TWI571268B - The formula and method of whitening and moisturizing skin care products - Google Patents

Description

Formulation and preparation method of whitening and moisturizing skin care products

The invention relates to a formula and a process for whitening and moisturizing skin care products, in particular to a formulating technology and a manufacturing technology for preparing a beauty care product and providing a good beauty maintenance function.

According to the conventional electrospinning technology, the principle of positive and negative phase attraction of electric field is adopted. The polymer solution is placed in a syringe and ejected through a metal needle. The initial semicircular drop is subjected to a high voltage electric field and the shape is changed to form a Taylor cone ( The appearance of the Taylor cone), while jetting a micron-sized jet, the diameter of the liquid column gradually spreads as the distance from the needle is extended, and finally spreads out to form many finer nanometers. The liquid column is used, and the solvent is more volatile because the liquid column is thinner. Finally, the micro-nanofilament fiber can be collected on the grounded collector plate. For the technique, refer to Reference 1.

The known hyaluronic acid (Hyaluronan, HA) was first isolated from the vitreous humor of the eye in 1934 and contained uronic acid and amino sugar. The substance is named hyaluronic acid (see Reference 2), which is currently recognized as the best moisturizing ingredient, also known as uronic acid, hyaluronic acid, and glacial sugar carbonic acid. Hyaluronic acid HA is a high-grade polysaccharide composed of D-glucuronic acid and N-acetyl-D-glucosamine disaccharide units, which is a primary structure. . The monosaccharides are linked to each other via a β-1,3 glycosidic bond, and the disaccharide units are linked to each other by β-1,4 glycosidic bonds. Repeatedly form a chain polymer, which is a secondary structure (see Reference 3). The hyaluronic acid molecule contains a large amount of carboxyl groups and hydroxyl groups, which can form intramolecular and intermolecular hydrogen bonds (each disaccharide unit can form four hydrogen bonds), so the disaccharide unit will be formed parallel to the molecular axis chain. The hydrogen bond is combined, the inner side of the column is strongly hydrophilic due to the presence of a large amount of hydroxyl groups, and the hyaluronic acid molecule locks the water molecules it binds in its double helix columnar structure, so it can absorb and maintain it more than 500 times. The moisture makes it easy to lose moisture and has a special water retention effect. However, because the carboxyl groups in between are mutually repelled, and many irregular local helices (Helix) are generated, occupying a large amount of space, a hydrophobic region exists in the hyaluronic acid molecule, and the hyaluronic acid molecules are completely chain-entangled to form a network. The tertiary structure. Hyaluronic acid has special biological activity and possesses non-toxicity, low immune response, high biocompatibility, biodegradability and absorption of the human body. Therefore, it can be used in cosmetics, health food, and medical fields, for example, as Humectants, oral hyaluronic acid, eye surgery, intra-articular injection, wound healing, surgical anti-adhesive agents (see Reference 4).

Arbutin is a derivative of hydroquinone, commonly known as melanin, which is extracted from the bearberry leaves of the bilberry family. The inhibitory effect of arbutin on melanin formation is to reduce the activity of tyrosinase by competitive blocking, and to inhibit the formation of melanin. Reference 5 is the whitening ingredient approved by the Department of Health for use in cosmetics. The use limit is 7%, hydroquinone should not be incorporated, and the impurities contained in the product must be less than 20 ppm. In the whitening mechanism, one of the main factors affecting skin color is melanin pigment, and the melanin pigment is synthesized by the melanine of melanin pigment cells (see Reference 6). ). Melanin is formed by the action of Tyrosine and tyrosinase in the body to form Dopa, and then tyrosinase to form Dopaquinone, and then form a red skin type. Intermediate product Pheomelanin and intermediates of black, white and Asian skin color Eumelanin, finally forming mixed type melanins (see Reference 7).

