried to obtain the Nanyangshan Sushui extract. By this procedure, the active ingredient of the Nanyangshan Sushui extract can be more concentrated. The best effect can be achieved by using only a small amount of the Nanyangshan Sushui extract.
為證實本發明之南洋山蘇水萃物係可以減緩木瓜蛋白酶所誘發的退化性關節炎症狀，遂將每4.8公斤之南洋山蘇樣品萃取所得之南洋山蘇粗萃液，經過減壓濃縮及冷凍乾燥獲得共800克之南洋山蘇水萃物粉末，另將5克之南洋山蘇水萃物粉末溶於20毫升之水後，進行以下試驗。 In order to confirm that the Nanyangshan Sushui extract of the present invention can alleviate the symptoms of degenerative arthritis induced by papain, the crude extract of Nanyangshansu, which is extracted per 4.8 kg of Nanyangshansu sample, is concentrated under reduced pressure. A total of 800 g of Nanyangshan Sushui extract powder was obtained by freeze-drying, and 5 g of Nanyangshan Sushui extract powder was dissolved in 20 ml of water, and the following test was carried out.
請參照第1表所示，本試驗係選用SD大鼠(Sprague Dawley rat)進行試驗，係於右膝關節(knee joint)注射木瓜蛋白酶(papain)，以誘發退化性關節炎的發生。 Please refer to the first table. This test was performed using SD rats (Sprague Dawley rat), and the knee joint was injected with papain to induce the development of degenerative arthritis.
續分別以如第1表所示之南洋山蘇水萃物連續經口灌食各組大鼠共3個月(每天一次)後，讓各組大鼠於跑步機(treadmill machine) 上跑步(跑步機的速度為16m/s)，並記錄各組大鼠持續跑步的時間。 Continue to use the Nanyangshan Sushui extract as shown in Table 1 to continuously ingest the rats in each group for 3 months (once a day), and let each group of rats on the treadmill machine. Run up (treadmill speed 16m/s) and record the duration of running in each group of rats.
請參照第1圖所示，相較於未經口灌食南洋山蘇水萃物的第A0組大鼠，經口灌食濃度為3~6mg/kg的南洋山蘇水萃物的第A1、A2組大鼠均可以於跑步機上持續跑步較長的時間，顯示本發明南洋山蘇水萃物的投予可以有效降低木瓜蛋白酶所誘發的退化性關節炎症狀，使大鼠可以於跑步機上持續跑步較長的時間。 Please refer to Figure 1 for the A1 of the Nanyangshan Sushui extract with oral feeding concentration of 3~6mg/kg compared with the A0 group of rats without oral filling of Nanyangshan Sushui extract. The rats in group A2 can continue running for a long time on the treadmill, indicating that the administration of the Nanyangshan Sushui extract of the present invention can effectively reduce the symptoms of degenerative arthritis induced by papain, so that the rats can run The machine continued to run for a long time.
綜合上述，本發明之南洋山蘇水萃物的用途係藉由萃取自南洋山蘇之活性成分，可以減緩木瓜蛋白酶所誘發的退化性關節炎症狀，因而可以應用於製備治療退化性關節炎的藥物，為本發明之功效。 In summary, the use of the Nanyangshan Sushui extract of the present invention can slow down the symptoms of degenerative arthritis induced by papain by extracting the active ingredient from Nanyangshansu, and thus can be applied to the preparation of the treatment of degenerative arthritis. The drug is the efficacy of the present invention.
雖然本發明已利用上述較佳實施例揭示，然其並非用以限定本發明，任何熟習此技藝者在不脫離本發明之精神和範圍之內，相對上述實施例進行各種更動與修改仍屬本發明所保護之技術範疇，因此本發明之保護範圍當視後附之申請專利範圍所界定者為準。 While the invention has been described in connection with the preferred embodiments described above, it is not intended to limit the scope of the invention. The technical scope of the invention is protected, and therefore the scope of the invention is defined by the scope of the appended claims.
Claims (3)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW106101439A TWI605821B (en) | 2017-01-16 | 2017-01-16 | A use of an extract of asplenium australasicum (j. sm.) hook. |
| PCT/US2017/068408 WO2018132255A1 (en) | 2017-01-16 | 2017-12-26 | Method for treating osteoarthritis |
| CN201780083325.2A CN110198725A (en) | 2017-01-16 | 2017-12-26 | The method for treating degenerative arthritis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW106101439A TWI605821B (en) | 2017-01-16 | 2017-01-16 | A use of an extract of asplenium australasicum (j. sm.) hook. |
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| Publication Number | Publication Date |
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| TWI605821B true TWI605821B (en) | 2017-11-21 |
| TW201827062A TW201827062A (en) | 2018-08-01 |
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| TW106101439A TWI605821B (en) | 2017-01-16 | 2017-01-16 | A use of an extract of asplenium australasicum (j. sm.) hook. |
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| Country | Link |
|---|---|
| CN (1) | CN110198725A (en) |
| TW (1) | TWI605821B (en) |
| WO (1) | WO2018132255A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018226447A1 (en) * | 2017-06-06 | 2018-12-13 | Jin Xin Biotechnology Co., Ltd. | Method for inhibiting inflammation caused by gram-negative bacteria infection |
| CN110198725A (en) * | 2017-01-16 | 2019-09-03 | 景鑫生物科技股份有限公司 | The method for treating degenerative arthritis |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020132021A1 (en) * | 1997-04-30 | 2002-09-19 | Ilya Raskin | Elicited plant products |
| US20040175439A1 (en) * | 2001-03-02 | 2004-09-09 | Benoit Cyr | Plant extracts and compositions comprising extracellular protease inhibitors |
| CN105497480A (en) * | 2015-12-21 | 2016-04-20 | 王得帅 | Traditional Chinese medicine for treating knee osteoarthritis |
| TWI605821B (en) * | 2017-01-16 | 2017-11-21 | 景鑫生物科技股份有限公司 | A use of an extract of asplenium australasicum (j. sm.) hook. |
-
2017
- 2017-01-16 TW TW106101439A patent/TWI605821B/en not_active IP Right Cessation
- 2017-12-26 WO PCT/US2017/068408 patent/WO2018132255A1/en active Application Filing
- 2017-12-26 CN CN201780083325.2A patent/CN110198725A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| Lih-Shiuh Lai et al,"Chemical compositions and some physical properties of the water and alkali-extracted mucilage from the young fronds of Asplenium australasicum (J. Sm.) Hook", Food Hydrocolloids, 2012, 26(2): 344-349. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110198725A (en) * | 2017-01-16 | 2019-09-03 | 景鑫生物科技股份有限公司 | The method for treating degenerative arthritis |
| WO2018226447A1 (en) * | 2017-06-06 | 2018-12-13 | Jin Xin Biotechnology Co., Ltd. | Method for inhibiting inflammation caused by gram-negative bacteria infection |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110198725A (en) | 2019-09-03 |
| TW201827062A (en) | 2018-08-01 |
| WO2018132255A1 (en) | 2018-07-19 |
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