TWI564558B - 用於判定哺乳類動物細胞的心臟發展潛能之方法以及用於執行該方法之裝置 - Google Patents

用於判定哺乳類動物細胞的心臟發展潛能之方法以及用於執行該方法之裝置 Download PDF

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TWI564558B
TWI564558B TW099116051A TW99116051A TWI564558B TW I564558 B TWI564558 B TW I564558B TW 099116051 A TW099116051 A TW 099116051A TW 99116051 A TW99116051 A TW 99116051A TW I564558 B TWI564558 B TW I564558B
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genes
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安得利 德爾日克
阿達 培發爾
羅蘭 戈登貝爾斯福特
文錫安 高生
克斯秦 豪姆西
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馬友教育研究基金會
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TW099116051A 2009-05-20 2010-05-20 用於判定哺乳類動物細胞的心臟發展潛能之方法以及用於執行該方法之裝置 TWI564558B (zh)

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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2532909T3 (es) 2004-07-30 2015-04-01 Mayo Foundation For Medical Education And Research Tratamiento de tejido cardiovascular
US9765298B2 (en) 2006-07-24 2017-09-19 Mayo Foundation For Medical Education And Research Methods and materials for providing cardiac cells
WO2009145761A1 (en) 2008-05-27 2009-12-03 Mayo Foundation For Medical Education And Research Methods and materials for using cells to treat heart tissue
US9453205B2 (en) * 2009-10-31 2016-09-27 Genesis Technologies Limited Methods for reprogramming cells and uses thereof
AU2010312240B2 (en) * 2009-10-31 2016-12-15 Genesis Technologies Limited Methods for reprogramming cells and uses thereof
US20140038291A1 (en) 2009-10-31 2014-02-06 New World Laboratories Inc. Methods for reprogramming cells and uses thereof
US9185690B2 (en) * 2012-02-29 2015-11-10 Sharp Kabushiki Kaisha Allocating and determining resources for a device-to-device link

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI226440B (en) * 2000-09-08 2005-01-11 Kuo Kwang Biotech Corp Predicting survival of patients with squamous cell carcinoma

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020061837A1 (en) 1996-09-05 2002-05-23 Lough John W. Bone morphogenetic protein and fibroblast growth factor compositions and methods for the induction of cardiogenesis
US6324479B1 (en) * 1998-05-08 2001-11-27 Rosetta Impharmatics, Inc. Methods of determining protein activity levels using gene expression profiles
US6218122B1 (en) * 1998-06-19 2001-04-17 Rosetta Inpharmatics, Inc. Methods of monitoring disease states and therapies using gene expression profiles
US7547674B2 (en) * 2001-06-06 2009-06-16 New York Medical College Methods and compositions for the repair and/or regeneration of damaged myocardium
IL159580A0 (en) 2001-07-12 2004-06-01 Geron Corp Cells of the cardiomyocyte lineage produced from human pluripotent stem cells
AU2003220562A1 (en) * 2002-04-01 2003-10-20 Peter K. Law Cellular transplantation for heart regeneration
WO2004094610A2 (en) * 2003-04-21 2004-11-04 Baylor College Of Medicine Wnt as a factor for cardiac myogenesis
BRPI0414961A (pt) * 2003-10-03 2006-11-07 Keiichi Fukuda processo de indução da diferenciação de células tronco em células do miocárdio
DE10347436B4 (de) * 2003-10-13 2007-08-02 Johann Wolfgang Goethe-Universität Frankfurt am Main In vitro Verfahren zur Diagnose der kardiovaskulären Funktionalität von Blut-abgeleiteter zirkulierender Vorläuferzellen (BDP)
US20070077578A1 (en) * 2004-03-02 2007-04-05 Primagen Holding B.V. Diagnosis of (a risk of ) disease and monitoring of therapy
US20050277124A1 (en) * 2004-06-10 2005-12-15 White Steven M Cardiac conduction system cells and uses thereof
WO2007093412A2 (en) * 2006-02-16 2007-08-23 Fondazione Centro San Raffaele Del Monte Tabor Skeletal muscle periangioblasts and cardiac mesoangioblasts, method for isolation and uses thereof
US9765298B2 (en) * 2006-07-24 2017-09-19 Mayo Foundation For Medical Education And Research Methods and materials for providing cardiac cells
WO2008054819A2 (en) * 2006-11-02 2008-05-08 The General Hospital Corporation Cardiovascular stem cells, methods for stem cell isolation, and uses thereof
WO2008060446A2 (en) * 2006-11-09 2008-05-22 The J. David Gladstone Institutes Methods for inducing cardiomyogenesis
US20110110897A1 (en) * 2008-01-11 2011-05-12 Schwarz Richard P Adult Human Cardiac-Derived Progenitor Cells
EP2100954A1 (en) * 2008-03-10 2009-09-16 Assistance Publique - Hopitaux de Paris Method for generating primate cardiac progenitor cells for clinical use from primate embryonic stem cells, and their applications
WO2009145761A1 (en) * 2008-05-27 2009-12-03 Mayo Foundation For Medical Education And Research Methods and materials for using cells to treat heart tissue

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI226440B (en) * 2000-09-08 2005-01-11 Kuo Kwang Biotech Corp Predicting survival of patients with squamous cell carcinoma

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Rajasingh, et al.,"1-15 STAT3-dependent mouse embryonic stem cell different iation into cardiomyocytes analysis of molecular signaling and therapeutic efficacy of cardiomyocyte precommitted mES transplantation in a mouse model of myocardial infarction" , Circulation research, Vol. 101, 910-918, 2007 *

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EP2433125A1 (en) 2012-03-28
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