By inhibiting or destroying the formation of melanin by whitening ingredients, it is possible to reduce the formation of melanin and achieve a skin whitening effect. The mechanism of action of whitening ingredients includes inhibiting the formation of melanin in melanocytes, reducing the old melanin in the body, and promoting the metabolism of melanin in the epidermis. The way to inhibit the formation of melanin can be further divided into: (1) inhibition of the synthesis of tyrosinase. (2) Prevent tyrosinase activation. (3) Competitive substitution of tyrosine enzyme substrate. (4) Blocking the intermediate of melanin melanin synthesis. See Reference 8, the principle of arbutin whitening is to use a competitive substitution of tyrosinase, which competes with tyrosine for the binding site of tyrosinase activity, resulting in decreased activity of tyrosinase, which hinders tyrosine The amine acid is further oxidized to dopa and dopaquinone to achieve the effect of inhibiting melanin formation (see Reference 9).

Mandelic Acid, also known as mandelic acid and phenylglycolic acid, has a chemical formula of C8H8O3. It can be used alone or in combination with antioxidant vitamins. It has a variety of beneficial effects on the treatment of skin, including antibacterial effects and improving photoaging skin, acne. , pigmentation abnormalities, and skin texture, see reference 10.

During the manufacture and use of cosmetics, it is inevitable to be contaminated by microorganisms such as bacteria and mold in the air. Therefore, antibacterial agents must be added to cosmetics. In this study, natural grapefruit seeds were used as antibacterial agents to reduce skin allergies. Grapefruit seed extract is a liquid extracted from grapefruit pulp and seeds. It has antibacterial effect (see Reference 11). It is an aqueous phase antibacterial agent that can be added to foods and cosmetics. It is a safe, A natural, effective antibacterial agent. Its antibacterial, antiviral and antifungal properties (see Reference 12) have led some physicians to use it as an alternative medicine for the treatment of candidiasis, earache, throat infections and diarrhea.

Most women always carry many bottles and cans of skin care products when they travel, which not only takes up space, but also causes inconvenience in storage. Currently, the market is covered by a cotton pad. Among them, most of the cotton pads are cotton, wood pulp, non-woven fabrics and other disposable materials, which are thrown away after use, and have a considerable burden on environmental pollution. In order to solve the above-mentioned shortcomings, the present inventors have actively invested in research and development with years of rich research and design experience, and through continuous research, implementation, and experimentation, the final development has created the combination of Arbutin and Hyaluronic Acid. A new solid beauty care product with whitening, moisturizing, antiseptic and antibacterial properties. Moreover, by applying electrospinning technology, it can be directly made into a nano-fibrous fiber film, which can be directly absorbed by the skin during use, and achieves the purpose of reducing the amount of garbage, reducing the packaging, facilitating carrying and function.

A first object of the present invention is to provide a formula and a process for whitening and moisturizing skin care products. The technical method comprises dissolving a cosmetic formula material comprising at least one of hyaluronic acid, arbutin, mandelic acid and grapefruit seed antibacterial agent in water, and using the processing technology to form the cosmetic formula material solution into a beauty care product.

A second object of the present invention is to provide a formula and a process for whitening and moisturizing skin care products. The technical method is prepared by dissolving a cosmetic formula material comprising at least one of hyaluronic acid, arbutin, mandelic acid and grapefruit seed antibacterial agent in water, and spinning the cosmetic formula material solution by electrospinning technology. Fiber film, used as a facial mask or skin mask for beauty whitening and moisturizing.

