TWI549697B - Collyrium - Google Patents

Description

Eye wash

The present invention relates to an eye-washing agent, in particular, an eye-washing agent for washing and removing a cosmetic material adhered to an eyeball or an eyelid, and an eye-washing agent for washing and removing pollen adhered to an eyeball or an eyelid.

Eye wash is used to wash the eyes and remove dust or sweat from the eyes to prevent eye diseases.

For example, Patent Document 1 proposes an ophthalmic agent containing a water-soluble polymer, ethanol, 0.005 to 0.040 w/v% menthol, and chlorobutanol, and having an absolute viscosity at 25 ° C of 1.3 to 15 mPa. s. Patent Document 1 describes a carboxyvinyl polymer as one example of a water-soluble polymer, and further describes that the ophthalmic agent contains an eye wash.

Further, Patent Document 2 describes an ophthalmic composition containing a terpenoid, a water-soluble polymer compound, and a topical intoxicating agent. In Patent Document 2, a carboxyvinyl polymer is described as one example of a water-soluble polymer compound, and an eye-washing agent is described as one of the uses of the ophthalmic composition.

However, the eye-washing agents described in these documents cannot provide a sufficient eye-washing effect in practical use.

[Previous Technical Literature] [Patent Literature] [Patent Document 1]

Japanese Patent Laid-Open No. 2006-176501 (Application No. 1, No. 0014) Paragraph, paragraph 0022, etc.)

[Patent Document 2]

Japanese Patent Laid-Open Publication No. 2003-183157 (Patent No. 1, paragraph 0012, paragraph 0028, etc.)

The present invention has been made in an effort to provide an eye-washing agent which is practically capable of obtaining a sufficient washing effect. Further, an object of the present invention is to provide an eye wash for washing a cosmetic which can effectively wash a cosmetic from the eyeball or its surroundings, prevent adhesion to the eyeball or its surroundings, and sufficiently wash and remove it, and An eye-washing agent for pollen washing, which can effectively wash pollen from the eyeball or its surroundings, and prevent it from being adhered to the eyeball or its surroundings to be sufficiently washed and removed.

The inventors of the present invention have repeatedly studied in order to solve the above problems, and have obtained the following findings.

(i) Eyewash containing carboxyvinyl polymer and monoterpene can effectively wash the cosmetic material adhering to or around the eyeball to prevent adhesion to the eyeball (especially the cornea) and its surroundings, and fully wash it. Remove.

(ii) An eye-washing agent containing a carboxyvinyl polymer and a monoterpene can effectively wash the pollen adhering to or around the eyeball to prevent adhesion to the eyeball (especially the cornea) and its surroundings to be sufficiently washed and removed.

(iii) An eye-washing agent containing a carboxyvinyl polymer and a monoterpene is, in particular, a cosmetic material, pollen dust, etc., since the amount of use (one-time use) in contact with the site to be washed is once 500 μl or more. Clean the dirt and prevent it Adhere to the eyeball (especially the cornea) and its surroundings, and wash and remove it thoroughly.

(iv) Since a carboxyvinyl polymer and a monoterpene are used in combination, when the components are used alone, a synergistic effect can be obtained in the eyewashing action.

The present invention has been completed based on the above findings, and provides the following eye wash.

Item 1 An eye-washing agent containing a carboxyvinyl polymer and a monoterpene, and the amount of use in contact with the portion to be washed is 500 μl or more.

Item 2 The eye wash according to Item 1, wherein the carboxyvinyl polymer is contained in an amount of from 0.001 to 10 w/v% of the eye wash.

Item 3 The eye wash according to Item 1 or 2, wherein the monoterpene content is 0.0001 to 0.2 w/v% of the eye wash.

The eye wash according to any one of the items 1 to 3, wherein the monoterpene is composed of menthol, borneol, geraniol, and bergamot oil. At least one selected in the group.

Item 5 A washing agent for eye wash containing a carboxyvinyl polymer and a monoterpene.

Item 6. The eye wash according to Item 5, wherein the carboxyvinyl polymer is contained in an amount of from 0.001 to 10 w/v% of the eye wash.

Item 7 The eye wash according to Item 5 or 6, wherein the monoterpene content is 0.0001 to 0.2 w/v% of the eye wash.

The eye-washing agent according to any one of items 5 to 7, wherein the monoterpene is at least selected from the group consisting of menthol, borneol, geraniol, and citrus oil. 1 species.

Item 9 The eye wash according to any one of the items 5 to 8, wherein the amount of use in contact with the portion to be cleaned is 500 μl or more.

Item 10 An eye wash for pollen washing, which comprises a carboxyvinyl polymer and a monoterpene.

