TW202348221A - Cosmetic composition with skin microbiome-friendly properties - Google Patents

Abstract

The present invention relates to the use of a composition with beneficial skin bacteria-friendly properties. The composition of the present invention does not inhibit the growth of beneficial skin bacteria, has beneficial skin bacteria-friendly properties, can maintain or promote the growth of beneficial bacteria without addition of active materials such as prebiotics or probiotics, and therefore can be provided as a microbiome cosmetic composition.

Description

Cosmetic compositions that are friendly to the skin microbiome

The present invention relates to the use of compositions that are compatible with beneficial skin bacteria. The above composition can be provided as a cosmetic product.

In recent years, consumers have paid increasing attention to "microbiome cosmetics" that effectively protect skin that is damaged or weakened by external stimuli and stress such as dust and ultraviolet rays.

Microbiome cosmetics refer to the application of materials that directly or indirectly affect the growth of "resident flora" that inhabit human skin, inducing changes in the skin's bacterial flora (microbiota), thereby maintaining healthy skin Or cosmetics that improve skin condition. According to prior research, skin-resident bacteria play an important role in maintaining the skin immune system and protecting the skin from some environmental hazards including pathogenic bacteria (Byrd, A. L. et al. (2018) Nature Reviews Microbiology, 16(3), 143). Therefore, through research on microbiome cosmetics, it is possible to maintain the balance between the resident flora present on the skin and create an environment conducive to the growth of beneficial bacteria, thereby strengthening skin immunity, reducing acne, improving the skin barrier, etc. (S . Lebeer et al. (2018) BioRxiv. doi: doi.org/10.1101/463307).

There is a growing market demand for microbiome cosmetics with such expertise, and multinational corporations (MNCs) are running their own research institutes or conducting various studies in collaboration with pharmaceutical companies in order to study microbiome cosmetics. However, current research on microbiome cosmetics focuses on the discovery and application of functional materials with "prebiotic" or "probiotic" effects.

Up to now, most of the microbiome cosmetics researched and marketed contain 1) Prebiotics (Prebiotics) in general cosmetic dosage form ingredients (thickening system, emulsifying system, and ingredients required for water/internal phase stabilization), which induce changes in bacterial flora. or unsustainable dietary elements that help the living environment of beneficial bacteria and confer benefits to the host) and 2) probiotics (Probiotics, microorganisms that confer benefits to the host and maintain an appropriate number of individuals while living in symbiosis) products, or in relatively There are also a wide range of products containing 3) postbiotics (substances produced by the metabolism or fermentation of probiotics).

However, the above ingredients such as prebiotics, probiotics and/or postbiotics are ionized or have unclear chemical properties, and have poor compatibility with cosmetic dosage form ingredients. As a result, when used excessively for high efficacy and high added value, the stability of the dosage form will be compromised. decrease, resulting in possible precipitation and phase separation. To prevent this, most microbiome cosmetics contain only minimal amounts of prebiotic, probiotic and/or postbiotic ingredients.

In addition, for dosage form ingredients that account for the majority of cosmetic compositions, due to the lack of prior research on the microbiome, the effects on skin-resident bacteria and interactions with functional ingredients are not yet clear, so they are used without taking these into consideration. When using ingredients in dosage forms, there is a problem that not only the effect of functional ingredients may be reduced, but also the general (moisturizing, calming, etc.) effects of cosmetics may be reduced.

Against this background, the inventors of the present invention made unremitting efforts to confirm the ingredients and compositions of dosage forms that do not hinder the growth of skin-friendly bacteria. As a result, they confirmed that the specific composition does not hinder the growth of skin-friendly bacteria and is therefore compatible with skin-friendly bacteria. and applied it to a microbiome cosmetic composition, thereby completing the present invention.

[Problem to be solved]

It is an object of the present invention to provide a cosmetic composition for maintaining or enhancing beneficial skin bacteria, comprising a surfactant and an oil.

Another object of the present invention is to provide a cosmetic composition for maintaining the balance of the microbiome in the skin, which contains a surfactant and an oil.

Another object of the present invention is to provide a preparation method of a cosmetic composition for maintaining or enhancing skin beneficial bacteria, including: a) the steps of dissolving and dispersing a surfactant; b) adding oil to the dispersed surfactant into the agent and mix to prepare a mixture; and c) put the above mixture into purified water and emulsify. [Technical means to solve problems]

The present invention will be described in detail below. At the same time, each description and embodiment disclosed in the present invention can also be applied to various different descriptions and embodiments. That is, all combinations of various elements disclosed in the present invention belong to the scope of the present invention. In addition, it is not considered that the scope of the present invention is limited by the specific description described below.

One aspect of the invention provides a cosmetic composition for maintaining or enhancing beneficial skin bacteria, comprising a surfactant and an oil.

The term "skin beneficial bacteria" in the present invention refers to bacteria resident in the skin that enhance skin defense by enhancing the skin regeneration effect, enhance skin regeneration by cell membrane destruction (death) and growth inhibition of harmful bacteria, and inhibit inflammatory factors. Expressed to show the effect of calming the skin and strengthening the skin barrier, showing the effect of increasing the moisture content of the skin and inhibiting water loss, and improving the pH environment by making the skin pH slightly acidic to help maintain skin homeostasis. The above-mentioned skin beneficial bacteria may be, for example, Staphylococcus epidermidis , but are not limited thereto.

The cosmetic composition of the present invention may have properties that are compatible with beneficial skin bacteria.