10‧‧‧Injection device

11‧‧‧Nozzles

20‧‧‧ collecting board

30‧‧‧Fiber

Figure 1 is an electrospinning technology device of the present invention; 2 is the result of the moisturizing efficacy test of the spinning solution of the present invention; FIG. 3 is the result of measuring the potency of tyrosinase inhibition, K represents Kojic acid, and the concentrations are 10 mM and 1 mM, respectively, as positive control group; A represents arbutin The concentration of Arbutin was 0.18 and 0.04 mM, respectively, as a control group; Figure 4 is a comparison chart of the antibacterial efficacy test results of the spinning solution of the present invention, S represents Staphylococcus aureus, E represents large bacillus, and P represents Pseudomonas aeruginosa; The result of the moisturizing performance test of the finished product of the present invention; FIG. 6 is the result of the dry finished whitening efficacy test of the present invention, wherein K represents Kojic acid, and the concentrations thereof are 10 mM and 1 mM, respectively, as a positive control group; A represents a concentration of 0.18, respectively. And 0.04 mM Arbutin as a control group; FIG. 7 is a schematic diagram of a formula comparison table of the present invention; and FIG. 8(A) is a moisturizing liquid formulation of Table 1 (b), (d) and (e) of the present invention. Test for S. aureus; Figure 8 (B) shows the test for E. coli in the moisturizing liquid formulations of Tables 1 (b), (d) and (e); Figure 8 (C) is the present invention The test for Pseudomonas aeruginosa was carried out with the moisturizing liquid formulations of (b), (d) and (e) of Table 1; The electrospinning solution has a flow rate of 20 μl/min and a spinning time of 60 min. The SEM photograph of the prepared film; the (a) film has a spinning electric field of 15 kV/8 cm; and (b) is a partial enlargement of the figure (a). Figure (c) The spinning electric field of the film is 15KV/10cm; Figure 10 is the electrospinning dry product of the present invention; the sample (1) of Figure 11 is the formulation (b) of Figure 7 but the grapefruit seed antibacterial agent is removed. Electrospinning dry finished product, sample (2) is formulation (b) electrospinning dry finished product manufactured by electrospinning technology, sample (3) is formulation (b) film produced by casting method; figure (A) is Each sample of the invention against golden yellow grapes The inhibition test sample of cocci is shown; (B) is the inhibition test of Escherichia coli for each sample of the present invention; (C) is the inhibition test of Pseudomonas aeruginosa for each sample of the present invention; FIG. 12(A) is the invention of the present invention. The finished product is packaged with aluminum foil; Figure 12 (B) is the finished package of the present invention, which is sealed with a zipper bag.

The technical feature of the present invention is to combine the maintenance ingredients such as Arbutin, Hyaluronic Acid and Mandelic Acid with polyethylene glycol (PEO) and grapefruit seed antibacterial agent by electrospinning technology. The product formulation is made into a dry fiber film skin care product with whitening antibacterial function with a spinning solution flow rate of 20 μl/min, an electric field of 15 kV/8 cm, and a spinning time of 60 min.

The experimental materials of the experimental examples of the present invention were hyaluronic acid, HA (MW=1440,000), Arbutin (MW=272.25), mandelic acid (MW=152.14), polyethylene oxide ( Polyethylene oxide, PEO) (MW=900,000), grapefruit seed antibacterial agent, round paper tray paper disc (diameter 8mm), tryptic Soybean Broth (TSB) liquid medium, tryptone soy agar (Tryptone) Soy agar, TSA) Dry medium, without pretreatment before use. 50 mM sodium phosphate buffer, adjusted to pH 6.8 with 0.01 N sodium hydroxide NaOH before use, 2 mM 3,4-dihydroxyphenylalanine (L-DOPA), 100 U/ml tyrosinase Tyrosinase 10 mM and 1 mM citric acid (Kojic acid) were prepared before the experiment, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and cultured for 12 hours.

The experimental procedure of the experimental example of the present invention is first Preparation of a cosmetic moisturizing liquid formulation. The percentage of hyaluronic acid added in general cosmetics is 0.1% to 0.5%. This formula is designed to add hyaluronic acid 0.2% and 0.3% for moisturizing ability comparison. The arbutin was announced in the Department of Health to use a limit of 7%. To avoid high levels of allergies, the formula 1% and 5% of the formula were compared for tyrosinase inhibition. While bitter almond acid belongs to fruit acids, it may also cause allergies due to high concentration, so the design is added 1%. Polyethylene oxide PEO needs to be more than 5% to form a film through electrospinning technology. In the formulation of this experimental example, 7% is added, mainly as a film-forming agent. Grapefruit seeds only need 0.5% to have a significant antibacterial effect, while more than 3% will precipitate in the formula, so the formula design 0.5%, 1%, 2% for antibacterial ability comparison.