Item 11. The eye wash according to Item 10, wherein the carboxyvinyl polymer is contained in an amount of from 0.001 to 10 w/v% of the eye wash.

Item 12 The eye wash according to Item 10 or 11, wherein the monoterpene content is 0.0001 to 0.2 w/v% of the eye wash.

The eye-washing agent according to any one of the items 10 to 12, wherein the monoterpene is at least selected from the group consisting of menthol, borneol, geraniol, and citrus oil. 1 species.

Item 14 The eye wash according to any one of the items 10 to 13, wherein the amount of use in contact with the portion to be cleaned is 500 μl or more.

Item 15 Use of a composition containing a carboxyvinyl polymer and a monoterpene for the production of an eye-washing agent, wherein the amount of the eye-washing agent to be contacted with the cleaned portion is 500 μl or more.

Item 16 A composition comprising a carboxyvinyl polymer and a monoterpene for use in eye wash, wherein the amount of use in contact with the portion to be washed is 500 μl or more.

Item 17 An eye washing method comprising the step of contacting a composition containing a carboxyvinyl polymer and a monoterpene with a site to be washed, and the amount of use per one time is 500 μl or more.

Item 18 Use of a composition containing a carboxyvinyl polymer and a monoterpene for the manufacture of an eye wash for cosmetic cleansing.

Item 19 A composition containing a carboxyvinyl polymer and a monoterpene for washing a cosmetic adhering to the eyeball or the eyeball and its surroundings.

Item 20 A method of adhering a cosmetic material which adheres to the eyeball or the eyeball and its surroundings, and comprises a step of contacting the eyeball or the eyeball and its surroundings with a composition containing a carboxyvinyl polymer and a monoterpene.

Item 21 Use of a composition containing a carboxyvinyl polymer and a monoterpene for the manufacture of an eye wash for pollen washing.

Item 22 A composition containing a carboxyvinyl polymer and a monoterpene for washing pollen adhering to the eyeball or the eyeball and its surroundings.

Item 23 A method of washing pollen adhered to the eyeball or the eyeball and its surroundings, comprising the step of contacting the composition containing the carboxyvinyl polymer and the monoterpene with the eyeball or the eyeball and its surroundings.

The eye-washing agent of the present invention can apply pollen, dust, cosmetics (mascara, eyeliner, eyeshadow, lotion, lotion, eye cream) adhered to the eyeball (especially the cornea). Eye cream), etc.) The dirt is effectively removed to prevent re-adhesion, and is sufficiently washed and removed.

Although the dirt is moved from the eyeball to the eye-washing agent, in general, the dirt moved into the eye-washing agent is easily adhered to the eyeball or the eyelid, but the eye-washing agent of the present invention can prevent such re-adhesion, and can effectively remove the stain. Things.

In addition, there are cases where the pollen adheres to the eye, or there is a cosmetic such as mascara, eyeliner, or eye shadow, and the use of the eye-washing agent may cause the stain that has been moved to the eye-washing agent to adhere to the eyeball. However, if the eye wash of the present invention is used for eye wash, it is also possible to suppress a lot of makeup that is transferred to the eye wash. Adhesion of the material on the eyeball.

Further, since the eye-washing agent of the present invention contains a composition of a carboxyvinyl polymer and a monoterpene, it is particularly suitable for the cleaning use of the cosmetic or the washing use of the pollen.

Further, since the eye-washing agent of the present invention contains a composition of a carboxyvinyl polymer and a monoterpene, it is possible to obtain a multiplying eye-washing effect when the components are used alone.

Furthermore, the eye wash of the present invention is safe to the eye.

The invention is described in detail below.

The eye-washing agent of the present invention is an eye-washing agent containing a carboxyvinyl polymer and a monoterpene which is in contact with a portion to be washed and used in an amount of 500 μl or more. The part to be cleaned is the part selected by the eye (eyeball) and other peripheral parts of the eyeball.

(carboxyvinyl polymer)

A carboxyvinyl polymer (hereinafter sometimes referred to as "CVP") is a hydrophilic polymer obtained by polymerizing acrylic acid as a main component, and may be either polyacrylic acid or polyacrylic acid.

When polyacrylic acid is used for mixing the components, the pH is adjusted, and as a result, some or all of the obtained composition may become a polyacrylate. Preferably, when the carboxyvinyl polymer and other components are mixed, a low-viscosity polyacrylic acid is used, and the viscosity of the eye wash composition can be improved by adjusting the pH to some or all of the polyacrylate. Further, polyacrylate is preferably used in the mixing.

As the polyacrylate, an alkali metal salt such as a sodium salt or a potassium salt of polyacrylic acid; an amine such as a monoethanolamine salt, a diethanolamine salt or a triethanolamine salt of polyacrylic acid; an ammonium salt of polyacrylic acid; and the like can be used. Among them, an alkali metal salt is preferred.