In the present invention, the above-mentioned affinity properties for skin beneficial bacteria may include maintenance of skin beneficial bacteria and/or enhancement of skin beneficial bacteria.

The term "maintaining beneficial skin bacteria" in the present invention refers to assisting the growth of the aforementioned beneficial skin bacteria to maintain a certain number of individuals. For the purpose of the present invention, the maintenance of the above-mentioned skin beneficial bacteria may be the maintenance of Staphylococcus epidermidis, but is not limited thereto.

The term "enhancing skin beneficial bacteria" in the present invention refers to promoting or improving the growth of the aforementioned skin beneficial bacteria. For the purpose of the present invention, the above-mentioned enhancement of beneficial skin bacteria may be the enhancement of Staphylococcus epidermidis, but is not limited thereto.

As an example, in the present invention, the "bacterial growth index (index)" parameter is used to select a composition with a bacterial growth index of -1.00 or more on the first day after inoculation as having the ability to maintain skin beneficial bacteria and/or enhance skin beneficial bacteria. "Cosmetic composition that is friendly to beneficial skin bacteria".

That is, the cosmetic composition of the present invention may contain a surfactant and oil and have a bacterial growth index of -1.00 or more on the first day after inoculation of the bacteria.

The surfactant of the present invention is not limited to this, but as an example, it can be non-ionic (Non-ionic), phospholipid (Phospholipid), non-phospholipid (Non-Phospholipid), etc. As another example, the above-mentioned non-phospholipid The surfactant may be sodium dilauramidoglutamide lysine, inulin lauryl carbamate, etc., but is not limited thereto. For the purposes of the present invention, the above-mentioned surfactants may be non-ionic, phospholipid-based and/or non-phospholipid-based, but may include without limitation surfactants that do not hinder the growth of beneficial bacteria.

However, in the cosmetic composition of the present invention, it is not non-ionic, phospholipid-based and non-phospholipid-based surfactants but surfaces belonging to anionic (Anionic), cationic (Cationic) and amphiphilic (Amphoteric) The active agent may preferably be included at 1% or less, more preferably at 0.1% or less, based on the total weight of the cosmetic composition of the present invention, and most preferably it may be substantially not included.

Examples of the anionic surfactant include cetyl phosphate, and specifically potassium cetyl phosphate, cetyl phosphate, arginine ), Cetyl phosphate, sodium hydroxide, Cetyl phosphate, tromethamine, Sodium methyl stearyl taurate and sodium stearyl glutamate (Sodium stearyl glutamate), etc., but are not limited thereto.

Examples of the above-mentioned cationic surfactant include distearyldimonium chloride and stearamidopropyl dimethylamine, but are not limited thereto.

Examples of the above-mentioned amphiphilic surfactants include, but are not limited to, Lauryl hydroxysultaine and Cocamidopropyl betaine.

In the present invention, as an example, the nonionic surfactant may be a PEG (Polyethylene glycol) system and/or a non-PEG (non-PEG) system, but is not limited thereto.

As an example, the above-mentioned PEG-based nonionic surfactant may be PEG-100 stearate (PEG-100 stearate), steareth-21 (Steareth-21), PEG-40 stearate (PEG -40 stearate) and PEG-60 hydrogenated castor oil (PEG-60 hydrogenated castor oil), etc., but are not limited thereto.

However, as the above-mentioned PEG-based nonionic surfactant, for example, polysorbate 60 (Polysorbate 60), PEG-60 glyceryl isostearate (PEG-60 glyceryl isostearate), PEG-11 methyl ether polydimethyl PEG-11 methyl ether dimethicone, PEG-10 dimethicone and PEG-150 distearate are preferred relative to The total weight of the cosmetic composition of the present invention may be included at 1% or less, more preferably at 0.1% or less, and most preferably at substantially no amount.

As an example, the non-PEG-based nonionic surfactant may be Glyceryl stearate, Cetearyl alcohol, Cetearyl glucoside, or fatty alcohol. Class, alkyl glucoside, Sorbitan olivate, Cetearyl olivate, Sorbitan stearate, Sucrose polystearate Sucrose polystearate, Cetyl palmitate, Polyglyceryl oleate, Polyglyceryl laurate and Polyglyceryl stearate, etc., but Not limited to this.

As an example, the above-mentioned fatty alcohols may be Lauryl alcohol, Myristyl alcohol, Cetyl alcohol, Stearyl alcohol, Arachidyl alcohol, Behenyl alcohol. (behenyl alcohol) and C14-22 alcohols (C14-22 alcohols), etc., but are not limited thereto.

As an example, the alkyl glucoside may be Lauryl glucoside, Myristyl glucoside, Cetearyl Glucoside, Arachidyl glucoside, C12-18 alkyl glucoside (C12-18 alkyl glucoside), C12-20 alkyl glucoside (C12-20 alkyl glucoside), etc., but are not limited thereto.

As an example, the above-mentioned polyglyceryl oleate can be polyglyceryl-2 oleate (Polyglyceryl-2 oleate), polyglyceryl-3 oleate, polyglyceryl-4 oleate, polyglyceryl-5 oleate, polyglyceryl-5 oleate, Glyceryl-6 oleate, polyglycerol-8 oleate, polyglycerol-10 oleate, etc., but are not limited thereto.