The present invention performs an evaluation of the effectiveness of a cosmetic moisturizing liquid formulation, that is, a skin short-acting moisturizing ability test. In the experimental examples, the tests were carried out using the formulations (a) and (b) shown in Fig. 7 as samples. The procedure is as follows: the test conditions are relative humidity of 45±5%, and room temperature is 25±3°C; after washing with both hands, the skin moisture content of the fixed position inside the wrist is measured by the skin mass meter; after the measurement, the experimental group is quickly applied with 100 μl. The solution was measured, and the control group was allowed to stand; the skin water content was measured by skin mass measurement every 10 minutes, and each test was performed 3 times, and the average value was continuously measured for 90 minutes; and the result data was graphed to observe the moisturizing efficiency.

The invention carries out an in vitro test of the whitening effect of the cosmetic moisturizing liquid formula, that is, the tyrosinase inhibition performance test. In the experimental examples, the tests were carried out using the formulations (b) and (c) of Fig. 7 as samples. The procedure is as follows: 140 μl of 3,4-dihydroxyphenylalanine L-DOPA at a concentration of 2 mM is added to a 96-well plate; 40 μl of the sample solution is added; and the positive control group is substituted with a concentration of 10 mM and 1 mM of citric acid Kojic acid; The negative control group was replaced with sodium phosphate buffer (pH 6.8) at a concentration of 50 mM; 20 μl of 100 U/ml tyrosinase Tyrosinase was added; the reaction was protected from light for 30 minutes at 25 ° C; the absorbance of OD ₄₇₅ was measured; The inhibition rate of the sample to tyrosinase was calculated; and the results were plotted against the data and the ability to inhibit tyrosinase was determined.

The present invention conducts a safety evaluation of a cosmetic moisturizing liquid formulation, that is, an antiseptic efficacy test, and conducts an experiment using an agar disc diffusion method. In the experimental examples, the tests were carried out using the formulations (b), (d), and (e) shown in Fig. 7 as samples. The steps include: taking Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa cultured in Tryptone soy agar (TSA) medium (including: Tryptone 15g/l, Soytone or Thiopeptone or Thiotone 5g/l, Sodium chloride (NaCl 5g / l, Agar) 12 hours of bacteria; the three bacteria liquid individually measured the absorbance of OD ₅₉₅ , the OD value is controlled in the range of 0.7 ~ 0.8; take 200μl of bacterial solution, applied to trypsin Tryptic Soybean Broth (TSB) dry medium (containing: tryptone Tyrptone 17g / l, soybean extract Soybean Extract 3g / l, dipotassium hydrogen phosphate K ₂ HPO ₄ 2.5g / l, sodium chloride NaCl 5g / l, glucose Dextrose 2.5g / l), each application of three plates, a total of nine plates; take antibacterial ring patch, dilute different concentrations of the test solution, respectively, attached to the dry medium; placed in a 37 ° C incubator Incubate for 12 hours; and observe the size of the inhibition loop to determine its antibacterial efficacy.

The production of the dry cosmetic moisturizing fiber film of the present invention. Electrospinning experimental parameters: flow rate of electrospinning solution, electric field (voltage and working distance). The solution is sprayed on the metal collecting plate 20 through the nozzle 11 of the spraying device 10 under the action of electric field spinning, and a general photocopying paper is attached to the surface of the collecting plate 20. The static electricity will adsorb the fibers 30 to the surface of the paper to form a staggered net. Structure. This method must control the concentration of the drawing solution, the flow rate of the injection solution, the distance (d) of the nozzle 11 to the collecting plate 20, and the applied voltage (V), as shown in Fig. 1, because these factors affect the spinning fiber 30. The average diameter. Then observe the diameter and pore size of the fiber with an electron microscope. The most suitable electrospinning experimental parameters, the finished product.