Further, the polyacrylic acid or a salt thereof may be either a crosslinked type or a non-crosslinked type. However, in view of the fact that the effects of the present invention are easily exhibited, a crosslinked polymer is preferred.

Commercially available products can be used as the carboxyvinyl polymer. Commercially available products, Carbopol (trade name) (Noveon, Lubrizol), Synthalen (trade name), Hiviswako (trade name) (both Wako Pure Chemical Co., Ltd.), Aqupec (trade name), Sumitomo Refinery Company), Junlon (trade name) (Japan Pure Chemical Company), etc.

The carboxyvinyl polymer may be used singly or in combination of two or more.

The content ratio of the carboxyvinyl polymer is preferably from 0.001 to 10 (w/v)%, more preferably from 0.05 to 5 (w/v), based on the total amount of the eye wash. It is about 0.1%, and more preferably about 0.1 to 1 (w/v)%. In the above content ratio range, a sufficient washing effect and a re-adhesion suppressing effect can be obtained. Further, in the above content ratio range, a good feeling of use can be obtained, and the eye wash agent is hard to adhere around the eyeball, and the irritation of the single eye can be effectively suppressed.

(single)

Monoterpenes include monocyclic monoterpenes such as menthol, anethole, and eugenol; dicyclics such as borneol, isoborneol, cineole, and pinene. Monoterpenes, etc.; geraniol, nerol, Non-cyclic monoterpenes such as myrcenol, langelol, linalyl acetate, lavandulol, and the like. A single 萜 can be any of d, l or dl.

As a single sputum, essential oils containing a single sputum can also be used. Examples of such essential oils include peppermint oil, eucalyptus oil, peppermint oil, citrus oil, spearmint oil, rose oil, and the like.

Among them, preferred are menthol, borneol, geraniol, and citrus oil, more preferably menthol, geraniol, and citrus oil, more preferably menthol, and particularly preferably 1-menthol .

Commercial items can be used as the unit. For example, l-menthol, dl-menthol, d-borneol, dl-borneol, geraniol, which are manufactured by Takasago Co., Ltd., Nagaoka Industrial Co., Ltd., and Nippon Seika Co., Ltd., may be used. Eucalyptus, eucalyptus, citrus oil, peppermint oil, etc.

Further, the essential oil can be taken from the plant by a known method. The well-known essential oil recovery method may be a steam distillation method; a method of adsorbing oil by adding a plant to an animal or vegetable oil after deodorization to adsorb an essential oil, and extracting the essential oil with ethanol; and using hexane or benzene as a plant; The organic solvent or the solvent is extracted by a supercritical fluid, and the solvent is dissolved in ethanol, and the ethanol is evaporated to take a solvent extraction method of the residue; a pressing method or the like. Monoterpenes can also be recovered by various chromatographic methods from essential oils.

The single oxime may be used alone or in combination of two or more.

The content ratio of the monoterpene is preferably from 0.0001 to 0.2 (w/v)%, more preferably from 0.001 to 0.15 (w/v)%, based on the total amount of the eye wash. More preferably, it is about 0.003 to 0.1 (w/v)%.

Further, in the range in which the content ratio of menthol is particularly preferable, the total amount of the thaumol is preferably from about 0.0001 to 0.08 (w/v)%, more preferably from 0.001 to 0.05. It is about w/v)%, and more preferably about 0.003 to 0.03 (w/v)%.

In the range of the above content ratio, a sufficient washing effect can be obtained, and the adhesion suppressing effect can be obtained. Moreover, in the range of the above-mentioned content ratio, a good feeling of use can be obtained, and an unpleasant feeling of use due to other components contained in the eye wash can be suppressed, and a sufficient cooling feeling can be obtained.

(optional)

In the eye wash of the present invention, an active ingredient (a pharmacologically active ingredient or a physiologically active ingredient, etc.) which is generally used in an ophthalmic composition can be formulated in addition to the above components. In the field of the eye-washing agent, the ratio of the formulation of the pharmacologically active ingredient or the physiologically active ingredient is known. In the eye-washing agent of the present invention, the ratio of the pharmacologically active ingredient or the physiologically active ingredient can be appropriately set depending on the type and the like. For example, the compounding ratio of the pharmacologically active ingredient or the physiologically active ingredient may be selected from the range of about 0.0001 to 30% by weight, preferably about 0.001 to 10% by weight, based on the total amount of the eye-washing agent.

Further, the eye-washing agent of the present invention may be appropriately selected according to the use or form, and may contain various carriers or additives, depending on the use or form thereof, within the range which does not impair the effects of the invention.

The optional components may be used alone or in combination of two or more.