As an example, the above-mentioned polyglyceryl laurate may be polyglyceryl-2 laurate (Polyglyceryl-2 laurate), polyglyceryl-3 laurate, polyglyceryl-4 laurate, polyglyceryl-5 laurate, polyglyceryl laurate, polyglyceryl laurate, etc. Glyceryl-6 laurate, polyglyceryl-10 laurate, etc., but are not limited thereto.

As an example, the polyglyceryl stearate may be polyglyceryl-2 stearate, polyglyceryl-3 stearate, polyglyceryl-4 stearate, or polyglyceryl-5 stearate. Fatty acid ester, polyglycerol-6 stearate, polyglycerol-8 stearate, polyglycerol-10 stearate, etc., but are not limited thereto.

However, as the above-mentioned non-PEG-based nonionic surfactant, for example, coco-glucoside (Coco-glucoside) and the like can be preferably contained in an amount of 1% or less based on the total weight of the cosmetic composition of the present invention, and more preferably Good land may be included at 0.1% or less, and optimal land may not be included substantially.

In the present invention, the phospholipid surfactant may be lecithin, hydrogenated lecithin, etc., but is not limited thereto.

In the present invention, the non-phospholipid surfactant may be sodium dilauramidoglutamide lysine, inulin lauryl carbamate, etc. As mentioned before.

The oil of the present invention may not be Diethoxyethyl succinate, Bis-ethoxydiglycol cyclohexane 1,4-Dicarboxylate, or Bis-ethoxydiglycol cyclohexane 1,4-Dicarboxylate. Heptyl undecylenate, Olea europaea (OLIVE) fruit oil or Persea gratissima (avocado) oil. Specifically, the above-mentioned diethoxyethyl succinate, diethoxydiglycol cyclohexane 1,4-dicarboxylate, heptyl undecenoate, olive oil, avocado oil, etc. are preferably relatively The total weight of the cosmetic composition of the present invention can be included at 1% or less, more preferably at 0.1% or less, and most preferably at not substantially included. In addition to the aforementioned oils, the oils of the present invention may include, without limitation, oils that do not hinder the growth of beneficial bacteria.

As examples thereof, the oil of the present invention may be hydrocarbon oil, ester oil, silicone oil, natural oil, etc. in addition to the aforementioned oils, but is not limited thereto.

In the present invention, as an example, the hydrocarbon oil may be C13-15 alkane, hydrogenated polydecene (Hydrogenated polydecene), hydrogenated polyisobutene (Hydrogenated polyisobutene), isododecane (Isododecane), isodecane Isohexadecane, Squalane, Undecane, Tridecane, C13-14 Alkane, C14-17 Alkane, C14 -19 alkane (C14-19 Alkane), C15-19 alkane (C15-19 alkane), etc., but are not limited thereto.

In the present invention, as an example, the ester oil may be Cetyl ethylhexanoate, Hexyldecyl ethylhexanoate, or Isocetyl myristate. , Isotridecyl isononanoate, Pentaerythrityl tetraisostearate, Pentaerythrityl tetraethylhexanoate, Triethylhexanoin, Caprylic acid/caprylic acid Caprylic/capric triglyceride, Coco-caprylate/caprate, Dicaprylyl carbonate, Phytosterol/octyldodecanol laurel Phytosteryl/octyldodecyl lauroyl glutamate, Trimethylolpropane tricaprylate/tricaprate, Dipentaerythrityl hexa C5-9 acid esters ), Diisostearyl malate, Hexyl laurate, Neopentyl glycol diheptanoate, Ethyl isostearate, Meat Isopropyl myristate, Isostearyl isostearate, Octyldodecyl myristate, etc., but are not limited thereto.

However, as the above-mentioned ester oil, for example, diethoxyethyl succinate, diethoxydiglycol cyclohexane 1,4-dicarboxylate, heptyl undecenoate, etc. are preferred compared to the present invention. The total weight of the cosmetic composition of the invention may be included at 1% or less, more preferably at 0.1% or less, and most preferably not substantially included. As mentioned before about this.

In the present invention, as an example, the silicone oil may be Caprylyl methicone, Cyclohexasiloxane, Cyclopentasiloxane, or Dimethylsiloxane. (Dimethiconol), Dimethicone, Methyl trimethicone, Diphenyl dimethicone, Diphenyldimethicone Diphenylsiloxy phenyl trimethicone and phenyl trimethicone, etc., but are not limited thereto.

In the present invention, as an example, the natural oil may be Helianthus annuus (sunflower) seed oil, Limnanthes alba (meadowfoam) seed oil, Macadamia ternifolia seed oil, Simmondsia chinensis (jojoba) seed oil, Avena sativa (oat) kernel oil, shea butter, etc., but are not limited thereto.

However, as the above-mentioned natural oil, for example, olive oil, avocado oil, etc. can be preferably contained at 1% or less, more preferably at 0.1% or less, based on the total weight of the cosmetic composition of the present invention, and most preferably It may not be included substantially. As mentioned before about this.

The cosmetic composition of the present invention may further comprise fatty acids.

In the present invention, the fatty acid may be myristic acid, stearic acid, behenic acid, etc., but may include without limitation fatty acids that do not hinder the growth of beneficial bacteria.

The cosmetic composition of the present invention may add the aforementioned oil and/or fatty acid as one or more components respectively.

The cosmetic composition of the present invention can be prepared into a form selected from solutions, topical ointments, creams, foams, nutritious lotions, soft lotions, facial masks, toners, emulsions, makeup primers, essences, soaps, liquid cleansing materials, Bath agents, sunscreens, sunscreen oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, cleansers containing surfactants, oils, powder foundations, emulsion foundations, Dosage forms in the group consisting of, but not limited to, wax foundations, patches and sprays.