The present invention carries out an evaluation of the effectiveness of a dry cosmetic moisturizing fiber film, that is, a skin short-acting moisturizing ability test. The test method is roughly the same as the previous evaluation of the effectiveness of the beauty moisturizing liquid formula-skin short-acting moisturizing ability test. Only after the step 3 is changed, the experimental group takes a 15x15mm dry type beauty moisturizing fiber film and attaches it to the inner side of the wrist. Fixed position; control group was allowed to stand.

The invention carries out an in vitro test for the whitening effect of the dry cosmetic moisturizing fiber film, that is, the tyrosinase inhibition performance test. The test method was roughly the same as the in vitro test of the whitening effect of the previous cosmetic moisturizing liquid formula, that is, the tyrosinase inhibition efficacy test, and only step 2 was changed to dissolve the 0.04 g dry beauty moisturizing film into 40 μl of the buffer solution.

The present invention performs a safety evaluation of a dry cosmetic moisturizing fiber film, that is, an anticorrosive efficacy test (patch test). The test method is roughly the same as the safety evaluation of the previous cosmetic moisturizing liquid formula, that is, the anti-corrosion efficacy test (patch test), and only the steps 3 and 4 are directly applied to the 15×15 mm dry beauty moisturizing fiber film respectively. on.

The dry cosmetic moisturizing fiber film of the present invention is packaged in an outer layer. The dry beauty moisturizing fiber film is coated with aluminum foil, sealed with a plastic PE film, and designed with a zipper bag seal, which can accommodate multiple dry skin care products.

The experimental results of the present invention show the effectiveness evaluation of the cosmetic moisturizing liquid formula - the skin short-acting moisturizing ability test result: the skin short-acting moisturizing ability is measured, and the skin is short in the formula (a) and (b) of Fig. 7. The experimental test of effective moisturizing ability, as shown in Figure 2, shows that the skin moisture content reaches a maximum within 10-20 minutes, but gradually decreases after 20 minutes. Finally, the skin of formula (a) The skin moisture rate is 32.7%, and the skin moisture rate of the formula (b) is 34.3%. The formula (b) has a better moisturizing effect.

The experimental results of the present invention show the whitening effect of the beauty moisturizing liquid formula in vitro test-tyrosinase inhibition efficacy test: the inhibitory effect of arbutin on tyrosinase, the experiment is tested by formulas (b) and (C), As a result, as shown in Fig. 3, the inhibition rate of the formula (b) to tyrosinase was 18.49%, and the inhibition rate of the formula (C) to tyrosinase was 13.20%, and it was found that the whitening effect of the formula (b) was better.

The experimental results of the present invention show the safety evaluation of the cosmetic moisturizing liquid formulation - antiseptic efficacy test (tested by agar disc diffusion method: Fig. 8 is shown in Fig. 7 (b), (d) And (e) formula for the evaluation of the anti-corrosion and antibacterial efficacy of the cosmetic moisturizing liquid, wherein the figures (A), (B), and (C) are the test results of the test for Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, respectively. The size of each of the antibacterial rings, we found that the beauty moisturizing liquid formula has a better inhibitory effect on Staphylococcus aureus, the inhibition ring is greater than 17.78mm, and the inhibition effect on Pseudomonas aeruginosa is poor, the inhibition ring is about Compared with 10.00mm, the antibacterial ring of formula (b) is larger and the antibacterial effect is better, while the antibacterial ring of formula (e) is the smallest and the antibacterial effect is the worst.

The invention is applied to the production of dry fiber films. From the above experimental results, it can be concluded that the formula (b) of Fig. 7 has a better effect in the moisturizing, whitening, and antibacterial tests, and therefore the formula (b) is used for the electrospinning technique to produce a fiber membrane. Fig. 9(a) and Fig. 9(b) are SEM photographs of the film prepared by the spinning solution flow rate of 20 μl/min, electric field of 15 kV/8 cm, and spinning time of 60 min, with obvious fiber structure and fiber thickness. More consistent. 9(b) is a partial enlargement of FIG. 9(a), and it can be clearly seen that the fiber diameter is about 600 nm or less. Annex IV is its finished product. And Figure 9(c) is The spinning solution has a flow rate of 20 μl/min, a spinning electric field of 15 kV/10 cm, and a spinning time of 60 min. The SEM photograph of the film produced is relatively discontinuous and uneven in thickness.