(other)

The dosage form of the eye wash of the present invention is not particularly limited, and a liquid preparation, Suspensions, emulsions and other dosage forms. In these dosage forms, the water content is usually about 90% by weight or more, preferably about 95% by weight or more. It is preferably a liquid agent.

Further, the pH of the eye-washing agent of the present invention may be from about 3 to 9, preferably from about 5 to 8.5, more preferably from about 5.5 to 8.

Further, the osmotic pressure ratio of the eye-washing agent of the present invention may be from about 0.6 to 1.6, preferably from about 0.7 to 1.5, more preferably from about 0.8 to 1.4, particularly preferably from about 0.8 to 1.2. The osmotic pressure ratio is the ratio of the osmotic pressure of the sample to the osmotic pressure (286 mOsm) of the 0.9 w/v% sodium chloride aqueous solution according to the fifteenth correction of the Japanese Pharmacopoeia. The osmotic pressure is determined by reference to the osmotic pressure described by the Japanese Pharmacopoeia. Determined by the method (freezing point drop method). As the standard solution for measuring the osmotic pressure ratio, a commercially available osmotic pressure ratio standard solution (0.9 w/v% sodium chloride aqueous solution) or the like can be used.

Moreover, the viscosity of the eye-washing agent of the present invention is about 2 to 1100 mPa. s can be, preferably about 4 to 400 mPa. s, more preferably about 10 to 100 mPa. s. Further, the viscosity can be measured by a (3) cone-plate viscometer using the viscosity measurement method described in the general test method of the Japanese Pharmacopoeia. Specifically, as a cone-plate type viscometer, TV-20 (Toki Industries) can be used. The conditions such as the return rotor and the rotation speed are selected according to the instruction manual of the machine, and the viscosity at 25 ° C is measured.

The material of the container for storing the eye-washing agent of the present invention is not particularly limited, and examples thereof include polyethylene terephthalate and polyethylene glycol dicarboxylate (polyethylene). Naphthalate), polyarylate (PAR), polycarbonate, polyethylene, polypropylene, etc., particularly preferably containing polyethylene glycol terephthalate A container of formate and/or polyethylene glycol naphthalate. Moreover, it is preferable that the eye-washing agent of the present invention is filled in a container having a light-shielding, and the container material may be light-shielded by mixing a coloring agent or the like, or may be covered with a shrink film, an outer casing, or the like, and may be shielded from light. The eye wash of the present invention can be kept stable for a long period of time due to being contained in a light-shielding container.

(Production method)

The eye wash of the present invention can be prepared by a conventional method. For example, each component (carboxyvinyl polymer, monoterpene, and other pharmacologically active components, physiologically active components, additives, and the like which can be optionally formulated) is dispersed in a carrier such as water, and then homogenized, dissolved, or emulsified using a homogenizer or the like. It can be prepared by adjusting the pH with a pH adjuster. Further, in the method of sterilizing the preparation, a method such as sterilization in a sterilization pot, filtration sterilization, or the like can be selected.

(Instructions)

The amount of use of the eye-washing agent of the present invention in one contact with the cleaned portion (meaning the amount of use for one eye wash) is usually 500 μl or more, preferably about 1 ml to 10 ml, more preferably It is about 2 ml to 8 ml, and particularly preferably about 3 to 7 ml. If it is in the above range, the stain adhered to the eyeball or the eyelid can be sufficiently removed, and re-adhesion in the eyeball (corneal, conjunctiva, eye mucosa) and its surroundings can be prevented, and the effects of the present invention can be sufficiently exerted. Further, when the temperature is extremely high, the washing effect may be lowered. However, in the above range, a practically sufficient washing effect can be obtained.

The washing may be carried out by bringing the eye-washing agent once in a single use to the eye and its surroundings (eyelids, etc.) once. Specifically, for example, the eye-washing agent is used once in a cup-shaped container, and the edge of the cup is pressed around the eyes. It is sufficient to carry out contact with the eye and its surroundings (eyelids, etc.) once in contact with the eye-washing agent for one use. In this case, a soft cup is used, and it is desirable to wash the eye wash in the cup with a cup by stirring. Used in this manner, the eye wash of the present application does not easily adhere to the removed dirt once it is removed.

The washing is carried out as necessary, and the number of washings in one day is not particularly limited, but is preferably from about 1 to 6 times a day.

Further, the human being used is not particularly limited. Other than normal healthy people, it can also be used by people with dry eye, eye fatigue, and other eye diseases.