In the present invention, the above-mentioned cosmetic composition may further contain one or more cosmetically acceptable carriers that are blended into general skin cosmetics. As conventional ingredients, oil, water, surfactants, moisturizers, etc. may be appropriately blended. Lower alcohols, thickeners, chelating agents, pigments, preservatives, flavors, etc., but are not limited to these.

As a specific example, the cosmetic composition of the present invention may further include a thickener. The above-mentioned thickener can be, for example, acrylates/C10-30 Alkyl Acrylate Crosspolymer (Acrylates/C10-30 Alkyl Acrylate Crosspolymer), Carbomer, Polyacrylate Crosspolymer-6 ( Polyacrylate Crosspolymer-6) and Xanthan Gum (Xanthan Gum), etc., but are not limited to these.

As another specific example, the cosmetic composition of the present invention may further contain vitamins. The above-mentioned vitamin may be, for example, Panthenol, but is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the dosage form of the cosmetic composition.

When the dosage form of the present invention is an ointment, paste, cream, or gel, as the carrier component, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, and cellulose can be used Derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc., but are not limited to these. These can be used individually or in mixture of 2 or more types.

When the dosage form of the present invention is powder or spray, as the carrier component, lactose, talc, silicon dioxide, aluminum hydroxide, calcium silicate, polyamide powder, etc. can be used. In particular, when it is a spray, it can be additionally Contains propellants such as, but not limited to, chlorofluorocarbons, propane/butane, or dimethyl ether. These can be used individually or in mixture of 2 or more types.

When the dosage form of the present invention is a solution or emulsion, as the carrier component, a solvent, a solubilizer or an opacifying agent can be used, and for example, water, ethanol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzene, etc. can be used. Methanol, benzyl benzoate, propylene glycol, 1,3-butanediol, cottonseed oil, peanut oil, corn germ oil, castor oil, sesame oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan, But not limited to this. These can be used individually or in mixture of 2 or more types.

When the dosage form of the present invention is a suspension, as a carrier component, liquid diluents such as water, ethanol or propylene glycol, such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan may be utilized. Suspending agents of alcohol esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc., but are not limited to these. These can be used individually or in mixture of 2 or more types.

When the dosage form of the present invention is a soap, as the carrier component, alkali metal salts of fatty acids, fatty acid half-ester salts, fatty acid protein hydrolysates (hydrolysate), isethionate (isethionate), lanolin derivatives, fats can be utilized Alcohols, vegetable oils, glycerin, sugar, etc., but are not limited to these. These can be used individually or in mixture of 2 or more types.

The cosmetic composition of the present invention may further contain purified water.

Relative to the total weight of the composition, the content of each component of the surfactant and oil contained in the cosmetic composition of the present invention may be about 0.1 to 30% by weight, specifically about 1 to 10% by weight, about 2% by weight. to 10 wt%, about 3 to 10 wt%, about 4 to 10 wt%, about 5 to 10 wt%, about 1 to 8 wt%, about 2 to 8 wt%, about 3 to 8 wt%, about 4 to 8% by weight, about 5 to 8% by weight, about 1 to 7% by weight, about 2 to 7% by weight, about 3 to 7% by weight, about 4 to 7% by weight, about 5 to 7% by weight, about 1 to 6 % by weight, about 2 to 6 % by weight, about 3 to 6 % by weight, about 4 to 6 % by weight, about 5 to 6 % by weight, about 1 to 5 % by weight, about 2 to 5 % by weight, about 3 to 5 % by weight %, about 4 to 5% by weight, but not limited to this.

In addition, relative to the total weight of the composition, the content of each component of the fatty acid further included in the cosmetic composition of the present invention may be about 0.1 to 30% by weight, specifically about 1 to 10% by weight, but is not limited to this.

In this disclosure, the term "about" may be given before a specific numerical value. The term "about" used in this application includes not only the exact number stated after the term, but also a range that is almost that number or close to that number. Whether it is close to the specific number mentioned or whether it is almost that number can be decided by taking into account the context in which the number is given. As an example, the term "about" may refer to a range of -10% to +10% of a numerical value. As another example, the term "about" may refer to a range of -5% to +5% of a given numerical value. However, it is not limited to this.

All ingredients described in the present invention preferably do not exceed the maximum usage value stipulated in relevant regulations such as cosmetic safety standards (Korean regulations) and cosmetic safety technical specifications (Chinese regulations).

Another aspect of the present invention provides a cosmetic composition for maintaining microbiome balance in the skin, comprising a surfactant and an oil.

The terminology used here is as stated previously.

The composition of the present invention may further comprise fatty acids.

In addition, the composition of the present invention may further contain any one or more selected from thickeners and vitamins.

In the present invention, "microbiome" is a compound word of microbiota and genome, and refers to the microorganisms that coexist in humans, animals and plants, soil, oceans, lakes, rock walls, atmosphere, etc. The whole genome. Among them, the skin microbiome is a group of microorganisms that form a complex interrelationship between microorganisms and microorganisms and between hosts and microorganisms that exist in the skin. It refers to a part of the human microbiome.

The above-mentioned "maintaining the balance of the microbiome in the skin" refers to promoting the activity and survival of beneficial bacteria in the skin while inhibiting the proliferation and/or activity of harmful bacteria in the skin, thereby appropriately maintaining the balance of the microbiome in the skin. Specifically, the above-mentioned maintenance of microbiome balance can be achieved by maintaining or enhancing beneficial skin bacteria. In the present invention, the above-mentioned skin beneficial bacteria may be Staphylococcus epidermidis.