Evaluation of the effectiveness of dry beauty moisturizing fiber film - skin short-acting moisturizing ability test. Figure 5 is a review of the effectiveness of the skin short-acting moisturizing ability of the electrospun dry product. Among them, the blank group was tested without washing any solution after washing the hands, and the experimental group was tested after covering the finished product after washing the hands. The results showed that the skin moisture rate of the blank group decreased from 40.7% at 0 min to 32.7% at 90 min, a decrease of 19.6%. The skin moisture rate of the experimental group (electrospun dry fiber finished product) was 41.0% at 0 min. It fell to 35.7% at 90min, which was about 14.8%. The moisturizing effect of the electrospun dry finished product is obviously better.

Whitening efficacy of dry beauty moisturizing fiber film in vitro test - tyrosinase inhibition efficacy test. In order to study the inhibitory effect of the dry cosmetic moisturizing fiber film on tyrosinase, 120 mg of the dry finished product was dissolved in a buffer solution of 120 μl as a sample solution before the test, and the test was started as shown in the experimental step (III). The experimental results are shown in Fig. 6. The inhibition rate of the dry product to tyrosinase is 24.13%, which is compared with the inhibition rate of tyrosinase of 18.49% for the formulation solution of Fig. 7 (b). The effect of the finished product on tyrosinase inhibition is better than the liquid formulation.

Safety evaluation of dry beauty moisturizing fiber film - anti-corrosion efficacy test (patch test). In order to understand the antiseptic effect of the electrospinning dry product, if the dry product is applied to the medium, it will immediately dissolve into a liquid state, and when it is taken or placed on the table top, the solution will flow due to shaking, so that the bacteriostatic area cannot be accurately measured. Fig. 11 (A) (B) (C) shows the results of an antiseptic and bacteriostatic test of S. aureus, Escherichia coli, and Pseudomonas aeruginosa, respectively, of the electrospun dry fiber product. Sample (1) is the formulation of Figure 7 (b) but the electrospinning dry product of the grapefruit seed antibacterial agent is removed, sample (2) For the formulation (b) of the electrospun dry product manufactured by the electrospinning technique, the sample (3) is the film of the formulation (b) casted. As can be seen from Fig. 11, the electrospinning film added with the antibacterial agent (grape seed) has no bacteriostatic effect, and the two films made of the grapefruit seed antibacterial agent have antibacterial function.

Dry beauty moisturizing fiber film is packaged outside. After removing the dry nanofiber film on the electrospinning collecting plate, place it on the cut aluminum foil paper as shown in Fig. 12(A), wrap it in a transparent PE zipper bag, and seal it in a sealing machine. The final product is shown in Figure 12(B).

Conclusion: The present invention utilizes electrospinning technology, combined with Arbutin, Hyaluronic Acid, and Mandelic Acid to prepare a dry nanofiber film, which is used when used. Skin surface temperature and ambient humidity dissolve the film to make it absorb the skin. The invention prepares the beauty moisturizing liquid solution by electrospinning technology, the spinning liquid flow rate is 20 μl/min, the spinning electric field is 15 kV/8 cm, and the spinning time is 60 min, and the nano-fiber can be manufactured. From the results of the measurement of the short-acting moisturizing ability of the skin, it was found that the skin moisture content of 35.7% was maintained after the dry preparation was made by the electrospinning technique for 90 minutes and the tyrosinase inhibition rate was 24.1%. The whitening and moisturizing skin care product made by electrospinning technology can not only enhance the moisturizing and whitening effect, but also has excellent suppression effect on Staphylococcus aureus, and has the advantages of being light and easy to carry, and can be dissolved immediately when used. Based on the above functional evaluation, whitening and moisturizing skin care products have excellent application potential in the cosmetics market in the future.