(use)

The object to be cleaned of the eye wash of the present invention is not particularly limited, and can be used for cleaning all the dirt adhering to the eyeball and its surroundings (eyelids, etc.). In particular, it is suitable for use as a cosmetic material for washing, that is, an eye wash for removing makeup stains or an eye wash for pollen washing. When it is used for washing the cosmetic material or for washing the pollen, the amount of use per one time is not particularly limited, but is preferably 500 μl or more. The amount of use per one time is more preferably from about 1 ml to about 10 ml, still more preferably from about 2 ml to about 8 ml, and particularly preferably from about 3 to about 7 ml.

(other aspects of the invention)

The present invention comprises the use of a composition comprising a carboxyvinyl polymer and a monoterpene for use in the manufacture of an eye wash, wherein the amount of the eye wash to be contacted with the cleaned portion is 500 μl or more.

Further, the present invention comprises a composition comprising a carboxyvinyl polymer and a monoterpene which is used for eyewashing in contact with a cleaned portion in an amount of 500 μl or more.

Moreover, the present invention includes an eyewash method comprising The step of contacting the carboxyvinyl polymer and the monoterpene composition with the washed portion is used in an amount of 500 μl or more.

Further, the present invention comprises the use of a composition comprising a carboxyvinyl polymer and a monoterpene for the manufacture of a cosmetic or an eye wash for pollen washing.

Further, the present invention comprises a composition comprising a carboxyvinyl polymer and a monoterpene which is used for washing a cosmetic or pollen adhering to the eyeball or the eyeball and its surroundings.

Further, the present invention includes a method of washing a cosmetic or a pollen adhered to an eyeball or an eyeball and its surroundings, the cleaning method comprising: a composition comprising a carboxyvinyl polymer and a monoterpene, and a human eye or an eyeball; The steps of contact around it.

In the use, composition, and eye-washing method, the type and amount of each component in the composition, the dosage form, the pH, the osmotic pressure ratio, and the viscosity; the container, the production method, the method of use, and the object to be washed are as described above.

[Examples]

The embodiments of the present invention are described in detail below, but the present invention is not limited by the examples.

Test 1 (multiplication effect of carboxyvinyl polymer with monoterpene) (1) Method

(1) A rabbit corneal cell strain (SIRC) was seeded in a Petri dish (24-well, Corning, Japan), and cultured to confluent at 37 ° C, 5% CO ₂ , and humidity of 90%. For the proliferation medium, 10 v/v% of bovine fetal serum was added to Medium 199 in a total amount of Medium 199 (Invitrogen, Japan) as a medium. Moreover, the bottom area of this dish is close to the surface area of one eye.

(2) After the proliferation medium in each well is removed, mascara product 1 (Maquillage Full Vision Mascara) (Note 1), mascara product 2 (Shiseido company; Majolica Majorca Mascara) (Note 2), Or mascara product 3 (NLO company; Maybelin Volum Express Magnum Mascara) (Note 3) Disperse in a 1w/v% Tween 20 aqueous solution for 30 minutes, and then dissolve it in HBSS buffer to a solution with a mascara concentration of 0.1 w/v%. 500 μl of this solution was added to each well, and cultured at 37 ° C, 5% CO ₂ for 5 minutes to adsorb mascara to the cells.

(3) The supernatant liquid was sucked and removed from each well, and 500 μl of each test liquid of Tables 1 to 6 shown below was added to each well, and the mixture was shaken at 400 rpm for 30 seconds.

(4) After the test liquid of each well was sucked and removed, each hole was randomly photographed by a 5-point microscope, and the total area of the residual mascara was calculated by the image analysis software (WinR00F).

(Note 1) Ingredients: hydrogenated polyisobutylene, cyclomethicone, dextrin palmitate, trimethylsiloxysilicate, polymethyl sesquioxane (polymethylsilsesquioxane), disteardimonium hectorite, microcrystalline wax, sucrose acetate stearate, PEG-10 polydimethyl fluorenone ( PEG-10 dimethicone), BG (1,3-butanediol, 1,3-butylene glycol), tris(trimethyldecyloxy)decylpropylaminecarboxylic acid polytriglucorate, water, polyethylene, nylon -12, sucrose tetraisostearate (sucrose Tetraisostearate), arginine, PEG-20 castor oil triisostearate, triethoxycaprylylsilane, cerium oxide, tocopherol, BHT , iron oxide, mica.

(Note 2) Ingredients: hydrogenated polyisobutylene, cyclomethanone, dextrin palmitate, sucrose stearate acetate, distearyl dimethyl saponin, tris(trimethyldecyloxy) Mercaptopropyl methacrylate polytriglucorate, Candelila wax, PEG-10 dimethyl fluorenone, nylon-6, BG, water, triisostearic acid PEG-20 hydrogenated ramie oil , tocopheryl acetate, Australian walnut oil, triethoxyoctyl decane, cerium oxide, sucrose tetraisostearate, paraffin wax, microcrystalline wax, tocopherol, BHT, iron oxide, carbon black.