Yet another aspect of the present invention provides a method for preparing a cosmetic composition for maintaining or enhancing beneficial skin bacteria.

Specifically, the above method may include: a) a step of dissolving and dispersing the surfactant; b) adding oil to the above-mentioned dispersed surfactant and mixing to prepare a mixture; and c) putting the above mixture into a pure water and perform the emulsification step.

The terminology used here is as stated previously.

The above-mentioned step a) may include the step of dissolving and dispersing the surfactant.

The above-mentioned dissolution can be performed at a high temperature. As an example, depending on the activation temperature of each surfactant, it can be performed in a temperature range of 50°C to 80°C, but it is not limited thereto.

As an example, the above-mentioned surfactant may be non-ionic, phospholipid-based, non-phospholipid-based, etc. As another example, the above-mentioned non-phospholipid surfactant may be sodium dilaurylamine, glutamine lysine, and chrysanthemum. Powder lauryl carbamate, etc., but are not limited thereto, as described above.

The above step b) may include a step of adding oil as an oil-soluble component to the surfactant dispersed in the above step a) and mixing to prepare a mixture.

At this time, the addition amount of each component of the above-mentioned surfactant and oil can be about 0.1 to 30% by weight, specifically about 1 to 10% by weight, about 2 to 10% by weight, about 3 to 10% by weight, About 4 to 10 wt%, about 5 to 10 wt%, about 1 to 8 wt%, about 2 to 8 wt%, about 3 to 8 wt%, about 4 to 8 wt%, about 5 to 8 wt%, about 1 to 7% by weight, about 2 to 7% by weight, about 3 to 7% by weight, about 4 to 7% by weight, about 5 to 7% by weight, about 1 to 6% by weight, about 2 to 6% by weight, about 3 To 6 wt%, about 4 to 6 wt%, about 5 to 6 wt%, about 1 to 5 wt%, about 2 to 5 wt%, about 3 to 5 wt%, about 4 to 5 wt%, but not limited to this.

The oil may not be diethoxyethyl succinate, diethoxydiglycol cyclohexane 1,4-dicarboxylate, heptyl undecenoate, olive oil, or avocado oil. In addition to the aforementioned oils, the oils of the present invention may include, without limitation, oils that do not hinder the growth of beneficial bacteria.

As examples thereof, the oil of the present invention may be hydrocarbon oils, ester oils, silicone oils, natural oils, etc. in addition to the aforementioned oils, but is not limited thereto, as described above.

In the above step b), fatty acid may be further added as an oil-soluble component and mixed, but it is not limited thereto.

The fatty acid may be myristic acid, stearic acid, behenic acid, etc., but may include without limitation fatty acids that do not hinder the growth of beneficial bacteria, as described above.

In the step b) above, the oil and/or fatty acid may be added as one or more components respectively.

The above-mentioned step c) may include the step of putting the mixture of the above-mentioned step b) into purified water and emulsifying.

Specifically, the mixture can be slowly poured into purified water so that the total weight % reaches 100 weight %, and then emulsified by a homogenizer for about 1 to 10 minutes, but is not limited thereto.

In the above step b) or c), emulsification can be performed by further adding any one or more selected from thickeners or vitamins, but is not limited thereto.

The above method may further include the step of cooling and then discharging the emulsified mixture after step c) above, but is not limited thereto.

The cosmetic composition prepared by the method of the present invention may have properties that are compatible with beneficial skin bacteria, including maintaining or enhancing the effect of beneficial skin bacteria, and may have an effect of maintaining the balance of the microbiome in the skin. [Invention effect]

The composition of the present invention does not hinder the growth of beneficial bacteria on the skin, is compatible with beneficial bacteria on the skin, and can maintain or promote the growth of beneficial bacteria without adding functional materials such as prebiotics or probiotics, so it can be used as a microbiome. Cosmetic compositions are provided.

Yet another embodiment of the present invention provides the use of a cosmetic composition comprising a surfactant and an oil to prepare a composition that maintains or enhances beneficial skin bacteria.

Another embodiment of the present invention provides the use of a cosmetic composition comprising a surfactant and an oil to prepare a composition for maintaining the balance of the microbiome in the skin.

Hereinafter, the present invention will be explained in more detail by examples. However, these embodiments are used to illustrate the present invention, and the scope of the present invention is not limited by these embodiments, and it will be obvious to those with ordinary skill in the technical field to which the present invention belongs.

Preparation example 1. Preparation of compositions for skin beneficial bacteria affinity assessment

In order to evaluate the affinity of surfactants and oil-soluble ingredients in the dosage form for beneficial skin bacteria, the composition was prepared into an oil-in-water dispersion phase as follows.

Specifically, surfactants are classified into seven series according to functional groups as shown in Figure 2 [nonionic (PEG (polyethylene glycol, non-PEG), non-PEG), ionic (anionic, cationic, and Amphipathic) and others (phospholipid, non-phospholipid)], after measurement, dissolve and disperse in the temperature range of 50°C to 80°C according to the activation temperature of each surfactant. Select up to 4 ingredients from a total of 4 series [hydrocarbons, esters, silicones, natural (natural origin)] that classify oil-soluble ingredients (oils) according to functional groups as shown in Figure 3a and Figure 3b. After measuring 5% by weight of the ingredients (up to 20% by weight in the present invention), mix with the surfactant and stir until uniform. The mixture was slowly poured into purified water until the total weight % reached 100% by weight, then emulsified by a homogenizer for about 5 minutes, cooled and then discharged (Figure 1).