The above is only a possible embodiment of the present invention, and is not intended to limit the scope of the patents of the present invention, and the equivalent implementations of other changes according to the contents, features and spirits of the following claims should be It is included in the patent of the present invention. The invention is specifically defined in the structural features of the request item, is not found in the same kind of articles, and has practicality, advancement and industrial utilization. Sex, has met the requirements of the invention patent, and filed an application in accordance with the law. I would like to ask the bureau to approve the patent in accordance with the law to protect the legitimate rights and interests of the applicant.

references

[1] D.S. Young, Hyaluronic Acid-based Nanofibers via Electrospinning, North Carolina State University, Materials science & Engineering, master thesis, 15-16 (2006).

[2] K. Meyer, J.W. Palmer, The polysaccharide of vitreous humor. Journal of Biological Chemistry, 107, 629-634 (1934).

[3] Liu Kunhan, The effect of different nitrogen sources on the production of hyaluronic acid by streptococcus fermentation, National Cheng Kung University, Department of Chemical Engineering, Master thesis, 2-3 pages (2006).

[4] Zhang Yaozhong, Extraction of Glucosamine Derivatives and Application of Biomedical Materials, Datong University, Institute of Bioengineering, Master thesis, 36-37 (2008).

[5] Xiao Yimiao, Vitamin C Glycoside and Arbutin for the inhibition of melanin production by B16 melanoma cells, National Tsinghua University, Department of Chemical Engineering, Master Thesis, 18-19 (2007).

[6] Zhang Liqing, New Theory of Modern Cosmetics, Gao Li Press, 150-151 (2009).

[7] Wang Ajing, Development of HPLC Analysis Method for Cosmetic Whitening Ingredients, Chaoyang University of Science and Technology, Department of Applied Chemistry, Master Thesis, 2-7 (2004).

[8] Hong Weizhang, Li Jinzhi, Chen Rongxiu, cosmetic raw materials and functions, Yixuan Book Publishing House, 250 pages (2005).

[9] Qiu Jialing, Xu Zhaocheng, tyrosinase inhibition kinetics of three whitening agents, Journal of Hongguang 54, pp. 118-120 (2009).

[10] B. Mark, M.D. Taylor, Summary of Mandelic Acid for the improvement of Skin Conditions, Cosmetic Dermatology, 26-28 (1999).

[11] L. Reagor, J. Gusman, L. McCoy, E. Carino, and JP Heggers, The Effectiveness of Processed Grapefruit-Seed Extract as An Antibacterial Agent: I. An In Vitro Agar Assay, J. Altern. Complement Med , 8(3), 325-332 (2002).

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Claims (3)

The invention relates to a method for preparing a whitening and moisturizing skin care product, which is prepared by dissolving a beauty formula material in water, and by using an electrospinning technology to spin the beauty formula material into a fiber film to form a cosmetic maintenance mask, the cosmetic formula material comprises a transparent material. Acid, arbutin, amygdalin and grapefruit seed antibacterial agent, wherein the cosmetic formula comprises 0.3 g of hyaluronic acid, 1 g of arbutin, 1 g of amygdalin, PEO 7 g and grapefruit seed antibacterial agent 1 g per 100 g of water. The electrospinning technology has a spinning solution flow rate of 20 μl/min, an electric field of 15 kV/8 cm, and a spinning time of 60 minutes to prepare the fiber film; when used, the fiber is exposed to human skin surface temperature and environmental humidity. The film dissolves and is absorbed by the skin. The method for producing a whitening and moisturizing skin care product according to claim 1, wherein the fiber has a diameter of about 600 nm or less. A cosmetic maintenance mask prepared according to the method of claim 1, wherein the formulation material is spin-formed into a fiber film by the electrospinning technique, and the fiber film is used as a cosmetic maintenance mask.