(Note 3) Ingredients: isodecane, beeswax, carnauba Wax, distearyldimethylamine laponite, water, (allyl stearate/VA) copolymer, rice bran wax, Paraffin, modified alcohol, polyvinyl laurate, (VP/eicene) copolymer, propylene carbonate, talc, (ethylenediamine/dipoly linoleate) copolymer, stearic acid PEG-30 glyceride, propylparaben, methylparaben, hydrogenated jojoba oil, water soluble collagen, [+/-] iron oxide, ultramarine, titanium oxide, Mica, chromium oxide, chromium hydroxide, manganese violet, Berlin blue, cerium oxide.

Moreover, in the test of each test liquid shown in Table 1 shown later, the operation of the above (1) to (4) was repeated three times.

The washing rate was calculated by the following formula from the total area of the remaining mascara measured by each test liquid. In the experiment of each test liquid shown in Table 1 after use, Then, the washing rate was calculated from the total area of the residual mascara.

Washing rate (%) = (1 - total area of residual mascara of the experimental or comparative example / total area of residual mascara of the control) × 100

(2) Results (2-1) Peppermint. Mascara products 1. CVP Commodity 1

The composition of each test liquid is shown in Table 1 below. Also, mascara is made by Shiseido's Maquillage Full Vision Mascara.

Menthol is 1-menthol produced by Nagaoka Corporation. The carboxyvinyl polymer was AQUPEC HV-505E (bridge type) manufactured by Sumitomo Seika Co., Ltd. The PBS buffer was D-PBS (code: 16220015) manufactured by KOHJIN-BIO Co., Ltd. The general D-PBS composition is: 0.2 g/L potassium chloride, 0.2 g/L potassium dihydrogen phosphate, 8 g/L sodium chloride, 1.15 g/L disodium hydrogen phosphate (anhydrous).

The results are shown in Figure 1. As can be seen from Fig. 1, when the menthol and the carboxyvinyl polymer are used in combination, the washing rate of the mascara is particularly high when used alone. It can be seen that when the two are used together, the cosmetic material is fully washed and effectively prevented. The re-adhesion of the cornea is stopped, and the multiplication effect can be seen in the washing action.

(2-2) citrus oil. Mascara products 1. CVP Commodity 1

The composition and washing rate of each test liquid are shown in Table 2 below. Also, mascara is made by Shiseido's Maquillage Full Vision Mascara.

Cilan oil is a citrus oil made from Takasago Spice Industry Co., Ltd. The carboxyvinyl polymer was AQUPEC HV-505E (crosslinked type) manufactured by Sumitomo Seika Co., Ltd. The PBS buffer was D-PBS (code: 16220015) manufactured by KOHJIN-BIO Co., Ltd.

It can be seen from Table 2 that the combination of the citrus oil and the carboxyvinyl polymer can also provide a superior washing effect and a re-adhesion inhibiting effect on the cosmetic material adhered to the corneal cells.

(2-3) Cilan oil. Or geraniol. Mascara products 2. CVP Commodity 1

The composition and washing rate of each test liquid are shown in Table 3 below. Also, mascara is made by Shigoldo's Majolica Majorca Mascara.

Cilan oil is a citrus oil made from Takasago Spice Industry Co., Ltd. Geraniol is made from Geraniol pure manufactured by Takasago Perfumery Industries Co., Ltd. The carboxyvinyl polymer was AQUPEC HV-505E (crosslinked type) manufactured by Sumitomo Seika Co., Ltd. The PBS buffer was D-PBS (code: 16220015) manufactured by KOHJIN-BIO Co., Ltd.

It can be seen from Table 3 that the combination of citrus oil or geraniol and a carboxyvinyl polymer can also provide superior washing effect and re-adhesion inhibiting effect on the cosmetic material adhered to the corneal cells. Moreover, the eye-washing agent of the present invention has an excellent washing effect and a re-adhesion inhibiting effect regardless of the type of the mascara cosmetic.

(2-4) Peppermint oil. Mascara products 1. CVP Commodity 2

The composition and washing rate of each test liquid are shown in Table 4 below. Also, mascara is made by Shiseido's Maquillage Full Vision Mascara.

Menthol is 1-menthol produced by Nagaoka Corporation. The carboxyvinyl polymer was Carbopol 981 (crosslinked type) manufactured by Lubrizol. The PBS buffer was D-PBS (code: 16220015) manufactured by KOHJIN-BIO Co., Ltd.

As is apparent from Table 4, the eye-washing agent of the present invention has an excellent washing effect and a re-adhesion inhibiting effect on the cosmetic material which adheres to the corneal cells irrespective of the kind of the carboxyvinyl polymer.