Experimental example 1. Evaluate skin affinity for beneficial bacteria

For each composition prepared in Preparation Example 1, the beneficial bacteria used as the affinity evaluation criteria were Staphylococcus epidermidis ( S. epidermidis ), which is a Gram positive bacterium. Staphylococcus epidermidis is a skin-resident bacterium that mostly exists on healthy skin. It is a representative beneficial bacterium that produces peptide antibiotics or PSM (phenol-soluble modulins), etc., thereby improving skin immunity and helping the skin resist external environmental factors. (Parlet, CP et al. (2019) Trends in microbiology, 27(6), 497-507). The affinity to beneficial bacteria was evaluated as follows using the challenge test method (Russell AD, (2003), Int J Cosmet Sci., 25(3), 147-153).

Specifically, Staphylococcus epidermidis was first cultured in a TSA (Tryptic Soy Agar) medium at 37°C for 24 hours, and then inoculated into 50.0 g of each composition prepared in the above Preparation Example 1, Make it reach 1x10 ⁶ ~ 1x10 ⁷ CFU (colony-forming unit)/ml. After inoculation, vortex or use a sterile rod to evenly mix the bacteria in the composition, and store at room temperature after inoculation. Taking into account the usage cycle of cosmetics and other products and the general usage patterns of users, a maximum of 24 hours is set as the time point that needs to be confirmed. Under this judgment, 24 hours after inoculation, 1 ml of the composition to inoculate the bacterial cells was gradually diluted with 0.85% NaCl solution, 100 μl of each diluted solution was sprayed on the TSA medium, and then cultured at 37°C for 48 hours. Count CFU afterwards.

The affinity evaluation criteria are as follows. Using the "bacterial growth index*" parameter, a composition with a bacterial growth index of -1.00 or more on the first day after inoculation is selected as a "skin-beneficial" composition that has the effect of maintaining skin beneficial bacteria or enhancing skin beneficial bacteria. Bacteria-friendly cosmetics".

^* Bacterial growth index = log ₁₀ [{number of viable bacteria (cfu/g)} / {number of inoculated bacteria (cfu/g)}]

As a result, as shown in Figure 2, it was confirmed that surfactants with ionic properties (anionic, cationic, and amphipathic) and many PEG-containing surfactants drastically inhibit the growth of beneficial bacteria. On the contrary, as non-ionic surfactants PEG-based or non-PEG-based surfactants, phospholipid-based surfactants and non-phospholipid-based surfactants (Sodium dilauramidoglutamide lysine) and inulin lauryl carbamate) do not hinder the growth of beneficial bacteria.

In particular, it was observed from the above results that the phospholipid-based surfactant has a significant effect of maintaining or enhancing beneficial bacteria, and thus it was confirmed that it is suitable for a cosmetic composition compatible with skin beneficial bacteria.

In addition, as shown in Figures 3a and 3b, it was confirmed that the oils among the oil-soluble components were diethoxyethyl succinate, diethoxydiglycol cyclohexane 1,4-dicarboxylate Bis-ethoxydiglycol cyclohexane 1,4-Dicarboxylate, Heptyl undecylenate, Olea europaea (OLIVE) fruit oil) or avocado oil (Persea gratissima (avocado) oil) prevent beneficial effects growth of bacteria. On the contrary, it was confirmed that hydrocarbon oil and silicone oil maintained the bacterial growth index above -1.0 until the 1st and 2nd days and did not hinder the growth of beneficial bacteria. Although there are some differences between ester oil and natural oil, the bacterial growth index is greater than -1.0, and most of them do not hinder the growth of beneficial bacteria.

Preparation example 2. Preparation of cosmetic compositions that are compatible with beneficial skin bacteria

A cosmetic composition containing surfactants and oil-soluble ingredients confirmed not to inhibit the growth of beneficial bacteria based on the results of Experimental Example 1 was prepared using the method of Preparation Example 1 above, and the composition of Table 1 below was mixed to prepare Example 1 and Comparison. Examples 1 to 3.

[Table 1] INCI Example 1 Comparative example 1 Comparative example 2 Comparative example 3 A) Surfactant C14-22 alcohols (C14-22 ALCOHOLS) C12-20 alkyl glucosides (C12-20 ALKYL GLUCOSIDE) 2.50 HYDROGENATED LECITHIN 2.50 PEG-60 GLYCERYL ISOSTEARATE 5.00 2.50 Potassium cetyl alcohol phosphate (POTASSIUM CETYL PHOSPHATE) 5.00 2.50 B)Oil-soluble ingredients HYDROGENATED POLYDECENE 5.00 ISODODECANE _ 5.00 Undecane (UNDECANE) Tridecane (TRIDECANE) 5.00 HYDROGENATED POLYISOBUTENE 5.00 OLEA EUROPAEA (OLIVE) FRUIT OIL 5.00 5.00 5.00 DIETHOXYETHYL SUCCINATE 5.00 5.00 5.00 Bis-ETHOXYDIGLYCOL CYCLOHEXANE 1,4-DICARBOXYLATE 5.00 5.00 5.00 HEPTYL UNDECYLENATE 5.00 5.00 5.00 C) Outside of pure water TROMETHAMINE 0.80 0.80 0.80 0.80 ACRYLATES/C10-30 ALKYL ACRYLATE CROSSPOLYMER 0.10 0.10 0.10 0.10 XANTHAN GUM 0.10 0.10 0.10 0.10 Glycerin (GLYCERIN) 8.50 8.50 8.50 8.50 DIPROPYLENE GLYCOL 4.00 4.00 4.00 4.00 PANTHENOL 2.00 2.00 2.00 2.00 WATER to 100 to 100 to 100 to 100