(2-5) citrus oil. Or geraniol. Mascara products 1. CVP Commodity 2

The composition and washing rate of each test liquid are shown in Table 5 below. Also, mascara is made by Shiseido's Maquillage Full Vision Mascara.

Cilan oil is a citrus oil made from Takasago Spice Industry Co., Ltd. Geraniol is made from Geraniol pure manufactured by Takasago Perfumery Industries Co., Ltd. The carboxyvinyl polymer was Carbopol 981 (crosslinked type) from Lubrizol. The PBS buffer was D-PBS (code: 16220015) manufactured by KOHJIN-BIO Co., Ltd.

As is apparent from Table 5, the eye-washing agent of the present invention has an excellent washing effect and a re-adhesion inhibiting effect on the cosmetic material which adheres to the corneal cells regardless of the type of the carboxyvinyl polymer.

(2-6) Peppermint oil. Mascara products 3. CVP Commodity 1 or 2

The composition and washing rate of each test liquid are shown in Table 6 below. Also, mascara is made using NLO's Maybelline Volum Express Magnum Mascara.

Menthol is 1-menthol which is manufactured by Nagaoka Corporation. The carboxyvinyl polymer ¹⁾ was AQUPEC HV-505E (crosslinked type) manufactured by Sumitomo Seika Co., Ltd. The carboxyvinyl polymer ²⁾ was Carbopol 981 (crosslinked type) manufactured by Lubrizol. The PBS buffer was D-PBS (code: 16220015) manufactured by KOHJIN-BIO Co., Ltd.

As is apparent from Table 6, the eye-washing agent of the present invention has an excellent washing effect and a re-adhesion inhibiting effect on the cosmetic material which adheres to the corneal cells irrespective of the type of the mascara and the type of the carboxyvinyl polymer.

Test 2 (evaluation of usage)

(1) A rabbit corneal cell strain (SIRC) was seeded in a Petri dish (12-well, Corning, Japan), and cultured to a fusion at 37 ° C, 5% CO ₂ , and humidity of 90%. For the proliferation medium, 10 v/v% of bovine fetal serum was added to Medium 199 in a total amount of Medium 199 (Invitrogen, Japan) as a medium.

(2) After the proliferation medium in each well was removed, the mascara 1 (Maquillage Full Vision Mascara) was dispersed in a 1 w/v% Tween 20 aqueous solution for 30 minutes, and then dissolved in HBSS buffer to become a mascara concentration of 0.1. w/v% of the solution, 500 μl of the solution was added to each well, and cultured at 37 ° C, 5% CO ₂ for 5 minutes to adsorb the mascara to the cells.

(3) The supernatant liquid was sucked and removed from each well, and each of the prescriptions shown in Table 1 shown in Table 1 was added with a dose of 100 μl, 500 μl, and 5 ml, respectively, and shaken at 400 rpm for 30 seconds (test liquid). .

In the control, 500 μl of the test liquid of the comparative example 1 of the above Table 1 was added to one well, and the mixture was shaken at 400 rpm for 30 seconds.

(4) After the test liquids of the respective wells were sucked and removed, a 5-point microscope photograph was taken from each well, and the total area of the residual mascara was calculated by the image analysis software (WinR00F).

The above operations (1) to (4) were repeated 3 times.

From each test solution (Example 1 (100 μl, 500 μl, 5 ml)), The average value of the total area of the mascara remaining measured in Comparative Example 1 (500 μl) was used to calculate the washing rate of the test solution by the following formula. In addition, the ratio (washing degree) of the washing rate at the time of washing with 500 μl of the prescription of Example 1 was calculated from the washing rate.

Washing rate (%) = (1 - total area of residual mascara of the experimental example 1 (100 μl, 500 μl, 5 ml) / total area of residual mascara of the comparative example 1 (500 μl)) × 100

Washing degree = (washing rate of experimental example 1 (100 μl, 500 μl, 5 ml) / washing rate of Example 1 (500 μl))

The results are shown in Figure 2. As can be understood from Fig. 2, the formulation of Formulation 1 of the present invention showed a high washing effect in both 500 μl and 5 ml amounts.

Test 3 (Evaluation of the effect of pollen)

(1) Rabbit corneal cell strain (SIRC) was seeded in a Petri dish (24 well, Corning, Japan), and cultured to a fusion at 37 ° C, 5% CO ₂ , and humidity of 90%. For the proliferation medium, 10 v/v% of bovine fetal serum was added to Medium 199 in a total amount of Medium 199 (Invitrogen, Japan) as a medium.

(2) After the proliferation medium in each well was removed and sucked, seeded Ai pollen (SIGMA, Cat. P9395-1G) was dispersed in a HBSS buffer to a solution of 2 mg/ml, and cultured at 37 ° C, 5% CO ₂ for 5 minutes. To make pollen adsorb to cells.