Experimental example 2. Evaluation Example 1 , comparative example 1 to 3 Skin friendly bacteria affinity

The skin beneficial bacteria affinity evaluation of the cosmetic compositions of Example 1 and Comparative Examples 1 to 3 prepared in Preparation Example 2 was performed using the method of Experimental Example 1, and the results are shown in Tables 2 and 3 below.

[Table 2] CFU/g Example 1 Comparative example 1 Comparative example 2 Comparative example 3 Day 0 10,360,000 10,360,000 10,360,000 10,360,000 Day 1 1,300,000 1,000 54,000 10,000

[table 3] bacterial growth index Example 1 Comparative example 1 Comparative example 2 Comparative example 3 Day 0 0 0 0 0 Day 1 -0.90 -4.02 -2.28 -3.02

As shown in Tables 2 and 3 above, the dosage form of Example 1, developed using hydrocarbon oil and nonionic, phospholipid surfactants that are compatible with beneficial bacteria, did not hinder the growth of Staphylococcus epidermidis, whereas the dosage forms of Comparative Examples 1 to 3 did. Growth of Staphylococcus epidermidis (Fig. 4).

From the results of the above experimental examples, it was confirmed that Example 1 does not hinder the growth of beneficial bacteria on the skin, is compatible with beneficial bacteria on the skin, and can maintain or promote the growth of beneficial bacteria without adding functional materials such as prebiotics or probiotics. , and confirmed that it can be provided as a microbiome cosmetic composition.

Based on the above description, those with ordinary knowledge in the technical field to which the present invention belongs can understand that the present invention can also be implemented in other specific ways without changing the technical idea or necessary features of the present invention. In this regard, it should be understood that the embodiments described above are illustrative and not restrictive in all respects. The scope of the present invention should be construed to include the meaning and range of the patent claims described below and all modifications and variations derived from the concepts of equivalents thereof, and not only include the above detailed description.

[Fig. 1] is a schematic diagram of a composition preparation method for evaluation of skin beneficial bacteria affinity. [Fig. 2] Fig. 2 is a graph showing bacterial growth index based on evaluation of skin-friendly bacteria affinity for each surfactant. [Fig. 3a] and [Fig. 3b] are graphs showing the bacterial growth index (index) based on the skin beneficial bacteria affinity evaluation for each oil-soluble component (oil). [Fig. 4] Fig. 4 is a graph showing the evaluation results of skin beneficial bacteria affinity of the cosmetic compositions of Example 1 and Comparative Examples 1 to 3.

Claims (33)