(3) The upper liquid is sucked and removed from each hole, and the first shown in Table 1 is added to each hole. 500 μl of each test liquid shown was shaken at 400 rpm for 30 seconds.

(4) After each test liquid of each well was sucked and removed, a 5-point microscope photograph was taken from each well, and the total area of the residual pollen was calculated by the image analysis software (WinR00F).

The above operations (1) to (4) were repeated 3 times.

The average value of the total area of pollen remaining measured by each test liquid (Example Example 1, Comparative Example Prescription 1, Comparative Prescription 2, Control Prescription) was calculated by the following formula: Example Example 1 and Comparative Example Prescription Examples 1 and 2 Wash rate.

Washing rate (%) = 100-100 × (total area of residual pollen in experimental or comparative examples / total area of residual pollen in control)

The results are shown in Figure 3. When menthol is used alone, the washing effect of the control pollen is better than that of the PBS buffer alone, but when used together with the carboxyvinyl polymer, the pollen washing effect is higher than when the carboxyvinyl polymer is used alone. . From this, it can be seen that for the washing of pollen, the synergistic effect can also be seen by the combined use of mint and carboxyvinyl polymer.

[Possibility of industrial use]

The eye-washing agent of the present invention can be suitably used for removing makeup stains, pollen washing, and the like.

Fig. 1 is a graph showing the effect of multiplying carboxyvinyl polymer and menthol in the washing action of the cosmetic of rabbit corneal cells.

Fig. 2 is a graph showing the effect of the amount of use of the eye wash on the washing action of the cosmetic of the rabbit corneal cells.

Fig. 3 is a graph showing the effect of multiplication of carboxyvinyl polymer and menthol in the washing action of washing pollen from rabbit corneal cells.

Claims (18)

An eye-washing agent comprising a carboxyvinyl polymer and a monoterpene, and the amount of use in contact with the portion to be washed is 500 μl or more. The eye-washing agent according to claim 1, wherein the carboxyvinyl polymer is contained in an amount of from 0.001 to 10 w/v% of the eye wash. The eye wash of claim 1, wherein the monoterpene content is 0.0001 to 0.2 w/v% of the eye wash. An eye-washing agent according to claim 1, wherein the monosaccharide is at least one selected from the group consisting of menthol, borneol, geraniol, and mandarin oil. An eye wash for cosmetic preparation containing a carboxyvinyl polymer and a monoterpene, and the amount of use in contact with the portion to be washed is 500 μl or more. The eye wash according to claim 5, wherein the carboxyvinyl polymer is contained in an amount of from 0.001 to 10 w/v% of the eye wash. The eye wash according to claim 5, wherein the content of the monoterpene is 0.0001 to 0.2 w/v% of the eye wash. The eye-washing agent according to claim 5, wherein the monosaccharide is at least one selected from the group consisting of menthol, borneol, geraniol, and citrus oil. An eye-washing agent for pollen washing, which comprises a carboxyvinyl polymer and a monoterpene, and the amount of use in contact with the portion to be washed is 500 μl or more. The eye wash according to claim 9, wherein the carboxyvinyl polymer is contained in an amount of from 0.001 to 10 w/v% of the eye wash. The eyewash as described in claim 9 of the patent application, wherein the content of the monoterpene It is 0.0001 to 0.2 w/v% of the eye wash. The eye-washing agent according to claim 9, wherein the monosaccharide is at least one selected from the group consisting of menthol, borneol, geraniol, and citrus oil. A use of a composition comprising a carboxyvinyl polymer and a monoterpene for use in the manufacture of an eye wash, wherein the amount of the eye wash to be contacted with the cleaned portion is 500 μl or more. A composition comprising a carboxyvinyl polymer and a monoterpene for use in eye wash, wherein the amount of use in contact with the portion to be cleaned is 500 μl or more. A use of a composition containing a carboxyvinyl polymer and a monoterpene for use in the manufacture of an eye wash for cosmetic cleansing, wherein the amount of use in contact with the portion to be washed is 500 μl or more. A composition containing a carboxyvinyl polymer and a monoterpene which is used for washing and adhering a cosmetic material adhering to an eyeball or an eyeball and a periphery thereof, wherein the amount of use in contact with the portion to be washed is 500 μl or more. A use of a composition containing a carboxyvinyl polymer and a monoterpene for producing an eye wash for pollen washing, wherein the amount of use in contact with the portion to be washed is 500 μl or more. A composition comprising a carboxyvinyl polymer and a monoterpene for washing the eyeball or the pollen adhered to the eyeball and the periphery thereof, wherein the amount of use in contact with the portion to be washed is 500 μl or more.