A cosmetic composition for maintaining or enhancing beneficial skin bacteria, comprising a surfactant and an oil. The composition according to claim 1, wherein the above composition further contains fatty acid. The composition according to claim 1, wherein the skin beneficial bacteria is Staphylococcus epidermidis . The composition according to claim 1, wherein the surfactant is any one selected from the group consisting of non-ionic, phospholipid and non-phospholipid. more than one kind, The above-mentioned non-phospholipid surfactant is any one selected from the group consisting of sodium dilauramidoglutamide lysine and inulin lauryl carbamate. above. The composition according to claim 4, wherein the nonionic surfactant is any one selected from the group consisting of PEG (polyethylene glycol) systems and non-PEG (non-PEG) systems. above. The composition according to claim 5, wherein the PEG-based nonionic surfactant is selected from the group consisting of PEG-100 stearate and Steareth-21. , any one or more of the group consisting of PEG-40 stearate and PEG-60 hydrogenated castor oil. The composition according to claim 5, wherein the non-PEG nonionic surfactant is selected from the group consisting of Glyceryl stearate and Cetearyl alcohol. , Cetearyl glucoside, Lauryl alcohol, Myristyl alcohol, Cetyl alcohol, Stearyl alcohol, Arachidyl alcohol , behenyl alcohol, C14-22 alcohols, Lauryl glucoside, Myristyl glucoside, Cetearyl Glucoside ), Arachidyl glucoside, C12-18 alkyl glucoside, C12-20 alkyl glucoside, Sorbitan olivate), Cetearyl olivate, Sorbitan stearate, Sucrose polystearate, Cetyl palmitate , Polyglyceryl-2 oleate (Polyglyceryl-2 oleate), Polyglyceryl-3 oleate, Polyglyceryl-4 oleate, Polyglyceryl-5 oleate, Polyglyceryl-6 oleate, Polyglyceryl- 8-oleate, polyglyceryl-10 oleate, polyglyceryl-2 laurate, polyglyceryl-3 laurate, polyglyceryl-4 laurate, polyglyceryl-5 laurate , Polyglyceryl-6 laurate, Polyglyceryl-10 laurate, Polyglyceryl-2 stearate (Polyglyceryl-2 stearate), Polyglyceryl-3 stearate, Polyglyceryl-4 stearate, Any one or more of the group consisting of polyglyceryl-5 stearate, polyglyceryl-6 stearate, polyglyceryl-8 stearate and polyglyceryl-10 stearate. The composition according to claim 4, wherein the phospholipid surfactant is lecithin or hydrogenated lecithin. The composition according to claim 1, wherein the oil is any one selected from the group consisting of hydrocarbon oil, ester oil, silicone oil and natural oil. above. The composition according to claim 9, wherein the hydrocarbon oil is selected from the group consisting of C13-15 alkane, hydrogenated polydecene, hydrogenated polyisobutene, and isododecane. (Isododecane), Isohexadecane, Squalane, Undecane, Tridecane, C13-14 Alkane, C14-17 Alkane -17 Alkane), C14-19 Alkane (C14-19 Alkane) and C15-19 Alkane (C15-19 Alkane). The composition according to claim 9, wherein the above-mentioned ester oil is selected from the group consisting of Cetyl ethylhexanoate, Hexyldecyl ethylhexanoate, and Isocetyl Myristin. Isocetyl myristate, Isotridecyl isononanoate, Pentaerythrityl tetraisostearate, Pentaerythrityl tetraethylhexanoate, glyceryl triisooctanoate ( Triethylhexanoin), Caprylic/capric triglyceride, Coco-caprylate/caprate, Dicaprylyl carbonate, Phytosterol/octyl Phytosteryl/octyldodecyl lauroyl glutamate, Trimethylolpropane tricaprylate/tricaprate, Dipentaerythrityl six C5-9 acid esters hexa C5-9 acid esters), Diisostearyl malate, Hexyl laurate, Neopentyl glycol diheptanoate, Ethyl isostearate Any one from the group consisting of Ethyl isostearate, Isopropyl myristate, Isostearyl isostearate and Octyldodecyl myristate above. The composition according to claim 11, wherein the above-mentioned ester oil is not diethoxyethyl succinate or Bis- ethoxydiglycol cyclohexane 1,4-Dicarboxylate) or heptyl undecylenate (Heptyl undecylenate). The composition according to claim 9, wherein the silicone oil is selected from the group consisting of Caprylyl methicone, Cyclohexasiloxane, Cyclopentasiloxane, and Polydiethylene. Dimethiconol, Dimethicone, Methyl trimethicone, Diphenyl dimethicone, Any one or more of the group consisting of diphenylsiloxy phenyl trimethicone and phenyl trimethicone. The composition according to claim 9, wherein the natural oil is selected from the group consisting of sunflower seed oil (Helianthus annuus (sunflower) seed oil), Limnanthes alba (meadowfoam) seed oil, macadamia nut seed oil ( Macadamia ternifolia seed oil), jojoba seed oil (Simmondsia chinensis (jojoba) seed oil), oat kernel oil (Avena sativa (oat) kernel oil) and shea butter (Butyrospermum parkii (shea) butter) Any of the above. The composition according to claim 14, wherein the natural oil is not olive oil (Olea europaea (OLIVE) fruit oil) or avocado oil (Persea gratissima (avocado) oil). The composition according to claim 2, wherein the fatty acid is at least one selected from the group consisting of myristic acid, stearic acid and behenic acid. The composition according to claim 1, wherein the composition further contains any one or more selected from the group consisting of thickening agents and vitamins. The composition according to claim 17, wherein the thickener is selected from the group consisting of acrylates/C10-30 Alkyl Acrylate Crosspolymer, Carbomer ), any one or more of the group consisting of Polyacrylate Crosspolymer-6 and Xanthan Gum. The composition according to claim 17, wherein the vitamin is panthenol. The composition of claim 1, wherein the content of each component of surfactant and oil contained in the composition is 1 to 10% by weight relative to the total weight of the composition. A cosmetic composition for maintaining the balance of the microbiome in the skin, comprising a surfactant and an oil. The composition according to claim 21, wherein the above composition further contains fatty acid. The composition according to claim 21, wherein the above-mentioned maintenance of microbiome balance in the skin is achieved by maintaining or enhancing skin beneficial bacteria. The composition according to claim 23, wherein the skin beneficial bacteria is Staphylococcus epidermidis. A method for preparing a cosmetic composition for maintaining or enhancing beneficial skin bacteria, comprising: a) The step of dissolving and dispersing the surfactant; b) adding oil to the dispersed surfactant and mixing to prepare a mixture; and c) The step of putting the above mixture into purified water and emulsifying it. The method according to claim 25, wherein the surfactant is at least one selected from the group consisting of nonionic, phospholipid-based and non-phospholipid-based, The non-phospholipid surfactant is at least one selected from the group consisting of dilaurylamide glutamine lysine sodium and inulin laureth carbamate. The method according to claim 25, wherein the oil is at least one selected from the group consisting of hydrocarbon oil, ester oil, silicone oil and natural oil. The method according to claim 25, wherein the above-mentioned oil is not diethoxyethyl succinate, diethoxydiglycol cyclohexane 1,4-dicarboxylate, heptyl undecenoate, olive oil or avocado oil. The method according to claim 25, wherein in the above step b), fatty acids are further added and mixed. The method according to claim 25, wherein in step b) or c), emulsification is performed by further adding any one or more selected from thickeners or vitamins. The method of claim 25, wherein the skin beneficial bacteria is Staphylococcus epidermidis . A use of a cosmetic composition containing a surfactant and an oil to prepare a composition that maintains or enhances skin beneficial bacteria. A use of a cosmetic composition containing a surfactant and an oil to prepare a composition that maintains the balance of the microbiome in the skin.

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